| Interacting proteins: P06702 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06702 and P13569 |
Pubmed |
SVM Score :0.0 |
| Application of this approach as a means of identifying biomarkers was demonstrated for cystic fibrosis ( CF ) lung disease by isolation and identification of inflammatory associated autoantigens , including myeloperoxidase and calgranulin B from sputum of subjects with CF . ^^^ |
|
| Interacting proteins: P06702 and P13569 |
Pubmed |
SVM Score :0.0 |
| Macrophage chemotactic factor ( CF ) and macrophage migration inhibition factor ( MIF ) were produced by day 3 of infection in spleen cell cultures stimulated with HSV 2 , but not with control antigen , i . e . 1 day before the cells are active in adoptive transfer . ^^^ The cells from 6 day immune mice active in adoptive transfer and CF and MIF production were found to be Thy 1+ , Ig and Lyt 2 . ^^^ |
|
| Interacting proteins: P06702 and P13569 |
Pubmed |
SVM Score :0.0 |
| The CF antigen has been shown to consist of the two calcium binding proteins MRP 8 and MRP 14 . ^^^ In the present study we investigate first whether elevated plasma titers of MRP 8 and MRP 14 are linked to the primary defect of CF or are rather a result of chronic airway inflammation ; and second , whether the known complexes of these proteins may have in vivo relevance during inflammation . ^^^ By employing the ELISA technique we measured MRP 8 and MRP 14 levels in the plasma of patients suffering from CF or nonspecific chronic bronchitis ( CB ) and of healthy controls , in sputum of CF and CB patients , and in saliva of CF patients and healthy controls , respectively . ^^^ MRP 8 and MRP 14 both were found in high amounts at similar concentrations in sputum of CF and CB patients and , to a lesser extent , in saliva of CF patients and healthy donors . ^^^ From this we conclude that the elevated plasma levels of MRP 8 and MRP 14 in CF and CB are the result of inflammatory processes . ^^^ |
|
| Interacting proteins: P06702 and P13569 |
Pubmed |
SVM Score :0.0 |
| We have demonstrated for the first time , by Northern analysis , the presence of the S 100 calcium binding proteins MRP 8 ( also called `` cystic fibrosis protein ' ' ) and MRP 14 mRNAs in cultured human tracheal gland cells , obtained from normal and cystic fibrosis ( CF ) patients . ^^^ Increased coexpression of CFTR and S 100 calcium binding proteins MRP 8 and MRP 14 mRNAs in cystic fibrosis human tracheal gland cells . ^^^ These results allow us to assume a potential pretranslational regulation of MRP 8 and MRP 14 gene expression related to the presence of a mutated CFTR gene . . ^^^ |
|
| Interacting proteins: P06702 and P13569 |
Pubmed |
SVM Score :0.0 |
| A complex of two calcium binding proteins , MRP 8 [ also called cystic fibrosis ( CF ) antigen ] and MRP 14 , proteins known to be expressed in cells of myeloid origin , has been shown to be present in higher amounts in the serum of CF patients and heterozygotes compared with normal subjects . ^^^ Moreover , using immunocytochemical methods , we showed that the localization of MRP 8 and MRP 14 on the plasma membrane seems to be restricted to the cells expressing a cystic fibrosis transmembrane conductance regulator ( CFTR ) wild type protein such as CAPAN 1 cells and CFPAC 1 cells transfected with a plasmid containing the nonmutated CFTR gene ( CFPAC PLJ CFTR 6 cells ) . ^^^ |
|
| Interacting proteins: P06702 and P13569 |
Pubmed |
SVM Score :0.0 |
| The serum protein cystic fibrosis associated antigen ( CFAG ) , present at elevated levels in CF homozygotes and heterozygotes , is now known to consist of two distinct but related subunits ( calgranulins A ( CAGA ) and B ( CAGB ) ) . ^^^ |
|
| Interacting proteins: P06702 and P13569 |
Pubmed |
SVM Score :0.0 |
| Sera that had been tested with use of complement fixation ( CF ) and microimmunofluorescence ( MIF ) tests were examined with ELISA . ^^^ ELISA was more sensitive than the CF test and as sensitive as the MIF test . ^^^ |
|
| Interacting proteins: P06702 and P13569 |
Pubmed |
SVM Score :0.0 |
| ELISA was thus less reliable than either CF or MIF for detecting antibody rises in paired infant sera , particularly where maternally acquired antibody remained in the acute serum . ^^^ |
|
| Interacting proteins: P06702 and P13569 |
Pubmed |
SVM Score :0.0 |
| Forty eight hours after skin testing and just before MIF and LT assays , blood was obtained for complement fixing ( CF ) antibody titers and the animals were autopsied to assess the extent of fungal dissemination . ^^^ Although this overall immunological profile is consistent with the disease in humans , there was not a direct correlation between CF antibody titer and dissemination to the liver or spleen , nor was there an inverse correlation between CF antibody titers and skin test or MIF responses . ^^^ |
|
| Interacting proteins: P06702 and P13569 |
Pubmed |
SVM Score :0.0 |
| Antibody responses to C . psittaci were demonstrated by the MIF test in all 36 CF positive patients . ^^^ The MIF test also detected antibody responses to C . psittaci in 12 patients ( 15 % of the total ) whose sera were negative or anticomplementary in the CF test . ^^^ About 30 % of the study patients ( 23 of 78 ) showed no serologic evidence of either C . psittaci or C . pneumoniae infection by both the CF and the MIF tests . ^^^ |
|
| Interacting proteins: P06702 and P13569 |
Pubmed |
SVM Score :0.0 |
