| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.86533132 |
| We have identified CD 59 , a glycoprotein with complement inhibitory function , as a second physiological ligand for CD 2 . 0.86533132^^^ These observations suggest that direct interactions between CD 2 and both CD 58 and CD 59 contribute to T cell activation and adhesion . . 0.75807107^^^ |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.56453494 |
| These results directly show that CD 2 binds CD 59 and that subtle molecular changes occur upon binding . . 0.56453494^^^ |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.62529193 |
| Monoclonal antibodies ( mAb ) against the adhesion molecules CD 2 and CD 28 are likely to mimic the interaction with their respective physiological ligands [ lymphocyte function associated antigen 3 ( LFA 3 ) / CD58 , CD 59 and CD 48 for CD 2 , B7 / BB1 for CD 28 ] . 0.62529193^^^ |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| The glycosylphosphatidylinositol ( GPI ) anchored CD 59 protein ( human protectin ) protects cells against complement induced lysis , binds to CD 2 and also transduces activation signals within T cells . ^^^ |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| Human cell adhesion molecule CD 2 binds CD 58 ( LFA 3 ) with a very low affinity and an extremely fast dissociation rate but does not bind CD 48 or CD 59 . ^^^ Reported ligands for human CD 2 include the structurally related IgSF CAMs CD 58 ( LFA 3 ) and CD 48 as well as , more controversially , the unrelated cell surface glycoprotein CD 59 . ^^^ In the present study we have used surface plasmon resonance to obtain definitive affinity and kinetic data on the interactions of a soluble , recombinant form of human CD 2 with soluble forms of CD 58 , CD 48 , and CD 59 . ^^^ Binding of CD 2 to CD 58 was readily detected but we were unable to detect any direct interaction between CD 2 and either CD 59 or CD 48 under conditions in which very low affinity interactions ( Kd approximately 0 . 5 mM ) would have been detected . ^^^ |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| CD 58 is the principal ligand for human CD 2 ; CD 59 and CD 48 are two additional , low affinity ligands that have been defined for CD 2 . ^^^ We have shown that CD 59 enhanced CD 58 dependent T cell proliferation and IL 2 secretion in the presence of suboptimal concentration of PHA or a submitogenic combination of stimulatory anti CD 2 mAbs , presence of suboptimal concentration of PHA or a submitogenic combination of stimulatory anti CD 2 mAbs , T 11 2 + T 11 3 . ^^^ As expected , CD 59 costimulation of CD 58 dependent T cell proliferation was inhibited by Abs directed against CD 59 , CD 58 , and CD 2 . ^^^ |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| Experiments with blocking Abs implicate T cell CD 2 and its endothelial cell ligands , both LFA 3 and CD 59 , but not T cell CD 28 and its putative ligands , in EC induced resistance to CsA . ^^^ |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| In the absence of gp 120 , CD 4 displayed high association with CD 3 , CD 5 , CD45RC , CD 25 , CD 28 , CD 44 , and CD 53 ; weak association with CD 2 , CD 38 , CD45RB , CD62L , and CD 26 ; and no association with CD45RA , CD45RO , CD11b , CD11a , CD 54 , CD 7 , CD 48 , CD 98 , CD 59 CD55 , HLA class 1 and class 2 molecules . ^^^ |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| Through physiologic interactions with its ligands CD 58 ( lymphocyte function associated Ag 3 , LFA 3 ) and CD 59 , the T cell glycoprotein CD 2 plays a role in T cell signaling and promotes lymphocyte adhesion . ^^^ |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| However , binding studies on CD 48 , CD 58 , or CD 59 transfected CHO cells with spCD 2 under low stringency wash conditions revealed that human CD 48 is a low affinity ligand of human CD 2 compared with CD 58 ( Kd approximately 10 ( 4 ) vs . approximately 10 ( 6 ) M , respectively ) . ^^^ No detectable binding was observed to CD 59 transfected CHO cells despite a report suggesting that CD 59 may bind to the human CD 2 adhesion domain . ^^^ |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| The CD 2 molecule plays an important role in T cell adhesion by interacting with the ligands CD 58 ( LFA 3 ) and CD 59 . ^^^ Subsequent studies demonstrated that the putative new ligand is not CD 59 , and that it interacts with a different region of the CD 2 molecule than CD 58 , probably a site located between the T 11 ( 1 ) and T 11 ( 2 ) epitopes . ^^^ |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| Expression of CD 59 , a complement regulator protein and a second ligand of the CD 2 molecule , and CD 46 in normal and neoplastic colorectal epithelium . ^^^ CD 59 , a phosphatidyl inositol anchored glycoprotein , inhibits the formation of the terminal membrane attack complex ( MAC ) of complement and was found to be a second ligand for CD 2 contributing