| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.70833215 |
| It was found that 14 3 3rho was not expressed in H 1299 cells , and that although CDKN1A did associate with CDC 2 to form a complex , the level of CDKN1A associated with CDC 2 was not increased in response to radiation or to PD 0166285 . 0.70833215^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| The p21WAF1 , also known as CIP 1 or SDI 1 , was the first reported CDKI : it ' s expression is induced by wild type p 53 . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| One mechanism is via ErbB 2 mediated upregulation of p 21 ( Cip 1 ) , which inhibits Cdc 2 . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| Three lines mildly induced CdkI p 21 ( WAF 1 ) expression in response to TGF beta . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| The membranes were stained with amido black , scanned and tested separately with the nine monoclonal antibodies p 53 , c myc , PCNA , MEK 1 , pan ras , Cip 1 , Cdc 2 , Kip 1 , and TCTP . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| All these events are totally inhibited by the cdk inhibitor p 21 ( Cip 1 ) , demonstrating that Plx 1 kinase activation depends on Cdc 2 kinase activity . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| After microinjection of p 21 ( cip 1 ) , progesterone fails to induce the activation of MAPK or p 34 ( cdc 2 ) , and Mos does not accumulate . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| To better understand the role of Cdc 2 , MPF activity was specifically inhibited by injection of the CDK inhibitor , Cip 1 . ^^^ When Cip 1 is injected at germinal vesicle breakdown ( GVBD ) time , Cdc 25 and Plx 1 are both dephosphorylated and Cdc 2 is rephosphorylated on tyrosine . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| Of the cell cycle control proteins , PCNA , Cdk 2 , and cyclin A were detected at high concentrations ; cdc 2 , Cdk 4 , and cyclin B were detected at very low concentrations ; while cyclin D 1 , cyclin D 3 , Cip 1 , and Kip 1 were not detected . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| We demonstrate that the up regulation of the CDK inhibitors p 27 ( KIP 1 ) and p 21 ( CIP 1 ) is likely responsible for the inhibition of CDK 2 while inhibition of CDK 1 was rather due to the phosphorylation of the kinase on Tyr 15 residue . . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| These events were associated with increases in the protein levels of cyclin B 1 , cyclin D 1 , cyclin E , cyclin dependent kinase 1 , cyclin dependent kinase 2 , proliferating cell nuclear antigen , and p 21 ( Cip 1 ) in vivo and in vitro , whereas inhibition of the PI 3 kinase signaling pathway with either rapamycin or wortmannin blocked the upregulation of these cell cycle proteins , but not mRNA , and arrested the cells in vitro before S phase . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| Cdc 2 and Cdk 2 cyclin complexes can be inactivated by phosphorylation on the catalytic cleft located Thr 14 and Tyr 15 residues or by association with inhibitory subunits such as p 21 ( Cip 1 ) . ^^^ Moreover , RINGO bound Cdc 2 and Cdk 2 are both less susceptible to inhibition by p 21 ( Cip 1 ) , whereas the Thr 14 / Tyr 15 kinase Myt 1 can negatively regulate the activity of Cdc 2 RINGO with reduced efficiency . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| The molecular mechanisms underlying this G ( 1 ) phase arrest appear to be due to the induction of the cyclin dependent kinase inhibitors ( CDKI ) p 15 ( INK 4 ) , p 21 ( Cip 1 ) , and p 27 ( Kip 1 ) , which are known to bind and inactivate cyclinD / CDK4 / 6 and the cyclin E / CDK2 kinase complexes . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| Herceptin treatment down regulated ErbB 2 , reduced the inhibitory phosphorylation of Cdc 2 on Tyr 15 , and down regulated the expression of p 21 ( Cip 1 ) , a Cdc 2 inhibitor . ^^^ Herceptin plus Taxol treatment led to higher levels of p 34 ( Cdc 2 ) kinase activity and apoptosis in ErbB 2 overexpressing breast cancer cells , which is likely attributable to inhibition of Cdc 2 Tyr 15 phosphorylation and p 21 ( Cip 1 ) expression . ^^^ Because significant dephosphorylation of Cdc 2 Tyr 15 and down regulation of p 21 ( Cip 1 ) occur at least 24 h after Herceptin treatment , we investigated whether 24 h Herceptin pretreatment will render ErbB 2 overexpressing breast cancer cells more sensitive to Taxol induced apoptosis compared with the simultaneous treatment of Herceptin plus Taxol . ^^^ Thus , Herceptin treatment of ErbB 2 overexpressing cells can inhibit ErbB 2 mediated Cdc 2 Tyr 15 phosphorylation and p 21 ( Cip 1 ) up regulation , which allows effective p 34 ( Cdc 2 ) activation and induction of apoptosis upon Taxol treatment . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| CGP74514A treatment ( 5 microM ; 18 hr ) resulted in increased p 21 ( CIP 1 ) expression , p 27 ( KIP 1 ) degradation , diminished E2F1 expression , and dephosphorylation of p 34 ( CDC 2 ) . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| The protein or mRNA levels of the cyclin dependent kinase inhibitors ( cdki ) p 21 ( Cip 1 ) , p 27 ( Kip 1 ) were unaffected by Dex treatment of ASM cells treated with mitogens . ^^^ The resistance of EGF induced proliferation to inhibition by glucocorticoids is not associated with a failure to regulate cyclin D 1 induction , nor does it appear to be explained by differential regulation of the levels of the cdki ' s , p 21 ( Cip 1 ) and p 27 ( Kip 1 ) . . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| The underlying molecular mechanism is that ErbB 2 inhibits p 34 ( Cdc 2 ) activation , which is required for taxol induced apoptosis , by up regulating p 21 ( Cip 1 ) and by hyperphosphorylating p 34 ( Cdc 2 ) on tyrosine 15 . ^^^ However , the relation between ErbB 2 , p 21 ( Cip 1 ) , and p 34 ( Cdc 2 ) in patients with breast carcinoma remains elusive . ^^^ METHODS : Formalin fixed , paraffin embedded , 5 microm thick tissue sections from 107 patients with invasive breast carcinoma were immunostained using specific antibodies against ErbB 2 , p 21 ( Cip 1 ) , and phosphorylated tyrosine ( Tyr ) 15 of p 34 ( Cdc 2 ) . ^^^ ErbB 2 expression levels , p 21 ( Cip 1 ) expression levels , and phosphorylation status on Tyr 15 of p 34 ( Cdc 2 ) were analyzed for correlations with clinicopathologic parameters for the 107 patients and for correlations with disease free survival ( DFS ) in the 94 patients who were treated with the CMF regimen . ^^^ It is noteworthy that p 21 ( Cip 1 ) expression and p 34 ( Cdc 2 ) Tyr 15 phosphorylation were correlated significantly and positively with ErbB 2 expression ( P < 0 . 01 ) . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| In U 937 cells , synergistic interactions between MG 132 and flavopiridol were associated with multiple perturbations in expression / activation of signaling and survival related proteins , including downregulation of XIAP and Mcl 1 , activation of JNK and p 34 ( cdc 2 ) , and diminished expression of p 21 ( CIP 1 ) . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| Coadministration of 17 AAG blocked SAHA mediated induction of the cyclin dependent kinase inhibitor p 21 ( CIP 1 ) and resulted in reduced expression of p 27 ( KIP 1 ) and p 34 ( cdc 2 ) . 17 AAG / SAHA treated cells also displayed down regulation of the antiapoptotic protein Mcl 1 and evidence of Bcl 2 cleavage . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| IFIXalpha 1 appears to suppress the growth of breast cancer cells in a pRB and p 53 independent manner by increasing the expression of the cyclin dependent kinase inhibitor p 21 ( CIP 1 ) , which leads to the reduction of the kinase activity of both Cdk 2 and p 34 ( Cdc 2 ) . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| In this review , we discuss recent results indicating a critical role for the cyclin dependent kinase inhibitors ( CDKI ) p 27 ( Kip 1 ) and p 21 ( Cip 1 ) in establishing Sertoli cell number , testis weight , and DSP , and the ability of thyroid hormone to modulate these CDKIs . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| Here , we show that both early passage Foxm 1 ( ) ( / ) ( ) mouse embryonic fibroblasts ( MEFs ) and human osteosarcoma U2OS cells depleted of FoxM 1 protein by small interfering RNA fail to grow in culture due to a mitotic block and accumulate nuclear levels of cyclin dependent kinase inhibitor ( CDKI ) proteins p 21 ( Cip 1 ) and p 27 ( Kip 1 ) . ^^^ We also identify the mechanism by which FoxM 1 deficiency causes elevated nuclear levels of the CDKI proteins p 21 ( Cip 1 ) and p 27 ( Kip 1 ) . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| Study of the key regulators of cell cycle progression by Western blot analysis showed that the expression of the cyclin dependent kinase inhibitor ( CDKI ) p 27 ( kip 1 ) , but not that of p 21 ( cip 1 ) , was enhanced , whereas that of c Myc , cyclin E , cyclin D 2 , and cyclin dependent kinases 2 and 4 ( CDK 2 and CDK 4 ) was decreased when these cells were treated with TZD 18 ( 10 or 20 microM ) . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVE : It is known that the cyclin dependent kinase inhibitor ( CDKI ) gene p 21 ( Cip 1 ) suppresses rheumatoid inflammation by down modulating type 1 interleukin 1 receptor ( IL 1RI ) expression and inhibiting JNK activity . ^^^ Unlike p 21 ( Cip 1 ) , neither CDKI gene inhibited IL 1RI or JNK . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| We demonstrate by western analysis that the expression levels of TP 53 ( formerly known as p 53 ) , CDKN1A ( formerly known as p21Waf1 ) , CDC 2 ( formerly known as p34cdc2 ) , CCNB 1 ( cyclin B 1 ) and RAD 51 are significantly modulated in confluent , density inhibited human diploid cell populations exposed to doses where only a small fraction of the nuclei are actually traversed by an alpha particle track . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| Furthermore , the addition of supernatants from alpha irradiated cells to unirradiated cells decreases cellular levels of TP 53 and CDKN1A and increases CDC 2 and proliferating cell nuclear antigen in the latter . ^^^ As well , the proliferating cell nuclear antigen and CDC 2 increases that occur along with the decreased TP53 / CDKN1A bystander effect also would expectedly favor enhanced cell growth . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| In addition , reduced cyclin D 1 , cyclin A and Cdc 2 levels and increased levels of Cdkn1a ( also known as p 21 ( Waf ) ) were observed in Hsp 25 transfected cells , which probably caused the reduction in cell growth . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| Most gene expression alterations were detected 24 h after paclitaxel administration and affected genes involved in various biological functions including cell cycle regulation and cell proliferation ( CDC 2 , CDKN1A , PLAB , and TOP2A ) , apoptosis ( BNIP 3 and PIG 8 ) , signal transduction and transcriptional regulation ( ARF 1 , ATF 2 , FOS , GNA 11 , HDAC 3 , MADH 2 , SLUG , and SPRY 4 ) , fatty acid biosynthesis and sterol metabolism ( FDPS , IDI 1 , LIPA , and SC5D ) , and IFN mediated signaling ( G1P3 , IFI 16 , IFI 27 , IFITM 1 , and ISG 15 ) . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| Loss of mitosis in tumor cells is associated with marked reduction of cyclin dependent kinase ( CDK ) 1 transcription and / or loss of its active form ( CDK 1 P Thr ( 161 ) ) , which is coincident with up regulation of CDKN1A , CDKN1B , and CDKN1C ( p 21 , p 27 , and p 57 ) . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| Interestingly , when Cdk 2 kinase activity is inhibited by the Cdk specific inhibitor , p21Cip1 , a block to mitosis occurs , and inactive Cdc 2 cyclin B accumulates . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| A recent study showed that mimosine might induce growth arrest by activating the expression of p21CIP1 , a cyclin dependent kinase inhibitor ( CDKI ) , and by inhibiting the activity of cyclin E associated kinases in human breast cancer cells . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| While SAHA induced the cyclin dependent kinase inhibitor p21CIP1 , antisense ablation of this CDKI increased , rather than decreased , SAHA related lethality . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| The role of p21CIP1 in the inhibition of CDK 1 was questionable , as we demonstrated that genistein impaired Tyr 15 dephosphorylation of CDK 1 and because CDK 1 cyclin B 1 complexes from treated cells could be reactivated upon exposure to CDC 25 phosphatase . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| The functional role of the cyclin dependent kinase inhibitor ( CDKI ) p21CIP1 in differentiation of human myelomonocytic leukemia cells ( U 937 ) exposed to low concentrations of the antimetabolite 1 beta D arabino furanosylcytosine ( ara C ) was examined utilizing a cell line stably expressing a p21CIP1 antisense construct . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| To clarify the cell cycle status and role of cyclin dependent kinase inhibitors ( CDKI ) for cell cycle control in the taste buds , we analyzed cell proliferation activity using bromodeoxyuridine ( BrdU ) and Ki 67 immunostainings and the expression of the Cip / Kip family of CDKI ( p21Cip1 , p27Kip1 , and p57Kip2 ) in the circumvallate papillae of mouse and hamster . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| In particular , augmentation of CDK inhibitors p21cip1 and p27kip1 and Wee 1 kinase and diminution of CDC25C phosphatase coincided with induced Tyr 15 phosphorylation and inhibition of CDC 2 . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| This was associated with the earlier expression of the cell cycle promoting cyclin A 2 , cyclin E , cyclin B 1 , cyclin F , and cyclin dependent kinase 1 ( Cdk 1 ) genes and diminished protein levels of Cdk inhibitor p21Cip1 . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| Adenoviral p 57 overexpression induced growth arrest in prehypertrophic epiphyseal chondrocytes in a dose dependent manner but was not sufficient for the induction of collagen type 10 , either alone or when coexpressed with the related CDKI p21Cip1 . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| P21Cip1 , cdk 1 , cdk 2 , and cyclin E mRNA expression increased coincident with S phase accumulation in HT 29 cells treated with ZD 9331 or 5fluorouracil / leucovorin , as demonstrated by cDNA microarray analyses . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| Specifically , BAY reduced the protein expression of p21Cip1 as well as cyclin D 1 , and inhibits the expression of cdc 2 ( cdk 1 ) . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| The p 53 protein directly regulates the expression of the WAF 1 ( wild type p 53 activated fragment 1 ) protein which is a cyclin dependent kinase inhibitor ( CDK 1 ) . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| The fused peptides prevented growth at concentrations which inhibited cyclin dependent kinase 2 and cdc 2 activity , thus demonstrating that the peptides act by mimicking the action of p 21 ( WAF 1 ) on kinases . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| The early response to RAS activation in thyroid epithelial cells showed two features not seen in fibroblasts : ( 1 ) a marked decrease in expression of the cyclin dependent kinase inhibitor ( CDKI ) p 27 ( kip 1 ) and ( 2 ) the absence of any induction of p 21 ( waf 1 ) . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| GADD 45 can interact in vivo with the G ( 2 ) cell cycle specific kinase , Cdc 2 , proliferating cell nuclear antigen ( PCNA ) , and the cell cycle kinase inhibitor p 21 ( waf 1 ) . ^^^ This mutant also lacked an ability to bind to Cdc 2 , PCNA , and p 21 ( waf 1 ) in vivo . ^^^ Alanine substitutions in the region abolished GADD 45 induction of a G ( 2 ) / M arrest and its inactivation of the Cdc 2 kinase but not its binding to Cdc 2 , PCNA , or p 21 ( waf 1 ) . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| The CdKI ' s p 21 ( waf 1 ) and p 16 ( Ink 4a ) were found overexpressed in H ( 2 ) O ( 2 ) induced premature senescence , while p 19 ( Ink 4d ) and p 27 ( Kip 1 ) were repressed . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| In cotransfection studies , cyclin A Cdk 1 inhibited CDP / Cux stable DNA binding and prevented repression of the p 21 ( WAF 1 ) reporter . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| Such inhibition is apparently mediated by up regulation of the cyclin dependent kinase 1 p 21 ( Waf 1 ) and inhibition of cyclin dependent kinase 2 activity . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| A p 21 ( waf 1 ) independent pathway for inhibitory phosphorylation of cyclin dependent kinase p 34 ( cdc 2 ) and concomitant G ( 2 ) / M arrest by the chemopreventive flavonoid apigenin . ^^^ Apigenin treatment decreased the protein level of p 34 ( cdc 2 ) , and p 34 ( cdc 2 ) kinase activity was inhibited in both p 21 ( waf 1 ) ( + / + ) and p 21 ( waf 1 ) ( / ) cell lines by approximately 40 % . ^^^ These results supported the conclusion that G ( 2 ) / M arrest induced by apigenin was accompanied by inhibition of the p 34 ( cdc 2 ) cyclin dependent kinase protein level and activity in a p 21 ( waf 1 ) independent manner . . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| However , aphidicolin synchronized HN 12 cells released in the presence of perifosine ( 10 microM ) demonstrated increased expression of total p 21 ( WAF 1 ) and increased association of p 21 ( WAF 1 ) with cyclin cdk complexes resulting in reduced cdc 2 activity . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| We have investigated the effect of etoposide schedule on cell cycle distribution , apoptosis and p 21 ( waf 1 ) and cdk 1 ( p 34 ) status in two bcr abl positive chronic myeloid leukaemia ( CML ) cell lines ( K 562 and KU 812 ) and two small cell lung cancer ( SCLC ) cell lines ( H 69 and GLC 4 ) . ^^^ Although K 562 and KU 812 cells are null p 53 , the arrest in G2 / M during drug exposure was associated with increased p 21 ( waf 1 ) and a decrease in cdk 1 ( both P < 0 . 001 compared with controls ) . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| Cyclin dependent kinase inhibitor ( CdKI ) p 21 ( Waf 1 ) is overexpressed in H2O2 and tert butylhydroperoxide induced premature senescence , likely explaining in part the hypophosphorylation of the retinoblastoma protein . p 21 ( Waf 1 ) is known to inhibit the kinase activity of the cyclin dependent kinase ( CdK ) 4 and 6 cyclin complexes . ^^^ This CdK 2 mediated effect should be considered in addition to the inhibition of cyclin D CdK 4 and 6 complexes by CdKI p 21 ( Waf 1 ) . . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| The array data enabled us to focus on the role of potential key genes ( e . g . , GADD 153 , CHK 2 , p 21 ( WAF 1 ) , cyclin A , CDK 1 , and DHFR ) that might be targets of MSA in impeding cell cycle progression . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| AIMS : To look for correlations between expression of cell cycle regulatory proteins p 34 ( cdc 2 ) , p 21 ( WAF 1 ) , and p 53 in node negative invasive ductal breast carcinoma , or between these proteins and clinicopathological parameters , and to assess their prognostic value . ^^^ RESULTS : Nuclear and cytoplasmic p 34 ( cdc 2 ) expression was seen in 80 and 62 tumours , respectively ; nuclear expression was seen in adjacent benign epithelium in 12 cases . p 21 ( WAF 1 ) and p 53 were positive in 48 and 21 tumours , respectively . ^^^ CONCLUSIONS : p 34 ( cdc 2 ) , p 21 ( WAF 1 ) , and p 53 expression does not predict outcome in node negative breast carcinoma , although p 34 ( cdc 2 ) expression in benign tissue is related to prognosis . ^^^ The association between p 34 ( cdc 2 ) and p 53 implicates p 53 in G 2 M cell cycle checkpoint control , possibly via mediators unrelated to p 21 ( WAF 1 ) . . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| These nine proteins ( DP 1 , cdc25A , cyclin A , cyclin B ( 1 ) , cyclin D ( 1 ) , cdk 1 , cdk 5 , p 21 ( WAF 1 ) , and GADD 153 ) have been reported previously by our laboratory to be modulated by MSA in human breast and prostate cancer cells . ^^^ |
|
| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| This normal cell line DNA demethylase activity associates with PCNA immune complex that is inhibited by CDKI proteins p 21 ( waf 1 ) / Gadd45alpha and Gadd45beta . ^^^ This cancer cell line DNA demethylase activity is not associated with PCNA immune complex and is not inhibited by CDKI proteins p 21 ( waf 1 ) / Gadd45alpha and Gadd45beta . ^^^ |
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| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| To elucidate that the cell cycle inhibitory effect of GJS stems from its capability of transcriptional regulation of the cell cycle controlling genes , we investigated mRNA expression of p 53 , Waf 1 , PCNA , Cyclin D 1 , Cdc 2 , Histone H 3 , c Myc , and c Fos . ^^^ |
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| Interacting proteins: P38936 and P06493 |
Pubmed |
SVM Score :0.0 |
| Expression of p 53 , p 21 ( WAF 1 ) , Bax , Bak , bcl 2 , as well as total and phosphorylated Cdc 2 in the absence and presence of olomoucine , a phosphorylated Cdc 2 kinase inhibitor , was then determined . ^^^ |
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