| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| Selective induction of cell cycle regulatory genes cdk 1 ( p34cdc2 ) , cyclins A / B , and the tumor suppressor gene Rb in transformed cells by okadaic acid . ^^^ Genes encoding cdk 1 ( p34cdc2 ) , cyclin A , cyclin B , and the tumor suppressor gene Rb are fundamental regulators of cell cycle progression which associate as a complex with the transcription factor E2F . ^^^ We have investigated levels of mRNA encoding cdk 1 ( p34cdc2 ) , cyclin A , cyclin B , Rb , GAPDH , c myc , and histone H 4 genes for sensitivity to okadaic acid in HeLa cells to determine if transformation altered their regulation . ^^^ When starved cells were subsequently exposed to 19 nM okadaic acid or refed 10 % serum , mRNA levels of cyclin A , cyclin B , cdk 1 , and Rb dramatically increased while mRNA levels for c myc and GAPDH were largely unaffected . ^^^ Despite the cell cycle phase specific block , elevated mRNA levels for cdk 1 , cyclin A , cyclin B , Rb , and suppression of H 4 mRNA levels were detected and persisted for at least 12 hr following okadaic acid removal . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| In contrast to previously characterized cyclin dependent and Rb associated kinases , such as cdk 1 ( cdc 2 ) and cdk 2 , G2 / M RbK 1 ) is not depleted by incubation with p13suc beads , 2 ) is not detected with antisera against several Rb associated cyclins cdks , and 3 ) associated with Rb via the Rb amino terminus , a region that is dispensable for interaction with other Rb associated kinases . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| Protein levels of cell cycle mediators , cyclin dependent kinases ( CDK 1 , 2 , 4 , 5 ) , cyclin proteins ( A , B , D 1 D3 and E ) , proliferating cell nuclear antigen ( PCNA ) , tumor suppressor proteins ( p 53 and Rb ) , and CDK inhibitory proteins ( p16Ink4 , p21Waf1 and p27Kip1 ) were examined in F 344 rats following 70 % partial hepatectomy or a single dose of WY 14 , 643 over 96 and 48 h time courses , respectively . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| In addition , CDK 1 and CDK 2 kinase activities were upregulated by androgen in parallel with hyperphosphorylation of retinoblastoma ( Rb ) protein . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| In addition , senescence is associated with increased binding of the cyclin dependent kinase inhibitor ( CDK 1 ) p 16 ( INK4a ) to CDK 4 , down regulation of cyclin E protein levels ( and consequent loss of cyclin E / CDK2 activity ) , underphosphorylation of the retinoblastoma protein RB and subsequent increased levels of E2F4 RB repressive complexes . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| Steady state levels of Cyclin B 1 and its associated kinase Cdk 1 were suppressed in a RhoB dependent manner in cells fated to undergo FTI induced apoptosis . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| The response of tumors to MET stress depends on their mutational status , however , it always involves inhibition of CDK 1 and in most cases the upregulation of p 21 , p 27 , GADDs and 14 3 3sigma in response to upregulation of TGF beta , IRF 1 , TNF alpha , Rb and / or MDA 7 and the downregulation of PI3K , RAS and NF kappaB . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| The expression levels of cyclin dependent kinase 2 and cyclin A , as well as cyclin dependent kinase 1 and cyclin B , were decreased by TH , and after withdrawal not only did these regulators of Rb phosphorylation and mitosis increase in their expression but so too did the D 1 and D 3 cyclins . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| The possibility that local diffusible factors play a major role in determining tissue specific effects of estrogen was examined by transplanting uterine tissues into the brain , muscle and kidney of adult rats and then comparing the effects of estrogen on the incorporation of [ 3H ] thymidine and the expression of Fos , cdc 2 and Rb like immunoreactivity ( IR ) on native and transplanted uterine tissues , as well as in estrogen concentrating regions of the brain adjacent to the uterine grafts . ^^^ In native uteri , estrogen treatment stimulated Fos , cdc 2 , and Rb like IR , as well as [ 3H ] thymidine incorporation , within lumenal and glandular epithelial cells . ^^^ When uterine tissues were transplanted to brain , Fos , cdc 2 , and Rb like IR epithelial cells , as well as many [ 3H ] thymidine incorporating uterine epithelial cells , were observed in all estrogen treated animals and in some non estrogen treated animals as well . ^^^ In contrast , estrogen did not stimulate cell division and did not induce Fos , cdc 2 , or Rb like IR within estrogen concentrating neuronal regions of the ventromedial hypothalamus . ^^^ In addition , the data provide the first evidence for estrogen regulation of cdc 2 and Rb expression in normal uterus . . