| Interacting proteins: P30305 and P06493 |
Pubmed |
SVM Score :0.0 |
| A dual specificity phosphatase Cdc25B is an unstable protein and triggers p 34 ( cdc 2 ) / cyclin B activation in hamster BHK 21 cells arrested with hydroxyurea . ^^^ |
|
| Interacting proteins: P30305 and P06493 |
Pubmed |
SVM Score :0.0 |
| Microinjection of anti cdc25A antibodies into HeLa cells causes their arrest in mitosis . cdc25A and cdc25B display endogenous tyrosine phosphatase activity that is stimulated several fold , in the absence of cdc 2 , by stoichiometric addition of either cyclin B 1 or B 2 but not A or D 1 . ^^^ |
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| Interacting proteins: P30305 and P06493 |
Pubmed |
SVM Score :0.0 |
| Northern and Western blotting detection reveals that unlike cdc25B , cdc25C and cdc 2 , cdc25A is predominantly expressed in late G 1 . ^^^ |
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| Interacting proteins: P30305 and P06493 |
Pubmed |
SVM Score :0.0 |
| We found that , like CDC 2 , CDK 2 is also phosphorylated on Thr 14 and Tyr 15 and that treatment of cyclin A or cyclin E immunoprecipitates with bacterially expressed Cdc25M2 ( the mouse homolog of human CDC25B ) increased the histone H 1 kinase activity of these immune complexes 5 to 10 fold . ^^^ |
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| Interacting proteins: P30305 and P06493 |
Pubmed |
SVM Score :0.0 |
| These data suggested that the PKC pathway negatively regulates the G2 / M transition and that the inhibition of cdc 2 kinase by the reduction in the levels of cdc25B and cyclin B may contribute to this effect . . ^^^ |
|
| Interacting proteins: P30305 and P06493 |
Pubmed |
SVM Score :0.0 |
| We now report further characterization of cyclin B 1 gene expression , as well as that of CDK 1 and cdc25B , in this unique in vivo model of cell proliferation . ^^^ By Northern blot analysis , single transcripts for CDK 1 and cdc25B were detected , and the temporal expression of both transcripts during liver regeneration mirrored that of cyclin B 1 . ^^^ |
|
| Interacting proteins: P30305 and P06493 |
Pubmed |
SVM Score :0.0 |
| K252a did not inhibit the expression of genes involved in mitosis such as cyclin B , cdc25B and cdc 2 , in the first round S phase . ^^^ |
|
| Interacting proteins: P30305 and P06493 |
Pubmed |
SVM Score :0.0 |
| Phosphorylation of human CDC25B phosphatase by CDK 1 cyclin A triggers its proteasome dependent degradation . ^^^ These results indicate that CDK 1 cyclin A phosphorylation targets CDC25B for degradation and that this might be an important component of cell cycle regulation at the G2 / M transition . . ^^^ |
|
| Interacting proteins: P30305 and P06493 |
Pubmed |
SVM Score :0.0 |
| In both cases , CDK 1 from arrested cells could be reactivated both in vitro by dephosphorylation by recombinant Cdc25B phosphatase and in vivo by caffeine . ^^^ |
|
| Interacting proteins: P30305 and P06493 |
Pubmed |
SVM Score :0.0 |
| Purified , N terminally truncated forms of cdc25B did not induce GVBD , even though many had phosphatase activity and activated cdc 2 in vitro . ^^^ |
|
| Interacting proteins: P30305 and P06493 |
Pubmed |
SVM Score :0.0 |
| The activity of cdc 2 associated kinase was decreased , which was accompanied by the up regulation of cdc 2 phosphorylation ( cdc 2 Tyr15 phosphorylation ) resulting from reduction of cdc25B and cdc25C phosphatases . ^^^ |
|
| Interacting proteins: P30305 and P06493 |
Pubmed |
SVM Score :0.0 |
| We report that overexpression of CDC25B or CDC25C overrides an established CDT induced G 2 cell cycle arrest and leads the cells to accumulate in an abnormal mitotic stage with condensed chromatin and high CDK 1 activity . ^^^ |
|
| Interacting proteins: P30305 and P06493 |
Pubmed |
SVM Score :0.0 |
| Providing an explanation for this , mRNA and protein levels of cdc25B , the trigger cdc 2 phosphatase , were significantly and specifically reduced . ^^^ |
|
| Interacting proteins: P30305 and P06493 |
Pubmed |
SVM Score :0.0 |
| CDC25B phosphatase plays a key role in controlling G 2 M progression by dephosphorylating two inhibitory residues of CDC 2 and also has been suggested to have an oncogenic property . ^^^ Moreover , induction of tyrosine phosphorylation of CDC 2 and reduction of CDC 2 kinase activity after irradiation was less significant in TE 8 CDC25B than in TE 8 neo . ^^^ These results indicate that cancer cells that overexpress CDC25B override G 2 M arrest by retaining CDC 2 kinase activity and undergo apoptosis after radiation . ^^^ |
|
| Interacting proteins: P30305 and P06493 |
Pubmed |
SVM Score :0.0 |
| Furthermore , restoring FoxM1B expression in old aged mice caused elevated levels of Cyclin B 1 , Cyclin B 2 , Cdc25B , Cdk 1 , and p55CDC mRNA as well as stimulating Cdc25B nuclear localization during liver regeneration , all of which are required for mitosis . ^^^ |
|
| Interacting proteins: P30305 and P06493 |
Pubmed |
SVM Score :0.0 |
| Moreover , the significant reduction in hepatocyte mitosis was associated with diminished mRNA levels and nuclear expression of Cdc25B phosphatase and delayed accumulation of cyclin B 1 protein , which is required for Cdk 1 activation and entry into mitosis . ^^^ |
|
| Interacting proteins: P30305 and P06493 |
Pubmed |
SVM Score :0.0 |
