Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.79498226
Here we demonstrate that the interaction of CD 2 with its ligand lymphocyte function associated antigen 3 ( CD 58 ) is regulated by T cell receptor CD 3 signaling . 0.79498226^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :1.0351552
Besides facilitating cell to cell adhesion , the molecular interactions between CD 2 and its ligand CD 58 ( lymphocyte function associated antigen 3 [ LFA 3 ] ) , as well as between CD11a / 18 ( LFA 1 ) and CD 54 ( intercellular adhesion molecule 1 ) have recently been recognized to participate in lymphocyte activation , recirculation , and effector function , including cytolytic activity towards tumor cells . 1.0351552^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.98101059
Interaction of CD 2 with its ligand lymphocyte function associated antigen 3 induces adenosine 3 ' , 5 ' cyclic monophosphate production in T lymphocytes . 0.98101059^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.51412441
Purified lymphocyte function associated antigen 3 binds to CD 2 and mediates T lymphocyte adhesion . 0.51412441^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.62529193
Monoclonal antibodies ( mAb ) against the adhesion molecules CD 2 and CD 28 are likely to mimic the interaction with their respective physiological ligands [ lymphocyte function associated antigen 3 ( LFA 3 ) / CD58 , CD 59 and CD 48 for CD 2 , B7 / BB1 for CD 28 ] . 0.62529193^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.87844618
These observations suggest that direct interactions between CD 2 and both CD 58 and CD 59 contribute to T cell activation and adhesion . . 0.87844618^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.53990899
The T cell surface molecule CD 2 forms , with its counter receptor CD 58 ( LFA 3 ) , a powerful adhesion / activation pathway . 0.53990899^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.68772913
This effect is not observed with CHO cells expressing CD 48 , a second CD 2 ligand with a low avidity for CD 2 relative to CD 58 . 0.68772913^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.97311396
In solution , the interaction of human CD 2 with its ligand CD 58 is of low affinity ( 500 mM 1 ) and the interaction of rat CD 2 with its ligand CD 48 is of still lower affinity ( 40 mM 1 ) . 0.97311396^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.63546562
Interaction between CD 2 and its counterreceptor , CD 58 ( LFA 3 ) , on opposing cells optimizes immune recognition , facilitating contacts between helper T lymphocytes and antigen presenting cells as well as between cytolytic effectors and target cells . 0.63546562^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.81702316
CD 2 binds to its primary ligand CD 58 ( LFA 3 ) on antigen presenting cells ( APC ) and stabilizes the T cell APC interaction ; this stable interaction then optimizes Ag specific T cell activation . 0.81702316^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.95007863
The results suggest that preincubation of mononuclear cells with anti CD 2 McAb has a down regulating effects on B cell responses and the interaction of CD 2 with its ligand LFA 3 expressed by RBC is closely related to immune responses . . 0.95007863^^^ Potential effect of the interaction of CD 2 with its ligand LFA 3 on B cell responses in vitro was studied to evaluate the action of CD 2 LFA 3 in immune responses . 0.90281627^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.62011277
These studies suggest that interaction of CD 2 with its natural ligand , LFA 3 , may play a role in regulation of HIV expression . . 0.62011277^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.63769831
Monoclonal antibody inhibition of spontaneous rosetting indicated that it is not dependent on the T cell receptor complex but is largely mediated by interaction of T cell CD 2 ( T11 / E rosette receptor ) with its ligand LFA 3 on L 428 cells . 0.63769831^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.73050785
Interaction of CD 2 with its ligand , LFA 3 , in human T cell proliferation . 0.73050785^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.77933145
We now report that CD 2 binds to a cell surface antigen known as lymphocyte function associated antigen 3 ( LFA 3 ) with high affinity , and can mediate adhesion of lymphoid cells via interaction with LFA 3 . . 0.77933145^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.50267742
Recently the human T cell erythrocyte receptor CD 2 has been shown to bind human erythrocytes through LFA 3 , a heavily glycosylated surface protein of broad tissue distribution . 0.50267742^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.8191376
In this report , we show that specific interactions of LFA 3 with CD 2 can result in T cell unresponsiveness to antigenic or mitogenic stimuli in vitro . 0.8191376^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.57666178
Interaction of the T cell CD 2 receptor with its natural ligand LFA 3 expressed on stromal cells might represent a regulatory mechanism for rapid release of IL 3 , facilitating proliferation of multipotent hematopoietic cells . . 0.57666178^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.60313565
Visualization of CD 2 interaction with LFA 3 and determination of the two dimensional dissociation constant for adhesion receptors in a contact area . 0.60313565^^^ We have studied the interaction of CD 2 with LFA 3 in the contact area between Jurkat T lymphoblasts and planar phospholipid bilayers containing purified , fluorescently labeled LFA 3 . 0.55286251^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Enhancement of T cell adhesiveness through CD 44 engagement was not mediated by a direct effect on lymphocyte function associated Ag 3 , the known ligand of CD 2 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
To define epitopes on the CD 2 ( T 11 , the T cell erythrocyte receptor ) molecule that are necessary for interaction with lymphocyte function associated Ag 3 ( LFA 3 ) , we have expressed the human wild type CD 2 cDNA and mutant CD 2 cDNA in a murine Ag specific T cell hybridoma that responds to human HLA DR Ag . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Although the exact function of CD 2 is unknown , lymphocyte function associated Ag 3 ( LFA 3 ) , a 55 to 70 kDa receptor expressed on a broad spectrum of hemopoietic and nonhemopoietic cells , has recently been shown to be its natural ligand . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Moreover , cells reactive with anti CD 2 antibodies against T 11 ( 2 ) and T 11 ( 3 ) epitopes of CD 2 as well as thymic stromal cells expressing the CD 2 ligand , lymphocyte function associated Ag 3 , were also present at 8 . 5 wk . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Through physiologic interactions with its ligands CD 58 ( lymphocyte function associated Ag 3 , LFA 3 ) and CD 59 , the T cell glycoprotein CD 2 plays a role in T cell signaling and promotes lymphocyte adhesion . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
We previously reported that human umbilical vein EC provide costimulatory signals to PHA stimulated PBL via CD 2 : lymphocyte function associated Ag 3 and an unidentified ligand pair , resulting in a three to eight fold enhancement of IL 2 production . ^^^ This effect is largely independent of the CD 2 : lymphocyte function associated Ag 3 pathway . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The 60 kDa lymphocyte function associated Ag 3 ( LFA 3 / CD58 ) , a highly glycosylated adhesion molecule that serves as ligand for the T cell restricted glycoprotein CD 2 , is encoded by a gene at the human chromosome locus 1p13 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Effect of lengthening lymphocyte function associated antigen 3 on adhesion to CD 2 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
On AC expressing high levels of CD 54 ( intercellular adhesion molecule 1 , ICAM 1 ) and CD 58 ( lymphocyte function associated antigen 3 , LFA 3 ) , mAb to CD 2 were relatively ineffective in inhibiting the response to the toxins and antibodies to the lymphocyte function associated antigen 1 ( LFA 1 ) did not inhibit at all . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
In particular , we investigated the adhesion between a Jurkat cell expressing CD 2 and a glass supported planar bilayer containing either the glycosyl phosphatidylinositol ( GPI ) or the transmembrane ( TM ) isoform of the counter receptor lymphocyte function associated antigen 3 ( LFA 3 ) at a concentration of 1 , 000 molecules / microns 2 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
EC achieve these effects in part by utilizing lymphocyte function associated antigen 3 ( LFA 3 ) to interact with T cell CD 2 as shown by observations that EC augmentation of IL 2 is partially ( 50 70 % ) blocked by eight of eight mAb tested which recognize LFA 3 ; that purified phosphatidylinositol linked LFA 3 ( PI LFA 3 ) can also provide costimulation to CD4+ T cells ; and that there is a delay of the EC effect on CD4+ T cells which express low levels of CD 2 compared to those which express high levels of CD 2 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
In this report we compare the effect of stimulation of peripheral mononuclear cells ( PBMC ) by using two monoclonal antibodies ( MoAb ) directed against the CD 2 receptor on T cells or by using autologous erythrocytes ( E ) which express on their surface lymphocyte function associated antigen 3 ( LFA 3 ) , a natural ligand for CD 2 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Role of interaction of CD 2 molecules with lymphocyte function associated antigen 3 in T cell recognition of nominal antigen . ^^^ The role of the interaction of CD 2 molecules with lymphocyte function associated antigen 3 ( LFA 3 ) in facilitating nominal antigen recognition by T lymphocytes was studied by utilizing an HLA DR 4 restricted CD4+ cytotoxic human T cell clone specific for human immunodeficiency virus envelope glycoprotein gp 120 as a responder and murine fibroblasts transfected with human class 2 major histocompatibility complex ( MHC ) and / or human LFA 3 molecules as antigen presenting cells ( APC ) . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
This latter family includes another adhesive moiety , namely , the lymphocyte function associated antigen 3 ( LFA 3 ) , which is the ligand for the T lymphocyte associated CD 2 molecule , and has a broad tissue and organ distribution . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Since the initiation of a specific immune response also involves antigen receptor independent interactions between accessory molecules , such as lymphocyte function associated antigen 1 ( LFA 1 ) with intercellular adhesion molecule 1 ( ICAM 1 ) and lymphocyte function associated antigen 3 ( LFA 3 ) with CD 2 , it was of interest to determine whether TEC can express the adhesion molecules ( ICAM 1 and LFA 3 ) which augment the efficiency of antigen presentation . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
CD 2 ( E receptor , LFA 3 receptor ) and E 2 molecules ( Bernard , 1988 ) on human T lymphocytes , CD 58 ( LFA 3 , lymphocyte function associated antigen 3 ) on human erythrocytes and S 14 , S 42 , S 110 220 molecules ( Bernard , 1987 ) of sheep erythrocytes are involved in rosette formation of human T lymphocytes with human or sheep erythrocytes . ^^^ CD 2 ( E receptor , LFA 3 receptor ) and E 2 molecules ( Bernard , 1988 ) on human T lymphocytes , CD 58 ( LFA 3 , lymphocyte function associated antigen 3 ) on human erythrocytes and S 14 , S 42 , S 110 220 molecules ( Bernard , 1987 ) of sheep erythrocytes are involved in rosette formation of human T lymphocytes with human or sheep erythrocytes . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
We have used chemically cross linked dimers , trimers , and tetramers of lymphocyte function associated antigen 3 ( LFA 3 ) to study the role of multivalency in the interaction of the protein with its receptor , CD 2 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Expression of the T cell surface molecule CD 2 and an epitope loss CD 2 mutant to define the role of lymphocyte function associated antigen 3 ( LFA 3 ) in T cell activation . ^^^ To define the role of the CD 2 lymphocyte function associated antigen 3 ( LFA 3 ) interaction in T cell activation , we have expressed a cDNA encoding the human CD 2 molecule in a murine antigen specific T cell hybridoma . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Monoclonal antibodies ( MAbs ) directed against CD 3 , CD 8 , CD 2 , and lymphocyte function associated antigen 3 , but not against CD 5 , inhibited cytolytic activity . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Antibodies against certain epitopes of the T cell CD 2 ( 35 . 1 ) and synovial cell lymphocyte function associated antigen 3 ( LFA 3 ) ( TS2 / 9 ) molecules inhibited synovial cell thymocyte binding . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Lymphocyte function associated antigen 3 ( LFA 3 ) is a widely distributed cell surface glycoprotein that has been assigned a role in cell cell adhesion on the basis of its capacity to bind to the T lymphocyte CD 2 antigen . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Purified lymphocyte function associated antigen 3 ( LFA 3 ) activates human thymocytes via the CD 2 pathway . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
A natural ligand for CD 2 has been found to be lymphocyte function associated antigen 3 ( LFA 3 ) , a widely distributed cell surface glycoprotein . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
In this review Malegapuru Makgoba , Martin Sanders and Stephen Shaw focus primarily on the two molecular pathways of lymphocyte adhesion that have been shown to play a critical role in facilitation of antigen specific recognition , namely CD 2 and its ligand , lymphocyte function associated antigen 3 ( LFA 3 ) , and LFA 1 and its ligand , intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Purified lymphocyte function associated antigen 3 and T 11 target structure are active in CD 2 mediated T cell stimulation . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Definition of the receptor and ligand molecules as CD 2 and lymphocyte function associated antigen 3 ( LFA 3 ) . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Monoclonal antibodies to CD 2 and lymphocyte function associated antigen 3 inhibit human thymic epithelial cell dependent mature thymocyte activation . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Lymphocyte function associated antigen 3 ( LFA 3 ) is a widely distributed cell surface glycoprotein that binds to the T lymphocyte CD 2 surface glycoprotein . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
We have isolated the cDNA for human lymphocyte function associated antigen 3 ( LFA 3 ) , the ligand of the T lymphocyte CD 2 molecule . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Direct evidence for a receptor ligand interaction between the T cell surface antigen CD 2 and lymphocyte function associated antigen 3 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Amino acid residues required for binding of lymphocyte function associated antigen 3 ( CD 58 ) to its counter receptor CD 2 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
