| Interacting proteins: P16949 and P06493 |
Pubmed |
SVM Score :0.0 |
| The phenotype of a `` Cdc 2 kinase target site deficient ' ' mutant of oncoprotein 18 reveals a role of this protein in cell cycle control . ^^^ We have investigated the potential role of the Cdc 2 kinase mediated phosphorylation of these two sites by expressing a `` Cdc 2 kinase target site deficient ' ' mutant of Op 18 ( Op 18 S25 , 38A ) , and analyzed the phenotype on the level of cell cycle regulation . ^^^ |
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| Interacting proteins: P16949 and P06493 |
Pubmed |
SVM Score :0.0 |
| Our previous site mapping studies of Op 18 also revealed that basal phosphorylation of Op 18 is mainly located on Ser 38 , which was found to be the primary in vitro phosphorylation site of p13suc1 precipitated cdc 2 kinase activities . ^^^ These findings raised the possibility that Op 18 may be a substrate for both receptor regulated calcium induced protein kinases and the MAP kinase family , as well as being a substrate for the cell cycle regulated cdc 2 kinase family . ^^^ In vitro phosphorylation experiments , employing two distinct members of the cdc 2 kinase family , were consistent with involvement of both p 34 cdc2 and p 33 cdk2 in cell cycle regulated phosphorylation of Ser 25 and Ser 38 of Op 18 . ^^^ These findings suggest that Op 18 may be a physiological substrate for several members of the cdc 2 kinase family during both the S phase and the mitotic phase of the cell cycle . . ^^^ |
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| Interacting proteins: P16949 and P06493 |
Pubmed |
SVM Score :0.0 |
| The present study shows that the MAP kinase has a 20 fold preference for Ser 25 as opposed to Ser 38 of Op 18 , while cdc 2 kinases have a 5 fold preference for the Ser 38 residue . ^^^ |
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| Interacting proteins: P16949 and P06493 |
Pubmed |
SVM Score :0.0 |
| During mitosis , phosphorylation of Op 18 by cdc 2 is necessary but not sufficient for Op 18 inhibition . ^^^ |
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| Interacting proteins: P16949 and P06493 |
Pubmed |
SVM Score :0.0 |
| Following extracellular stimuli , stathmin is phosphorylated on four serines ( Ser 16 , Ser 25 , Ser 38 , and Ser 63 ) by several kinases , such as mitogen activated protein ( MAP ) , cdc 2 kinase , protein kinase A , and Ca2+ / calmodulin dependent kinase Gr . ^^^ |
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| Interacting proteins: P16949 and P06493 |
Pubmed |
SVM Score :0.0 |
| Five different protein kinases , protein kinase A , MAP kinase , cdc 2 kinase , glycogen synthase kinase 3 and casein kinase 2 , were used to modify stathmin , since it is known that these kinases could phosphorylate several residues that are modified in vivo and could have important roles in stathmin function . ^^^ This activity is higher than that of stathmin phosphorylated by protein kinase A , MAP kinase or cdc 2 kinase , whereas phosphorylation of the protein with casein kinase 2 or glycogen synthase kinase 3 resulted in a slight increase of the depolymerizing activity . . ^^^ |
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| Interacting proteins: P16949 and P06493 |
Pubmed |
SVM Score :0.0 |
| Although the role of phosphorylation of stathmin by p 34 ( cdc 2 ) kinase in the assembly of the mitotic spindle is well established , the role of dephosphorylation of stathmin in mitosis is unknown . ^^^ |
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| Interacting proteins: P16949 and P06493 |
Pubmed |
SVM Score :0.0 |
| These results strongly suggest that heat stress activates Cdk 1 which phosphorylates Ser 37 on the stathmin molecule . ^^^ |
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| Interacting proteins: P16949 and P06493 |
Pubmed |
SVM Score :0.0 |
| We propose that the active cyclin A cdc 2 kinase generated without a lag phase from neo synthesized cyclin A and cdc 2 may cause a rapid switch in the equilibrium of cyclin B cdc 2 complexes to the tyrosine dephosphorylated and active form of cdc 2 during early development , owing to strong inhibition of the cdc 2 specific tyrosine kinase ( s ) . ^^^ |
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| Interacting proteins: P16949 and P06493 |
Pubmed |
SVM Score :0.0 |
| Phorbol 12 , 13 dibutyrate and 1 , 2 dioctanoylglycerol , activators of protein kinase C ( PKC ) that stimulate DNA synthesis in serum deprived Swiss 3T3 fibroblasts , induce histone H 1 kinase activity associated with anti cdc 2 immunoprecipitates after a lag period of 15h , a time point close to G1 / S boundary of the cell cycle in these cells . ^^^ |
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| Interacting proteins: P16949 and P06493 |
Pubmed |
SVM Score :0.0 |
| Kinetic studies demonstrate the concentration of cyclin A added does not affect the 10 min lag period required for kinase activation or the timing of maximal activity , but does control the rate of deactivation of cdc 2 kinase during exit from mitosis . ^^^ |
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| Interacting proteins: P16949 and P06493 |
Pubmed |
SVM Score :0.0 |
| Incubation of the particulate fraction from interphase cells with the protein kinase cdc 2 activates it for cyclin ubiquitin ligation , after a lag of about 30 min . ^^^ |
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| Interacting proteins: P16949 and P06493 |
Pubmed |
SVM Score :0.0 |
| Activation of cdk 2 catalyzed H 1 kinase activity by cyclin A required a 10 min preincubation of the two components , whereas cdc 2 kinase supported phosphate incorporation without a detectable time lag upon the addition of cyclin B 1 , suggesting a slower association rate of cdk 2 with cyclin A compared with cdc 2 and cyclin B 1 . ^^^ |
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| Interacting proteins: P16949 and P06493 |
Pubmed |
SVM Score :0.0 |
| Inhibition of phosphatase 2A , the major okadaic acid sensitive Ser / Thr phosphatase , in these extracts , provokes Cdc 2 kinase amplification and concomitant hyperphosphorylation of Cdc 25 phosphatase , with a lag of about 1 h . ^^^ |
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| Interacting proteins: P16949 and P06493 |
Pubmed |
SVM Score :0.0 |
| The observed lag corresponds to a delay in the G ( 1 ) phase activation of the cyclin dependent kinase NIMX ( cdc 2 ) . ^^^ Disruption of cmkC is not lethal , but spores lacking CMKC also germinate with delayed kinetics and a lag in the activation of NIMX ( cdc 2 ) . ^^^ |
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| Interacting proteins: P16949 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P16949 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P16949 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16949 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16949 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16949 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16949 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16949 and P06493 |
Pubmed |
SVM Score :0.0 |
| NA |
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