| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.90068774 |
| The finding that CD 2 is associated with p56lck and p59fyn in detergent lysates suggests that interactions with these Src like protein kinases play a critical role in CD 2 mediated signal transduction . . 0.90068774^^^ |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :1.1482875 |
| Under these conditions CD 2 was found to be associated with the protein tyrosine kinases p56lck and p59fyn as well as two low molecular weight phosphoproteins . 1.1482875^^^ |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.84641177 |
| Using in vitro kinase assays with immune complexes precipitated from cell lysates , we demonstrate that CD 2 can associate with p56lck and p59fyn in a rat thymoma line that does not express CD 4 or CD 8 , and in a TcR negative Jurkat cell line . 0.84641177^^^ In TcR positive Jurkat cells that express rat CD 2 , interaction of CD 2 with p56lck and p59fyn was clearly seen , but it was absent in cells where the cytoplasmic tail of CD 2 is truncated , indicating that the interactions are mediated by the cytoplasmic region of CD 2 . 0.76064051^^^ Furthermore , using cells expressing CD 2 molecules with partial truncations in the cytoplasmic domain , we show that the association of CD 2 with p56lck is progressively lost as the cytoplasmic domain is shortened , and that the capacity of the mutants to associate with p56lck correlates with their capacity to transduce transmembrane signals . . 0.60478839^^^ |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.77539508 |
| CD 2 has been shown previously to associate physically with the Src family protein tyrosine kinases p 56 ( lck ) and p 59 ( fyn ) . 0.77539508^^^ |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| The lymphocyte specific tyrosine protein kinase p56lck is endocytosed in Jurkat cells stimulated via CD 2 . ^^^ Incubation of the human T cells , Jurkat , with two sets of activating anti CD 2 mAb ( T 11 ( 2 ) + T 11 ( 3 ) , D 66 + T 11 ( 1 ) ) induced delocalization of p56lck and CD 2 receptors from the plasma membrane and increased the tyrosine kinase activity of p56lck . ^^^ The anti CD 2 mAb combination ( T 11 ( 2 ) + T 11 ( 3 ) ) that produced the most rapid increase in p56lck kinase activity also induced the most rapid delocalization of the kinase . ^^^ In stimulated cells , both p56lck and CD 2 receptors are detected in cytoplasmic vesicles . ^^^ By double staining it was shown that only part of the p56lck colocalized with the internalized CD 2 receptor suggesting distinct sorting processes . ^^^ |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| Importantly , T lymphocyte activation employing CD 2 monoclonal antibodies ( mAb ) , CD 3 mAb or phorbol 12 , 13 dibutyrate results in loss of pp 29 and pp 32 from the CD45 / p56lck molecular complex and concomitant association of two distinct phosphoproteins with different molecular weights ( pp 30 and pp 31 ) . ^^^ |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| Both T cell receptor ( TcR ) CD 3 complex and CD 2 increase the tyrosine kinase activity of p56lck . ^^^ CD 2 can mediate TcR CD 3 independent and CD 45 dependent activation of p56lck . ^^^ This extends our previous findings which demonstrated the involvement of p56lck kinase activity in the CD 2 signal transduction pathway . ^^^ Therefore , to test directly the role of TcR CD 3 in CD 2 induced activation of p56lck , we utilized mutant variants of the Jurkat cell line lacking in cell surface TcR CD 3 . ^^^ We found that cell surface expression of TcR CD 3 is not required for the activation of p56lck via CD 2 but is necessary for the appearance of the reduced electrophoretic mobility form of p56lck observed after CD 2 triggering . ^^^ |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| The tyrosine kinase activity of p56lck is increased in human T cells activated via CD 2 . ^^^ We have previously shown that p56lck , a lymphocyte specific protein tyrosine kinase , is hyperphosphorylated on serine and tyrosine residues 15 minutes after activation via CD 2 with a concomitant shift to a higher molecular mass . ^^^ We now demonstrate that the tyrosine kinase activity of p56lck is increased within seconds following CD 2 triggering . ^^^ These results suggest that p56lck may play an important role in the CD 2 activation pathway . . ^^^ |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| The lymphocyte specific protein tyrosine kinase p56lck is hyperphosphorylated on serine and tyrosine residues within minutes after activation via T cell receptor or CD 2 . ^^^ Within minutes after activation of a human T cell derived line ( Jurkat ) via stimulation of either the TcR CD 3 complex or the CD 2 glycoprotein , we observed a hyperphorphosylation of p56lck . ^^^ |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| In this study we examine the ability of tyrosine phosphorylated proteins from Jurkat T cells stimulated by CD 2 or T cell receptor ( TcR ) CD 3 to interact with the src homology 2 ( SH 2 ) domains from p56lck ( Lck ) . ^^^ |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| Importantly , this CD 2 induced tyrosine phosphorylation of p 63 can also occur in the absence of the CD 3 zeta chain membrane expression , and is also distinct from the protein tyrosine kinases p56lck and p59fyn . ^^^ |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| Defective cytokine expression but adult type T cell receptor , CD 8 , and p56lck modulation in CD 3 or CD 2 activated T cells from neonates . ^^^ The results indicate that in newborn cells , despite a reduced lymphokine expression observed after CD 2 or CD 3 MAb activation , the up regulation of the T cell receptor , CD 8 , and p56lck was similar to that found in adult cells , as was also found after phytohemagglutinin activation of both types of cells . ^^^ |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| In human T cells CD 45 is reported to associate with both cell surface and intracellular molecules including CD 2 , CD4 / CD8 , CD 5 , p56lck and p59fyn . ^^^ |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| In contrast , activation of the CD 2 pathway stimulated the kinase activity of p56lck , but did not induce a significant shift in NK cells , as opposed to T lymphocytes . ^^^ |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| Similarly , activation and internalization of p56lck were observed using a combination of anti CD 45 ( MC5 / 2 ) and anti CD 2 ( T 11 ( 2 ) ) mAb , suggesting that a functional complex consisting of CD 45 , CD 2 and p56lck was formed upon cell triggering . ^^^ Taken together , these results suggests that : ( 1 ) CD 45 participates in the regulation of p56lck kinase activity in vivo and that ( 2 ) CD 45 could play a mediator role in the stimulation and endocytosis of p56lck through the CD 2 pathway . . ^^^ |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| Using in vitro kinase assays on CD 2 immunoprecipitates from detergent lysates of T lymphocytes , we identified a complex consisting of CD 2 , zeta chain and the src family kinases p59fyn and p56lck . ^^^ |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| New insights have been gained in several areas : the avidity regulation of beta 1 and beta 2 integrins and their role in signal transduction ; the regulation of CD 8 avidity ; the role of Lck in CD 4 coreceptor activity ; and the novel role for CD 2 adhesion in the T cell antigen response . . ^^^ |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| P56lck A lymphocyte specific protein tyrosine kinase : activation , regulation and signal transduction . p56lck , a src family protein tyrosine kinase interacts with several T cell receptors , like : CD 4 , CD 8 , CD 2 and the beta chain of the IL 2 , thereby receptors devoid of kinase activity may transduce signals via tyr phosphorylation . ^^^ Upon activation through the CD 2 or the CD 45 receptors the kinase activity of p56lck is temporarily increased . ^^^ By immunofluorescent and confocal microscopy we observed that a significant proportion of p56lck and CD 2 receptors are localized in endosomal vesicles after stimulation . ^^^ |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| Anti CD 2 induced tyrosine phosphorylation of T cell polypeptides is independent of the PMA induced modification of p56lck . ^^^ We find that with human peripheral T cells the serine phosphorylation of p56lck is independent of the more rapid tyrosine phosphorylation of other polypeptides