| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| In addition , increased expression of the mdm 2 oncogene and both the truncated and the wild type RB protein were noted in BON . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| IHC positivity was found for the tumor suppressor protein Rb and the oncogene product MDM 2 . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| In total , 11 / 12 MPNSTs showed DNA changes in one or more of the genes CDKN2A , CDKN2B , RB 1 , CDK 4 , MDM 2 , and CCND 2 . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| RB , MDM 2 ) , DNA damage recognition and repair ( e . g . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| TRs affects also the action of certain protooncogenes ( Mdm 2 ) and tumor suppressors ( p 53 , Rb ) . . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.98376698 |
| There is also recent evidence which suggests that mdm 2 may play roles in p 53 independent pathways regulating cellular proliferation . mdm 2 has recently been shown to interact with the retinoblastoma tumor suppressor protein p ( Rb ) , and the E2F 1 and DP 1 transcription factors . 0.98376698^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.5229549 |
| In addition to binding to and inhibiting the transcriptional activation function of the p 53 protein , MDM 2 binds , inter alia , to RB and the E2F 1 . 0.5229549^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.85301766 |
| The molecular interactions between mdm 2 , pS 3 and Rb in cancer , are associated with a loss of control in the G 1 phase of the cell cycle leading to uncontrolled cell proliferation . 0.85301766^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.69795299 |
| In this study , we demonstrate that MDM 2 specifically binds to Rb C pocket and that the central acidic domain of MDM 2 is essential for Rb interaction . 0.69795299^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.60591138 |
| MDM 2 also interacts with Rb through its central acidic domain and inhibits Rb function in part by blocking Rb E2F DNA complex formation . 0.60591138^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Immunohistochemistry for Rb , p 16 , cyclin D 1 , p 53 , HDM 2 , and Bcl 2 was performed on twelve eyes containing posterior uveal melanomas that were enucleated following plaque radiotherapy . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| A multiparametric analysis identified proteins associated with increased growth fraction ( Hdm 2 , p 53 , p 21 , Rb , cyclins A , B 1 , D 3 , and E , CDK 2 , CDK 6 , SKP 2 , Bcl 10 ( L ) , survivin , STAT 1 , and STAT 3 ) , and proteins associated with apoptosis ( NF kappaB , STAT 1 , and RB ) . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Here , we have further investigated the functional consequences of this cleavage on two Rb binding partners : the oncoprotein Mdm 2 and the transcription factor E2F 1 . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| To establish the utility of such cell lines as indicators of change relevant to urothelial cancer , we have characterised the expression of five genes ( p 53 , MDM 2 , Rb , E cadherin , APC ) within a panel of human bladder carcinoma cell lines . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| In this study we extend the genetic analysis of the in vivo SGH model to increase the number of cases analyzed for the status of K ras and to determine further the spectrum of alterations involved ; we have studied the status of the p 53 , DCC , and Rb 1 suppressor genes and the status of the mdm 2 oncogene , which can involve p 53 indirectly . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| The present report summarizes the current molecular biological aspects of several oncogenes ( erbB , ras , myc , raf , fos , jun , bcl , mdm 2 , myb , kit CSF1R , met ) and tumor suppressor genes ( p 53 , rb , mts ) involved in lung cancer development with respect to the pathology of lung tumors , including the importance of these genes as far as the clinical course of the disease is concerned . . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| A normal accumulation of p 53 , p21Cip1 / Waf1 , and Mdm 2 proteins and hypophosphorylation of Rb protein occurred in response to radiation with only small differences noted in the kinetics of p 53 and p21Cip1 / Waf1 induction . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Alterations of the p 53 , Rb and MDM 2 genes in osteosarcoma . ^^^ In this study , we have assessed the occurrence of p 53 , Rb and MDM 2 alterations in the same samples of osteosarcomas , along with representative samples of various other sarcomas . ^^^ In summary , 34 alterations of the p 53 , Rb and MDM 2 genes were found in 26 of 42 ( 62 % ) osteosarcomas . . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Two main anomalies were found : ( 1 ) One group of 21 / 130 cases with partial or total loss of Rb protein expression , which could reflect the existence of genetic alterations , or an altered transcriptional or translational regulation of Rb gene . ( 2 ) Another group with an abnormally high Rb / Ki67 ratio , which could support conflicting interpretations : ( 1 ) excess Rb protein for controlling cell cycle progression ; or ( 2 ) adhesion of Rb protein to other cellular or viral proteins , such as p 53 and MDM 2 . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Deletion of four residues from the E2F1 activation domain reduces CBP binding as well as transcriptional activation , but still allows the binding of RB and MDM 2 . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| In addition , in cells lacking the function of the Rb tumor suppressor , the apoptotic activity of p 53 could be inhibited by reconstitution of active Rb . p 53 mediated apoptosis could also be inhibited by the protein encoded by the mdm 2 oncogene . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Cleaved Rb bound cyclin D 3 and inhibited the transcriptional activity of E2F 1 , but failed to bind to the regulatory protein MDM 2 , which has been implicated in apoptosis . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Induction of DNA damage in normal , uninfected fibroblasts ( FB ) or FB expressing IE 86 by actinomycin D ( Act D ) resulted in increased p 53 levels , a predominance of the hypophosphorylated form of Rb , and increased expression of both p 21 ( CIP1 / WAF1 ) and mdm 2 . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| E2F1 induced apoptosis can be specifically inhibited by Rb but not mdm 2 , which is known for its ability to inhibit p 53 induced apoptosis . ^^^ The transactivation defective E2F1 mutants E2F1 ( 1 374 ) , E2F1 ( 390 1 ) DF ( delta mdm 2 ) , and E2F1 ( 406 415 ) ( delta Rb ) can induce apoptosis as effectively as wild type E2F1 . ^^^ This hypothesis was supported by our observation that although Rb overexpression can specifically repress the apoptosis induced by wild type E2F1 and a Rb binding competent E2F1 mutant E2F1 ( 390 1 ) DF ( delta mdm 2 ) , it failed to inhibit the apoptosis induced by mutants E2F1 ( 1 374 ) and E2F1 ( delta 406 415 ) ( delta Rb ) , which are defective or reduced in Rb binding and transactivation . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| These alterations lead to changes in the expression of several genes ; protein 53 ( p 53 ) , retinoblastoma ( RB ) , interferon ( INF ) alpha / beta , cyclic AMP dependent kinase number 2 ( CDKN 2 ) , mutated in multiple advanced cancers 1 ( MMAC 1 ) , deleted in colon carcinoma ( DCC ) , epidermal growth factor receptor ( EGFR ) , platelet derived growth factor ( PDGF ) , platelet derived growth factor receptor ( PDGFR ) , MDM 2 , GL 1 , CDK 4 and SAS during the genesis and progression of human gliomas . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| It is possible that abrogation of Rb function by E 7 or increased expression of MDM 2 contributes to the loss of mitotic spindle checkpoint control in the E 7 cells . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Rb and mdm 2 oncoproteins . ^^^ OBJECTIVE : To determine the expression of p 53 , p 21 , Rb and mdm 2 proteins in normal placentas , partial and complete hydatidiform moles , and choriocarcinomas and to examine possible p 53 mutations in specimens from p 53 positive cases . ^^^ CONCLUSION : Altered expression of p 53 , p 21 , Rb and mdm 2 may be important in the pathogenesis of both complete mole and choriocarcinoma . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| The results suggest that MDM 2 mediated resistance to p 53 may be bypassed by p 21 and that the Rb phosphorylation state may predict the effects on growth after Ad p 53 or Ad p 21 infection . . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| ARF promotes MDM 2 degradation and stabilizes p 53 : ARF INK4a locus deletion impairs both the Rb and p 53 tumor suppression pathways . ^^^ Thus , deletion of the ARF INK4a locus simultaneously impairs both the INK4a cyclin D / CDK4 RB and the ARF MDM 2 p53 pathways . . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Twenty primary central nervous system lymphomas ( PCNSL ) from immunocompetent patients ( nineteen B cell lymphomas and one T cell lymphoma ) were investigated for genetic alterations and / or expression of the genes BCL 2 , CCND 1 , CDK 4 , CDKN1A , CDKN2A , MDM 2 , MYC , RB 1 , REL , and TP 53 . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Molecular alterations of the RB 1 , TP 53 , and MDM 2 genes in primary and xenografted human osteosarcomas . ^^^ We report the status of the RB 1 , TP 53 , and MDM 2 genes in human osteosarcomas and cell lines established from surgical specimens and transplanted into athymic naked mice . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| PATIENTS AND METHODS : To this end , we collected the clinical data of 141 patients with DLBCL and immunohistochemically analyzed diagnostic tumoral tissue from each patient for the presence of Ki 67 ( MIB 1 , Immuno tech , Marseille , France ) , bcl 2 , p 53 , p21 / WAF1 , MDM 2 , and retinoblastoma ( Rb ) proteins . ^^^ Multivariate analysis showed that an extended overall survival ( OS ) time was associated with low growth fraction , high Rb protein , and low MDM 2 expression , as well as with known clinical parameters . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| The growth regulatory function of the retinoblastoma protein ( RB ) can be suppressed by mdm 2 via RB / mdm2 interaction by perturbation of the RB suppression of the E2F function . ^^^ The goal of this study was to examine the clinical value of immunohistochemical ( IHC ) RB detection and its relationship to mdm 2 overexpression in a cohort of 198 adult primary soft tissue sarcomas ( STS ) . ^^^ RB overexpression reveals , multivariately , a correlation with survival ( RR=1 . 59 , P=0 . 037 ) as well as mdm 2 positivity ( RR=2 . 32 , P=0 . 0035 ) . ^^^ Stratification of RB results to mdm 2 staining shows a prognostic graduation in four levels . ^^^ Intermediately , an isolated RB overexpression ( projected 5 year survival rate , 46 . 1 % ) is more favourable than an isolated mdm 2 positivity ( projected 5 year survival rate , 33 . 5 % ) . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Treatment with mitomycin C or adriamycin did not result in accumulation of p 53 or induction of p21WAF1 / CIP1 or Mdm 2 and did not cause Rb dephosphorylation . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Differential p 53 , p 21 , mdm 2 and Rb regulation in glioma cell lines that overexpress wild type p 53 . ^^^ We have utilized a series of glioma cell lines to study the effects of ionizing radiation on the regulation of p 53 , p 21 , mdm 2 and Rb proteins . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| The sensitivity to preoperative chemotherapy was investigated with reference to loss of heterozygosity ( LOH ) at the 17p13 ( p 53 ) and 13q14 ( Rb ) loci and expression of the cell cycle associated proteins , p 53 , Rb , p21 / Waf 1 , mdm 2 and Ki 67 , as detected immunohistochemically . ^^^ No relationship was evident between chemosensitivity and immunohistochemical staining patterns for p 53 , Rb , p21 / Waf 1 , mdm 2 or Ki 67 . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Overexpression of Ki 67 , p 53 , and mdm 2 and deletion of Rb were determined via standard IHC techniques on serial paraffin sections . ^^^ Overexpression of p 53 of mdm 2 or deletion of Rb did not correlate with increased risk of DM or TM alone or in combination with a Ki 67 score of > or = 20 . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Expression of p 53 , p 21 , mdm 2 , Rb , bax and Ki 67 proteins in lymphomas of the mucosa associated lymphoid ( MALT ) tissue . ^^^ We have investigated by immunohistochemistry 38 cases of B cell MALT NHL comprising 23 high grade ( HG ) and 15 low grade ( LG ) tumours for the expression of p 53 , mdm 2 , p 21 , Rb , Ki 67 , bcl 2 and Bax proteins . ^^^ These findings show that significant expression of p 53 , mdm 2 , p 21 , Ki 67 and Rb proteins occurs more frequently in aggressive histotypes of MALT NHL . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| The INK4a / ARF locus sits at the nexus of these two growth control pathways , by virtue of its ability to generate two distinct products : the p16INK4a protein , a cyclin dependent kinase inhibitor that functions upstream of RB ; and the p19ARF protein , which blocks MDM 2 inhibition of p 53 activity . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Statistical analysis of immunohistochemical data from 216 patients did not identify significant or consistent differences of p 53 , MDM 2 , or RB expression with respect to stage of disease , malignant transformation , metastatic node involvement , recurrence , or survival . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| The Mdm 2 protein is believed to be oncogenic by binding and inactivating the p 53 and Rb tumor suppressor gene products and by activating the E2F 1 / DP 1 transcription factors , thus promoting the G 1 to S phase transition . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| MDM 2 amplification and loss of heterozygosity at Rb and p 53 genes : no simultaneous alterations in the oncogenesis of liposarcomas . ^^^ PURPOSE : The present study aimed to investigate the status of alterations of the MDM 2 , Rb and p 53 genes in a series of 45 liposarcomas . ^^^ In contrast to myxoid and pleomorphic variants , well differentiated liposarcomas were characterized by a high frequency of MDM 2 amplification , a lack of LOH of Rb and p 53 , and p 53 mutations . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Recent studies provide evidence that these pathways are directly linked via MDM 2 pRb interaction and p 53 suppression of the RB 1 gene . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Thus , MDM 2 may confer TGF beta resistance in a subset of tumors and may promote tumorigenesis by interference with two independent tumor suppressors , p 53 and Rb . . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Status of the p 53 , Rb and MDM 2 genes in canine osteosarcoma . ^^^ To study whether this similarity extends to the molecular level , 21 canine osteosarcomas were analyzed for alterations of p 53 , Rb and MDM 2 . ^^^ The gross structure of the p 53 , Rb and MDM 2 genes was examined by Southern blotting . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| The central role of cell cycle regulation has been demonstrated in hereditary ( p 53 , RB ) and somatic mutations or variant expression of genes associated with cell cycle regulation ( p 53 , BCL 2 , MDM 2 ) . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| RB regulates the stability and the apoptotic function of p 53 via MDM 2 . ^^^ The binding of RB to MDM 2 is shown to be essential for RB to overcome both the antiapoptotic function of MDM 2 and the MDM 2 dependent degradation of p 53 . ^^^ The RB MDM 2 interaction does not prevent MDM 2 from inhibiting p 53 dependent transcription , but the RB MDM 2 complex still binds to p 53 . ^^^ Since RB specifically rescues the apoptotic function but not the transcriptional activity of p 53 from negative regulation by MDM 2 , transactivation by wild type p 53 is not required for the apoptotic function of p 53 . ^^^ However , an RB MDM 2 p53 trimeric complex is active in p 53 mediated transrepression . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| The two gene products of the CDKN2A gene , p 16 and p19ARF , have recently been linked to each of two major tumour suppressor pathways in human carcinogenesis , the RB 1 pathway and the p 53 pathway . p 16 inhibits the phosphorylation of the retinoblastoma gene product by cyclin D dependent kinases , whereas p19ARF targets MDM 2 , a p 53 inhibitory protein , for degradation . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| An immunochemical analysis of mdm 2 expression in human breast cancer and the identification of a growth regulated cross reacting species p 170 . mdm 2 is a 491 amino acid nuclear protein which is involved in complex interactions with important cell cycle and stress response regulators including p 53 , Rb and E2F . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Aberrant expression of the cell cycle associated proteins TP 53 , MDM 2 , p 21 , p 27 , cdk 4 , cyclin D 1 , RB , and EGFR in cervical carcinomas . ^^^ OBJECTIVE : The aims of this study were to study aberrant expression and coexpression of the cell cycle associated proteins TP 53 , p 21 , p 27 , cyclin D 1 , cdk 4 , RB , EGFR , and MDM 2 in cervical carcinomas , to correlate protein alterations with histopathological and clinical parameters , and to evaluate whether these alterations provide prognostic information . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| However , the identification of the mdm 2 Rb interaction established a closer link between the two most well studied tumour suppressors , p 53 and Rb . ^^^ Recent studies of the novel trimeric complex Rb mdm 2 p53 provided us with a functional insight of how the two tumour suppressors can act together in regulating p 53 induced apoptosis . ^^^ Beginning with the properties of the Rb mdm 2 p53 trimeric complex , we shall review the propounding evidence suggesting that the apoptotic function of p 53 is linked to its transrepression function . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Rb , and MDM 2 proteins in tongue carcinoma from patients < 35 versus > 75 years . ^^^ The purpose of this investigation was to evaluate cell cycle proteins ( p 53 , p 21 , Rb , MDM 2 ) in lateral tongue cancers from patients at the two ends of the age spectrum . ^^^ We conclude that p 53 , p 21 , Rb , and MDM 2 are over expressed in lateral tongue cancers , and that immunohistochemical profiles are heterogeneous . ^^^ Significant differences in the expression of p 53 , p 21 , Rb , and MDM 2 proteins are not evident in lateral tongue carcinomas from patients < 35 years as compared to patients > 75 years . . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Loss of p16INK4a expression would disrupt the retinoblastoma ( Rb ) / p16INK4a / cyclin D dependent kinase ( CDK 4 ) pathway , whereas loss of p14ARF expression would inactivate both the Rb and p53 / MDM2 / p14ARF pathways through MDM 2 , which can complex with either Rb or p 53 . ^^^ |
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| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| In this paper , the presence of SV 40 like sequences was investigated in 54 Japanese osteosarcomas in which mutations of the retinoblastoma ( Rb ) , p 53 , MDM 2 , and CDK 4 genes had been already analysed . ^^^ |
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| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| We have analyzed the expression of the CDKN1A ( p 21 ( CIP 1 ) ) , CDKN1B ( p 27 ( Kip 1 ) ) , TP 53 , RB 1 and MDM 2 proteins and tumor cell proliferation by immunohistochemical staining in 59 cases of metastatic melanoma . ^^^ We found positive correlations between the expression of CDKN1A and MDM 2 ( r = 0 . 5063 , P = 0 . 001 ) , between the expression of CDKN1B and RB 1 ( r = 0 . 5026 , P = 0 . 001 ) , and between RB 1 expression and tumor cell proliferation ( 0 . 5564 , P < 0 . 001 ) . ^^^ |
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| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| TP 53 alterations in relation to the cell cycle associated proteins p 21 , cyclin D 1 , CDK 4 , RB , MDM 2 , and EGFR in cancers of the uterine corpus . ^^^ In the present study , TP 53 alterations have been analysed and compared with the expression of the proteins p 21 , cyclin D 1 , cdk 4 , RB , EGFR , and MDM 2 in 53 cancers of the uterine corpus . ^^^ |
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| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Cyclin dependent kinase ( CDK ) was regulated by CDK inhibitor ( CDKI ) ( p 16 , p 15 ) , and CDK CDKI complex control RB by phosphorylation . p 53 regulate RB pathway through p 21 . p 53 is involved in apoptosis through bcl 2 or Bax 3 . p 19 coded by exon 1 beta and exons 2 and 3 of p 16 gene , binds to MDM 2 and regulate p 53 pathway . ^^^ |
