Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.80681436
Cyclin A associates with both Cdc 2 and its homologue Cdk 2 . 0.80681436^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.73273071
These novel Mab did not interfere with the association of cdc 2 with cyclin A in vitro and efficient immunoprecipitation of a panel of cdc 2 mutant proteins suggests that the target epitopes may not involve amino acid residues essential for currently known cdc 2 functions . 0.73273071^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.75103576
Cdc 2 protein kinase is complexed with both cyclin A and B : evidence for proteolytic inactivation of MPF . 0.75103576^^^ Clam cdc 2 is found in association with both cyclin A and B , probably not as a trimolecular association , but as separate cdc2 / cyclin A and cdc2 / cyclin B complexes . 0.67733677^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.65783669
Goldfish cyclin A was associated with cdc 2 kinase in mature oocytes , but not with cdk 2 . 0.65783669^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :1.0288266
Cdc 2 associates with both cyclin A , which is synthesized at G 1 and S , and cyclin B , which is produced later during S and G 2 before G2 / M transition . 1.0288266^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :1.007288
RESULTS : We show that Cdc 2 co expressed with Cyclin A inhibits the S phase in Drosophila salivary glands and diploid abdominal histoblasts . 1.007288^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.6190489
Our previous studies have shown that inactivation of Drosophila lats leads to up regulation of cyclin A in the fly , and the human LATS 1 protein associates with CDC 2 in early mitosis in HeLa cells , suggesting that the lats gene family may negatively regulate cell proliferation by modulating CDC2 / Cyclin A activity . 0.6190489^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Hyperactivation of cdc 2 kinase by the addition of cyclin A has no effect on the activity or oscillatory behavior of Eg 1 . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Cyclin A binds both cdk 2 and cdc 2 , giving two distinct cyclin A kinase activities , one appearing in S phase , the other in G 2 . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
This purified kinase , and a closely related cyclin A associated cdc 2 like kinase , phosphorylated RPA p 34 on a subset of the chymotryptic peptides that were phosphorylated in vivo at the G 1 S transition . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Exit from metaphase of the cell cycle requires inactivation of MPF , a stoichiometric complex between the cdc 2 catalytic and the cyclin B regulatory subunits , as well as that of cyclin A cdc 2 kinase . ^^^ We show further that residue 161 of cdc 2 is phosphorylated in active cyclin A or cyclin B complexes at metaphase , whilst unassociated cdc 2 is not phosphorylated . ^^^ We conclude that both cyclin degradation and dephosphorylation of Thr 161 on cdc 2 , catalysed at least in part by type 1 phosphatase , are required to inactivate either cyclin B or cyclin A cdc 2 kinases and thus for cells to exit from M phase . . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
By contrast , other proline directed Ser / Thr kinases such as p 34 ( cdc 2 ) combined with cyclin A or B have only minor effects on tau phosphorylation . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Furthermore , cells synchronized in late G 1 by exposure to the drug mimosine contain active cyclin A / p34 CDC 2 kinase , indicating that p 34 CDC2 activation can occur before DNA synthesis begins . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Cyclin A cdc 2 kinase does not trigger but delays cyclin degradation in interphase extracts of amphibian eggs . ^^^ However , cyclin A cdc 2 kinase was not assayed in these early experiments . ^^^ Here we have shown that full length recombinant human cyclin A failed to induce cyclin degradation when it was added to frog extracts free of cyclin B , although it formed an active kinase complex with Xenopus cdc 2 . ^^^ A highly purified kinase complex containing a truncated human cyclin A and starfish cdc 2 also failed to switch on the cyclin degradation pathway . ^^^ Whilst free cyclin A had no inhibitory effect , cyclin A cdc 2 kinase delayed degradation of both cyclins A and B induced by cyclin B cdc 2 kinase . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Cyclin A potentiates maturation promoting factor activation in the early Xenopus embryo via inhibition of the tyrosine kinase that phosphorylates cdc 2 . ^^^ Cyclin A was found to form a major complex with cdc 2 , and to bind cdk2 / Eg1 only poorly . ^^^ We conclude that formation of an active cyclin A cdc 2 kinase during early development escapes an inhibitory mechanism that delays formation of an active cyclin B cdc 2 kinase . ^^^ We also found that cyclin B or cyclin A cdc 2 kinases can induce or accelerate conversion of the cyclin B cdc 2 complex from an inactive into an active kinase . ^^^ Cyclin B associated cdc 2 does not undergo detectable phosphorylation on tyrosine in egg extracts containing active cyclin A cdc 2 kinase , even in the presence of vanadate . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The results demonstrate that the treatment of asynchronous cells with 20 Gy ionizing radiation is associated with transient down regulation of the cdc 2 , cyclin A , cyclin B , and cdc 25 genes . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Constitutive expression of B myb cDNA was accompanied by activation of cyclin D 1 and cdc 2 expression but not of cyclin A and cyclin B . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
This G 0 G1 arrest was accompanied by down regulation of the cell cycle regulatory genes cdc 2 , cyclin A , cyclin B , and cdc 25 . ^^^ Results obtained from mRNA half life studies demonstrate that the stability of cdc 2 , cyclin A , cyclin B , and cdc 25 transcripts is similar in control and TPA treated U 937 cells . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
A third H 1 kinase with stable activity at the nonpermissive temperature is comprised of cyclin A and a cdc 2 like 34 kD subunit , which is immunoreactive with anti ' ' PSTAIR ' ' antiserum but is not recognized with antiserum specific for the COOH terminus of p34cdc2 . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Human CDC 2 , cyclin dependent kinase 2 ( CDK 2 ) , cyclin A , and cyclin B 1 were produced in insect cells by infection with recombinant baculoviruses . ^^^ CDC 2 or CDK 2 monomers in lysates of infected cells could be activated by the addition of lysates containing cyclin A or B 1 . ^^^ In contrast , CDC 2 did not bind effectively to cyclin A . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The expression of both CDC 2 and cyclin A was dramatically downregulated upon terminal differentiation of neurons in vivo and in a neuronal precursor cell line , ST15A . p 53 mRNA expression was also downregulated but to a lesser extent ; RB mRNA levels were unchanged during neuronal differentiation . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Addition of 25 nM cyclin A to activated Xenopus egg extracts arrested in the cell cycle by treatment with RNase or emetine activates cdc 2 kinase to the normal metaphase level and stimulates one oscillatory cell cycle . ^^^ High levels of cyclin A cause marked hyperactivation of cdc 2 kinase and a stable arrest at the metaphase point in the cell cycle . ^^^ Kinetic studies demonstrate the concentration of cyclin A added does not affect the 10 min lag period required for kinase activation or the timing of maximal activity , but does control the rate of deactivation of cdc 2 kinase during exit from mitosis . ^^^ These results define a system for investigating the biochemistry and regulation of cdc 2 kinase activation by cyclin A . . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Both cyclin A delta 60 and B delta 97 bind to cdc 2 kinase , keep it hyperactivated and block the completion of mitosis . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The complex of E1A associated proteins included a 33 kDa ATP binding protein , similar in size to a cyclin A associated cdc 2 kinase family member . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The cyclin A polypeptide is associated with a protein that cross reacts with an antibody against the conserved ' PSTAIR ' epitope of p34cdc2 , and the cyclin A cdc 2 complex exhibits protein kinase activity that oscillates with the cell cycle . ^^^ This kinase activity rises more smoothly than that of the cyclin B cdc 2 complexes and reaches a peak earlier in the cell cycle ; indeed , cyclin A is destroyed before nuclear envelope breakdown . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The RT PCR and Western blot analyses have shown that in senescent TIG 1 cells at PDL 64 67 , cdk 2 and cyclin E were selectively repressed at the mRNA and protein levels even after serum stimulation , and cdc 2 and cyclin A were less repressed than cdk 2 and cyclin E . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Cell cycle regulation of the cyclin A , cdc25C and cdc 2 genes is based on a common mechanism of transcriptional repression . ^^^ We now show that CDE mediated repression is also the major principle underlying the periodic transcription of the human cyclin A and cdc 2 genes . ^^^ In addition , the cdc25C , cyclin A and cdc 2 genes share an identical 5 bp region ( ' cell cycle genes homology region ' ; CHR ) starting at an identical position , six nucleotides 3 ' to the CDE . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Although mizoribine did not suppress the expression of CD 25 and cdc 2 kinase , mizoribine markedly suppressed the expression of cyclin A in SA activated B cells . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Genes encoding cdk 1 ( p34cdc2 ) , cyclin A , cyclin B , and the tumor suppressor gene Rb are fundamental regulators of cell cycle progression which associate as a complex with the transcription factor E2F . ^^^ We have investigated levels of mRNA encoding cdk 1 ( p34cdc2 ) , cyclin A , cyclin B , Rb , GAPDH , c myc , and histone H 4 genes for sensitivity to okadaic acid in HeLa cells to determine if transformation altered their regulation . ^^^ When starved cells were subsequently exposed to 19 nM okadaic acid or refed 10 % serum , mRNA levels of cyclin A , cyclin B , cdk 1 , and Rb dramatically increased while mRNA levels for c myc and GAPDH were largely unaffected . ^^^ Despite the cell cycle phase specific block , elevated mRNA levels for cdk 1 , cyclin A , cyclin B , Rb , and suppression of H 4 mRNA levels were detected and persisted for at least 12 hr following okadaic acid removal . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
In addition to the S phase genes , we also find that several genes believed to play regulatory roles in cell cycle progression , such as the cdc 2 , cyclin A , and B myb genes , are also induced by E2F1 . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The expression of certain cell cycle regulatory proteins : cdk 1 , cdk 2 , cdk 4 , cyclin A , cyclin B , cyclin E , Bcl 2 and PCNA was examined in peripheral blood lymphocytes ( PBL ) from 25 cases of chronic lymphocytic leukemias ( CLL ) in order to analyze a possible cell cycle involvement of CLL lymphocytes . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Antibodies raised against human cdk 2 , human cyclin A and mouse cdc 2 kinase showed no detectable effect on the nuclear histone H 1 kinase activity . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
