Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.80116431
We present evidence suggesting that the complex formation between Rb and Tag interferes with the viral DNA replication . 0.80116431^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.56023606
Since several potential phosphorylation sites reside within this region , isoforms of RB protein from cellular lysates with various degrees of phosphorylation were compared with respect to their DNA binding affinity . 0.56023606^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.97735665
Binding depended upon the minimal regions of Rb necessary for its growth suppressive activity , as well as upon the D type cyclin sequence motif shared with Rb binding DNA tumor virus oncoproteins . 0.97735665^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.91089606
Here we show that Rb itself associates with human Dnmt 1 ( hDnmt 1 ) independently of its own phosphorylation status . 0.91089606^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The direct addition of ginsenosides Rb 1 , Rb 2 and Rc mixture ( GNS ) enhanced DNA synthesis . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In this mutant an intact rgl restriction system recognizes unglucosylated hydroxymethylcytosine residues in phage DNA , while the absence of a functional rB restriction function prevents degradation of unmodified DNA containing cytosine . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The CD spectra are in this case characterized by an increase in the positive Cotton effect which is dG + dC dependent up to an rb value around 0 . 10 ( where rb = number of platinum atoms bound per nucleotide ) , followed by a decrease until DNA saturation with platinum is reached . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In the hybrid of Ch . th . thummi 10 Ch . th . piger only those interstitial thummi bands which are known to have a greater DNA content than their homologous piger bands are C banding positive and blue stained by the RB method whereas the homologous piger bands are C banding negative and red stained by RB banding . ^^^ Ch . thummi and piger bands with an equal amount of DNA both show no C banding and stain red by RB banding . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Postnatal growth patterns were followed by changes in weight , DNA , protein and RNA in the cerebral hemispheres ( cortex ) and rest of brain ( RB ) at birth , 21 , 63 and 160 days of age . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Feulgen DNA staining of tumor sections after therapy revealed that the average DNA contents in Rb cells decreased significantly ( P < 0 . 01 ) from 16 . 2 + / 2 . 6 in grade 3 4 to 5 . 3 + / 1 . 2 in grade 1 2 compared to that of the untreated control 18 . 8 + / 3 . 2 . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Simultaneously , we have used DNA PCR to investigate RB promoter deletion in 23 adenocarcinomas and one small cell carcinoma of the prostate . ^^^ We also failed to demonstrate any indication of RB promoter deletion at the DNA level in adenocarcinomas . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The products of the two major suppressor genes p 53 and Rb interact with the oncogene products of the DNA tumour viruses . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Due to the central roles of RB and p 107 in coupling external growth signals with the progression of the cell cycle clock , we have hypothesized that these two proteins might be candidates for mediating the effects of insulin on DNA . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
However , the RB gene per se was normal at the DNA level . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Time course analysis showed that in polyoma infected cells induced to re enter the S phase of the cell cycle the appearance of the phosphorylated forms of Rb coincided in time with the accumulation of large T antigen and preceded DNA synthesis . ^^^ Ongoing DNA synthesis was not required for the cells to phosphorylate Rb , indicating that this post translational modification takes place during the activation of the cellular DNA synthesizing apparatus . ^^^ Our data add more evidence to the proposal that interactions between viral early proteins encoded by DNA tumor viruses and the product of RB may play a pivotal role in the mitogenic effect induced by viral infection . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Identification of a 60 kilodalton Rb binding protein , RBP 60 , that allows the Rb E2F complex to bind DNA . ^^^ We present evidence that the DNA binding of the Rb E2F complex involves another cellular factor . ^^^ Addition of Rb to purified preparations of E2F does not generate an Rb E2F complex that can bind DNA , and in fact , we see an inhibition of the DNA binding ability of E2F . ^^^ On the other hand , addition of Rb to cruder preparations of E2F results in the formation of an Rb E2F complex ( E2Fr ) that can bind DNA and produces a distinct complex in gel retardation assays . ^^^ We have identified and purified a 60 kDa protein that allows the Rb E2F complex to bind DNA , and we show that this 60 kDa protein exerts its effect by directly interacting with Rb . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The disruption of this E2F Rb interaction , as well as a complex involving E2F in association with the cell cycle regulated cyclin A cdk 2 kinase complex , may be a common mechanism of action for the oncoproteins encoded by the DNA tumor viruses . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Because this band contains the retinoblastoma ( RB ) gene , we examined the tumor for aberrations in the RB gene using DNA sequence polymorphism analysis and pulsed field gel electrophoresis ( PFGE ) , but did not detect alterations in the RB gene . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
To examine the consequence of adding one RB allele containing its normal regulatory elements back into representative examples of each of these cancer types , as well as to compare the results to those previously reported using various RB complementary DNA constructs , a neomycin resistant marked 13 chromosome was transferred by microcell fusion . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
RB tumor suppressor gene expression responds to DNA synthesis inhibitors . ^^^ Expression of the RB tumor suppressor gene , whose function is putatively in controlling cell growth , may be regulated by S phase specific inhibitors of DNA synthesis that are commonly used in cell synchronization and cancer chemotherapy . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
DNA samples from 92 unrelated patients with bilateral retinoblastoma were analyzed by Southern blot hybridization with cDNA and genomic clones of the retinoblastoma ( RB 1 ) gene . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The Rb / E2F complex formed in the presence of RBP 60 is able to bind DNA and migrates with a distinct mobility in gel retardation assays . ^^^ This second type of Rb / E2F complex does not form a band shift complex in gel retardation assays and its formation results in an apparent inhibition or loss of the DNA binding activity of E2F . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The effects of covalently bound BPDE residues on the electrophoretic mobilities of the RF 1 DNA form in agarose gels were investigated in detail in the range of binding ratios rb approximately 0 . 0 0 . 06 ( covalently bound BPDE residues / nucleotide ) . ^^^ In this range of rb values , there is a striking difference in the mobilities of ( + ) BPDE and ( ) BPDE adducted phi X 174 DNA in agarose slab gels , the covalently bound ( + ) BPDE residues causing a significantly greater retardation than ( ) BPDE residues . ^^^ Increasing the level of covalent adducts beyond rb approximately 0 . 06 in the case of the ( + ) BPDE enantiomer , leads to further unwinding and a minimum in the mobilities ( corresponding to comigration of the nicked form and the covalently closed relaxed modified form ) at rb 0 . 10 + / 0 . 01 ; at still higher rb values , rewinding of the modified DNA in the opposite sense is observed . ^^^ It is concluded that agarose slab gel techniques are not suitable for determining unwinding angles for ( ) BPDE modified phi X 174 DNA because the alterations in the tertiary structures for rb < 0 . 06 are too small to cause sufficiently large changes in the electrophoretic mobilities . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The ability of the well known morphogen , retinoic acid ( RA ) , as well as 1 , 25 dihydroxy vitamin D 3 ( VD ) , whose receptor complex binds a DNA consensus sequence related to that of the retinoic acid receptor , to regulate expression of the retinoblastoma ( RB ) tumor suppressor gene in a context of induced cell differentiation was characterized . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
This G 1 cell cycle arrest was associated with an accumulation of the unphosphorylated form of the retinoblastoma susceptibility gene product ( Rb ) in good correlation with inhibition of DNA synthesis . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
DNA rearrangement was reported to be present in 1 . 5 12 . 1 % of cases with primary leukemias and the absence of the RB gene product was also observed in 6 . 3 23 . 2 % . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We analyzed 14 native osteosarcoma tissue samples for alterations of the tumor suppressor genes RB 1 and p 53 on the DNA level , and as far as possible , the RNA level . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
It contains p 107 , a nuclear `` pocket ' ' protein with similarities in structure and protein binding properties to RB , and cyclin A , a cyclin believed to play a role in facilitating DNA replication . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
These two transcription factors can recognize the same DNA motif in the adenovirus E2A promoter and can bind to it by themselves or in association with RB . ^^^ Screening these sequences against a DNA database identified their presence in non coding regions of many oncogenes , growth factor genes and in the RB gene itself . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The immortalizing proteins from three distinct DNA tumor viruses ( SV 40 large T antigen , adenovirus E1a , and human papilloma virus Type 16 E 7 ) have been shown to interact with RB protein through two noncontiguous regions comprised of amino acids 393 572 ( domain A ) and 646 772 ( domain B ) . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Intracistronic complementation reveals a new function of SV 40 T antigen that co operates with Rb and p 53 binding to stimulate DNA synthesis in quiescent cells . ^^^ The ability of the oncogene products of DNA tumor viruses to induce DNA synthesis in quiescent cells is thought to depend on their capacity to bind to cellular proteins such as the retinoblastoma suppressor protein Rb and the tumor suppressor p 53 , thereby abolishing the growth arresting properties of these proteins . ^^^ However , detailed analysis , including intracistronic complementation , suggests that at least three functions , Rb binding , a novel second activity localized to the DNA binding domain and a function residing in the carboxy terminus , probably p 53 binding , cooperate to generate the full growth induction potential of T antigen . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In Rb CDM , insulin plus LDL stimulated cell growth by increasing DNA synthesis without changing the synthesis of bulk protein , implying that they induced synthesis of growth related proteins . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
By using three independent sets of amplification reactions , which cover 26 % of the RB 1 gene coding region , we identified RB 1 gene deletions in the DNA of peripheral blood cells in 3 out of 24 ( 12 . 5 % ) unrelated patients with hereditary retinoblastoma . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In the present study , loss of heterozygosity ( LOH ) in RB was found in 26 % of 90 informative primary breast tumors and was correlated to DNA nondiploidy , a high S phase fraction , and LOH at chromosome 17p13 . 3 . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
PURPOSE : To determine genetic alterations at chromosomes 13 and 17 in colorectal tumors , we have studied several loci on these chromosomes , with special focus on the RB 1 and TP 53 genes at both the level of DNA sequence and the level of gene expression . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
These cells showed no RB DNA rearrangement . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Southern blot analysis of DNA from peripheral blood lymphocyte revealed that there was no difference in the expression of Rb gene between the patient and healthy adult . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In human vascular endothelial cells , both growth factor induced DNA synthesis and retinoblastoma gene product ( RB ) phosphorylation are absolutely dependent on extracellular Ca2+ , and are potently inhibited by an active calmodulin antagonist , W 7 , but not an inactive analogue , W 12 . ^^^ A reduction in the extracellular Ca2+ or an addition of W 7 as late as 8 h after growth factor stimulation still inhibits both RB phosphorylation and DNA synthesis to the full extent . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The E 7 ORF encoded an amino acid sequence that is conserved in some DNA tumor viruses and is involved in binding to Rb protein and in cellular transformation . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In contrast , mutations were identified in the p 53 and RB genes expressed in the C 33A and HT 3 cervical cancer cell lines , which lack HPV DNA sequences . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Injection of RB into cells arrested at G1 / S or late in G 1 has no effect on BrdU incorporation , suggesting that RB does not inhibit DNA synthesis in S phase . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
This conclusion was supported by analysis of mRNA and genomic DNA , which revealed that a transcriptionally active RB allele had a deletion of exons 21 27 . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The transforming proteins of several DNA tumour viruses , including adenovirus E1a and simian virus 40 large T antigen , complex with the retinoblastoma ( Rb ) tumour suppressor gene product . ^^^ These results demonstrate that the Rb protein forms a complex with a DNA bound transcription factor , and suggests that the Rb protein might act by regulating transcription . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The RB 1 complementary DNA was under control of the inducible murine metallothionein promoter in MDA 468 S 4 and the thymidine kinase promoter in the retinoblastoma lines . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The Rb protein forms specific complexes with the oncogenes of three different groups of DNA tumour viruses . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Evidence derived from use of expression plasmids for certain DNA viral oncoproteins has suggested that the retinoblastoma gene ( RB ) may be involved in this process . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Detailed analyses of DNA , mRNA , and protein showed that ( 1 ) 3 lines including both Japanese lines lost the expression of the RB protein due to either the absence or the alteration of mRNA caused by DNA rearrangement , ( 2 ) abnormality of p 53 gene was detected in all cell lines : 4 lines lost p 53 expression due to either gene loss or the absence of mRNA , and one line expressed an abnormal form of the protein without detectable DNA and mRNA alterations and ( 3 ) no significant alteration of p 107 or p 300 was detected in all cell lines . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Complexed DRTF 1 , referred to as DRTF1a , has similar DNA binding specificity and DNA binding polypeptides to DRTF1b , which lacks Rb . ^^^ These data show that DRTF 1 is a group of transcription factors that share common DNA binding polypeptides and which complex with other non DNA binding proteins , such as the Rb protein and cyclin A , in a developmentally regulated and tissue dependent fashion . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
