Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.82147635
MRP 14 , another S 100 protein generally coordinately expressed with human MRP 8 , was not induced by LPS under the same conditions . 0.82147635^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.87579389
Although S100A9 associates with S100A8 in neutrophils and abnormally differentiated keratinocytes ( human psoriasis ) , in this study it was not coinduced with keratinocyte S100A8 . 0.87579389^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
These include psoriasin ; calgranulin B , also known as MRP 14 , L 1 , or calprotectin ; calgranulin A or MRP 8 ; and cystatin A or stefin A . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Psoriasin is not related to other proteins that migrate closely in 2D gels ( MRP 14 , also known as calgranulin B , L 1 and calprotectin ; MRP 8 , or calgranulin A and cystatin A or stefin A ) , and bears no significant sequence homology with any other protein of known primary structure . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Calgranulin A ( CAGA ) and calgranulin B ( CAGB ) are two S 100 like calcium binding proteins that in human , bovine and mouse granulocytes are associated into a heterocomplex . ^^^ Calgranulin A ( CAGA ) and calgranulin B ( CAGB ) are two S 100 like calcium binding proteins that in human , bovine and mouse granulocytes are associated into a heterocomplex . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
These includes calgranulin A , calgranulin B , neutrophil activating protein ( ENA 78 ) , heat shock protein 27 , integrin beta 1 , integrin beta 4 , cyclin dependent kinase inhibitor 1A ( p 21 ) , interleukin 8 and tyrosine protein kinase receptor ( RET ) . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
The resulting tryptic peptides were sequenced by tandem mass spectrometry , and based on the recovered partial amino acid sequences , three of the tumor specific protein markers were identified as calgranulin A ( S100A8 ) , calgranulin B ( S100A9 ) and calgizzarin ( S100A11 ) . . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Using a phosphorimager the analysis of the cDNA arrays revealed nine cytokines which were overexpressed compared with the reference tissue ( Calgranulin A , Calgranulin B , IL 10 , MCP 1 , RANTES , TFDG 1 , TNFR 2 , RAI , THYB 10 ) . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
These included genes involved in cell proliferation and differentiation ( eg , calgranulin A , calgranulin B , psoriasin , thymosin beta 10 ) and cell invasion ( eg , cathepsin C , cathepsin D , cathepsin H ) . ^^^ Analyses by means of reverse transcription polymerase chain reaction showed enhanced expression of several genes including calgranulin A , calgranulin B , psoriasin , thymosin beta 10 , cathepsin C , cathepsin D , and cathepsin H in cholesteatoma , supporting the findings from the gene array . ^^^ In addition , it was verified by immunohistochemical analysis that the expressions of Calgranulin A , Calgranulin B , and Cathepsin D were mainly located in cholesteatoma epithelium . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Two protein spots were identified as calgranulin A and three spots as calgranulin B . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Two differentially expressed proteins ( P = 3 . 34 10 10 ( 5 ) and 4 . 6 10 10 ( 5 ) ) were isolated by two dimensional gel electrophoresis and identified as S100A8 ( calgranulin A ) and S100A9 ( calgranulin B ) by in gel proteolytic digestion , peptide mapping , tandem mass spectrometry analysis , and immunodepletion assay . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Significantly , levels of CRP , S100A8 ( calgranulin A ) , S100A9 ( calgranulin B ) , and S100A12 ( calgranulin C ) proteins were also elevated in the serum of patients with erosive disease compared with patients with nonerosive RA or healthy individuals . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
The concentrations of neutrophil defensins 1 and 2 , S100A8 ( calgranulin A ) , and S100A9 ( calgranulin B ) were elevated in BALFs of smokers with COPD when compared to asymptomatic smokers . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Gene expressions regulated by IL 22 should enhance antimicrobial defense [ psoriasin ( S100A7 ) , calgranulin A ( S100A8 ) , calgranulin B ( S100A9 ) ] , inhibit cellular differentiation ( e . g . , profilaggrin , keratins 1 and 10 , kallikrein 7 ) , and increase cellular mobility [ e . g . , matrix metalloproteinease 1 ( MMP 1 , collagenase 1 ) , MMP 3 ( stromelysin 1 ) , desmocollin 1 ] . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
A subset of amniotic fluid was also tested for leukemia inhibitory factor , angiogenin , and migration inhibitory factor related protein 8 and migration inhibitory factor related protein 14 . ^^^ RESULTS : Median concentrations of interleukin 1beta , tumor necrosis factor alpha , interleukin 4 , Clara cell protein 16 , leptin , and angiogenin were increased in amniotic fluid from twins ; median levels of the 70 kDa heat shock protein , leukemia inhibitory factor , migration inhibitory factor related protein 8 , and migration inhibitory factor related protein 14 were highest in amniotic fluid from singletons ( P < . 001 ) . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
The expression of the heterodimeric complex of the calcium binding proteins MRP 8 and MRP 14 was investigated in various inflammatory dermatoses using immunohistochemical staining with the monoclonal antibody 27E10 . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Mouse MRP 8 and MRP 14 , two intracellular calcium binding proteins associated with the development of the myeloid lineage . ^^^ MRP 8 and MRP 14 are two S 100 like calcium binding proteins of unknown function , associated with numbers of human inflammatory disorders . ^^^ We report here the cloning of mouse MRP 8 and MRP 14 and their pattern of expression during hematopoiesis . ^^^ Mouse MRP 8 and MRP 14 proteins share 59 % identity with their human counterparts , but they are more divergent than the other members of the S 100 protein family . ^^^ In adult mouse , we identified MRP 8 and MRP 14 proteins in immature myeloid cells of the bone marrow , myeloid cells in the splenic red pulp and marginal zone , in addition to monocytes and blood neutrophils . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
The calcium binding proteins MRP 8 and MRP 14 form a membrane associated heterodimer in a subset of monocytes / macrophages present in acute but absent in chronic inflammatory lesions . ^^^ This report shows that the 27E10 antigen is formed by noncovalent association of the two Ca ( 2+ ) binding proteins MRP 8 and MRP 14 which belong to the S 100 protein family . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Two calcium binding proteins , MRP 8 and MRP 14 , are specifically synthesized in human myeloid cells . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Complex pattern of the myelo monocytic differentiation antigens MRP 8 and MRP 14 during chronic airway inflammation . ^^^ The CF antigen has been shown to consist of the two calcium binding proteins MRP 8 and MRP 14 . ^^^ In the present study we investigate first whether elevated plasma titers of MRP 8 and MRP 14 are linked to the primary defect of CF or are rather a result of chronic airway inflammation ; and second , whether the known complexes of these proteins may have in vivo relevance during inflammation . ^^^ By employing the ELISA technique we measured MRP 8 and MRP 14 levels in the plasma of patients suffering from CF or nonspecific chronic bronchitis ( CB ) and of healthy controls , in sputum of CF and CB patients , and in saliva of CF patients and healthy controls , respectively . ^^^ MRP 8 and MRP 14 both were found in high amounts at similar concentrations in sputum of CF and CB patients and , to a lesser extent , in saliva of CF patients and healthy donors . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
The calcium binding proteins MRP 8 and MRP 14 in acute and chronic inflammation . ^^^ In the latter situation monocytes either express MRP 8 or MRP 14 and not both as in acute inflammation . ^^^ In all inflammation models tested so far the cells arriving first at the lesion were MRP 8 and MRP 14 positive . ^^^ MRP 8 and MRP 14 thus represent two novel molecular parameters of the early events of inflammatory reactions which reveal interesting aspects for the pathomechanism of chronic inflammatory reactions . . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
The markers MRP 8 and MRP 14 typical of immature macrophages , were found in all patients with osteoarthritis and in some ( 4 / 9 and 8 / 9 respectively ) of those with THA loosening . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Bovine neutrophil p 23 and p 7 presented large primary structure homologies with two human proteins , MRP 14 and MRP 8 , which are expressed in large amounts in macrophages under conditions of chronic inflammation . ^^^ In addition , p 23 and p 7 cross reacted with monoclonal antibodies specific of MRP 14 and MRP 8 . ^^^ Bovine p 23 and p 7 bound Ca2+ , and their amino acid sequences contained two Ca ( 2+ ) binding domains per protein , largely identical to those of human MRP 14 and MRP 8 . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Expression of the calcium binding proteins MRP 8 and MRP 14 by early infiltrating cells in experimental contact dermatitis . ^^^ We recently described the expression of MRP 8 and MRP 14 , two calcium binding proteins of the S 100 family , by these cells during inflammatory activation . ^^^ Therefore we performed immunohistochemical studies on the distribution of MRP 8 and MRP 14 positive cells during experimentally induced allergic ( ACD ) and irritant contact dermatitis ( ICD ) . ^^^ The expression of MRP 8 and MRP 14 in both forms of dermatitis correlated with the early influx of macrophages and with the cell density of the infiltrate . ^^^ Also the percentage of MRP 8 and MRP 14 positive cells in the infiltrate during ACD or ICD was higher in the more intense inflammatory reaction of BALB / c mice compared to C57Bl / 6 mice . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
OH ) 2 vitamin D 3 enhances expression of the genes encoding Ca ( 2+ ) binding proteins MRP 8 and MRP 14 . ^^^ Because 1 alpha , 25 dihydroxyvitamin D 3 [ 1 , 25 ( OH ) 2D3 ] induces myeloid cell differentiation and expression of other S 100 class calcium binding proteins , we examined the effects of 1 , 25 ( OH ) 2D3 on MRP mRNA levels in human U 937 histiocytic lymphoma cells . 1 , 25 ( OH ) 2D3 increased MRP 8 and MRP 14 mRNA levels in a time and dose dependent manner . ^^^ Other differentiating agents , dimethyl sulfoxide , retinoic acid , and dexamethasone , also increased levels of MRP 8 and MRP 14 mRNA . ^^^ Phorbol myristate acetate enhanced MRP 14 mRNA levels to a greater extent than MRP 8 mRNA levels , suggesting differential regulation of MRP gene expression by protein kinase C . ^^^ Expression of the MRP 8 and MRP 14 genes may be important for differentiation of myeloid cells . . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Calcium dependent complex assembly of the myeloic differentiation proteins MRP 8 and MRP 14 . ^^^ MRP 8 and MRP 14 are calcium binding proteins belonging to the S 100 protein family which have been shown to be associated with specific stages of myeloic / monocytic cell differentiation . ^^^ Complex formation has also been observed in preliminary experiments for MRP 8 and MRP 14 . ^^^ The two larger protein complexes ( 35 . 0 and 48 . 5 kDa ) were identified as [ MRP 8 ) 2 . ( MRP 14 ] trimer and [ MRP 8 ) 2 . ( MRP 14 ) 2 ) tetramer , respectively . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Differential expression of the calcium binding proteins MRP 8 and MRP 14 in granulomatous conditions : an immunohistochemical study . ^^^ MRP 14 and MRP 8 are well characterized calcium binding proteins present in myeloid cells and mononuclear phagocytes . ^^^ This study evaluates MRP 14 and MRP 8 expression in mononuclear phagocytes in various granulomatous conditions . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
The expression of c myc and two calcium binding proteins , MRP 8 and MRP 14 , has been analyzed in wild type and differentiation resistant HL 60 variants . ^^^ In HL R 5 cells , resistant to the induction of differentiation by retinoic acid but not DMSO , the characteristic c myc down regulation which is associated with HL 60 differentiation , as well as increased levels of MRP 8 and MRP 14 , is detectable only after DMSO treatment . ^^^ Neither agent can induce an increase in the level of either MRP 8 or MRP 14 in HL D 4 . ^^^ The resistance of HL D 4 cells to DMSO and retinoic acid , and the different effects of these agents on c myc RNA levels , despite their common effect on the expression of MRP 8 and MRP 14 , suggest that the two agents act through different pathways which coverage before the onset of myeloid differentiation in HL 60 cells . . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
A protein complex ( PC ) composed of the MRP 8 and MRP 14 proteins has previously been shown to be a specific inhibitor of casein kinase 1 and 2 . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
These proteins have relative molecular masses ( Mr ) of 8 , 000 and 14 , 000 and are variously referred to as the cystic fibrosis antigen , the L 1 light chain , MRP 8 or p 8 , and the L 1 heavy chain , MRP 14 or p 14 , respectively . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Monoclonal antibodies 27E10 , 25F9 , and RM3 / 1 , which react with different macrophage phenotypes , and antisera MRP 8 and MRP 14 , specific for proteins expressed on endothelial and monocyte cell surfaces in inflammation as well as markers for CD4+ and CD8+ T lymphocytes , were employed in an indirect immunoperoxidase staining technique . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Using antibodies directed against MRP 8 and its closely associated calcium binding protein , MRP 14 , we demonstrate here that cystic fibrosis protein purified from serum is a complex of the two proteins MRP 8 and MRP 14 . . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
They are therefore designated MIF related proteins or MRP 8 and MRP 14 according to their apparent molecular weights . ^^^ In acute inflammatory reactions , e . g . gingivitis , MRP 8 is never seen in the tissue , whereas MRP 14 is expressed by intravascular monocytes and perivascular macrophages . ^^^ From this it is concluded that MRP 8 and MRP 14 are expressed sequentially at defined stages of monocyte / macrophage differentiation and that dysregulation of this process in chronic inflammation is mirrored by the presence of MRP 8 positive macrophages in the tissue . . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