To search for possible ligands of CD 2 distinct from CD 58 ( lymphocyte function associated antigen 3 ) , we have produced a soluble pentameric CD 2 immunoglobulin ( Ig ) fusion protein ( spCD 2 ) linking the 182 amino acid human CD 2 extracellular segment with CH 2 CH3 CH 4 domains of human IgM heavy chain , thus enhancing the micromolar affinity of the CD 2 monomer through multimeric interaction . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The adhesion interaction between the immunoglobulin superfamily molecules CD 2 and CD 58 ( lymphocyte function associated antigen 3 ) plays an important role in T cell and natural killer cell interaction with various antigen presenting and target cells . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
This potentiation has been previously shown to be dependent on interactions between the CD 2 molecule on T cells and the lymphocyte function associated antigen 3 ( LFA 3 ) expressed by autologous erythrocytes . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Using both cellular and molecular biological approaches , we have mapped the portions of CD 2 involved in CD 2 dependent signaling and in the regulation of avidity for its ligand CD 58 ( lymphocyte function associated antigen 3 , LFA 3 ) to structurally distinct portions of the cytoplasmic domain . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The rosetting of T cells by sheep erythrocytes is mediated through the interaction of the CD 2 molecule on T cells with T11TS , a molecule on sheep erythrocytes homologous to lymphocyte function associated antigen 3 ( LFA 3 , CD 58 ) . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
We have previously reported that costimulatory pathways including B 7 CD28 and lymphocyte function associated antigen 3 ( LFA 3 ) CD 2 shape distinct activation profiles in human CD4+ T cells . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Crystal structure of the CD 2 binding domain of CD 58 ( lymphocyte function associated antigen 3 ) at 1 . 8 A resolution . ^^^ The binding of the cell surface molecule CD 58 ( formerly lymphocyte function associated antigen 3 ) to its ligand , CD 2 , significantly increases the sensitivity of antigen recognition by T cells . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
TNF alpha has been validated as a good treatment target but the potential modalities also include the inhibition of the interaction between LFA 3 ( lymphocyte function associated antigen 3 ) and CD 2 , the blockade of the IL 1 receptors , the antibodies against the alpha 4 integrins , the antibodies against B cell CD 20 and the inhibition of the activation of T cells . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
CD 2 binds specifically to the surface glycoprotein LFA 3 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Lymphocytes and the H 2 tissue expressed adhesion molecule subunits for the following potential cell cell or cell matrix interactions : alpha 3 beta 1 ( VLA 3 ) to fibronectin , laminin , and collagen ; alpha 4 beta 1 ( VLA 4 ) and alpha 5 beta 1 ( VLA 5 ) to fibronectin ; alpha 6 beta 1 ( VLA 6 ) to laminin ; alpha 4 beta 1 to VCAM 1 ; alpha L beta 2 ( Leu CAMa / LFA 1 ) to CD 54 ( ICAM 1 ) ; CD 44 to fibronectin , collagen , laminin , hyaluronate ; CD 2 to CD 58 ( LFA 3 ) ; and CD 56 ( N CAM ) to CD 56 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Both 5117GB and 5117GBT were positive for laminin ( 25 33 % ) , CD 2 ( LFA 3 receptor , 95 98 % ) , CD 54 ( ICAM 1 , 99 100 % ) and CD 58 ( LFA 3 , 100 % ) . 5117GB was positive for HLA ABC while 5117GBT lost detectable HLA ABC . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The expression of HLA class 1 , HLA DR , HLA DP , HLA DQ , intercellular adhesion molecule 1 ( ICAM 1 / CD54 ) , lymphocyte function associated ( LFA ) molecules LFA 1 ( CD11a / CD18 ) , LFA 2 ( CD 2 ) , and LFA 3 ( CD 58 ) was investigated by FACS analysis before and after induction for various times with human rIFN gamma , TNF alpha , or both . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Binding of CD 2 to its ligand CD 58 was the critical event mediating stimulation because : ( a ) monoclonal antibodies ( mAbs ) to CD 2 and to CD 58 , but not to a variety of other surface structures , blocked induced and spontaneous proliferation and IL 2 production ; ( b ) only anti CD 2 mAbs were stimulatory if crosslinked ; ( c ) a nonstimulatory cell was rendered stimulatory by CD 58 transfection ; and ( d ) the cells responded specifically to CD 58 on sheep red blood cells . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The antigens investigated were CD 2 and its ligand , LFA 3 ( CD 58 ) . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Whereas PBL showed bimodal expression profiles of CD45R0 , CD 58 , and CD 2 , the vast majority of LPL was bright for these Ag . ^^^ CD45R0 bright T cells from the peripheral blood ( PB T ) were predominantly bright for CD 2 , CD 58 , CD 29 , and CD11a / CD18 whereas CD45R0 dim PB T had bimodal expression profiles and CD45R0 negative PB T were dim or even negative for these Ag . ^^^ CD45R0 bright LP T were also bright for CD 2 and CD 58 but had significantly reduced surface densities of CD11a / CD18 and CD 29 compared with CD45R0 bright PB T . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
In the study reported here , the distribution of the adhesion receptors LFA 1 ( CD11a / CD18 ) , ICAM 1 ( CD 54 ) , CD 2 and LFA 3 ( CD 58 ) in recurrent oral ulcers ( ROU ) were studied . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Whereas several epitopes and their respective function are known for CD 2 , a similarly detailed characterization of CD 58 is still lacking . ^^^ Two of these epitopes represent functionally relevant sites , involved in binding of CD 58 to CD 2 and T cell activation via CD 2 . ^^^ The new mAb allow for the first time the detection of CD 58 in enzyme linked immunosorbent assays and immunofluorescence while bound to its receptor CD 2 in human serum or on freshly isolated blood cells . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The expression of the immunoglobulin related molecules CD 2 , CD 54 ( ICAM 1 ) and CD 58 ( LFA 3 ) was higher on RA SFL when compared to RA PBL or CO PBL , and similar results were obtained with the beta 2 integrin subunits CD11a and CD 18 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The interaction between the E rosette receptor ( CD 2 ) expressed on the cell surface of T lymphocytes and its natural ligand CD 58 ( LFA 3 ) , a broadly distributed cell surface molecule , represents a potential target for immune modulation . ^^^ To explore this possibility , we have expressed a soluble recombinant form of human CD 58 which exerts potent biological effects in that it acts as a competitive inhibitor for cell surface CD 58 in its binding to CD 2 positive lymphocytes . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The T lymphocyte glycoprotein receptor , CD 2 , mediates cell cell adhesion by binding to the surface molecule CD 58 ( LFA 3 ) on many cell types including antigen presenting cells . ^^^ Analysis of deglycosylated soluble recombinant CD 2 as well as a mutant transmembrane CD 2 molecule containing a single Asn 65 Gln65 substitution demonstrates that neither deglycosylated CD 2 nor the mutant CD 2 transmembrane receptor binds CD 58 or monoclonal antibodies directed at native CD 2 adhesion domain epitopes . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The expression of CD 58 after culture is in accordance with the interaction of LC with CD 2 bearing T lymphocytes during antigen presentation in peripheral lymph nodes . . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Antibodies specific for CD 2 or LFA 3 ( CD 58 ) did not affect the VLA 4 mediated cell adhesion . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Activation with anti CD 2 plus anti CD 28 mAb resulted in major changes in the cell surface phenotype and functional properties : a loss of CD45RA+ occurred and an increased expression of CD45RO , CD 29 , and CD 58 ( LFA 3 ) , as well as a gain in responsiveness to anti CD 3 and anti CD 2 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Pretreatment of T lymphocytes with monoclonal antibodies directed at either CD11a / CD18 ( LFA 1 ) or CD 2 molecules or that of EC with monoclonal antibodies directed at either CD 54 ( ICAM 1 ) or CD 58 ( LFA 3 ) molecules significantly inhibited T lymphocyte induced enhancement in endothelial permeability , thus indicating that activated T lymphocytes utilize both type 1 ( CD11a / CD18CD54 ) and type 2 ( CD2CD58 ) intercellular adhesion pathways to augment endothelial permeability and signals received via CD 3 or CD 28 molecules on T lymphocytes further enhance this process . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The molecules studied included CD 3 , CD45R , UCHL 1 ( CD45RO ) , lymphocyte function associated antigen 1 ( LFA 1 ) ( CD11a , CD 18 ) , intercellular adhesion molecule 1 ( ICAM 1 ) ( CD 54 ) , 4B4 ( CD 29 ) , CD 44 , CD 2 , and LFA 3 ( CD 58 ) . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
After in vitro incubation with IL 2 , human NK cells demonstrated four to sixfold increases in surface levels of CD11a / CD18 , CD 2 , CD 54 , CD 58 , and the NK cell associated Ag NKH 1 ( CD 56 ) . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The complementary adhesion molecules LFA 1 ( CD11a , 18 ) / ICAM 1 ( CD 54 ) and LFA 2 ( CD 2 ) / LFA 3 ( CD 58 ) have been shown to be important in T cell interaction with lymphoid target cells . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
T cell responsiveness to both IL 1 and IL 6 was facilitated in the presence of CD 58 like signals as delivered by human rCD 58 , SRBC or a mAb ( anti T111A ) , which binds to an interaction site for CD 58 on the human CD 2 molecule . ^^^ These findings indicate that CD 2 and its ligand CD 58 play an important role in T cell / monocyte interactions during primary immune responses by means of upregulating T cell susceptibility to monocyte derived cytokines . . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Inhibition of the growth of normal granulocyte macrophage colonies by CD2+ LAK cells is blocked by antibodies to the CD 2 lymphocyte function associated antigen 3 ( LFA 3 ) ( CD 58 ) cell adhesion system . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Dual function of recombinant human CD 58 : inhibition of T cell adhesion and activation via the CD 2 pathway . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The possible role ( s ) of the adhesion molecules LFA 1 alpha ( CD11a ) , LFA 1 beta ( CD 18 ) , ICAM 1 ( CD 54 ) , CD 2 ( T 11 , LFA 2 ) , and LFA 3 ( CD 58 ) in the in vitro activation of nickel specific peripheral blood ( PB ) T lymphocytes was studied . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Resting CD45RO+ , mature / memory , T cells are phenotypically distinct from intermediate CD45RO+ / CD45RA+ and CD45RA+ , immature / virgin , T cells , and are characterized by high levels of expression of a number of adhesion molecules , such as CD 2 , CD 18 , CD 58 and CD 29 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Human thymic epithelial cells grown in vitro bind to mature and immature thymocytes via CD 2 and CD11a / CD18 ( LFA 1 ) molecules on thymocytes and by CD 58 ( LFA 3 ) and CD 54 ( ICAM 1 ) molecules on thymic epithelial cells . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
There are two molecular pathways of leucocyte adhesion the binding of CD11a c / CD18 ( Leu CAM family ) on leucocytes to CD 54 ( ICAM 1 ) on mononuclear leucocytes , fibroblasts , and epithelial and vascular endothelial cells , or of CD 2 on T cells to CD 58 , a cell surface molecule found on a broad range of tissue cells . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The CD 58 ( LFA 3 ) binding site is a localized and highly charged surface area on the AGFCC ' C ' ' face of the human CD 2 adhesion domain . ^^^ Using site directed mutagenesis in conjunction with NMR structural data on the adhesion domain of human CD 2 , we have defined the binding region for CD 58 . ^^^ Here we report that the CD 58 binding site is a well circumscribed , charged surface area covering approximately 770 A 2 on the AGFCC ' C ' ' face of the CD 2 beta barrel . ^^^ In contrast , neither residues on the more peripheral G and C ' ' strands of the same CD 2 surface nor residues on B , E , and D strands of the opposite face are involved in CD 58 binding . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
IFN gamma alone or in combination with TNF alpha strongly enhanced expression of HLA class 1 , HLA DR , DP , DQ , LFA 1 alpha and ICAM 1 , but did not affect expression of LFA 2 ( CD 2 ) and LFA 3 ( CD 58 ) . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
In addition , experiments in which either purified ligand ( CD 58 ) or transfected CD 2 and CD 58 molecules were used have confirmed this notion . ^^^ However , controversy exists as to whether CD 2 alters its affinity for CD 58 in the course of T cell stimulation , and whether this putative affinity change affects CD 2 mediated activation signals . ^^^ We now describe a CD 2 mAb ( HIK 27 ) that recognizes an epitope constitutively expressed on resting T cells and induces increased adhesiveness of CD 2 toward CD 58 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Here we show that ligation of B cell CD 58 by CD 2 , its natural ligand on T cells , or by mAb , provides a novel IL 4 dependent signal for the latter two steps . ^^^ Thus , the CD 2 : CD 58 ligand / counterligand system provides an alternative pathway by which cell contact signaling may regulate IgE . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Another adhesion pathway is mediated by CD 2 and CD 58 . ^^^ CD 2 , a glycoprotein selectively expressed by T cells , is a receptor for CD 58 , a cell surface adhesive ligand with broad tissue distribution . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
MAbs specific to other adherence molecules such as ICAM 1 , CD 2 , CD 58 , CD 4 , CD 8 , CD11b and LAM 1 did not affect leukocyte adherence in this model . . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Human cell adhesion molecule CD 2 binds CD 58 ( LFA 3 ) with a very low affinity and an extremely fast dissociation rate but does not bind CD 48 or CD 59 . ^^^ Reported ligands for human CD 2 include the structurally related IgSF CAMs CD 58 ( LFA 3 ) and CD 48 as well as , more controversially , the unrelated cell surface glycoprotein CD 59 . ^^^ In contrast , a study using conventional equilibrium binding methods reported a much higher affinity ( Kd 0 . 4 microM ) for human CD 2 binding CD 58 which suggested that the weak binding of rat CD 2 to CD 48 may not represent a typical CAM interaction . ^^^ In the present study we have used surface plasmon resonance to obtain definitive affinity and kinetic data on the interactions of a soluble , recombinant form of human CD 2 with soluble forms of CD 58 , CD 48 , and CD 59 . ^^^ Binding of CD 2 to CD 58 was readily detected but we were unable to detect any direct interaction between CD 2 and either CD 59 or CD 48 under conditions in which very low affinity interactions ( Kd approximately 0 . 5 mM ) would have been detected . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The ICAM 3 induced cell aggregation of JM and HAFSA cells was not affected by the addition of blocking mAb specific for a number of cell adhesion molecules such as CD 1 1a / CD18 , ICAM 1 ( CD 54 ) , CD 2 , LFA 3 ( CD 58 ) , very late antigen alpha 4 ( CD49d ) , and very late antigen beta 1 ( CD 29 ) . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