A 17 fold stimulation of GST Cdc 2 kinase activity was obtained by incubation of recombinant human cyclin A with the S . pombe cellular extract prior to affinity purification . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The p 19 fusion protein can associate in vitro with CDK 4 but not with CDK 2 , CDC 2 , or cyclin A , B , E , or D 1 to D 3 . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Multiple species of G 1 cyclins and cyclin dependent kinases are induced sequentially during G 1 phase , and the expression of cyclin A and cdc 2 genes is subsequently induced at the G1 / S boundary . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
While transfer to low serum induced the retinoblastoma protein to enter its dephosphorylated state , and cyclin A , cyclin B and cdc 2 levels to decrease , all as normal , cyclin E , cdk 4 , cdk 2 and the exogenous cyclin D 2 persisted at high levels . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Transcription of histone H 4 , H 3 , and H 1 cell cycle genes : promoter factor HiNF D contains CDC 2 , cyclin A , and an RB related protein . ^^^ Using gel shift immunoassays , we show that CDC 2 , cyclin A , and an RB related protein are ubiquitous subunits of HiNF D binding activity isolated from several cell types . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
These data demonstrate that ets 2 plays a direct role in the regulation of cdc 2 expression and raise the possibility that ets 2 participates in the coordinated regulation of cdc 2 cyclin A expression which is essential for the modulation of cdc 2 regulated processes . . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
These include kinase complexes containing cyclin A , cyclin B , and cyclin D , associated either with CDC 2 , CDK 2 , CDK 4 , or CDK 5 . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The fluctuations in the levels of the proliferation cell nuclear antigen ( PCNA ) , cyclin A , CDC 2 , c myc , and p 53 proteins were monitored in synchronized cultures and in cells undergoing apoptosis by immunofluorescence staining , flow cytometry , and Western immunoblotting . ^^^ When assayed by immunofluorescence staining and flow cytometry , the levels of cyclin A and PCNA increased about 2 fold during the S phase , and the level of CDC 2 was fairly constant during S and slightly decreased during late S / G2 . ^^^ Most importantly , however , the level of staining for c myc , p 53 , cyclin A , CDC 2 , and PCNA increased 50 % 150 % during apoptosis compared to the levels observed in cells at G1 / S . ^^^ Western blot analysis of the steady state levels of PCNA , cyclin A , and CDC 2 revealed an increase in the levels of all three proteins during S , with higher levels of these proteins observed in apoptotic cells compared to the levels observed in cells at G1 / S . ^^^ AGF cells undergoing apoptosis were immunostained for c myc , p 53 , PCNA , cyclin A , and CDC 2 and were viewed by confocal microscopy . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Previously pp60v src , cyclin A , p39mos , and maturation promoting factor ( composed of Cdc 2 and cyclin B ) have been shown to activate mitogen activated protein kinase ( MAPK ) and MAPK kinase ( MEK ) in cell free extracts of Xenopus oocytes . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
AGM 1470 completely abolishes the growth factor induced mRNA expression of cdc 2 and cyclin A and partially inhibits that of cyclin E but has little effect on the mRNA level of cdk 2 , cdk 4 , or cyclin D 1 . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
To elucidate how large T forces the myotubes to traverse each phase of the cell cycle , we examined the expression and activity of Cdk 2 and Cdc 2 , which in complex with cyclin A and cyclin B are essential for S and M phases , respectively in undifferentiated cells . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Surprisingly , many of these genes are expressed in neurons , although cdc 2 , cdk 2 , and cyclin A are not . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Where examined , both Cdc 2 and Cdk 2 , the catalytic subunits known to associate with cyclin A , were activated . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
We investigated whether transfection of cyclin A , alone or in combination with cdc 2 , was sufficient for extension of lifespan or escape from senescence . ^^^ Clones expressing the transfected human cyclin A or cdc 2 genes senesced at a population doubling similar to controls , thereby showing that cyclin A or cdc 2 expression alone was insufficient for escape from senescence . . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Deficiencies that removed one of the Drosophila cdc 2 genes and the cyclin A gene had no effect on replication during embryogenesis . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The expression of cdc 2 , cdk 2 , cyclin B 1 and cyclin A polypeptides was detectable in all lines examined , and moderate variation in protein level does not provide evidence for any obvious abnormalities in the cancer cell lines studied . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
In vitro fusion of endocytic vesicles is inhibited by cyclin A cdc 2 kinase . ^^^ To test for the ability of cyclin A cdc 2 kinase to inhibit endocytic vesicle fusion , we employed recombinant cyclin A proteins . ^^^ By a number of criteria , inhibition of fusion was shown to be due to the action of cyclin A , via the mitosis specific cdc 2 kinase , and not an indirect effect through cyclin B . ^^^ Two stage incubations were used to demonstrate that at least one target of cyclin A cdc 2 kinase is a cytosolic component of the fusion apparatus . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Charged to alanine scanning mutagenesis of Cdc 2 was used previously to localize cyclin A and B and Suc 1 binding sites ( B . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
It has previously been shown that three cell cycle genes , cyclin A , cyclin B and cdc 2 , are not expressed in senescent human fibroblasts . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The cdk 2 gene was induced in late G 1 and cdc 2 and cyclin A genes at the G1 / S boundary in both serum stimulated and dex treated cells . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Concomitantly , expression and associated histone H 1 kinase activity of cdc 2 and cyclin A , two cell cycle regulatory proteins , are repressed by this agent . ^^^ These effects are fully reversible since removal of OA restores cdc 2 and cyclin A expression as well as histone H 1 kinase activity , and the cells resume growth . ^^^ Since cdc 2 and cyclin A have previously been shown to be absolutely required for cell cycle progression it is likely that blockage of synthesis of these components contributes to the cytostatic effects of OA . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
In Xenopus oocytes , mitogen activated protein ( MAP ) kinase can be activated by progesterone treatment or by microinjection of cyclin A , both of which lead to activation of the cdc 2 protein kinase . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Characterization of the in vitro reconstituted cyclin A or B 1 dependent cdk 2 and cdc 2 kinase activities . ^^^ Activation of cdk 2 catalyzed H 1 kinase activity by cyclin A required a 10 min preincubation of the two components , whereas cdc 2 kinase supported phosphate incorporation without a detectable time lag upon the addition of cyclin B 1 , suggesting a slower association rate of cdk 2 with cyclin A compared with cdc 2 and cyclin B 1 . ^^^ Both cdk 2 and cyclin A , as well as cdc 2 and cyclin B 1 , formed stable complexes in the absence of ATP and substrate that could be isolated after glycerol gradient centrifugation . ^^^ Cyclin A activated cdk 2 or cdc 2 phosphorylated p 107 , a pRB related cellular protein , 10 times more effectively than the cyclin B 1 complexed kinases . ^^^ The reconstituted cdc 2 cyclin B 1 complex incorporated 4 5 fold more phosphate into the p 34 subunit of the three subunit ( p 70 , p 34 , and p 14 ) human single stranded DNA binding protein ( also called RP A ) , a DNA replication and DNA repair factor , than cdc 2 cyclin A . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Cyclin A and cdk 2 , but not cyclin B 1 and cdc 2 , were specifically localized at nuclear replication foci , just like the replication protein proliferating cell nuclear antigen . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Sequences within the conserved cyclin box of human cyclin A are sufficient for binding to and activation of cdc 2 kinase . ^^^ Our approach was to map the regions required on the cyclin A molecule for interaction with cdc 2 . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
To determine if the Cdk2 / cyclin A complex could be activated further by phosphorylation of T 160 , complexes were treated with Cdc 2 activating kinase ( CAK ) , purified approximately 12 , 000 fold from Xenopus eggs . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Cyclin A was also associated with cdc 2 , which , contrary to complexes containing cdk 2 , were only activated upon entry into mitosis . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
We found that , like CDC 2 , CDK 2 is also phosphorylated on Thr 14 and Tyr 15 and that treatment of cyclin A or cyclin E immunoprecipitates with bacterially expressed Cdc25M2 ( the mouse homolog of human CDC25B ) increased the histone H 1 kinase activity of these immune complexes 5 to 10 fold . ^^^ Tryptic peptide mapping demonstrated that Cdc25M2 treatment of cyclin A or cyclin B 1 immune complexes resulted in the specific dephosphorylation of Thr 14 and Tyr 15 on CDK 2 or CDC 2 , respectively . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The presence and roles of mitotic cyclins ( cyclin A and cyclin B 1 ) , and cdc 2 and related ( having an N terminal PSTAIRE conserved sequence ) serine / threonine protein kinases were investigated by use of specific antibodies . ^^^ The cyclin A antibodies ( but not the cyclin B 1 antibodies ) and cdc 2 antibodies as well as the PSTAIRE antibodies significantly ( p = 0 . 02 to p < 0 . 001 ) increased ( rather than decreased ) the human sperm penetration rates of zona free hamster ova ; the cyclin A and cdc 2 antibodies showed the strongest enhancing effects . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Immunoaffinity purified Cak 1 phosphorylated monomeric Cdc 2 and Cdk 2 , but not Cdk 4 ; the phosphorylation of both Cdc 2 and Cdk 2 were increased in the presence of recombinant cyclin A . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