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| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Lesions in the p 16 cyclin D CDK 4 Rb and ARF Mdm 2 p 53 pathways occur so frequently in cancer , regardless of patient age or tumor type , that they appear to be part of the life history of most , if not all , cancer cells . . ^^^ |
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| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Fifty three neuroendocrine lung tumors ( 24 carcinoids , one atypical carcinoid , five large cell neuroendocrine carcinomas , and 23 small cell lung carcinomas ) were investigated for immunocytochemical expression of several gene products , i . e . , p 53 , Rb , bcl 2 , c kit , mdm 2 , cdk 4 , p 21 proteins , and proliferation index as assessed by MIB 1 . ^^^ |
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| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Apoptosis was evaluated by TUNEL technique ( TdT mediated dUTP nick end labeling ) and the presence of EBV latent membrane protein 1 as well as expression of Bcl 2 , tumor suppressor p 53 , p21WAF1 , MDM 2 , Rb 1 , PCNA , p27KIP1 and caspase 3 , was detected immunocytochemically on paraffin embedded lymph node specimens obtained at diagnosis . ^^^ On the other hand , no statistically confirmed correlation was found between AI and expression of bcl 2 , MDM 2 , Rb 1 , and p27KIP1 on R S / H cells . ^^^ |
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| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Correlation with p 53 , mdm 2 , Rb proteins and proliferation indices . ^^^ The aim of the study was to determine bcl 2 expression in correlation with p 53 , mdm 2 and Rb expression , with proliferation indices ( Ki 67 LI , PCNA LI ) as well as with conventional clinicopathological variables . ^^^ |
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| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Immunohistochemical expression of retinoblastoma gene product ( Rb ) , p 53 protein , MDM 2 , c erbB 2 , HLA DR and proliferation indices in human urinary bladder carcinoma . ^^^ Archival biopsy specimens from transitional cell bladder cancers ( n=88 ) were analysed immunohistochemically for the expression of the retinoblastoma ( Rb ) gene protein , p 53 , mdm 2 , c erbB 2 , HLA DR antigen and proliferation indices . ^^^ An altered nuclear expression of Rb , p 53 and mdm 2 was observed in 55 . 2 % , 33 . 3 % and 18 . 2 % of tumors respectively . ^^^ |
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| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Rb , an Egr 1 target gene , forms a trimeric complex with p 53 and MDM 2 to prevent MDM 2 mediated p 53 degradation . ^^^ Elevated amounts of the p 53 MDM2 complex and low amounts of Rb MDM 2 complex were observed in Egr 1 ( / ) cells after radiation . ^^^ Because of a reduction in Rb binding to MDM 2 and an increase in MDM 2 binding with p 53 , p 53 is directly degraded by MDM 2 , and this leads to inactivation of the p 53 mediated apoptotic pathway in Egr 1 ( / ) MEF cells . ^^^ Thus , the proapoptotic function of Egr 1 may involve the mediation of Rb protein that is essential to overcome the antiapoptotic function of MDM 2 on p53 . . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| The p 53 dependent tumourigenesis is influenced by interaction not only with E6 / HPV 16 , 18 but also with MDM 2 , bcl 2 and RB protein . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Although p 16 ( INK4a ) and p 15 ( INK4b ) are involved in the phosphorylation of the retinoblastoma ( Rb ) protein , p 14 ( ARF ) interacts with the MDM 2 oncoprotein antagonizing its function as a suppressor of p 53 . ^^^ |
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| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| RB , CDK 4 , MDM 2 , c myc , c fos , and p 53 gene expression , which are the major genes involved in osteosarcoma susceptibility , were studied . ^^^ Our results revealed an inactive form of p 53 sporadically seen in the samples , a total loss of Rb protein expression , an increased expression of Cdk 4 , MDM 2 , c fos , and c myc proteins which literature currently reports being the principal alterations found in osteosarcoma . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Cell growth regulators include proteins of the p 53 pathway encoded by the genes CDKN2A ( p 16 , p14arf ) , MDM 2 , TP 53 , and CDKN1A ( p 21 ) as well as proteins encoded by genes like RB 1 , E2F , and MYCL . ^^^ |
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| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| These results suggest that disruption of the RB and p 53 pathways is a frequent event in SCC , and that abnormal expression of p 16 and p 53 plays a more critical role than that of pRB , p14ARF and MDM 2 in the development of SCC of the lung . . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Alternative and aberrant messenger RNA splicing of the mdm 2 oncogene in invasive breast cancer . mdm 2 is part of a complex mechanism that regulates the expression of p 53 as well as the function of Rb , p19ARF , and other genes . ^^^ |
