| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| There was a decrease in Rb phosphorylation but without the induction of WAF 1 protein . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| The elevated levels of p 21 ( waf 1 ) protein led to decreased Rb phosphorylation and cdk 2 activity . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| Unlike p 53 , p 16 , and Rb , mutations in Kip 1 and WAF 1 genes are distinctly rare in bladder cancer . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| On the basis of Western immunoblot analysis of Rb , p 53 and cell cycle inhibitory protein ( Cip 1 ) ( p 21 ) proteins , TNF alpha decreased Rb protein expression in a dose and time dependent manner whereas it increased the expression level of tumor suppressor p 53 protein . ^^^ Taken together these results suggest that Rb , p 53 , and Cip 1 ( p 21 ) proteins mediate TNF alpha antimitogenic activity , and TNF alpha induces growth arrest in the G 1 phase in MCF 7 cells . . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| CIP 1 encodes a novel 21 kd protein that is found in cyclin A , cyclin D 1 , cyclin E , and Cdk 2 immunoprecipitates . p21CIP1 is a potent , tight binding inhibitor of Cdks and can inhibit the phosphorylation of Rb by cyclin A Cdk 2 , cyclin E Cdk 2 , cyclin D 1 Cdk4 , and cyclin D 2 Cdk4 complexes . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| Analysis of these cells revealed a decrease in the expression of p 21 ( WAF 1 , sdi 1 , or CIP 1 ) and retinoblastoma ( Rb ) gene product . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| Investigating the cell cycle mechanisms involved in growth inhibition , we found that carbachol treatment decreased cyclin D 1 levels , increased p 21 ( cip 1 ) expression , and led to hypophosphorylation of the retinoblastoma gene product ( Rb ) . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| In this review , we describe three senescence inducing pathways involving these inhibitors , namely , the p 16 ( INK4a ) / Rb pathway , the p 19 ( ARF ) / p53 / p21 ( Cip 1 ) pathway , and the PTEN / p27 ( Kip 1 ) pathway . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| CDP / cut is cell cycle regulated through interactions with Rb , p 107 , specific kinases and phosphatases directing the transcriptional activity of CDP / cut on such genes encoding p 21 ( WAF 1 , CIP 1 ) , c myc , thymidine kinase , and histones . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| We have analyzed the expression of the CDKN1A ( p 21 ( CIP 1 ) ) , CDKN1B ( p 27 ( Kip 1 ) ) , TP 53 , RB 1 and MDM 2 proteins and tumor cell proliferation by immunohistochemical staining in 59 cases of metastatic melanoma . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| This S phase dephosphorylation of RB and subsequent inhibition of DNA replication was observed in p 21 ( Cip 1 ) deficient cells . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| Genomewide analysis of RAS induced p 53 mutant melanomas by comparative genomic hybridization and candidate gene surveys revealed alterations of key components governing RB regulated G ( 1 ) / S transition , including c Myc , cyclin D 1 , cdc25a , and p 21 ( CIP 1 ) . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| Activated caspase 3 is targeted to cleave p 21 ( cip 1 ) , p 27 ( kip 1 ) , and Rb , which play important roles in TGF beta induced G 1 arrest , into inactive fragments . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| To examine the association of cell cycle regulatory gene inactivation with human cell immortalization , we determined the expression status of INK4a , Rb , and WAF1 / CIP 1 , in eleven in vitro immortalized human cell lines , including fibroblasts and keratinocytes . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| Western blot analysis of the cell extracts prepared at regular intervals following release from isoleucine block revealed remarkable differences in the expression of cyclin E , p 21 ( Cip 1 ) , p 27 ( Kip 1 ) , and Rb at the protein level between LNCaP and DU 145 cells during progression from G ( 1 ) into S phase . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| In rasREF cells treated with DE for 72 h in suspension culture ( a ) , the levels of cyclin D 1 , cyclin A , p 27 ( Kip 1 ) , and hyperphosphorylated Rb were decreased , but the levels of cdk 4 , cdk 6 , cdk 2 , p 16 ( INK4a ) , and p 21 ( Cip 1 ) were not affected . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| Furthermore , the existence of a correlation between this effect and the expression of the cyclin kinase inhibitor p 21 ( CIP 1 ) and retinoblastoma ( Rb ) protein was also examined . ^^^ Protein expressions of p 21 ( CIP 1 ) and Rb protein were determined by Western blot analysis . ^^^ In conclusion , ibuprofen inhibits tendon cell proliferation in a process that is probably mediated by the upregulation of p 21 ( CIP 1 ) and reduced phosphorylation of Rb protein . . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| OSI 461 enhanced the G 0 G1 arrest caused by acyclic retinoid , and the combination of these agents caused a synergistic decrease in the levels of expression of cyclin D 1 protein and mRNA , inhibited cyclin D 1 promoter activity , decreased the level of hyperphosphorylated forms of the Rb protein , induced increased cellular levels of the p 21 ( CIP 1 ) protein and mRNA , and stimulated p 21 ( CIP 1 ) promoter activity . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| All tumor samples expressed normal sized RB 1 , cyclin D 3 , CDK 2 , CDK 4 , p 21 ( CIP 1 ) , and p 27 ( KlP 1 ) proteins , and only a single tumor showed an aberrant protein band for one of these proteins , p 21 ( CIP 1 ) . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| It is shown that RA reduces RB expression and enhances RB phosphorylation by a mechanism that involves down regulation of the cyclin dependent kinase inhibitor ( CKI ) p 21 ( Cip 1 ) , having this fact as important consequences for both the cell cycle progression and the adipocyte differentiation process . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| Rosiglitazone induced growth inhibition was associated with cell cycle arrest and changes in cell cycle regulators , such as an increase of cyclin dependent kinases inhibitors p 21 ( cip 1 ) and p 27 ( kip 1 ) , a decrease of cyclin D 1 , and inactivation of Rb protein . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| Twenty primary central nervous system lymphomas ( PCNSL ) from immunocompetent patients ( nineteen B cell lymphomas and one T cell lymphoma ) were investigated for genetic alterations and / or expression of the genes BCL 2 , CCND 1 , CDK 4 , CDKN1A , CDKN2A , MDM 2 , MYC , RB 1 , REL , and TP 53 . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| Cell growth regulators include proteins of the p 53 pathway encoded by the genes CDKN2A ( p 16 , p14arf ) , MDM 2 , TP 53 , and CDKN1A ( p 21 ) as well as proteins encoded by genes like RB 1 , E2F , and MYCL . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| To elucidate the biochemical processes involved in the pathogenesis of B CLL and MCL , we analyzed the expression level of a set of genes that play central roles in apoptotic or cell proliferation pathways and of candidate genes from frequently altered genomic regions , namely ATM , BAX , BCL 2 , CCND 1 , CCND 3 , CDK 2 , CDK 4 , CDKN1A , CDKN1B , E2F1 , ETV 5 , MYC , RB 1 , SELL , TFDP 2 , TNFSF 10 , and TP 53 . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| These include genes that may act through chromatin remodelling and / or as transcription factors / regulators ( Cdkn1a , Cdr 2 , Hrmt1l2 , Idb 2 , Myc / c myc , L myc 1 , Rb 1 , Sap 30 and Tgif ) , genes possibly involved in the regulation of muscle membrane properties and / or excitation contraction coupling ( Cacng 1 , Camk2d , Hrmt1l2 , Kcnj 12 , Kcna 7 and Rrad ) and genes potentially involved in neuromuscular interactions and / or receptor signalling ( Acvr2b , Adam 19 , D0H4S114 , Kai 1 , Maged 1 , Mt 2 , Prkcabp , Ptp4a3 , Ramp 1 , Rras , Timp 1 , Vegfa and Zfp 145 ) . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| MATERIALS AND METHODS : An inverse polymerase chain reaction ( PCR ) based methodology was used to amplify rearrangements that had occurred between one of four target regions ( in or neighbouring the avian myelocytomatosis viral oncogene homologue ( c MYC ) , cyclin dependent kinase inhibitor 1A ( CDKN1A ) , fibroblast growth factor receptor 2 ( FGFR 2 ) , or retinoblastoma 1 ( RB 1 ) genes ) and sequences elsewhere in the genome , following gamma irradiation and subsequent incubation at 37 degrees C of normal human IMR 90 fibroblasts . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| Furthermore , both hyperphosphorylated and hypophosphorylated forms of Rb were detected at 48 h , but only the hypophosphorylated form was detected at 120 h of treatment . p21Cip1 , which was very abundant in the ventral prostate of castrated and intact rats , was not detected when the prostate started proliferation and increased gradually as proliferation decreased during the androgen treatment . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| The accelerated and more dramatic induction of differentiation seen in the p21Cip1 overexpressors was associated with a more rapid reduction of CDK 2 kinase associated activity , increased levels and more rapid dephosphorylation of the Rb protein , and increased levels of the cyclin D 3 protein . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| By contrast , differentiated myocytes with RB ( N757F ) undergo DNA synthesis and phosphorylate RB ( N757F ) in response to serum , despite a high level of p21Cip1 expression . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| In SMC , platelet derived growth factor ( 5 ng / ml ) enhanced 3H thymidine incorporation ( 298 + / 23 % , n = 4 ) , activated cyclin dependent kinase ( Cdk 2 ) , phosphorylated Rb and down regulated p27Kip1 ( but not p21CiP1 ) . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| Loss of E2F function results in an elevation of p21Cip1 protein , leading to a decrease in cyclin dependent kinase activity and Rb phosphorylation . ^^^ These findings suggest a function for this subclass of E2F transcriptional activators in a positive feedback loop , through down modulation of p21Cip1 , that leads to the inactivation of Rb dependent repression and S phase entry . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| Reduced phosphorylation of the retinoblastoma protein ( Rb ) and CDK 2 activity , increased expression of p21Cip1 and enhanced binding of p21Cip1 to CDK 2 were observed following treatment with PA . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| Identification and characterization of their substrates indicate that they regulate the turnover of key cell cycle proteins ( p27Kip1 , p21Cip1 , p57Kip2 , cyclin E ) , tumor suppressor proteins ( p 53 , RB ) , signaling kinases ( Src , Zap 70 , PI 3 kinase ) , apoptosis regulators ( Bcl 2 , Bax , Bik ) and transcription factors ( Myc , NF kappaB , E1F1 ) , all of which have been implicated in the pathogenesis of malignant lymphoma . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| Activated Notch inhibits mitogen induced upregulation of p21Cip1 and delays cyclin D cdk 4 mediated Rb phosphorylation . ^^^ Inhibition of the Notch pathway at confluence prevents p21Cip1 downregulation and induces Rb phosphorylation . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| AMP mediated inhibition of DNA replication and S phase progression : involvement of Rb , p21Cip1 , and PCNA . cAMP exerts an antiproliferative effect on a number of cell types including lymphocytes . ^^^ The cAMP induced inhibition of DNA synthesis was associated with the increased binding of p21Cip1 to Cdk 2 cyclin complexes , inhibition of Cdk 2 kinase activity , dephosphorylation of Rb , and dissociation of PCNA from chromatin in S phase cells . ^^^ The ability of cAMP to inhibit DNA replication and trigger release of PCNA from chromatin required Rb and p21Cip1 proteins , since both processes were only marginally affected by increased levels of cAMP in Rb / and p21Cip1 / 3T3 fibroblasts . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| Mitf cooperates with Rb 1 and activates p21Cip1 expression to regulate cell cycle progression . ^^^ The results indicate that Mitf mediated activation of p21Cip1 expression and consequent hypophosphorylation of Rb 1 will contribute to cell cycle exit and activation of the differentiation programme . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| The data demonstrate that SDI 1 is an important downstream effector of p 53 , but here we report that it can also cause inhibition of DNA synthesis in several immortal human cell lines , independent of p 53 or Rb status . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| We demonstrate here that the radiation signal transduction pathway operating through p 53 , its target gene WAF 1 , cyclin dependent kinases and the retinoblastoma ( Rb ) protein is defective in A T cells . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| In both LS 513 and LS 1034 , WAF 1 induction correlated with reduced cyclin E associated kinase activity in vitro and suppression of the retinoblastoma susceptibility gene ( Rb ) protein phosphorylation in vivo . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| Recently p 53 has been reported to stimulate the WAF 1 gene , and the WAF 1 protein to inhibit cdk activity , which inactivates the RB gene product by phosphorylation . ^^^ Therefore , reactivation of the WAF 1 or RB gene by certain drugs might compensate for the loss of function of p 53 in Li Fraumeni syndrome . ^^^ To find drugs regulating the promoters of these genes , it is reasonable to try G 1 arresting drugs , because WAF 1 and RB are thought to arrest cells at the G 1 phase . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| The expression of p 53 and WAF 1 ( a protein involved in p 53 mediated cell cycle arrest ) in human RB was determined by immunocytochemical analysis . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| WAF 1 are induced and Rb protein is hypophosphorylated during cell growth inhibition by the thymidylate synthase inhibitor ZD 1694 ( Tomudex ) . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| Immunocytochemical analysis demonstrated positive nuclear staining for WAF 1 , Rb protein , c myc , and p 53 both in the original and metastatic tumors . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| Transfection of U 937 tumor cells with p21WAF 1 resulted in expression of the HUMSIAH ( human seven in absentia homologue ) , Rb , and Rbr 2 genes and strong suppression of the malignant phenotype . p 21 ( WAF 1 ) drastically modified the compartmentalization of the nuclear genome . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| When A 431 cells were treated with EGF , a cascade of responses were observed , including immediate hyperphosphorylation of EGF receptor on tyrosine residues , accumulation of WAF 1 mRNA and p21 / WAF1 protein , dephosphorylation of RB protein which is a substrate of CDK cyclin , and cell cycle arrest . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| In a series of 126 patients with hepatocellular carcinoma ( HCC ) , we immunohistochemically evaluated tumor expression of the cell cycle related gene protein products of Rb , p 21 ( WAF 1 ) , and p 53 . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| The inhibition of G 2 progression was correlated with increased contents both of total p 21 ( Waf 1 ) and of p 21 ( Waf 1 ) associated with cyclin A and with an inhibition of cyclin A and B 1 associated histone H 1 kinase activities measured in cell lysates , as well as with dephosphorylation of the RB protein . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| Overexpression of p 21 ( waf 1 ) decreases G 2 M arrest and apoptosis induced by paclitaxel in human sarcoma cells lacking both p 53 and functional Rb protein . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| RhoB GG induced the cell cycle kinase inhibitor p 21 ( WAF 1 ) in a p 53 dependent manner , similar to FTI treatment , but this event was dispensable because RhoB GG could still inhibit the growth of p 53 null cells that lacked p21WAF1 activation . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| Several proto oncogenes and tumor suppressor genes have been implicated in the regulation of telomerase activity , both directly and indirectly ; these include c Myc , Bcl 2 , p 21 ( WAF 1 ) , Rb , p 53 , PKC , Akt / PKB , and protein phosphatase 2A . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| Overexpression of wild type waf 1 in melanoma cells reduced growth of subconfluent cells , decreased Cdk 4 activity with a concomitant increase in hypophosphorylated Rb , and promoted cell death by apoptosis . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| SIPS modified strongly the E 7 mediated regulatory control on p 107 , p 16 ( Ink 4a ) , p 19 ( Ink 4d ) , p 27 ( Kip 1 ) , p 21 ( Waf 1 ) and Rb mRNA level , when compared to normal conditions . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| Physiologic concentrations of genistein ( < or = 20 microM ) decreased LNCaP viable cell number in a dose dependent manner , induced a G ( 1 ) cell cycle block , decreased prostate specific antigen mRNA expression , and increased p 27 ( KIP 1 ) and p 21 ( WAF 1 ) ( mRNA and protein ) but had no effect on apoptosis or the mRNA expression of the apoptosis and cell cycle related markers bcl 2 , bax , Rb , and proliferating cell nuclear antigen . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| TSA enhanced the protein expression of p 21 ( WAF 1 ) , CREB binding protein , cyclinE , cyclin A , Bak and Bax , while it reduced the expression of E2F 1 , E2F 4 , HDAC 1 , p 53 and hyperphosphorylated form of Rb . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| In the attempt to elucidate whether this compound might affect genes playing a role in G1 / S phase transition , the expressions of p 53 , p 21 ( WAF 1 ) , cyclin D 1 and Rb , mainly involved in response to DNA damaging stress , were analyzed by Western blot . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| To elucidate the role of p53 / p16 ( INK4a ) / RB1 pathways in the tumorigenesis of primary central nervous system lymphomas ( PCNSLs ) , we have analyzed p 14 ( ARF ) , p 16 ( INK4a ) , RB 1 , p 21 ( Waf 1 ) , and p 27 ( Kip 1 ) status in a series of their 18 sporadic cases of diffuse large B cell lymphoma , using methylation specific PCR , differential PCR , and immunohistochemistry . ^^^ Hypermethylation of the RB 1 and the p 27 ( Kip 1 ) promoter region was detected in 2 ( 11 % ) cases , whereas p 21 ( Waf 1 ) methylation was not detected in any . ^^^ Hypermethylation of RB 1 , p 21 ( Waf 1 ) , and p 27 ( Kip 1 ) appears to be of minor significance , these genes being independently methylated in PCNSLs . . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| METHODS : We performed Western blot analysis of five cell cycle stimulating ( cyclins D 1 , E , B 1 , cdk 2 , cdk 4 ) and three cell cycle inhibiting ( p 16 ( INK4a ) , p 21 ( WAF 1 ) , Rb ) proteins in 41 endometrial carcinoma specimens . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| To determine whether this difference can be translated into a therapeutic advantage to protect normal cells from adverse cytotoxicity caused by chemotherapy , we established cell model systems for ecdysone inducible expression of p 16 ( Ink4a ) , p 21 ( Waf 1 ) , and p 27 ( Kip 1 ) in one CKI responsive cell line ( A 431 human vulvar epidermoid carcinoma cells with functional Rb ) and one CKI unresponsive cell line ( SiHa human cervical cancer cells with nonfunctional Rb ) . ^^^ However , only in A 431 cells did expression of p 16 ( Ink4a ) , p 21 ( Waf 1 ) , or p 27 ( Kip 1 ) cause Rb dephosphorylation , arrest cell cycle traversal , and potently inhibit cell proliferation . ^^^ Induction of p 16 ( Ink4a ) , p 21 ( Waf 1 ) , or p 27 ( Kip 1 ) in SiHa cells failed to cause Rb dephosphorylation or to arrest cell cycle traversal , and such induction only minimally inhibited cell proliferation . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| We have tested the effects of sodium butyrate and trichostatin A on human myeloma cell lines and have observed G 1 arrest and apoptosis associated with increased expression of p 21 ( WAF 1 ) , Bax , Rb dephosphorylation , and decreased IL 6 receptor ( IL 6R ) expression . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| By immunofluorescence and immunohistochemistry staining , the identify the expression of the inserted WAF 1 gene and its biological regulating effects on Rb , cyclin D 1 gene . ^^^ The functional characterization of the inserting WAF 1 gene implied that P21WAF1 / Cip1 expression could upregulate Rb gene and downregulate cyclin D 1 gene at protein level . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| To elucidate the role of p53 / p16 ( INK4a ) / RB1 pathways in prostate carcinogenesis , we analyzed the p 14 ( ARF ) , p 16 ( INK4a ) , RB 1 , p 21 ( Waf 1 ) , p 27 ( Kip 1 ) , PTEN , p 73 , p 53 , and MDM 2 gene status of multiple areas within 16 histologically heterogeneous prostate carcinomas using methylation specific polymerase chain reaction , differential polymerase chain reaction , and immunohistochemistry . ^^^ Focal hypermethylation of RB 1 , p 21 ( Waf 1 ) , and p 27 ( Kip 1 ) was detected within two , two , and three tumors , respectively . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| We found that Id 1 expression induced phosphorylation of RB and down regulation of p 16 ( INK4a ) but not p 21 ( Waf 1 ) or p 27 ( Kip 1 ) . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| To investigate the role of the cell cycle regulators p 21 ( Waf 1 ) , p 27 ( Kip 1 ) , retinoblastoma ( Rb ) , and cyclin D 1 in Richter ' s transformation of chronic lymphocytic leukemia ( CLL ) , we analyzed 19 CLL and eight Richter ' s syndrome ( RS ) tumors , previously characterized for p 53 and ARF / INK4a abnormalities . p 21 ( Waf 1 ) immunohistochemical expression was negative in 12 of 15 CLL ( 80 % ) , whereas it was moderate or strong in three of seven RS ( 43 % ) . p 21 ( Waf 1 ) gene was in germline configuration in all the tumors analyzed . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| Oxidized low density lipoprotein induces the cyclin dependent kinase inhibitor p 21 ( waf 1 ) and the tumor suppressor Rb . ^^^ In the present work , we demonstrate that OxLDL increased intracellular levels of the kinase inhibitor p 21 ( waf 1 ) ( p 21 ) and of the tumor suppressor Rb . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| RESULTS : We detected a significant reduction in the expression levels of the cell cycle related proteins p 21 ( waf 1 ) ( P=0 . 002 ) , p 27 ( kip 1 ) ( P=0 . 03 ) , Rb ( P=0 . 00002 ) , and L myc ( P=0 . 00000007 ) in muscle invasive tumors compared to noninvasive tumors . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| These data : ( 1 ) point to a back up ' senescence ' mechanism distinct from induction of p 21 ( Waf 1 ) or p 16 ( Ink4a ) ; and ( 2 ) provide an in vitro model of clonal evolution through successive dysfunction of Rb and p 53 pathways in a relevant human cell context . . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| Expression and significance of p 53 , rb , p21 / waf 1 , p16 / ink 4a , and PTEN tumor suppressors in canine melanoma . ^^^ The genes encoding the tumor suppressors p 53 , Rb , p 21 ( waf 1 ) , p 16 ( ink 4a ) , and PTEN have been postulated to contribute to the pathogenesis of melanoma in humans and experimental animal models . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| Of those genes examined , p 16 ( INK4a ) , O ( 6 ) MGMT , and GST P were found to be the most frequently methylated ( 66 % , 25 % and 75 % of cases , respectively ) , while methylations of p 14 ( ARF ) , RB 1 , p 21 ( Waf 1 ) , and p 27 ( Kip 1 ) were far less common ( 3 % , 6 % , 6 % and 6 % of cases , respectively ) . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| MyoD induces apoptosis in the absence of RB function through a p 21 ( WAF 1 ) dependent re localization of cyclin / cdk complexes to the nucleus . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| SKI also facilitates cell cycle progression by targeting the RB pathway by at least two ways : it directly associates with RB and represses its activity when expressed at high levels , and indirectly , it represses Smad mediated induction of p 21 ( Waf 1 ) This results in increased CDK 2 activity , RB phosphorylation , and inactivation . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| In this investigation , we have determined the effect of the DNA demethylating drug decitabine ( DAC ; 5 aza 2 ' deoxycytidine ) on the growth , cell cycle kinetics , RB phosphorylation , and expression of p 16 ( INK4a ) and p 21 ( WAF 1 ) in EBV human myeloma and EBV+ lymphoblastic cell lines possessing silenced , methylated p 16 ( INK4a ) genes to : ( 1 ) . evaluate its potential as a therapeutic agent and ( 2 ) . investigate its mechanism of action . ^^^ Demethylation of the p 16 ( INK4a ) gene and expression of the p 16 ( INK4a ) protein were observed using higher doses ( 10 ( 6 ) 10 ( 7 ) M ) of drug while growth inhibition at lower doses ( IC ( 50 ) =2 10 10 ( 8 ) 4 10 10 ( 8 ) M ) was associated with RB dephosphorylation and increased expression of p 21 ( WAF 1 ) , but not with induction of p 16 ( INK4a ) , or apoptosis . ^^^ Kinetic experiments demonstrated that RB dephosphorylation and the increase of p 21 ( WAF 1 ) preceded the induction of p 16 ( INK4a ) . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| When nuclear export of the actin was induced by 12 O tetradecanoylphorbol 13 acetate , DNA synthesis of the senescent cells increased significantly , accompanied with changes of morphologic and biochemical profiles , such as increased RB protein phosphorylation and decreased expressions of p 21 ( WAF 1 ) , cytoplasmic p extracellular signal regulated kinase 1 / 2 , and caveolins 1 and 2 . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| To assess the cellular mechanisms underlying the mesangial cell proliferation and glomerulosclerosis in progressive human IgA nephropathy ( IgAN ) , we examined the expression of E2F1 , Rb , c Myc , proliferating cell nuclear antigen ( PCNA ) , cyclins ( D 1 , E and A ) , cyclin dependent kinase 2 ( CDK 2 ) and CDK inhibitors ( p 21 ( waf 1 ) , p 27 ( kip 1 ) , 57 ( kip 2 ) and p 16 ( ink4a ) ) by immunohistochemistry in renal biopsy specimens . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| These effects are accompanied by a decreased induction of the proliferation inhibitor , p 21 ( Waf 1 ) , and increased induction of cyclin D 1 and extent of Rb , ERK1 / 2 , and JNK phosphorylation in L6 / RAGE delta cyto myoblasts , the opposite occurring in L6 / RAGE myoblasts . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| The inhibitory effect of S100B on p 38 MAPK translates into a defective induction of the muscle specific transcription factor myogenin and the antiproliferative factor p 21 ( WAF 1 ) , while S100B ' s stimulatory effect on ERK1 / 2 results in stimulation of myoblast proliferation via cyclin D 1 induction and Rb phosphorylation and protection against apoptosis via activation of NF kappaB transcriptional activity . ^^^ |
|
| Interacting proteins: P38936 and P06400 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Apoptotic proteins in tumors that showed positive staining were the highest for Bax ( 99 % ) , Fas ( 92 % ) , FasL ( 87 % ) , Rb ( 87 % ) , p 21 ( WAF 1 ) ( 73 % ) , and p 53 ( 70 % ) , and the lowest for c myc ( 58 % ) and Bcl 2 ( 58 % ) . ^^^ |
|