In the normal lens , our experiments using in situ hybridization reveal that the expression of cyclin A , cyclin B 1 , cdc 2 and cdk 2 is restricted to the proliferative epithelial cells , with no expression in the differentiating fiber cells . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Cytokine induction of proliferation and expression of CDC 2 and cyclin A in FDC P 1 myeloid hematopoietic progenitor cells : regulation of ubiquitous and cell cycle dependent histone gene transcription factors . ^^^ We therefore focused on factors that are functionally related to activity of the principal cell cycle regulatory element of the histone H 4 promoter : CDC 2 , cyclin A , as well as RB and IRF related proteins . ^^^ Northern blot analysis indicates that cellular levels of cyclin A and CDC 2 mRNAs increase when DNA synthesis and H 4 gene expression are initiated , supporting involvement in cell cycle progression . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The biochemical events that rapamycin has been shown to inhibit are ( a ) activation of p70S6 kinase , ( b ) activation of cdk2 / cyclin E complex , ( c ) phosphorylation of retinoblastoma protein , and ( d ) suppression of cdc 2 and cyclin A transcription . . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Expression and subcellular localization of CDK 2 and cdc 2 kinases and their common partner cyclin A in thyroid epithelial cells : comparison of cyclic AMP dependent and independent cell cycles . ^^^ This convergence included a common induction of the expression of cyclin A and p34cdc2 , and to a lesser extent of p33 / 38cdk2 , which was already expressed in quiescent thyroid cells , and common changes of cdc 2 and CDK 2 phosphorylations as evidenced by electrophoretic mobility shifts . ^^^ These data support the roles of CDK 2 and cdc 2 at G1 / S and G2 / mitosis transitions , respectively . ( 3 ) We were unable to demonstrate in individual cells a strict association between the nuclear appearance of cyclin A and G1 / S transition , and an association of cyclin A and CDK 2 with PCNA stained DNA replication sites . ^^^ On the other hand , the lengthening of G 2 phase in the TSH / cyclic AMP dependent thyroid cell cycle was associated with a stabilization of Tyr 15 inhibitory phosphorylation of cdc 2 and an especially high nuclear concentration of cyclin A and CDK 2 . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Like CDC 2 and CDK 2 , CDK 3 was shown to form a complex with cyclin A in vivo . ^^^ Overexpression of wild type CDC 2 , CDK 2 , CDK 3 , or cyclin A ( but not cyclin B ) markedly elevated the incidence of apoptosis in BCL 2+ cells , which otherwise fail to respond to these agents . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Using the cell line , we investigated the expression of cell cycle associated proteins including cdc 2 , cdk 2 , cyclin A , cyclin Dl , and Rb during entry into or withdrawal from the cell cycle . ^^^ The results indicate that down regulation of neither cdk 2 , cyclin A , cyclin D 1 , nor MAP kinase is necessary to arrest cells in G 0 , but that only Rb dephosphorylation and down regulation of cdc 2 are accompanied by an arrest of cell proliferation in G 0 in the cell line . . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
During this time , cyclin A and cdc 2 levels will fall , preventing the cell from entering mitosis and effectively resetting the clock to G 1 and the ploidy to tetraploid . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The role of cdc 2 , cdc 25 and cyclin A genes in the maintenance of immortalization and growth arrest in a rat embryonic fibroblast conditional cell line . ^^^ The role of the cell cycle control genes cdc 2 , cdc 25 and cyclin A in the maintenance of immortalization and in growth arrest was examined in the tsa 14 , a SV 40 T antigen rat embryonic fibroblast conditional for growth cell line . ^^^ The ability of cdc 2 , or cdc 25 , or cyclin A genes to maintain the tsa 14 immortal phenotype was also examined by electroporations of these genes into the tsa 14 cells . ^^^ Clones over expressing the electroporated cdc 2 , or cdc 25 , or cyclin A , or combinations of these genes growth arrested at the non permissive conditions similar to controls , thereby suggesting that the expression of these genes alone is insufficient for tsa 14 maintenance of immortalization . . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The factor interacting with Site 2 include cdc 2 , cyclin A , an RB related protein and interferon regulatory factors ( IRFs ) . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The E6 / E7 oncoproteins of human papillomavirus ( HPV ) types 16 and 18 are responsible for the efficient immortalization of human genital keratinocytes and we have recently reported that such immortalized cells display alterations in the expression of cyclin A , cyclin B , and cdc 2 . ^^^ To determine whether these alterations were the consequence of E6 / E7 protein expression or whether they resulted from the process of cellular immortalization , we multiply infected primary genital keratinocytes with a retrovirus expressing the HPV 18 E6 / E7 genes and examined the cells for acute , pre immortalization changes in several critical cell growth regulatory proteins including cyclin A , cyclin B , cdc 2 , p 53 and c myc . ^^^ The cell cycle regulating proteins ( cyclin A , cyclin B , cdc 2 and p 53 ) change significantly within days after retroviral infection . ^^^ Increases in the expression of cyclin A , cyclin B and cdc 2 , and decreases in p 53 are therefore among the earliest observable changes in cell regulatory proteins following E6 / E7 gene expression and may be important contributors to the development of cell immortalization . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
We have found that when added to Xenopus cell free extracts free of cyclin B and incapable of protein synthesis , the temporal pattern of cyclin A associated cdc 2 kinase activity showed distinct differences that were dependent on the concentration of cyclin A added . ^^^ When cyclin A was added to a concentration that generated levels of cdc 2 kinase activity capable of inducing nuclear envelope breakdown , the histone H 1 kinase activity profile was bi phasic , consisting of an activation phase followed by an inactivation phase . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Surprisingly , E2F mediated repression is dependent on a contiguous corepressor element that resembles the CHR previously established as a corepressor of the CDE in cell cycle genes derepressed in S / G2 , such as cyclin A , cdc 2 and cdc25C . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
In the early embryo cyclin E 1 complexes exclusively with Cdk 2 and cyclin A is complexed predominantly with Cdc 2 ; only later in development does cyclin A associate with Cdk 2 . ^^^ These results suggest that although in the early Xenopus embryo cyclin E 1 Cdk2 is sufficient to support entry into S phase , cyclin A Cdc 2 provides a significant additional quantity of SPF as its levels rise during S phase . . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
IFN gamma caused a rapid ( within 12 24 h ) down regulation of cyclin A , c myc , and cdc 2 proteins , as well as a disappearance of hyperphosphorylated forms of the retinoblastoma family proteins , Rb and p 130 . ^^^ Several mammary carcinoma cell lines demonstrated resistance to the growth inhibitory effects of IFN gamma and did not exhibit down regulation of cdc 2 and cyclin A expression or a change in hyperphosphorylation of Rb when treated with IFN gamma . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The nonresponders were found to express higher amounts of cellular proliferative markers , such as proliferating cell nuclear antigen ( PCNA ) , cyclin A , and cdc 2 kinase , but lower levels of growth suppressor genes ( TGF beta 1 , TGF beta 2 and p 53 ) before IFN treatment . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
We have previously shown that the promoter complex HiNF D , which interacts with cell cycle control elements in multiple histone genes , contains the key cell cycle factors cyclin A , CDC 2 , and a retinoblastoma ( pRB ) protein related protein . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
We studied several cyclin dependent kinases ( cdk 1 , cdk 2 , cdk 4 , cdk 6 ) and cyclins ( cyclin A , cyclin B 1 , cyclin D 3 and cyclin E ) . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Also , overexpression of cyclin A and cyclin E in SAOS 2 cells enhanced the ability of B MYB , but not c MYB , to transactivate various promoters , including the cdc 2 promoter , the HIV 1 LTR , and the simian virus 40 minimal promoter . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Recent studies have implicated the cell cycle kinase cdc 2 and cyclin A in the inhibition of the fusion of endocytic vesicles in vitro during mitosis . ^^^ Cyclin A dependent kinases , cdc 2 and cdk 2 , were also found in isolated endosome fractions , showing a distinct kinetics of accumulation during the regenerative period . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Isolation of the gene for human CENP A revealed a regulatory motif in the promoter region that directs the late S / G2 expression of other cell cycle dependent transcripts such as cdc 2 , cdc25C , and cyclin A . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
In addition , increased cyclin A and E associated kinase activity was observed , while no change was detected in cyclin B associated kinase activity or in the activation state of cdc 2 kinase . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
In a yeast two hybrid assay , cyclin A 1 can bind to CDK 2 but not to CDC 2 , CDK 4 , and CDK 5 . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
While both cyclin A and cyclin E bind CDK 1 efficiently , only cyclin A activates CDK 1 kinase activity . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
In contrast , 5 Jun transformed fibroblasts continue to cycle and maintain increased levels of retinoblastoma tumor suppressor protein phosphorylation and elevated expression of cell cycle dependent markers such as cyclin A , cyclin dependent protein kinase 2 ( CDK 2 ) , and CDC 2 . 5 Jun transformed fibroblasts nevertheless remain wholly dependent on growth factors for cell multiplication , because cell cycle progression in the absence of serum is accompanied by high rates of apoptotic cell death . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
We have studied the changes in total cyclin A and B 1 levels , cyclin A and B 1 complexed to cdc 2 , cyclin B1cdc2 kinase activity and cyclin D proteins in EL 11 and EL 12 immortalized outgrowth lines . ^^^ No changes in the levels of total cyclin A or cycln A associated to cdc 2 were observed . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Consistent with altered mitotic checkpoint control , cells expressing T displayed elevated protein levels and / or associated activities of the mitotic regulatory proteins cyclin A , cyclin B , Cdc25C and p 34 ( cdc 2 ) . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Second , inhibitory phosphorylation of cdc 2 , the kinase partner of cyclin A , reduced the S phase promoting activity of cyclin A in G 1 . ^^^ CONCLUSIONS : Cyclin A can drive the G1 / S transition , but this function is suppressed by three types of control : cyclin A destruction , inhibitory phosphorylation of cdc 2 , and inhibition by rux . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The elevation of these complexes in arrested 5 mos transformed cells may be the cause of the transcriptional activation of the E2F regulated genes cdc 2 , DHFR , cyclin A , and E2F1 seen in serum starved 5 mos transformed cells . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
To acquire a better understanding of the molecular events leading to quiescence in NR cells , we have analyzed the expression of cdc 2 and of two activators of p 34 ( cdc 2 ) : cyclin A and cyclin B 2 in the developing neuroretina . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Western blot analysis of BIP with cyclins / CDKs and E2F antisera indicated association with cdc 2 , cdk 2 , cdk 4 , cyclin B , cyclin D , cyclin A and E2F 4 but not with cdk 3 , cdk 5 , cdk 6 , E2F 1 , E2F 2 , E2F 3 , E2F 5 and cyclin E . ^^^ Furthermore , we have also demonstrated a direct interaction of in vitro translated BRCA1a and BRCA1b proteins with recombinant cyclin A , cyclin B 1 , cyclin D 1 , cdc 2 , cdk 2 and E2F fusion proteins in vitro . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Here we show , using an inducible p 53 expression system , that wild type p 53 overexpression in EJ bladder carcinoma cells , which have lost functional p 53 , triggers the rapid onset of G 1 and G2 / M growth arrest associated with p 21 up regulation and repression of mitotic cyclins ( cyclin A and B ) and cdc 2 . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Activity of Site 2 is regulated by an E2F independent mechanism involving binding of the oncoprotein IRF 2 and the multisubunit protein HiNF D , which contains the homeodomain CDP / cut , CDC 2 , cyclin A , and the tumor suppressor pRb . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