When normal human peripheral blood lymphocytes were mitogenically stimulated with pokeweed mitogen , bivariate flow cytometric measurements of cellular DNA and RB protein content showed an early decrease in the amount of RB protein per cell , anteceding onset of S phase . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
After reviewing the use of chemicals , physical agents , oncogenes and DNA tumor viruses as immortalizing agents , we consider the importance of negative regulators of cell growth ( RB and p 53 ) , their inactivation , as well as genomic instability in the pathogenesis of cancer . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
When stimulated with serum , phosphorylation of the RB protein occurred between 10 20 h after stimulation , which corresponded with the induction of DNA synthesis . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
No gross alterations of the RB gene were found at DNA level in any of the examined specimens . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The retinoblastoma ( Rb ) gene , thought by some to be associated with tumor formation of retinoblastoma as a recessive human oncogene , was investigated in 57 cases using DNA and RNA from primary osteosarcomas and other bone and soft tissue tumors . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The RB gene product , pp110RB , is a nuclear phosphoprotein with DNA binding activity . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
After stimulation with phorbol , 12 , 13 , dibutyrate and the calcium ionophore , ionomycin , which triggers a rapid entry of cells into G 1 phase , we demonstrated Rb phosphorylation 24 h later , well before the onset of DNA synthesis . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Alterations within the retinoblastoma ( Rb ) gene , as detected by the VNTR probe p68RS2 . 0 , and flow cytometric DNA pattern have been analysed in 255 colorectal carcinomas . ^^^ Sixty three per cent of the carcinomas were DNA aneuploid , and a significant association was demonstrated between amplification within the Rb gene and DNA aneuploidy ( P less than 0 . 01 ) . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In this study , we investigated the involvement of the RB gene in bladder and renal cell carcinomas by determining the loss of heterozygosity ( LOH ) at the RB locus and by DNA , RNA , and protein analysis of the RB gene . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We investigated DNA from 61 primary human esophageal tumors for loss of heterozygosity at the Rb locus using a polymerase chain reaction based restriction fragment length polymorphism assay . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Polyclonal antibodies that bind selectively to adducts formed with DNA by chemotherapeutically inactive trans diamminedichloroplatinum ( 2 ) [ trans [ Pt ( NH 3 ) 2Cl2 ] ) were produced by immunization with calf thymus double helical DNA modified by trans [ Pt ( NH 3 ) 2Cl2 ] at a ratio of bound platinum / nucleotide ( rb ) of 0 . 1 . ^^^ The antibodies were competitively inhibited in an ELISA assay by 25 pM trans [ Pt ( NH 3 ) 2Cl2 ] bound to double helical DNA and 2 . 5 pM trans [ Pt ( NH 3 ) 2Cl2 ] bound to denatured DNA ( rb = 0 . 1 ) . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
On the other hand , nuclear localization and DNA binding activity were preserved in the mutated protein . p 104 was immunoprecipitable with four separate polyclonal antibodies recognizing different epitopes of the RB polypeptide , suggesting the presence of most exons in their correct reading frame . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In 10 / 14 informative constitutional DNA RB tumor DNA pairs , a reduction to homozygosity allowed identification of the RB 1 allele at risk to carry a germline RB 1 mutation . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Chlamydia trachomatis elementary body ( EB ) and reticulate body ( RB ) developmental stages have polymorphic plasmid DNA . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
To examine the association of deletions in the Rb 1 gene with tumor grade , we correlated the DNA alterations in the Rb 1 gene with clinical data for 36 patients . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
PCR SSCP analysis of the genomic DNA also revealed that Lu 65 cells contained the mutated RB allele , but not the normal allele . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We show that the phosphorylation state of the RB protein in cells with 2N DNA content depends on whether the cells are actively cycling . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
How Rb acts to bring about this suppression is not clear but one clue is that the Rb protein forms complexes with the transforming oncoproteins of several DNA tumour viruses , and that two regions of Rb essential for such binding frequently contain mutations in tumour cells . ^^^ The apparent targeting of the RbAP 46 Rb interaction by oncoproteins of DNA tumour viruses strongly suggests that formation of this complex is functionally important . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Inheritance of this mouse mutation in crosses segregating the Rb ( 12 . 14 ) 8Rma translocation chromosome and various DNA markers of Chromosome 12 have localized cpk to a site near D12Nyu2 , approximately 7 cM from the centromere of Chromosome 12 . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Deletion in one allele and a rare neutral DNA alteration in the other allele of the RB 1 gene in a patient with bilateral retinoblastoma . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Wild type RB complementary DNA was introduced into Weri RB 27 by retrovirus mediated gene transfer . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We report measurements of the water content and swelling of wet spun films of Na , K , Rb , and Cs DNA as a function of relative humidity ( rh ) . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In addition , the DNA region essential for pUK 318 replication in Rb . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Recently , transforming proteins from three different classes of DNA tumor viruses have been shown to form complexes with the RB protein . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The DNA tumor viruses have evolved mechanisms to interact with the Rb and p 53 negative regulators of cellular growth in order to enhance their own replication in growing cells . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
A link may be provided by the finding of a direct interaction of certain DNA tumour virus oncoproteins with the RB gene product p 105 RB , together with observations that the tumour viruses are also able to reinitiate DNA synthesis in growth arrested senescent fibroblasts . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
By using viral mutants , we now show that the induction of cellular DNA synthesis in quiescent cells by E1A differs from transformation in that E1A products induce synthesis if they are able to bind to either p 105 Rb or p 300 , and only mutant products that bind to neither are extremely defective . ^^^ These results suggest that p 105 Rb and p 300 ( or cellular proteins with similar E1A binding properties ) provide parallel means by which DNA synthesis can be regulated . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Both RB 1 and p 53 gene products are bound by various transforming early proteins encoded by the DNA tumour viruses SV 40 , adenovirus and human papilloma virus . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The ability of simian virus 40 T antigen to mediate transcriptional activation and viral DNA replication was tested in human osteosarcoma cell lines U 2OS and Saos 2 , which are Rb positive and Rb negative , respectively . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Rabbit aortic smooth muscle cells ( SMC ) and Rb 1 cells , a continuous line of the same origin , were transformed by transfection with pSV 3 neo DNA , a plasmid containing the SV 40 early region linked to the neoR resistance gene . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Our studies of 26 SCLC tumor and normal DNA samples indicate that 6 of 6 patients whose normal cell DNA was heterozygous for an RB restriction fragment length polymorphism have lost one of the two alleles in their tumor DNA . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Gross DNA alterations were found in four tumors , of which only one expressed Rb mRNA . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The retinoblastoma susceptibility gene ( RB ) encodes a phosphoprotein of 110 kd ( pp110RB ) that forms specific complexes with SV 40 T antigen and the transforming proteins of several other DNA tumor viruses . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In addition , although the actions of putative tumor suppressor genes are less well understood , the first isolated tumor suppressor gene Rb , interacts with the products of DNA viruses which , in turn , are involved in regulation of transcription as was described in Section II . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In Rb ( + ) CDM , cell growth and DNA synthesis were greatly suppressed , although cell viability was not significantly altered for 72 h . ^^^ After cessation of cell growth in Rb ( + ) CDM for 24 h , addition of insulin and / or LDL markedly restored cell growth and DNA synthesis . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Ability of the HPV 16 E7 protein to bind RB and induce DNA synthesis is not sufficient for efficient transforming activity in NIH3T3 cells . ^^^ In conjunction with previously described mutants in the region of E 7 with similarity to E1a CD 2 and SV 40 LT , we have investigated three known activities of the E 7 protein ; transformation , association with the cellular RB protein and induction of cellular DNA synthesis . ^^^ The results show that RB binding correlates with the ability of E 7 to induce cellular DNA synthesis and mediate cell transformation . ^^^ In addition an unidentified function of E 7 , which is necessary for transformation of NIH3T3 cells , but does not affect RB binding or the ability to induce cellular DNA synthesis , has also been demonstrated . ^^^ One of these , in the region of E 7 which shows similarity to E1a CD 2 and LT , is required for RB binding and DNA synthesis . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
To determine whether the RB gene might be involved in the pathogenesis of human ovarian and endometrial cancer , DNA from 24 human ovarian tumors , 3 normal ovaries , 3 endometrial carcinomas , and 1 endometrial hyperplasia was examined with an RB complementary DNA probe . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
To define this mutation , the RB complementary DNA and genomic DNA were sequenced , revealing a point mutation in exon 21 that changed a G to a T . ^^^ The mutant RB complementary DNA was used as a template for in vitro transcription and translation to synthesize the mutated protein . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Upon insertion of the cloned human RB complementary DNA sequence into the viral genome downstream of the promoter of the polyhedrin gene , full length RB protein with an apparent molecular weight of 110 , 000 was expressed in the insect cells . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The hydrolysis of denatured platinum DNA complexes , rb = 0 . 075 platinum per nucleotide , obeyed Michaelis Menten kinetics . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Thus , Rb phosphorylation appears linked to the ability of a cell to synthesize DNA . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Although the chromosomal imbalance makes these MESC lines unsuitable for genetic manipulation in vitro and hence for subsequent production of transgenic animals , the spontaneous occurrence and stable retention of the homologous Rb ( 11 . 11 ) as the only metacentric chromosome in an otherwise all acrocentric karyotype , provides potentially useful cell lines for gene assignment and recombinant DNA studies . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Southern blot analysis of genomic DNA extracted from HSB 2 cells showed a large homozygous deletion of the RB gene , stretching from exon 18 beyond exon 27 . ^^^ The genomic deletion of greater than 85 kb DNA at the RB locus ( 13q14 ) was not detectable in the karyotype of the HSB 2 cells . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
After an 8 h incubation of L 1210 cells with 0 . 1 microM elliptinium ( the dose corresponding to the 50 % inhibitory dose ) , we obtained 2 . 4 10 10 ( 6 ) and 3 . 4 10 10 ( 6 ) as the rb for RNA and DNA , respectively . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The RB protein is a nuclear phosphoprotein capable of binding to DNA and forming a complex with oncoproteins of several DNA tumor viruses . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
It has been shown that RB protein is also a key target for transformation by the oncogenes of several small DNA tumor viruses . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
This is analogous ( in cell culture ) to the observations made in tumors ( in vivo ) discussed above . ( 3 ) Both the tumor suppressor gene , the retinoblastoma sensitivity gene or Rb and p 53 are found in oligomeric protein complexes with the oncogene products of the DNA tumor viruses . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
By means of a rapid method for the detection of mutations in Rb mRNA , this defective Rb protein was observed to result from a single point mutation within a splice acceptor sequence in J 82 genomic DNA . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The product of the retinoblastoma ( RB ) susceptibility locus suppresses neoplastic growth and exists as a family of differentially phosphorylated , DNA binding proteins . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Recently , the RB 1 gene product , p 105 RB , has been shown to form stable protein / protein complexes with the oncoproteins of two DNA tumor viruses , the adenovirus E1A proteins and the simian virus 40 ( SV 40 ) large T antigen . ^^^ These results suggest that these three DNA viruses may utilize similar mechanisms in transformation and implicate RB binding as a possible step in human papilloma virus associated carcinogenesis . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Structural abnormalities to RB 1 were detected in DNA from 15 / 77 ( 19 % ) of primary breast carcinomas examined . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Since gene activity can be inhibited by DNA methylation , and inactivation of tumor suppressor genes is a fundamental process in oncogenesis , we investigated the methylation status of the retinoblastoma suppressor gene ( RB gene ) on chromosome 13 , in blood and tumor cells from 21 retinoblastoma patients . ^^^ Using methylation sensitive restriction enzymes and a cloned DNA probe for the unmethylated CpG island at the 5 ' end of RB gene , we obtained evidence of hypermethylation of this gene in a sporadic unilateral retinoblastoma tumor . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In addition , a binding between the RB product and various viral onco proteins ( E1A , large T , E 7 ) has been demonstrated , thus suggesting a mechanism of RB inactivation by which some DNA viruses can transform the host cell . ( ABSTRACT TRUNCATED AT 400 WORDS ) . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We were unable to detect expression of the mutant allele in lymphoblasts of three bilaterally affected patients , although the mutation was present in the genomic DNA and transcripts containing the mutations were obvious in the RB tumors in the absence of a normal RB 1 allele . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Using DNA from clinical isolates of all six serotypes ( a f ) and a genetically defined capsule deficient recipient strain Rb : 02 , we constructed a series of capsular transformants otherwise identical with respect to outer membrane protein and LPS subtype , biotype , and electrotype . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Interestingly , the product of the Rb gene has recently been shown to associate with the transforming proteins of DNA tumor viruses . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Another possibility related with the DNA binding properties of Rb and L 1 family proteins is also discussed . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