The expression of these proteins , referred to as MRP 8 and MRP 14 , is specific for cells of myeloid origin , namely granulocytes , monocytes and macrophages , and is observed in blood granulocytes and monocytes but not in normal tissue macrophages . ^^^ In acutely inflamed tissues , macrophages can express MRP 14 but not MRP 8 , and in chronic inflammations , such as primary chronic polyarthritis , infiltrate macrophages express both MRP 8 and MRP 14 . ^^^ Characterization of MRP 8 and MRP 14 could therefore be useful to the understanding of cellular processes induced in chronic inflammation . . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Two proteins , MRP 8 and MRP 14 , which are expressed in infiltrate macrophages during inflammatory reactions but not in normal tissue macrophages , have been characterized . ^^^ Here we report that MRP 8 and MRP 14 mRNAs are specifically expressed in human cells of myeloid origin and that their expression is regulated during monocyte macrophage and granulocyte differentiation . ^^^ To initiate the analysis of cis acting elements governing the tissue specific expression of the MRP genes , we cloned the human genes encoding MRP 8 and MRP 14 . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
The leucocyte L 1 protein : identity with the cystic fibrosis antigen and the calcium binding MRP 8 and MRP 14 macrophage components . ^^^ The partial amino acid sequence of L 1 protein light and heavy chains reveals an overall structure identical to the two macrophage proteins , MRP 8 and MRP 14 , deduced from the sequence of the cDNA encoding the polypeptides . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
MRP 8 , MRP 14 and their heterodimer MRP8 / 14 ( 27E10 antigen ) are myeloic related proteins which have been shown to have a major role in inflammatory and immunological responses . ^^^ MRP 8 , MRP 14 and their heterocomplex MRP8 / 14 were demonstrated in the majority of granulocytes and macrophages in tissues of patients with active UC . ^^^ No enhancement of serum levels were found for MRP 14 and MRP 8 alone , respectively . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
MAC 387 has been described to show a similar reaction pattern as antisera to a complex formed by the calcium binding proteins MRP 8 and MRP 14 . ^^^ Transfection of embryonic lung fibroblasts L 132 with either MRP 8 or MRP 14 cDNA revealed that MAC 387 reacts with MRP 14 but not MRP 8 . ^^^ This finding was confirmed by dot blot analysis of recombinant MRP 8 and MRP 14 . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Increased coexpression of CFTR and S 100 calcium binding proteins MRP 8 and MRP 14 mRNAs in cystic fibrosis human tracheal gland cells . ^^^ We have demonstrated for the first time , by Northern analysis , the presence of the S 100 calcium binding proteins MRP 8 ( also called `` cystic fibrosis protein ' ' ) and MRP 14 mRNAs in cultured human tracheal gland cells , obtained from normal and cystic fibrosis ( CF ) patients . ^^^ These results allow us to assume a potential pretranslational regulation of MRP 8 and MRP 14 gene expression related to the presence of a mutated CFTR gene . . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Expression and complex formation of S 100 like proteins MRP 8 and MRP 14 by macrophages during renal allograft rejection . ^^^ MRP 8 and MRP 14 , two S 100 like calcium binding proteins , are expressed during differentiation of monocytes / macrophages . ^^^ The present study was intended to investigate the molecular basis of macrophage heterogeneity with respect to expression and complex formation of MRP 8 and MRP 14 during acute and chronic rejection of renal allografts . ^^^ First , specificity of antisera and mAbs to be directed against MRP 8 , MRP 14 , or MRP8 / MRP14 heterodimers was determined by immunocytochemical and Western blot analyses of L 132 fibroblasts ( co ) transfected with MRP 8 and / or MRP 14 cDNA . ^^^ Then , immunohistochemical analysis of biopsy specimens obtained from kidney allografts after acute rejection was performed , revealing a parallel expression of MRP 8 and MRP 14 with coincident MRP8 / MRP14 heterodimer formation in infiltrating monocytes . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Increase of calcium levels in epithelial cells induces translocation of calcium binding proteins migration inhibitory factor related protein 8 ( MRP 8 ) and MRP 14 to keratin intermediate filaments . ^^^ Migration inhibitory factor related protein 8 ( MRP 8 ) and MRP 14 , two S 100 like Ca ( 2+ ) binding proteins , have been described in cells of the epithelial lineage where they are either expressed constitutively ( e . g . by mucosal squamous epithelium ) or induced during disease ( e . g . in keratinocytes during the course of psoriasis ) . ^^^ Migration inhibitory factor related protein 8 ( MRP 8 ) and MRP 14 , two S 100 like Ca ( 2+ ) binding proteins , have been described in cells of the epithelial lineage where they are either expressed constitutively ( e . g . by mucosal squamous epithelium ) or induced during disease ( e . g . in keratinocytes during the course of psoriasis ) . ^^^ Recent studies have provided evidence that S 100 like proteins may interact with cytoskeletal components ; we have therefore studied the biochemical properties and subcellular distribution of MRP 8 and MRP 14 in epithelial cells . ^^^ TR 146 human squamous carcinoma cells , which were found to express MRP 8 and MRP 14 in Northern and Western blot studies , were chosen for analysis . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Presence of MRP 8 and MRP 14 in pancreatic cell lines : differential expression and localization in CFPAC 1 cells . ^^^ A complex of two calcium binding proteins , MRP 8 [ also called cystic fibrosis ( CF ) antigen ] and MRP 14 , proteins known to be expressed in cells of myeloid origin , has been shown to be present in higher amounts in the serum of CF patients and heterozygotes compared with normal subjects . ^^^ Moreover , using immunocytochemical methods , we showed that the localization of MRP 8 and MRP 14 on the plasma membrane seems to be restricted to the cells expressing a cystic fibrosis transmembrane conductance regulator ( CFTR ) wild type protein such as CAPAN 1 cells and CFPAC 1 cells transfected with a plasmid containing the nonmutated CFTR gene ( CFPAC PLJ CFTR 6 cells ) . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
The 87 kDa receptor showed sequence homologies with lactoferrin and the 59 kDa receptor represented a complex of two Ca binding proteins MRP 8 and MRP 14 reportedly expressed only by cells of myeloid origin . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Heterodimers of the calcium binding proteins MRP 8 and MRP 14 are expressed on the surface of human monocytes upon adherence to fibronectin and collagen . ^^^ The 27E10 antigen is a heterodimer of MRP 8 and MRP 14 , two Ca ( 2+ ) binding proteins related to the S 100 protein family . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
A rabbit polyclonal antibody raised against myeloid related protein 8 ( MRP 8 ) , a protein of the S 100 family , recognized another S 100 protein ( MRP 14 ) as well as a protein of 6 . 5 kDa ( p 6 ) in the cytosol of resting neutrophils . p 6 was found to be a novel member of the S 100 family . ^^^ Both isoforms were also demonstrated in the cytosol of monocytes , but not in lymphocytes , as previously shown for MRP 8 and MRP 14 . ^^^ Only the major isoform bound radioactive Ca2+ , as also observed for MRP 8 , whereas the different variants of MRP 14 were all labelled . ^^^ This translocation pattern was similar to that observed with MRP 8 and MRP 14 . ^^^ In addition , p 6 , MRP 8 and MRP 14 were specifically associated with the cytoskeletal fraction of the membrane . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
MRP 8 and MRP 14 are myeloic related proteins expressed by most circulating and emigrated neutrophils and monocytes . ^^^ Patients being treated with Zidovudine showed no statistically significant variation in levels of 27E10 and its single components MRP 8 and MRP 14 compared with untreated patients . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Expression and complex assembly of calcium binding proteins MRP 8 and MRP 14 during differentiation of murine myelomonocytic cells . ^^^ In the present study we analyzed expression and biochemical properties of the two recently cloned calcium binding proteins MRP 8 and MRP 14 , both members of the S 100 family , in murine myelomonocytic cells . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
N terminal sequence analysis suggests that p7A is homologous to human and mouse MRP 8 and that p7B may be related to human and mouse MRP 14 , though some properties of the latter such as mobility on SDS PAGE were found to be different . ^^^ In addition , p7A and p7B could be resolved under native conditions , contrasting with the fact that human and mouse MRP 8 / MRP 14 form noncovalent complexes . . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
We studied the candidastatic activity of human neutrophils , neutrophil cytosol , and neutrophil derived `` calprotectin , ' ' a cytosolic protein complex comprised of two subunits , MRP 8 and MRP 14 . ^^^ After reversed phase HPLC , calprotectin fractions containing MRP 14 exhibited fungistatic activity , whereas fractions depleted of MRP 14 but enriched for MRP 8 lacked fungistatic activity . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Immunohistochemical demonstration of the calcium binding proteins MRP 8 and MRP 14 and their heterodimer ( 27E10 antigen ) in Crohn ' s disease . ^^^ Monospecific antibodies against the calcium binding proteins MRP 8 and MRP 14 and their heterodimer MRP8 / 14 ( epitope 27E10 ) were used to investigate immunohistochemically the distribution of these proteins in routinely processed small and large bowel tissues from patients with Crohn ' s disease . ^^^ MRP 8 , MRP 14 , and complex MRP8 / 14 were demonstrated in most granulocytes and macrophages in active Crohn ' s disease . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Expression of the calcium binding proteins MRP 8 and MRP 14 in monocytes is regulated by a calcium induced suppressor mechanism . ^^^ MRP 8 and MRP 14 are two calcium binding proteins of the S 100 family the expression of which is restricted to distinct stages of monocytic differentiation . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Ultrastructural localization of the S 100 like proteins MRP 8 and MRP 14 in monocytes is calcium dependent . ^^^ MRP 8 and MRP 14 are members of the S 100 family of Ca ( 2+ ) binding proteins and are expressed by granulocytes and monocytes . ^^^ Monospecific rabbit antisera against MRP 8 and MRP 14 and a monoclonal antibody ( moAb 27E10 ) , which exclusively recognizes the MRP8 / MRP14 heterodimer but not the monomers , were used in both immunoperoxidase / preembedding and immunogold / cryotechniques . ^^^ Comparing non stimulated monocytes with Ca2+ ionophore A 23187 treated cells , we could demonstrate that MRP 8 and MRP 14 associate with membrane and cytoskeletal structures in a Ca ( 2+ ) dependent manner . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
We further selected several genes on basis of the results from gene subtraction between cDNA libraries from neutrophils at an early ( 5 h ) and at a late ( 24 h ) stage of casein induced acute peritonitis in rabbits , i . e . immune activation gene 2 ( Act 2 ) , migration inhibitory factor related protein 8 ( MRP 8 ) , MRP 14 , gamma actin , and formyl methionyl leucyl phenylalanine receptor ( fMLP R ) , and ferritin light ( L ) chain . ^^^ Pattern 1 : mRNAs of MRP 8 , MRP 14 , and gamma actin were constitutively expressed in blood neutrophils , and increased rapidly after emigration into inflammatory sites . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Rat migration inhibitory factor related protein 8 ( MRP 8 ) and MRP 14 were identified during screening of a subtracted cDNA library generated to identify differences in gene expression between LEW / N and F344 / N rats . ^^^ The predicted amino acid sequence of rat MRP 8 and MRP 14 is 60 80 % identical with that from human and mouse . ^^^ These differences suggest a role for MRP 8 and MRP 14 in susceptibility to SCW induced chronic disease . . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Expression of calcium binding proteins MRP 8 and MRP 14 is associated with distinct monocytic differentiation pathways in HL 60 cells . ^^^ MRP 8 and MRP 14 are two calcium binding proteins of the S 100 family which are expressed during distinct stages of monocytic maturation . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
MRP 8 and MRP 14 , S 100 like proteins associated with myeloid differentiation , are translocated to plasma membrane and intermediate filaments in a calcium dependent manner . ^^^ MRP 8 and MRP 14 are two Ca ( 2+ ) binding proteins of the S 100 family expressed by myelomonocytic cells . ^^^ Analysis of MRP transfected L 132 cells showed that the MRP 8 as well as the MRP 14 component of the MRP8 / MRP14 complex may independently bind to membrane and cytoskeletal structures . ^^^ Our data indicate that , in analogy to other S 100 like proteins , MRP 8 and MRP 14 play a role in Ca ( 2+ ) dependent cytoskeletal membrane interactions . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Regulated expression of the MRP 8 and MRP 14 genes in human promyelocyticleukemic HL 60 cells treated with the differentiation inducing agents mycophenolic acid and 1 alpha , 25 dihydroxyvitamin D 3 . ^^^ The calcium binding proteins MRP 8 and MRP 14 present in mature monomyelocytic cells are induced during differentiation and may mediate the growth arrest in differentiating HL 60 cells . ^^^ We determined the levels of a protein complex ( PC ) containing MRP 8 and MRP 14 and investigated the mechanism by which the genes encoding these proteins are regulated in HL 60 cells treated with the differentiation inducing agent mycophenolic acid ( MPA ) . ^^^ While the PC was barely detectable in untreated cells , MPA treatment resulted in elevated levels of the PC which were maximal at 3 4 days and were found to directly parallel gains in the steady state levels of MRP 8 and MRP 14 mRNA . ^^^ Transcription studies with the use of nuclear run on experiments revealed increased transcription initiation at the MRP 8 and MRP 14 promoters after MPA treatment . 1 alpha , 25 Dihydroxyvitamin D 3 , which induces HL 60 cell differentiation by another mechanism , was also found to increase transcription initiation at the MRP 8 and MRP 14 promoters . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