CD 58 is the principal ligand for human CD 2 ; CD 59 and CD 48 are two additional , low affinity ligands that have been defined for CD 2 . ^^^ We have shown that CD 59 enhanced CD 58 dependent T cell proliferation and IL 2 secretion in the presence of suboptimal concentration of PHA or a submitogenic combination of stimulatory anti CD 2 mAbs , presence of suboptimal concentration of PHA or a submitogenic combination of stimulatory anti CD 2 mAbs , T 11 2 + T 11 3 . ^^^ As expected , CD 59 costimulation of CD 58 dependent T cell proliferation was inhibited by Abs directed against CD 59 , CD 58 , and CD 2 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
With the identification of murine CD 48 as a homolog of the human CD 2 ligand LFA 3 ( CD 58 ) and as a ligand itself for murine CD 2 , the anti murine CD 48 mAb HM 48 1 was administered intravenously to investigate the role of CD 48 in cell mediated immunity in vivo . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Interaction between human CD 2 and CD 58 involves the major beta sheet surface of each of their respective adhesion domains . ^^^ The CD 58 binding site on human CD 2 was recently shown by nuclear magnetic resonance structural data in conjunction with site directed mutagenesis to be a highly charged surface area covering approximately 770A2 on the major AGFCC ' C ' ' face of the CD 2 immunoglobulin like ( Ig like ) NH 2 terminal domain . ^^^ Here we have identified the other binding surface of the CD 2 CD58 adhesion pair by mutating charged residues shared among CD 2 ligands ( human CD 58 , sheep CD 58 , and human CD 48 ) that are predicted to be solvent exposed on a molecular model of the Ig like adhesion domain of human CD 58 . ^^^ Thus , the interaction between CD 2 and CD 58 involves the major beta sheet surface of each adhesion domain . ^^^ Strict conservation of human and sheep CD 58 residues within the involved C and C ' strands and CC ' loop suggests that this region is particularly important for stable formation of the CD 2 CD58 complex . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
HIV infection of monocytes resulted in twofold elevation of adhesion molecule LFA 1 ( both alpha L / CD11a and beta 2 / CD18 subunits ) and LFA 3 ( CD 58 ) , with no apparent increase in LFA 2 ( CD 2 ) or various beta 1 integrins . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Leukocyte functional antigen 3 ( LFA 3 ) / cluster of differentiation 58 ( CD 58 ) antigen is known as a ligand for CD 2 antigen , which is a specific surface marker of T lymphocytes . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
In this report , we demonstrate that these anti bodies recognize CD 2 , and we show how antibodies directed toward either the adhesion domain of CD 2 or its ligand , CD 58 , specifically inhibit IL 12 induced proliferation and IFN gamma production by phytohemagglutinin activated T cells , leaving the response to IL 12 unaffected . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Analysis of several R CR pairs ( CD 2 CD58 , CD45RO CD 22 , CD 5 CD72 , CD11a CD 54 , gp 39 CD40 , and CD 28 CD80 ) demonstrated that comparable levels of expression of the Rs ( CD 2 , CD45RO , CD 5 , and CD 28 ) and of some of the CRs ( CD 58 , CD 22 , and CD 72 ) were in all cell lines ; however , CD 54 , CD 40 , and CD 80 were expressed constitutively on the HTLV 1 and HTLV 2 infected cell lines . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Blocking antibodies against leukocyte function associated antigen LFA 2 ( CD 2 ) , LFA 3 ( CD 58 ) , and Mac 1 ( CD11b / CD18 ) inhibit in vitro aggregate formation . ^^^ Cell adhesion molecules like CD 2 , CD 58 , and CD11b / CD18 may be involved in granuloma formation of Crohn ' s disease . . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The CD 2 molecule plays an important role in T cell adhesion by interacting with the ligands CD 58 ( LFA 3 ) and CD 59 . ^^^ Failure of multimeric rCD 2 to interact with the other cells could not be explained by differences in CD 58 expression , suggesting that , in terms of CD 2 binding , there are qualitative differences in CD 58 on different cell types . ^^^ Binding of multimeric rCD 2 to six of the seven reactive cells was virtually totally inhibited by CD 58 mAb pretreatment , whereas binding to the erythroleukemic line K 562 was only partially blocked , suggesting the existence of another CD 2 ligand . ^^^ Subsequent studies demonstrated that the putative new ligand is not CD 59 , and that it interacts with a different region of the CD 2 molecule than CD 58 , probably a site located between the T 11 ( 1 ) and T 11 ( 2 ) epitopes . ^^^ The binding affinity of CD 2 for the new ligand is 10 fold lower than for CD 58 and , based on studies with truncated rCD 2 , the binding site for the new ligand is located within the amino terminal 105 amino acids of the CD 2 polypeptide . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
All cell lines from MS and controls expressed increased levels of LFA 1 ( CD11a ) , LFA 2 ( CD 2 ) , LFA 3 ( CD 58 ) , ICAM 1 ( CD 54 ) , and VLA 4 ( CDw49d ) . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
CD11a and CD 2 were not detected on melanoma cells while CD 54 and CD 58 were coexpressed on the majority of the melanoma cell populations investigated . ^^^ CD 54 showed a higher degree of intra and intertumor heterogeneity than CD 58 . gamma Interferon and / or tumor necrosis factor alpha upregulated the expression of CD 54 by melanoma cells , but neither modulated that of CD 58 nor induced that of CD11a and CD 2 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Proliferation is also inhibited by mAbs to T cell CD 2 and LFA 1 beta chain ( CD 18 ) and by mAbs to LFA 3 ( CD 58 ) and CD 44 , which are expressed by T cells and EC . mAb to ICAM 1 ( CD 54 , a ligand for LFA 1 ) provides inconsistent inhibition , and mAb to ICAM 2 , used with or without anti ICAM 1 , is not inhibitory . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Thirteen patients were investigated using the following monoclonal antibodies ( mAbs ) : CD 2 , LFA 3 ( CD 58 ) , ICAM 1 ( CD 54 ) , LFA 1 ( CD11a ) , the alpha / beta and gamma / delta T cell receptor , pan T cells and broadly distributed monocyte / macrophage ( m / m phi ) [ Fc gamma RII ( CD 32 ) , 25F9 , RM3 / 1 ] . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Adhesion molecules such as CD 2 and its ligand CD 58 ( LFA 3 ) , as well as CD11a / 18 ( LFA 1 ) and CD 54 ( ICAM 1 ) regulate not only cell to cell attachment but also participate in lymphocyte activation , recirculation , and effector function including cytolytic activity towards tumor cells . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Additional mAb blocking studies revealed that CD 48 interacts with the T 11 ( 1 ) region of CD 2 , the same region of CD 2 that binds LFA 3 ( CD 58 ) . ^^^ Thus , CD 48 and CD 58 represent alternative and possibly competing ligands for CD 2 , although based on blocking studies with soluble CD 2 , CD 48 interacts with CD 2 with approximately a 100 fold lower affinity that CD58 . . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The induction of the initial proliferative wave of immature cortical thymocytes is carried out by the LFA 3 ( CD 58 ) adherence molecules , the receptors of CD 2 antigens located on reticulo epithelial cells . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Surface expression of the beta 2 integrin chains ( CD11a , CD11b , CD11c and CD 18 ) , and the beta 1 integrin chains ( CD49b , CD49d , CD49f and CD 29 ) , as well as that of members of the immunoglobulin superfamily ( CD 2 , CD 54 , CD 56 and CD 58 ) , were analyzed by one or two color flow cytometry . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
By virtue of sequence similarity , the genes encoding CD 2 , CD 48 , CD 58 , and Ly 9 have been assigned to a distinct subset within the immunoglobulin superfamily . ^^^ Previous gene mapping studies in human and mouse have suggested that CD 2 , CD 48 , and CD 58 arose by gene duplication . ^^^ The proximity in human and mouse genomes of the genes encoding CD 2 , CD 58 , and the Na , K ATPase alpha 1 subunit , and of the Ly 9 , CD 48 , and the Na , K ATPase alpha 2 subunit genes may be explained by the occurrence of two , successive duplication events during vertebrate evolution , and suggest that Ly 9 may also participate in adhesion reactions between T lymphocytes and accessory cells by homophilic interaction . . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The amino terminal domain of human CD 2 is responsible for cell adhesion , binding to CD 58 on antigen presenting cells or target cells . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Furthermore , the avidity of CD 2 for one ligand , CD 58 ( LFA 3 ) , is regulated by TCR signaling . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
However , IL 2 was a more potent activator of these functions , which have been shown to be partly mediated by CD 2 , CD 58 , beta 2 integrins , and ICAM 1 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The binding of Abs directed against other surface markers present on human T cells CD 2 , CD 3 , CD 5 , CD 8 , CD11A , CD 25 , CD45RO , CD45RA , CD 58 , TCR alpha , TCR gamma , and HLA DQ was not inhibited by gp 63 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The lateral redistribution of CD 2 and its ligand CD 58 ( LFA 3 ) in T cell and target membranes , respectively , has also been shown to affect cellular adhesion strength . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
CD 2 , CD 48 and CD 58 are closely related members of the immunoglobulin superfamily and their extracellular regions are predicted to have very similar structures . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Whereas CD 2 was not expressed at the time of initiation of thymic lymphopoiesis , a second CD 58 ligand , CD 48 , was expressed at 8 . 2 wk , suggesting a role for CD 48 early in thymic development . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Domain 1 of human CD 2 is responsible for cell adhesion , binding to CD 58 ( LFA 3 ) expressed on the cell to which the T cell binds . ^^^ Human CD 2 domain 1 requires N linked carbohydrate to maintain its native conformation and ability to bind CD 58 . ^^^ The model provides an explanation for the observed instability of deglycosylated human CD 2 , and allows residues that are important for CD 58 binding to be differentiated from those affecting conformational stability via interactions with the glycan . . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
SEB induced proliferation of T cells was inhibited by monoclonal antibodies to CD 2 , CD11a , CD 18 , CD 28 , CD 44 , CD 58 and ICAM 1 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Surface expression of the beta 2 integrin chains ( CD 18 , CD11a , CD11b , CD11c ) , as well as that of members of the immunoglobulin superfamily ( CD 2 , CD 54 , CD 56 , CD 58 ) , were analysed by one or two colour flow cytometry . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The eight AML M4Eo cases not only expressed the CD 2 antigen , but also its ligand CD 58 ( leukocyte function antigen 3 ) . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
CD 2 , CD 48 and CD 58 are structurally similar cell adhesion molecules forming a subset of the immunoglobulin superfamily ( IgSF ) . ^^^ In humans CD 58 is a ligand for CD 2 while in mice CD 2 binds CD 48 . ^^^ The upper limit of the affinity of the rat CD 48 CD2 interaction is 4 10 10 ( 5 ) M 1 , lower than the published affinity of human CD 2 for CD 58 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Human CD 2 , a glycosylated transmembrane receptor found on all T lymphocytes , plays a key role in facilitating cellular adhesion between T cells and target cells or antigen presenting cells by binding to its counter receptor CD 58 ( LFA 3 ) present on the surface of those cells . ^^^ All CD 2 adhesion functions are localized within the amino terminal 105 residue domain , which contains a single high mannose N glycan required for maintaining both the conformational stability and CD 58 binding properties of the glycoprotein . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Cellular adhesion molecules ( CAM ) , CD11a c , CD 18 , CD 54 , CD 56 , CD 58 and CD 2 ( T 11 ( 1 ) epitope ) , are up regulated in LAK cells by culture with IL 2 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The leukocyte function associated ( LFA ) adhesion molecules LFA 1 ( CD11a / CD18 ) , LFA 2 ( CD 2 ) , and LFA 3 ( CD 58 ) could not be detected in the cytoplasm or on the surface . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The study included beta 1 , beta 2 and beta 3 integrins , CD 2 , CD 54 and CD 58 ( belonging to the immunoglobulin superfamily ) , and CD 44 and L selectin ( homing receptors ) . alpha 1 and alpha 2 of the beta 1 integrins were non existent and alpha 3 was weak on freshly isolated NK cells , but their expression increased after 4 weeks in culture with IL 2 . ^^^ CD 2 , CD 44 , CD 54 and CD 58 were increased by IL 2 but L selectin was strongly down regulated on the long term activated NK cells . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
We find that LFA 3 ( CD 58 ) , the ligand for CD 2 is of predominant importance in predicting susceptibility of neuroblastoma to the cytotoxic actions of NK and LAK cells , while expression of ICAM 1 ( CD 54 ) may also modify susceptibility . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Our data also showed that T cells do not discriminate between peripheral tissues and skin or lymph nodes on the basis of virgin or memory CD45R phenotype , or CD 2 , CD 58 or CD 44 phenotype , and with the possible exception of CD11a / CD18 , that it is not mandatory for lymphocytes to be activated to adhesion moleculehi status in order to home to fetal skin . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The ligand for the T cell antigen CD 2 is CD 48 in rodents , but CD 58 in humans . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NKL cells express CD 2 , CD 6 , CD11a , CD 26 , CD 27 , CD 29 , CD 38 , CD 43 , CD 58 , CD 81 , CD 94 , CD 95 , class 2 MHC , and the C1 . 7 . 1 antigen , but do not express detectable levels of CD 3 , CD 4 , CD 5 , CD 8 , CD 14 , CD 19 , CD 20 , CD 28 , alpha / beta or gamma / delta T cell receptors on the cell surface . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The T cell and NK cell adhesion molecule CD 2 interacts with different ligands , viz , CD 58 , CD 48 , and CD 59 . ^^^ CD2L is restricted in expression to myeloid cells , where it is co expressed with CD 58 on monocytes and is the dominant , if not sole , CD 2 ligand on neutrophils . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The natural ligand for CD 2 is CD 58 , but controversy exists over alternative or additional ligands that could deliver the second signal in vivo . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