As the PI increased , the mRNA and protein levels of cyclins D 1 , D 3 and B , the mRNA levels of cyclin A , the protein levels of CDK 4 and CDK 2 , and the activities of CDKs ( CDK 4 , CDK 2 and cdc 2 ) increased in the outer medullae of kidneys after ischemic injury . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The super recovery of cyclin E Cdk 2 coincided with the appearance of a small synchronous population of cells entering into S phase , consistent with transient G 1 phase delay / recovery regulated by cyclin E Cdk 2 , whereas the activities of cyclin A Cdk ' s ( 75 % cyclin A Cdk 2 ; 25 % cyclin A Cdc 2 when inhibition was maximal ) were correlated with rates of total DNA synthesis . ^^^ Western blot analysis demonstrated that levels of Waf1 / p21 did not increase during inhibition of cyclin A Cdk ' s and DNA synthesis in the irradiated p 53 mutated CHO cells ; however , Cdc 2 and Cdk 2 exhibited increased levels of phosphotyrosine . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
UCN 01 prevented the increase of cyclin A / cyclin dependent kinase 2 activity induced by CPT and enhanced Cdc 2 kinase activity . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
However , cyclin E may not simply bypass E2F function in the presence of p 16 , since it restores expression of E2F regulated genes such as cyclin A or CDC 2 . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The cdc25C , cdc 2 and cyclin A promoters are controlled by transcriptional repression through two contiguous protein binding sites , termed the CDE and CHR . ^^^ CDF 1 also binds avidly to the CDE CHR modules of the cdc 2 and cyclin A promoters , but not to the E2F site in the B myb promoter . ^^^ While E2F mediated repression seems to be associated with genes that are up regulated early ( around mid G 1 ) , such as B myb , CDE CHR controlled genes , such as cdc25C , cdc 2 and cyclin A , become derepressed later . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The cdc25C , cyclin A and cdc 2 genes are regulated during the cell cycle through two contiguous repressor binding sites , the CDE and CHR , located in the region of transcription initiation and interacting with a factor termed CDF 1 . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
This decreased proliferative response was accompanied by a reduction in the total fraction of proliferating hepatocytes , as well as by a decline in the induction of cyclin A , cyclin B , and cdc 2 gene expression . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The existence of increased E2F activity during hepatocarcinogenesis was further indicated by the transcriptional induction of putative E2F target genes involved in cell cycle progression , such as endogenous c myc , cyclin A , Cdc 2 , and E2F itself . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The ubiquitous transcription factor , NF Y , plays a pivotal role in the cell cycle regulation of the mammalian cyclin A , cdc25C , and cdc 2 genes , in the S phase activation of the ribonucleotide reductase R 2 gene , in addition to its critical role as a key proximal promoter factor in the transcriptional regulation of the albumin , collagen , lipoprotein lipase , major histocompatibility complex class 2 , and a variety of other eukaryotic and viral genes . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Three sequence specific transcription factors interact with overlapping recognition motifs within Site 2 : interferon regulatory factor IRF 2 ( HiNF M ) , the putative H 4 subtype specific protein H4TF 2 ( HiNF P ) , and HiNF D which represents a complex of the homeodomain protein CDP / cut , CDC 2 , cyclin A and pRB . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
In this study , we isolated cDNAs of starfish cyclin A and Cdc 2 , and monitored extensively the cell cycle dynamics of cyclin A and cyclin B levels and their associated Cdc 2 kinase activity , Tyr phosphorylation of Cdc 2 , and Cdc 25 phosphorylation states throughout meiotic and early embryonic cleavage cycles in vivo . ^^^ Cyclin B , but not cyclin A , associated Cdc 2 was subjected to regulation via Tyr phosphorylation , and phosphorylation states of Cdc 25 correlated with cyclin B / Cdc2 kinase activity with some exceptions . ^^^ Between meiosis 1 and 2 and at the pronucleus stage , cyclin A and B levels remained low , Cdc 2 Tyr phosphorylation was undetectable , and Cdc 25 remained phosphorylated depending on MAP kinase activity , showing a good correlation between these two stages . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Several markers of different phases of the cell cycle , such as cyclin A , cdk 2 , and cdc 2 , fail to be down regulated , indicating the occurrence of cell cycle progression . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The inhibitory effect on DNA synthesis was associated with a delayed progression to S phase of the cell cycle , as determined by flow cytometry and detection of cyclin A and cdc 2 which are two proteins expressed during the S phase . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Moreover , transcription from the cyclin A and cdc 2 promoters becomes impaired when cotransfected with hTAFII 250 containing inactive forms of the N terminal kinase domain . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Using these cell lines , all of which had an intact mitogenic response program to serum , we show that : ( 1 ) an increase in GTP / GDP ratio is an early event that distinguishes cells capable of entering S phase after IGF 1 from cells that do not ; ( 2 ) all cells that are induced to synthesize DNA by IGF 1 have increased phosphorylation of MAP kinases , regardless of their ability to divide ; ( 3 ) the same cell lines display a similar increase in cyclin A and B expression at early times after stimulation ; and ( 4 ) cyclin levels and cyclin B associated cdc 2 kinase activity remain elevated at later times only in cells that undergo cell division . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Surprisingly , mutations in G2 / M phase regulators cyclin A and string ( cdc 25 ) , but not cyclin B 1 , B 3 , or cdc 2 , enhanced the DmcycEJP phenotype without affecting the number of cells entering S phase , but by decreasing the number of cells entering mitosis . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The cdc25C , cyclin A and cdc 2 genes are regulated during the cell cycle through two contiguous repressor binding sites , the CDE and CHR , located in the region of transcription initiation and interacting with a factor termed CDF 1 . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
While the protein levels of cyclin D 1 , cyclin D 3 , CDK 4 , CDK 6 and CDK 2 were still detectable in adult atria , the protein levels of cyclin E , cyclin A , cyclin B , cdc 2 and PCNA were not detectable . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
We also noted in the chCTCF promoter several elements previously characterized in cell cycle regulated genes , including the `` cell cycle dependent element ' ' and `` cell cycle gene homology region ' ' motifs shown to be important for S / G2 specific up regulation of cdc25C , cdc 2 , cyclin A , and Plk ( polo like kinase ) gene promoters . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The presence of cyclin A , associated with cdk 2 or cdc 2 , increased the stability of the interaction between cdk 2 and p13suc1 by a factor of 2 . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Proliferation specific transcription is determined by the integrated activities of three distinct promoter factors interacting with H 4 Site 2 : the interferon regulatory factor IRF 2 ( synonymous with HiNF M ) , HiNF D ( a complex between the homeodomain protein CDP cut and the cell cycle mediators CDC 2 , cyclin A and pRB ) , as well as HiNF P / H4TF 2 . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Cell cycle arrest was independent of the cDNA contained in the expression cassette and was associated with the inappropriate expression and increase in cyclin A , cyclin B 1 , cyclin D , and cyclin dependent kinase p 34 ( cdc 2 ) protein levels . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Regulation by ionizing radiation of CDC 2 , cyclin A , cyclin B , thymidine kinase , topoisomerase IIalpha , and RAD 51 expression in normal human diploid fibroblasts is dependent on p53 / p21Waf1 . ^^^ Among 31 genes analyzed , we observed down regulation in response to IR of the mRNA levels of CDC 2 , cyclin A , cyclin B , thymidine kinase , topoisomerase IIalpha , and RAD 51 . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Protein levels of cdk 4 , cyclin D 1 , cyclin D 2 , cyclin E , p 21 , and p 27 were not affected after CaM inhibition , whereas decreases in the amount of cyclin A and Cdc 2 were observed . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
On human lymphocytes , we measured geometrical features , cell contents in DNA and in cyclin A and CDK 1 proteins , localization and colocalization of these two proteins . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
These biochemical observations indicate that LATS is a novel negative regulator of CDC2 / cyclin A , a finding supported by genetic data in Drosophila demonstrating that lats specifically interacts with cdc 2 and cyclin A . . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Lack of activation of cyclin A and B cdc 2 was associated with a reduced proportion of cells in G2 / M phases . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Many cells , when cultured in suspension , fail to express cyclin A , a regulatory component of cell cycle kinases cdc 2 and cdk 2 and as a consequence , do not enter S phase . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Expression of the cell cycle specific proteins pRb , Rb2 / p130 , p 107 , cyclin A , cdc 2 , and E2F 1 was decreased after DeltaRaf 1 : ER activation in DMS 53 cells . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
ISF treated lymphocytes express normal cyclin A and B 1 levels , but contain reduced levels of cell cycle dependent kinase 1 ( Cdk 1 ) . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
In the present study , we have analysed the effect of the SV 40 large T oncoprotein ( SV LT ) on the function of a different cell cycle regulated transcriptional repressor , CDF , which is the principal regulator of the cdc25C , cyclin A and cdc 2 genes . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Cyclins are required for Cdc 2 kinase activity , and mutations in cyclin A and cyclin B bypass the requirement for mei 41 at the MBT . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
This G2 / M restriction was further characterized by flow cytometry , which revealed cyclin A and cdc 2 kinase accumulation in paclitaxel treated cells . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Prolongation of G2 / M phase was associated with increased levels of cyclin B protein , decreases in cyclin A and cdc 2 proteins , and diminished cdc 2 activity . ^^^ SFV infection was accompanied by large increases in cyclin A and cdc 2 proteins and increased cdc 2 activity . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Whereas no substantial changes in c myc mRNA levels and p 21 , PCNA , cdc 2 , and CDK 2 protein levels were noted in PMEA treated K 562 cells , there was a marked accumulation of cyclin A and , most strikingly , cyclins E and B 1 . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