MRP 8 and MRP 14 , two abundant Ca ( 2+ ) binding proteins of neutrophils and monocytes . ^^^ Two calcium binding proteins , named migration inhibitory factor related proteins 8 ( MRP 8 ) and MRP 14 , are primarily expressed by circulating human neutrophils and monocytes . ^^^ Both MRP 8 and MRP 14 display features characteristic of members of the S 100 family of calcium binding proteins . ^^^ Some of these features predict functions for MRP 8 and MRP 14 but to date an exact and well defined function remains elusive . ^^^ Here we review the available information and highlight evidence that suggests the function of MRP 8 and MRP 14 may be associated with both monocyte and neutrophil activation and the accumulation of these cells in inflammatory sites . . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Appearance of nuclear factors that interact with genes for myeloid calcium binding proteins ( MRP 8 and MRP 14 ) in differentiated HL 60 cells . ^^^ Myeloid calcium binding proteins MRP 8 and MRP 14 were induced , and their genes were coordinately expressed , during differentiation of human leukemia HL 60 cells into macrophage like cells after treatment with 1 , 25 dihydroxyvitamin D 3 ( VD 3 ) . ^^^ Both MRP 8 and MRP 14 mRNAs appeared on the day after VD 3 treatment . ^^^ Nuclear factors that interact with the 5 ' upstream regions of MRP 8 and MRP 14 genes were studied with gel mobility shift assays . ^^^ These factors may be involved in the expression of MRP 8 and MRP 14 genes in VD 3 differentiated HL 60 cells . . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
The two L 1 chains ( L1H and L1L ) are referred to by a bewildering collection of names , various authors having different preferences ( MRP 8 and MRP 14 ; CFA or calgranulin A and B ) . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Immunohistochemical distribution and serum levels of the Ca ( 2+ ) binding proteins MRP 8 , MRP 14 and their heterodimeric form MRP8 / 14 in Crohn ' s disease . ^^^ In previous histochemical studies the distribution of the two Ca ( 2+ ) binding proteins MRP 8 and MRP 14 as well as their heterocomplex MRP8 / 14 has been demonstrated in different inflammatory diseases . ^^^ Monoclonal antibodies against MRP 8 and MRP 14 and their heterodimer MRP8 / 14 ( 27E10 epitope ) were used to investigate immunohistochemically the distribution of these proteins in routinely processed small and large bowel tissues from patients with Crohn ' s disease ( CD ) . ^^^ In our immunohistochemical study , MRP 8 , MRP 14 and heterocomplex MRP8 / 14 were demonstrated in the majority of granulocytes and macrophages in active CD . ^^^ No correlations were found for level of MRP 14 and MRP 8 alone , respectively . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
MRP 8 and MRP 14 are two Ca ( 2+ ) binding proteins expressed in myelomonocytic cells . ^^^ Complexes of MRP 8 and MRP 14 colocalize with membranes and intermediate filaments in a Ca ( 2+ ) dependent manner . ^^^ MRP 14 , unlike MRP 8 , exists in two isoforms , the smaller of which ( MRP 14 ' ) has been shown to lack the first four amino acids ; both MRP 14 and MRP 14 ' are also present as phosphorylated forms . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Immunohistochemical investigations were performed with the antibodies Ki M1P , 27E10 , MRP 14 , MRP 8 and 25F9 . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Phosphorylation of myeloid related proteins MRP 14 and MRP 8 during human neutrophil activation . ^^^ The myeloid related proteins MRP 14 and MRP 8 and also p 6 , three calcium binding proteins of the S 100 family , translocate to the membrane during human neutrophil activation with stimuli known to require extracellular calcium for activity . ^^^ Cgp 41251 , an inhibitor of protein kinase C ( PKC ) , completely inhibited the phosphorylation of MRP 8 , and decreased cytosolic MRP 14 phosphorylation . ^^^ This resulted in translocation of 18 % + / 5 % of phosphorylated MRP 14 and 19 % + / 1 % of only nonphosphorylated MRP 8 . ^^^ However , upon inhibition of PKC , translocation of MRP 14 and MRP 8 was increased up to 38 % + / 7 % and 34 % + / 3 % respectively . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Because quartz treated BALB / c , but not DBA / 2 mice , showed a persistent expression of the macrophage differentiation markers MRP 8 and MRP 14 , phenotypically the observed strain difference in silicotic responsiveness seems to be expressed at the level of macrophages . . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Functional chemotactic factor CP 10 and MRP 14 are abundant in murine abscesses . ^^^ We report the presence of large amounts of two murine S 100 proteins , CP 10 and migration inhibition factor related protein 14 ( MRP 14 ) , in abscesses as demonstrated by immunohistochemistry and measured by enzyme linked immunosorbent assay and Western blotting ( immunoblotting ) . ^^^ High levels of CP 10 ( 7 . 7 + / 1 mg / ml ) and MRP 14 ( 5 . 5 + / 1 mg / ml ) were found throughout the time course of abscess development from early acute phase lesions , which are predominantly neutrophilic , to late chronic phase lesions , which contained more mononuclear cells . ^^^ Approximately one third of these amounts occurred as monomers ( 2 . 0 mg / ml for MRP 14 and 2 . 2 mg / ml for CP 10 ) . ^^^ CP 10 MRP 14 complexes were present in abscess fluid , and the proteins were immunoabsorbed together . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
MRP 14 bound more 85Zn2+ than the same amounts of the 10 kDa chemotactic protein ( CP 10 ) or S 100 beta . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Upregulated proteins included keratins ( keratins 6 , 10 / 11 , 14 and 16 ) , members of the S 100 protein family psoriasin , MRP 8 , MRP 14 and S100c ) , actin binding proteins ( gelsolin and tropomyosin 9220 ) , annexins ( annexins 4 and 8 ) , hsp 28 , the fatty acid binding protein 5 ( FABP 5 ) , the squamous cell carcinoma ( SCC ) antigen , members of the 14 3 3 family , involucrin , E cadherin , cystatin A , desmoglein and integrins alpha 2 and beta 1 , as well as several proteins of as yet unknown identity . ^^^ The highest upregulated proteins corresponded to psoriasin ( 124 . 0 times ) , MRP 8 ( 42 . 4 times ) , MRP 14 ( 14 . 9 times ) , tropomyosin 9220 ( 11 . 5 times ) , involucrin ( 11 . 1 times ) , and FABP 5 ( 9 . 1 times ) . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
We previously reported that levels of myeloid calcium binding protein ( MRP 8 and MRP 14 ) were elevated in the serum of patients with connective tissue diseases . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Mac 387 , a murine mAb , was previously described to detect a complex form of MRP 14 and MRP 8 , two calcium binding proteins of the S 100 family , but recent experiments suggested that Mac 387 recognized only MRP 14 . ^^^ Using two dimensional polyacrylamide gel electrophoresis and the very sensitive enhanced chemiluminescence detection system , the immunoreactivity of Mac 387 was compared with that of a polyclonal antibody raised against purified MRP 8 , but cross reacting with MRP 14 and p 6 , a novel S 100 protein . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
As a marker for the activation of these `` brain macrophages , ' ' we have used the macrophage inhibitor factor related proteins MRP 8 and MRP 14 , which belong to the calcium binding S 100 protein family . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Immunohistochemical preparations of lung specimens were tested with the antibodies MRP 8 , MRP 14 , 27E10 and 25F9 in cases of protracted asphyxiation ( n = 8 ) and in `` control ' ' groups ( hanging : n = 6 ; peracute deaths : n = 9 ) . ^^^ MRP 8 and MRP 14 interstitial cell counts showed a doubling in protracted asphyxiation . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Identification of the mouse calcium binding proteins , MRP 8 and MRP 14 , in Schistosoma mansoni induced granulomas : biochemical and functional characterization . ^^^ Immunohistochemically , cells expressing MRP 8 and MRP 14 considerably increased in different murine tissues after Schistosoma mansoni infection and concentrated in liver around the dilated blood vessels and at the edge of granulomas . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
A heterocomplex formed by the calcium binding proteins MRP 8 ( S100A8 ) and MRP 14 ( S100A9 ) binds unsaturated fatty acids with high affinity . ^^^ We show that unsaturated fatty acids ( FAs ) bind reversibly and with high affinity to a heterocomplex of 34 kDa ( FA p 34 ) formed by the non covalent association of two calcium binding proteins of the S 100 family : MRP 8 ( S100A8 ) and MRP 14 ( S100A9 ) . ^^^ The individual subunits MRP 8 and MRP 14 show no binding properties for fatty acids , whereas a p 34 complex reconstituted in vitro by the recombinant molecules exhibits binding properties , suggesting that the fatty acid binding site of FA p 34 is created through heterocomplex formation . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
We now demonstrate the specific , energy dependent release of MRP 8 and MRP 14 by human monocytes after activation of protein kinase C . ^^^ This secretory process is not blocked by inhibitors of vesicular traffic through the endoplasmic reticulum and Golgi , and comparative studies on tumor necrosis factor alpha and interleukin 1beta indicate that MRP 8 and MRP 14 follow neither the classical nor the interleukin 1 like alternative route of secretion . ^^^ Release of MRP 8 and MRP 14 is associated with down regulation of their de novo synthesis , suggesting that extracellular signaling via MRP8 / MRP14 is restricted to distinct differentiation stages of monocytes . ^^^ Our data provide evidence that the S 100 proteins MRP 8 and MRP 14 are secreted after activation of protein kinase C via a novel pathway requiring an intact microtubule network . . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
The heterodimer of the Ca2+ binding proteins MRP 8 and MRP 14 binds to arachidonic acid . ^^^ The S 100 proteins MRP 8 and MRP 14 have been shown to be expressed by myeloid cells during inflammatory reactions . ^^^ Since the majority of S 100 proteins exhibit their biological activity when associated as complex it was investigated whether murine MRP 8 and MRP 14 form heterodimers and whether this complex may bind lipids of the cell membrane . ^^^ This finding opens new perspectives for the role of MRP 8 and MRP 14 in acute and chronic inflammatory processes . . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
During goitre surgery ( 25 patients ) and after major abdominal surgery ( 52 patients ) , we studied the plasma levels of endotoxin , interleukin 6 ( IL 6 ) , C reactive protein ( CRP ) , and the so called myeloid related proteins ( MRP ) , MRP 8 , MRP 14 , and the heterocomplex of both single proteins , MRP8 / MRP14 in three intervals : pre , intra , and postoperative . ^^^ Plasma levels were significantly elevated 2 and 6 h after minor surgery ( 3 , 619 micrograms / L ) , while no changes were observed in the plasma levels of MRP 8 and MRP 14 . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Expression of the two myeloic related proteins MRP 8 and MRP 14 is restricted to distinct stages of monocytic differentiation . ^^^ Monocytes were stimulated with pokeweed mitogen ( PWM ) in the presence or absence of interleukin 13 ( IL 13 ) , IL 4 and IL 10 , and secretion of MRP 8 , MRP 14 and MRP8 / 14 was assessed by using a sandwich enzyme linked immunosorbent assay system . ^^^ Peripheral monocytes secreted significantly increased amounts of MRP 14 and MRP8 / 14 but not MRP 8 under stimulation with PWM . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
In order to gain insight into the role of macrophages in human lung carcinomas , we investigated material from 35 lung carcinomas and 5 healthy lungs with 4 different antibodies ( CD 68 , MRP 8 , MRP 14 , 27E10 ) recognizing different macrophage subtypes . ^^^ Compared to healthy lungs , the infiltration of MRP 8 and MRP 14 positive macrophages was reduced in lung carcinomas while the number of 27E10 positive cells was enhanced . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
MRP 8 , MRP 14 and 27E10 were quantitatively evaluated in the intraalveolar , alveolar interstitial and alveolar intracapillary lung compartments . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
In cattle , the expression of markers for activated or recruited phagocytes , the calcium binding proteins S100A8 and S100A9 ( formerly called MRP 8 and MRP 14 , respectively ) , was largely restricted to cells showing weak or undetectable iNOS expression ; iNOS positive Mphi showed a low expression of S100A8 and S100A9 . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
The S 100 proteins MRP 8 and MRP 14 are expressed by cells of the myelomonocytic lineage , either alone or simultaneously during certain stages of cellular differentiation . ^^^ We demonstrate that MRP 14 but not MRP 8 was detected by immunostaining in the cytoplasm of epithelioid cells on the surface of round coverslips implanted for 14 days into the subcutaneous tissue of mice . ^^^ Purified or recombinant human MRP 14 but not MRP 8 inhibits the respiratory burst of BCG activated macrophages . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
SUMMARY : To assess the value of MRP 8 , MRP 14 , and MRP 8 / 14 serum concentrations as markers of disease progression in HIV infection and as markers of intercurrent infections . ^^^ DESIGN : We measured MRP 8 , MRP 14 , and MRP 8 / 14 serum concentrations in 184 HIV infected patients in various stages of disease with or without disease exacerbation and in 50 healthy control subjects . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Studies of our group show that the Ca ( 2+ ) binding proteins MRP 8 and MRP 14 as well as their heterocomplex MRP8 / 14 ( 27E10 epitope ) can be immunolocalized in the majority of granulocytes and macrophages in active but not inactive IBD . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Since it is suggested that macrophages play a central pathogenetic role in inflammatory neuropathies , we investigated the expression of macrophage differentiation antigens associated with acute ( MRP 14 and 27E10 antigens ) and more chronic inflammation ( MRP 8 and 25F9 antigens ) in 76 sural nerve biopsies from patients with Guillain Barr syndrome , chronic inflammatory demyelinating polyneuropathy , other inflammatory neuropathies , hereditary neuropathies and normal sural nerves . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
The 14 kDa myeloid related protein ( MRP 14 ) and its heterodimeric partner , MRP 8 , are members of the S 100 family of calcium binding proteins ( S100A9 and S100A8 , respectively ) . ^^^ MRP 8 has a regulatory role in MRP 14 activity , inhibiting the adhesion induced by MRP 14 through the formation of the heterodimer . ^^^ We postulate that MRP 14 and MRP 8 are important in vivo candidates for the regulated adhesion of neutrophils through control of Mac 1 activity . . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Electrospray ionization mass spectrometry indicated masses of 10 , 149 + / 2 Da for MRP 8 and 13 , 069 + / 2 Da for MRP 14 compared to masses calculated from proteins derived from their cDNA sequences of 10 , 211 and 13 , 214 Da , respectively , indicating posttranslational modifications and / or errors in the derived protein sequences . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
To further our understanding of MRP 8 and MRP 14 in the developmental stages of inflammatory responses , overexpression of the MRPs was obtained through a combination of a T 7 based expression vector and the Escherichia coli BL 21 ( DE 3 ) cell line . ^^^ Extensive biophysical characterization and chemical cross linking experiments show that MRP 8 and MRP 14 form oligomers with a strong preference to associate as a heterodimer . ^^^ Our results suggest that there is a unique complementarity in the interface of the MRP8 / MRP14 complex that can not be fully reproduced in the MRP 8 and MRP 14 homodimers . . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
