| Interacting proteins: Q14186 and P06400 |
Pubmed |
SVM Score :0.0 |
| DRTF 1 , in addition , associates with the tumour suppressor retinoblastoma ( Rb ) gene product and the Rb related protein p 107 . ^^^ We now show , using biologically active fusion proteins , that cyclin A can direct the binding of the cdc 2 like kinase subunit , p33cdk2 , to complexed DRTF 1 , containing either Rb or p 107 , as well as activate its histone H 1 kinase activity . ^^^ |
|
| Interacting proteins: Q14186 and P06400 |
Pubmed |
SVM Score :0.0 |
| The retinoblastoma ( Rb ) gene product forms a complex with the cellular transcription factor DRTF 1 , a property assumed to be important for mediating negative growth control because certain viral oncogenes , such as adenovirus E1a , prevent this interaction and mutant Rb alleles , which have lost the capacity to regulate growth , encode proteins that fail to associate with DRTF 1 . ^^^ In this study , we show that the wild type Rb protein can specifically repress transcription from promoters driven by DRTF 1 whereas a naturally occurring mutant Rb protein can not . ^^^ The Rb protein therefore acts in trans to repress the transcriptional activity of DRTF 1 whereas adenovirus E1a prevents this interaction and thus maintains DRTF 1 in a constitutively active state . ^^^ |
|
| Interacting proteins: Q14186 and P06400 |
Pubmed |
SVM Score :0.0 |
| The retinoblastoma tumor suppressor gene encodes a 105 kDa nuclear phosphoprotein ( RB ) that can associate with DRTF 1 and E2F . ^^^ We found that the highest affinity motifs recognized by RB containing complexes are related to the E2F / DRTF 1 binding site and fall into three classes : TTTTCCCGCCAAAA , TTTTCCCGCCTTTTTT or TTTTCCCGCGCTTTTTT . ^^^ |
|
| Interacting proteins: Q14186 and P06400 |
Pubmed |
SVM Score :0.0 |
| The retinoblastoma gene ( Rb ) product is a negative regulator of cellular proliferation , an effect that could be mediated in part at the transcriptional level through its ability to complex with the sequence specific transcription factor DRTF 1 . ^^^ Cyclin A , which also complexes with DRTF 1 , facilitates the efficient assembly of the Rb protein into the complex . ^^^ Here we report on a naturally occurring loss of function Rb allele encoding a protein that fails to complex with DRTF 1 . ^^^ |
|
| Interacting proteins: Q14186 and P06400 |
Pubmed |
SVM Score :0.0 |
| DRTF 1 is a cellular transcription factor which complexes with the retinoblastoma ( Rb ) gene product and cyclin A , an association modulated by certain viral oncogenes , such as adenovirus E1a . ^^^ Complexed DRTF 1 , referred to as DRTF1a , has similar DNA binding specificity and DNA binding polypeptides to DRTF1b , which lacks Rb . ^^^ Consistent with the relationship between EC , ES and inner cell mass cells , DRTF1b is present in blastocyst stage embryos although as embryogenesis progresses the levels of Rb complexed DRTF 1 increase . ^^^ Certain tissues , such as liver and brain , contain high levels of DRTF 1 during early embryonic stages but little in adult terminally differentiated tissue , in contrast to the thymus , which contains high levels of Rb complexed DRTF 1 but lacks associated cyclin A . ^^^ These data show that DRTF 1 is a group of transcription factors that share common DNA binding polypeptides and which complex with other non DNA binding proteins , such as the Rb protein and cyclin A , in a developmentally regulated and tissue dependent fashion . . ^^^ |
|
| Interacting proteins: Q14186 and P06400 |
Pubmed |
SVM Score :0.0 |
| Here we report the isolation of a complementary DNA encoding DRTF 1 polypeptide 1 ( DP 1 ) , a major sequence specific binding protein that is present in DRTF1 / E2F , including Rb and p 107 associated DRTF1 / E2F . ^^^ |
|
| Interacting proteins: Q14186 and P06400 |
Pubmed |
SVM Score :0.0 |
| Interestingly , the binding of E 4 6 / 7 and the binding of Rb to E2F are mutually exclusive , and our results show that the same internal segments of E2F 1 and DP 1 that are required for E 4 6 / 7 binding are also required for stable interaction with Rb . ^^^ |
|
| Interacting proteins: Q14186 and P06400 |
Pubmed |
SVM Score :0.0 |
| Regulation of adenovirus 12 E1A transcription : E2F and ATF motifs in the E1A promoter bind nuclear protein complexes including E2F1 , DP 1 , cyclin A and / or RB and mediate transcriptional ( auto ) activation . ^^^ For HeLa , 293 , U 937 , and A 549 cells , participation of E2F 1 , DP 1 , cyclin A , and RB was involved in formation of some complexes only , assuming participation of factors different from E2F 1 or DP 1 in others . ^^^ |
|
| Interacting proteins: Q14186 and P06400 |
Pubmed |
SVM Score :0.0 |
| E2F 1 forms a heterodimer with DP 1 and binds to several cell cycle regulatory proteins , including the retinoblastoma family ( RB , p 107 , p 130 ) and cyclin A / CDK2 complexes . ^^^ Because the stable association of E2F 1 with cyclin A / CDK2 in vitro and in vivo did not require a DP 1 or RB binding domain and because the interactions could be reconstituted from purified components in vitro , we conclude that the interactions between cyclin A / CDK2 and E2F 1 are direct . ^^^ |
