| Interacting proteins: P06493 and P68400 |
Pubmed |
SVM Score :0.0 |
| Data from these in vitro phosphorylation studies suggest that AK is most likely not an efficient substrate for PKA , PKG , CaMKII , CK 1 , CK 2 , MAPK , Cdk 1 , or Cdk 5 . ^^^ |
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| Interacting proteins: P06493 and P68400 |
Pubmed |
SVM Score :0.0 |
| In all the species investigated , the protein contains several consensus phosphorylation sites for cyclin dependent kinases and CK 2 , and we have shown that the fish protein ( like mammalian NUCKS ) indeed is a substrate for CDK 1 and CK 2 in vitro . ^^^ |
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| Interacting proteins: P06493 and P68400 |
Pubmed |
SVM Score :0.0 |
| In this work , by in vitro kinase reactions and mass spectrometry analysis of the products , we have mapped phosphorylation sites in the N terminus of Mcm 2 by Cdc 7 , Cdk 2 , Cdk 1 , and CK 2 . ^^^ Three serine / proline sites were identified for Cdk 2 and Cdk 1 , and a unique site was phosphorylated by CK 2 . ^^^ |
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| Interacting proteins: P06493 and P68400 |
Pubmed |
SVM Score :0.0 |
| Two series of synthetic peptides that reproduce the amino and carboxyl terminal segments of the beta subunit of casein kinase 2 , including the sites phosphorylated by CK 2 and cdc 2 kinase , respectively , have been used as model substrates for these enzymes . ^^^ The N terminal peptide beta ( 1 9 ) , MSSSEEVSW , is readily phosphorylated by CK 2 but not all by cdc 2 . ^^^ The opposite is true of the C terminal peptide beta ( 206 215 ) , NFKSPVKTIR , whose Ser 4 is a good target for cdc 2 while being unaffected by CK 2 . ^^^ These observations would rule out the possibility that the site specificity of CK 2 might overlap with that of cdc 2 and possibly of other Pro directed protein kinases . . ^^^ |
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| Interacting proteins: P06493 and P68400 |
Pubmed |
SVM Score :0.0 |
| We propose that successive Cdc 2 and CKII phosphorylation could modulate nucleolin function in controlling cell cycle dependent nucleolar function and organization . ^^^ |
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| Interacting proteins: P06493 and P68400 |
Pubmed |
SVM Score :0.0 |
| In addition , the cdc 2 protein kinase , which is central to cell division cycle control , serves as a substrate for CKII specifically during the G 1 phase of human cells . ^^^ |
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| Interacting proteins: P06493 and P68400 |
Pubmed |
SVM Score :0.0 |
| Activities of PKC , PKA , cdc 2 kinase and CK 2 in ML 1 cells were changed early by bufalin ; PKA and PKC activities were inhibited , and cdc 2 kinase and CK 2 activities were increased . ^^^ |
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| Interacting proteins: P06493 and P68400 |
Pubmed |
SVM Score :0.0 |
| Protein kinase CK 1 can not replace CK 2 as phosphorylating agent and cdc 2 only slowly phosphorylates Rev at one of the two sites affected by MAP kinase . ^^^ |
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| Interacting proteins: P06493 and P68400 |
Pubmed |
SVM Score :0.0 |
| The comparison of GSK 3 with other kinases suggests some similar features of this kinase with proline directed protein kinases , such as cdc 2 or mitogen activated protein kinase ( MAP Kinases , ) and also with casein kinase 2 ( CK 2 ) . ^^^ |
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| Interacting proteins: P06493 and P68400 |
Pubmed |
SVM Score :0.0 |
| The nucleolar protein gar 2 of fission yeast is structurally related to the multifunctional nucleolar protein nucleolin from vertebrates and has been shown to be implicated in production of 18S rRNA . gar 2 contains several potential casein kinase 2 ( CK 2 ) phosphorylation sites and a single putative p 34 ( cdc 2 ) phosphorylation site in the consensus S50PKK . ^^^ Here , we show that , like nucleolin , gar 2 is phosphorylated in vitro by both highly purified CK 2 from CHO cells and p 34 ( cdc 2 ) from starfish oocytes . ^^^ |
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| Interacting proteins: P06493 and P68400 |
Pubmed |
SVM Score :0.0 |
| Nuclear localization sequence ( NLS ) dependent nuclear import of SV 40 large tumor antigen ( T Ag ) fusion proteins is regulated by phosphorylation sites for casein kinase 2 ( CKII ) and the cyclin dependent kinase Cdc 2 amino terminal to the NLS ( amino acids 126 132 ) . ^^^ Between the T Ag CKII and Cdc 2 sites is a site ( Ser 120 ) for the double stranded DNA dependent protein kinase ( dsDNA PK ) , which we show here for the first time to play a role in regulating T Ag nuclear import . ^^^ |
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| Interacting proteins: P06493 and P68400 |
Pubmed |
SVM Score :0.0 |
| In addition , either an acidic domain of nucleolin without phosphorylation sites , or an acidic domain containing 4 CK 2 sites , or a cluster of 5 cdc 2 sites was fused to the MBP nuclear localization signal ( MBP NLS ) . ^^^ Nuclear targetting of MBP by the bipartite nuclear localization signal of nucleolin became significantly more efficient after addition of either CK 2 sites or cdc 2 sites to the MBP NLS construct . ^^^ |
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| Interacting proteins: P06493 and P68400 |
Pubmed |
SVM Score :0.0 |
| Whereas mutants changing the cdc 2 phosphorylation site at position 315 of human p 53 had only little effect on Tms1 / p53 complex formation , we found that mutants involving the protein kinase CK 2 site at position 392 showed a significantly decreased relative affinity for the Tms 1 protein . ^^^ |
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| Interacting proteins: P06493 and P68400 |
