| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| Effects of phosphorylation by CAK on cyclin binding by CDC 2 and CDK 2 . ^^^ One complex ( CDC 2 cyclin A ) forms with high affinity only after CAK mediated phosphorylation of CDC 2 at the activating threonine residue . ^^^ |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| We report here that two polypeptides of the purified mammalian TFIIH are the MO15 / Cdk7 kinase and cyclin H subunits of the Cdk activating kinase Cak , previously identified as a positive regulator of Cdc 2 and Cdk 2 . ^^^ |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| This step is catalyzed by a cdk activating kinase ( CAK ) functionally analogous to the enzyme which phosphorylates cdc 2 and cdk 2 at Thr 161 / 160 . ^^^ |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| The CAK holoenzyme activates complexes of CDK 2 and CDC 2 with various cyclins and also phosphorylates CDK 2 , but not CDC 2 , in the absence of cyclin . ^^^ |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| CDK activation is dependent on cyclin binding and phosphorylation of a conserved threonine ( T 161 in Cdc 2 ) mediated by the CDK activating kinase CAK . ^^^ |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| The catalytic domain of ( HS ) Cak 1 , defined by conserved kinase Subdomains 1 through 11 , exhibits considerable homology with ( HS ) Cdc 2 , suggesting that this kinase cascade involves closely related enzymes . ^^^ Immunological studies with anti Cak antibodies confirm the presence of specific immunoreactivity in highly purified preparations of the human Cdc 2 activating kinase . ^^^ |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| To determine if the Cdk2 / cyclin A complex could be activated further by phosphorylation of T 160 , complexes were treated with Cdc 2 activating kinase ( CAK ) , purified approximately 12 , 000 fold from Xenopus eggs . ^^^ |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| Together these data suggest that the Crk 1 Mcs2 complex is a CAK that interacts genetically with Cdc 2 in fission yeast . . ^^^ |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| Differential expression of a CAK ( cdc 2 activating kinase ) like protein kinase , cyclins and cdc 2 genes from rice during the cell cycle and in response to gibberellin . ^^^ The R 2 gene from rice has 55 % sequence identity to the cdc 2 activating kinase ( CAK ) family of protein kinases which have been shown to phosphorylate and thereby activate cdc 2 protein kinases in animals and yeast . ^^^ |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| Cdc 2 cyclin complexes isolated from the arrested cells could be activated in vitro by recombinant CAK , whereas complexes from wild type cells or either of the single mutants were refractory to activation . ^^^ |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| Cyclin dependent kinase 7 ( CDK 7 ) is the catalytic subunit of the metazoan CDK activating kinase ( CAK ) , which activates CDKs , such as CDC 2 and CDK 2 , through phosphorylation of a conserved threonine residue in the T loop . ^^^ |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| Mcs 6 , human Cdk 7 and budding yeast Cak 1 were all active as Caks for Cdc 2 when expressed in fission yeast . ^^^ Biochemical experiments supported these genetic results : budding yeast Cak 1 could bind and phosphorylate Cdc 2 from fission yeast lysates , whereas fission yeast Csk 1 could not . ^^^ |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| Finally , neither cell cycle arrest nor CAK failure is recapitulated by a second mutation in mcs 6 13 that prevents Mcs 6 activation by Csk 1 , indicating that Csk 1 activates Cdc 2 directly in vivo . ^^^ |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| The Mcs 6 CDK together with its cognate cyclin Mcs 2 represents the CDK activating kinase ( CAK ) of fission yeast Cdc 2 . ^^^ |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| The fission yeast Mcs 6 Mcs2 Pmh 1 complex , homologous to metazoan Cdk 7 cyclin H Mat 1 , has dual functions in cell division and transcription : as a partially redundant cyclin dependent kinase ( CDK ) activating kinase ( CAK ) that phosphorylates the major cell cycle CDK , Cdc 2 , on Thr 167 ; and as the RNA polymerase ( Pol ) 2 carboxyl terminal domain ( CTD ) kinase associated with transcription factor ( TF ) IIH . ^^^ |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| The Cyclin dependent kinase ( CDK ) Activating Kinase ( CAK ) is responsible for the activating phosphorylation of CDK 1 , CDK 2 , CDK 4 and CDK 6 and regulation of the cell cycle . ^^^ |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| We report here the characterization of PNQALRE ( also known as CCRK or p 42 ) , a member of the mammalian CDK family most similar to the cell cycle effectors Cdk 1 and Cdk 2 and to the CAK , Cdk 7 . ^^^ |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| The addition of PfMAT 1 stimulated RNA polymerase 2 CTD phosphorylation and had no effect on the inability of Pfmrk to phosphorylate PfPK 5 , a putative CDK 1 homolog , which suggests that Pfmrk may be a CTD kinase rather than a CAK . ^^^ |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| The human Cdk activating protein kinase ( Cak 1 ) is itself a Cdc 2 related cyclin dependent protein kinase that associates with cyclin H . ^^^ Immunoaffinity purified Cak 1 phosphorylated monomeric Cdc 2 and Cdk 2 , but not Cdk 4 ; the phosphorylation of both Cdc 2 and Cdk 2 were increased in the presence of recombinant cyclin A . ^^^ These studies indicate that the Cak 1 catalytic subunit , like Cdc 2 and Cdk 2 , associates with multiple regulatory partners and suggests that subunit composition may be an important determinant of this multifunctional enzyme . . ^^^ |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| This stimulation was dependent on the activating phosphorylation of p 34 ( cdc 2 ) on Thr 161 , which follows cyclin binding and is mediated by the cdk activating kinase . ^^^ |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| The novel serine / threonine kinases , designated STK 1 and STK 2 , were homologous to the human cdc 2 and the Aspergillus nimA genes . ^^^ |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| In the absence of phosphorylation , cdk 2 , cdc 2 , and cdk 7 form highly stable complexes with their `` natural ' ' cyclin partners with dissociation constants in the nanomolar range . ^^^ In contrast , nonphosphorylated cdc 2 cyclin H , cdk 2 cyclin H , and cdk 7 cyclin A complexes present a 25 fold lower stability . ^^^ |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| We now demonstrate that the abnormal fiber regions in MFM immunoreact strongly for ( a ) CDC 2 kinase , the mitotic kinase that phosphorylates and disassembles intermediate filaments ; ( b ) cyclin dependent kinases CDK 2 , CDK 4 , and CDK 7 , which are involved in regulation of the cell cycle ; ( c ) lamin B , which normally supports the inner nuclear membrane ; and ( d ) the nuclear matrix associated protein . ^^^ |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| Immunoblotting experiments using monoclonal antibodies against cdc 2 , cyclin B , and cdk 7 ( an activator of cdc 2 ) have revealed that the concentrations of cdc 2 and cdk 7 are almost constant during oogenesis . ^^^ These results , which are identical to those in goldfish , strongly suggest that cyclin B is synthesized from stored ( masked ) mRNA after 17alpha , 20beta DP stimulation and that its binding to the preexisting cdc 2 allows cdk 7 to activate MPF . ^^^ These results indicate that , as in fully grown oocytes , growing oocytes are already equipped with the catalytic subunit of MPF ( cdc 2 ) and its activator ( cdk 7 ) and that the appearance of the regulatory subunit of MPF ( cyclin B ) is sufficient for initiating maturation . ^^^ |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| The regulation and functions of cdk 7 . cdk 7 started its life rather anonymously as a kinase called MO 15 , identified during a search for cDNA ' s which encode protein kinases related to cdc 2 . ^^^ |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| Expression of cyclin H protein , detected by western blotting of hippocampal tissue , was increased after global ischemia , but expression of cyclins B 1 and D 1 and other related cell cycle genes ( Cdk 7 and Cdc 2 ) was not increased . ^^^ |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| We also demonstrate that although the 1 , 25D ( 3 ) treated cells exhibit elevated levels of cyclin B 1 , Cdc25C , and Cdk 7 , which are positive regulators of the G ( 2 ) / M traverse , these cells have decreased protein levels of p 34 ( cdc 2 ) and decreased p 34 ( cdc 2 ) kinase activity . ^^^ |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| In