DNA rearrangement and abnormal RB mRNA were detected in two of these three cell lines , whereas RB protein was absent from one synovial sarcoma cell line in which normal sized RB mRNA was clearly present . ^^^ Therefore , direct immunoprecipitation of RB protein can reveal RB gene mutations that are undetectable by DNA and mRNA analysis . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Likewise , titration of NaDNA solutions with MgCl 2 shows that condensation of Mg2+ on the DNA double helix reduces Rb . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The RB transcript is encoded in 27 exons dispersed over about 200 kilobases ( kb ) of genomic DNA . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
An additional tumor lacked the RB transcript , but the gene appeared intact at the DNA level . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Using a human IRBP complementary DNA probe , levels of IRBP RNA transcripts in 19 RB cell lines were comparable to adult retina . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
To determine whether the retinoblastoma gene ( Rb ) localized at 13q14 , might be the target of recessive mutations in lung cancer , eight primary SCLC tumors and 50 cell lines representing all major histologic types of lung cancer were examined with the Rb complementary DNA probe . ^^^ Rb messenger RNA expression was absent in 60 % of the SCLC lines and 75 % of pulmonary carcinoid lines , including all samples with DNA abnormalities . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Nine lung small cell carcinoma ( SCC ) cell lines and 9 lung non SCC cell lines were examined for structural changes of the retinoblastoma ( RB ) gene as well as its expression using a complementary DNA probe . ^^^ Although homozygosity or hemizygosity of the RB gene was suggested in 8 of 9 SCCs and one of 2 large cell carcinomas ( LCCs ) by Southern blot analysis using an RB cDNA probe and polymorphic DNA markers for chromosome 13 , no obvious structural changes of the RB gene were detected in these 18 cell lines . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Sixteen of 40 retinoblastomas examined with a complementary DNA probe shown to be the Rb gene had identifiable structural changes of the Rb gene including in some cases homozygous internal deletions with corresponding truncated transcripts . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In order to further characterize the RB gene and its structural alterations , we examined genomic clones of the RB gene isolated from both a normal human genomic library and a library made from DNA of the retinoblastoma cell line Y 79 . ^^^ Second , two overlapping genomic clones that demonstrated a DNA deletion of exons 2 through 6 from one RB allele were isolated from the Y 79 library . ^^^ To confirm and extend this result , a unique sequence probe from intron 1 was used to detect similar and possibly identical heterozygous deletions in genomic DNA from three retinoblastoma cell lines , thereby explaining the origins of their shortened RB mRNA transcripts . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
To extend the characterization of this gene , we used 4 . 7R probes to search for genomic rearrangements of DNA and to study the expression of the 4 . 7R gene in RB tumors , osteosarcoma ( OS ) tumors arising in RB patients , and other normal and malignant tissues . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
To elucidate the mechanism by which this abnormal chromosome is formed , 24 RB tumors and three cell lines were studied by means of DNA polymorphisms specific for the short arm and the long arm of chromosome 6 . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
A candidate DNA sequence with many of the properties predicted for the retinoblastoma susceptibility ( RB 1 ) locus has been cloned ( S . ^^^ Here we used field inversion gel electrophoresis ( FIGE ) to construct a restriction map of approximately 1 , 000 kb of DNA surrounding the RB 1 locus and to detect the translocation breakpoints in three retinoblastoma patients . ^^^ Both probes identified an additional hybridizing fragment in the DNA from each patient , permitting the breakpoints in all three to be mapped within the cloned RB 1 gene . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
This mutant could not support viral DNA replication , but could immortalize secondary cultured rat brain ( RB ) cells . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The RB gene product was identified as a nuclear phosphoprotein of 110 kD associated with DNA binding activity , suggesting that the RB protein may regulate other genes . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We investigated the ability of nine fibroblast cell strains from patients with the hereditary form of retinoblastoma ( RB ) to handle various types of DNA damaging agents and compared the results with those obtained in nine normal strains . ^^^ Three RB cell strains derived from individuals who had either developed second cancers or who had a family history of additional sarcomas consistently exhibited increases in sensitivity to all three DNA damaging agents studied compared with other hereditary RB cell strains as well as normal strains . ^^^ Most hereditary RB strains exhibit sensitivity well within the normal range ; however , strains from RB patients predisposed to second cancers exhibit increases in sensitivity to DNA damaging agents . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
DNA and RNA were extracted from primary human osteosarcomas and soft tissue sarcomas obtained from patients without retinoblastoma and were analyzed by hybridization with a cDNA probe for RB mRNA ; absence or alterations of the RB gene are associated with development of retinoblastoma . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Five of 11 RB tumors , which exhibit normal 4 . 7R DNA and normal sized RNA transcripts , showed abnormal ribonuclease cleavage patterns . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
No obvious defect in RB 1 was detected by DNA blot analysis in primary tumors or cell lines from Wilms ' tumor , cervical carcinoma , or hepatoma . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
With the large Centre d ' Etude Polymorphisme Humain cohort , a refined map of DNA markers from this region was constructed , with the following locus order : D13S1 D13S21 D13S22 D13S10 ESD RB WND D 13S26 D13S12 D13S2 . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Here , we show that Ta DNA transcripts are present in Fanconi ' s anemia ( FA ) and retinoblastoma ( Rb ) fibroblasts , at a level hardly lower than in tumor cells . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Sequence analysis of RB complementary DNA clones demonstrated a long open reading frame encoding a hypothetical protein with features suggestive of a DNA binding function . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Moreover the total A T ( DAPI ) and DNA ( PI ) content was quantitatively evaluated , by flow cytometry , in G0 / G1 nuclei belonging to four different Rb mouse populations , karyotypically characterized by the presence of up to nine Rb chromosomes . ^^^ We also discuss the possibility that the high rate of Rb exchange in the house mouse could be favoured by the simultaneous effects of undetectable losses of chromosomal material , high repetitiveness of the DNA involved , the presence of the same major type of satellite DNA over each chromosome and the all acrocentric constitution of the karyotype . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The in vitro experiments on CaOv cells demonstrated that ribamidyl ( ribavirine ) ( Rb ) enhances the intracellular pool of pyrimidine precursors of DNA . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
A comparison of the magnitude of phi below rb values of 0 . 005 and those at high levels of binding reveals that the extent of torsional strain in the supercoiled DNA influences the magnitude of the unwinding of the DNA by these complexes . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In double stranded cis DDP DNA with low Pt : nucleotide ratios ( rb ' s ) , this accessibility is enhanced by unwinding of the cis DDP DNA , e . g . by heat denaturation . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Host specificity of DNA in Haemophilus influenzae : the restriction and modification systems in strains Rb and Rf . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In the spectrum of RG stained nuclei , pH congruent to 7 , three absorption bands are distinguishable : They are A 1 ( 15400 cm 1 , 649 nm ) , A 2 ( 16800 cm 1 , 595 nm ) the absorption bands of DNA bound monomers and dimers of azure B and RB ( 18100 cm 1 , 552 nm ) the distinct intense Romanowsky band . ^^^ Our extensive experimental material shows clearly that RB is produced by a complex of DNA , higher polymers of azure B ( degree of association p greater than 2 ) and eosin Y . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Spectrophotometric titrations suggested at least two types of chromophore binding sites on DNA ; one type of site was saturated at rb = 0 . 125 chromophore molecule / nucleotide , but binding to additional sites continued to at least rb = 0 . 3 . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In contrast , the lifespan of one Rb cell line ( Rb 1 ) was prolonged ; irradiated cells with an increased growth potential showed a higher number of cells at confluency and more cells were entering DNA synthesis phase than in non irradiated cells . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Ten analogues of RB 3 had a similar inhibitory effect on DNA synthesis in N18TG 2 cells . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The macronuclear RB is composed of two zones : a `` forward zone ' ' showing a special chromatin organization consisting of regular 40 to 50 nm diameter fibers with an indication of nucleosomal substructure ; and a `` rear zone ' ' , the site of DNA replication , consisting of a mesh of 10 nm chromatin fibers . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The effects of ginsenosides Rg 1 and Rb 1 of Panax ginseng on mitosis in the onion root tip cells as well as on the rate of DNA synthesis in onion seedlings were studied . ^^^ Rb 1 was found to shift the peak hour of DNA synthesis to a later period of the experiment . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
DNA diagnostic techniques are being introduced rapidly into the clinical laboratory setting . 1 ) In lung cancer , several oncogenes and tumor suppressor genes , such as ras , myc , p 53 , RB , allelic loss of chromosomes , play very important roles . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Specimens from 42 patients were analyzed using both DNA probes specific to the centromeric region of chromosome 12 and the retinoblastoma ( RB ) gene . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We did not detect any mRNA for beta actin , retinoblastoma ( RB ) , CD 4 , or kappa light chain genes in the sperm complementary DNA samples , verifying that the class 1 mRNA detected was not due to somatic cellular contamination of the purified sperm samples . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Although measurements of mucosal colonic weight and RNA content were significantly different between groups fed RB and WB ( p < 0 . 05 ) , DNA content and the ratio RNA / DNA did not significantly differ between these groups . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Hypophosphorylation of retinoblastoma protein ( RB ) accompanies the DNA damage induced , p 53 independent G 1 arrest and apoptosis in two p 53 null human leukemic cell lines , HL 60 and U 937 ( Q . ^^^ When an HL 60 cell line resistant to cytosine arabinoside was exposed to this DNA damaging agent , neither RB hypophosphorylation nor apoptosis were observed . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The pocket was originally identified as the region of Rb required for binding to oncoproteins from DNA tumour viruses , which disrupt the binding of Rb to the E2F family of cell cycle transcription factors ( referred to collectively here as E2F ) . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
These results show that the segregation analysis of RB 1 gene loci with specific RFLPs , used as the DNA markers , could be helpful in the genetic diagnosis and counselling in families with hereditary retinoblastoma . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Failure to prevent entry into S phase in Rb / MEFs exposed to DNA damaging or nutrient limiting conditions caused apoptosis and correlated with p 53 induction . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
METHODS : Molecular analysis was performed on 103 DNA samples of 15 Rb families . ^^^ FINDINGS : DNA analysis was informative in 88 % of relatives at risk of developing Rb . ^^^ CONCLUSION : In the majority of familial Rb , the DNA analysis allows the identification of children carrying a RB 1 mutation and who will need a close ophthalmologic follow up under general anesthesia . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Comparison of the two models reveals that tau perpendicular infinity S 2 tau rb , where tau rb represents the rigid body diffusion of the DNA helix . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In addition , overexpression of a complementary DNA ( cDNA ) for the Rb messenger RNA was sufficient to slow the proliferation of HeLa cells , and the level of Rb cDNA expression was correlated with the degree to which the rate of growth of the tumor was slowed . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