To examine the expression of specific S phase and M phase cell cycle regulatory proteins and their inhibitors , immunostaining and Western blot analysis was performed for cyclin A , CDK 2 , p 21 and p 27 , cyclin B and cdc 2 . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
A mathematical model for the cell cycle is proposed that incorporates the known biochemical reactions involving both cyclin A and cyclin B , the interactions of these cyclins with cdc 2 and cdk 2 , and the controlling effects of cdc 25 and weel . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
CDK 1 and cyclin A expression is linked to cell proliferation and associated with prognosis in non Hodgkin ' s lymphomas . ^^^ In B NHLs , cdk 1 , cyclin A , as well as cdk 2 , cyclin D 3 and E expression was higher in the aneuploid than in the euploid group . ^^^ CDK 1 and cyclin A showed a significant prognostic value for achievement of complete remission ( Cdk 1 ) and for both disease free ( cyclin A ) and overall survival ( cyclin A and cdk 1 ) : low protein level was associated with the best prognosis in B NHLs . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
We have reported previously that inducible expression of the p 53 gene leads to the cell cycle arrest both at G ( 1 ) and G ( 2 ) / M in association with induction of p 21 and reduction of mitotic cyclins ( cyclin A and B ) and cdc 2 mRNA . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Olomoucine and roscovitine , the potent p 34 ( cdc 2 ) and CDK 2 inhibitors , effectively blocked CD 437 mediated cyclin A and B dependent kinase activation and prevented CD 437 induced cell death . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The disruption of p 21 activity affects the p 21 Rb E2F pathway in that the expression of genes transcriptionally regulated by E2F , such as cyclin A and cdc 2 , were found to be up regulated in injected cells . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
TGF beta 1 induced a transient increase in the expression of Cdc 2 , cyclin A , cyclin B , and cyclin D 1 at an early phase of apoptosis . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Analysis of cell cycle regulatory proteins demonstrated that As ( 2 ) O ( 3 ) ( 2 microM ) did not change the steady state levels of CDK 2 , CDK 4 , CDK 6 , cyclin D 1 , cyclin E and cyclin A , but decreased the protein levels of cdc 2 and cyclin B 1 . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
In uninfected cells the G ( 2 ) / M transition is regulated by cyclin kinase complex containing cdc 2 and , initially , cyclin A , followed by cyclin B . cdc 2 is downregulated through phosphorylation by wee 1 and myt 1 and upregulated by cdc 25C phosphatase . ^^^ The results were as follows . ( 1 ) Cyclin A and B levels were reduced beginning 4 h after infection and were undetectable at 12 to 16 h after infection . ( 2 ) cdc 2 protein also decreased in amount but was detectable at all times after infection . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
In the present work we have characterized an activity present in quiescent cells and absent in cells blocked in S phase , which binds specifically to cyclin A CHR , but not to B myb , or to cdc25C , or to cdc 2 CHRs . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The induction of cyclin A and cdc 2 , the phosphorylation of cdk 2 , the nuclear translocation of cdk 4 and the assembly of cyclin D 3 cdk4 complexes required the synergy of TSH and insulin . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Genetic data show that Rux inhibits the S phase function of the cyclin A ( CycA ) cyclin dependent kinase 1 ( Cdk 1 ) complex ; in addition , it can prevent the mitotic functions of CycA and CycB when overexpressed . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Thus , cardiac failure was induced by ventricular pacing in dogs , and changes in the quantity of cyclin D 2 , cyclin A , cyclin B , cdk 2 , and cell division cycle 2 ( cdc 2 ) in control and paced myocytes were measured . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
We report here the identification of cyclin A and cyclin B proteins and their cofactor ( p 34 ( cdc 2 ) ) in making up the mitosis promoting factor ( MPF ) in protein extracts from egg and muscle tissues of Penaeus vannamei . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Degradation of cyclin A does not require its phosphorylation by CDC 2 and cyclin dependent kinase 2 . ^^^ Here we present evidence from in vitro and in vivo assay systems that the degradation of human cyclin A can be inhibited by kinase inactive mutants of CDK 2 and CDC 2 . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The cell cycle alterations induced by beta lapachone were congruous with changes in cell cycle regulatory proteins such as cyclin A , cyclin B 1 , cdc 2 , and cyclin D 1 . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
In addition , expression levels of cyclin A and cyclin B were reduced during the total course of both TPO and Ha Ras ( G12V ) induced megakaryocytic differentiation , thereby leading to decreased cdc 2 kinase activity . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
We have been able to identify a number of genes , including B myb , cyclin A , cdc 2 , cdc 6 , and DHFR , whose expression is dependent on the presence of E2F3 but not E2F1 . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Specific down regulation of the cyclin A protein level in A549 / p16 1 cells was observed after CPT 11 treatment , whereas cyclin B , cdk 2 , and cdc 2 protein levels were unaffected . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Other E2F regulatory genes , including c myc , cyclin A , and cdc 2 , were also down regulated in mesangial cells cultured on polymerized collagen matrix . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The bipartite repressor elements , termed cell cycle dependent element ( CDE ) / cell cycle regulatory element ( CCRE ) cell cycle homology region ( CHR ) control the growth dependent transcription of the cyclin A , cdc25C , cdc 2 genes . ^^^ Electrophoretic mobility shift assays suggest that two different protein complexes bind independently to the p 130 CDE and CHR elements , and that the protein ( s ) bound to the CDE might be related to those bound on cyclin A and cdc 2 promoters . . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
However , the Rb hSWI / SNF complex persists and is sufficient to maintain repression of the cyclin A and cdc 2 genes , inhibiting exit from S phase . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The present study showed that ALK ( + ) lymphomas , detected with the monoclonal antibody ALKc ( n = 17 ) , had significantly higher average values for the proliferation associated parameters mitotic index , ana / telophase index , growth index ( 10 10 mitotic index apoptotic index , assuming 10 = 3 ) , percentages of Ki 67 ( + ) cells and fraction of cells expressing cyclin A or B or the cell cycle regulatory protein p 34 ( cdc 2 ) than did ALK ( ) ALCLs ( n = 15 ) . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Western blot analysis demonstrated that treatment with As2O3 ( 2 microM ) for 72 h did not change the steady state levels of CDK 2 , CDK 4 , cyclin D 1 , cyclin E , and cyclin B 1 but decreased the levels of CDK 6 , cdc 2 , and cyclin A . ^^^ In addition , As2O3 markedly enhanced the binding of p 21 with CDK 6 , cdc 2 , cyclin E , and cyclin A compared with untreated control cells . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The expression of cyclin dependent kinase 1 ( cdc 2 ) , cyclin A and cyclin B 1 was immunohistochemically studied in 101 hepatocellular carcinomas ( HCC ) . cdc 2 overexpression was directly related to advanced stage , portal invasion , intrahepatic metastasis , poor differentiation , high alpha fetoprotein level , large size , high Ki 67 labeling index and poor prognosis . ^^^ Cyclin A and B 1 overexpression showed similar tendency to that of cdc 2 , but they were not recognized as independent prognostic factors by multivariate analysis . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The behaviour of cell cycle control genes during haematopoietic differentiation was classified into four patterns : ( 1 ) universal upregulation ( cdc 2 , cdk 2 , cyclin A , cyclin B and p 21 ) ; ( 2 ) upregulation in specific lineages ( cyclin D 1 , cyclin D 3 and p 15 ) ; ( 3 ) no induction or stable expression ( cdk 4 , cyclin D 2 , cyclin E and p 27 ) ; and ( 4 ) universal downregulation ( p 16 ) . ^^^ Lineage specific changes included the sustained elevation of cdc 2 and cyclin A during erythroid differentiation , cyclin D 1 and p 15 induction in myeloid lineage and selective upregulation of cyclin D 3 in megakaryocytes . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
These data suggest that the increase in Cdc 2 kinase may play a role in the process from normal liver to chronic hepatitis , whereas the predominant increase in cyclin D 1 , Cdk 4 , cyclin E , cyclin A , and Wee 1 suggests involvement not only in the process from normal liver to chronic hepatitis , but also during transition into HCC . . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
In addition , reduced cyclin D 1 , cyclin A and Cdc 2 levels and increased levels of Cdkn1a ( also known as p 21 ( Waf ) ) were observed in Hsp 25 transfected cells , which probably caused the reduction in cell growth . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Cyclin A interacts with both Cdc 2 and Cdc2c , the Drosophila Cdk 2 homolog , and Roughex inhibits the histone H 1 kinase activities of both cyclin A Cdc 2 and cyclin A Cdc2c complexes in whole cell extracts . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
We had identified earlier a tandem promoter element named cell cycle dependent element ( CDE ) and cell cycle genes homology region ( CHR ) which regulates cell cycle dependent transcription of cdc25C , cyclin A and cdc 2 . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Furthermore , cycloheximide inhibited activation of CDK 2 : cyclin A , which normally precedes CDC 2 kinase activation in caffeine treated cells . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Reflecting the time course of S phase accumulation , cell cycle dependent induction of Cyclin A and Cyclin B was impaired , resulting in reduced activity of Cdk 2 and Cdc 2 kinases . ^^^ IFN alpha treatment inhibits growth of HCC cells by specifically delaying S phase progression , most likely because of inhibition of Cyclin A induction , resulting in decreased activity of the associated Cdk 2 and Cdc 2 kinases . . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Exposure to butyrate increases the expression of the cyclin dependent kinase inhibitors , p21 / Cip1 and p27 / Kip1 , decreases the expression of cyclin A and cyclin B , inhibits the phosphorylation of the retinoblastoma protein ( pRb ) , and decreases the activity of cdk 1 and cdk 2 associated kinases . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Kinase activity was associated with CDC 2 bound to cyclin B 1 , not cyclin A , in both cell lines . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Of the cell cycle control proteins , PCNA , Cdk 2 , and cyclin A were detected at high concentrations ; cdc 2 , Cdk 4 , and cyclin B were detected at very low concentrations ; while cyclin D 1 , cyclin D 3 , Cip 1 , and Kip 1 were not detected . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