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| The product of the retinoblastoma susceptibility gene ( Rb ) is a substrate of the cell cycle regulated cdc 2 and cdk kinases . ^^^ In in vivo phosphorylated Rb protein , we have found ten phosphotryptic peptides , all of which could be phosphorylated by cdc 2 kinase , p34cdc2 , in vitro . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| Low grade superficial papillary tumors induced by activated H ras had no detectable Rb family proteins ( Rb , p 107 , and p 130 ) and late cell cycle cyclins and kinases ( cyclin A , E , and CDK 1 ) , but had increased level of p 16 , p 53 , and MDM 2 . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| Moreover , substantial evidence that H / RS cells have defective cell cycle and apoptosis regulation has been provided by studies showing that these cells are characterized , in a large proportion of cases , by alterations of the p 53 , Rb and p 27 tumor suppressor pathways , overexpression of cyclins involved in the G1 / S and G2 / M transition such as cyclins E , D 2 , D 3 , A and B 1 , overexpression of cyclin dependent kinases such as CDK 1 , 2 and 6 and overexpression of anti apoptotic proteins such as c FLIP , bcl xl , c IAP 2 , 10 linked I4P and survivin . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| However , at least four regulatory and structural proteins ( Cdk 1 , cyclin B 1 , PML and lamin B 1 ) differ in their presence in RB and RF . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| The identities of the Rb kinases are unknown , but recent evidence suggests that the cdc 2 gene product could participate in Rb phosphorylation . ^^^ The suppression of Rb phosphorylation is suggested to be mediated through inhibition of cdc 2 kinase activity by TGF beta . . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| The retinoblastoma susceptibility gene product ( Rb ) specifically represses cdc 2 transcription in cycling cells via 136 bp of 5 ' flanking sequence located between 245 and 109 within the UNC region . ^^^ A model is proposed where Rb negatively regulates the cdc 2 promoter in non cycling and cycling G 1 cells . . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| In studies on the kinase that phosphorylates the RB proteins , we have previously found that RB proteins can be phosphorylated by purified cdc 2 kinase . ^^^ In this study , we noted that RB proteins immunoprecipitated from human cell lysates are weakly phosphorylated in the absence of purified cdc 2 kinase . ^^^ Furthermore , RB proteins were shown to be phosphorylated in nuclear extracts by some endogenous cdc 2 like kinase ( s ) . ^^^ These results suggest that cdc 2 like kinase is the main kinase for phosphorylation of RB proteins in vivo . . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| The expression of both CDC 2 and cyclin A was dramatically downregulated upon terminal differentiation of neurons in vivo and in a neuronal precursor cell line , ST15A . p 53 mRNA expression was also downregulated but to a lesser extent ; RB mRNA levels were unchanged during neuronal differentiation . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| These studies link regulation of cdc 2 level , RB phosphorylation state , and induction of differentiation by RA and suggest that alterations in the cdc 2 gene or in genes controlling its regulation contribute to tumorigenesis . . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| Of several protein kinases tested , only cdc 2 kinase phosphorylates RB efficiently and all 10 peptides can be phosphorylated by cdc 2 in vitro . ^^^ Removal of cdc 2 from mitotic cell extracts by immunoprecipitation causes a concomitant depletion of RB kinase activity . ^^^ These results indicate that cdc 2 or a kinase with similar substrate specificity is involved in the cell cycle dependent phosphorylation of the RB protein . . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| These results suggest the possibility that the function of RB protein is regulated by CDC 2 kinase . . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| These results suggest the possibility that the function of RB protein is regulated by CDC 2 kinase . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| Expression of Rb , E2F1 , cdc 2 , and D , and B cyclins in developing spinal cord . ^^^ The spatial and temporal distribution of transcripts for the tumor suppressor gene Rb , transcription factor E2F1 , cdc 2 kinase , cyclins D 1 , D 2 , B 1 and B 2 during neurogenesis of the spinal cord was determined by in situ hybridization . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| The delayed G 0 > S progression in fos expressing cells was accompanied by the inappropriate levels or kinetics of expression of several cell cycle regulated genes ( cyclin D 1 , cdc 2 , cdk 2 , cdk 4 and rb ) . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| Cells en route to apoptosis are characterized by the upregulation of c fos , c myc , c jun , cdc 2 , and RB phosphorylation , resembling events of early cell cycle traverse . ^^^ Investigating the regulation of early cell cycle genes during this process , we found that c myc , c jun , c fos , and cdc 2 protein presence is induced after serum deprivation , when the phosphorylated form of the RB protein also appears . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| BON cells also exhibited increased expression of c myc and cdc 2 kinase mRNA and protein ; TGF beta 1 , p 53 and retinoblastoma ( RB ) mRNA and protein levels were decreased . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| AGM 1470 inhibits growth factor induced activation of candidate RB kinases cdc 2 and cdk 2 but fails to inhibit them directly in vitro . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| We also found that apoptotic mouse 3T3 fibroblasts express c fos , c myc , c jun , and cdc 2 , as well as the upregulation of RB phosphorylation and BrdU incorporation , just before final DNA fragmentation and death . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| The state of phosphorylation of retinoblastoma ( RB ) protein is regulated by CDK 2 and CDC 2 kinases . ^^^ We observed that the IFN alpha enhances the expression of underphosphorylated RB protein in Daudi cells released from the G1 / S , and this was closely associated with the inhibition of CDK 2 kinase and not CDC 2 kinase activity . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| The `` low risk ' ' human papillomavirus types ( HPV 6 or 11 ) were determined by in situ hybridization and PCR amplification followed by direct sequencing using consensus primers from the highly conserved L 1 region in six different condylomas . mRNA levels of certain cytokines ( e . g . , TGF beta 1 , IFN beta ) , tumor suppressor genes ( RB , p 53 ) , c myc , epidermal growth factor receptor , and cdc 2 kinase were measured by RT / PCR . ^^^ Western blot experiments demonstrated a higher proportion of the hyperphosphorylated form of RB protein and a higher level of cdc 2 kinase and c myc , but low p 53 and TGF beta 1 levels in condylomas . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| The cell cycle dependent , PKC mediated bidirectional growth regulation is closely associated with either potentiation or inhibition of RB protein phosphorylation and the histone H 1 kinase activity of cyclin dependent kinases ( cdks ) cdc 2 and cdk 2 , which normally accumulate along the G 1 to the S phase transition . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| RB and Cdc 2 expression in brain : correlations with 3H thymidine incorporation and neurogenesis . ^^^ Intense RB IR and cdc 2 IR were also detected in proliferating neuronal precursor cells in the subgranular region of the dentate gyrus and in the subependymal region extending from the anterior lateral ventricle into the olfactory bulb . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| In vitro biochemical studies with purified PP 1 and PP2A showed that intact RB protein phosphorylated by cdc 2 kinase served as a substrate for both protein phosphatases . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| Using the cell line , we investigated the expression of cell cycle associated proteins including cdc 2 , cdk 2 , cyclin A , cyclin Dl , and Rb during entry into or withdrawal from the cell cycle . ^^^ The expression of cdk 2 and cyclin D 1 in the quiescent cells was reduced after stimulation by renewal of the medium and then increased , accompanied by Rb phosphorylation and cdc 2 expression around the G1 / S transition . ^^^ Cdc 2 and the hyperphosphorylated form of Rb disappeared as the cells became quiescent . ^^^ The results indicate that down regulation of neither cdk 2 , cyclin A , cyclin D 1 , nor MAP kinase is necessary to arrest cells in G 0 , but that only Rb dephosphorylation and down regulation of cdc 2 are accompanied by an arrest of cell proliferation in G 0 in the cell line . . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| IFN gamma caused a rapid ( within 12 24 h ) down regulation of cyclin A , c myc , and cdc 2 proteins , as well as a disappearance of hyperphosphorylated forms of the retinoblastoma family proteins , Rb and p 130 . ^^^ Several mammary carcinoma cell lines demonstrated resistance to the growth inhibitory effects of IFN gamma and did not exhibit down regulation of cdc 2 and cyclin A expression or a change in hyperphosphorylation of Rb when treated with IFN gamma . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| Several nuclear proteins associated with apoptosis , i . e . , p 53 , cdc 2 , and Rb , were absent from PMN . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Analyzing mRNA levels of various epithelial genes ( c myc , cdc 2 kinase , E2F , PCNA , p 53 , and RB , cytokeratins 5 and 10 ) we concluded that MCCs express markers of epithelial hyperproliferation together with markers of neuroendocrine differentiation ( NSE ) . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| Human retinoblastoma protein ( Rb ) is phosphorylated by cdc 2 kinase and MAP kinase in Xenopus maturing oocytes . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| To test this hypothesis , a recently developed quantitative reverse transcriptase polymerase chain reaction method was used to assess simultaneously expression of 15 genes mechanistically associated with cell cycle control ( c myc , E2F 1 , p 21 , rb , PCNA , cyclin D 2 , cyclin D 3 , cyclin E , cdc 2 , CDK 2 , CDK 4 , mad , max p 21 , max p 22 , and p 53 ) in normal cell cultures from five individuals and in nine different malignant BEC lines . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| These results suggest that in human fibroblasts , Ca2+ / calmodulin regulates the signaling cascade leading to cdc 2 kinase activation , RB protein phosphorylation , and DNA synthesis and that this Ca ( 2+ ) dependent regulation is abrogated in SV 40 transformed cells . . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| We therefore focused on factors that are functionally related to activity of the principal cell cycle regulatory element of the histone H 4 promoter : CDC 2 , cyclin A , as well as RB and IRF related proteins . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| Cells that have passed beta selection differ from the preceding cells by several criteria , including hyperphosphorylation of Rb , increased expression of cyclins A and B , down regulation of p 27 , increased CDK 2 activity , an induction of cdc 2 activity , and progression through DNA synthesis . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| Transforming growth factor beta 1 induced growth inhibition of keratinocytes was characterized by decreases in DNA synthesis , accumulation of hypophosphorylated Rb , and the inhibition of the E2F : Rb responsive promoter , cdc 2 , and an induction of the p 21 promoter . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| Fluorescence activated cell sorting measurements and Western blot analysis show DIOA caused a significant reduction of the cell fraction in proliferative phase and a change in phosphorylation of retinoblastoma protein ( Rb ) and cdc 2 , suggesting that KCC 3 activity is important for cell cycle progression . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| METHODS : The molecular expressions of Rb , cyclin A , cyclin E , CDK 2 and CDC 2 involved in S phase progression were investigated by westernblot in L 2 and Br 1 3pr 1 cells . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| P2P R binds the p 53 and the Rb 1 tumor suppressors and is phosphorylated by the cdc 2 and SRPK1a protein kinases . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| We find that active RB mediates histone deacetylation on cyclin A , Cdc 2 , topoisomerase IIalpha , and thymidylate synthase promoters . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| Inhibition of the cdc 2 kinase by roscovitine did not change the phosphorylation status of Rb in SFN treated cells , suggesting that this cyclin dependent kinase may not be involved . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| RXR gamma expression produced significant reduction in levels of RA responsive genes including the cyclin dependent kinase inhibitors p21Cip1 / WAF1 and p27Kip1 , resulting in increased cdc 2 and cdk 2 kinase activity and RB phosphorylation . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| The expression of cell cycle related proteins such as CDK 2 and 4 , cyclin proteins ( D 1 , E and cdc 2 ) , proliferating cell nuclear antigen , tumor suppressor proteins ( p 53 and Rb ) , CDK inhibitory proteins ( p21waf1 and p27Kip1 ) , and apoptosis inhibiting protein ( bcl 2 ) following diethylnitrosamine treatment was examined . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| Surprisingly , tobacco cyclin D and Cdc 2 proteins can form a complex in insect cells , which is able to phosphorylate tobacco Rb related protein in vitro . ^^^ These results suggest that the cdc 2 gene modulates the cell cycle through the phosphorylation of Rb related protein by forming an active complex with cyclin D in plants . . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| Consistent with cell cycle arrest , K252a was shown to hypophosphorylate Rb , upregulate p 21 , and decrease Cdc 2 and Cdc25c activity . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| Treatment with roscovitine or olomoucine , inhibitors of Cdc 2 and Cdk 2 , blocked TGF beta 1 induced apoptosis by inhibiting RB phosphorylation . ^^^ In conclusion , we present the evidence that Cdc 2 and Cdk 2 kinase activity transiently induced by TGF beta 1 phosphorylates RB as a physiological target in FaO cells and that RB hyperphosphorylation may trigger abrupt cell cycle progression , leading to irreversible cell death . . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| However , the Rb hSWI / SNF complex persists and is sufficient to maintain repression of the cyclin A and cdc 2 genes , inhibiting exit from S phase . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| Cyclin dependent kinase ( CDK ) was regulated by CDK inhibitor ( CDKI ) ( p 16 , p 15 ) , and CDK CDKI complex control RB by phosphorylation . p 53 regulate RB pathway through p 21 . p 53 is involved in apoptosis through bcl 2 or Bax 3 . p 19 coded by exon 1 beta and exons 2 and 3 of p 16 gene , binds to MDM 2 and regulate p 53 pathway . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| All retinoids attenuated mitogen induced downregulation of CDKI p 27 ( Kip 1 ) , a major negative regulator of Rb phosphorylation , partly through stabilizing p 27 ( Kip 1 ) turnover . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| The expression of the cyclin dependent kinase inhibitor ( CDKI ) p 27 protein was investigated in relation to ( 1 ) the expression of the cell cycle regulators p 53 , Rb and p 16 and ( 2 ) the proliferation profile as determined by the expression of Ki 67 , cyclin A , and cyclin B 1 in 80 cases of de novo diffuse large B cell lymphomas ( DLBCL ) . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| BMP 7 caused an upregulation of the cyclin dependent kinase inhibitor ( CDKI ) p 21 ( CIP1 / WAF1 ) , and decreased the activity of Cdk 2 , leading to hypophosphorylation of Rb proteins . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| Deregulation of the cell cycle in this state involves reduction of p 21 protein , concomitant upregulation of CDC 2 , and an increase in Rb phosphorylation that favors expression and nuclear translocation of FGFR 4 and FRAG 1 and 2 . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| By contrast , Smad 7 overexpression interfered with TGF beta 1 mediated attenuation of cyclin A and B levels , inhibition of cdc 2 dephosphorylation and CDK 2 inactivation , up regulation of p 27 , and the maintenance of the retinoblastoma protein ( RB ) in a hypophosphorylated state . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| Ponicidin treated MCF 7 cells expressed reduced levels of cyclin B 1 , cdc 2 , transcription factor E2F , and Rb including phosphorylation at S 780 . ^^^ In MCF 10A cells , oridonin was more active than ponicidin in inhibiting the expression of cyclin B 1 , cdc 2 , S 780 phosphorylated Rb , and E2F . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| Levels of p 130 rose after drug treatment , and it was the primary RB family member recruited to the S phase promoters cyclin A and PCNA and G 2 M promoters cyclin B and cdc 2 , remaining present for the entire 8 day time period . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| We observed a gradual decrease of Retinoblastoma protein ( Rb ) phosphorylation and reduced expression of E2F target genes , like Cdc 2 and cyclin A 2 , during embryogenesis and in embryonic fibroblasts ( MEFs ) . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| Although the numbers are very low , none of the samples with an aberrant Rb gene had an altered CDKI and vice versa , suggesting that both genes probably operate in a common pathway and alteration of either can provide these cells with a growth advantage . . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| Mutations in Rb , cyclin D 1 , its catalytic subunit Cdk 4 , and the CDKI p16Ink4a , which alter the protein or its level of expression , are all strongly implicated in cancer . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| Ectopic over expression of the p 16 ( INK4A ) CDKI can inhibit growth of cell lines depending on Rb status . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| Downstream in CDKI CDK cyclin cascade , consistent with its inhibitory effect on CDK kinase activity , IP 6 treatment of cells increased hypophosphorylated levels of retinoblastoma ( Rb ) with a decrease in Rb phosphorylation at serine 780 , 807 , and 811 sites , and caused a moderate to strong decrease in the levels of transcription factors E2F1 , E2F4 , and E2F5 . ^^^ |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|