This may be due to the low expression of ligands ( CD 54 , CD 58 , CD 80 ) for the T cell accessory receptors CD11 / 18 , CD 2 and CD28 / CTLA 4 on mucosal macrophages . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The ligands of murine and human CD 2 are CD 48 and CD 58 , respectively , and interactions between these molecules have been shown to influence antigen recognition and T cell activation . ^^^ The CD 58 binding site of human CD 2 has been characterized in mutational studies , and here we use heteronuclear NMR spectroscopy to identify the rat CD 48 binding site of the N terminal domain of rat CD 2 ( CD2d1 ) . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Infiltrating mononuclear cells expressed CD11a ( 68 % ) , CD 18 ( 73 % ) , CD11b ( 13 % ) , CD11c ( 21 % ) , CD 58 ( 13 % ) , CD 4 ( 44 % ) , CD 8 ( 17 % ) , CD62L ( L selectin ) ( 18 % ) , CD49d ( 38 % ) , CD49e ( 15 % ) , CD 2 ( 56 % ) , and CD 44 ( 77 % ) . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Antigen independent maturation of CD 2 , CD11a / CD18 , CD 44 , and CD 58 expression on thymic emigrants in fetal and postnatal sheep . ^^^ We have compared the expression of CD 2 , CD11a / CD18 , CD 44 , and CD 58 and alpha beta and gamma delta T cells emigrating from the fetal and postnatal thymus . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The pan T cell marker CD 2 and its ligand CD 58 mediate these immune function . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
CD 2 mediates interaction between T cells and their cognate partners through its CD 58 binding membrane distal adhesion domain ( D 1 ) facilitating T cell receptor ( TCR ) triggering . ^^^ Co ligation of CD 2 and CD 58 molecules on opposing cells within a conjugate pair induces CD2R and redistributes CD 2 to the region of cell cell contact . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Intact CD 43 mAb 6F5 , in contrast to monovalent Fab fragments , enhanced T cell adhesiveness by transactivating CD 2 binding to CD 58 molecules . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The autologous MECLR was inhibited in samples from all patients by mAb to CD 2 ( LFA 2 ) , CD11a ( LFA 1alpha ) , CD 18 ( LFA 1beta ) , ICAM 1 , CD 58 ( LFA 3 ) and an antiserum to IL 1beta . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
CD 2 CD 58 interaction and the control of T cell interleukin 12 responsiveness . ^^^ Antibodies specific for the adhesion domain of either CD 2 or its primary ligand , CD 58 , inhibited the major functional responses of T cells to IL 12 without affecting the response to IL 2 . ^^^ The CD 2 CD58 interaction between activated T cells and monocytes was found to be a critical factor in the modulation of T cell IL 12 responsiveness , and experiments using CD 58 transfected Chinese hamster ovary ( CHO ) cells , and stimulatory pairs of CD 2 antibodies confirmed the key role of CD 2 ligation in optimizing T cell IL 12 signaling . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
In contrast , the ligand to the CD 2 , the CD 58 marker , was rarely detected . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Chimeric proteins in which the CD 2 transmembrane and / or cytoplasmic domains were deleted or exchanged with analogous regions of CD 4 , CD 28 or CD 58 retained association with high levels of murine CD 45 phosphatase activity , suggesting that the CD 2 extracellular domain largely controls interaction with CD 45 . ^^^ To a lesser extent , the cytoplasmic domain of CD 2 was also shown to interact with CD 45 , as demonstrated by an increase in co immunoprecipitated phosphatase activity observed following replacement of the CD 58 cytoplasmic domain with that of CD 2 . ^^^ Deletion of the CD 2 cytoplasmic domain , excluding the first three amino acids , removed virtually all CD 2 associated zeta chain and approximately sevenfold higher levels of zeta chain were found in association with a CD58 / 58 / 2 chimera than with control human CD 58 wild type . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Nucleosomes impaired neither the conjugation step nor the expression of adhesion molecules at the effector ( CD11a , CD 18 , CD 2 ) or target ( CD 54 , CD 58 ) cell surface . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Surprisingly , we found a large contiguous patch of residues in the counterreceptor ( CD 58 ) binding site of human CD 2 exhibiting slow conformational exchange motions ( ms microsecond ) . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Reduced levels of a soluble form of the adhesion receptor and CD 2 ligand CD 58 ( sCD 58 ) were previously described in RA patients . ^^^ In order to understand the biological significance of this finding we biochemically characterized sCD 58 in RA and asked how well sCD 58 binds to CD 2 . sCD 58 concentrations were measured in serum and synovial fluid ( SF ) samples of RA patients by two ELISAs , one detecting domain 1 of CD 58 ( CD 58 D1 ) , and the other one the complete molecule ( CD 58 D1 + D 2 ) . ^^^ Binding of SF sCD 58 to the CD2+ Jurkat variant JBB 1 or recombinant CD 2 was stronger than urinary sCD 58 and reached binding of oligomeric recombinant CD 58 at low concentrations . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The immunoglobulin superfamily includes leukocyte function antigen 2 ( LFA 2 or CD 2 ) , leukocyte function antigen 3 ( LFA 3 or CD 58 ) , intercellular adhesion molecules ( ICAMs ) , vascular adhesion molecule 1 ( VCAM 1 ) , platelet endothelial cell adhesion molecule 1 ( PE CAM 1 ) , and mucosal addressin cell adhesion molecule 1 ( MAdCAM 1 ) . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
We have generated a chimeric receptor composed of the signaling domains of CD 28 and the extracellular domain of CD 2 which binds the widely expressed ligand CD 58 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
M2 , and have partially mapped the epitopes of these and other mitogenic CD 2 specific monoclonal antibodies by way of recognition of CD 2 : CD 58 chimeric proteins possessing either the N terminal or the membrane proximal immunoglobulin domains of CD 2 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
In rodents , CD 48 is the ligand for CD 2 whereas in humans , CD 58 is the ligand for CD 2 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
CD 48 , the murine homolog of human CD 58 , binds to CD 2 in rats and mice . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
A human skin allograft injury model in immunodeficient mice , engrafted with human peripheral blood mononuclear cells from a different donor , has been used to test whether reagents that block human T cell CD 2 interactions with its principal ligand , LFA 3 ( CD 58 ) , can inhibit immune reactions in vivo . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Exposure of either Raji or K 562 cells to mAb against CD 58 inhibited the transfer of CD 2 . ^^^ The present study indicates that following their activation , T cells gain the capacity to transfer T cell antigens to non T cells and that CD 2 and CD 58 molecules are involved in this process . . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
We investigated the contribution of CD 2 , CD 3 , CD11a / CD18 , CD 54 and CD 58 molecules in T lymphocyte tumor cell interactions mediated by chimeric immunoglobulin receptors . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
RESULTS : In situ immunophenotyping analysis of CAM provided evidence for the intrahepatic expression of leucocyte adhesion molecules ( CD11a and CD 2 ) and their corresponding ligands on hepatocytes ( CD 54 and CD 58 ) in all cases . ^^^ A significant parallel expression of CD11a and CD 54 as well as CD 2 and CD 58 structures , restricted to hepatic lobules within the disease sites , was also observed , even if their induction exhibited different degrees of correlation with biological and / or histological activities . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The interaction of the T cell glycoprotein CD 2 and its ligand CD 58 is important for T cell interaction with antigen presenting and target cells . ^^^ Interaction between T cells that express CD 2 and glass supported planar bilayers containing purified and fluorescently labeled CD 58 leads to accumulation of CD 58 ( fluorescence ) in the cell / bilayer contact area . ^^^ CD 58 molecules accumulated within the contact area in excess of the CD 58 density in the bilayer outside the contact area can be considered as bound by cell surface CD 2 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The extracellular Ig like domains presented structural and sequence homology with a group of members of the Ig superfamily that included CD 2 , CD 48 , CD 58 , and Ly 9 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Antibodies against the CD 58 ligand , CD 2 , partly inhibited the adhesion response in beta 2 integrin negative T cells whereas antibodies against other adhesion molecules did not . ^^^ The adhesion response in beta 2 integrin positive T cells was partly inhibited by antibodies against CD 58 and CD 2 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The crystallographic analysis of the CD 2 binding domain of CD 58 strongly supports this concept . . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Human b cell differentiation : dependence on interactions with monocytes and T lymphocytes via CD 40 , CD 80 ( B7 . 1 ) , and the CD 2 Ligands CD 48 and CD 58 ( LFA 3 ) . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The CD 48 molecule belongs to a subfamily of the Ig superfamily that also includes the CD 2 , CD 58 , 2B4 , Signaling lymphocyte activation molecule ( SLAM ) , and Ly 9 molecules . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
T lymphocyte activation through the T cell receptor ( TCR ) / CD3 complex alters the avidity of the cell surface adhesion receptor CD 2 for its ligand CD 58 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The new structural information supports a ' hand shake ' model of CD 2 CD58 interaction involving the GFCC ' C ' ' faces of both CD 2 and CD 58 adhesion domains . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Unlike many other CD 2 Abs , M 1 and M 2 do not interfere with TCR / CD3 triggering nor do they inhibit binding of CD 2 to its ligand CD 58 , thus preserving the physiological functions of these important effector cell molecules . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The preparation of a soluble chimeric form of CD 58 , the ligand of the human T cell surface recognition molecule CD 2 , illustrates the usefulness of the combined approach when expression levels are low or the deglycosylated protein is unstable at low pH . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Flow cytometric analysis demonstrates that mPEG modification dramatically decreases antibody recognition of multiple molecules involved in essential cell : cell interactions , including both T cell molecules ( CD 2 , CD 3 , CD 4 , CD 8 , CD 28 , CD11a , CD62L ) and antigen presenting cell ( APC ) molecules ( CD 80 , CD 58 , CD62L ) likely preventing the initial adhesion and costimulatory events necessary for immune recognition and response . . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
While blocking CD 2 slightly decreased cytotoxicity , contrary to expectations , an antibody against CD 58 ( the ligand for CD 2 ) , failed to block killing and instead mediated an increased cytotoxicity that correlated with target density of CD 58 . ^^^ The CD 58 antibody enhanced killing was dependent not only on FcRgammaIII but also on CD 2 and ICAM 1 / CD18 . ^^^ Unlike CD 58 antibody mediated ADCC , Herceptin ADCC was minimally affected by blocking antibodies to CD 2 or ICAM 1 / CD18 , which suggests a different mechanism of action . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
CD 2 serves as both an adhesion molecule and as a costimulatory molecule through interactions with its ligand , CD 58 , on antigen presenting or target cells . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
All expressed CD 2 , CD 4 , CD 58 , CD 69 and CD 80 and therefore resembled activated T cells . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Moreover , the former DC subpopulation clearly showed higher expression of the CD 13 myeloid associated antigen , the CD 29 and CD 58 adhesion molecules , the CD 2 , CD 5 and CD 86 costimulatory molecules , the CD 32 IgG receptor and the CD11c complement receptor . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
B4 : an NK cell activating receptor with unique specificity and signal transduction mechanism . 2B4 is a cell surface glycoprotein of the Ig superfamily structurally related to CD 2 like molecules such as CD 2 , CD 48 , CD 58 , CD 84 , Ly 9 , and SLAM . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Lymphoma cells in cases with angiodestruction frequently expressed CAMs CD 2 , CD11a , and CD49d and their ligands CD 58 , CD 54 , and CD 106 and were positive for CD 122 and cytotoxic granule proteins TIA 1 , perforin , and granzyme B . ^^^ Lymphoma cells in cases without angiodestruction mostly were negative for CD 2 , CD 58 , CD 54 , CD 106 , and TIA 1 and weakly positive for perforin and granzyme B . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
While H . saimiri transformed T cells resemble native T cells in numerous aspects , their reactivity to CD 2 is strikingly different : Native T cells are activated via CD 2 by certain pairs of mAbs , but not by the mere binding of CD 2 to its ligand CD 58 . ^^^ In contrast , H . saimiri transformed T cells are activated by a single crosslinked anti CD 2 mAb and also by interaction with CD 58 bearing cells . . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
A space time structure determination of human CD 2 reveals the CD 58 binding mode . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Members of this family are CD 2 , CD 48 , CD 58 , CD 84 , signaling lymphocytic activation molecule ( SLAM ) , 2B4 and Ly 9 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The ligand binding surface of the T lymphocyte glycoprotein CD 2 has an unusually high proportion of charged residues , and ionic interactions are thought to play a significant role in defining the ligand specificity and binding affinity of CD 2 with the structurally homologous ligands CD 48 ( in rodents ) and CD 58 ( in humans ) . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
In addition , we analysed ( by flow cytometry ) whether this function correlated with modifications of the CD 2 , CD11a , CD11b and CD 18 molecules , which are involved in the adhesion of effector cells to the counter receptors ( CD 54 and CD 58 ) on osteosarcomas . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Ly 9 consists of four Ig like domains with the structural features of the CD 2 subfamily , which includes CD 2 , CD 48 , CD 58 , 2B4 , CD 84 , and CDw15O ( SLAM ) . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
IEL killing of C1R cells involved interaction of major histocompatibility complex ( MHC ) class 2 with TCR , CD 2 with CD 58 , and CD11a with CD 54 , and was perforin mediated . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