This include activation of CDC 2 kinase ( CDK 1 ) which interacts with either cyclin A or cyclin B 1 at the G 1 or G2 / M border , suggeting that this event is one of the major consequences of the drug action on eukaryotic cells . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
CDK 1 and CDK 2 , and cyclin A and cyclin B 1 messenger RNAs were decreased 6 days after castration , increased 6 12 hours after TP treatment , and were expressed at high levels in recurrent CWR 22 tumors . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
E2F1 mediated neuronal apoptosis did not require activation of gene transcription because : ( 1 ) ectopic expression of E2F1 or its mutants lacking the transactivation domain induced neuronal apoptosis , whereas an E2F1 mutant lacking the DNA binding domain did not ; ( 2 ) under all of these conditions , known E2F1 target genes including cyclin A , cdc 2 and p 19 ( ARF ) were not induced ; and ( 3 ) DA evoked neuronal apoptosis was associated with up regulated E2F1 , but not transcription of its target genes . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
These B 19 virus infected cells displayed accumulation of cyclin A , cyclin B 1 , and phosphorylated cdc 2 and were accompanied by an up regulation in the kinase activity of the cdc 2 cyclin B 1 complex , similar to that in cells treated with the mitotic inhibitor . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
There was a correlation between telomerase activity , cell cycle status by BrdU incorporation , and induction of phosphorylated retinoblastoma protein , CDC 2 , CDK 2 , cyclin D 1 , and cyclin A , but not cyclin E and B 1 after 72 hours with multiple ( but not single ) cytokines . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Baicalein mediated G 1 and G 2 growth arrest accompanied by the down regulation of cyclin D 2 , cyclin A , cyclin dependent kinase 1 ( Cdk 1 ) and cyclin dependent kinase 2 ( Cdk 2 ) , and up regulation of p 15 ( Ink4B ) , p 21 ( CIP1 / Waf1 ) , p 53 and cyclin E . ^^^ Evaluation of the kinase activity of cyclin Cdk complexes showed that baicalein decreased Cdk 1 , Cdk 2 , cyclin D 2 and cyclin A expression in endothelial cells , leading to markedly reduced Cdk / cyclin associated kinase activities . ^^^ These results suggest that baicalein inhibits the proliferation of rat heart endothelial cells via G 1 and G 2 arrest in association with the down regulation of the expression and function of Cdk 1 , Cdk 2 , cyclin D 2 and cyclin A proteins , and up regulation of cyclin E , p 15 ( Ink4B ) , p 53 and p 21 ( CIP1 / Waf1 ) . . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
E2F independent activation of histone H 4 gene expression combines contributions of several promoter factors , including HiNF M / IRF2 and the HiNF D / CDP cut complex which contains pRB , CDK 1 , and cyclin A as non DNA binding subunits . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
E 7070 inhibited the phosphorylation of pRb , decreased expressions of cyclin A , B 1 , CDK 2 , and CDC 2 proteins , and suppressed CDK 2 catalytic activity with the induction of p 53 and p 21 proteins in A 549 cells but not in A549 / ER cells . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Earlier studies have shown that cdc 2 kinase is activated during herpes simplex virus 1 infection and that its activity is enhanced late in infection even though the levels of cyclin A and B are decreased below levels of detection . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Phosphorylation of the CCAAT displacement protein ( CDP ) / Cux transcription factor by cyclin A Cdk 1 modulates its DNA binding activity in G ( 2 ) . ^^^ Accordingly , cyclin A Cdk 1 was found to bind to CDP / Cux and modulate its DNA binding activity in vitro and in vivo . ^^^ Interaction with CDP / Cux required the presence of both cyclin A and a cyclin dependent kinase ( Cdk ) activating kinase activated Cdk 1 and involved the Cut homeodomain and a downstream Cy motif . ^^^ In cotransfection studies , cyclin A Cdk 1 inhibited CDP / Cux stable DNA binding and prevented repression of the p 21 ( WAF 1 ) reporter . ^^^ In contrast , mutant CDP / Cux proteins in which serines 1237 and 1270 were replaced with alanines were not affected by cyclin A Cdk 1 . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The G0 / G1 block caused by celecoxib could be attributed to a decreased expression of cyclin A , cyclin B 1 , and cyclin dependent kinase 1 and an increased expression of the cell cycle inhibitory proteins p21Waf1 and p27Kip1 . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The expression of the cyclin dependent kinase inhibitor ( CDKI ) p 27 protein was investigated in relation to ( 1 ) the expression of the cell cycle regulators p 53 , Rb and p 16 and ( 2 ) the proliferation profile as determined by the expression of Ki 67 , cyclin A , and cyclin B 1 in 80 cases of de novo diffuse large B cell lymphomas ( DLBCL ) . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Androgen stimulation also induced the expression of the Cdk 2 and Cdk 1 as well as their regulatory partners , cyclin A and cyclin B , resulting in a corresponding increase in cyclin A / Cdk2 activity in vitro . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Examination of cyclin levels showed increased nuclear cyclin A and cytoplasmic cyclin B and decreased nuclear cdc 2 levels . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Cell cycle analysis revealed that XK 469 is a phase specific cell cycle blocker that is associated with increased levels of cyclin B 1 , cyclin A and p 53 but not CDK 1 ( cdc 2 ) or cyclin E . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Etn and HGF synergistically induced cyclin D ( 1 ) , A and B expression , however , only cyclin B but not cyclin A formed a complex with Cdc 2 . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
CONCLUSION : The cell growth and proliferation of SGC 7901 cell is inhibited by c 9 , t 11 CLA via blocking the cell cycle , with reduced expressions of cyclin A , B ( 1 ) and D ( 1 ) and enhanced expressions of CDKI ( P 16 ( ink4a ) and p 21 ( cip / waf1 ) ) . . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
We previously reported that overexpression of HSP 25 delayed cell growth , increased the level of p 21 ( waf ) , reduced the levels of cyclin D 1 , cyclin A and cdc 2 , and induced radioresistance in L 929 cells . ^^^ Alteration of cell cycle distribution and cell cycle related protein expressions ( cyclin D , cyclin A and cdc 2 ) by hsp 25 overexpression were also recovered by ERK 2 cDNA transfection . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
These include alterations in DNA ploidy , cellular proliferation markers ( PCNA , Ki 67 , Mcm 2 , MIB 1 , MIA , and CSE1L / CAS protein ) , nuclear morphology , the p 53 gene and its related molecule MD M 2 , other cell cycle regulators ( cyclin A , cyclin D , cyclin E , cdc 2 , p 27 , p 73 ) , oncogenes and their receptors ( such as ras , c myc , c fms , HGF , c met , and erb B receptor family members ) , apoptosis related factors ( Fas and FasL ) , as well as telomerase activity . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
METHODS : We immunohistochemically studied three representative G 2 M modulators , cdc 2 , cyclin A and cyclin B 1 in 62 pancreatic adenocarcinomas and 7 cystadenomas . ^^^ RESULTS : Overexpression of cdc 2 , cyclin A and cyclin B 1 , was observed in 54 . 8 , 54 . 9 and 56 . 4 % , respectively , of the pancreatic adenocarcinomas . cdc 2 overexpression was directly related to lymph node metastasis , Ki 67 labeling index ( LI ) , and cyclin A overexpression which was significantly linked to the stage , carcinoma differentiation , tumor size , and lymphatic invasion . ^^^ CONCLUSION : The findings suggest that cdc 2 and cyclin A play a role in the progression of pancreatic adenocarcinoma , while the clinical significance of cyclin B 1 remains to be clarified because of its more random expression . . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Systematic mutagenesis of the DRS enabled a consensus to be derived , and it is notable that this sequence is compatible with cell cycle gene homology region sequences associated with cell cycle dependent elements in the cyclin A , cdc 2 , and CDC25C promoters . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Expression of G 2 M modulators in thyroid neoplasms : correlation of cyclin A , B 1 and cdc 2 with differentiation . ^^^ In this study , we focused on the G 2 M boundary regulators of the cell cycle and investigated the expression of three proteins , cyclin A , cyclin B 1 and cdc 2 . ^^^ Cdc 2 overexpression was also related to carcinoma differentiation ( p < 0 . 0001 ) , and was directly linked to cyclin A overexpression ( p < 0 . 0001 ) , but not to cyclin B 1 overexpression . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Treatment with monensin for 72 h decreased CDK 4 and cyclin A levels in CA 46 cells , and cdc 2 levels in Molt 4 cells . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Based on these results , we speculate that , in the breast cancer cell line T47D , catechin phosphorylated JNK / SAPK and p 38 , and that the phosphorylated JNK / SAPK and p 38 inhibited the phosphorylation of cdc 2 , and regulated the expression of cyclin A , cyclin B 1 , and cdk proteins , thereby causing G 2 arrest . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
We identified 10 suppressors classified into three groups : ( 1 ) gene products regulating Cdk 1 activities , cdk 1 and cyclin A ; ( 2 ) gene products interacting with both microtubules and microfilaments , Actin related protein 87C ; and ( 3 ) gene products interacting with microfilaments , chickadee , diaphanous , Cdc 42 , quail , spaghetti squash , zipper , and scrambled . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The array data enabled us to focus on the role of potential key genes ( e . g . , GADD 153 , CHK 2 , p 21 ( WAF 1 ) , cyclin A , CDK 1 , and DHFR ) that might be targets of MSA in impeding cell cycle progression . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
METHODS : The molecular expressions of Rb , cyclin A , cyclin E , CDK 2 and CDC 2 involved in S phase progression were investigated by westernblot in L 2 and Br 1 3pr 1 cells . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
When we examined the effects of this drug on ACHN cells , monensin decreased the levels of CDK 2 , CDK 6 , cdc 2 , cyclin A and cyclin B 1 proteins . p 21 and p 27 proteins were increased by monensin . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The DNA histogram showed that the cell fraction of the G2 / M phase was increased , and this G2 / M phase arrest was related to a decrease of cdk 1 and cyclin A . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Upregulation of Cdc 2 and cyclin A during apoptosis of endothelial cells induced by cleaved high molecular weight kininogen . ^^^ We report that HKa induced a significant upregulation of Cdc 2 and cyclin A in proliferating endothelial cells , concurrent with a marked increase of Cdc 2 activity . ^^^ The increased expression of Cdc 2 and cyclin A by HKa was not associated with an apparent change in cell cycle profiles of basic fibroblast growth factor stimulated proliferating cells , but closely correlated with a marked increase of apoptosis , suggesting that the elevated Cdc 2 activity is involved in HKa induced apoptosis of proliferating endothelial cells . ^^^ Our results support an emerging hypothesis that Cdc 2 and cyclin A are important regulators for cell cycle as well as for apoptosis . . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Monensin decreased the levels of CDK 2 , CDK 6 , cdc 2 , cyclin A , cyclin B 1 , cyclin D 1 and cyclin E proteins but did not alter CDK 4 protein . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Positive staining for cyclin D 1 , cyclin E , cyclin A , cyclin B 1 , cdk 2 , cdk 4 , cdc 2 , p 53 , p 21 , and p 27 was observed in 63 % , 66 % , 31 % , 32 % , 51 % , 77 % , 71 % , 43 % , 35 % , and 60 % of the 82 carcinomas , respectively . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Finally , forced expression of the NF YA protein in cells committed to differentiate leads to an impairment in the down regulation of cyclin A , cyclin B 1 , and cdk 1 expression and is accompanied by a delay in myogenin expression . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