via stimulation of the CD 2 receptor with anti CD 2 ( anti T 11 ( 2 ) and anti T 11 ( 3 ) mAb ' s ) . ^^^ |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| Crosslinking CD 28 causes a shift in the relative mobility of p56lck from 56 to 60 kD similar to that seen after crosslinking of CD 2 and CD 4 , cell surface receptors known to be associate with and activate p56lck . ^^^ |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| The SH 3 domain of p56lck binds to proline rich sequences in the cytoplasmic domain of CD 2 . ^^^ An Src like protein tyrosine kinase , p56lck , coprecipitates with CD 2 , and perturbation of CD 2 by monoclonal antibodies results in an increase in the activity of p56lck , suggesting that an interaction with p56lck contributes to CD 2 mediated signaling . ^^^ Herein , we investigate the mechanism by which CD 2 associates with p56lck . ^^^ We demonstrate that CD 2 and p56lck associate when coexpressed in nonlymphoid cells , that this association requires the cytoplasmic domain of CD 2 , and that the SH 3 domain of p56lck mediates its interactions with CD 2 . ^^^ Using truncation mutants of CD 2 , we identify two regions in the cytoplasmic domain of CD 2 involved in binding p56lck . ^^^ |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| We have previously established that upon CD 2 activation of T cells , p 56 ( lck ) showed a transient increase in its kinase activity and was partially internalized . ^^^ Here we studied the possibility that p 56 ( lck ) could retain its kinase activity in the endosomes of CD 2 triggered cells . ^^^ In CD 2 triggered cells , part of Lck was internalized and presented a maximal kinase activity in the endosome enriched fraction after 5 min , decreasing thereafter . ^^^ We demonstrated that the protein tyrosine kinase ZAP 70 was recruited to the endosomal fraction upon CD 2 stimulation with kinetics similar to that of p 56 ( lck ) , suggesting that recruitment of protein tyrosine kinases to endosomal vesicles could promote specific transduction signals at the intracellular level . . ^^^ Recruitment of activated p56lck on endosomes of CD 2 triggered T cells , colocalization with ZAP 70 . ^^^ |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| All T cell lines tested were positive for TCF 1 and GATA 3 , which are the transcription factors for promoters for several T cell restricted markers , including CD 2 , CD 3 , TcR , and lck . ^^^ |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| In Jurkat cells , p56lck is required for CD 3 or CD 2 induced tyrosine phosphorylations and clearly activated after CD 2 cross linking . ^^^ Altogether , these findings suggest that CD 2 could bypass the PTK ZAP 70 for PLCgamma 1 activation and involves a preferential cascade comprising p56lck and a p 62 protein , possibly acting as an anchor molecule . . ^^^ |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| Ligation of the CD 2 cell surface glycoprotein expressed on T lymphocytes and NK cells induces protein tyrosine phosphorylation and activation of the Src kinases , LCK and FYN . ^^^ With the use of different Jurkat cell mutants it was demonstrated that CD 2 mediated activation of EMT required expression of LCK , but not require surface expression of the CD 3 zeta chain . ^^^ |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| These effects do not correlate with changes in the expression of cell surface molecules implicated in antigen recognition , like TCR / CD3 , CD 2 , LFA 1 , or CD 45 , or with lower p56lck or p59fyn activity in the mutant cells . ^^^ |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| However , using CD 2 stimulated cells , we observe that p 56 ( lck ) activation by itself does not induce Sam 68 tyrosine phosphorylation . ^^^ |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| CD 2 has a relatively long cytoplasmic tail which associates with the src family tyrosine kinases , p 56 ( lck ) and p 59 ( fyn ) , and could potentially signal directly . ^^^ |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| All glycosylphospha tidylinositol ( GPI ) anchored proteins ( CD 14 , CD16B , CD 48 , CD 59 , CD 87 and GPI anchored versions of CD 2 and CD 25 ) and protein tyrosine kinase ( PTK ) Lck but not transmembrane