addition , a fraction of cdc 2 protein from infected cells exhibited altered electrophoretic mobility in denaturing gels . ( 3 ) The levels of cdk 7 or myt 1 proteins remained relatively constant throughout infection , whereas the level of wee 1 was significantly decreased . ( 4 ) cdc 25C formed novel bands characterized by slower electrophoretic mobility that disappeared after treatment with phosphatase . ^^^ |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| Several CTD kinases are members of the cyclin dependent kinase ( cdk ) superfamily , including p34cdc2 ( cdk 1 ) , cdk 7 , cdk 8 , and cdk 9 . ^^^ |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| Enzymatic activities of cyclin D 1 , E , A , Cdk 4 , Cdk 6 , Cdc 2 , Cdk 7 , and Wee 1 kinase were measured by in gel kinase assay . ^^^ |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| The protein levels of cyclins D 1 , E , A , and H , and of cyclin dependent kinase 1 ( Cdk 1 ) , Cdk 2 , Cdk 4 , Cdk 6 , and Cdk 7 in HCC and in surrounding nontumorous cirrhosis were determined by Western blot . ^^^ The enzymatic activities of cyclins D 1 , E , A , Cdk 1 , Cdk 4 , Cdk 6 , Cdk 7 , and Wee 1 were measured using in vitro kinase assays . ^^^ The kinase activities of Cdk 6 , Cdk 7 , and Cdk 1 did not differ between HCC and surrounding cirrhotic tissues . ^^^ |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| Kinase assays revealed that the reduction of p 34 ( CDC 2 ) activity by resveratrol was mediated through the inhibition of CDK 7 kinase activity , while CDC25A phosphatase activity was not affected . ^^^ In addition , resveratrol treated cells were shown to have a low level of CDK 7 kinase Thr ( 161 ) phosphorylated p 34 ( CDC 2 ) . ^^^ |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| Moreover , Xpd is downregulated at the beginning of mitosis when Cdk 1 , a cell cycle target of Cdk 7 , is most active . ^^^ |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| Activation of cyclin B 1 associated CDC 2 kinase seems to be an important G2 / M event required for paclitaxel induced apoptosis , and this activation of cyclin B1 / CDC 2 kinase could be attributed to the increased activity of CDK 7 kinase . . ^^^ |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| Roscovitine ( Rosco ) is a purine derivative that inhibits cyclin dependent kinase 1 ( cdk 1 ) , cdk 2 , cdk 5 , cdk 7 , and cdk 9 , which are key regulators of the cell cycle and transcription . ^^^ |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| Rosco preferentially inhibits CDKs involved in cell cycle regulation ( CDK 1 , 2 , and 7 ) or neuronal functions ( CDK 5 ) , whereas Flavo preferentially inhibits CDKs involved in cell cycle ( CDK 1 , 2 , 4 , 7 ) or transcription ( CDK 7 , and 9 ) . ^^^ |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| In contrast , roscovitine and other purine PCIs inhibit with high potency only CDK 1 , CDK 2 , CDK 5 , and CDK 7 , and they specifically inhibit the expression of viral but not cellular genes . ^^^ |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| We demonstrated first that IE 63 was phosphorylated on Ser 224 in vitro by CDK 1 and CDK 5 but not by CDK 2 , CDK 7 , or CDK 9 . ^^^ |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| Among them , compound 20 displayed high inhibitory potency at CDK 1 ( IC ( 50 ) = 0 . 021 microM ) , CDK 2 , and CDK 5 and submicromolar potency at CDK 4 , CDK 6 , and CDK 7 . ^^^ |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| The latter cells were the most responsive to betulinic acid , showing a selective decline in the cdk 4 protein , without a comparable change in other key cell cycle proteins like cdc 2 , cdk 2 , cdk 7 or cyclin A , prior to apoptosis associated PARP fragmentation . ^^^ |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| Furthermore , down regulation of CDK 2 , CDK 4 , and cyclin D 1 was observed in PC 3 cells treated with 1 , 000 nmol / L of MONCPT , whereas overexpression of CDK 7 , CDK 1 , and cyclin B 1 was seen in PC 3 cells treated with 10 and 100 nmol / L of MONCPT . ^^^ |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| Roscovitine arrests cell cycle progression in G ( 1 ) and in G ( 2 ) phase by inhibiting CDK 2 and CDK 1 , and possibly CDK 7 and CDK 9 . ^^^ |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P50613 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|