The migration inhibitory factor related proteins ( MRPs ) MRP 8 and MRP 14 were detected in differentiated human leukemia cell lines ( THP 1 and HL 60 ) by immunocytochemical analysis . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Normal cells of the human esophageal epithelium expressed CAAF 1 , and also expressed the homologous novel S 100 proteins including CAAF 2 , MRP 8 , and MRP 14 , but not S100alpha . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Furthermore , the level of transcriptional expression of the regulatory calcium binding S 100 proteins MRP 8 , MRP 14 and CP 10 was associated with inflammatory demyelination and expression of IFN gamma , IL 2 , TNF alpha , and iNOS . . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Widespread expression of MRP 8 and MRP 14 in human cerebral malaria by microglial cells . ^^^ Here we report that microglial cells sensitive cellular sensors of threats to the central nervous system in CM express the myeloid related proteins MRP 8 ( S100A8 ) and MRP 14 ( S100A9 ) , Ca2+ binding sensor proteins of activated monocytes . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Using the two hybrid system we analyzed the dimerization of MRP 8 ( S100A8 ) and MRP 14 ( S100A9 ) , two S 100 proteins expressed in myeloid cells . ^^^ Interaction analysis of chimeric murine / human MRP 14 proteins indicates , that the C terminal EF hand domain plays a prominent role in MRP 8 MRP14 interaction and determines the specificity of dimerization . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Copurification of P 6 , MRP 8 , and MRP 14 from human granulocytes and separation of individual proteins . ^^^ A method is described for purification of P 6 , MRP 8 , and MRP 14 , three calcium binding proteins assigned to the S 100 protein family . ^^^ The purification procedure included preparation of human granulocytes , ammonium sulfate precipitation , and anion exchange chromatography and resulted in the copurification of P 6 , MRP 8 , and MRP 14 . ^^^ The procedure was carried out in the course of 4 days and yielded several milligrams of essentially pure P 6 , MRP 8 , and MRP 14 in either native or denatured form . . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
MRP 14 ( Macrophage migration inhibitory related protein ) and MRP 8 , two S 100 proteins contained in high concentrations in these cells are obviously essential for adhesion and migration of monocytes and granulocytes . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Myeloid related proteins 8 and 14 ( MRP 8 and MRP 14 ) are two Ca2+ binding proteins of the S 100 family highly abundant in myelomonocytic cells . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Studies in our institution have shown that the Ca ( 2+ ) binding proteins MRP 8 and MRP 14 as well as their heterocomplex MRP8 / 14 ( 27E10 epitope ) are expressed in the majority of granulocytes and macrophages in active but not inactive inflammatory bowel disease . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
The two migration inhibitory factor ( MIF ) related protein 8 ( MRP 8 ; S100A8 ) and MRP 14 ( S100A9 ) are two calcium binding proteins of the S 100 family . ^^^ Novel insights into structure and function of MRP 8 ( S100A8 ) and MRP 14 ( S100A9 ) . ^^^ Phagocytes expressing MRP 8 and MRP 14 belong to the early infiltrating cells and dominate acute inflammatory lesions . ^^^ In addition , elevated serum levels of MRP 8 and MRP 14 have been found in patients suffering from a number of inflammatory disorders including cystic fibrosis , rheumatoid arthritis , and chronic bronchitis , suggesting conceivable extracellular roles for these proteins . ^^^ In view of the current knowledge , the authors will hypothesize that MRP 8 and MRP 14 play an important role in leukocyte trafficking , but do not affect neutrophil effector functions . . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
In contrast , no epidermal expression of this biologically active heterodimer of the calcium binding proteins MRP 8 and MRP 14 was found in LIS . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Activation of macrophages / microglia with the calcium binding proteins MRP 14 and MRP 8 is related to the lesional activities in the spinal cord of HTLV 1 associated myelopathy . ^^^ The results indicate that a large number of macrophages and microglia express both MRP 14 and MRP 8 in active chronic inflammatory lesions of the patients with a short duration of illness ( 2 . 5 years ) . ^^^ In the patient whose duration of illness was 4 . 5 years , perivascular and parenchymal macrophages and microglia were reactive for MRP 8 but not for MRP 14 . ^^^ In contrast , MRP 14 and MRP 8 were negative on the perivascular and parenchymal macrophages and microglia in inactive chronic lesions and in controls . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
S100A12 is expressed exclusively by granulocytes and acts independently from MRP 8 and MRP 14 . ^^^ S100A12 , a recently described member of the S 100 protein family , has been shown to be coexpressed in granulocytes and monocytes together with two other S 100 proteins , MRP 8 ( S100A8 ) and MRP 14 ( S100A9 ) , and a functional relationship between these three S 100 proteins has been suggested . ^^^ S100A12 , MRP 8 , and MRP 14 translocate simultaneously from the cytosol to cytoskeletal and membrane structures in a calcium dependent manner . ^^^ However , no evidence for direct protein protein interactions of S100A12 with either MRP 8 or MRP 14 or the heterodimer was found by chemical cross linking , density gradient centrifugation , mass spectrometric measurements , or yeast two hybrid detection . ^^^ Thus , S100A12 acts individually during calcium dependent signaling , independent of MRP 8 , MRP 14 , and the heterodimer MRP8 / MRP14 . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
The expression of inducible nitric oxide synthase ( iNOS ) , major histocompatibility class 2 molecules ( MHC 2 ) , CD 68 , and the calcium binding proteins S100A8 and S100A9 ( also called MRP 8 and MRP 14 , respectively ) was assessed in lung tissues from cattle that succumbed to pneumonia . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
The regulatory role of MRP 8 ( S100A8 ) and MRP 14 ( S100A9 ) in the transendothelial migration of human leukocytes . ^^^ Recent investigations give evidence that the two myeloid related proteins MRP 8 ( S100A8 ) and MRP 14 ( S100A9 ) , which are abundant in activated or recruited phagocytes , may have a modulatory role in inflammatory responses . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
OBJECTIVE : To analyze which physiologic stimuli induce secretion of myeloid related protein 8 ( MRP 8 ) and MRP 14 , two S 100 proteins expressed in neutrophils and monocytes , and to determine whether serum concentrations of these proteins are reliable parameters for monitoring inflammatory activity in pauciarticular juvenile rheumatoid arthritis ( JRA ) . ^^^ METHODS : Secretion of MRP 8 and MRP 14 was analyzed using a coculture system of endothelial cells and monocytes . ^^^ The expression of MRP 8 and MRP 14 by leukocytes in synovial tissue or fluid was investigated using immunohistochemistry . ^^^ RESULTS : MRP 8 and MRP 14 were specifically released during interaction of activated monocytes with tumor necrosis factor stimulated endothelial cells . ^^^ In JRA patients , MRP 8 and MRP 14 were strongly expressed in infiltrating neutrophils and monocytes within the inflamed joints and could be found in significantly higher concentrations in synovial fluid ( mean 42 , 800 ng / ml ) compared with serum ( 2 , 060 ng / ml ) . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Calcium induced noncovalently linked tetramers of MRP 8 and MRP 14 detected by ultraviolet matrix assisted laser desorption / ionization mass spectrometry . ^^^ MRP 8 and MRP 14 are members of the S 100 family of calcium binding proteins which play an important role during calcium induced activation of phagocytes . ^^^ Furthermore , we could determine posttranslational modifications of MRP 8 and MRP 14 , the stoichiometric proportion of the two known MRP 14 isoforms in the complexes as well as the number of calcium ions bound to the single MRP 8 and MRP 14 monomers and tetramers . ^^^ MRP 14 showed a higher affinity for calcium than MRP 8 . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Recently , we identified the two myeloid related protein 8 ( MRP 8 ) ( S100A8 ) and MRP 14 ( S100A9 ) as fatty acid binding proteins ( Klempt , M . , Melkonyan , H . , Nacken , W . , Wiesmann , D . , Holtkemper , U . , and Sorg , C . ( 1997 ) FEBS Lett . 408 , 81 84 ) . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
We determined the pattern of expression of MRP 8 , MRP 14 , and the MRP8 / 14 heterodimer ( 27E10 antigen ) in rheumatoid arthritis ( RA ) synovial membrane ( SM ) . ^^^ METHODS : SM samples were obtained from patients with RA at joint replacement surgery or at arthroscopy and patients without joint disease ( healthy subjects ) and immunostained for MRP 8 , MRP 14 , 27E10 antigen ( MRP8 / 14 heterodimer ) , and CD 68 . ^^^ SM lining layer ( LL ) MRP 8 , MRP 14 , and 27E10 staining was observed predominantly adjacent to the CPJ and only in patients with active disease . ^^^ Synovial sublining layer ( SL ) MRP 8 , MRP 14 , and 27E10 staining was also observed only in patients with active disease , but in contrast to the LL , SL staining was observed predominantly in sections remote from the CPJ . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Human gingival crevicular fluid contains MRP 8 ( S100A8 ) and MRP 14 ( S100A9 ) , two calcium binding proteins of the S 100 family . ^^^ The presence of MRP 8 and MRP 14 in GCF was confirmed by Western blot , with monoclonal antibodies . ^^^ The two polypeptides , MRP 8 and MRP 14 , identified in GCF represent the major difference between the 2 D PAGE patterns of serum and GCF , and we hypothesize that they may play an important role in the gingival sulcus and could represent possible markers for periodontal diseases . . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Dynamics of microglial activation after human traumatic brain injury are revealed by delayed expression of macrophage related proteins MRP 8 and MRP 14 . ^^^ MRP 8 and MRP 14 , which are only expressed on activated macrophages and microglial cells , could be detected only within brains with a survival time of more than 72 h post TBI . ^^^ This delayed expression of the activation markers MRP 8 and MRP 14 and the proliferation marker MIB 1 is comparable to experimental closed head injuries but strictly different from acute activation found in ischemic brains . . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Calcium induced noncovalently linked tetramers of MRP 8 and MRP 14 are confirmed by electrospray ionization mass analysis . ^^^ Proteins of the S 100 family such as MRP 8 ( S100A8 ) and MRP 14 ( S100A9 ) and its isoform MRP14 * show two calcium binding sites ( EF hands ) per protein chain . ^^^ MRP 8 , MRP14 * , and MRP 14 , isolated from human granulocytes or monocytes , are known to form noncovalently associated complexes ; the exact stoichiometries of these complexes in the presence of calcium are still controversially discussed in the literature . ^^^ The present electrospray ionization mass spectrometry ( ESI MS ) study shows that MRP 8 , MRP14 * , and MRP 14 exist as heterodimers MRP8 / 14 * and MRP8 / 14 , respectively , in the absence of calcium confirming both a recent nuclear magnetic resonance study and a biochemical study on this topic . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
When tested alone , neither MRP 8 nor MRP 14 showed activity in the Candida growth assay . ^^^ However , equimolar concentrations of MRP 8 and MRP 14 demonstrated a potent growth inhibitory effect that was reversible by 30 microM zinc . ^^^ Truncated MRP 14 ( missing the C terminal GHHHKPGLGEGTP tail ) used in combination with MRP 8 demonstrated zinc reversible activity that was somewhat less than that with complete MRP 14 . ^^^ These results suggest that intact calprotectin , consisting of a heterodimer of MRP 8 and MRP 14 , is necessary to form a zinc binding site capable of inhibiting microbial growth . . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
In a subgroup of 10 patients , myeloid related proteins : MRP 8 , MRP 14 , and the soluble heterocomplex ( MRP8 / MRP14 ) levels were additionally studied . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
The brains showed no or a negligible vascular expression of MRP 8 and MRP 14 . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Upon interaction with activated endothelium , monocytes and neutrophils form complexes of myeloid related protein 8 ( MRP 8 ) ( S100A8 ) and MRP 14 ( S100A9 ) , two members of the calcium binding S 100 family that are secreted during transendothelial migration . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
The S 100 family proteins MRP 8 ( S100A8 ) and MRP 14 ( S100A9 ) form a heterodimer that is abundantly expressed in neutrophils , monocytes , and some secretory epithelia . ^^^ Furthermore MRP 8 / 14 and MRP 14 did not bind to a glycosaminoglycan minus cell line . ^^^ We therefore conclude that the MRP 8 / 14 complex binds to endothelial cells via the MRP 14 subunit interacting chiefly with heparan sulfate proteoglycans . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Among the genes that were down regulated in FLs were MRP 8 and MRP 14 , which are involved in adhesion . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
The myeloid related proteins MRP 8 ( S100A8 ) and MRP 14 ( S100A9 ) , two members of the S 100 family of calcium binding proteins , are co expressed and form a cell surface and cytoskeleton associated heterodimer upon calcium mobilization which is recognized by the mAb 27E10 . ^^^ The following observations imply a function of MRP8 / 14 in the transmigration process : ( 1 ) higher secretion of MRP8 / 14 from 27E10 ( + ) monocytes compared to 27E10 ( ) monocytes after interaction with activated endothelium , ( 2 ) higher expression of CD11b on 27E10 ( + ) compared to 27E10 ( ) monocytes , ( 3 ) up regulation of CD11b on 27E10 ( ) monocytes in the presence of MRP 14 or MRP8 / 14 heterodimers but not MRP 8 and ( 4 ) active participation of MRP 14 but not of MRP 8 in transmigration as shown by blocking with respective antibodies . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