A T PMCs were exposed to p 53 and / or Rb sense , scrambled DNA and antisense oligodeoxynucleotides . p 53 and / or Rb antisenses ( but not their senses or scrambled DNA ) treatment of A T PMCs resulted in a significantly increase in the number of granulocyte / macrophage colony forming units ( CFU GM ) in the presence of interleukin 3 ( IL 3 ) and / or granulocyte / macrophage colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In the present study , we isolated two overlapping genomic DNA clones ( termed c13S25 ) containing the D13S25 DNA segment and used them as probes to analyze 85 B CLL cases by fluorescence in situ hybridization ; of the 55 cases with two RB 1 copies , 13 exhibited hemizygous ( n = 7 ) or homozygous ( n = 6 ) deletion of D13S25 . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Abnormal expression of the Rb protein ( in fewer than 90 % of cells ) was independent of the T category while there was a significant relationship between DNA ploidy ( P = 0 . 0138 ) , S phase fraction ( P = 0 . 0042 ) and expression of the Rb protein . ^^^ Abnormal expression of the Rb protein had no prognostic value while T category ( P = 0 . 0017 ) , Gleason score ( P = 0 . 0097 ) , DNA ploidy ( P = 0 . 0034 ) , S phase fraction ( P = 0 . 0179 ) and mitotic index ( P = 0 . 0001 ) were significant prognostic factors . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We find that c Abl requires p 53 but not Rb to suppress growth . c Abl binds p 53 in vitro and enhances p 53 dependent transcription from a promoter containing p 53 DNA binding sites . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Sphingosine treatment of Molt 4 cells , but not WERI Rb 1 cells , resulted in the loss of the specific E2F bands produced by the interaction of E2F and its specific DNA sequence element on gel shift assays . ^^^ These results demonstrate that activation of Rb by sphingosine leads to sequestration of E2F by the active ( hypophosphorylated ) form of Rb with the resultant loss of its DNA binding and genetranscribing abilities . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Regardless of whether mitotic or meiotic preparations were used , in situ hybridisation failed to reveal pericentromeric telomeric sequences on any of the Rb chromosomes , while all metacentrics retained detectable , although reduced ( average 50 kb ) , amounts of minor satellite DNA in the vicinity of their centromeres . ^^^ Pulsed field gel electrophoresis and Southern analysis of DNA from Rb mice showed that the size of the telomeric arrays is similar to that of mice with all telocentric chromosomes and that the minor satellite sequences were hybridising to larger fragments incorporating major satellite DNA . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The mechanism by which these animal viruses create a cellular environment permissive for viral DNA replication involves the binding of a virally encoded oncoprotein , through its LXCXE motif , to the retinoblastoma protein ( Rb ) . ^^^ The presence of an intact Rb binding motif is required for efficient WDV DNA replication in cultured wheat cells , strongly suggesting that one of the functions of WDV Rep may be the linking between viral and cellular DNA replication cycles . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
High risk E 6 and E 7 bind and functionally inactivate tumor suppressor proteins p 53 and Rb , respectively , and both disrupt the G 1 arrest in response to DNA damage . ^^^ The increased spontaneous mutagenesis , therefore , appeared to be mediated by p 53 inactivation and to be independent of Rb ( which acts downstream of p 53 in the G 1 arrest pathway following DNA damage ) . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
No abnormalities of RB 1 , p 53 and p16INK4 were detected in cell lines containing DNA tumour virus proteins known to bind to the protein products of the RB 1 and p 53 genes . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Previously , we have reported two major alpha 2 adrenergic receptor transcripts in rat brain of 3 . 8 and 3 . 0 kb and the cloning and characterization of the rat brain complementary DNA ( cDNA ) ( RB alpha 2C ) specific for the 3 . 0 kb messenger RNA . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
To examine the effect of a Rb DNA bound complex on transcription , full length Rb was fused to the DNA binding domain of GAL 4 . ^^^ Here , we report that GAL 4 Rb can repress transcription mediated by either Sp 1 , AP 1 , or p 53 , dependent upon the presence of both the GAL 4 DNA binding domain and GAL 4 binding sites . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Mutagenesis of the DNA encoding two of these peptides followed by screening in the two hybrid system allowed the delineation of residues apart from the invariant Leu , Cys and Glu that affect binding to Rb . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
These results reveal that diverse growth signals work through Rb to regulate entry into S phase , and they indicate that absence of Rb protein produces a constitutive DNA replication signal capable of activating a p 53 associated apoptotic response . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Abnormal expression of Rb protein was significantly related to grade ( P < 0 . 00004 ) , type ( P = 0 . 0183 ) , margin formation ( P = 0 . 0116 ) , DNA ploidy ( P < 0 . 0002 ) and nuclear pleomorphism ( P < 0 . 0001 ) . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The ability of RB 90740 to cause DNA strand breaks under hypoxic conditions was confirmed by alkaline sucrose gradient and pulsed field gel electrophoresis techniques . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Mutational analysis showed that the ability of E2F 1 to bind to DNA was necessary and sufficient to block the formation of large cells by RB , whereas the ability to induce S phase entry required a functional transactivation domain as well . ^^^ Furthermore , the ability of the E2F 1 DNA binding domain alone to block one manifestation of RB action is consistent with the notion that RB E2F complexes actively repress transcription upon binding to certain E2F responsive promoters . ^^^ In keeping with this view , we show here that coproduction of an E2F1 mutant capable of binding to DNA , yet unable to transactivate , is sufficient to block RB mediated transcriptional repression . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Changing the tumor suppressor function of Rb by expressing transcription factor E2F1 and viral oncoprotein E1A in cerebellar granular neurons in vitro and in cerebral cortical neurons in vivo results in the induction of DNA synthesis in these neurons . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
However , in cells in which the function of Rb has been compromised , either by naturally arising mutations or through binding to proteins encoded by DNA tumour viruses , Cdk 4 and Cdk 6 are not associated with D cyclins . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
As actively growing cells require the ongoing synthesis of ribosomal RNA , we considered that Rb might interact with the ribosomal DNA transcription apparatus . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We used total genomic DNA ( blood and tumors ) to investigate the power of this PCR Rb Xbal RFLP in the identification of both segregation and loss of heterozygosity of the Rb gene . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Formation of 8 hydroxydeoxyguanosine from 2 ' deoxyguanosine and its decomposition by active oxygen ] . 8 hydroxydeoxyguanosine ( 8 OHdG ) was formed from dG and isolated DNA by photosensitization with rose bengal ( RB ) and methylene blue ( MB ) . 8 OHdG formed from dG was decomposed by the photosensitization with these dyes . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
DNA copy number abnormalities detected in this study include previously identified increases at 1p22 32 ( L myc ) , 2p24 25 ( N myc ) , and 8q24 ( c myc ) and decreases at 17p13 ( p 53 ) , 13q14 ( RB ) , and 3p . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The molecular basis of this insufficiency would be damage to genomic ( but also other cellular ) structures and closing of specific signalling pathways or opening of others ( like those leading to apoptosis ) . 1 describe experimental data that suggest an important role of RAS / NF1 and p53 / p105 Rb proteins in cell cycle control coupled responses to DNA damage . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Several analyses of the retinoblastoma ( RB ) gene in lung cancer at the DNA , mRNA and protein levels have recently been reported . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We demonstrate that MyoD mediates the transactivation of a CAT construct whose expression is driven by the human Rb 1 gene promoter , and that this is not a consequence of direct binding of MyoD to an E box DNA sequence motif present in the Rb 1 promoter sequences . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Tumors with LOH at Rb 1 . 20 were examined for mutations in the remaining Rb allele using single strand conformational polymorphism ( SSCP ) analysis and direct DNA sequencing . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Rb and p 53 proteins were studied by immunoprecipitation and Western blotting experiments in the presence and absence of DNA damaging treatment . ^^^ The phosphorylation status of Rb was altered following DNA damage in p 53 wild type cell lines , but was not altered in p 53 mutant cell lines , nor in cell lines where p 53 function was abrogated by viral gene products . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
A T DNA vector for plant transformation has been constructed in which the cloning site is located 9 bp from the right border ( RB ) end and 27 bp from the left border ( LB ) end . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We have examined mitochondrial DNA ( mtDNA ) sequence variation from the control region and the ND 3 gene region among 56 M . domesticus from Western Europe , including 15 Rb populations and 13 standard karyotype populations , and two individuals of the sister species , Mus musculus . mtDNA exhibited an average sequence divergence of 0 . 84 % within M . domesticus and 3 . 4 % between M . domesticus and M . musculus . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Preincubation of crude nuclear extracts with monoclonal antibodies to RB results in reduction of Sp 1 complexes in a mobility shift assay , while addition of recombinant RB in mobility shift assay mixtures with CCL 64 cell extracts leads to an enhancement of DNA binding activity of SP 1 . ^^^ These results suggest that RB is directly or indirectly involved in Sp 1 DNA binding activity . ^^^ Finally , we have identified Sp 1 1 , a approximately 20 kDa protein ( s ) that inhibits the Sp 1 complexes from binding to DNA and that is also an RB associated protein . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The later addition of AGM 1470 up to 3 h after the growth factor stimulation still exerts full inhibitory effects on both DNA synthesis and RB phosphorylation , suggesting that the major site of action of AGM 1470 is located relatively late in the G 1 phase . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We also found that apoptotic mouse 3T3 fibroblasts express c fos , c myc , c jun , and cdc 2 , as well as the upregulation of RB phosphorylation and BrdU incorporation , just before final DNA fragmentation and death . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Our results demonstrate that large and small DNA viruses have a common interface with the host cell , namely the association with the RB tumour suppressor protein . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Polymerase chain reaction ( PCR ) amplification of polymorphic regions in introns 17 and 20 of the human Rb gene was used to detect heterozygosity in pituitary tumor DNA and matched control DNA samples . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Segregation analysis within the RB gene and on chromosome 13 was useful for DNA diagnosis of susceptibility to RB in relatives of hereditary patients , even if mutations were not identified . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Small DNA viruses are dependent on the interaction of early proteins ( such as large T antigen ) with host p 53 and Rb to bring about the G 1 to S cell cycle transition . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The transformation caused by E2F 1 correlates with constitutive overexpression of the transgene , increased transcription of E2F dependent genes and the enhancement of two E2F DNA binding complexes containing the retinoblastoma susceptibility gene product ( Rb ) and E2F 1 . ^^^ The oncogenic potential of E2F 1 is dependent on functional DNA binding and transactivation domains but does not require the ability to interact directly with Rb . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In addition , we observed integration of human papilloma virus ( HPV ) DNA in the single EPSC cell line that retained wildtype RB protein . ^^^ We did not detect integration of HPV , SV 40 or adenoviral DNA in other tumor samples with wildtype RB status . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Although their normal nontransformed counterparts are sensitive to cell cycle arrest mediated by CeReS 18 , cell lines lacking a functional RB protein , through either genetic mutation or DNA tumor virus oncoprotein interaction , are less sensitive . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
DNA probes for the NRAS , HRAS , KRAS 2 , LCK , RAF 1 , MET , MYCL 1 , MYCN , MYB , ERBB 2 , FOS , CSF1R , and SRC protooncogene loci ; the retinoblastoma gene locus ( RB 1 ) ; the tumor virus integration sites INT 2 , PVT 1 , and MLV 12 ; and the locus of the tumor specific antigen T1A were used to screen mouse genomic DNAs from RF / J , CAST / Ei , MOLF / Ei , Mus musculus musculus , M . m . poschiavinus , and M . spretus . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Utilization of a polymorphic variable number tandem repeat DNA region within the RB 1 gene for early diagnosis of hereditary retinoblastoma . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We investigated DNA from 15 human lung tumors for loss of heterozygosity of the Rb locus using a polymerase chain reaction ( PCR ) based restriction fragment length polymorphism assay . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Parathyroid carcinomas from 9 patients and adenomas from 21 were studied for evidence of tumor specific loss of RB gene DNA ( allelic loss ) by analysis of four DNA polymorphisms and for evidence of altered expression oF RB protein by immunohistochemical staining . ^^^ All of 11 specimens from 5 patients with parathyroid carcinoma and informative DNA patterns and 1 of 19 specimens from 19 patients with parathyroid adenoma and informative DNA patterns lacked an RB allele . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In embryonic fibroblasts , DNA replication started 10 15 h after exit from mitosis and RB was transiently dephosphorylated during the G 1 phase as previously described . ^^^ In ES cells , DNA replication started 2 h after release from the colcemid block but virtually no hypophosphorylated RB was observed after the release . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The JCV T antigen is less efficient than its SV 40 counterpart at transforming Rat 2 cells and at binding Rb and viral DNA . ^^^ A JCV T antigen mutant having an SV 40 like Rb binding domain exhibited increased DNA binding activity while , unexpectedly , displaying decreased Rb binding and wild type transforming behavior . ^^^ A mutant T antigen that was unable to bind Rb exhibited decreased DNA binding and failed to transform Rat 2 cells . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The molecular cloning and initial characterization of the complementary DNA encoding the chicken homologue of the human retinoblastoma susceptibility gene product Rb are described . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The RB reconstituted Saos 2 and 5637 cells showed only slightly lower ratios of cells undergoing DNA synthesis compared to their parental RB tumor cells , and there were no noticeable changes in cell morphology . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
