| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| A consensus caspase cleavage site at the C terminus of RB is cleaved in vitro and in vivo by proteases related to CPP 32 ( caspase 3 ) . ^^^ |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| Apoptosis was also associated with poly ( ADP ribose ) polymerase ( PARP ) cleavage , thus indicating activation of CPP32 / Yama / apopain ( caspase 3 ) ; however , while the tripeptide caspase inhibitor Z Val Ala DL Asp fluoromethylketone was able to prevent both C 6 Cer induced PARP cleavage and apoptosis , it was ineffective in preventing RB dephosphorylation . ^^^ |
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| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| Tumor necrosis factor or staurosporine induced apoptosis of caspase 3 deficient MCF 7 cells resulted in cleavage of the death substrates PARP , Rb , PAK 2 , DNA PKcs , gelsolin , and DFF 45 , but not alpha fodrin . ^^^ Together our results suggest that caspase 3 is essential for cleavage of alpha fodrin , but dispensable for the cleavage of PARP , Rb , PAK 2 , DNA PKcs , gelsolin , and DFF 45 and imply that one or more caspases other than caspases 2 , 3 , and 7 is activated and plays a crucial role in the cleavage of these substrates in MCF 7 cells . . ^^^ |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| We also show that the carboxy terminal cleavage of RB is detected immediately following caspase 3 and PARP cleavage during FAS mediated apoptosis of MCF 10 cells . ^^^ |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| The data indicate that the caspase 3 activation and cleavage of Rb protein may represent important steps in the regulation pathway of bax mediated spontaneous apoptosis . ^^^ |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| The cytosol induced Rb proteolysis in vitro , which was inhibited by a caspase 3 inhibitor . ^^^ These findings indicate that caspase 3 induces DNA fragmentation and cleavage of Rb and PARP during the process of apoptosis induced by concanamycin A . . ^^^ |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| CPP 32 specific cleavage of the RB protein was also detected and enhanced by the addition of DBcAMP in a dose dependent manner . ^^^ |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| Caspase 3 like activity increases following K+ deprivation and the time course correlates with RB cleavage and apoptosis . ^^^ Although the use of a specific caspase 3 like inhibitor ( z DEBD . fmk ) delays RB cleavage and reduces DNA fragmentation , data implicate other caspases in these processes . ^^^ However , K+ deprivation induces a gradual production of the active p 20 subunit of caspase 3 ( CPP 32 ) that coincides with RB disappearance at the cellular level . ^^^ |
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| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| Collectively , a model for the PDT triggered apoptotic signalling cascade with RB is proposed , which involves singlet oxygen , JNK , caspase 3 and PAK 2 , sequentially . . ^^^ |
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| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| We also found coinduction of several groups of related genes that might represent functional modules within the ischemic neuronal transcriptome , including VEGF and its receptor , NRP 1 ; the IGF 1 receptor and the IGF 1 binding protein IGFBP 2 ; Rb , the Rb binding protein E2F1 , and the E2F related transcription factor , TFDP 1 ; the CACNB 3 and CACNB 4 beta subunits of the voltage gated calcium channel ; and caspase 3 and its substrates , ACINUS , FEM 1 , and GSN . ^^^ |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| The anti tumor effects were independent of the genomic status of p 53 , RB , p 16 , ras , bax , and caspase 3 in these cells . ^^^ |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| Therefore , we studied the cell cycle proteins , Rb , E2F2 , Ki 67 , p 21 and p 53 and the apoptotic proteins , mcl 1 , hax , bcl xl , bcl 2 , Fas receptor , Fas ligand , caspase 3 , M 30 and PARP as potential predictive factors . ^^^ |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| In view of the pivotal role of caspase 3 in the regulation of neuronal apoptosis during development , we examined its function in the execution of the wide spread neuronal cell death induced by Rb deficiency . ^^^ Second , caspase 3 deficiency does not extend the life span of Rb null embryos , because double null mutants exhibit high rates of liver apoptosis resulting in erythropoietic failure . ^^^ Third , Rb / caspase 3 double mutant neurons of the CNS exhibit widespread apoptosis similar to that seen in Rb mutants alone ; thus caspase 3 deficiency does not protect this population from apoptosis . ^^^ Finally , in contrast to the CNS , neurons of the PNS including those comprising the trigeminal ganglia and the dorsal root ganglia are protected from apoptosis in Rb / caspase 3 double mutant embryos . ^^^ |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| Activation of caspase 3 and cleavage of Rb are associated with p 16 mediated apoptosis in human non small cell lung cancer cells . ^^^ The retinoblastoma ( Rb ) cell cycle regulator protein was also cleaved after activation of caspase 3 ; when the levels of Rb significantly diminished , apoptosis began . ^^^ These data implied that cleavage of Rb , in addition to activation of caspase 3 , represented a mechanism by which Adv / p16 induced apoptotic cell death in human NSCLC cells . ^^^ |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| JTE 522 inhibited the expressions of COX 2 mRNA , phosphorylated Rb , and CDK 4 proteins , while increased the levels of p 53 , p 21 , cyclin D 1 proteins , and the activity of caspase 3 in RL 95 2 cells . ^^^ CONCLUSION : JTE 522 inhibits cell proliferation and induces apoptosis in RL 95 2 cells , which may be associated with the activation of caspase 3 like proteases , down regulation of the expression of COX 2 mRNA , phosphorylated Rb , and CDK 4 proteins , and up regulation of the expressions of p 53 , p 21 , and cyclin D 1 proteins . . ^^^ |
