| Interacting proteins: Q16254 and P06400 |
Pubmed |
SVM Score :0.53655157 |
| Here , we show that the major G 0 E2F activity of primary human T cells , E2F 4 , is stably bound to the p 130 pocket protein in association with a DP heterodimerization partner . p 130 E2F 4 binding has functional implications because p 130 effectively suppressed E2F 4 mediated trans activation , and coexpression of E2F4 overcame p 130 mediated G 1 arrest more efficiently than RB induced G 1 blockade . 0.53655157^^^ |
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| Interacting proteins: Q16254 and P06400 |
Pubmed |
SVM Score :0.0 |
| In contrast , IFN alpha did not affect the DNA binding ability of E2F 1 and E2F 4 in a complex with retinoblastoma ( RB ) susceptibility gene family proteins including pRB , p 107 , and p 130 . ^^^ |
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| Interacting proteins: Q16254 and P06400 |
Pubmed |
SVM Score :0.0 |
| These activities correlate with the appearance of hyperphosphorylated p 130 , an Rb family member important in regulating E2F 4 and E2F 5 activity in fibroblastic cells . ^^^ |
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| Interacting proteins: Q16254 and P06400 |
Pubmed |
SVM Score :0.0 |
| TSA enhanced the protein expression of p 21 ( WAF 1 ) , CREB binding protein , cyclinE , cyclin A , Bak and Bax , while it reduced the expression of E2F 1 , E2F 4 , HDAC 1 , p 53 and hyperphosphorylated form of Rb . ^^^ |
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| Interacting proteins: Q16254 and P06400 |
Pubmed |
SVM Score :0.0 |
| Genes examined included the retinoblastoma susceptibility gene ( Rb 1 ) ; cyclins D 1 , D 2 , A , and E ; the CDK inhibitors p 18 , p 19 , and p 27 ; CDK 2 and CDK 6 ; transcription factors E2F 4 , E2F 5 , and DP 1 ; and the neurofibromatosis type 2 gene . ^^^ |
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| Interacting proteins: Q16254 and P06400 |
Pubmed |
SVM Score :0.0 |
| The major species of E2F in quiescent cells is a complex containing the E2F4 product in association with the Rb related p 130 protein . ^^^ E2F4 then associates with the Rb related p 107 protein as cells enter S phase . ^^^ Rb can be found in interactions with each E2F species , including E2F4 , during G 1 , but there appears to be a limited amount of Rb with respect to E2F , likely due to the maintenance of most Rb protein in an inactive state by phosphorylation . ^^^ As these cells exit the cell cycle , active Rb protein appears to exceed E2F , as there is a marked accumulation of E2F Rb interactions , involving all E2F species , including E2F4 , which is paralleled by the conversion of Rb from a hyperphosphorylated state to a hypophosphorylated state . ^^^ |
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| Interacting proteins: Q16254 and P06400 |
Pubmed |
SVM Score :0.0 |
| The CHR mutated promoter is activated by the E2F family of proteins ( E2F1 , E2F2 , E2F3 , and E2F4 ) but unable to be repressed by any of the Rb family members ( Rb , p 107 , and p 130 ) to the level of the wild type promoter activity in G 0 , indicating that the repression by the Rb family members is required for the corepressor element . ^^^ Moreover , it was shown that a factor specifically bound to the CHR element is co purified with E2F by DNA affinity purification and co immunoprecipitated with E2F4 and the Rb family members . ^^^ |
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| Interacting proteins: Q16254 and P06400 |
Pubmed |
SVM Score :0.0 |
| Immunoprecipitation and subcellular fractionation studies demonstrated that both the nuclear localization of E2F4 and its association with the Rb family member p 130 increased following neuronal differentiation . ^^^ |
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| Interacting proteins: Q16254 and P06400 |
Pubmed |
SVM Score :0.0 |
| But , unlike the E2F4 and E2F5 proteins , which are also expressed in quiescent cells and form complexes with the p 130 protein , the E2F3b protein associates with Rb and represents the predominant E2F Rb complex in quiescent cells . ^^^ |
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| Interacting proteins: Q16254 and P06400 |
Pubmed |
SVM Score :0.0 |
| Expression of the E 2 protein also led to posttranscriptional increase in the level of E2F4 , p 105 ( Rb ) , and p 130 and induced the formation of nuclear E2F4 p 130 and E2F4 p 105 ( Rb ) complexes . ^^^ This resulted in marked rearrangement of the protein complexes that formed at the distal E2F site in the cdc25A promoter , including the replacement of free E2F complexes with E2F4 p 105 ( Rb ) complexes . ^^^ These experiments indicated that repression of E2F responsive promoters following HPV E6 / E7 repression was mediated by activation of the Rb tumor suppressor pathway and the assembly of repressing E2F4 Rb DNA binding complexes . ^^^ |
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| Interacting proteins: Q16254 and P06400 |
Pubmed |
SVM Score :0.0 |
| Electrophoretic mobility shift assays and immunoprecipitation experiments demonstrated that the complexes formed by E2F1 and E2F4 and Rb family proteins vary throughout in vitro keratinocyte differentiation . ^^^ |
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| Interacting proteins: Q16254 and P06400 |
Pubmed |
SVM Score :0.0 |