METHODS : Macrophages were characterized by means of immunohistochemistry of the Ki M1P , 25F9 , MRP 8 , MRP 14 , and MRP8 / 14 antigens and by means of in situ hybridization of CSF 1 , its c fms coded corresponding receptor , and human monocytic serine esterase 1 ( HMSE 1 ) mRNA . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Calprotectin , a heterodimer of MRP 8 and MRP 14 with antimicrobial properties , is found in the cytosol of neutrophils , monocytes , and human gingival keratinocytes . ^^^ At each passage , cells were harvested and analyzed for quantitative expression of MRP 8 and MRP 14 subunit mRNA by RNase protection assays and calprotectin complex by enzyme linked immunosorbent assay . ^^^ Incubation with PMA stimulated increased expression of MRP 8 and MRP 14 mRNA within 2 h , peaking within 5 h . ^^^ MRP 8 and MRP 14 specific mRNA expression by immortalized keratinocytes appeared to be unaffected by LPS or IL 1 beta . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
The differential expression of 17 genes , including downregulation of MRP 8 and MRP 14 and upregulation of CYP1B1 , was observed in all four carcinoma lines compared to NHBE cells . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
We have now analyzed expression of LCA ( leucocyte common antigen ) , CD 68 ( phagocytic cells ) , HLA DR , DP , DQ ( MHC class 2 ) , MRP 8 ( myeloid related protein , S100A8 ) , MRP 14 ( S100A9 ) , LCF ( lymphocyte chemoattractant factor , IL 16 ) and NOS 2 ( inducible nitric oxide synthase ) in macrophages / microglial cells in 39 GBM relapses and their matched primary tumors . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
RESULTS : The following genes were more highly expressed in arthritis patients than in normal subjects : macrophage differentiation marker MNDA ( myeloid nuclear differentiation antigen ) , MRP 8 and MRP 14 ( migratory inhibitory factor related proteins ) ; signalling molecules JAK 3 ( janus kinase 3 ) and MAP kinase p 38 ( mitogen activated protein kinase ) ; receptors TNFR2 / p75 , C C chemokine receptor type 1 ( CCR 1 ) , C 10 C chemokine receptor type 4 ( CXCR 4 ) and integrin beta 1 ; and the cytokines / chemokines interleukin ( IL ) 1beta and IL 8 . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Implication of extracellular zinc exclusion by recombinant human calprotectin ( MRP 8 and MRP 14 ) from target cells in its apoptosis inducing activity . ^^^ This factor is composed of 8 and 14 kDa subunits , which have also been termed migration inhibitory factor related proteins MRP 8 and MRP 14 . ^^^ METHODS : The apoptosis inducing activity of recombinant human MRP 8 ( rhMRP 8 ) and recombinant human MRP 14 ( rhMRP 14 ) was examined against EL 4 lymphoma cells in vitro . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
The presence of an active phenotype of macrophages was studied by detection of myeloid related proteins 8 and 14 ( MRP 8 and MRP 14 , respectively ) . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
The time dependent expression of fibronectin , MRP 8 , MRP 14 and defensin in surgically treated human skin wounds . ^^^ Fibronectin , MRP 8 , MRP 14 and defensin were detected immunohistochemically in 46 surgically treated , fresh ( hours old ) human wounds and in wounds of 13 individuals who died immediately from fatal trauma ( airplane crashes or train rollovers ) . ^^^ In immediate fatal trauma , it was not possible to detect fibronectin outside of bleeding areas nor could inflammatory cells be visualized in the interstitium using MRP 8 , MRP 14 , or defensin antibodies . ^^^ Granulocytic infiltrates limited to the perivasal space could be detected 20 30 min after infliction of the wound expressing MRP 8 , MRP 14 and defensin . ^^^ It was also possible to detect fibronectin networks and MRP 8 , MRP 14 , and defensin positive granulocytes and macrophages in particular wounds up to 30 days old . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Myeloid related protein 14 ( MRP 14 ) and its heterodimeric partner , MRP 8 , are cytosolic calcium binding proteins , highly expressed in neutrophils and monocytes . ^^^ MRP 8 mRNA but not protein is present in the myeloid cells of these mice , suggesting that the stability of MRP 8 protein is dependent on MRP 14 expression . ^^^ A compensatory increase in other proteins was not detected in cells lacking MRP 8 and MRP 14 . ^^^ The data indicate that MRP 14 and MRP 8 are dispensable for many myeloid cell functions . . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Expression of calcium binding proteins MRP 8 and MRP 14 in inflammatory muscle diseases . ^^^ Expression of myeloid related proteins MRP 8 and MRP 14 , two calcium binding S 100 proteins , characterizes a proinflammatory subtype of macrophages . ^^^ We immunohistochemically investigated expression of MRP 8 and MRP 14 in muscle biopsies of 33 patients with dermatomyositis , polymyositis , and inclusion body myositis . ^^^ We found a clear association of expression of MRP 8 and MRP 14 by infiltrating macrophages with degeneration of myofibers . ^^^ Because MRP 8 and MRP 14 are secreted by activated macrophages we investigated if these proteins would have direct extracellular effects on myocytes . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Two S 100 proteins , namely S100A9 ( MRP 14 ) and S100A8 ( MRP 8 ) , constitute roughly 40 % of the cytosolic protein in these cells , implying by their pure abundance an important role in the effector functions of neutrophils . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Myeloid related proteins MRP 8 and MRP 14 of the S 100 family are EF hand calcium binding proteins . ^^^ MRP 8 and MRP 14 were co isolated from neutrophils on an anti p47phox matrix with oxidase cytosolic factors and identified by mass spectrometry . ^^^ MRP 8 and MRP 14 are absent from Epstein Barr virus immortalized B lymphocytes , and , coincidentally , these cells display weak oxidase activity compared with neutrophils . ^^^ This was confirmed ex vivo by co transfection of Epstein Barr virus transformed B lymphocytes with genes encoding MRP 8 and MRP 14 . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
OBJECTIVE : To analyze S 100 protein expression , in the form of myeloid related protein 8 ( MRP 8 ) , MRP 14 , and the heterodimer MRP8 / MRP14 , in psoriatic arthritis ( PsA ) patients compared with rheumatoid arthritis ( RA ) and spondylarthropathy ( SpA ) patients , and to determine the effect of methotrexate ( MTX ) on the MRP antigen expression in PsA patients . ^^^ Expression of MRP 8 , MRP 14 , and MRP8 / MRP14 in synovium was determined by immunohistochemistry . ^^^ RESULTS : MRP 8 , MRP 14 , and MRP8 / MRP14 levels were increased in serum , SF , and synovium from PsA , RA , and SpA patients . ^^^ In contrast , MRP 8 , MRP 14 , and MRP8 / MRP14 expression in the SLL of PsA patients is increased , particularly in perivascular regions , compared with that in RA and SpA patients , suggesting a central role of MRP proteins in transendothelial migration of leukocytes in PsA . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
OBJECTIVE : To analyze which cellular compartments are involved in the initial phase of systemic onset juvenile rheumatoid arthritis ( JRA ) , and to investigate the role that myeloid related protein 8 ( MRP 8 ) and MRP 14 , two S 100 proteins that are primarily expressed in phagocytes , play in the disease . ^^^ Concentrations of MRP 8 / MRP 14 in serum were determined by enzyme linked immunosorbent assay . ^^^ RESULTS : By analyzing biopsy samples from cutaneous rashes during the initial phase of systemic onset JRA , we discovered infiltration of leukocytes expressing MRP 8 and MRP 14 . ^^^ Serum concentrations of MRP 8 / MRP 14 were 120 fold higher compared with healthy controls and approximately 12 fold higher compared with patients with other inflammatory diseases . ^^^ Concentrations of MRP 8 / MRP 14 in patients with systemic onset JRA fell dramatically after remission was induced . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Proinflammatory cytokines upregulate expression of calprotectin ( L 1 protein , MRP 8 / MRP 14 ) in cultured human keratinocytes . ^^^ When expression of mRNA for the light ( MRP 8 ) and heavy ( MRP 14 ) calprotectin chain was determined by RT PCR , their respective levels were shown to be increased four to ninefold and three to fivefold after 24 h of combined stimulation with IFN gamma and TNF alpha . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Interleukin 10 influences the expression of MRP 8 and MRP 14 in human dendritic cells . ^^^ Two of them , the transcripts for the myeloid related proteins ( MRPs ) MRP 8 and MRP 14 , were examined more closely . ^^^ CONCLUSIONS : These data demonstrate that the genes for MRP 8 and MRP 14 are expressed in various DC subpopulations in different intensity , depending on their maturational status and are increased after the treatment with IL 10 . ^^^ Expression of the genes for MRP 8 and MRP 14 in DC has not been reported before . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Expression of MRP 8 and MRP 14 by macrophages is a marker for severe forms of glomerulonephritis . ^^^ We analyzed if the different forms of glomerulonephritis ( GN ) are associated with the appearance of certain phenotypes of infiltrating macrophages characterized by expression of MRP 8 and MRP 14 as well as their complex formation . ^^^ Immunohistochemical analysis of 89 renal biopsies with different forms of nephritis revealed that expression and complex formation of MRP 8 and MRP 14 by infiltrating macrophages in the glomeruli correlated with the severity of the inflammatory process . ^^^ In contrast , a high percentage of macrophages in the renal interstitium expressed MRP 8 and MRP 14 without concomitant formation of their complex , and they indicated a chronic type of inflammatory reaction in GN . ^^^ Immunosuppressive drugs had no direct effects on the expression of MRP 8 and MRP 14 in macrophages in vitro . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Concerning common gene modulation , MRP 8 and MRP 14 were highly upregulated in human skin epidermis after solar simulated radiation , vaseline application or tape stripping , and to a lower extent after sodium dodecyl sulfate . ^^^ Together , these results suggest that MRP 8 and MRP 14 may be general , yet highly sensitive , markers for a great variety of skin stresses and that they are implicated in several epidermal repair pathways . . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
The heterodimeric molecule formed by MRP 14 with its partner MRP 8 ( S100A8 ) is the major fatty acid carrier in neutrophils . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
We investigated cutaneous biopsies of the different forms of leprosy ( 41 untreated patients ) including leprosy reaction type 1 ( reversal reaction ) and type 2 ( erythema nodosum leprosum ) ( n = 18 ) for expression of MRP 8 and MRP 14 by subtypes of macrophages . ^^^ Concomitantly we determined serum levels of MRP 8 and MRP 14 by sandwich enzyme linked immunosorbent assay . ^^^ Expression of MRP 8 and MRP 14 by CD 68 positive macrophages was low in tuberculoid leprosy and rose significantly in borderline tuberculoid leprosy and especially in multibacillary forms , there being expressed by mycobacteria loaded foam cells . ^^^ A significant rise of MRP 8 and MRP 14 expression also occurred in lepra reactions compared to the corresponding non reactional forms . ^^^ In type 1 it was associated with expression of MRP 8 and MRP 14 by epitheloid and giant cells , which so far were considered not to express both proteins . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
AIM : To study the expression of myeloid related proteins ( MRP ) 8 and myeloid related proteins ( MRP ) 14 in human esophageal squamous cell carcinoma and to investigate if there was any correlation between MRP 8 and MRP 14 expression level and histopathological grade in these tumors . ^^^ METHODS : In this study , 65 cases of advanced esophageal squamous cell carcinoma were assessed for MRP 8 and MRP 14 expression using immunohistochemistry . ^^^ Statistical analysis was performed for the comparison of MRP 8 and MRP 14 expression in normal and tumor tissues , and their relationship with clinicopathological features . ^^^ RESULTS : Reduced or absent expression of MRP 8 and MRP 14 was observed in esophageal squamous cell carcinoma , with a significant difference between tumor tissues and normal tissues ( P < 0 . 01 and P < 0 . 01 for MRP 8 and MRP 14 , respectively ) . ^^^ However , no significant association was found between MRP 8 and MRP 14 expression and age or gender ( P > 0 . 05 ) . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
MRP 8 and MRP 14 control microtubule reorganization during transendothelial migration of phagocytes . ^^^ MRP 14 , in complex with its binding partner MRP 8 ( S100A8 ) , promotes polymerization of microtubules . ^^^ Phosphorylation of MRP 14 is antagonistically regulated by binding of MRP 8 and calcium . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Analysis of imiquimod treated warts demonstrated that imiquimod enhanced IL 6 expression and induced IL 8 , GM CSF , MRP 8 and MRP 14 . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Myeloid related protein ( MRP ) 8 / 14 ( calprotectin ) and its subunits MRP 8 and MRP 14 in plaque induced early gingival inflammation . ^^^ BACKGROUND : The inflammatory myeloid related protein , MRP8 / 14 , also called calprotectin , and its subunits MRP 8 and MRP 14 have been detected and identified recently in gingival crevicular fluid ( GCF ) . ^^^ Quantitative analyses of total proteins , MRP8 / 14 , MRP 14 and MRP 8 were performed by ELISA procedures . ^^^ Only minute amounts of the subunits MRP 8 and MRP 14 were detected in comparison with the complex MRP8 / 14 throughout the experiment . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Myeloid related protein 8 ( MRP 8 ) and MRP 14 , S 100 proteins secreted by activated phagocytes , bind specifically to endothelial cells . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
This result was confirmed by Northern blot analysis , which also showed that scalpel wounds expressed higher levels of MRP 8 , a related S 100 protein that can heterodimerize with MRP 14 , at days 2 , 7 , and 14 postwounding . ^^^ Free electron laser wounds also showed elevated expression of MRP 8 and MRP 14 relative to normal skin . ^^^ In situ hybridization showed that the patterns of MRP 14 and MRP 8 expression in free electron laser and scalpel wound tissues were similar . ^^^ MRP 14 and MRP 8 were expressed in the dermal wound margin , while a very low level of MRP 14 and MRP 8 expression was seen in the migrating epidermis . ^^^ Dual immunofluorescence staining for MRP 14 or MRP 8 and macrophage ( F4 / 80 ) showed that most of the wound macrophages simultaneously expressed MRP 14 and MRP 8 . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
To confirm these results and to examine the reproducibility and the applicability as a diagnostic marker , levels of the S100A8 ( MRP 8 ) / A9 ( MRP 14 ) heterocomplex in plasma and in synovial fluid were validated from patients with RA , OA , and other inflammatory joint diseases using enzyme immunoassay techniques . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