CD 58 is the ligand for the CD 2 molecule on human T cells and has been shown to provide a co stimulatory signal for T cell activation . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Other members of this family include CD 2 , CD 48 , CD 58 , CD 84 , signaling lymphocytic activation molecule and Ly 9 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : T cells were treated with anti CD 2 antibodies or cells bearing the natural CD 2 ligand CD 58 , after which signaling through STAT transcription factors was assessed . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The responses to lactoglobulin and PHA were inhibited by the CTLA4 / Fc chimera and by MAbs against CD 2 , CD 58 , CD 80 , and CD 86 , indicating stimulation of CD28+ LPLs with antigen presenting cells through activation of the CD 2 pathway . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Here we show that a significant fraction of human CD 2 molecules is inducibly recruited into lipid rafts upon CD 2 cross linking by a specific pair of mitogenic anti CD 2 monoclonal antibodies ( anti T 11 ( 2 ) + anti T 11 ( 3 ) ) or during cellular conjugate formation by CD 58 , the physiologic ligand expressed on antigen presenting cells . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Molecular dissection of the CD 2 CD58 counter receptor interface identifies CD 2 Tyr86 and CD 58 Lys34 residues as the functional `` hot spot ' ' . ^^^ The CD 2 CD58 crystal structure offers a detailed view of this key functional epitope : CD 2 D31 and D 32 orient the side chain of CD 58 K34 such that CD 2 Y86 makes hydrophobic contact with the extended aliphatic component of CD 58 K34 between CD 2 Y86 and CD 58 F46 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The CD 2 subset of the immunoglobulin superfamily consists of a rapidly expanding family of leukocyte cell surface receptors , at least five of which ( CD 2 , CD 48 , CD 58 , CD 150 , and CD 244 ) are involved in lymphocyte activation as either receptors or ligands . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
All MC species analyzed including HMC 1 were found to express LFA 3 ( CD 58 ) , the natural ligand of CD 2 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
This study showed that presentation of SEA by RPE cells to resting T cells was dependent on the presence of the molecules CD 2 , CD 58 and CD 18 , CD 54 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The performance of the algorithm is demonstrated by docking three complexes of immunoglobulin superfamily domains , CD 2 to CD 58 , the 5 ( alpha ) domain of a T cell receptor to its 5 ( beta ) domain , and a T cell receptor to a pMHC complex as well as a small molecule inhibitor to a phosphatase . . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
This set of genes , related to CD 2 and CD 58 on Chromosome ( Chr ) 1p98 , are found clustered close together in the human genome on Chr 1q22 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Several amino acids ( aa ) important for ligand interaction , molecular folding or signal transduction , found in other mammalian CD 2 , were found to be highly conserved in the predicted fCD 2 aa sequence . fCD 2 expressing cells were able to form rosettes with human red blood cells ( probably via human CD 58 ) , and the rosette formation was inhibited by an anti fCD 2 monoclonal antibody . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
MATERIAL / METHODS : 140 EMBs from dilated cardiomyopathy ( DCM ) patients and 14 autopsy heart samples ( controls ) were immunostained for T lymphocytes ( CD 2 , CD 3 , CD 4 , CD 8 ) , beta ( 2 ) integrin+ infiltrates ( CD 18 , LFA 1 , Mac 1 ) and CAMs ( immunoglobulin superfamily : ICAM 1 , HLA class 1 , HLA DR , VCAM 1 , CD 58 ; selectins : CD62E and CD62P ; and the beta ( 1 ) integrin chain CD 29 ) . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Dynamic binding between CD 2 and CD 58 counter receptors on opposing cells optimizes immune recognition through stabilization of cell cell contact and juxtaposition of surface membranes at a distance suitable for T cell receptor ligand interaction . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
In order to establish the mechanism ( s ) of immunomodulatory and anti tumor properties of sheep erythrocytes ( SRBC ) , the T 11 target structure ( T11TS ) or CD 58 molecule as a pertinent component of SRBC was isolated , purified and finally administered in rats with experimentally induced brain tumor . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The importance of cell surface molecules was examined by application of a panel of blocking antibodies ( CD 2 , CD 18 , CD 40 , CD40L , CD 54 , CD 58 ) in addition to use of TCR negative T cell lines . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Fluorescence activated cell sorting analyses showed that neither tumors nor ascitic cells grown in SCID mice expressed leukocyte function associated antigen ( LFA ) 1alpha ( CD11a ) , LFA 1lbeta ( CD 18 ) , LFA 2 ( CD 2 ) , LFA 3 ( CD 58 ) , intercellular adhesion molecule ( ICAM ) 1 ( CD 54 ) , ICAM 2 ( CD 102 ) , ICAM 3 ( CD 50 ) , or leukocyte endothelial adhesion molecule ( LECAM ) 1 ( CD62L ) , suggesting that these cytoadhesion molecules are not involved in tumor formation of RM P 1 cells in vivo . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
A number of general principles can be derived from the structural and functional studies of the extracellular domains of CD 2 and CD 58 and their complex . ^^^ Significant electrostatic interactions within the protein protein interfaces contribute directly to the formation of macromolecular complexes of CD 2 and CD 58 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The forced detachment of CD 2 and CD 58 shows that the system can respond to an external force by two mechanisms , which depend on the loading rate . ^^^ These findings suggest that protein unfolding is not coupled to the adhesive function of CD 2 and CD 58 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
CD 2 is known to mediate the activation of T and natural killer ( NK ) cells through its interaction with CD 58 . ^^^ Here we show that CD 2 engagement significantly increases class 2 , costimulatory ( CD 40 , CD 80 , CD 86 ) , adhesion ( CD 54 , CD 58 ) , and CCR 7 molecule expression on primary dendritic cells . ^^^ Lastly , interference with dendritic cell CD 2 T cell CD 58 engagement decreases naive CD4+CD45RA+ T cell proliferation . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Porcine CD 58 : cDNA cloning and molecular dissection of the porcine CD 58 human CD 2 interface . ^^^ Of the two main candidates , i . e . , CD 48 and CD 58 , the cDNA of the most likely ligand poCD 58 was cloned from CD 48 negative endothelial cells costimulating human CD 4 ( + ) T cells through the CD 2 pathway . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
CD 2 ( cluster of differentiation 2 ) is a cell adhesion molecule expressed on T cells and is recognized as a target for CD 48 ( rats ) and CD 58 ( humans ) . ^^^ The functional `` hot spot ' ' for the adhesion surface of CD 2 and CD 58 has been dissected . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The immunoprofile of the cells was concordant with that of MM cells : positive for CD 10 , CD 28 , CD 38 , CD 138 , CD 56 , CD49d , CD 44 , CD 54 and CD 58 , negative for CD 19 , CD 40 , CD 95 , CD95L , CD 34 , CD 2 and CD 5 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
BACKGROUND : Human CD 58 is an adhesion molecule that interacts with CD 2 on lymphocytes . ^^^ CONCLUSIONS : The CD 2 interaction with porcine cells is important for the efficient recognition of porcine tissue , and inhibition of the human antiporcine immune response with the antibody is likely to be caused by the disruption of the human CD 2 interaction with this porcine homologue of CD 58 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The interaction between cell adhesion molecules CD 2 and CD 58 is critical for an immune response . ^^^ Docking studies of CD 2 peptides and CD 58 protein revealed the possible binding sites of the cyclic peptides on CD 58 protein . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
A panel of anti human CD 2 monoclonal antibodies ( mAb ) and soluble human CD 58 ( LFA 3 ) were tested for binding to human peripheral blood mononuclear cells ( PBMCs ) , recombinant human CD 2 and mononuclear cells from Cynomolgus , Rhesus and African green monkey , Stump tail , Pig tail and Assamese macaque , Chimpanzee and Baboon . ^^^ Furthermore , the analysis of the competition of several of the anti human CD 2 antibodies with each other and CD 58 demonstrated the existence of a continuum of overlapping epitopes on human CD 2 , which is in contrast to the commonly held belief that epitopes on human CD 2 are clearly segregated . . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The immunoglobulin CD 2 is a cell adhesion molecule that mediates T cell activation by binding to its receptor CD 58 on antigen presenting cells ( APCs ) . ^^^ The CD 2 expression on the surface of Jurkat cells and the CD 58 expression on the Caco 2 cells were confirmed by flow cytometry and ELISA studies , respectively . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Cell surface receptors belonging to the CD 2 subset of the Ig superfamily of molecules include CD 2 , CD 48 , CD 58 , 2B4 , signaling lymphocytic activation molecule ( SLAM ) , Ly 9 , CD 84 , and the recently identified molecules NTB A / Ly108 / SLAM family ( SF ) 2000 , CD84H 1 / SF2001 , B lymphocyte activator macrophage expressed ( BLAME ) , and CRACC ( CD 2 like receptor activating cytotoxic cells ) / CS 1 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Yet how low affinity adhesion molecules such as CD 2 and CD 58 can produce stable cell adhesion is still not completely understood . ^^^ In this paper , we present a theoretical model that simulates the accumulation of CD 2 and CD 58 in the contact area of a Jurkat T lymphoblast and a CD 58 containing substrate . ^^^ Mobile CD 2 and CD 58 can diffuse freely on both the cell and substrate . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The interaction between cell adhesion molecules CD 2 and CD 58 is critical for immune response . ^^^ The 12 amino acid synthetic cyclic peptides effectively blocked the interaction between CD 2 and CD 58 proteins as demonstrated by E rosetting and heterotypic adhesion assays . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
LPLs preferentially recognized CD 58 rather than the major histocompatibility complex molecules on monocytes , triggering proliferation and interferon gamma ( IFN gamma ) secretion that was inhibited by blocking the CD 2 CD58 interaction . ^^^ This study shows that LPLs are constitutively activated through CD 2 , that they preferentially recognize CD 58 on monocytes and that CD 2 stimulation leads to CD 3 hyporesponsiveness . . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
We found that the diminished mouse helper T cell responses to xenoantigens result from at least two defects in cell surface molecule interactions between T cells and xenogeneic APCs , specifically TCR and / or CD 8 interactions with xenogeneic class 1 MHC molecules and accessory molecule interactions with their ligands ( probably LFA 1 with ICAM 1 / ICAM 2 and / or LFA 2 with LFA 3 ) . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Furthermore , LZ 8 increased the expression of CD 2 , but not VLA 4 , VLA 5 or LFA 3 , on MOLT 4 and enhanced rosette formation between human T cells and sheep red blood cells . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Ligands to three of these molecules have been identified ; ICAM 1 , LFA 3 , and hyaluronic acid binding to LFA 1 , LFA 2 , and CD 44 , respectively . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The K 562 plus SEB induced response can be inhibited by combinations of mAbs to CD 2 and LFA 1 , and to LFA 3 , ICAM 1 , and B 7 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
In addition eosinophils express 2B4 , CD 84 , CD 58 , and CD 48 , but not signaling lymphocytic activation molecule or CD 2 , on their surface ( FACS ) . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
AnTMSA 2 is a CD 4 T lymphocyte expressing high levels of MHC class 2 molecules , CD 58 and CD 2 , which are important for proliferation and growth . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Intercellular adhesion molecule type 1 ( ICAM 1 ) , lymphocyte function associated antigen type 1 ( LFA 1 ) , LFA 3 , CD 2 , and CD 44 expression were determined using indirect immunofluorescence techniques . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
This effect was not reproduced when DR 1 and LFA 3 were expressed by separate populations of DAP . 3 cells , suggesting that the ligands for CD 2 and for the T cell ' s receptor must be expressed on the same cell membrane . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Using anti LFA 3 monoclonal antibody ( mAb ) treated , interleukin 2 ( IL 2 ) cultured peripheral blood lymphocytes ( PBL ) or cloned CD3+ / CD8+ cells as lymphocyte activated killer ( LAK ) effectors , and ligand ( CD 2 ) negative tumor cell lines as targets , a down regulation of CD 3 and CD3+ cell mediated LAK activity was consistently observed . ^^^ The inhibitory effect of anti LFA 3 mAb was not prevented by stimulatory anti CD 2 mAb . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
By contrast , antibodies specific for LFA 3 or its ligand CD 2 only significantly blocked PBL adhesion to renal cells which had been pretreated with TNF alpha . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Although the ligand for human CD 2 has been defined as LFA 3 , that for murine CD 2 has not been identified yet . ^^^ The putative mouse CD 2 ligand recognized by this mAb was a glycosyl phosphatidylinositol anchored glycoprotein with an apparent molecular mass of 45 kD , which were shared characteristics with human LFA 3 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Rather , the CD 2 ( LFA 2 ) interaction with LFA 3 expressed by enterocytes may serve both to anchor IEL and to provide an accessory stimulus for activation . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The sequence data also support the view that Ly 9 belongs to the subgroup of the Ig superfamily that includes Bcm 1 , CD 2 , and LFA 3 . . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The infiltrating cells expressed LFA 1 and CD 2 , the correlating ligands for ICAM 1 and LFA 3 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The CD 2 receptor on T lymphocytes plays a major part in mediating adhesive interactions via the LFA 3 ligand and in transducing signals for lymphocyte activation . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
In inflammatory liver diseases , portal tract and periportal and intralobular areas of inflammation consisted virtually only of CD45RO positive ( UCHL 1 positive ) memory T cells , which strongly expressed the CDw 29 ( 4B4 ) antigen , and the adhesion molecules LFA 1 , CD 2 , LFA 3 , CD 44 and VLA 4 and the activation marker human leukocyte antigen DR . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The cell adhesion molecule , LFA 3 , is a natural co ligand for CD 2 . ^^^ NK cell supernatants were harvested after 3 4 d of incubation with 0 100 U / ml IL 2 , or after incubation with anti CD 2 ( T 11 ( 3 ] MAb and sheep red blood cells ( SRBC are a homologue for LFA 3 ) . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