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| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| The INK4a / ARF locus on chromosome 9p21 encodes two gene products that are involved in cell cycle regulation through inhibition of CDK 4 mediated RB phosphorylation ( p16INK4a ) and binding to MDM 2 leading to p 53 stabilization ( p14ARF ) . ^^^ |
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| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Immunohistochemical expression of the p 53 , mdm 2 , p21 / Waf 1 , Rb , p 16 , Ki 67 , cyclin D 1 , cyclin A and cyclin B 1 proteins and apoptotic index in T cell lymphomas . ^^^ Fifty seven cases of T cell lymphomas ( TCL ) including 5 lymphoblastic ( T LBL ) and 52 peripheral TCL ( PTCL ) were analyzed by immunohistochemistry for the expression of p 53 , mdm 2 , p 21 , Rb , cyclin D 1 , cyclin A , cyclin B 1 , and Ki67 / MIB1 proteins and 39 / 52 PTCL were also analyzed for the expression of p 16 protein and for the presence of apoptotic cells by the TUNEL method . ^^^ |
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| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| The clinicopathological features and importance of p 53 , Rb , and mdm 2 expression in phaeochromocytomas and paragangliomas . ^^^ The aims of this study were to analyse the characteristics and the possible roles of p 53 , Rb , and mdm 2 alterations in these tumours . ^^^ The tumours were studied for the expression of p 53 , Rb , and mdm 2 by immunohistochemical methods . ^^^ Paragangliomas were often small , non functional , and presented incidentally , whereas phaeochromocytomas were usually large , functional , and symptomatic . p 53 overexpression , loss of Rb expression , and mdm 2 overexpression were seen in four , 43 , and 37 of the patients , respectively . ^^^ Loss of Rb expression was often found in phaeochromocytomas , whereas mdm 2 overexpression was more frequently seen in paragangliomas . ^^^ |
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| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Disruptions of the p 16 CDK4 / cyclin D 1 pRb pathway ( RB pathway ) and the p14ARF MDM 2 p53 pathway ( p 53 pathway ) are important mechanisms in the development of human malignancies . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| INK4a is a prognostic marker in resected ductal pancreatic cancer : an analysis of p16INK4a , p 53 , MDM 2 , an Rb . ^^^ OBJECTIVE : To identify the prognostic relevance of the G1 / S cell cycle regulator genes p16INK4a , p 53 , MDM 2 , and Rb in patients with resected ductal pancreatic cancer ( PC ) . ^^^ Protein expression of p 16 , p 53 , MDM 2 , and Rb was investigated , and mutation analysis of p16INK4a and p 53 was performed . p16INK4a promoter hypermethylation was examined by methylation specific polymerase chain reaction . ^^^ Stabilization of MDM 2 and loss of Rb expression were identified in a minority of tumors , independent of survival length . ^^^ CONCLUSIONS : The presence of p16INK4a alterations in resected tumors of patients with PC is connected with a worse prognosis , indicating patients that might benefit from adjuvant therapy regimens . p 53 alterations , MDM 2 overexpression , and loss of Rb expression could not be identified as prognostic markers from this study , but a larger study with greater statistical power might show a different result with regard to p53 . . ^^^ |
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| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| To elucidate the role of p53 / p16 ( INK4a ) / RB1 pathways in prostate carcinogenesis , we analyzed the p 14 ( ARF ) , p 16 ( INK4a ) , RB 1 , p 21 ( Waf 1 ) , p 27 ( Kip 1 ) , PTEN , p 73 , p 53 , and MDM 2 gene status of multiple areas within 16 histologically heterogeneous prostate carcinomas using methylation specific polymerase chain reaction , differential polymerase chain reaction , and immunohistochemistry . ^^^ |
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| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Mdm 2 can also bind to other cellular proteins such as hNumb , E2F1 , Rb and Akt ; however , the biological significance of these interactions is less clear . ^^^ |
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| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Using a set of cell lines with differing p 53 and Rb status we determined that Mdm 2 induction of E2F1 transactivation was p 53 dependent , resulting from release of repression by p 53 . ^^^ Furthermore , E2F1 repression by an Rb phosphorylation mutant could not be reversed by Mdm 2 overexpression . ^^^ |