To dissect the pathways by which p 53 and Rb may act , the E 6 and E 7 oncogenes of human papillomavirus ( HPV ) types 6 and 16 were introduced into primary human epithelial cells by retroviral transfer vector , and cells were assayed for growth arrest after DNA damage induced by actinomycin D . ^^^ Cells expressing HPV 16 E6 ( 16E6 ) were severely reduced in p 53 protein levels that did not increase detectably after DNA damage , Rb protein levels did not decrease , and hyperphosphorylated Rb was present . ^^^ After DNA damage in cells expressing 16E7 p 53 levels increased , and Rb protein levels decreased ; however , Rb was predominantly in the hyperphosphorylated state . ^^^ This suggests that the circuitry controlling the growth arrest signal after DNA damage may be interconnected between the p 53 and Rb pathways . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Findings in primary tumors were correlated with clinical outcome , and the presence of LOH and DNA alterations of the Rb gene were proved to indicate poor prognosis . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Wild type large T and small t cooperated in stimulating DNA synthesis and in inducing hyperphosphorylation of the Rb gene product ( pRb ) . ^^^ In contrast , a T antigen mutant defective for pRb binding ( Rb T ) possessed no detectable mitogenic activity alone and failed to complement small t in stimulating DNA synthesis . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
This findings was most likely a point mutation or microdeletion because Southern blot analysis of peripheral blood DNA failed to disclose any structural aberration of the RB 1 gene . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The sites within the RB 1 gene where these mutations occur contain interspersed repetitive DNA sequences , direct and inverted repeat sequences and / or dyad symmetrical elements suggesting that these areas promote the appropriate local sequence environment for the generation of deletions and insertions in the RB 1 gene . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Protein kinase C mediated bidirectional regulation of DNA synthesis , RB protein phosphorylation , and cyclin dependent kinases in human vascular endothelial cells . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The molar ratio of platinum bound to nucleotides ( rb ) to produce 50 % of Z DNA reactivity was 0 . 10 for cis DDP : pF 18 , 0 . 15 for Pt pentamidine : pF 18 , and 0 . 10 for cis DDP : pUCZ 8 and Pt pentamidine : pUCZ 8 . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
To prove this , extensive DNA testing was conducted , including determination of intragenic RB 1 DNA sequence polymorphisms and screening for mutation using the polymerase chain reaction coupled with single strand conformation polymorphism analysis . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In DNA binding assays , an NGFI A fusion protein and the native protein product of the NGFI A gene purified from prostate cancer cells bound specifically to an oligonucleotide containing the RB promoter element . ^^^ In summary , the in vitro DNA binding data and the transient coregulation of rat NGFI A and RB following castration suggests that the RB gene may be transcriptionally regulated by NGFI A in prostate cells . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We collected cells of the 327th passage of SO Rb 50 , purified its genomic DNA and detected it with Rb and c myc cDNA probes respectively ( normal human white blood cells DNA was the control ) . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The retinoblastoma ( RB ) tumour suppressor protein is capable of repressing the activity of promoters containing DNA binding sites for the transcription factor E2F . ^^^ Recently a protein which binds RB and possesses the DNA binding characteristics of E2F has been cloned . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The role tumor suppressors p 53 and retinoblastoma ( RB ) play in the transformation process has become central to understanding the pathogenesis of DNA tumor viruses . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Our DNA sequencing data indicate that the promoter region of the p34cdc2 gene contains putative E2F like binding sites which are recognized by Rb and binding sites for c myb , Sp 1 , and ATF , and that the promoter region of the cyclin A gene contains binding sites for p 53 , Sp 1 , and ATF . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We detected DNA aberrations at the RB locus in surgical specimens of human primary non small cell lung carcinoma by single strand conformation polymorphism analysis of polymerase chain reaction products amplified from regions of exons 2 to 27 including parts of the flanking intron sequences . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
It has been proposed that some of the oncogenes of DNA tumor viruses act by binding the products of tumor suppressor genes , such as the retinoblastoma gene product Rb . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
DNA rearrangement was reported to be present in 1 . 5 12 . 1 % of cases with primary leukemias , and the absence of RB protein was also observed in 6 . 3 23 . 2 % . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We have analyzed paired samples of genomic DNA from peripheral leukocyte and primary tumor tissue from nine patients with retinoblastoma ( RB ) and from two RB cell lines , WERI Rb 1 and Y 79 , to detect the molecular alterations of the retinoblastoma susceptibility gene ( RB 1 ) and N myc gene . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We report the isolation and characterization of the mouse RB 1 promoter and surrounding DNA sequences , and the identification of elements required for basal transcriptional activity . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The induced increase in RB protein per cell occurred early , by 8 h of treatment , preceding any significant phenotypic differentiation evidenced by cellular expression of the CD 41 differentiation specific megakaryocytic cell surface marker , but not inhibition of cell cycle transit , leading to a cell population arrested with 2 n , 4 n , and 8 n DNA content . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The effects of the pyridine ligand are further manifested by a greatly enhanced DNA DNA interstrand cross linking efficiency for the trans isomer , with a cross link per adduct frequency of between 0 . 14 and 0 . 23 , depending on the rb of the sample . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Immunopurified Rb protein inhibits SV 40 T antigen dependent stimulation of DNA polymerase alpha . ^^^ Here , we show the effect of Rb protein on the stimulation of DNA polymerase alpha by Tag , in an in vitro system using either purified human or calf thymus DNA polymerase alpha and either primed single stranded M 13 DNA or calf thymus activated DNA . ^^^ Primase activity of DNA polymerase alpha primase complex was also stimulated by Tag , and this stimulation was abolished by the presence of Rb protein . ^^^ Kinetic analysis revealed that in the presence of Tag the apparent Km for the template of either human or calf DNA polymerase alpha was decreased by approximately 2 . 5 fold and the Vmax was increased twofold , whereas Tag complexed with Rb protein did not affect the Km or the Vmax . ^^^ These results suggest a competition between Rb protein and DNA polymerase alpha for binding to Tag , which may be a key step for the initiation of SV 40 DNA replication . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
A novel gene transfer approach was used to investigate whether the retinoblastoma ( Rb ) binding domain of simian virus 40 ( SV 40 ) T antigen is required for efficient T antigen mediated stimulation of DNA synthesis is quiescent or senescent human embryo fibroblasts . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
RB gene structure was studied at the DNA level by Southern blot and chromosome 13 restriction fragment length polymorphism analyses . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Point mutations of RB and p 53 that inhibit their complexing with other DNA viral oncoproteins do not affect their binding to EBNA 5 . p 53 competes with RB for EBNA 5 binding . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
DBcAMP ( 1 mM ) addition , inhibitory on the DNA synthesis , enhanced this down regulation , but did not increase the phosphorylated form of RB protein at the S phase . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Fluorescence activated cell sorting ( FACS ) analysis revealed that cultures induced to overexpress either wild type or Dra mutant Rb proteins were significantly enriched for cells with a G 2 DNA content . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Here we report the isolation of a complementary DNA encoding DRTF 1 polypeptide 1 ( DP 1 ) , a major sequence specific binding protein that is present in DRTF1 / E2F , including Rb and p 107 associated DRTF1 / E2F . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In addition , potent calmodulin antagonists W 7 and calmidazolium , but not an inactive analogue W 12 or W 5 , strongly inhibit serum induced increases in DNA synthesis and RB phosphorylation in IMR 90 and WI 38 cells , whereas in SV WI 38 cells , the inhibitory effect is much more limited . ^^^ In IMR 90 cells that have been incubated with serum in 0 . 03 mM [ Ca ] out for 24 h , restoration of [ Ca ] out to 2 . 0 mM results in initiation of DNA synthesis after 13 h and concomitant increases in RB phosphorylation and p34cdc2 histone H 1 kinase activity . ^^^ These results suggest that in human fibroblasts , Ca2+ / calmodulin regulates the signaling cascade leading to cdc 2 kinase activation , RB protein phosphorylation , and DNA synthesis and that this Ca ( 2+ ) dependent regulation is abrogated in SV 40 transformed cells . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Mutant E 7 proteins defective for RB binding failed to induce the active form of E2F or act synergistically with insulin to stimulate DNA synthesis . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Single strand conformation polymorphism analysis and DNA sequencing were used to detect p 53 point mutations , Northern blotting was used to examine abnormal expression of p 53 and Rb , and polymerase chain reaction and Southern blotting were used to analyze allelic loss . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Examination of the nucleotide sequence of the retinoblastoma ( Rb ) promoter revealed the presence of a DNA region highly homologous to the recognition site for the cellular transcription factor AP 1 . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Induction of susceptibility to tumor necrosis factor by E1A is dependent on binding to either p 300 or p 105 Rb and induction of DNA synthesis . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Multiparameter flow cytometry was used to simultaneously measure nuclear DNA content , RB protein , and MYC or FOS protein per cell . ^^^ Although the amount of RB protein per cell increased with progression through successive cell cycle phases , during S phase the relative rate of increase was not as rapid as that of nuclear DNA . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Wild type but not mutant RB enhanced both binding activity of NF IL 6 to its cognate DNA sequences in vitro and promoter transactivation by NF IL 6 in cells . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
When telomere repeats are critically shortened , DNA damage response pathways involving p 53 ( and in some cell types retinoblastoma protein ) are invoked , leading to `` M 1 senescence ' ' in normal cells ; cancer cells , which frequently lack normal p 53 and RB functions , often develop chromosomal instability leading to telomeric associations , ring chromosomes , and breakage fusion bridge cycles . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Three of the four cases with DNA polymerase beta gene mutation had mutations of the p 16 or RB genes or loss of heterozygosity of the p 53 and APC gene loci . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In vivo administration of transforming growth factor beta 1 , an inhibitor of DNA synthesis in regenerating liver , resulted in reduced expression of Rb as well as its protein partners , cell cycle dependent kinase 4 and cyclin E . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We had found that in an early stage of DNA damage induced , p 53 independent apoptosis , retinoblastoma ( RB ) protein is hypophosphorylated to a p 115 form by an activated serine / threonine phosphatase . ^^^ Here , we report that accompanying the internucleosomal fragmentation of DNA , the newly formed p115 / hypo / RB was immediately cleaved into at least two fragments , p 68 and p 48 . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
DNA from members of this ' low penetrance ' pedigree was subjected to an exon by exon SSCP analysis of the RB 1 gene . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Mutations in the RB 1 promotor region and exons 13 21 of the RB 1 gene were likewise examined by single strand conformation polymorphism ( SSCP ) analyses and DNA sequencing of genomic DNA from 30 microdissected primary pancreatic tumors and the pancreatic carcinoma cell lines . ^^^ Analyses of the RB 1 gene and promotor region by SSCP and DNA sequencing largely confirmed the immunochemical findings . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Cells that have passed beta selection differ from the preceding cells by several criteria , including hyperphosphorylation of Rb , increased expression of cyclins A and B , down regulation of p 27 , increased CDK 2 activity , an induction of cdc 2 activity , and progression through DNA synthesis . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Mutations in E1A that disable it from binding Rb and related pocket proteins have little effect on its ability to stimulate DNA synthesis in cardiac myocytes . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The main cellular factors which are activated by DNA damage and interfere with the cell cycle controls are : p 53 , delaying the transition through the G 1 S boundary ; p21WAF1 / CIP1 , preventing the entrance into S phase ; proliferating cell nuclear antigen ( PCNA ) and replication protein A ( RPA ) , blocking DNA replication ; and the p 53 variant protein p 53 as together with the retinoblastoma protein ( Rb ) , with less defined functions during the G 2 phase of the cell cycle . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Assays for DNA fragmentation revealed lower molecular weight DNA and the apoptosis associated oligo nucleosomal ladder following IFN gamma treatment of the RB defective cells while higher molecular weight DNA was present in the IFN gamma treated , RB reconstituted lines . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The results suggest that the high frequency of chromatid aberrations in the cells from hereditary RB patients results from a genetic deficiency in DNA repair . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We also demonstrate that p16INK4a levels are increased in primary cells in which RB has been inactivated by DNA tumor virus proteins . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The results indicate that saikosaponin a and ginsenoside Rb 1 may enhance DNA repair , and ginsenoside Rb 1 may also have the ability to inactivate the mutagenic activity of AF 2 directly . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