In the present study , sequential changes in the expression of immune adhesion receptors ( LFA 1 , CD 2 ) and their ligand molecules ( ICAM 1 , LFA 3 ) were studied in heart allografts during rejection . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Multimeric binding of CD 2 on T cells to its physiologic ligand LFA 3 on cognate partner cells in vitro efficiently augments the antigen specific T cell signal delivered by the T cell receptor / CD3 complex . ^^^ The precise contribution of the antigen nonspecific CD 2 LFA 3 interactions to T cell immune responses in vivo , however , has been difficult to assess . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Of the remaining antibodies that were tested , none of those against CD 2 , LFA 3 , Mac 1 , p 150 , 95 , CD 43 , CD45RA or CD 56 affected binding . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
As with antibodies to LFA 3 and CD 2 , antibodies to LFA 1 and ICAM 1 also inhibited PHA induced mature thymocyte activation when accessory signals were provided by TE cells in vitro . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
We have shown that human thymic epithelial ( TE ) cells produce IL 1 alpha , IL 1 beta , and TE cells bind to thymocytes by CD 2 and LFA 1 molecules on thymocytes and LFA 3 , ICAM 1 on TE cells . ^^^ Inasmuch as the interaction between thymocytes and TE cells is mediated in part by CD 2 molecules on thymocytes and LFA 3 molecules on TE cells we next determined the effect on IL 1 release of ligand binding ( anti LFA 3 mAb TS2 / 9 ) to TE cell surface LFA 3 . ^^^ The identification of LFA 3 , the cell surface ligand for thymocyte CD 2 molecules , as a molecule via which TE cell derived cytokine production may be regulated suggests a mechanism at the cell surface by which direct TE cell thymocyte interaction might result in the triggering of local IL 1 release within the human thymic microenvironment . . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The mouse BCM 1 ( OX 45 , Blast 1 ) antigen has been cDNA cloned and sequenced to provide data supporting the view that BCM 1 , LFA 3 , and CD 2 constitute a subgroup within the Ig superfamily . ^^^ A similar situation is seen in the region of the CD 2 and LFA 3 genes between mouse chromosome 3 and human chromosome 1p . ^^^ Taken together , the data suggest duplication of a chromosome region including the precursors of the genes for BCM 1 , CD 2 , and LFA 3 , and the ATPase genes to give rise to the linkage groups now observed . ^^^ CD 2 and LFA 3 are more dissimilar in sequence than BCM 1 and LFA 3 , and if the precursors of the CD 2 and LFA 3 loci formed before the proposed chromosome segment duplication , then a gene encoding a recognizer molecule for BCM 1 may exist in linkage with Bcm 1 / BCM1 on chromosome 1 ( mouse ) and 1q ( human ) . . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
In addition , TNF alpha was released when monocyte LFA 3 bound immobilized , purified CD 2 , which is its physiologic receptor . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
T T cell activation required cell contact , was not major histocompatibility complex ( MHC ) restricted and was blocked by monoclonal antibodies directed against adhesion molecules CD 2 and LFA 3 but was not blocked by antibody to class 2 MHC determinants . ^^^ As CD 2 is the natural ligand for LFA 3 , increased expression of T cell surface adhesion molecules LFA 1 , ICAM 1 and particularly LFA 3 during an inflammatory response may rapidly recruit T cells that are activated through the CD 2 pathway . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Hamster fibroblasts transfected with murine CD 2 cDNA exhibited increased conjugate formation with a murine mastocytoma P 815 which expresses the putative murine LFA 3 mRNA detected by cross hybridization with human LFA 3 cDNA under conditions of low stringency . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
LFA 3 , a ligand for CD 2 , but not ICAM 1 , a ligand for LFA 1 , was , expressed on glioma cells . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The important role of LFA 1 in T cell blood DC adhesion was also supported by the metabolic energy and divalent cation dependence of the interaction . mAb against LFA 3 and CD 2 did not inhibit T cell blood DC binding . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Mouse LFA 3 studied with chimeric soluble CD 2 shows preferential expression on lymphoid cells . ^^^ CD 2 hC mu , to detect its ligand LFA 3 . ^^^ Since mouse LFA 3 is preferentially expressed on lymphoid cells and since CD 2 is expressed by both T and B lymphocytes , we would favor the view that the CD2 / LFA 3 adhesion system in the mouse may play a role in interactions between lymphocytes ( T T and / or T B ) rather than in cell interactions between lymphocytes and non lymphoid cells in the thymic , bone marrow or spleen microenvironments . . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
In addition , mAb to the adhesion molecules LFA 3 , CD 2 , LFA 1 , CD 29 , and to the tyrosine phosphatase CD 45 also inhibited proliferation , indicating the involvement of T to T cell interactions . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Adhesion mediated by interaction of CD 2 with LFA 3 is dramatically altered by surface charge and adhesion receptor density in such a way that this pathway is latent in resting T lymphocytes but becomes active over a period of hours following T cell activation . ^^^ CD 2 ligation can mediate or enhance T cell activation , suggesting that signals from CD2 / LFA 3 adhesive interactions are integrated with signals from the T cell antigen receptor during immunological recognition . ^^^ Interaction of LFA 1 with ICAMs is also regulated by cell activation but in a different way than in interaction of CD 2 with LFA 3 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Influence of receptor lateral mobility on adhesion strengthening between membranes containing LFA 3 and CD 2 . ^^^ Reduction of CD 2 mobility on Jurkat cells at 5 degrees C greatly decreased the rate of adhesion strengthening with the TM isoform of LFA 3 , resulting in a 30 fold difference between the two LFA 3 isoforms . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Large granular lymphocytes , mediators of NK activity , bind to other cells using both the LFA ( lymphocyte function associated ) 1 ICAM and the CD 2 LFA 3 adhesion pathways . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
A second family of adhesion receptors is the immunoglobulin superfamily , members of which include CD 2 , LFA 3 and ICAM 1 , which participate in T cell adhesive interactions , and the antigen specific receptors of T and B cells , CD 4 , CD 8 and the MHC Class 1 and 2 molecules . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Since the interaction thymic epithelial cells ( ICAM 1 and LFA 3 positive ) / thymocytes ( LFA 1 and CD 2 positive ) has been shown to be necessary for promotion of activation and maturation of thymocytes , the interaction keratinocytes ( ICAM 1 and LFA 3 positive , as we demonstrated ) / T lymphocytes ( LFA 1 and CD 2 positive as well ) might be , by analogy , important not only for the `` homing ' ' of T lymphocytes within the epidermis , but also for the epidermis being considered a peripheral inductive site for T cell activation and maturation . . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
HIV 1 infection of target monocytes or T cells by cell free virus was blocked completely or partially by some mAb that prevent cell cell interactions ( CD 4 , HLA DR , LFA 1 , LFA 3 ) , but not by others ( ICAM 1 , MAC 1 , gp150 . 95 , CD 2 , CD 3 , CD 14 ) . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
It is a member of the immunoglobulin superfamily , mediates cell adhesion , and has significant sequence homology to two other adhesion molecules , CD 2 and LFA 3 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
CD 2 delta S+ hybridomas were able to respond to Ag and to LFA 3 plus an anti CD 2 mAb . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
This interaction is mediated by binding of CD 2 to the broadly distributed surface polypeptide LFA 3 and augments the recognition function of the CD 3 Ti antigen major histocompatibility complex receptor via stabilization of conjugate formation between cells . ^^^ This amino terminal domain fragment binds to LFA 3 on human B cells with a dissociation constant of 0 . 4 microM , possesses functional immunoadhesion epitopes as defined by the binding of monoclonal antibodies raised to native CD 2 , and retains the ability to inhibit sheep erythrocyte rosette formation with human T cells . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Both types of endothelial cells possess several kinds of Cell Adhesion Molecules ( CAMs ) , such as ICAM 1 , CD 44 , LFA 3 , but not LFA 1 or CD 2 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Activation of lymphocytes by EC however was also antigen independent , and may be due to T cell ( CD 2 ) endothelial cell ( LFA 3 ) ligand interactions which are functional on EC but not RPE . . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Monoclonal antibodies ( MAb ) against classes 1 and 2 antigens of the major histocompatibility complex ( MHC ) and their receptors , CD 8 and CD 4 , had no effect on T cell interaction with SSc and normal cells , while MAb against lymphocyte function associated antigen type 3 ( LFA 3 ) and CD 2 both strongly inhibited lymphocyte attachment . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
In the adhesion reaction , CD 2 on T cells binds to LFA 3 on other cells , with binding through domain 1 of CD 2 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Naive ( unsensitized ) and memory ( antigen primed ) T cells can be phenotypically distinguished on the basis of the high or low intensity with which they express a number of immunologically relevant lymphocyte membrane antigens , including CD45R , CDw 29 , UCHL 1 , LFA 1 , LFA 3 , CD 2 and Pgp 1 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Two main molecular adhesion pathways have been characterized involving the CD 2 molecule binding the LFA 3 molecule and the LFA 1 molecule binding the ICAM 1 or ICAM 2 molecule . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Finally , other non organ specific lymphocyte adhesion molecules for EC may include CD 4 and CD 8 ( which bind to class 2 and class 1 MHC antigens , respectively ) , and CD 2 ( which binds to LFA 3 ) . . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The major pathway by which EC augment T cell IL 2 production is cell contact dependent and involves the CD 2 : LFA 3 ligand pair . ^^^ However , use of blocking mAb to CD 2 and LFA 3 , of PI LFA 3 , and of the immunosuppressive drug CsA has allowed us to reveal the presence of a second signalling pathway . ^^^ Here , proliferation can be blocked by antibodies to CD 2 or LFA 3 , presumably by blocking of augmented IL 2 production . ( ABSTRACT TRUNCATED AT 400 WORDS ) . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The Ag independent homotypic adhesion induced by mAb L 25 was not inhibited by mAb to the lymphocyte function associated Ag 1 ( CD11a / CD18 ) , CD 2 , CD 4 , and CD 8 , or to their ligands ICAM 1 , LFA 3 , MHC class 1 , or MHC class 2 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Human T cells spontaneously bind sheep E and this reflects physiologic interactions between specific adhesion molecules , principally T cell CD 2 , and the sheep equivalent of LFA 3 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Adhesion was not affected by Mab against the sheep erythrocyte receptor ( LFA 2 ) , a nonpolymorphic HLA class 1 framework antigen , or against LFA 3 , the alpha chain of OKM 1 , or the alpha chain of p 150 , 95 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
As expected , function associated MoAb to CTL antigens ( T 3 , T 8 , LFA 2 ) and target cell antigens ( HLA , LFA 3 ) blocked only CTL clones and not NK effectors . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The human T cell erythrocyte receptor ( CD 2 antigen ) allows thymocytes and mature T cells to adhere to thymic epithelium and target cells through a cell surface protein , LFA 3 ( refs 1 6 ) . ^^^ A large number of mutations selected for loss of antibody reactivity in the first two regions also weaken the CD 2 LFA 3 interaction . ^^^ CD 2 sequences that participate in LFA 3 binding correspond to immunoglobulin variable region hypervariable sequences when the homologous domains are aligned . . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
T cell pellets were employed during incubation to facilitate interaction between T cell LFA 3 and CD 2 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
These data indicate that mAb to certain cell surface differentiation Ag that in soluble form inhibit T cell function such as LFA 1 , LFA 3 , and CD 2 can under appropriate conditions induce co mitogenic signals on T cells . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The interaction of CD 2 with its LFA 3 ligand expressed by autologous erythrocytes results in enhancement of B cell responses . ^^^ These results demonstrate a functional effect on B cells of the interaction between the CD 2 molecule on T lymphocytes and its natural ligand , LFA 3 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Because human E have been shown to interact with human T cells via CD 2 on T cells and LFA 3 on human E , we have studied the ability of human E and T lymphocyte CD 44 Ag to participate in CD2 / LFA 3 interactions between human E and T cells . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Leucocyte interaction with other cells is facilitated by the adhesion molecules , leucocyte function associated antigen 1 ( LFA 1 ) binding to intercellular adhesion molecule 1 ( ICAM 1 ) and for T cells and natural killer ( NK ) cells the binding of LFA 2 ( CD 2 ) to LFA 3 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Murine CD 2 : structure , expression , and recognition of human LFA 3 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Binding of LFA 3 to the T cell surface receptor CD 2 promotes intercellular adhesion and is costimulatory with anti CD 2 mAbs in ' alternative pathway ' activation of T cells . ^^^ Addition of purified T11TS , the sheep form of LFA 3 that binds to human CD 2 but is not recognized by mAb G 26 , restored the T cell response to PHA P but not to ConA , surface aldehydes , anti CD 3 mAb , or allogeneic cells . ^^^ T cell activation by PHA P , but not by the other polyclonal T cell activators studied thus seems to be mechanistically similar to ' alternative pathway ' activation induced by anti CD 2 mAb in that the costimulatory effect of LFA 3 is independent of its prescence on an accessory cell membrane . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