S100A9 ( MRP 14 ) and S100A8 ( MRP 8 ) are translocated into detergent resistant lipid structures only after calcium activation of the neutrophils . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Two S 100 family calcium binding proteins , macrophage inhibitory related factor protein 8 ( MRP 8 ) and MRP 14 , form a protein complex , MRP 8 / 14 , that inactivates CKII . ^^^ Analysis of MRP expression in primary keratinocytes and in HPV 16 and 18 transformed cervical and foreskin epithelial cell lines showed that expression of MRP 8 , MRP 14 , and the MRP 8 / 14 complex was detected only in primary untransformed keratinocytes and not in the HPV infected immortalized epithelial cells . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
BACKGROUND : To clarify the role of subclasses of macrophages in chronic glomerulonephritis , we evaluated the relationship between myeloid related protein 8 ( MRP 8 ) and MRP 14 expression on macrophages and the progression of membranoproliferative glomerulonephritis ( MPGN ) . ^^^ The first and second renal biopsy findings and MRP 8 and MRP 14 expression on macrophages were investigated in both groups . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
In addition , we found a general activation of the cutaneous epithelium reflected by the expression of the pro inflammatory S 100 proteins myeloid related protein 8 ( MRP 8 ) and MRP 14 . ^^^ It was accompanied by exclusive expression of MRP 8 , but not MRP 14 by the secretory cells of sweat glands . ^^^ Because MRP 8 and MRP 14 , released by epithelial cells , exhibit pro inflammatory effects on endothelial cells and leucocytes , the particular expression pattern of MRP 8 and MRP 14 in SOJIA is likely to represent a decisive early constitutive component in this inflammatory disease . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Macrophages were quantified by immunohistochemistry with antibodies to the PG M 1 , KP 1 , MRP 8 , MRP 14 and MRP8 / 14 antigens . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
The expressions of calprotectin mRNAs ( MRP 8 and MRP 14 ) were detected by Northern blotting . ^^^ P LPS increased MRP 14 mRNA expression in monocytes to the maximum level , about two times the control level after 24 hours treatment , but did not enhance the basal level of MRP 8 . ^^^ When the effects of TNF alpha and IL 1beta on those mRNAs were investigated , both MRP 8 and MRP 14 significantly increased to about 2 and 2 . 5 fold the control level , respectively . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Molecular basis of the complex formation between the two calcium binding proteins S100A8 ( MRP 8 ) and S100A9 ( MRP 14 ) . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Irrespective of granuloma type , a constant invasion of freshly recruited MRP 8 + and MRP 14 + macrophages was observed . ^^^ Tumor related sarcoid reactions were analyzed in 14 lymph nodes in comparison with sarcoidosis using immunohistochemical markers to lymphocytes ( CD 3 , CD 4 , CD 8 , and CD 20 ) , myeloid related protein ( MRP ) 8 and MRP 14 ( S100A8 and S100A9 ) , angiotensin 1 converting enzyme ( CD 143 ) , and mature or immature dendritic cells ( S 100 , HLA DR , fascin , CD 83 , and CD1a ) . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Genes abundantly expressed in all microglia cultures include CCL 2 ( MCP 1 ) , thymosin beta 10 , migration inhibitory factor related protein 8 ( MRP 8 ) , MRP 14 , corticotropin releasing factor receptor 1 and endothelin 2 . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
The subcellular distribution of myeloid related protein 8 ( MRP 8 ) and MRP 14 in human neutrophils . ^^^ BACKGROUND : Myeloid related protein 8 ( MRP 8 ) and MRP 14 are S 100 family calcium binding proteins that form a heterodimer known as calprotectin or MRP8 / 14 that is present in the cytosol of neutrophils and monocytes . ^^^ STUDY DESIGN : Three monoclonal antibodies specific for MRP 8 and MRP 14 were characterized and used in immunoblotting assays of neutrophil whole cell extracts , and isolated plasma membranes , primary granules , secondary granules and cytosol . ^^^ RESULTS : MRP 8 and MRP 14 were detected in neutrophil cytosol , plasma membrane , primary granule and secondary granule fractions . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Serum levels of myeloid related protein 8 ( MRP 8 ) / MRP 14 , matrix metalloproteinase 3 ( MMP 3 ) , and cartilage oligomeric matrix protein ( COMP ) were determined by enzyme linked immunosorbent assay . ^^^ The most prominent change in markers of inflammation was a reduction in the different macrophage subsets ( CD 68 , CD 163 , MRP 8 , and MRP 14 ) , but this was not paralleled by a decrease in serum MRP 8 / MRP 14 . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Comparison between responders ( n = 35 ) and nonresponders ( n = 17 ) showed differences in the degree of change in the levels of CD 163 in the lining and CD 163 , MMP 3 , and CD 3 in the sublining , whereas trends in change in the levels of MRP 8 and MRP 14 in the lining and sublining were similar in the 2 groups . ^^^ Class prediction analysis yielded a 90 % correct prediction using 8 synovial parameters , as follows : lining and sublining CD 163 , MRP 8 , and MRP 14 , sublining MMP 3 , and PMNs . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
On the other hand , the levels of a 10 . 1 kDa and a 13 . 0 kDa protein , identified as migration inhibitory factor related proteins ( MRP ) , MRP 8 and MRP 14 , respectively , began to increase from an early stage of acute rejection . ^^^ CONCLUSIONS : We identified three plasma proteins MRP 8 , MRP 14 , and lysozyme that increased during small bowel allograft rejection . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
The serum protein cystic fibrosis associated antigen ( CFAG ) , present at elevated levels in CF homozygotes and heterozygotes , is now known to consist of two distinct but related subunits ( calgranulins A ( CAGA ) and B ( CAGB ) ) . ^^^ We have previously assigned CAGA to human chromosome 1q12 q 21 and demonstrate here that the cDNA probe for CAGB cosegregates with it in our somatic cell hybrid panel . cDNA probes for the related genes calcyclin ( CACY ) and a mouse placental protein ( 18A2 , suggested name Capl ) enabled us to confirm and refine the in situ hybridization result assigning CACY to chromosome 1q21 25 and to demonstrate that both genes cosegregate with CAGA and CAGB . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
In the present study , we have analysed the functional promoter elements of the H . pylori cagA gene as well as of the divergently transcribed cagB gene . ^^^ Primer extension analyses identified a single 5 ' end of the cagA mRNA , while two initiation sites were mapped in the case of the cagB mRNA . ^^^ Deletion analyses indicated that the cagA and cagB genes are transcribed by overlapping promoters and that full activation requires sequences up to 70 and 96 respectively . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Most recently , several new members of the Ca2+ binding protein family have been discovered , e . g . , the cystic fibrosis antigen ( CFAg ) , the macrophage migration inhibitory factor related proteins ( MRP 8 and 14 ) , non muscle alpha actinin , and uvomorulin , the latter belonging to the group of cell adhesion molecules . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
The entire nucleotide sequence of the cDNA insert in the pEL 98 revealed a single open reading frame that encodes a 101 amino acid of putative protein ( hereafter referred to as the pEL 98 protein ) . pEL 98 protein is highly homologous to both alpha and beta subunits of bovine S 100 protein ( 49 and 47 % homology , respectively ) , a protein belonging to the family of calcium binding proteins . pEL 98 protein is also homologous to several S 100 related proteins such as `` calcyclin , ' ' a product of growth regulated human gene 2A9 , porcine p 11 ( or p 10 ) , the small subunit of a protein complex which serves as a major substrate of tyrosine kinase , and human cystic fibrosis antigen ( CFAg ) . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Monoclonal antibodies , specifically recognizing the tentatively named cystic fibrosis antigen ( CFAg ) , were produced . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
All the cell lines that were tested constitutively produce lymphokines , including colony stimulating factor ( CSF ) , erythroid potentiating activity ( EPA ) , macrophage migration inhibitory factory ( MIF ) , neutrophil inhibitory factor ( NIF ) , and differentiation inducing factor ( DIF ) . . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Indeed , partial sequence of other small lens proteins identifies another MIF related protein ( MRP 8 ) in calf lens . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
MAIN OUTCOME MEASURES : Detection of indicators of the inflammatory response : interleukin 1 ( IL 1 ) , interleukin 6 ( IL 6 ) , intercellular adhesion molecule ( ICAM 1 ) , antibacterial protein ( defensin 3 that reflects the activation of granulocytes ) , the antibody clone HAM 56 ( for detection of local macrophages ) , and antibodies against macrophage inhibiting factor ( MIF ) related proteins 8 and 14 ( MRP 8 and 14 ) . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
MAIN OUTCOME MEASURES : Correlation between presence of indicators of the inflammatory response : interleukin 1 ( IL 1 ) , interleukin 6 ( IL 6 ) , intercellular adhesion molecule 1 ( ICAM 1 ) , antibacterial protein ( defensin 3 reflecting the activation of granulocytes ) , the antibody clone HAM 56 ( for detection of local macrophages ) , and antibodies against macrophage migration inhibiting factor ( MIF ) related proteins 8 and 14 ( MRP 8 and 14 ) , and clinical state evaluated by the Mannheim Peritonitis Index ( MPI ) , the Peritonitis Index Altona 2 ( PIA 2 ) and the Acute Physiology Score ( APS ) . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
CONCLUSION : H pylori stimulates MIF release in monocytes , likely through its cag PAI , but not related to cagA or cagE . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Upon establishing a role for the H . pylori CagA virulence factor in MIF production , MIF binding to CD 74 on GEC was confirmed . rMIF and H . pylori were shown to increase GEC proliferation , which was decreased when cagA strains were used and when CD 74 was blocked by mAbs . ^^^ Apoptosis was also decreased by MIF , but increased by cagA strains that induced much lower amounts of MIF than the wild type bacteria . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
By immunoblotting experiments , Mac 387 was shown to react with a previously defined antigen called L 1 ; this is a multichain myelomoncytic protein of about 36 Kd which shows sequence homology with the cystic fibrosis antigen . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Up to now , a total of 40 cases of cutaneous macrophage disorders ( histiocytoses and granulomas ) and related diseases were examined using a panel of monoclonal and polyclonal antibodies to macrophage differentiation antigens ( mAb MS 1 , mAb alpha CD1a , mAb alpha CD 34 , mAb RM 3 / 1 , mAb alpha CD11c , mAb alpha CD 36 , mAb MAC 387 , mAb 27E10 , polyclonal antibodies alpha MRP 8 and 14 , mAb alpha CD 68 , mAb 25F9 , mAb DRC 1 R4 / 23 , and mAb 1F10 ) . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
By immunohistochemical evaluation , it was found that the crescents were composed principally of macrophages , as seen by CD 68 ( KP 1 ) , MRP 8 , MAC 387 , and HAM 56 expression . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
All genes are linked within 1 . 9 Mbp of human genomic DNA in the order : S100A10 , trichohyalin , profilaggrin , involucrin , SPRR 3 , SPRR1B , SPRR2A , loricrin , S100A9 , S100A9 , S100A8 , S100A6 . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
We found both genes within the cluster , with S100A11 being close to S100A10 and S100A12 between the genes S100A8 and S100A9 . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
The fatty acid binding heterocomplex FA p 34 formed by S100A8 and S100A9 is the major fatty acid carrier in neutrophils and translocates from the cytosol to the membrane upon stimulation . ^^^ The kFA p 34 complex , composed of S100A8 and S100A9 , has been implicated in the intracellular transport of arachidonic acid and its precursors in human keratinocytes . ^^^ Densitometrical analyses of 2D gels show that n and kFA p 34 contain twice as much S100A8 and S100A9 suggesting an estimated stoichiometry of ( S100A8 ) 2S100A9 . ^^^ A method is described allowing to distinguish n and kFA p 34 from S100A8 / S100A9 homo and heteromer complexes that are devoid of FA binding properties . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
The analysis of S100A9 and S100A8 expression in matched sets of macroscopically normal colon mucosa and colorectal carcinoma : the S100A9 and S100A8 positive cells underlie and invade tumor mass . ^^^ Furthermore , an additional protein , identified by matrix assisted laser desorption / ionization mass spectrometry ( MALDI MS ) as S100A8 , exhibited an increased expression in the same specimens of malignant tissues as the S100A9 protein . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
By contrast , other markers including the F4 / 80 antigen , the macrophage scavenger receptor , the S 100 proteins , S100A8 and S100A9 , and the secretory product lysozyme appeared later in development and appeared restricted to only a subset of c fms positive cells . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
In this paper we show that S100A8 mRNA is expressed without S100A9 mRNA between 6 . 5 and 8 . 5 days postcoitum within fetal cells infiltrating the deciduum in the vicinity of the ectoplacental cone . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
The lack of co expression of S100A9 with S100A8 in activated macrophages suggests distinct functions for the proteins expressed by different cell types . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Analysis of the calcium induced arachidonic acid ( AA ) binding to S100A8 / A9 revealed that maximal AA binding was achieved at molar ratios of 1 mol S100A8 and 1 mol S100A9 and for values greater than 3 calciums per EF hand . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Conversely , the expression of a novel variant of heat shock protein 70 and several members of the S 100 protein family of calcium binding proteins ( two isoforms of S100A9 , S100A8 , S100A11 and S100A6 ) were upregulated in transformed colon mucosa . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Recombinantly expressed murine S100A9 interacts in vitro with murine and human S100A8 in an in vitro glutathione S transferase pull down assay . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
S100A9 associates with A 8 in neutrophils , but was not coinduced with S100A8 . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
A . , we investigated , by immunohistochemistry , the presence of different S 100 proteins ( S100A1 , S100A2 , S100A3 , S100A4 , S100A5 , S100A6 , S100A8 , S100A9 , S100A12 and S100B ) in C . ^^^ Three S 100 proteins ( S100A8 , S100A9 , S100A12 ) which are highly expressed in activated macrophages and used as inflammatory markers were detected in C . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
S100A8 and S100A9 in inflammatory diseases . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