S phase progression is directed by the cyclin A / CDK2 complex , and the complex of cyclin A with Cdc 2 ( also known as CDK 1 ) is important for G ( 2 ) phase . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Cells did not progress into G 1 but seemed to retract to a G 2 like state containing 4N DNA content , with stabilized cyclin A and cyclin B 1 binding to Thr14 / Tyr15 phosphorylated CDC 2 . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Distinct kinases , cyclin B Cdc 2 , MAPK along with cyclin B and / or cyclin A Cdc 2 and cyclin A Cdc 2 , were unique upstream regulators for Plk 1 activation at meiosis 1 , meiosis 2 and embryonic M phase , respectively , indicating that Plk 1 is not the trigger kinase at meiotic reinitiation . ^^^ We propose that Plk 1 can be activated by either cyclin A or cyclin B Cdc 2 , and its primary target is Myt1 . . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
In this study , we investigated the expression of these modulators , cyclin dependent kinase 1 ( cdc 2 ) , and G 2 cyclins , cyclin A and cyclin B 1 , in primary thyroid lymphoma . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Treatment with STI 571 also led to reduction of cyclin A , B 1 , and CDC 2 levels . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
We find that active RB mediates histone deacetylation on cyclin A , Cdc 2 , topoisomerase IIalpha , and thymidylate synthase promoters . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The S phase block was accompanied by a reduction in the protein levels of cyclin A , cyclin B 1 , cyclin D 1 , cdc 2 , PCNA and the c jun AP 1 component . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The interruption of cell cycle progression in the presence of thialysine was accompanied by a significant decline in the protein level of cdk 4 , cdk 6 , cdc 2 , cyclin A , cyclin B 1 , and cyclin E . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
These nine proteins ( DP 1 , cdc25A , cyclin A , cyclin B ( 1 ) , cyclin D ( 1 ) , cdk 1 , cdk 5 , p 21 ( WAF 1 ) , and GADD 153 ) have been reported previously by our laboratory to be modulated by MSA in human breast and prostate cancer cells . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Cyclin A / Cdk2 complexes regulate activation of Cdk 1 and Cdc 25 phosphatases in human cells . ^^^ We report here that reducing cyclin A expression by RNA interference ( RNAi ) in primary human fibroblasts inhibited activation of Cdc25B and Cdc25C and dephosphorylation of Cdk 1 on tyrosine ( tyr ) 15 . ^^^ These results suggest that cyclin A / Cdk2 complexes are key regulators of Cdc 25 and Cdk 1 activation in human cells . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
At the molecular level , p 53 , cyclin B ( 1 ) , cyclin A and tyrosine ( 15 ) phosphorylated cdk 1 accumulated after HHV 6A infection , indicating an arrest in G ( 2 ) . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Transient transfection assays show that YA aa inhibits transcription from the NF Y target promoters , such as cdc 2 , cyclin A , and cdc25C . ^^^ We constructed a recombinant adenovirus encoding YA aa and found that YA aa expression leads to repression of cell cycle regulatory genes , such as cyclin A , RNR R 2 , DNA polymerase alpha , cdc 2 , cyclin B , and cdc25C . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Measurements of cyclin A and B protein levels , Cdk 2 and Cdc 2 kinase activities , Cdc25C phosphorylation , and Chk 1 kinase activity were consistent with UCN 01 induced abrogation of the S / G2 phase checkpoint in ara C treated cells . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Etodolac induced p21WAF1 / Cip1 and p27Kip1 expression and inhibited CDK 2 , CDK 4 , CDC 2 , cyclin A and cyclin B 1 expression , but did not affect cyclin D 1 or cyclin E . ^^^ In conclusion , p21WAF1 / Cip1 , p27Kip1 , CDK 2 , CDK 4 , CDC 2 , cyclin A , cyclin B 1 and the MAP kinase signaling pathway are involved in growth inhibition and cell cycle arrest by a selective COX 2 inhibitor in HCC cell lines . . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
In contrast , cells stably expressing H179L p 53 arrested in S phase following cisplatin treatment , which correlated with a marked decrease in the expression of cdc 2 , cyclin B 1 and cyclin A , and a decrease in CDK 2 and cyclin A associated kinase activity . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Treatment with rottlerin significantly decreased Cdc 2 activity through the downregulation of cyclin A , cyclin B , and Cdc 2 proteins , whereas the sensitizing effect of rottlerin on TRAIL induced apoptosis was independent of PKCdelta activity . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Overexpression of both viral proteins induces cellular proliferation , retinoblastoma ( pRb ) phosphorylation , and accumulation of products of genes that are negatively regulated by pRb , such as p 16 ( INK4a ) , CDC 2 , E2F 1 , and cyclin A . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Blockade of cell cycle was associated with increased p21 / WAF1 levels and reduced amounts of cyclinB 1 , cyclinA , Cdc 2 , and Cdc25C in a p 53 independent manner . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Resveratrol is able to induce S phase cell arrest and this interference with the cell cycle is associated with an increase of cyclin E and cyclin A , a downregulation of cyclin D 1 , and no alteration in cyclin B 1 and cdk 1 activation . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Low grade superficial papillary tumors induced by activated H ras had no detectable Rb family proteins ( Rb , p 107 , and p 130 ) and late cell cycle cyclins and kinases ( cyclin A , E , and CDK 1 ) , but had increased level of p 16 , p 53 , and MDM 2 . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
By contrast , Smad 7 overexpression interfered with TGF beta 1 mediated attenuation of cyclin A and B levels , inhibition of cdc 2 dephosphorylation and CDK 2 inactivation , up regulation of p 27 , and the maintenance of the retinoblastoma protein ( RB ) in a hypophosphorylated state . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
After performing Cyclin B 1 RNAi , Cyclin B 1 , Cyclin A and Cdk 2 protein levels were found to be markedly downregulated , whereas Cdc 2 was almost unaffected ; S phase fraction increased significantly ; HeLa cell viability and cell colony forming ability were markedly diminished after the RNAi ; Bcl 2 was noticeably attenuated but Bax was hardly changed ; and HeLa cells displayed typical DNA ladder . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
This change is associated with a marked reduction in regulators of S ( cyclin A , cyclin dependent kinase ( cdk ) 2 ) , G2 / M ( cyclin B 1 , cdk 1 , cdc25c ) and G 1 ( cyclin D 1 , cyclin E , cdk 2 , cdk 4 ) phase , and an increase in p 21 and p 27 level . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The RO ET fraction reduced the levels of cyclin A and cell division cycle gene 2 ( cdc 2 ) in premalignant cells and cyclin B 1 , cyclin D 1 , and cdc 2 in the malignant cell lines . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
In flow cytometric analysis , G 2 M cell cycle arrest was specifically observed in GB d 1 cells with MGMT / hMLH1+ and expression of cyclin A and Cdc 2 in GB d 1 cells was significantly reduced by MNU treatment , but not observed in KMG C cells with MGMT+ / hMLH1+ . ^^^ In conclusion , MNU suppressed cell proliferation of MGMT / hMLH1+ gallbladder carcinoma cells by arresting the cell cycle at the G 2 M phase , accompanied by down regulation of cyclin A and Cdc 2 . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Furthermore , the kinase activities of Cdc 2 and Cdk 2 were significantly decreased following sodium butyrate treatment , accompanying downregulation of cyclin A and cyclin B , as well as upregulation of p 21 . ^^^ Forced expression of Cdc 2 plus cyclin B , but not Cdk 2 plus cyclin A , attenuated sodium butyrate / TRAIL induced apoptosis , overriding sodium butyrate mediated downregulation of survivin and XIAP . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Although CDK 2 activity was not directly involved in paclitaxel sensitivity , cyclin A associated kinases did up regulate CDK 1 via phosphorylation . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Drosophila Cks30A interacts with Cdk 1 to target Cyclin A for destruction in the female germline . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
During HD doxorubicin induced apoptosis , the histone H 1 associated activities of Cdc 2 and Cdk 2 both progressively declined in parallel with reductions in cyclin A and cyclin B protein levels . ^^^ In contrast , during LD doxorubicin induced cell death through mitotic catastrophe , the Cdc 2 and Cdk 2 kinases were transiently activated 1 day post treatment , with similar changes seen in the protein levels of cyclin A , cyclin B , and Cdc 2 . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The aim of this study was to elucidate the prognostic values of risk grade and alterations of cell cycle related proteins , including Ki 67 , cyclin A , cyclin B 1 , cyclin D 1 , cyclin E , p 16 , p 21 , p 27 , p 53 , cdc 2 , and cdk 2 , in addition to the conventional factors . ^^^ Overexpression of G 2 M regulators , such as cyclin A , cyclin B 1 , and cdc 2 , was associated with the Ki 67 labeling index ( LI ) ( P = . 0007 , . 0475 , and . 0040 , respectively ) . ^^^ According to univariate analysis , tumor grade ( high risk ) , tumor size ( > or =5 cm ) , mitotic counts ( > or =5 / 50 high power fields ) , Ki 67 LI ( > or =4 . 92 % ) , cyclin A LI ( > or =1 . 61 % ) , and cdc 2 LI ( > or =1 . 25 % ) were all found to be significantly associated with a shorter period of disease free survival ( P = . 0001 , . 0270 , . 0004 , . 0001 , . 0001 , and . 0011 , respectively ) . ^^^ Furthermore , alteration of G 2 M regulators , such as cyclin A , cyclin B 1 , and cdc 2 , is also a useful marker for predicting aggressive behavior and play an important role , at least in part , in the cell proliferation of GIST . . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Cks30A interacts with Cdk 1 to target cyclin A for destruction in the female germline , possibly through the activation of a novel germline specific CDC 20 protein , Cortex . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The latter cells were the most responsive to betulinic acid , showing a selective decline in the cdk 4 protein , without a comparable change in other key cell cycle proteins like cdc 2 , cdk 2 , cdk 7 or cyclin A , prior to apoptosis associated PARP fragmentation . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The expression of cyclin A , cyclin B , cyclin D 1 , cdk 2 , cdk 4 , cdc 2 , proliferating cell nuclear antigen ( PCNA ) , p 21 ( Cip / WAF1 ) , and p 27 was examined with immunoblotting analysis . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The expression of cyclin A , cyclin B 1 and cyclin dependent kinase 1 , the regulators required for the progression from G 2 to mitosis , were all decreased by these fatty acids ( western blot ) . ^^^ Since omega 3 fatty acids had no effect on the S phase , thus ruling out an involvement of cyclin A in their anti proliferative effect , we examined whether the regulation of the cyclin dependent kinase 1 cyclin B 1 complex was altered . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The G 2 M arrest by silibinin and silymarin was associated with decreased levels of cyclin B 1 , cyclin A , pCdc 2 ( Tyr 15 ) , Cdc 2 , and an inhibition of Cdc 2 kinase activity . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The expression levels of cyclin dependent kinase 2 and cyclin A , as well as cyclin dependent kinase 1 and cyclin B , were decreased by TH , and after withdrawal not only did these regulators of Rb phosphorylation and mitosis increase in their expression but so too did the D 1 and D 3 cyclins . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Analysis of the arrays showed that expression of several genes such as clusterin , secreted frizzled related protein mRNA ( sFRP 1 ) , CREB binding protein ( CBP ) , and others was higher in the apoptotic cells whereas expression of certain genes including survivin , cell division cycle 2 homolog A ( CDC 2 ) , and cyclin A was lower . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NF Y transcription factor binds to CCAAT boxes on promoters of cell cycle regulatory genes such as cdc 2 , cyclin B , cdc25C , and cyclin A . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Immunoblot assay showed that chalcone significantly decreased the expression of cyclin B 1 , cyclin A and Cdc 2 protein , as well as increased the expression of p 21 and p 27 in a p 53 independent manner , contributing to cell cycle arrest . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Analysis of the cell cycle regulatory proteins demonstrated that squamocin did not change the steady state levels of Cdk 2 , Cdk 4 , cyclin A , cyclin B 1 , cyclin D 3 and cyclin E , but decreased the protein levels of Cdk 1 and Cdc25C . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
In this study , under treatment with various concentrations of PA in MDA MB 231 cell line , we checked mRNA levels for cyclin A and cyclin B 1 and the protein levels of cyclin A and cyclin B 1 , Cdc 2 ( cyclin dependent kinases ) , p 21 ( waf1 / cip1 ) and P 27 ( Kip 1 ) ( cyclin dependent kinase inhibitors ) , Cdc25C , Chk 2 and Wee 1 kinase ( cyclin dependent kinase relative factors ) in cell cycle G2 / M phase . ^^^ From those results , we determined that PA arrests MDA MB 231 cells at the G2 / M phase by ( 1 ) inhibiting synthesis or stability of mRNA and their downstream protein levels of cyclin A and cyclin B 1 , ( 2 ) increasing p 21 ( waf1 / cip1 ) and P 27 ( kip 1 ) levels , ( 3 ) increasing Chk 2 , thus causing an increase in Cdc25C phosphorylation / inactivation and inducing a decrease in Cdc 2 levels and an increase in Wee 1 level . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
On the other hand , TIS 21 inhibits degradations of cyclin A and cyclin B 1 at G2 / M phase , and directly binds to Cdc 2 , resulting in the failure of mitotic exit and then increasing the tumor cell death , when stimulated by high concentration of EGF . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The results revealed that HY 52 was found to inhibit the proliferation of HeLa cells through inducing a G 1 phase arrest by inhibiting pRb phosphorylation via an up regulation of p21WAF1 / CIP1 and p27KIP1 , and G2 / M phase arrest by down regulation of CDC 2 , cyclin A , and cyclin B1 . . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The sodium butyrate induce cell cycle arrest was associated with the decrease of Cdc 2 , Cdk 4 and cyclin A in BCBL 1 cells without altering the protein levels of p 21 ( CIP1 / WAF1 ) . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Levels of p 130 rose after drug treatment , and it was the primary RB family member recruited to the S phase promoters cyclin A and PCNA and G 2 M promoters cyclin B and cdc 2 , remaining present for the entire 8 day time period . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
This effect was associated with a reduction in the expression of regulatory proteins of G ( 2 ) M ( cyclin dependent kinase 1 and cdc25c ) and S ( cyclin A ) phases , and with an increase in the expression of apoptosis related proteins , such as p 53 and p 21 . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Expression of CDK 2 , CDC 2 and cyclin A was induced rapidly in the process of radiation induced apoptosis . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Dual regulation of the anaphase promoting complex in human cells by cyclin A Cdk 2 and cyclin A Cdk 1 complexes . ^^^ Using Plk 1 protein level as readout of APC activity , we show that APC is inactivated during S phase in human cells by both cyclin A Cdk 2 and cyclin A Cdk 1 complexes . ^^^ Although Cdk 2 is the dominant partner of cyclin A in these settings , cyclin A also binds Cdk 1 . ^^^ These results provide further evidence that cyclin A Cdk 2 and Cdk 1 complexes display overlapping and partially redundant roles in preparing cells for mitosis , through regulation of the APC . . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
However , other G2 / M regulatory molecules , such as cyclin A , Cdc 2 , Cdk 2 , Wee 1 and Cdc25C , were not modulated by curcumin treatment . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
When either Cdk 2 or Cdk 1 is ablated using RNAi , there were complex shifts of cyclin A towards its reciprocal partner , i . e . , when Cdk 2 is ablated , cyclin A redistributes to Cdk 1 ; when Cdk 1 is ablated , cyclin A forms more abundant complexes with Cdk 2 . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Furthermore , p 21 ( Cip1 / Waf1 ) was enhanced , and cyclin A , B 1 and cdk 1 decreased . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Furthermore , HS doses of 500 ng / ml also downregulated retinoblastoma , Cyclin A and CDK 1 protein expression , indicating that high doses of osteoblast HS negatively regulate cell cycle , resulting in growth arrest ; when high doses of HS were withdrawn after a prolonged period , linear cell growth was reestablished . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
In the absence of Cyclin A , premature Fizzy related / Cdh1 activity results in the premature degradation of the Cdk 1 activators Cyclin B and Cyclin B 3 , and apparently of String / Cdc25 phosphatase as well . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The expression of cyclin A , cyclin B 1 , cdk 1 , and cdc25C , as well as the cdk 1 associated kinase activities , is upregulated after DNA damage , provoking a mutant p53 / NF Y dependent increase in DNA synthesis . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Cyclin A 2 activates cdc 2 ( Cdk 1 ) on passage into mitosis and Cdk 2 at the G1 / S transition . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
To begin to examine the function of the A type cyclins during meiosis in the male , we have examined the developmental and cellular distribution of the cyclin A 1 and cyclin A 2 proteins , as well as their candidate cyclin dependent kinase partners , Cdk 1 and Cdk 2 , in the spermatogenic lineage . ^^^ In the testis , cyclin A 1 has been shown to bind both Cdk 1 and Cdk 2 but we show here that cyclin A 2 binds only Cdk 2 . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
We found that the concentrations of cyclin A 2 and cyclin B 1 ( at their peak levels in the G ( 2 ) phase ) were about 30 fold less than that of their partner CDC 2 . ^^^ All the cyclin A 2 and cyclin E 1 are in complexes with CDC 2 and CDK 2 , but there are some indications that a significant portion of cyclin B 1 may not be in complex with CDC 2 . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Membrane anchored cyclin A 2 triggers Cdc 2 activation in Xenopus oocyte . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
This was associated with the earlier expression of the cell cycle promoting cyclin A 2 , cyclin E , cyclin B 1 , cyclin F , and cyclin dependent kinase 1 ( Cdk 1 ) genes and diminished protein levels of Cdk inhibitor p21Cip1 . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
The upper band of cyclin A 2 was first detected at 2 hr after the treatment , corresponding to the timing of Cdc 2 kinase activation . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Elevated levels of cyclin A 2 , Cdk 1 and Cdk 2 were detected in the majority of GCTs and were correlated with the invasiveness of the tumors ( p < 0 . 05 ) . ^^^ Cyclin A 2 expression was strongly correlated with that of its catalytic partners Cdk 1 and Cdk 2 in all types of testicular tumors examined ( p < 0 . 05 ) , whereas a strong correlation between cyclin A 1 and Cdk 1 or Cdk 2 was only seen in non seminomatous GCTs ( p < 0 . 05 ) . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Immortalization in a normal foreskin fibroblast culture following transduction of cyclin A 2 or cdk 1 genes in retroviral vectors . ^^^ Here we report the immortalization of an HDF cell line following transduction with cyclin A 2 or cdk 1 human genes via retroviral vectors . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Our present study focused on the expression and prognostic significance of cyclin A 1 and a panel of cell cycle regulatory proteins including cyclin A 2 , cyclin B 1 , cyclin E , CDK 1 , CDK 2 , p 21 and p 27 in bone marrow samples from 40 patients with AML . ^^^ However , expression of cyclin A 2 , cyclin B 1 , cyclin E , CDK 1 , CDK 2 , p 21 and p 27 did not show prognostic significance in these AML patients . . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
We observed elevated levels of S phase and mitosis in tissues of p 27 ( / ) Cdk 2 ( / ) mice concomitantly with elevated Cdc 2 activity in p 27 ( / ) Cdk 2 ( / ) extracts . p 27 binds to Cdc 2 , cyclin B 1 , cyclin A 2 , or suc 1 complexes in wild type and Cdk 2 ( / ) extracts . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
We observed a gradual decrease of Retinoblastoma protein ( Rb ) phosphorylation and reduced expression of E2F target genes , like Cdc 2 and cyclin A 2 , during embryogenesis and in embryonic fibroblasts ( MEFs ) . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Leukemias with a 5 / del ( 5q ) have a higher expression of genes involved in cell cycle control ( CCNA 2 , CCNE 2 , CDC 2 ) , checkpoints ( BUB 1 ) , or growth ( MYC ) , and loss of expression of the gene encoding IFN consensus sequence binding protein ( ICSBP ) . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
Leukemias with a 5 / del ( 5q ) have a higher expression of genes involved in cell cycle control ( CCNA 2 , CCNE 2 , CDC 2 ) , checkpoints ( BUB 1 ) , or growth ( MYC ) , and loss of expression of the gene encoding interferon consensus sequence binding protein ( ICSBP ) . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
This analysis predicts that cancer cells manifesting a stem cell like expression profile of a death from cancer signature would exhibit the following features : a concomitantly increased expression of certain members of inhibitor of apoptosis protein ( IAP ) family ( Survivin and XIAP ) ; activation of mitotic spindle check point proteins ( BUB 1 , BUB 3 , KNTC 2 , Mad 2 , PLK 1 , PLK 4 , STK6 / Aurora A ) ; and elevated levels of certain cell cycle control / marker proteins ( CCNB 1 , CCNB 2 , CCND 1 , CCNA 2 , CDC 2 , CDC 25 , Ki 67 , USP 22 ) . ^^^
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA
Interacting proteins: P20248 and P06493	Pubmed	SVM Score :0.0
NA