LOH was studied by polymerase chain reaction ( PCR ) of an informative RB DNA polymorphism . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Therefore , mutation of the Rb gene can lead to uncontrolled cell division and thus promotion of transformed cells . p 53 protein will prevent replication of cells with damaged DNA . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
A unique sequence human Rb 1 cosmid DNA probe has been used to localize this region on apes ' Chr 14 by the FISH technique . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We investigated DNA from 45 primary human head and neck cancers to determine LOH at the Rb locus using a polymerase chain reaction based restriction fragment length polymorphism assay . ^^^ These results suggest that inactivation of Rb protein is involved in the carcinogenesis of head and neck cancer at all levels of the process of protein expression : DNA , mRNA and protein . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The data demonstrate that SDI 1 is an important downstream effector of p 53 , but here we report that it can also cause inhibition of DNA synthesis in several immortal human cell lines , independent of p 53 or Rb status . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Expression of these gene products was examined in 69 formalin fixed , paraffin embedded cervical biopsies by immunohistochemistry utilizing antibodies against p 53 , Rb , and proliferating cell nuclear antigen ( PCNA ) and by human papillomavirus DNA in situ hybridization assays . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The Rb pocket was originally defined as the binding site for oncoproteins from DNA tumor viruses such as adenovirus E1a . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Discordance between the DNA and protein data suggests that there may be minor deletions and point mutations in the RB 1 gene or abnormalities in the proteins regulating the expression of pRB . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
One of the 2 nitroimidazole mustards ( 11b ) had 20 fold greater toxicity towards aerobic AA 8 cells than RB 6145 ( 4 ) , and a 51 fold greater toxicity towards UV 4 cells ( which are defective in DNA cross link repair and thus hypersensitive to cross linking agents ) . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Here we present a combination of extensive PCR multiplexing and two dimensional ( 2 D ) DNA electrophoresis to screen for mutations in 26 exons of the retinoblastoma ( RB 1 ) tumor suppressor gene . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Aberrations of the p 53 and Rb tumour suppressor genes were examined in 12 human hepatocellular carcinoma ( HCC ) derived cell lines from different geographic areas and 9 local HCCs by restriction fragment length polymorphisms ( RFLP ) , polymerase chain reaction single strand conformation polymorphisms ( PCR SSCP ) and DNA sequencing . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The far ranging distribution of genes for aromatic hydrocarbon catabolism , predominantly studied in soil pseudomonads , is extended to a marine oligobacterium by finding five homologous sequences in a 5 . 7 kb chromosomal DNA from a new isolate , Cycloclasticus oligotrophus RB 1 . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The RB 1 gene was characterized in 68 tumor DNA samples using Southern analysis and the PG3 . 8M or H 3 8 probes . ^^^ Sixty three tumor DNA samples were analyzed for defects in the RB 1 promoter using polymerase chain reaction ( PCR ) and heteroduplex analysis . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The allele Dna 1 rb determines low DNase 1 inducibility in the kidneys and occurs in DBA / 2 and C57BL / 6 strains . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Rb protein is a critical regulator of entry into the cell cycle , and loss of Rb function by deletions , mutations , or interaction with DNA viral oncoproteins leads to oncogenic transformation . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The same limitation does not apply to a similar ribozyme with DNA arms ( TAT RB ) or to an all RNA ribozyme with only 6 hybridising bases on each arm ( TAT RA 6x2 ) . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Geminivirus DNA replication is reduced in plant cells transfected with plasmids encoding either ZmRb 1 or human p 130 , a member of the Rb family . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
To elucidate the pathogenesis of adenovirus type 12 ( Ad 12 ) induced rat retinal tumor , an experimental animal model of human retinoblastoma ( RB ) , DNA analysis , in situ hybridization and immunohistochemistry were performed . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Exposure of the Rb null parental cell line to 20 microM N hexanoyl D sphingosine for 24 h resulted in a classical pattern of DNA fragmentation that was accompanied by apoptotic nuclear morphological alterations . ^^^ In contrast , the Rb ( + ) clones demonstrated suppression of DNA fragmentation in response to N hexanoyl D sphingosine . ^^^ We conclude that Rb is not required for ceramide induced apoptosis and that Rb can actually inhibit the DNA fragmentation and nuclear morphological changes associated with classical apoptosis . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The polymerase chain reaction and highly informative microsatellite markers were used to compare DNA from matched sets of 63 head and neck squamous cell cancers and normal tissue for LOH at the p 53 and Rb loci . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In screening the leukocyte DNA of three patients with bilateral retinoblastoma for RB 1 gene heterozygous germline mutations , we identified mutations involving exon 3 or 18 of the RB 1 gene by using heteroduplex analysis and sequencing . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
These results thus provide a direct link between the initiation of DNA replication and the cell growth regulatory pathway involving G 1 cyclin dependent kinases , the Rb tumor suppressor , and E2F . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The three proteins share a region known as the pocket that is important for binding E2F and is also the binding site for oncoproteins from DNA tumor viruses that inactivate Rb . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Wild type Rb also enhanced the binding of C / EBP to cognate DNA sequences in vitro and the transactivation of a C / EBPbeta responsive promoter in cells . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
RBlox / RB / mutant fetuses die at birth with specific skeletal muscle defects , including increased cell death prior to myoblast fusion , shorter myotubes with fewer myofibrils , reduced muscle fibers , accumulation of elongated nuclei that actively synthesized DNA within the myotubes , and reduction in expression of the late muscle specific genes MCK and MRF 4 . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Apoptosis was shown to be induced by expression of DNA tumor virus oncoproteins , knockout of the rb gene , and expression of E2F from heterologous promoter . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The retinoblastoma protein ( RB ) regulates the cell cycle by binding and inactivating the E2F transcription factors , which prevents transcription of genes required for DNA synthesis . ^^^ Our results indicated that cotreating the RB defective S 4 cells with IFN gamma and Vitamin D 3 decreased the number of cells containing subdiploid DNA compared to cells treated with IFN gamma alone , suggesting that Vitamin D 3 reduced IFN gamma mediated apoptosis . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In an attempt to characterize the molecular alterations of cervical adenocarcinoma , we analyzed 32 paraffin embedded specimens for the presence of K ras mutations , p 53 overexpression , p 16 and Rb protein expression , and the presence of HPV 16 and 18 DNA . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In order to determine whether E2F 1 that can not bind to RB might be associated with various human cancers , we searched for mutations in the RB binding domain of E2F 1 using samples of DNA from various clinical specimens obtained from 406 cancer patients ( with lung , pancreatic , stomach , colon , esophageal , and hepatic cancers ) by analysis of polymerase chain reaction mediated single strand conformational polymorphism . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The function of p 53 in human RB cell lines was tested by transfecting them with a complementary DNA encoding a temperature sensitive isoform of murine p 53 under the control of a strong viral promoter . ^^^ RESULTS : DNA from RB tumor specimens showed a strong nucleosomal ladder of DNA fragments typical of apoptosis . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
To better define the incidence of 13q deletions and trisomy 12 , FISH analysis was also performed using a cosmid probe that recognized a DNA sequence between the Rb gene and the D13S25 locus at band 13q14 and a chromosome 12 specific pericentromeric probe . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
E1A lacking the capacity to interact with Rb , but capable of p300 / CBP interaction , was competent in suppression of a DNA damage activated p 53 dependent cell cycle checkpoint . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
DNA probes for 13q12 . 3 ( BRCA 2 ) and 13q14 ( RB 1 and DBM locus ) were used in 35 cases . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The Rb family proteins associate with the factors of the E2F family to form transcription repressor complexes , which control expression of several genes essential for S phase entry and DNA replication . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
RB appears thus to control directly the balance between DNA and protein synthesis during the cell cycle . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Small DNA tumour viruses , such as simian virus 40 ( SV 40 ) , papilloma viruses and adenoviruses , encode proteins that form complexes with and inactivate the p 53 and retinoblastoma ( RB ) proteins . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In contrast , IFN alpha did not affect the DNA binding ability of E2F 1 and E2F 4 in a complex with retinoblastoma ( RB ) susceptibility gene family proteins including pRB , p 107 , and p 130 . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Experiments utilizing Gal 4 RB and Gal 4 p107 chimeric constructs demonstrated that either RB or p 107 could directly repress TGF beta induction of p 15 ( INK4B ) gene when tethered to p 15 ( INK4B ) promoter through Gal 4 DNA binding sites . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
For the cell cycle analysis , HL 60 human promyelocytic leukemia cells were fluorescently stained for DNA and for the RB protein . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Reducing agents , N acetylcysteine , reduced glutathione or 2 ME restored both RB phosphorylation and DNA synthesis in thiol deprived NK3 . 3 cells . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Extensive DNA methylation spanning the Rb promoter in retinoblastoma tumors . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
A simian virus 40 ( SV 40 ) T antigen mutant containing only the N terminal 136 amino acids , able to bind to Rb and p 300 but not p 53 , partially inhibited p 53 mediated transcription without affecting the ability of p 53 to bind DNA . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Observed similarities to interactions of other DNA virus proteins ( human papillomavirus type 16 E 6 and E 7 , simian virus 40 large T antigen , and adenovirus type 5 E1A and E1B ) with cell cycle modulatory proteins such as p 53 and Rb have suggested that HCMV IE proteins may likewise alter the G 1 to S phase transition . ^^^ Induction of DNA damage in normal , uninfected fibroblasts ( FB ) or FB expressing IE 86 by actinomycin D ( Act D ) resulted in increased p 53 levels , a predominance of the hypophosphorylated form of Rb , and increased expression of both p 21 ( CIP1 / WAF1 ) and mdm 2 . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Binding to the proteins Rb 1 and p 107 in vitro and induction of DNA synthesis after microinjection into serum deprived NIH 3T3 cells suggest that the E 7 protein retains some of its biological activities . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We conclude that 1 ) mitogenic stimulation of cultured bovine tracheal myocytes by PDGF induces cyclin D 1 transcriptional activation and protein expression , 2 ) cyclin D 1 expression is accompanied by Rb phosphorylation , which is evidence of increased cyclin D 1 associated kinase activity in vivo , and 3 ) microinjection of anti cyclin D 1 antibodies inhibits cellular DNA synthesis , which is evidence that cyclin D 1 is required for airway smooth muscle S phase traversal . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
By mutation analysis in DNA from fresh frozen tumor samples and peripheral blood , we have detected RB 1 germline mutations in some 20 % of patients with unilateral RB . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Taken together , these data suggest a novel interaction of Rb with the recombinase REC 2 and a role for this complex in bridging DNA recombination and apoptosis . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We analyzed DNA from a variety of tissues and specifically targeted CGA codons in RB 1 , where recurrent premature termination mutations have been reported . ^^^ We found that DNA methylation within RB 1 exons 8 , 14 , 25 , and 27 appeared to be restricted to CpGs , including six CGA codons . ^^^ Therefore , disease causing mutations at CpGs in RB 1 appear to be determined by several factors , including the constitutive presence of DNA methylation at cytosines within CpGs , the specific codon within which the methylated cytosine is located , and the particular region of the gene within which that codon resides . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Although the phosphorylation sites were involved in viral DNA replication , none was important for the ability of large T to drive cellular DNA replication as measured by bromodeoxyuridine incorporation , and they did not affect large T interactions with the Rb tumor suppressor family . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The cloning of the gene responsible for retinoblastoma ( Rb 1 ) has facilitated DNA studies and genetic counselling of patients . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Rb E2F complexes suppress transcription of genes required for DNA synthesis ( [ 4 ] , reviewed in [ 3 , 5 ] ) , and the prevailing view is that phosphorylation of Rb by complexes of cyclin dependent kinases ( Cdks ) and their regulatory cyclin subunits , and the subsequent release of active E2F , is required for S phase entry [ 1 3 ] . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We have observed previously that the introduction of a transcription unit containing an antisense sequence for the E6 / E7 genes of human papillomavirus ( HPV ) 18 , along with a transcription unit containing a sense complementary DNA sequence for the wild type retinoblastoma ( Rb ) gene , decreased the growth of human cervical carcinoma HeLa cells ( HPV 18 positive ) both in vitro and in vivo . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We have used our knowledge of this model to analyze control ( sham treated ) and irradiated renal tissue for Rb mRNA transcript levels and localization ( Northern blot and in situ hybridization ( ISH ) ) , pRb expression ( Western blot and immunolocalization ) , apoptosis and mitosis ( light and electron microscopy , and DNA gel electrophoresis for apoptosis ) , and cells in S phase ( [ 3H ] thymidine uptake and autoradiography ) . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