The heterodimeric complex of MRP 8 ( S100A8 ) and MRP 14 ( S100A9 ) . ^^^ The S 100 calcium binding proteins MRP 8 ( S100A8 ) and MRP 14 ( S100A9 ) form a heterodimeric complex in the cytosol of monocyte and neutrophil cell types circulating in peripheral blood . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Unexpectedly , the myeloid specific transcription factor C / EBPepsilon is not needed for the transcriptional upregulation of the S100A9 and S100A8 genes in neutrophils . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
An intact N terminus of annexin 1 and heteromeric assembly of S100A8 with S100A9 ( another member of the S 100 family ) appeared necessary for this interaction . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
The Ca2+ binding proteins S100A8 and S100A9 are encoded by novel injury regulated genes . ^^^ Here we show a strong up regulation of the RNA and protein levels of the two Ca ( 2+ ) binding proteins S100A8 and S100A9 in the hyperthickened epidermis of acute murine and human wounds and of human ulcers . ^^^ The increased expression of S100A8 and S100A9 in wound keratinocytes is most likely related to the activated state of the keratinocytes and not secondary to the inflammation of the skin , since we also found up regulation of S100A8 and S100A9 in the epidermis of activin overexpressing mice , which develop a hyperproliferative and abnormally differentiated epidermis in the absence of inflammation . ^^^ Furthermore , S100A8 and S100A9 expression was found to be associated with partially differentiated keratinocytes in vitro . ^^^ These results together with previously published data suggest that S100A8 and S100A9 are novel players in wound repair , where they might be involved in the reorganization of the keratin cytoskeleton in the wounded epidermis , in the chemoattraction of inflammatory cells , and / or in the defense against microorganisms . . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Selective expression of calcium binding proteins S100a8 and S100a9 at distinct sites of hair follicles . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
S100A8 , S100A9 and the S100A8 / A9 heterodimer complex specifically bind to human endothelial cells : identification and characterization of ligands for the myeloid related proteins S100A9 and S100A8 / A9 on human dermal microvascular endothelial cell line 1 cells . ^^^ We demonstrate that S100A8 , S100A9 and the S100A8 / A9 heterodimeric complex bind to human dermal microvascular endothelial cell line ( HMEC ) 1 with an increasing binding capacity progressing from S100A8 < or = S100A9 < or = S100A8 / A9 . ^^^ Similar results were obtained in the apolipoprotein E knockout mouse model , where preferably recombinant S100A9 but no S100A8 bound to the endothelium of the aorta ascendens . ^^^ The endothelial cell associated ligand proteins isolated by the use of the S100A9 monomer and the S100A8 / A9 dimer were subjected to mass spectrometry for protein identification . ^^^ Clearly , alpha ( 2 ) macroglobulin was identified as a binding partner for the S100A9 monomer , whereas no protein could be identified from the database for the ligand of the S100A8 / A9 dimer . . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Calgranulins S100A8 and S100A9 are negatively regulated by glucocorticoids in a c Fos dependent manner and overexpressed throughout skin carcinogenesis . ^^^ The two calgranulins S100A8 and S100A9 were found to be differentially expressed at sites of acute and chronic inflammation . ^^^ By comparing S100A8 and S100A9 mRNA levels in wild type and c Fos deficient mice ( c fos ( / ) ) we found that expression is negatively regulated by c Fos / AP 1 . ^^^ Glucocorticoids , which exhibit potent anti inflammatory and anti tumor promoting activities repressed TPA mediated S100A8 and S100A9 induction in wild type , but not in c fos ( / ) mice , thus identifying both genes as the first examples of AP 1 target genes whose repression of TPA induced transcription by glucocorticoids depends on c Fos . ^^^ These data identify S100A8 and S100A9 as novel , tumor associated genes and may point to an as yet unrecognized function of both genes in the development of epithelial skin tumors . . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
S100A8 , S100A9 , and S100A12 , collectively known as myeloid related proteins ( MRPs ) , are highly expressed by the myeloid cell lineage and are found in the extracellular milieu during infections and inflammatory conditions . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
This cluster contains 13 genes encoding S 100 calcium binding proteins , some of which ( S100A7 , S100A8 and S100A9 ) are known to be up regulated in individual patient keratinocytes . ^^^ These analyses demonstrated up regulation of S100A8 , S100A9 and , to a lesser extent , S100A7 and S100A12 , only in the 1q21 linked family . ^^^ We subsequently analysed S100A7 , S100A8 , S 100 A9 and S 100 A12 in three additional samples and were able to confirm S100A8 / S100A9 specific over expression in 1q linked pedigrees . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
By non radioactive in situ hybridization and immunohistochemistry on serial sections , it was demonstrated that IFNgamma+ T lymphocytes and S100A8+ , S100A9+ macrophages embrace granulomas , which consisted of S100A8 , S100A9 , HLA DR+ , CD40+ , TNFalpha+ macrophages . ^^^ During this process , they may be activated by IFNgamma T helper 1 lymphocytes and be differentiated to S100A8 , S100A9 sessile , HLA DR+ , CD40+ antigen presenting , TNFalpha+ pro inflammatory macrophages forming granulomas . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Interestingly , five calcium binding proteins ( S100A2 , S100A7 , S100A8 , S100A9 , and S100A10 ) were overexpressed . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
BACKGROUND : Calprotectin ( complex of S100A8 and S100A9 ) is the major calcium and zinc binding protein of phagocytes . ^^^ Analysis by electrophoresis and mass spectrometry showed that the patients ' protein contained normal S100A8 and S100A9 subunits . ^^^ INTERPRETATION : Dysregulation of zinc metabolism associated with accumulation in plasma of S100A8 and S100A9 defines a new disease , which encompasses a pathological role for dysregulation of two members of the large S 100 protein family . . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Together with two other calgranulins , S100A8 and S100A9 , it is mostly expressed in human granulocytes , although there is increasing evidence of expression in keratinocytes and psoriatic lesions . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
A novel inflammatory syndrome defined by extraordinarily high expression of S100A8 and S100A9 confirmed recent observations in vitro demonstrating a role of these proteins during recruitment of leukocytes . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Our present studies compare the level of expression , and cellular localization of S100A7 , S100A8 , S100A9 , S100A10 , and S100A11 in normal and psoriatic epidermis . ^^^ S100A8 and S100A9 are absent or are expressed at minimal levels in normal epidermis . ^^^ In involved psoriatic tissue , S100A10 and S100A11 levels remain unchanged , whereas , S100A7 , S100A8 , and S100A9 are markedly overexpressed . ^^^ S100A8 and S100A9 are strongly expressed in the basal and spinous layers in psoriasis involved tissue . ^^^ S100A8 and S100A9 did not form these larger complexes . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Together with S100A8 and S100A9 , it belongs to the calgranulin subfamily , i . e . it is mainly expressed in granulocytes , although there is an increasing body of evidence of expression in keratinocytes and psoriatic lesions . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Role of S100A8 and S100A9 in neutrophil recruitment in response to monosodium urate monohydrate crystals in the air pouch model of acute gouty arthritis . ^^^ OBJECTIVE : To examine the role of chemokines , S100A8 , and S100A9 in neutrophil accumulation induced by the causative agent of gout , monosodium urate monohydrate ( MSU ) crystals . ^^^ Release of chemokines , S100A8 , S100A9 , and S100A8 / A9 in response to MSU crystals was quantified by enzyme linked immunosorbent assays . ^^^ S100A8 , S100A9 , and S100A8 / A9 were also found at high levels in the pouch exudates following injection of MSU crystals . ^^^ In addition , injection of S100A8 , S100A9 , or S100A8 / A9 led to the accumulation of neutrophils in the murine air pouch , demonstrating their proinflammatory activities in vivo . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
S100A8 and S100A9 calcium binding proteins : localization within normal and cyclosporin A induced overgrowth gingiva . ^^^ S100A8 and S100A9 , also called myeloid related protein ( MRP ) 8 and 14 , are calcium binding proteins highly expressed in neutrophils , in which they play a key role in the inflammatory progression . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
S100A8 , the heterodimerization partner of S100A9 was not detectable in peripheral blood cells , suggesting that even a deficiency in both S100A8 and S100A9 proteins was compatible with viable and mature neutrophils . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Evidence for the involvement of the unique C tail of S100A9 in the binding of arachidonic acid to the heterocomplex S100A8 / A9 . ^^^ Protein complexes formed by S100A8 and S100A9 represent the only AA binding capacity in the human neutrophilic cytosol and are involved in the intracellular arachidonic acid metabolism . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
In addition , evaluation of chemokine and cytokine mRNA and protein expression revealed that sHZ induces the expression of chemokines , macrophage inflammatory protein ( MIP ) 1alpha / CCL3 , MIP 1beta / CCL4 , MIP 2 / CXCL2 , and monocyte chemoattractant protein 1 / CCL2 ; chemokine receptors , CCR 1 , CCR 2 , CCR 5 , CXCR 2 , and CXCR 4 ; cytokines , IL 1beta and IL 6 ; and myeloid related proteins , S100A8 , S100A9 , and S100A8 / A9 , in the air pouch exudates . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
S100A8 and S100A9 may play different roles in early embryonic development . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
The neutrophil cytoplasmic protein S100A8 / A9 ( along with S100A8 and S100A9 ) is chemotactic and stimulates neutrophil adhesion by activating the beta 2 integrin CD11b / CD18 . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
The three members of this group , S100A8 , S100A9 and S100A12 are overexpressed at local sites of inflammation . ^^^ Both the S100A8 / S100A9 complex and S100A12 have been proven to be useful as diagnostic markers of inflammation especially in non infectious inflammatory diseases such as arthritis , chronic inflammatory lung and bowel disease . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Fourteen S 100 protein genes are located within the epidermal differentiation complex on human chromosome 1q21 and 13 S 100 proteins ( S100A2 , S100A3 , S100A4 , S100A6 , S100A7 , S100A8 , S100A9 , S100A10 , S100A11 , S100A12 , S100A15 , S100B , and S100P ) are expressed in normal and / or diseased epidermis . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Chemotactic activity of S100A8 and S100A9 . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Proinflammatory activities of S 100 : proteins S100A8 , S100A9 , and S100A8 / A9 induce neutrophil chemotaxis and adhesion . ^^^ S100A8 and S100A9 are small calcium binding proteins that are highly expressed in neutrophil and monocyte cytosol and are found at high levels in the extracellular milieu during inflammatory conditions . ^^^ In this study , we report that S100A8 , S100A9 , and S100A8 / A9 caused neutrophil chemotaxis at concentrations of 10 ( 12 ) 10 ( 9 ) M . ^^^ S100A8 , S100A9 , and S100A8 / A9 stimulated shedding of L selectin , up regulated and activated Mac 1 , and induced neutrophil adhesion to fibrinogen in vitro . ^^^ However , neutrophil activation by S100A8 , S100A9 , and S100A8 / A9 did not induce actin polymerization . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
It was revealed that genes involved in squamous cell differentiation were coordinately downregulated , including annexin 1 , small proline rich proteins ( SPRRs ) , calcium binding S 100 proteins ( S100A8 , S100A9 ) , transglutaminase ( TGM 3 ) , cytokeratins ( KRT 4 , KRT 13 ) , gut enriched Krupple like factor ( GKLF ) and cystatin A . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
In vitro analysis of oxidase activation as well as protein protein interaction studies revealed that S100A8 is the privileged interaction partner for the NADPH oxidase complex since it bound to p67phox and Rac , whereas S100A9 did interact with neither p67phox nor p47phox . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Interestingly , expression of numerous S 100 family members was observed , and eight members , including S100A8 and S100A9 , were among the most differentially expressed genes . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Human S100A12 is localized in the S 100 gene cluster between S100A8 and S100A9 , which are neighbors in both mouse and human . ^^^ Confirming identical gene order , we found a DNA region between the murine S100A8 and S100A9 genes that is 60 . 9 % identical to a region of the human S100A12 gene , including the first exon . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
However , in marked contrast to other vascular beds , neutralizing antibodies to S100A8 had only a weak anti neutrophil recruitment effect and antibodies against the related S100A9 were ineffective . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Semiquantitative reverse transcriptase PCR analysis in 10 carious and 10 healthy pulpal tissue samples of the S 100 family members S100A8 , S100A9 , S100A10 , S100A12 , and S100A13 ; the cytokines tumor necrosis factor alpha ( TNF alpha ) , interleukin 1beta ( IL 1beta ) , IL 8 , IL 6 , and epithelial cell derived neutrophil attractant 78 ( ENA 78 ) ; and the structural protein collagen 1alpha indicated that all genes tested , with the exception of S100A10 , were more abundantly expressed in carious teeth . ^^^ Immunohistochemical studies in carious pulpal tissue indicated that S100A8 and the S100A8 / S100A9 complex were predominantly expressed by infiltrating neutrophils . ^^^ Gene expression analyses in immune system cells supported these findings and indicated that bacterial activation of neutrophils caused upregulation of S100A8 , S100A9 , and S100A13 . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