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| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| Correspondingly , the release of cytochrome c and the activation of caspase 3 induced by TNFRI , but not by DNA damage , are defective in cells expressing Rb MI . ^^^ |
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| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| Our results demonstrated that 16 genes ; bad , bax , bcl 2 , bcl w , bcl 10 , caspase 3 , caspase 7 , caspase 8 , c myc , E 124 , GADD 45 , mdm 2 , NKkappab 1 , p 53 , p 21 , Rb and trail were up regulated and six genes ; caspase 1 , caspase 2 , DR 5 , E2F1 , FasL and iNOS did not changed in response to DES treatment in wild type mice compared to p53+ / knockout mice . ^^^ |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| The results show that upon induction of LMP 1 expression , the expression of Survivin in nucleus , level of phosphorylated retinoblastoma gene ( Rb ) , the number of cells in S stage of cell cycle , and the cell colony formation rate were higher than those without LMP 1 induction ; if the expression of Survivin and the nucleus translocation of Survivin were knocked by introduction of anti sense PS ODN Survivin and anti Survivin antibodies respectively , apoptosis rates and the activity of caspase 3 increased . ^^^ |
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| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| Immunohistochemistry was performed for cell cycle regulators [ p 53 , p 21 , p 27 , p 16 , cyclin D 1 , Rb ] , apoptosis related proteins [ Fas , Fas L , FADD , TRAIL , DR 4 , DR 5 , caspase 8 , caspase 3 , bcl 2 , Bax ] , tumor suppressor proteins [ beta catenin , E cadherin , FHIT , Smad 4 , VHL , PTEN , KAI 1 ] , and oncoproteins [ c myc , COX 2 , EGFR ] . ^^^ |
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| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| Histone acetylation status and the expression of p21WAF1 , phosphorylated retinoblastoma protein ( Rb ) , poly ( adenosine diphosphate ribose ) polymerase ( PARP ) , and caspase 3 were studied using Western blot analysis . ^^^ |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| Attempted cell cycle induction in post mitotic neurons occurs in early and late apoptotic programs through Rb , E2F1 , and caspase 3 . ^^^ Furthermore , maintenance of the integrity of the Rb protein is specifically dependent upon caspase 3 like activity , since caspase 3 can cleave Rb during free radical activity and this degradation of Rb can be blocked during the inhibition of caspase 3 activity . ^^^ |
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| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| The Rb family members p 107 and p 130 were not deregulated in cells in which Rb had been deleted , as determined by quantitative RT PCR on the highly enriched stem and primitive progenitor cell lin ( ) c Kit ( + ) Sca 1 ( + ) population . ^^^ |
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| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| Inhibition of survivin by RNA interference reduced cell proliferation and induced apoptosis in Ishikawa cells by down regulating cyclin D 1 and phosphorylated RB and activating caspase 3 and caspase 8 . ^^^ |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| Here we show that in HCT 116 colon carcinoma cells , 10 mM sodium salicylate induces caspase 3 activation and degradation of its substrates , poly ( ADP ribose ) polymerase ( PARP ) , beta catenin , and retinoblastoma ( Rb ) . ^^^ |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| Furthermore , the expression of some cell cycle and apoptosis related proteins , such as Bcl 2 , Rb , P 16 , caspase 3 , cyclin A and cyclin E , was altered in As ( 2 ) O ( 3 ) treated NB 4 cells . ^^^ |
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| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| The increase in caspase 3 activity after coincubation with statins and staurosporine was paralleled by an increase in the protein level of the pro apoptotic GTPase RhoB . ^^^ |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| Apoptosis was evaluated by TUNEL technique ( TdT mediated dUTP nick end labeling ) and the presence of EBV latent membrane protein 1 as well as expression of Bcl 2 , tumor suppressor p 53 , p21WAF1 , MDM 2 , Rb 1 , PCNA , p27KIP1 and caspase 3 , was detected immunocytochemically on paraffin embedded lymph node specimens obtained at diagnosis . ^^^ |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06400 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|