To determine the frequency and nature of constitutional RB 1 gene mutations in patients with isolated unilateral retinoblastoma , we analyzed DNA from peripheral blood and from tumor tissue . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Transfection of immortal retinoblastoma cells with DNA sequences encoding wild type p 53 protein induces cell death , suggesting that the loss of both RB 1 and p 53 functions may be required for cell immortalization . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
These changes were accompanied by a decrease in E2F DNA binding and the accumulation of the hypophosphorylated form of Rb . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
OBJECTIVE : Paired DNA samples from 55 primary uterine cervical carcinomas and normal bloods were studied for chromosomal allelic loss ( loss of heterozygosity ; LOH ) of the retinoblastoma ( Rb ) and p 53 gene loci by polymerase chain reaction ( PCR ) or PCR restriction fragment length polymorphism analysis . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Using recombinant E2F , DP , and Rb proteins prepared in baculovirus infected cells and a repetitive immunoprecipitation PCR procedure ( CASTing ) , we have identified consensus DNA binding sites for E2F 1 / DP 1 , E2F 1 / DP 2 , E2F 4 / DP 1 , and E2F 4 / DP 2 complexes as well as an Rb / E2F 1 / DP 1 trimeric complex . ^^^ Our data indicate that ( 1 ) E2F , DP , and Rb proteins each influence the selection of E2F binding sites ; ( 2 ) E2F sites differ with respect to their intrinsic DNA bending properties ; ( 3 ) E2F / DP complexes induce distinct degrees of DNA bending ; and ( 4 ) complex specific E2F sites selected in vitro function distinctly as regulators of cell cycle dependent transcription in vivo . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Further , the use of DNA testing in children may be able to identify the presence of germline RB and p 53 mutations , which may identify a child at high risk for SMN , so that appropriate therapeutic modifications may be made . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The small GTPase RhoB is immediate early inducible by DNA damaging treatments and thus part of the early response of eukaryotic cells to genotoxic stress . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Human retinoblastoma ( Rb ) protein , immunopurified from an extract of recombinant baculovirus infected cells , stimulated 10 100 fold the activity of DNA polymerase alpha from calf thymus or human HeLa cells . ^^^ On the other hand , a hyperphosphorylated Rb protein , obtained from insect cells overexpressing Rb protein , cyclin E and cyclin dependent kinase 2 simultaneously , stimulated DNA polymerase alpha more strongly than the singly expressed Rb protein . ^^^ Rb protein isolated from human Burkitt lymphoma Raji cells also stimulated DNA polymerase alpha . ^^^ In contrast , Rb protein did not affect eukaryotic DNA primase or Klenow fragment of Escherichia coli DNA polymerase 1 . ^^^ By immunoprecipitation using anti DNA polymerase alpha antibody , Rb protein in nuclear extract of Raji cells was co precipitated with DNA polymerase alpha . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The biological state of the tumour suppressor proteins Rb and p 53 is altered in papillomavirus and SV 40 transformed cells , due to interaction with the DNA tumour virus oncogene proteins E6 / E7 and the tumour ( T ) antigen . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The RB 1 gene mutation was investigated in a child with ectopic intracranial retinoblastoma using DNA obtained from both the pineal and retinal tumours of the patient . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Supershift analysis utilizing epitope specific Rb monoclonal antibodies indicated the presence of Rb protein bound to the RCE containing DNA fragment . ^^^ In contrast , polyclonal anti Rb antibodies enhanced the amounts of nuclear protein DNA complexes detected but did not result in a supershift . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Calu 1 non small cell lung cancer cells , which retain Rb and lack p 53 , do not arrest in G 1 following DNA damage . ^^^ DNA damage induced G 1 arrest , which requires the expression of pocket proteins such as Rb , can be abrogated by overexpression of cdk 4 , kinase inactive cdk 4 variants capable of sequestering p16INK4A , or a cdk 4 variant incapable of binding p16INK4A . ^^^ Nonetheless , p16INK4A expression is required for the reduction in cdk 4 and cdk 6 mediated Rb kinase activity observed in response to DNA damage . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Utilizing genomic DNA isolated from the tumour samples and control normal cells , 2 highly polymorphic microsatellite loci ( D13S153 and Rb 1 . 20 ) located within the Rb gene were PCR amplified and examined for loss of heterozygosity . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
These two G 1 cyclin Cdk complexes act on a family of E2F associated transcriptional repressors typified by the retinoblastoma protein ( Rb ) to bring about a transcriptional program that promotes passage through S phase [ 7 9 ] , but can also activate DNA replication independently of Rb E2F [ 10 12 ] . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Despite having 4N DNA content , adapted cells are similar to G 1 cells in that they have upregulated cyclin E expression and hypophosphorylated Rb protein . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
It is found that a rapid twofold elevation of ouabain sensitive Rb influx in response to PHA is followed by a long term increase in pump activity , which precedes the DNA synthesis and is temporally related to the growth phase of mitogenic response . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In addition , one untreated stage C , grade 9 specimen was found to express RB 1 transcripts lacking exon 22 , as determined by sequencing of DNA from the truncated transcripts . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Accompanied by the internucleosomal fragmentation of DNA , the newly formed p115 / hypo / RB is immediately cleaved by a protease that has properties of the caspase family . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The studies show that full length T antigen with point mutations to reduce binding to the cellular proteins p 53 and / or Rb were capable of inducing > G 2 DNA content though the induction by these mutants was greatly enhanced by the presence of the SV 40 origin of replication . ^^^ Truncated T antigen ( aa 1 259 ) could induce cells with tetraploid DNA content only in the presence of the SV 40 origin of replication and the absence of Rb binding . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The amino termini of Rb and p 130 strongly inhibited DNA replication in an MCM 7 dependent fashion in a Xenopus in vitro DNA replication assay system . ^^^ These data provide the first evidence that Rb and Rb related proteins can directly regulate DNA replication and that components of licensing factor are targets of the products of tumor suppressor genes . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Compared to cogenic wild type mice , p 21 / mice demonstrated evidence of markedly accelerated hepatocyte progression through G 1 phase after PH : DNA synthesis , upregulation of cyclin A and PCNA , induction of cyclin D 1 and CDK 2 associated kinase activity , and appearance of a phosphorylated retinoblastoma protein ( Rb ) species occurred earlier in the p 21 / mice . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
AIAT revealed that apoptosis index ( AI ) in regressed areas was higher than that in other areas of Rb , DNA average ploidy ( DP ) consisted with the histology grade of Rb , the degree of hyperdiploid ( DH ) in metastatic area was distinctly higher than that in other areas ( P < 0 . 05 ) . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
By the use of DNA probes from the hamster dhfr gene promoter , containing the most proximal GC box ( minimal promoter ) , and nuclear extracts from cultured hamster cells ( CHO K 1 ) , we show that polyclonal and monoclonal antibodies against Rb supershift the binding of Sp 1 . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The role of Rb gene for SMC proliferation , DNA synthesis and cell cycle were observed by cell counting . 3H incorporation and cytometer respectively . ^^^ The expression of Rb gene restricted the proliferation of SMC , decreased the DNA synthesis and plaaied a role in the cell cycle . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
During HCMV infection , one of its proteins interacts with a cell growth regulatory protein , the retinoblastoma protein ( Rb ) and stimulates DNA synthesis which is associated with chromosomal damage and cellular mitotic arrest . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We now show that UV irradiation of B 16 melanoma after prior exposure to the radiation sensitizer , bromodeoxyuridine ( BUdR ) leads to induction of p 53 and DNA fragmentation , and concomitant decreases in Rb , E2F , cyclin D 1 , and cell viability , with no comparable effects on irradiated unsensitized cells . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We evaluated the usefulness of fluorescence in situ hybridization ( FISH ) using different colored commercial RB 1 and 13qter DNA probes to identify RB 1 deletions in interphase nuclei of bone marrow from 24 patients with agnogenic myeloid metaplasia ( AMM ) , 20 patients with multiple myeloma ( MM ) , 21 patients with other hematologic malignancies , and 25 normal bone marrow transplant ( BMT ) donors . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Recruitment of Rb to promoters , by association with E2F complex or by fusion with heterologous DNA binding domains , demonstrated that Rb represses directly transcription . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The yeast derived Flp frt site specific DNA recombination system was used to achieve pituitary specific inactivation of the retinoblastoma ( Rb ) tumor suppressor gene . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Simian virus 40 ( SV 40 ) has been shown to induce brain tumors , osteosarcoma , lymphoid tumors and malignant mesothelioma in hamsters and SV 40 like DNA sequences corresponding to the Rb pocket binding domain of SV 40 T antigen ( Tag ) have been detected in the same human tumors . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Inhibition of DNA synthesis by RB : effects on G1 / S transition and S phase progression . ^^^ Interestingly , Rat 1 cells coexpressing cyclin E and PSM RB could not complete DNA replication . ^^^ Microinjection of cells that have passed through the G 1 restriction point with plasmids expressing PSM RB also led to the inhibition of DNA synthesis . ^^^ These results reveal a novel role for RB in the inhibition of S phase progression that is distinct from the inhibition of the G1 / S transition , and suggest that continued phosphorylation of RB beyond G1 / S is required for the completion of DNA replication . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Interestingly , contrary to the prevailing theory that CDK 4 amplification is an alternative mechanism for RB gene mutation , we found that three of four cases with amplification of the CDK 4 gene showed loss of expression of the RB protein , one of which was proved to have an gross DNA alteration in the RB locus . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
METHODS : All identifiable reported cases of bilateral retinoblastoma , which included DNA sequence analysis of the RB gene , were reviewed . ^^^ RESULTS : Among 192 patients with retinoblastoma with identifiable germline mutations in the RB gene , the DNA alteration was a nonsense mutation in 83 ( 43 % ) , frameshift in 67 ( 35 % ) , intron mutation in 23 ( 12 % ) , missense mutation in 11 ( 6 % ) , in frame deletion in 5 ( 3 % ) , and promoter mutation in 3 ( 2 % ) . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The transcription of a number of these genes , including E2F1 and B myb , is repressed in G0 / early G 1 at E2F DNA binding sites mediated by interaction of E2F with the Rb family member proteins . ^^^ Moreover , it was shown that a factor specifically bound to the CHR element is co purified with E2F by DNA affinity purification and co immunoprecipitated with E2F4 and the Rb family members . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Retinoblastoma protein ( Rb ) is a key regulator of cellular proliferation , controlling entry into G1 / S in the cell cycle , largely through its action in binding the cellular transcription factor E2F , which activates genes important in DNA synthesis . ^^^ Small DNA tumor viruses encode gene products which can functionally inactivate Rb , promoting cellular proliferation and viral DNA synthesis . ^^^ The interaction of R and Rb may initiate cell cycle progression and facilitate viral DNA synthesis during lytic replication . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Rb represses S phase genes through the recruitment of an enzyme which modifies DNA structure , the histone deacetylase HDAC 1 . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
To this end , cyclin D 1 gene amplification and protein accumulation , Rb gene allelic loss and protein expression , and MTS 1 gene mutation and DNA methylation were investigated in a series of 74 esophageal carcinomas . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
RESULTS : Wild type Rb gene transfected effectively into the cultured SMC with AdCMVRb can suppress growth factor stimulated cell proliferation through regulation of DNA synthesis and cell cycle progression . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
TGF beta 1 inhibited the increases in ODC activity , RB phosphorylation , and DNA synthesis induced by HGF . ^^^ However , polyamines added exogenously could not overcome the inhibition by RB phosphorylation and DNA synthesis by TGF beta 1 . ^^^ These results suggest that polyamines are involved in the phosphorylation of RB , but the inhibition of polyamine biosynthesis by TGF beta 1 did not result in the inhibition of RB phosphorylation and DNA synthesis . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The possible alarm signals include synthesis of poly ( ADP ribose ) polymers and initiation of phosphorylation cascades by kinases complexed with the DNA damage sensors , DNA PK and ATM ; the role of other factors is discussed , among them BRCA 1 and 2 , IRF 1 and RB ( retinoblastoma ) . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Unexpectedly , correlation of these findings with our prior studies indicated that five of ten osteosarcomas positive for SV 40 DNA had mutations of p 53 , and two had deleted Rb . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Previously , we had found that during DNA damage induced apoptosis , retinoblastoma protein ( RB ) , an important G1 / S regulator and tumor suppressor , became dephosphorylated and then immediately cleaved into p 48 and p 68 fragments . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We conclude that activating mutations of ras , loss of DNA from the Rb locus and over expression of both c met and bFGF are of no pathogenetic relevance in driving thyroid tumorigenesis of iodine deficient areas . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The RhoB binding domain in DB 1 was located in a functionally undefined region upstream and separable from its zinc finger DNA binding domain . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The data suggests that abnormalities in the function of Rb and E2F 1 proteins may also be responsible for the failure of AT cells to arrest in the G1 / S checkpoint in response to DNA damage . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