| We performed a retrospective survey for antibody to Chlamydia pneumoniae , by the microimmunofluorescence ( MIF ) test , on 120 sera that were previously determined to be positive for antibody to ornithosis antigen by the complement fixation ( CF ) test . ^^^ There was no association between the CF titer to ornithosis antigen and the respective IgG or IgM MIF antibody titers . ^^^ We propose that , in those laboratories routinely using the CF test , sera found to be positive for ornithosis antigen should be further tested by the MIF in order to clarify , both from an epidemiologic and clinical perspective , whether these patients are also serologically positive for C . pneumoniae . . ^^^ |
|
| Interacting proteins: P06702 and P13569 |
Pubmed |
SVM Score :0.0 |
| Serological diagnosis of a C . pneumoniae infection was determined by the microimmunofluorescence ( MIF ) test , the complement fixation ( CF ) test , and recombinant lipopolysaccharide ( LPS ) enzyme linked immunosorbent assay ( ELISA ; referred to as the rDNA LPS ELISA ) . ^^^ Generally , the sensitivities of the rDNA LPS ELISA and MIF were comparable , while the sensitivity of the CF test was shown to be very low . ^^^ |
|
| Interacting proteins: P06702 and P13569 |
Pubmed |
SVM Score :0.0 |
| Comparison of four serological tests ( complement fixation ( CF ) test , microagglutination ( MA ) test , microimmunofluorescence ( MIF ) test , and enzyme linked immunosorbent assay ( ELISA ) ) for detection of post infection antibody response in human and animal sera revealed a low sensitivity of the CF test with acute Q fever human , goat and sheep sera but not with chronic Q fever human sera and sera of aborting cows . ^^^ |
|
| Interacting proteins: P06702 and P13569 |
Pubmed |
SVM Score :0.0 |
| These tests were the complement fixation ( CF ) test , the microimmunofluorescence ( MIF ) test , a recombinant enzyme immunoassay ( rEIA ) ( Medac ) based on a recombinant lipopolysaccharide of chlamydia and measuring antibodies to a common chlamydial antigen , and two tests that utilize preparations of C . pneumoniae organisms , the SeroCp EIA ( Savyon ) ( with preserved lipopolysaccharide ) and the LOY EIA ( Labsystems ) ( without this antigen ) . ^^^ Using an expanded `` gold standard , ' ' i . e . , the presence of significant reactions in at least two tests , the sensitivity of the CF test was 69 % , that of the MIF test was 88 % , that of the rEIA was 89 % , that of the LOY EIA was 96 % , and that of the SeroCp EIA was 92 % . ^^^ |
|
| Interacting proteins: P06702 and P13569 |
Pubmed |
SVM Score :0.0 |
| The diagnosis of chlamydial infection was based on an antibody response measured by complement fixation ( CF ) , by enzyme immunoassay ( EIA ; IgG or IgM ) or by microimmunofluorescence ( MIF ; IgG or IgM ) , and the diagnosis of mycoplasmal infection on CF alone . ^^^ |
|
| Interacting proteins: P06702 and P13569 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVES : To assess whether a novel functional MIF polymorphism was associated with clinical prognosis in a patient cohort with chronic gram negative infection , namely cystic fibrosis ( CF ) . ^^^ In patients with CF , the MIF 5 ( + ) group had a decreased incidence of Pseudomonas aeruginosa colonization ( odds ratio , 0 . 25 ; 95 % confidence interval , 0 . 09 0 . 65 ; p = 0 . 004 ) and a significant reduction in the risk of pancreatic insufficiency ( odds ratio , 0 . 27 ; 95 % confidence interval , 0 . 07 1 . 0 ; p = 0 . 05 ) . ^^^ CONCLUSIONS : The results support the concept of a regulatory role for MIF in CF . . ^^^ |
|
| Interacting proteins: P06702 and P13569 |
Pubmed |
SVM Score :0.0 |
| The CF , SV and HR were recovered by 81 + / 11 % , 104 + / 20 % and 78 + / 7 % when using Pra C and 86 + / 11 % , 106 + / 25 % and 82 + / 11 % , respectively , when using Nif . ^^^ |
|
| Interacting proteins: P06702 and P13569 |
Pubmed |
SVM Score :0.0 |
| From the isolated working hearts of prevention and regressive groups , it was found that AP , LVSP , +dp / dtmax , dp / dtmax , CF , and CI values of m Nif and Nif groups increased higher than that of LVH group , but LVEDP and T was lower in m Nif and Nif groups . ^^^ |
|
| Interacting proteins: P06702 and P13569 |
Pubmed |
SVM Score :0.0 |
| METHODS : The lungs of 20 day old congenic C57BL / 6 ( before any overt signs of inflammation ) and BALB / c CF mouse lines maintained in sterile environments were investigated for distinctions in the neutrophil chemokines S100A8 and S100A9 by quantitative RT PCR and RNA in situ hybridization , that were then correlated to neutrophil numbers . ^^^ RESULTS : The lungs of C57BL / 6 CF mice had spontaneous and significant elevation of both neutrophil chemokines S100A8 and S100A9 and a corresponding increase in neutrophils , in the absence of detectable pathogens . ^^^ In contrast , BALB / c CF mouse lungs maintained under identical conditions , had similar elevations of S100A9 expression and resident neutrophil numbers , but diverged in having normal levels of S100A8 . ^^^ CONCLUSION : The results indicate early and spontaneous lung inflammation in CF mice , whose progression corresponds to increased expression of both S100A8 and S100A9 , but not S100A9 alone . ^^^ Moreover , since both C57BL / 6 and BALB / c CF lungs were maintained under identical conditions and had similar elevations in S100A9 and neutrophils , the higher S100A8 expression in the former ( or suppression in latter ) is a result of secondary genetic influences rather than environment or differential infection . . ^^^ |
|
| Interacting proteins: P06702 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06702 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06702 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06702 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06702 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06702 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
|