| We also show that in quiescent normal human cells this entire RBP 1 mSIN3 SAP 30 HDAC complex colocalizes with both RB family members and E2F4 in a limited number of discrete regions of the nucleus that in other studies have been shown to represent the initial origins of DNA replication following growth stimulation . ^^^ |
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| Interacting proteins: Q16254 and P06400 |
Pubmed |
SVM Score :0.0 |
| Finally , amounts of p 27 cyclin E and RB 2 / p130 E2F4 complexes were found to increase in CA OV 3 cells growth arrested by RA . ^^^ |
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| Interacting proteins: Q16254 and P06400 |
Pubmed |
SVM Score :0.0 |
| Silibinin treatment of cells resulted in a strong increase ( up to 2 . 3 and 5 . 4 fold ) in the levels of hypophosphorylated Rb / p107 and Rb2 / p130 , respectively , but a strong decrease ( 91 , 78 and 45 % ) in protein levels of transcription factors E2F3 , E2F4 and E2F5 , respectively . ^^^ |
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| Interacting proteins: Q16254 and P06400 |
Pubmed |
SVM Score :0.0 |
| E2F4 loss suppresses tumorigenesis in Rb mutant mice . ^^^ Remarkably , E2F4 loss suppresses the development of both pituitary and thyroid tumors in Rb ( + / ) mice . ^^^ |
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| Interacting proteins: Q16254 and P06400 |
Pubmed |
SVM Score :0.0 |
| In addition , E2F4 loss does not override the differentiation defect resulting from pRB loss even though it completely suppresses the proliferation defect of Rb ( / ) mouse embryonic fibroblasts . ^^^ |
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| Interacting proteins: Q16254 and P06400 |
Pubmed |
SVM Score :0.0 |
| Cell lines with restored cdk 6 levels accumulated higher amounts of the Rb family protein p 130 as well as E2F4 , a suppressing member of the E2F family of transcription factors , in their nuclei . ^^^ |
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| Interacting proteins: Q16254 and P06400 |
Pubmed |
SVM Score :0.0 |
| In conclusion , our data suggest a key role for E2F4 in proliferation of human intestinal crypt cells and that its cytoplasmic retention as well as its sequestration by Rb proteins may represent a critical step in initiating cell cycle exit . . ^^^ |
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| Interacting proteins: Q16254 and P06400 |
Pubmed |
SVM Score :0.0 |
| Downstream in CDKI CDK cyclin cascade , consistent with its inhibitory effect on CDK kinase activity , IP 6 treatment of cells increased hypophosphorylated levels of retinoblastoma ( Rb ) with a decrease in Rb phosphorylation at serine 780 , 807 , and 811 sites , and caused a moderate to strong decrease in the levels of transcription factors E2F1 , E2F4 , and E2F5 . ^^^ |
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| Interacting proteins: Q16254 and P06400 |
Pubmed |
SVM Score :0.0 |
| The basis for this transformation resistance is a perturbed function of the C terminal Rb binding region of E2F4 . ^^^ However , inactivation of Rb or substitution of the E2F4 C terminal domain by the E2F1 C terminal region rescues the transformation defect . ^^^ |
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| Interacting proteins: Q16254 and P06400 |
Pubmed |
SVM Score :0.0 |
| Here we show that p 130 is the predominant Rb family member associated with E2F in neurons , that its major partner for repression of pro apoptotic genes is E2F4 , and that the p 130 E2F4 complex recruits the chromatin modifiers HDAC 1 and Suv39H1 to promote gene silencing and neuron survival . ^^^ |
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| Interacting proteins: Q16254 and P06400 |
Pubmed |
SVM Score :0.0 |
| Western blot studies showed that expression of cyclin D 1 , CDK 2 , and E2F4 were increased , and Rb levels were decreased in these cells . ^^^ |
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| Interacting proteins: Q16254 and P06400 |
Pubmed |
SVM Score :0.0 |
| Using neocortical extracts , we detected GA binding protein alpha ( GABPalpha , an Ets family member ) bound to the activator site and both E2F1 and E2F4 bound to the repressor site of the Rb promoter in vitro . ^^^ Additionally , we detected binding of both E2F1 and E2F4 to the Rb promoter in vivo using chromatin immunoprecipitation analysis on embryonic day 13 . 5 brain . ^^^ |
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| Interacting proteins: Q16254 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q16254 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q16254 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q16254 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q16254 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q16254 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q16254 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q16254 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q16254 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q16254 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q16254 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q16254 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q16254 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q16254 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q16254 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q16254 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q16254 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
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