to T cell activation . ^^^ |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| However , recent studies have shown that in addition to its role as a complement inhibitor , CD 59 binds human T cell CD 2 and contributes to T cell activation . ^^^ |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| The proliferation of CD 8 T cells is inhibited by mAbs reactive with CD 8 or HLA A and B molecules but not with CD 4 or HLA DR . mAb blocking studies show a role for CD 2 , LFA 3 , and CD 59 , but not for intercellular adhesion molecule 1 , intercellular adhesion molecule 2 , very late activation Ag 4 , vascular cell adhesion molecule 1 , CD 28 , or CD 28 ligand , as costimulatory molecules . ^^^ |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| The T cell and NK cell adhesion molecule CD 2 interacts with different ligands , viz , CD 58 , CD 48 , and CD 59 . ^^^ |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| CD 59 and CD 48 expressed by rat retinal pigment epithelial cells are major ligands for the CD 2 mediated alternative pathway of T cell activation . ^^^ Rat RPE cells expressed high levels of CD 59 and low levels of another potential CD 2 ligand , CD 48 , both in vitro and in the in vivo model of experimental autoimmune uveoretinitis . ^^^ Ab cross linking of CD 48 but not CD 59 on the RPE was found to induce messenger RNA expression for IL 1 beta , which together with constitutively expressed IL 6 are required costimulatory factors for T cell activation through CD 2 . ^^^ |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| In the two patients that were tested , the GPI anchored surface molecules CD 55 and CD 59 were also absent on the CD 52 cells , although expression of other cell surface transmembrane , proteins ( CD 3 , CD 4 and CD 2 ) was unaffected . ^^^ |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| Two types of large complexes containing protein tyrosine kinases were observed : the first one contained CD 3 and the transmembrane proteins CD 2 , CD 4 , CD 5 , CD 6 , CD 7 , CD 8 , CD11a , CD 38 , CD 43 , CD 45 , CD 71 , CD 98 and CD 99 and the other contained mainly the GPI anchored proteins CD 48 , CD 55 , CD 59 and CDw 108 as well as a fraction of CD 4 and CD 8 . ^^^ |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| This was due to the complete loss of Fas and TNFR surface expression and , in the case of Jurkat derived sub lines , also of CD 3 , CD 2 and CD 59 molecules . ^^^ |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| All glycosylphospha tidylinositol ( GPI ) anchored proteins ( CD 14 , CD16B , CD 48 , CD 59 , CD 87 and GPI anchored versions of CD 2 and CD 25 ) and protein tyrosine kinase ( PTK ) Lck but not transmembrane proteins ( CD 2 , CD 4 , CD 5 , CD 6 , CD 8 ) and PTK ZAP 70 were in part localized in buoyant , lipid rich , detergent resistant membrane GPI microdomains of the COS cells . ^^^ |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| Unstimulated human PBMC were cultured for 9 days with mAb directed at CD2 / CD48 , / CD58 , CD 59 , CD5 / CD72 , CD11a CD18 / CD54 , CD28 / CD80 , CD 86 , CD40 / CD40L , or rat CD 2 ( control ) . ^^^ |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| Recombinant soluble forms of six leukocyte antigens belonging to the Ly 6 ( CD 59 ) , scavenger receptor ( CD 5 ) , and immunoglobulin ( CD 2 , CD 48 , CD 4 , and Thy 1 ) superfamilies were expressed in the same Chinese hamster ovary cell line , thus providing an opportunity to examine the extent to which N linked oligosaccharide processing might vary in a superfamily , domain , or protein dependent manner in a given cell . ^^^ |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Among other features , pathologic MCs show aberrant expression of CD 25 and CD 2 antigens and abnormally high levels of the CD11c and CD 35 complement receptors , the CD 59 complement regulatory molecule , the CD 63 lysosomal membrane antigen , and the CD 69 early activation antigen . ^^^ |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| CD 2 mediated CD 59 stimulation in keratinocytes results in secretion of IL 1alpha , IL 6 , and GM CSF : implications for the interaction of keratinocytes with intraepidermal T lymphocytes . ^^^ Binding of keratinocytes to HL 60 cells that express CD 59 ligand CD 2 induced the same pattern of secreted cytokines whereas binding to CD 2 negative HL 60 cells did not . ^^^ The results demonstrate that CD 2 mediated CD 59 stimulation in human keratinocytes leads to synthesis of a particular set of cytokines implying a potential activation pathway in the interaction of keratinocytes with intraepithelial CD2+ T cells . . ^^^ |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| Anti CD 59 monoclonal antibodies ( MoAb ) blocked the cytotoxicity to the same degree as anti CD 3 , HLA DR or CD 2 mAb . ^^^ |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13987 and P06729 |
Pubmed |
SVM Score :0.0 |
| NA |
|