proteins ( CD 2 , CD 4 , CD 5 , CD 6 , CD 8 ) and PTK ZAP 70 were in part localized in buoyant , lipid rich , detergent resistant membrane GPI microdomains of the COS cells . ^^^ |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| Expression of active p56lck also generates double positive ( DP ) thymocytes with low levels of CD 2 antigen expression . ^^^ Again , p56lck can not prevent expression of CD 2 when expressed on a Rho background . ^^^ The signaling pathways that are mediated by p56lck and control thymocyte proliferation , alpha / beta TCR and CD 2 antigen expression can thus be distinguished by their dependency on Rho function . . ^^^ |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| Here , we describe transgenic mice that express C 3 transferase under the control of the locus control region ( LCR ) of the CD 2 gene ; this regulatory element drives expression at a later stage of thymocyte development than the lck proximal promoter [ 7 ] . ^^^ In these mice , thymocyte numbers were also reduced by 50 to 100 fold , but unlike the lck C 3 mice , in which the reduction predominantly results from defects in cell survival of CD 25 ( + ) thymocyte progenitors , the CD 2 C3 transgenic mice had a pre T cell differentiation block at the CD 25 ( + ) stage after rearrangement of the T cell receptor ( TCR ) beta chains . ^^^ |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| Here we demonstrate that Lck and Fyn can be activated by proline motifs in the CD 28 and CD 2 proteins , respectively . ^^^ |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| MHC class 1 cross talk with CD 2 and CD 28 induces specific intracellular signalling and leads to growth retardation and apoptosis via a p 56 ( lck ) dependent mechanism . ^^^ In parallel experiments with the p 56 ( lck ) negative Jurkat mutant cell , JCaM1 . 6 , cross linking neither influenced cell signalling nor cellular growth functions , indicating a cardinal role of the src kinases in signal transduction via MHC 1 , CD 2 and CD 28 molecules . ^^^ |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| The Lck tyrosine kinase is involved in signaling by T cell surface receptors such as TCR / CD3 , CD 2 , and CD 28 . ^^^ Using JCaM1 . 6 cells , we have demonstrated that Lck is required for CD 2 mediated phosphorylation of Dok proteins . ^^^ |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| TCR but not CD 2 triggered IL 2 production is p 56 ( lck ) dependent . ^^^ |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| While CD 2 induced p 62 ( dok ) tyrosine phosphorylation was independent of both the p36 / 38 membrane adapter protein linker of activated T cells ( LAT ) and the ZAP70 / Syk family of kinases , it was dependent upon the Src family of kinases including Lck and Fyn . ^^^ |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| Stimulation of tyrosine phosphorylation and activation of ITK by the T cell antigen receptor , CD 28 and CD 2 requires the presence of the Src family kinase Lck in T cells . ^^^ |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| NMR analysis shows that the Fyn but not the Lck tyrosine kinase SH 3 domain competes with CD2BP2 GYF domain binding to the same CD 2 proline rich sequence in vitro . ^^^ |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| Examination of protein kinases associating with these antigens showed that paradoxically , CD 2 coprecipitated the highest amount of Lck and Fyn . ^^^ CD 6 also associated with Lck , Fyn , and ZAP 70 , although at lower levels but additionally coprecipitated the Tec family kinase Itk , which is absent from CD 2 , CD 5 , and TCR complexes . ^^^ |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| They hypothesize that the key event in T cell activation is the formation of multimolecular complexes consisting of the TCR and several other polypeptides , including CD 4 or CD 8 , CD 2 , CD 5 and the associated tyrosine kinases p 59 ( fyn ) and p 56 ( lck ) . . ^^^ |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| We show that the coreceptor CD 2 , the adaptor protein LAT , and tyrosine kinase Lck cocluster in discrete microdomains in the plasma membrane of signaling T cells . ^^^ |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|