CD 2 serves to facilitate conjugate formation between the T lineage cell and its cognate partner via intermolecular interaction of CD 2 and LFA 3 on the former and latter cells , respectively . ^^^ Perturbation of the CD 2 extracellular segment by certain combinations of anti CD 2 MAbs or LFA 3 and a single anti CD 2 MAb activate T lineage function . ^^^ This relatively low affinity suggests that multivalent interactions among CD 2 monomers on the T cells and individual LFA 3 structures on the cognate partner are important in enhancing the avidity of the T cell interaction with its target or stimulator cell . ^^^ The affinity of the CD 2 extracellular segment for LFA 3 is not affected by truncations in the CD 2 cytoplasmic domain , implying that ligand binding is not regulated by intracellular mechanisms . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Cytolysis by these cells was restricted to targets expressing the appropriate HLA A 2 specificity and was blocked by mAb recognizing CD 2 , CD 3 , CD 8 , LFA 1 , and LFA 3 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The stimulation of nonspecific killing was seen only with MAb to the T 3 antigen ; MAb to other function associated antigens ( e . g . , LFA 1 , LFA 2 , LFA 3 , T 4 , T 8 , HLA A , B , C , and DR ) had no effect . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Afferent dendritic cells express high levels of the cellular adhesion molecule LFA 3 , and at the same time express a ligand for this molecule , namely CD 2 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
LFA 1 is a member of the integrin family of adhesion receptors and one of its ligands is intercellular adhesion molecule 1 ( ICAM 1 ) ; a ligand for CD 2 is LFA 3 ; and ligands for CD 4 and CD 8 appear to be major histocompatibility complex class 2 and class 1 molecules , respectively . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Human memory T lymphocytes express increased levels of three cell adhesion molecules ( LFA 3 , CD 2 , and LFA 1 ) and three other molecules ( UCHL 1 , CDw 29 , and Pgp 1 ) and have enhanced IFN gamma production . ^^^ Studies of cell surface molecules involved in human T cell interaction reveal that differential expression of each of three adhesion molecules ( LFA 3 , CD 2 , and LFA 1 ) subdivides human peripheral blood T cells into major subpopulations . ^^^ Systematic analysis of the relationship between expression of these and other markers of T cell subsets demonstrates a single major subset of human peripheral blood T lymphocytes distinguished by enhanced expression of LFA 3 , CD 2 , LFA 1 , and three other markers ( CDw 29 [ 4B4 ] , UCHL 1 , and Pgp 1 ) . ^^^ Large differences in relative expression are observed for UCHL 1 ( 29 fold ) and LFA 3 ( greater than 8 fold ) , and smaller differences ( 2 to 4 fold ) are seen for CDw 29 , CD 2 , LFA 1 , and Pgp 1 . ^^^ Neonatal T cells ( CD3+ ) are comprised almost exclusively of the subset expressing low LFA 3 , CD 2 , LFA 1 , CDw 29 , and UCHL 1 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
My co workers and 1 have recently shown that an activating signal is mediated by interaction between the T cell surface antigen CD 2 ( T 11 ) and its ligand on sheep erythrocytes ( SE ) , the T 11 target structure ( T11TS ) , which is the homologue to the human LFA 3 antigen . ^^^ Inhibition studies with MoAb suggest that ( 1 ) concurrent stimulation of CD 3 and CD 2 is essential for interleukin 2 production and proliferation , since SE UCHT 1 treated with the anti T11TS MoAb L180 / 1 are not mitogenic ; ( 2 ) proximity of CD 3 and CD 2 is required during the stimulation , since a mixture of SE exposing either anti CD 3 or T11TS is not mitogenic ; and ( 3 ) that T cell AC interactions involving LFA 1 can be replaced by LFA 3 CD 2 interactions , since the anti LFA 1 MoAb 60 . 3 does not inhibit the SE UCHT 1 response , and only partly the SE + PHA response . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The WW 1 BL cells form conjugates with EBV specific CTL through the LFA 1 / ICAM 1 pathway , but in the absence of a target LFA 3 / effector CD 2 interaction these conjugates do not achieve target cell lysis . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Antibody L180 / 1 , specific for the T 11 ( CD 2 ) target structure ( T11TS ) on SE , homologous to the human CD 2 ligand LFA 3 , abolished the response to SENAGO alone or when combined with PEG or TPA . ^^^ The results suggest that ENAGO induce T cell response through CD 2 LFA 3 T11TS interaction , and via other surface antigens bound by the oxidatively induced aldehyde groups on ENAGO . . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
All of the adhesion to EK was inhibited by LFA 1 and ICAM 1 mAbs , but not by HLA DR , CD 2 , or LFA 3 mAbs . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The MRC OX 45 antigen of rat leukocytes and endothelium is in a subset of the immunoglobulin superfamily with CD 2 , LFA 3 and carcinoembryonic antigens . ^^^ The sequence matches particularly well with the extracellular parts of LFA 3 and CD 2 antigens and the first two domains of carcinoembryonic antigen and non specific , cross reacting antigen . . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Since CD 2 can also function as a cell surface ligand for LFA 3 , an antigen expressed on NK targets , the CD 2 molecule may be considered as a potential NK receptor structure . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The molecules LFA 1 , CD 2 ( T 11 , LFA 2 ) and LFA 3 have been shown to be involved in human CTL conjugation with and lysis of specific target cells . ^^^ Here we describe monoclonal antibody inhibition studies using individual monoclonal antibodies and mixes which demonstrate ( 1 ) that LFA 1 , CD 2 and LFA 3 are involved in antigen independent conjugate formation ; and ( 2 ) suggest that CD 2 and LFA 3 are involved in one pathway and LFA 1 in another . ^^^ We confirmed the existence of distinct pathways by the demonstration that LFA 1 dependent adhesion requires divalent cations and is temperature sensitive whereas CD 2 and LFA 3 dependent adhesion does not require divalent cations and is temperature insensitive . ^^^ Together with previous data , our studies suggest that CD 2 on the effector interacts with LFA 3 as its ligand on targets . . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Thymocyte binding to human thymic epithelial cells is inhibited by monoclonal antibodies to CD 2 and LFA 3 antigens . ^^^ We found anti CD 2 ( LFA 2 , T 11 ) , and anti LFA 3 antibodies all inhibited binding of TE cells to thymocytes . ^^^ By using indirect immunofluorescence assays , we determined that cultured TE cells were 90 % LFA 3 positive and CD 2 negative , whereas thymocytes were 10 % LFA 3 positive and 98 % CD 2 positive . ^^^ In contrast , pretreatment of thymocytes with anti CD 2 but not anti LFA 3 antibodies inhibited TE thymocyte binding . ^^^ Thus TE cell thymocyte binding is blocked by antibodies to the CD 2 ( T 11 ) antigen on thymocytes and by an antibody to the LFA 3 antigen on TE cells . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The lymphocyte function associated LFA 1 , CD 2 , and LFA 3 molecules : cell adhesion receptors of the immune system . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Functionally relevant effector antigens include LFA 1 , LFA 2 , LFA 3 , OKT 4 , OKT 8 , OKT 3 , and Ti , the T cell receptor . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
LFA 1 , LFA 2 , and LFA 3 antigens are involved in CTL target conjugation . ^^^ It appears that LFA 1 , LFA 2 , and LFA 3 are cell surface structures involved in strengthening effector target adhesion that accompanies antigen specific recognition . . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Monoclonal antibodies against lymphocyte function associated antigen ( LFA ) 1 , CD 2 , and LFA 3 ( or their Fab fragments ) each consistently inhibit conjugate formation , but only partially ; mixes of alpha LFA 1 with either CD 2 monoclonal antibodies or alpha LFA 3 cause complete inhibition . ^^^ Preincubation studies ( and restricted expression for CD 2 ) demonstrate that in the CD2 / LFA 3 pathway , CD 2 is critical on the effector and LFA 3 on the target and that in the LFA 1 pathway , LFA 1 is critical on the effector . ^^^ These studies demonstrate the general involvement of two pathways of adhesion in human T cell interactions : one involving T cell LFA 1 and the other involving T cell CD 2 binding to target cell LFA 3 . . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Previous studies have shown that the purified T lymphocyte glycoprotein , cluster differentiation 2 ( CD 2 ) ( also known as T 11 , lymphocyte function associated antigen ( LFA ) 2 , and the erythrocyte ( E ) rosette receptor ) interacts with the LFA 3 molecule on human E . ^^^ Thus , the human T lymphocyte CD 2 molecule is a receptor that cross reacts between LFA 3 on human E and T11TS on sheep E , suggesting that LFA 3 and T11TS are functionally homologous ligands . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Data from our laboratory [ 54 , 69 ] , together with the work of Plunkett et al . [ 61 ] and Shaw et al . [ 70 ] suggest that an endogenous ligand for the CD 2 molecule in humans is the LFA 3 molecule . ^^^ The data reviewed in this paper suggest that within the thymus one endogenous ligand for the alternative pathway of thymocyte activation via the CD 2 molecule is the LFA 3 molecule on TE cells . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
MAb inhibition studies indicated that antigen nonspecific adhesion is largely regulated by the LFA 1 , CD 2 ( LFA 2 / T11 ) , and LFA 3 cell surface molecules . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Functional distinctions between the LFA 1 , LFA 2 , and LFA 3 membrane proteins on human CTL are revealed with trypsin pretreated target cells . ^^^ We asked whether we could distinguish the roles of the human lymphocyte membrane proteins LFA 1 , LFA 2 , and LFA 3 in the function of CTL mediated killing . ^^^ Little is known about the functions of these molecularly distinct proteins beyond the facts that 1 ) binding of a monoclonal antibody ( MAb ) to any one of them is sufficient to inhibit killing , 2 ) that in each case inhibition involves prevention of CTL target cell conjugate formation , and 3 ) that MAb to LFA 1 and LFA 2 inhibit best when bound to the CTL , whereas anti LFA 3 inhibits only when bound to the target cell . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Antibody inhibition studies demonstrated that adhesion between T cells and B7 . 1 transfected melanoma cells was mediated by interactions between LFA 1 : ICAM 1 and CD 2 : LFA 3 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Furthermore , costimulatory signals were provided by UV Mph ; CTLA 4 Ig and LFA 3 Ig fusion proteins and Abs to CD 2 , LFA 3 , LFA 1 , and ICAM 1 inhibited UV Mph induced T cell proliferation . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The functional significance , distribution , and structure of LFA 1 , LFA 2 , and LFA 3 : cell surface antigens associated with CTL target interactions . ^^^ MAb to LFA 1 and LFA 2 inhibit function by binding to effector cell surface molecules , whereas anti LFA 3 MAb appear to block by binding to the target cells . ^^^ Together with previously described molecules , LFA 1 , LFA 2 , and LFA 3 demonstrate the complexity of CTL mediated cytotoxicity at the molecular level . . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Three cell surface molecules , designated LFA 1 , LFA 2 , and LFA 3 were identified by mAbs selected for their ability to block cytolysis by an OKT4+ , HLA DR specific CTL line . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Using mAb reactive with T 3 , T 4 , LFA 1 , LFA 2 , LFA 3 , and HLA DR , we found a qualitatively similar but quantitatively different pattern of inhibition of cytolysis as previously established for lymphoid targets by using the same CTL clone . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Three distinct antigens associated with human T lymphocyte mediated cytolysis : LFA 1 , LFA 2 , and LFA 3 . ^^^ Binding of monoclonal antibodies to four types of molecules , LFA 1 , LFA 2 , LFA 3 , and HLA DR , inhibited killing , suggesting that these molecules participate in the CTL target cell interaction . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
We report herein the cell based mechanism ( s ) of LFA3TIP in inhibition by studying the effects of structurally altered LFA 3 Ig fusion proteins on proliferation of human PBL in vitro and on responses of mice transgenic for human CD 2 . ^^^ We show that LFA3TIP inhibition requires expression of both the LFA 3 and CH 2 domains of the fusion protein that bind CD 2 and Fc gamma RI or Fc gamma RIII , respectively . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
ICAM 2 and LFA 1 were undetectable on cultured LC and attempts to induce this expression by different culture conditions remained unsuccessful . mAb against ICAM 1 , LFA 1 , LFA 3 , and CD 2 , continuously present during culture , inhibited the T cell proliferative response to Candida albicans presented by cultured LC . ^^^ Pretreatment of LC and / or T cells with mAb indicated that anti ICAM 1 and anti LFA 3 inhibited at the LC level , whereas anti LFA 1 and anti CD 2 inhibited at the T cell level . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
We found that the presence of a mixture of mAbs specific for CD 2 , LFA 3 , LFA 1 alpha and beta chain , and ICAM 1 during primary MLC leads to down regulation of secondary proliferative responses to Ag from the donor used for priming . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The expression of cell adhesion molecules ( LFA 1 , LFA 2 , LFA 3 and ICAM 1 ) in skin lesions of leprosy patients was studied , as well as the in vitro expression of these molecules induced with gamma interferon ( IFN gamma ) . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The genes for huICAM 1 ( a ligand for LFA 1 ) and huLFA 3 ( a ligand for CD 2 ) were transfected singly and in combination into a null mouse melanoma background , and clonal populations of cells that stably express ICAM 1 and / or LFA 3 were derived . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Combinations of mAbs directed against T cell adhesion molecules ( LFA 1 , CD 2 , VLA 4 ) or EC adhesion molecules ( ICAM 1 , and 2 , LFA 3 , VCAM 1 ) were able to block T cell proliferation for 100 and 80 % respectively . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Experiments with blocking Abs implicate T cell CD 2 and its endothelial cell ligands , both LFA 3 and CD 59 , but not T cell CD 28 and its putative ligands , in EC induced resistance to CsA . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