|
| Interacting proteins: Q14186 and P06400 |
Pubmed |
SVM Score :0.0 |
| There is also recent evidence which suggests that mdm 2 may play roles in p 53 independent pathways regulating cellular proliferation . mdm 2 has recently been shown to interact with the retinoblastoma tumor suppressor protein p ( Rb ) , and the E2F 1 and DP 1 transcription factors . ^^^ |
|
| Interacting proteins: Q14186 and P06400 |
Pubmed |
SVM Score :0.0 |
| Using recombinant E2F , DP , and Rb proteins prepared in baculovirus infected cells and a repetitive immunoprecipitation PCR procedure ( CASTing ) , we have identified consensus DNA binding sites for E2F 1 / DP 1 , E2F 1 / DP 2 , E2F 4 / DP 1 , and E2F 4 / DP 2 complexes as well as an Rb / E2F 1 / DP 1 trimeric complex . ^^^ |
|
| Interacting proteins: Q14186 and P06400 |
Pubmed |
SVM Score :0.0 |
| Genes examined included the retinoblastoma susceptibility gene ( Rb 1 ) ; cyclins D 1 , D 2 , A , and E ; the CDK inhibitors p 18 , p 19 , and p 27 ; CDK 2 and CDK 6 ; transcription factors E2F 4 , E2F 5 , and DP 1 ; and the neurofibromatosis type 2 gene . ^^^ |
|
| Interacting proteins: Q14186 and P06400 |
Pubmed |
SVM Score :0.0 |
| The expressions of N myc , cyclin D 3 , and Wnt10B were downregulated , whereas those of retinoblastoma ( RB ) and related genes ( p 107 , RB2 / p130 , p300 / CBP , E2F 1 , DP 1 ) as well as others were upregulated . ^^^ |
|
| Interacting proteins: Q14186 and P06400 |
Pubmed |
SVM Score :0.0 |
| Thus , Dp 1 is largely dispensable for embryonic development , despite the absolute extraembryonic requirement for Dp 1 , which is highly reminiscent of the restricted roles for Rb and cyclins E1 / E2 in vivo . . ^^^ |
|
| Interacting proteins: Q14186 and P06400 |
Pubmed |
SVM Score :0.0 |
| It is clear that G 1 S phase control is exerted after the mouse embryo implants into the uterus 4 . 5 days after fertilization ( E4 . 5 ) ; null mutants of genes that control cell cycle commitment such as max , rb ( retinoblastoma ) , and dp 1 are embryonic lethal after implantation with proliferation phenotypes . ^^^ In order to understand when embryos might first exert G 1 S phase regulatory control , we assayed preimplantation mouse embryos for the acquisition of expression of mRNA , protein , and phospho protein for max , Rb , and DP 1 , and for the proliferation promoting phospho protein forms of mycC ( thr58 / ser62 ) and Rb ( ser 795 ) . ^^^ The key findings are that : ( 1 ) DP 1 protein was present in the nucleus as early as the four cell stage onwards , ( 2 ) max protein was in the nucleus , suggesting function from the four cell stage onwards , ( 3 ) both mycC and Rb all form protein was present at increasing quantities in the cytoplasm from the 2 cell and 4 / 8 cell stage , respectively , ( 4 ) the phosphorylated form of mycC phospho was present in the nucleus at high levels from the two cell stage through blastocyst stage , and ( 5 ) the phosphorylated form of Rb was detected at low levels in the two cell stage embryo and was highly expressed at the 4 / 8 cell stage through the blastocyst stage . ^^^ Taken together , these data suggest that activation of mycC phospho / max dimer pairs , ( E2F ) / DP 1 dimer pairs , and repression of Rb inhibition of cell cycle progression via phosphorylation at ser 795 occurs at the earliest stages of embryonic development . ^^^ In addition , the presence of max , mycC phospho , DP 1 , and Rb phospho in the nuclei of embryonic and placental lineage cells in the blastocyst and in trophoblast stem cells suggests that a similar type of cell cycle regulation is present throughout preimplantation development and in both embryonic and extra embryonic cell lineages . . ^^^ |
|
| Interacting proteins: Q14186 and P06400 |
Pubmed |
SVM Score :0.0 |
| Structure of the Rb C terminal domain bound to E2F1 DP 1 : a mechanism for phosphorylation induced E2F release . ^^^ |
|
| Interacting proteins: Q14186 and P06400 |
Pubmed |
SVM Score :0.0 |
| We also found coinduction of several groups of related genes that might represent functional modules within the ischemic neuronal transcriptome , including VEGF and its receptor , NRP 1 ; the IGF 1 receptor and the IGF 1 binding protein IGFBP 2 ; Rb , the Rb binding protein E2F1 , and the E2F related transcription factor , TFDP 1 ; the CACNB 3 and CACNB 4 beta subunits of the voltage gated calcium channel ; and caspase 3 and its substrates , ACINUS , FEM 1 , and GSN . ^^^ |
|
| Interacting proteins: Q14186 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14186 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14186 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14186 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14186 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14186 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14186 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14186 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14186 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14186 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14186 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14186 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|