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| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| By sequestering mdm 2 , ARF may promote growth suppression through the Rb pathway as well because mdm 2 can bind to Rb and attenuate its function . ^^^ Nevertheless , Ad1beta suppresses tumor cell growth and viability in vitro and in vivo , inducing G 1 or G 2 cell cycle arrest and cell death even in tumor cells lacking both functional Rb and p 53 pathways , and independently of induction of the p 53 downstream targets , p 21 , bax , and mdm 2 . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| FISH analysis was performed on the oncogene cyclin D 1 and IHC analysis on the proteins cyclin D 1 , p 53 , p 16 , cdk 4 , bcl 2 , mdm 2 and rb . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Our previous and these results suggested that the expression level of MDM 2 protein is independent of the status of the p14ARF , p 53 , Rb genes . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Furthermore , the expression of cell cycle related gene products TGF beta receptor 1 and 2 , MDM 2 , p 53 , p 21 , p 16 , Rb , and Bcl 2 were studied . ^^^ In more than 90 % ( n > / =27 ) of these cases , expression of TGFbeta receptor 1 and 2 , MDM 2 , p 53 , p 21 , p 16 , Rb , and Bcl 2 was detected in lesional LCH cells . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| The genes associated with bladder carcinogenesis include oncogenes ( such as H ras , FGFR 3 , erbB 2 , CCND 1 , mdm 2 ) , tumor suppressor genes ( such as INK4A / ARF , Rb , TP 53 , PTEN , TSC 1 , PTCH , DBCCR 1 ) , and DNA mismatch repair genes , etc . ^^^ |
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| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND AND OBJECTIVES : Disruption of either the p14ARF mdm 2 p 53 or p16INK4A Rb 1 pathways produces a breakdown of regulatory mechanisms and creates a gateway for tumorigenesis . ^^^ |
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| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| MDM 2 also interacts with the retinoblastoma protein ( RB ) and the transcription factor E2F1 to promote cell cycle S phase entry . ^^^ |
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| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| They expressed vimentin , HNK 1 , HBA 71 , Ki 67 , cyclin D 1 , Fli 1 , S 100 , p 21 , p 27 , and p 53 and were negative for cytokeratin , EMA , p 14 , p 16 , MDM 2 , Rb , and c erb b 2 antigens . ^^^ |
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| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| In our study , we screened HCCs resulting from HCV infection ( 51 cases ) , HBV infection ( 26 cases ) or excess alcohol intake ( 23 cases ) for alterations in genes involved in the RB 1 pathway ( p 16 ( INK4a ) , p 15 ( INK4b ) , RB 1 , CDK 4 and cyclin D 1 ) , the p 53 pathway ( p 53 , p 14 ( ARF ) and MDM 2 ) and the Wnt pathway ( beta catenin , APC ) . ^^^ |
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| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| PURPOSE : The growth suppressive genes p16INK4a and p14ARF located on the 9p21 gene cluster have active roles in the Rb and p 53 growth control pathways , respectively . p16INK4a is a cyclin dependent kinase inhibitor functioning upstream of Rb . p14ARF restrains cell growth by abrogating Mdm 2 inhibition of p 53 activity , thereby , facilitating p 53 mediated cell cycle arrest and apoptosis . ^^^ |
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| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| These included 3 genes coding for proteins in the p 53 pathway ( i . e . , TP 53 , p 14 ( ARF ) , and MDM 2 ) , 4 in the Rb 1 pathway ( i . e . , CDKN2A , CDKN2B , RB 1 , and CDK 4 ) , as well as PTEN and epidermal growth factor receptor . ^^^ |
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| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Consequently , MDM 2 suppressed p21waf1 / cip1 induced cell growth arrest of human p 53 ( / ) and p 53 ( / ) / Rb ( / ) cells . ^^^ |
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| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Clinical factors included FIGO stage , age , histopathologic type , and protein expression of p 53 , Rb , MDM 2 , p14ARF , p21WAF ( 1 ) / CIP ( 1 ) was determined by an immunohistochemical technique . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| RESULTS : We found that p 53 , p 21 , Rb , cyclin D 1 ( CCD 1 ) , MDM 2 , and gamma catenin were positive in 81 , 60 , 70 , 31 , 88 , and 44 % of patients , respectively . ^^^ |
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| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Our results demonstrated that 16 genes ; bad , bax , bcl 2 , bcl w , bcl 10 , caspase 3 , caspase 7 , caspase 8 , c myc , E 124 , GADD 45 , mdm 2 , NKkappab 1 , p 53 , p 21 , Rb and trail were up regulated and six genes ; caspase 1 , caspase 2 , DR 5 , E2F1 , FasL and iNOS did not changed in response to DES treatment in wild type mice compared to p53+ / knockout mice . ^^^ |