In this study the expression of S100A8 / S100A9 and S100A12 , pro inflammatory proteins specific for early recruited phagocytes , was studied in biopsies from 36 patients with giant cell arteritis . ^^^ The S100A8 / S100A9 complex was found to be abundant in the adventitia and media in affected arteries . ^^^ Both S100A8 / S100A9 and S100A12 serum concentrations were significantly higher in patients with giant cell arteritis than in healthy controls . ^^^ These data indicate that phagocytes within the adventitia and media contribute to the process of inflammation via release of the pro inflammatory S 100 proteins S100A8 , S100A9 , and S100A12 . . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Expression of S 100 proteins in normal human tissues and common cancers using tissue microarrays : S100A6 , S100A8 , S100A9 and S100A11 are all overexpressed in common cancers . ^^^ S100A6 , S100A8 , S100A9 and S100A11 have all been suggested to have potential roles in carcinogenesis and tumour progression but their expression has not been described in a wide range of human tissues and tumours . ^^^ METHODS AND RESULTS : A custom made tissue array , containing 291 tissue cores representing 28 tissue types and 21 tumour types , was used to produce sections that were immunostained for S100A2 , S100A6 , S100A8 , S100A9 , S100A11 , calbindin 1 , calbindin 2 , S100B and parvalbumin . ^^^ S100A6 , S100A8 and S100A9 were expressed in 32 % , 12 % and 28 % of breast cancers , respectively . ^^^ CONCLUSIONS : S100A6 , S100A8 , S100A9 and S100A11 are all expressed in common cancers , especially breast cancer . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Using a cDNA array screening approach , real time RT PCR , and Western blot analysis , we demonstrate that IL 22 up regulates , in a dose dependent manner , the expression of S100A7 , S100A8 , S100A9 , a group of proinflammatory molecules belonging to the S 100 family of calcium binding proteins , as well as the matrix metalloproteinase 3 , the platelet derived growth factor A , and the CXCL 5 chemokine . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Polymorphonuclear neutrophils from S100A9 ko mice are also devoid of S100A8 . ^^^ Similar , the calcium signals were not distinguishable from S100A9 deficient and wildtype neutrophils upon stimulation with platelet activating factor ( PAF ) , thapsigargin or macrophage inflammatory protein 1 alpha ( MIP 1 alpha ) , indicating despite their massive expression S100A8 / A9 do neither serve as calcium nor as zinc buffering proteins in granulocytes . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Elevated serum levels of myeloid related protein 8 ( also called S100A8 ) and myeloid related protein 14 ( S100A9 ) in children with juvenile idiopathic arthritis can indicate clinically occult disease activity . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
CONCLUSION : Of the upregulated S 100 genes , calgranulin members of S 100 genes ( S100A8 , S100A9 , and S100A12 ) were most highly expressed in the acute phase . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
S100A8 and S100A9 are members of the S 100 family of Ca2+ binding proteins and are now accepted as markers of inflammation . ^^^ In this study , regulation and expression of S100A8 and S100A9 were examined in fibroblasts . ^^^ Induction of S100A8 in the absence of S100A9 was confirmed in primary fibroblasts . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
S100A8 and S100A9 in human arterial wall . ^^^ S100A8 and S100A9 , the Ca2+ binding neutrophil cytosolic proteins , are associated with innate immunity and regulate processes leading to leukocyte adhesion and transmigration . ^^^ In neutrophils and monocytes the S100A8 S100A9 complex regulates phosphorylation , NADPH oxidase activity , and fatty acid transport . ^^^ S100A9 but not S100A8 was found in macrophages in ApoE / murine atherosclerotic lesions , whereas both proteins are expressed in human giant cell arteritis . ^^^ Here we demonstrate S100A8 and S100A9 protein and mRNA in macrophages , foam cells , and neovessels in human atheroma . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Calcium binding proteins S100A8 and S100A9 as novel diagnostic markers in human prostate cancer . ^^^ We examined the expression of two members of this family , S100A8 and S100A9 , together with the S 100 receptor RAGE ( receptor for advanced glycation end products ) in human prostate adenocarcinomas and in prostatic intraepithelial neoplasia . ^^^ EXPERIMENTAL DESIGN : Tissue specimens of 75 patients with organ confined prostate cancer of different grades were analyzed by immunohistochemistry for expression of S100A8 , S100A9 , and RAGE . ^^^ In addition , in situ hybridization of S100A8 and S100A9 was done for 20 cases . ^^^ RESULTS : S100A8 , S100A9 , and RAGE were up regulated in prostatic intraepithelial neoplasia and preferentially in high grade adenocarcinomas , whereas benign tissue was negative or showed weak expression of the proteins . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
We also examined the expression of other S 100 proteins , including S100A8 , S100A9 and S100A12 , and found that IPA preferentially up regulated S100P at the early stages of differentiation . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Prior to the disease onset , two chemotactic proteins ( S100A8 and S100A9 ) previously mapped to the psoriasis susceptibility region PSORS 4 , are strongly induced in mutant keratinocytes in vivo and in vitro . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Quantitative Northern blot analysis detected no S100A3 , S100A8 , S100A9 or S100A14 mRNA in mouse brain suggesting that these family members are not expressed in the brain . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
We previously identified a carboxylate modification of N linked glycans that is recognized by S100A8 , S100A9 , and S100A12 . ^^^ This was accompanied by reduction in inflammatory cells , reduced expression of proinflammatory cytokines and of S100A8 , S100A9 , and receptor for advanced glycation end products . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Expression of surfactant protein C , S100A8 , S100A9 , and B cell markers in renal allografts : investigation of the prognostic value . ^^^ Microarray analysis and real time PCR validation showed that surfactant protein C ( SP C ) , S 100 calcium binding protein A 8 ( S100A8 ) , S100A9 , and beta globin levels distinguished the two groups . ^^^ The prognostic value of mRNA transcripts was tested in an independent cohort of 43 rejection biopsies . mRNA and protein expression of S100A8 and S100A9 in infiltrating cells was significantly higher in the NP group compared with the PR group . ^^^ Relatively high expression of S100A8 and S100A9 during acute rejection is associated with a favorable prognosis , and high SP C expression is associated with an unfavorable prognosis . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
S100A8 , S100A9 and S100A12 comprise a new group of inflammation associated proteins that are constitutively expressed by neutrophils and inducible in numerous inflammatory cells . ^^^ We investigated expression of S100B , S100A8 , S100A9 and S100A12 in brain samples from sporadic and familial ( PS 1 ) AD cases and controls using immunohistochemistry and Western blot analysis . ^^^ S100B , S100A9 and S100A12 , but not S100A8 , were consistently associated with the neuropathological hallmarks of AD . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
S100P and S100Z were both up regulated in cancer ; whereas S100A7 , S100A8 and S100A9 were strongly up regulated only in DCIS . ^^^ One of them was characterized by the high concomitant expression of S100A7 , S100A8 and S100A9 . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Here , we identify S100A7 ( psoriasin ) , S100A8 , and S100A9 , members of the S100A family of calcium binding proteins , as novel BRCA 1 repressed targets . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
S100A8 and S100A9 activate MAP kinase and NF kappaB signaling pathways and trigger translocation of RAGE in human prostate cancer cells . ^^^ Deregulated expression of S 100 proteins , including S100A8 and S100A9 , was reported in association with neoplastic disorders . ^^^ In a previous study , we identified enhanced expression of S100A8 and S100A9 in human prostate cancer . ^^^ To investigate potential functional implications of S100A8 and S100A9 in prostate cancer , we examined the influence of over expressed and of purified recombinant S100A8 and S100A9 proteins in different prostate epithelial cell lines . ^^^ S100A8 and S100A9 were secreted by prostate cancer cells , a finding which prompted us to analyze a possible function as extracellular ligands . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
As recently S 100 proteins have been identified as ligands of RAGE , we sought to determine the effects of the proinflammatory heterodimer of S100A8 / S100A9 on the RAGE NFkappaB mediated induction of proinflammatory gene expression . ^^^ Following this preactivation , cells were stimulated for 48 h with heterodimeric human recombinant S100A8 / S100A9 . ^^^ RESULTS : Heterodimeric S100A8 / S100A9 enhanced secretion of IL 6 , ICAM 1 , VCAM 1 and MCP 1 in AGE albumin pretreated HUVEC in a dose dependent manner . ^^^ These effects could not be detected after stimulation with the homodimeric proteins S100A8 , S100A9 , S100A1 and S100B . ^^^ The effects of heterodimeric S100A8 / S100A9 were reduced by inhibition of the MAP kinase pathways ERK1 / 2 and p 38 by PD 98059 and SB 203580 , respectively . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
S100A8 and S100A9 , two Ca2+ binding proteins of the S 100 family , are secreted as a heterodimeric complex ( S100A8 / A9 ) from neutrophils and monocytes / macrophages . ^^^ Serum and synovial fluid levels of S100A8 , S100A9 , and S100A8 / A9 were all higher in patients with rheumatoid arthritis ( RA ) than in patients with osteoarthritis ( OA ) , with the S100A8 / A9 heterodimer being prevalent . ^^^ S100A8 / A9 complexes , as well as S100A9 homodimers , stimulated the production of proinflammatory cytokines , such as tumor necrosis factor alpha , by purified monocytes and in vitro differentiated macrophages . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
METHODS : The lungs of 20 day old congenic C57BL / 6 ( before any overt signs of inflammation ) and BALB / c CF mouse lines maintained in sterile environments were investigated for distinctions in the neutrophil chemokines S100A8 and S100A9 by quantitative RT PCR and RNA in situ hybridization , that were then correlated to neutrophil numbers . ^^^ RESULTS : The lungs of C57BL / 6 CF mice had spontaneous and significant elevation of both neutrophil chemokines S100A8 and S100A9 and a corresponding increase in neutrophils , in the absence of detectable pathogens . ^^^ In contrast , BALB / c CF mouse lungs maintained under identical conditions , had similar elevations of S100A9 expression and resident neutrophil numbers , but diverged in having normal levels of S100A8 . ^^^ CONCLUSION : The results indicate early and spontaneous lung inflammation in CF mice , whose progression corresponds to increased expression of both S100A8 and S100A9 , but not S100A9 alone . ^^^ Moreover , since both C57BL / 6 and BALB / c CF lungs were maintained under identical conditions and had similar elevations in S100A9 and neutrophils , the higher S100A8 expression in the former ( or suppression in latter ) is a result of secondary genetic influences rather than environment or differential infection . . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
BACKGROUND AND OBJECTIVE : Calprotectin is composed of two proteins , S100A8 and S100A9 , which are S 100 family members , and is detected in gingival crevicular fluid and gingival tissue with inflammation . ^^^ The expression of S100A8 / S100A9 mRNAs was examined by northern blotting and the amount of calprotectin was measured by enzyme linked immunosorbent assay ( ELISA ) . ^^^ RESULTS : Norepinephrine stimulated the expression of S100A8 / S100A9 mRNAs via beta adrenergic receptors in U 937 cells and significantly increased calprotectin production to about 3 . 6 fold that of the control . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Calcium dependent tetramer formation of S100A8 and S100A9 is essential for biological activity . ^^^ The heterodimers of S100A8 and S100A9 represent the major calcium binding proteins in phagocytes . ^^^ Calcium binding induces formation of ( S100A8 / S100A9 ) 2 tetramers . ^^^ Mutations in EF hand 2 did not interfere with formation of the S100A8 / S100A9 heterodimer as demonstrated by yeast two hybrid experiments and pull down assays . ^^^ In contrast , mass spectrometric analysis and density gradient centrifugation revealed that calcium induced association of ( S100A8 / S100A9 ) 2 tetramers was strictly dependent on a functional EF hand 2 in S100A9 . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
OBJECTIVES : We studied whether serum levels of S 100 proteins A 8 ( S100A8 ) and A 9 ( S100A9 ) are elevated in psoriasis , correlated their amounts with disease activity and identified potential cellular sources . ^^^ METHODS : Serum obtained from psoriasis patients or from healthy individuals was studied for S100A8 and S100A9 levels by enzyme linked immunosorbent assay . ^^^ Cellular sources of S100A8 and S100A9 were identified by in situ hybridization and immunohistochemistry of lesional psoriatic and nonlesional , nonpsoriatic skin . ^^^ RESULTS : A significant increase of S100A8 / S100A9 serum levels was found in patients with psoriasis compared with healthy controls . ^^^ Performing in situ hybridization of lesional psoriatic skin we detected a dramatic induction of both S100A8 and S100A9 mRNA and protein primarily in the suprabasal layers of the epidermis while expression was negligible in nonlesional , nonpsoriatic interfollicular epidermis . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
Transcription factors E2F5 and early growth response 1 ( EGR 1 ) , Ca ( 2+ ) signaling molecules S100A8 and S100A9 , and two oxidative stress induced genes were identified as immediate early genes of KC . ^^^
Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
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Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
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Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
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Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
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Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
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Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
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Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
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Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
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Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
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Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
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Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
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Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
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Interacting proteins: P06702 and P05109	Pubmed	SVM Score :0.0
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