| Furthermore , increased Cdc25B expression and decreased Cdk 1 phosphorylation were observed in anti BPDE treated cells . ^^^ |
|
| Interacting proteins: P30305 and P06493 |
Pubmed |
SVM Score :0.0 |
| A 24 h treatment of PC 3 and LNCaP cells with 20 micro M AITC caused a significant decrease in the levels of proteins that regulate G ( 2 ) / M progression , including Cdk 1 ( 32 50 % reduction ) , Cdc25B ( 44 48 % reduction ) and Cdc25C ( > 90 % reduction ) . ^^^ |
|
| Interacting proteins: P30305 and P06493 |
Pubmed |
SVM Score :0.0 |
| Two CDC25B inhibitors reduced the growth of pancreatic cancer cell lines , resulting in the accumulation of phosphorylated CDC 2 and G2 / M arrest . ^^^ |
|
| Interacting proteins: P30305 and P06493 |
Pubmed |
SVM Score :0.0 |
| We report here that reducing cyclin A expression by RNA interference ( RNAi ) in primary human fibroblasts inhibited activation of Cdc25B and Cdc25C and dephosphorylation of Cdk 1 on tyrosine ( tyr ) 15 . ^^^ Cdk 2 dn induction could inhibit Cdc25B activity and foster Cdk 1 tyr phosphorylation within the S phase , temporally dissociating these events from Cdk 1 activation at mitosis . ^^^ In contrast , reducing Cdk 1 expression delayed mitotic entry without markedly impairing Cdc25B or Cdc25C activity . ^^^ |
|
| Interacting proteins: P30305 and P06493 |
Pubmed |
SVM Score :0.0 |
| Treatment of BxPC 3 cells with growth suppressive concentrations of BITC resulted in G ( 2 ) / M phase cell cycle arrest that was associated with a marked decline in protein levels of G ( 2 ) / M regulatory proteins including cyclin dependent kinase 1 ( Cdk 1 ) , cyclin B 1 and cell division cycle 25B ( Cdc25B ) . ^^^ |
|
| Interacting proteins: P30305 and P06493 |
Pubmed |
SVM Score :0.0 |
| Activation of centrosomal Cdk 1 in late prophase seemed to be mediated by cytoplasmic Cdc25B , whose activity is controlled by centrosome associated Chk 1 . ^^^ These results suggest that centrosome associated Chk 1 shields centrosomal Cdk 1 from unscheduled activation by cytoplasmic Cdc25B , thereby contributing to proper timing of the initial steps of cell division , including mitotic spindle formation . . ^^^ |
|
| Interacting proteins: P30305 and P06493 |
Pubmed |
SVM Score :0.0 |
| CDC25B that is responsible for the activation of CDK 1 cyclin B 1 is regulated by phosphorylation . ^^^ We show that accumulation of the S 353 phosphorylated form of CDC25B at the centrosome correlates with the relocalization of cyclin B 1 to the nucleus and the activation of CDK 1 at entry into mitosis . ^^^ These results strongly suggest that Aurora A mediated phosphorylation of CDC25B at the centrosome is an important step contributing to the earliest events inducing mitosis , upstream of CDK 1 cyclin B 1 activation . . ^^^ |
|
| Interacting proteins: P30305 and P06493 |
Pubmed |
SVM Score :0.0 |
| METHODS AND RESULTS : The mRNA transcript levels of CDC 2 , CCNB 1 , CCNB 2 , CDC25A , CDC25B , CDC25C and WEE 1 in the testes of 37 azoospermic patients were examined by quantitative real time polymerase chain reaction . ^^^ |
|
| Interacting proteins: P30305 and P06493 |
Pubmed |
SVM Score :0.0 |
| The specificity of BN 82685 towards the CDC 25 phosphatases is shown by an increase in cyclin dependent kinase 1 tyrosine 15 phosphorylation , by the reversion of the mitosis inducing effect of CDC25B overexpression in HeLa cells , and by the lack of a growth inhibitory effect in an assay based on the use of a CDC 25 independent fission yeast model . ^^^ |
|
| Interacting proteins: P30305 and P06493 |
Pubmed |
SVM Score :0.0 |
| Further , three dimensional time lapse microscopy and quantification of Cdk 1 phosphorylation versus cyclin B 1 levels in individual cells revealed that Cdc25A and B exert specific functions in the initiation of mitosis : Cdc25A may play a role in chromatin condensation , whereas Cdc25B specifically activates cyclin B 1 Cdk1 on centrosomes . . ^^^ |
|
| Interacting proteins: P30305 and P06493 |
Pubmed |
SVM Score :0.0 |
| Cdc25B is a dual specific phosphatase , which plays a pivotal role in the activation of cell cycle dependent kinase 1 ( Cdk 1 ) . ^^^ |
|
| Interacting proteins: P30305 and P06493 |
Pubmed |
SVM Score :0.0 |
| Cell cycle regulating gene CDC 2 and genes ( CDC25B and Tome 1 ) that control CDC 2 activity showed increased expression in the presence of siPTHrP . ^^^ Studies with PTHrP peptides 1 34 and 67 86 , forskolin , and a PTH 1 receptor ( PTH1R ) specific siRNA showed that PTHrP regulates CDC 2 and CDC25B , at least in part , via PTH1R in a cAMP independent manner . ^^^ Comparative expression analyses with 50 primary breast carcinomas revealed that the RNA level of ITGA 6 correlates with that of PTHrP , and higher CDC 2 and CDC25B values are found at low PTHrP expression . ^^^ |
|
| Interacting proteins: P30305 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30305 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30305 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30305 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30305 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30305 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30305 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30305 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30305 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30305 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|