After stimulation of gingival cells in vitro , the per cent CD 2 positive T cells and LFA 3 and ICAM 1 positive B cells remained relatively stable over the six day culture period , although P . gingivalis and F . nucleatum appeared to induce an increase in the percentage of gingival T cells expressing LFA 1 . ^^^ In contrast to the gingival lymphocytes , stimulation of peripheral blood cells resulted in an increase in the per cent CD 2 positive T cells , LFA 3 and ICAM 1 positive B cells , with a decrease in LFA 1 positive T cells . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The ability of molecules such as CD 2 and CD 28 , interacting with their ligands LFA 3 and B 7 , to provide signals that protect T cells from the induction of anergy , has been reported . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Increased adherence of CD 2 peripheral blood lymphocytes to cytomegalovirus infected fibroblasts is blocked by anti LFA 3 antibody . ^^^ All subpopulations of CD2+ lymphocytes , namely CD3+ , CD4+ , and CD8+ cells , demonstrated increased adhesion to CMV infected fibroblasts , suggesting that the CD 2 LFA 3 interaction was an important component of the increased binding . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Transfection of ICAM 1 or LFA 3 in CD32+ L cells resulted in enhanced proliferative responses of CD4+ T cell clones after activation by anti CD 3 mAb , whereas transfection of B 7 in CD32+ L cells enhanced proliferative responses of CD4+ T cell clones after activation by anti CD 2 mAb . ^^^ IL 10 inhibited the proliferation of T cell clones induced by anti CD 2 or anti CD 3 mAb on CD32+ L cells expressing these accessory molecules , indicating that interactions of LFA 3 , ICAM 1 , and B 7 with their ligands on T cells did not overcome the inhibitory effects of IL 10 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
To examine whether sgp 60 , which has been proposed as the murine homologue of human LFA 3 , is another ligand for mouse CD 2 , we performed the characterization of sgp 60 by using mouse CD 2 human IgG chimeric protein ( mCD2Rg ) and anti mouse monoclonal antibodies ( mAb ) . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
In contrast , CD 28 ligand B 7 and CD 2 ligand leukocyte function associated Ag ( LFA 3 ) can support IL 2 synthesis and proliferation of Ag specific T cells more efficiently than that of resting T cells . ^^^ Similar coligation of the TCR with CD 2 or CD 28 using either mAb or ligands LFA 3 or B 7 not only lacked the ability to induce death but also failed to reverse or inhibit integrin facilitated death of DRw 6 specific T cells . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
METHODS Using an indirect immunoperoxidase technique we assessed the expression of the cytokines interleukin 1 alpha ( IL 1 alpha ) , interleukin 1 beta ( IL 1 beta ) , interleukin 8 ( IL 8 ) , transforming growth factor beta ( TGF beta ) and granulocyte macrophage colony stimulating factor ( GM CSF ) , of the adhesion molecules intercellular adhesion molecule 1 ( ICAM 1 ) , lymphocyte function associated antigen 1 ( LFA 1 ) , the activated molecular form of LFA 1 ( NKI L 16 ) , CD 2 , and LFA 3 , and of a panel of cellular markers in the minor salivary glands . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
This liver associated lymphocyte population also presented a significantly higher percentage of Mac 1 , ICAM 1 , and LFA 3 and an increased surface expression of LFA 1 , LFA 2 , and NCAM in comparison with peripheral blood lymphocytes . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The analyzed molecules included the beta 1 integrin or VLA family VLA 1 6 , the beta 2 integrins or leukocyte integrins LFA 1 , Mac 1 , and p 150 , 95 , the immunoglobulins LFA 3 , CD 2 , ICAM 1 and VCAM 1 and the selectins ELAM 1 and GMP 140 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Adhesion was also unaffected by antibodies to LFA 1 , ICAM 1 , ICAM 2 , LFA 2 , or LFA 3 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Moreover , since murine fibroblasts were found not to express CD 48 , our results implicate a novel ligand for CD 2 , possibly a homologue of the human LFA 3 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The proliferation of CD 8 T cells is inhibited by mAbs reactive with CD 8 or HLA A and B molecules but not with CD 4 or HLA DR . mAb blocking studies show a role for CD 2 , LFA 3 , and CD 59 , but not for intercellular adhesion molecule 1 , intercellular adhesion molecule 2 , very late activation Ag 4 , vascular cell adhesion molecule 1 , CD 28 , or CD 28 ligand , as costimulatory molecules . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Two ligand receptor pairs may play a role in the adhesion of cytotoxic T cells to their targets : 1 ) intercellular adhesion molecule 1 ( ICAM 1 ) and lymphocyte function associated antigen 1 ( LFA 1 ) , and 2 ) LFA 3 and CD 2 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The high expression of CD 2 on repopulating thymoblasts may be an additional indication of their activated state and for a role of interaction with the ligand LFA 3 on thymic epithelial cells during this phase of thymocyte differentiation . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
As in the previously reported cold dependent reversible rosetting , stable rosetting is mediated by the erythrocyte LFA 3 and lymphocyte CD 2 molecules . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Concurrently , we observed a reduced expression of IL 2 alpha chain receptors on surfactant treated CD56+ / CD3 cells and a dramatic reduction in the expression of adhesion molecules including CD 2 , LFA 1 , LFA 3 , and ICAM 1 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
In fact , a receptor ligand interaction occurs between CD11a / CD18 ( LFA 1 ) and CD 2 molecules , expressed on the effector cells , and the respective counterparts on target cells , i . e . , ICAM 1 , ICAM 2 , or LFA 3 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
CSF T cells also expressed significantly higher levels of several adhesion and activation molecules , including very late activation ( VLA ) antigens 3 through 6 , lymphocyte function associated ( LFA ) antigen 1 , LFA 3 , CD 2 , CD 26 , and CD 44 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The molecules we analyzed included the VLA family VLA 1 6 , the leukocyte integrins LFA 1 , Mac 1 and p 150 , 95 , the immunoglobulins LFA 3 , CD 2 , ICAM 1 and VCAM 1 and the selectin ELAM 1 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
AML cells from the majority of patients with leukaemia at diagnosis or relapse expressed the ligand pair LFA 1 and ICAM 1 , the CD 2 ligand LFA 3 , alpha and beta chains of the integrins VLA 4 , VLA 5 and VLA 6 , and the hyaluronate receptor CD 44 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
This adherence was blocked completely by antibody to IL 2 receptor ( IL2R ) beta and , partially , by antibodies to beta 1 or beta 2 integrins , ICAM 1 , CD 2 or LFA 3 , but not by antibodies to the IL2R alpha or CD 56 antigen . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The protein sequence data support the view that Humly 9 belongs to the immunoglobulin superfamily subgroup which includes CD 48 , CD 2 , and LFA 3 . . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The inhibition of IL 1 beta mRNA induction by anti CD 2 mAb was restricted to only those mAb that block CD2 / CD58 ( LFA 3 ) interactions . ^^^ These results indicate that CD 2 is required for T cell induction of IL 1 beta mRNA through interaction with LFA 3 on the monocyte and that the generation of soluble IL 1 beta is regulated by CD69 . . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Expression of LFA 3 , CD 2 , CD 3 and CD 14 was visualized by an immunoperoxidase technique and assessed microscopically . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
In contrast , two chimeric receptors , In which 21 of the amino acids in the distal transmembrane region of Fc gamma RI were replaced with the transmembrane region of the related receptors CD 2 or LFA 3 , were expressed but failed to interact functionally with gamma chain to mediate phagocytosis . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
LFA 1 and ICAM 1 ICAM 3 , CD 2 and LFA 3 ; receptors for TNF and corresponding ligand etc . 0 ; ( 2 ) the presence of multiple disorders in the organization of `` extracellular matrix surface membrane submembrane cytoskeleton ' ' assembly of the NK sensitive TCs ; ( 3 ) non specific primary linking of NK cell with TCs , which induces a transfer of vesicles or membrane fragments from the NK surface to the target cell surface ( and perhaps vice versa ) . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
To optimize engraftment , and thereby survival , further , the additional blockade of a second important leukocyte adhesion and signalization pathway mediated by the CD 2 and LFA 3 interaction was attempted in a multicenter protocol conducted by the European Bone Marrow Transplantation European Society for Immunodeficiency group . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
CD 2 , LFA 1 , and CD 28 are the major costimulatory and adhesive molecules on T cells and bind to the LFA 3 , ICAM 1 and B 7 ligands , respectively , on APC . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Significant task by gender interactions indicated that during stress women increased whereas men decreased in 3 markers of the integrin family , LFA 1 , LFA 2 , and LFA 3 ( all p < 0 . 05 ) . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Since the murine CD 48 is functionally homologous to the human CD 2 ligand LFA 3 ( CD 48 ) , the function of the human CD 48 remains unknown . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Flow cytometry analysis of the phenotypic distribution of MNC revealed age related differences in the expression of CD 3 , CD 4 , CD 8 , CD 14 , CD 19 , CD45RA , CD45R0 , CD 2 , LFA 1 , ICAM 1 and LFA 3 . ^^^ The TNF alpha levels in suboptimally stimulated cultures correlated negatively with the expression of LFA 3 and positively with CD45RA , while IFNg correlated positively with CD 2 , LFA 1 , CD45R0 and CD 8 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
There was no change in other lymphocyte adhesion molecules expression ( ICAM 1 , LFA 3 or CD 2 ) at day 4 , although LFA 3 and CD 2 expression was moderately decreased at day 28 and day 90 respectively ; nor was there any change in the distribution of lymphocyte subsets ( CD 4 , CD 8 , and CD45RA , CD45RO ) , although a small decrease in CD45RO circulatory T cells was noted at day 28 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
We also provide evidence that GCS inhibit cell aggregate formation induced by TCR ligation , which directly correlates with the down modulation of LFA 1 and CD 2 , but not LFA 3 or ICAM 1 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
B 7 expressing melanoma cells were excellent inducers of T cell proliferation , cytokine production , and cytolytic activity in allogeneic mixed lymphocyte cultures through a process dependent on the function of the T cell receptor as well as interactions between B 7 : CD 28 , CD 2 : LFA 3 , and LFA 1 : ICAM 1 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The expression of ICAM 1 , ICAM 3 , LFA 3 , CD 2 , LFA 1 , VLA 4 , CD1a , VCAM 1 , CD 69 , and activated b 1 integrins were assessed by immunostaining . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Major histocompatibility complex class 2 , CD 44 , CD 2 , and LFA 3 expression was unchanged during pregnancy , whereas CD 25 , VLA 4 and L selectin were never expressed . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Analysis of the CD 44 modified LFA 3 protein showed that it retains the ability to engage and trigger the function of its natural ligand CD 2 , resulting in T cell activation . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The major ligand of CD 2 is CD 48 , the murine analog of human leukocyte function associated antigen 3 ( LFA 3 ) . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Ligation of 2B4 with monoclonal antibodies increases target cell lysis and IFN gamma production . 2B4 is the high affinity counter receptor for CD 48 in mice and humans . 2B4 L is a member of the CD 2 subgroup of the immunoglobulin supergene family , which includes CD 48 , LFA 3 , CD 84 , Ly 9 and SLAM . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
This was accounted for in part by minor heterogeneity in the LFA 3 / IgG1 preparations , and potentially by the ability of the ligand to bind to both CD 2 and Fc receptors ( FcR ) , best evidenced by the distinct binding properties of the fusion protein to NK and T cells . ^^^ The low affinity LFA 3 / IgG 1 binding to T cells is consistent with binding to CD 2 only , and is in agreement with the low affinity reported for interactions between soluble forms of LFA 3 and CD 2 by surface plasmon resonance technology . ^^^ Moreover , as the low affinity determinations are similar for CD 2 on resting and activated T cells , although the CD 2 molecule has been reported to be altered to reveal new epitopes upon T cell activation , the binding data argue against multiple cell activation dependent affinity states of CD 2 for LFA 3 binding . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
The T cells expressed lymphocyte function associated antigen ( LFA ) 1 and CD 2 , and the synovial cells expressed intracellular adhesion molecule ( ICAM ) 1 and LFA 3 , suggesting possible interactions via LFA 1 / ICAM 1 and CD2 / LFA 3 . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Adhesion molecules lymphocyte function associated antigen ( LFA ) 1 and CD 2 on T cells recognize intercellular adhesion molecule ( ICAM ) 1 and LFA 3 on APCs , respectively . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
In contrast , antibodies against CD 2 and LFA 3 had no effect on the lytic activity of 5 gamma 9 / V delta 2 T cells , but strongly inhibited the cytokine release and proliferation . ^^^ However , the CD 2 LFA 3 interaction was not an absolute requirement for the cytokine release and the DNA synthetic activity of antigen stimulated 5 gamma 9 / V delta 2 T cells , since the inhibitory effect could be reversed by addition of exogenous interleukin 2 ( IL 2 ) . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Possible unique adhesions of DIL : UtMVEC Myo included SLe ( 10 ) : PECAM , vascular cell adhesion molecule 1 : alpha 4 , and LFA 2 : LFA 3 , whereas DIL : STO included LFA 2 : LFA 3 and N CAM : N CAM . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Alefacept , an immunomodulatory recombinant LFA 3 / IgG1 fusion protein , induces CD 16 signaling and CD2 / CD16 dependent apoptosis of CD 2 ( + ) cells . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
Furthermore , higher levels of LFA 3 engagement of CD 2 rendered CD8+ T cells unresponsive to secondary antigenic re challenge . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
To gain a profound insight into the underlying mechanisms , we phenotypically characterized pan leukocytes ( CD 18 ) , naive T lymphocytes ( CD 3 , CD 2 , CD 4 , CD 8 ) , activated lymphocytes ( LFA 1 , LFA 3 , VLA 4 , ICAM 1 , CD 69 , CD45R0 ) , macrophages ( Mac 1 , 27E10 ) , B lymphocytes ( CD 19 ) and NK cells ( CD 57 ) in DCM and control hearts . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
It is an immunomodulatory recombinant , fully human lymphocyte function associated antigen 3 / immunoglobulin G 1 fusion protein ( LFA 3 Ig ) CD 2 antagonist that targets memory effector T cells by binding CD 2 on the T cell surface . ^^^
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA
Interacting proteins: P19256 and P06729	Pubmed	SVM Score :0.0
NA