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| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| In this study , we found that Mdm 2 , a ubiquitin ligase for p 53 , promoted ubiquitin dependent degradation of pRB . pRB was efficiently ubiquitinated by wild type Mdm 2 in vivo as well as in vitro , but other RB family proteins were not . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Levels of MDM 2 , a negative regulator of p 53 , and the hypophosphorylated form of RB were also elevated , consistent with the triggering of G 1 cell cycle checkpoint . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| As combinations of genetic and / or epigenetic alterations occurring during salivary gland carcinogenesis are largely unknown , we here analyzed 36 salivary gland carcinomas ( SGCs ) for changes in INK4a / ARF , RB 1 , p 21 , p 27 , PTEN , p 53 , MDM 2 and O 6 MGMT genes using methylation specific PCR ( MSP ) , loss of heterozygosity ( LOH ) assays and mutational analysis with immunohistochemistry ( IHC ) , as well as histone H 3 and H 4 acetylation status . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Studying the influence of phosphorylation of mdm 2 by CK 2 upon interaction with some of these binding partners , we found that the retinoblastoma ( Rb ) protein bound more strongly to the unphosphorylated mdm 2 than to its CK 2 phosphorylated counterpart . ^^^ Cells transfected with the S269D mutant exhibited a different growth behavior than wild type mdm 2 expressing cells , which might be attributed to the altered Rb mdm 2 interaction . . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Low grade superficial papillary tumors induced by activated H ras had no detectable Rb family proteins ( Rb , p 107 , and p 130 ) and late cell cycle cyclins and kinases ( cyclin A , E , and CDK 1 ) , but had increased level of p 16 , p 53 , and MDM 2 . ^^^ |
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| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Here we analyzed various tumor regions with features of low grade and high grade astrocytomas from 9 autopsy proven GC cases for the immunoexpression of the cell cycle controlling proteins mdm 2 , p 21 , p27 / kip1 , p 16 , and Rb . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| These included three genes coding for proteins in the p 53 pathway ( TP 53 , p 14 ( ARF ) and MDM 2 ) , four in the Rb 1 pathway ( CDKN2A , CDKN2B , RB 1 and CDK 4 ) and PTEN and EGFR . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| EXPERIMENTAL DESIGN : We determined the mRNA expression of p 1 ( INK4A ) , p 14 ( ARF ) , CDK 4 , RB 1 , MDM 2 , TP 53 , and E2F1 by quantitative reverse transcription PCR in 38 cases of GISTs and correlated the findings with clinicopathologic factors , including mutation analysis of KIT and PDGFRA . ^^^ GISTs with low mRNA expression of the CDKN2A transcripts p 16 ( INK4A ) and p 14 ( ARF ) but high mRNA expression of CDK 4 , RB 1 , MDM 2 , TP 53 , and E2F1 were associated with aggressive clinical behavior and unfavorable prognosis , whereas GISTs with a low mRNA expression of CDK 4 , RB 1 , MDM 2 , TP 53 , and E2F1 were not . ^^^ |
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| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| In this study , we find that MDM 2 promotes Rb degradation in a proteasome dependent and ubiquitin independent manner . ^^^ We show that Rb , MDM 2 , and the C 8 subunit of the 20S proteasome interact in vitro and in vivo and that MDM 2 promotes Rb C 8 interaction . ^^^ Expression of wild type MDM 2 , but not the mutant MDM 2 defective either in Rb interaction or in RING finger domain , promotes cell cycle S phase entry independent of p 53 . ^^^ Furthermore , MDM 2 ablation results in Rb accumulation and inhibition of DNA synthesis . ^^^ Taken together , these findings demonstrate that MDM 2 is a critical negative regulator for Rb and suggest that MDM 2 overexpression contributes to cancer development by destabilizing Rb . . ^^^ |
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| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| These findings suggest that overexpression of Mdm 2 can perturb a RB pathway regardless of the p 53 gene status , promoting carcinogenesis . . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| We demonstrate cyclic resveratrol mediated expression of p 53 , mdm 2 , p 21 ( cip / waf ) , Rb , and cyclin G at both the RNA and the protein level at < 8 h . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Analysis of mRNA and protein levels in melanomas with 12q14 amplification demonstrated overexpression of target genes CDK 4 and MDM 2 without loss of CDKN2A P 16 ( P16INK4A ) or CDKN2A P14ARF ( P14ARF ) expression , important regulators of the RB 1 and TP 53 pathways , which are commonly lost or mutated in melanoma . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Immunohistochemical stains were carried out on tissue microarrays to evaluate the expression of proteins involved in the G ( 1 ) S transition and proteins that regulate apoptosis including Rb , E2F1 , cyclin D 1 , CDK 4 , CDK 6 , p 27 ( KIP 1 ) , p 21 ( WAF1 / CIP1 ) , p 53 , Mdm 2 , Bcl 2 , and Bax . ^^^ The positive phenotypes identified were as follows : Rb , 39 . 1 % ; E2F1 , 69 . 6 % ; cyclin D 1 , 30 . 4 % ; CDK 4 , 100 % ; CDK 6 , 30 . 4 % ; 39 . 1 % ; p 27 ( KIP 1 ) , 47 . 8 % ; p 21 ( WAF1 / CIP1 ) , 39 . 1 % ; p 53 , 43 . 5 % ; Mdm 2 , 17 . 4 % ; Bcl 2 , 91 . 3 % ; and Bax , 100 % . ^^^ |
|
| Interacting proteins: Q00987 and P06400 |
Pubmed |
SVM Score :0.0 |
| Oncogenes ( H ras , erbB 2 , EGFR , MDM 2 , C MYC , CCND 1 ) , tumor suppressor genes ( p 53 , Rb , p 21 , p27 / KIP1 , p 16 , PTEN , STK 15 , FHIT , FEZ1 / LZTS1 , bc 10 ) , telomerase , and methylation have all been studied in bladder cancer . ^^^ |
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