Pubmed |
SVM Score :0.0 |
| A significant decrease in the activity of CKII and in the protein amount of p 53 , wafl / p21 , and Cdc 2 p34 was found . ^^^ |
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| Interacting proteins: P06493 and P68400 |
Pubmed |
SVM Score :0.0 |
| Its phosphorylation by cdc 2 , CK 2 , and PKC zeta modulate some of its activities . ^^^ |
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| Interacting proteins: P06493 and P68400 |
Pubmed |
SVM Score :0.0 |
| More subtle mutations within the C terminus of the protein , including alterations of the cdc 2 and CKII phosphorylation sites , had no effect on the transcriptional trans activation , mdm 2 or E 6 binding functions , indicating that phosphorylation of these sites is not essential for these activities . ^^^ |
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| Interacting proteins: P06493 and P68400 |
Pubmed |
SVM Score :0.0 |
| We have shown previously that the protein kinase CKII catalyses the phosphorylation of Ser 39 in Cdc 2 during the G ( 1 ) phase of the HeLa cell division cycle [ Russo , Vandenberg , Yu , Bae , Franza and Marshak ( 1992 ) J . ^^^ The S . cerevisiae homologue of Cdc 2 , Cdc 28 , contains a consensus CKII site ( Ser 46 ) , which is homologous with that of human Cdc 2 . ^^^ |
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| Interacting proteins: P06493 and P68400 |
Pubmed |
SVM Score :0.0 |
| Casein kinase 2 ( CKII ) phosphorylates mSTI 1 at S 189 , and cdc 2 kinase ( p34cdc2 ) at T 198 , substantiating a predicted CKII p34cdc2 NLS ( CcN ) motif . ^^^ |
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| Interacting proteins: P06493 and P68400 |
Pubmed |
SVM Score :0.0 |
| A pea homologue of human DNA helicase 1 is localized within the dense fibrillar component of the nucleolus and stimulated by phosphorylation with CK 2 and cdc 2 protein kinases . ^^^ The DNA and RNA unwinding activities were upregulated after phosphorylation of PDH 65 with CK 2 and cdc 2 protein kinases . ^^^ |
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| Interacting proteins: P06493 and P68400 |
Pubmed |
SVM Score :0.0 |
| Furthermore , Pin 1 can interact directly with the C terminal domain of CK 2 alpha that contains residues that are phosphorylated in vitro by p 34 ( Cdc 2 ) and in mitotic cells . ^^^ |
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| Interacting proteins: P06493 and P68400 |
Pubmed |
SVM Score :0.0 |
| We now show that N terminal to the NLS domain of pro IL 16 are protein kinase CK 2 substrate and cdc 2 kinase substrate sites which , along with the NLS , constitute a dual phosphorylation regulated CcN motif which regulates nuclear localization of pro IL 16 . ^^^ |
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| Interacting proteins: P06493 and P68400 |
Pubmed |
SVM Score :0.0 |
| The mSTI 1 protein can be phosphorylated in vitro by cell cycle kinases proximal to a putative nuclear localization signal ( NLS ) , which substantiated a predicted casein kinase 2 ( CKII ) cdc 2 kinase NLS ( CcN ) motif at position 180 239 and suggested that mSTI 1 might move between the cytoplasm and the nucleus under certain cell cycle conditions . ^^^ |
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| Interacting proteins: P06493 and P68400 |
Pubmed |
SVM Score :0.0 |
| In addition , the absence of putative phosphorylation sites for Cdc 2 kinase on both alpha and beta subunits suggested an important role for CK 2 in regulating meiotic cell cycle in C . intestinalis oocytes . . ^^^ |
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| Interacting proteins: P06493 and P68400 |
Pubmed |
SVM Score :0.0 |
| We measured IC 50 values for emodin on the activities of several Ser / Thr protein kinases , including cAMP dependent protein kinase ( PKA ) , protein kinase C ( PKC ) , cdc 2 , casein kinases 1 ( CKI ) and CKII . ^^^ |
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| Interacting proteins: P06493 and P68400 |
Pubmed |
SVM Score :0.0 |
| Normally excluded from the LMW MAP2c , exon 10 harbors a putative CcN motif , comprising a nuclear localization sequence ( NLS ) flanked by regulatory phosphorylation sites for protein kinase CK 2 and cdc 2 kinase . ^^^ |
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| Interacting proteins: P06493 and P68400 |
Pubmed |
SVM Score :0.0 |
| Bacterially expressed domain E of Xenopus occludin ( amino acids 247 493 ) was a good substrate for protein kinase CK 2 ( stoichiometry 10 . 8 % , Km 8 . 4 microM ) but not for CK 1 kinase , protein kinase A , cdc 2 kinase , MAP kinase or syk kinase . ^^^ |
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| Interacting proteins: P06493 and P68400 |
Pubmed |
SVM Score :0.0 |
| An interesting aspect of nucleolin action is that it is a target for regulation by proteolysis , methylation , ADP ribosylation , and phosphorylation by CKII , cdc 2 , PKC 11 , cyclic AMP dependent protein kinase , and ecto protein kinase . ^^^ |
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| Interacting proteins: P06493 and P68400 |
Pubmed |
SVM Score :0.0 |
| Additionally , transfection of Cdc 2 with a mutation at Ser ( 39 ) to Ala , which is the CK 2 phosphorylation site , partially inhibits cell cycle progression in G ( 1 ) to G ( 2 ) phase following 6 TG treatment . ^^^ |
|
| Interacting proteins: P06493 and P68400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P68400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P68400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P68400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P68400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P68400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P68400 |
Pubmed |
SVM Score :0.0 |
| NA |
|