These new markers include DNA ploidy , S phase , certain monoclonal antibodies , the p 53 ( alias TP 53 ) tumor suppressor gene , the retinoblastoma ( Rb ) gene , cell adhesion molecules , and angiogenesis . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Since Rb and factor 8 genes have displayed high frequency of chromosomal deletions in various cancers and haemophilia A , the universal integration of long and homologous L 1 segments in the genes and all chromosomes may be liable to promote abnormal DNA rearrangement . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
RB 1 gene mutations in peripheral blood DNA of patients with isolated unilateral retinoblastoma . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Anchorage dependent expression of cyclin A in primary cells requires a negative DNA regulatory element and a functional Rb . ^^^ In the experiments reported here , we show that anchorage dependent expression of cyclin A ( 1 ) is reflected by the in vivo occupancy of a negative DNA regulatory element previously shown to be instrumental in the down regulation of cyclin A transcription in quiescent cells ( Cell Cycle Responsive Element : CCRE ) ( 2 ) requires a functional Rb but neither p 107 nor p 130 ( 3 ) mutation of the CCRE abolishes both adhesion dependent regulation and response to Rb . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Of particular clinical relevance , the frequency of lung metastases also was reduced to 12 % in Rb+ / mice by repeated i . v . administration of lipid entrapped , polycation condensed RB complementary DNA . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Large T antigen ( LT ) expressed by the oncogenic DNA virus SV 40 transforms cells by interacting with and perturbing the normal function of several important cellular proteins including P 53 , RB , c MYC , and AP 2 . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Rb proteins control cell cycle progression by sequestering transcription factors required for entry into S phase , suggesting that the virus modifies the cellular environment to produce conditions suitable for viral DNA replication . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Analysis of proviral DNA synthesis by means of PCR has shown that RB 2121 acts at an early step of the retrovirus life cycle by inducing a dose dependent reduction in transcribed DNA levels through inhibition of NCp 7 reverse transcriptase interaction . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
However , expression of an Rb N terminal oligomerization domain deletion construct , a C terminal DNA binding domain deletion construct , or a pocket domain mutant failed to downregulate telomerase activity . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The retinoblastoma ( RB ) protein exerts its tumour suppressor function by repressing the transcription of cellular genes required for DNA replication and cell division . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Hypophosphorylated retinoblastoma ( Rb ) gene product binds critical transcription factors , leading to G 1 arrest in a number of conditions , including following DNA damage . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The melting temperature of DNA adducted by BBR 3464 increased at low ionic strength but decreased in high salt for the same rb . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In contrast the decreases in pRB expression in response to DNA damage involve transcriptional downregulation of RB gene expression . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
DESIGN AND METHODS : One hundred and twenty five NHL were analyzed by cytogenetics and by interphase fluorescence in situ hybridization ( FISH ) , using a 13q14 cosmid probe recognizing DNA sequences between the Rb gene and the D13S25 marker . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Consistent with reduced levels of Oct 1 DNA binding activity in the Rb transformed cell lines , interleukin 8 expression is higher in these cell lines . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
DNA fingerprinting showed that these cell lines were not contaminants , but derivatives of the original RB cells . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Similarly , transfection of antisense Rb ODN alone resulted in a higher DNA synthesis rate than control ( P < 0 . 01 ) . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Rb binding by E2F 1 was neither necessary nor sufficient for G 1 exit , whereas DNA binding was required ; thus , exogenous E2F 1 did not merely function by competing for the Rb `` pocket . ' ' E2F 1 induced G 1 exit was blocked by the `` universal ' ' Cdk inhibitor p 21 but not by p 16 , a specific inhibitor of Cdk4 / 6 ; p 21 was permissive for E2F 1 induction of cyclins E and A , but prevented their stimulation of Cdk 2 kinase activity . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In total , 11 / 12 MPNSTs showed DNA changes in one or more of the genes CDKN2A , CDKN2B , RB 1 , CDK 4 , MDM 2 , and CCND 2 . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
E2F 1 , a transcription factor implicated in the activation of genes required for S phase such as DNA pol alpha , is regulated by interactions with Rb and by cell cycle dependent alterations in E2F 1 abundance . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In contrast to MB , photodynamic treatment with RB , UP or AIPcS 4 did not lead to DNA damage . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In accordance with these observations , we found that BRCA 1 could not suppress BrdUrd uptake in primary fibroblasts from rb / mice and exhibited an intermediate ability to inhibit DNA synthesis in rb+ / as compared with rb+ / + cells . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In primary cultures of dog thyroid epithelial cells , kinetic experiments indicate that a labile cAMP dependent event positively controls a late G 1 commitment to DNA replication and RB phosphorylation . ^^^ This implies that even after induction of stable nuclear cyclin D 3 CDK4 complexes , dog thyrocytes still depend on cAMP for RB phosphorylation and commitment to DNA synthesis , which suggests that a key labile event responsible for a last control of restriction point passage remains to be uncovered , in the cAMP dependent cell cycle of dog thyrocytes and possibly other systems . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We have used SSCP analysis to study the 27 individual exons of the RB 1 gene in constitutional DNA from 3 patients whose tumours were treated under the age of 12 months . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The distal segment of the mitogenic signaling pathway , which consists of Rb phosphorylation , the release of E2F , the induction of c myc , cyclins E and A , and DNA synthesis , is distinguished by a multiplicity of nested positive feedback loops ; these would be expected to drive a mitogenic signal that entered the distal segment through at least one round of DNA synthesis . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
These findings indicate that caspase 3 induces DNA fragmentation and cleavage of Rb and PARP during the process of apoptosis induced by concanamycin A . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
RESULTS : Small deletions , insertions and whole exon deletion in the RB 1 gene were detected from genomic DNA . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The decrease in RB expression and phosphorylation , which is essential in triggering DNA synthesis and Cyclin A expression , leads to a deficiency in transcriptionally active E2F complex formation after hepatectomy . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The impact of human papilloma virus ( HPV 16 ) E 7 proteins and retinoblastoma ( RB ) antisense oligonucleotides upon mitogen activated protein kinase ( MAPK ) mediated inhibition of DNA synthesis via p 21 ( Cip 1 / WAF1 / MDA6 ) ( p 21 ) was determined in primary hepatocytes . ^^^ Either transfection with RB antisense oligonucleotides , expression of wild type E 7 , or RB binding mutant E 7 ( C24S ) proteins increased p 21 levels and reduced DNA synthesis in p 21 ( + / + ) hepatocytes . ^^^ Antisense ablation of RB in p 21 ( / ) hepatocytes had a weak stimulatory effect upon DNA synthesis itself but enhanced the capacity of mutant E 7 protein to stimulate DNA synthesis to the same level observed using wild type E 7 . ^^^ The ability of prolonged MAPK activation to stimulate DNA synthesis in the presence of mutant E 7 and antisense RB was additive . ^^^ Collectively , the present data demonstrate that loss of RB function together with loss of p 21 function plays an important role in the E 7 and MAPK dependent modulation of apoptosis and DNA synthesis in primary hepatocytes . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
METHODS : Genomic DNA was used as a template for the PCR reaction to amplify all exons as well as the promoter region of RB 1 gene . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Mitogenic signaling events involving cyclins D and E , Rb phosphorylation , and transcriptional activation of E2F responsive genes ( including cyclin E and cyclin A ) were unaffected in cells containing PAH damaged DNA . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
First , the analysis of phosphorylation status of Rb protein and the measurement of the proportion of tumor cells in S phase indicated that Bcl 2 overexpression results in a decrease of DNA synthesis induced by estradiol . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Paired normal and tumor DNA from 6 parathyroid adenomas and 5 aggressive tumors were analyzed for loss of heterozygosity ( LOH ) at the RB locus . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Furthermore , overexpression of RhoB resulted in a prolonged transient block to DNA replication upon MMS treatment . ^^^ Based on the data we suggest RhoB to be a novel regulatory factor which takes influence on the level of cytotoxicity of DNA damaging drugs and forces cells to alkylation induced apoptosis . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The expression of either RB or RBaK in 10T1 / 2 fibroblasts represses the activation of an E2F dependent promoter and decreases DNA synthesis to a similar degree . ^^^ However , a mutant form of RBaK that can not interact with RB in vitro is unable to prevent DNA synthesis . ^^^ We present a model in which RB physically interacts with the novel transcriptional repressor RBaK to repress E2F dependent genes and prevent DNA synthesis . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The DNA sequence encoding a streptavidin recognition motif , or StrepTag ( AWRHPQFGG ) , was cloned and expressed at the C terminus of a fusion protein of a 152 amino acid hyperphosphorylation domain ( Rb 152 ) of the retinoblastoma protein ( Rb ) linked to GST at the N terminus . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
DNA from the promoter region of 11 patients with no / low RB expression was subjected to bisulfite conversion and PCR sequencing . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
D cyclin cdk activity is required for Rb phosphorylation in 5 Jun transformed cells , since ectopic expression of the cdk 4 and cdk 6 specific inhibitor p 16 ( INK4A ) inhibits both DNA synthesis and cell proliferation . ^^^ In particular , hormonal activation of a conditional 5 Jun estrogen receptor fusion protein in quiescent , growth factor deprived cells stimulates cyclin E cdk 2 activity and triggers Rb phosphorylation and DNA synthesis . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
To investigate cell death in retinoblastoma ( Rb ) , ultrastructural examination , ApopTag staining , electrophoresis to detect apoptotic DNA fragmentation , and flow cytometric studies were performed . ^^^ Analysis of DNA fragmentation using 3 ' end labeling with terminal transferase indicated that undifferentiated Rb has increased DNA cleavage . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Polymerase chain reaction ( PCR ) , using primers that amplify the RB 1 pocket binding domain and the intron of Tag , was used to analyze GCT for the presence of SV 40 DNA . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We have characterized two polyomavirus large T antigen mutants with different properties in viral DNA replication . dl 97 , a mutant active in immortalization , exerts a dominant negative effect in viral DNA replication . 13val , which is defective in both immortalization and viral DNA replication , has a lesion in the putative DnaJ domain affecting the block of Rb function . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We show that the inactivation of Rb and p 21 through the binding of the adenovirus E1A protein leads to the induction of DNA replication in differentiated muscle cells . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We examined the expression levels of the p 16 , p 15 , p 14 , and retinoblastoma susceptibility ( RB ) genes in primary prostate cancers and human prostate cancer cell lines , and correlated this with the DNA methylation levels of two loci in p 16 . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Retinoblastoma susceptibility protein , Rb , possesses multiple BRCT Ws , BRCA 1 carboxyl terminus related W regions with DNA break binding activity . ^^^ A BRCT related region , BRCT W 1 , in the retinoblastoma susceptibility gene product ( Rb ) also could bind DNA fragments , independently of DNA sequences . ^^^ All BRCT Ws of Rb bound DNA fragments . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Although the use of a specific caspase 3 like inhibitor ( z DEBD . fmk ) delays RB cleavage and reduces DNA fragmentation , data implicate other caspases in these processes . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
First ; p16INK4 Cyclin D 1 RB pathway , which controls G 1 to S progression of the cell cycle , second ; p 53 pathway , which is involved in DNA damage repair , and third ; p27KIP1 CDK inhibitor , a negative regulator of cell cycle , and decreased expression of which has been correlated to poor prognosis in cancer patients . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
HBeAg seroconversion occurred in 50 . 0 % of the patients in the Rb group ( vs 6 . 6 % in the Pl group ; P = 0 . 00019 ) ; HBV DNA negativization occurred in 33 . 3 % in the Rb group ( vs 6 . 6 % in the Pl group ; P = 0 . 009 ) ; and improvement in the necroinflammatory index occurred in 53 . 3 % in the Rb group ( vs 23 . 3 % in the Pl group ; P = 0 . 02 ) . ^^^ Ribavirin was an effective treatment , demonstrated by decreased ALT levels , alleviation of histological damage , seroconversion of HBeAg , and HBV DNA negativization ; Rb may be an alternative agent in the treatment of CHB , without significant side effects . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Infection of cells with SV 40 , a DNA tumor virus known to abrogate formation of p 130 and Rb containing complexes , allowed dense cultures to proliferate in the presence of supraphysiological amounts of p 27 ( kip 1 ) but did not stimulate cell cycle traverse when cultures were cotreated with the potent cdk 2 inhibitor roscovitine . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Five days later , the cultures were analyzed for cell survival , activation , DNA synthesis , and expression of EBV encoded and of cellular regulatory proteins retinoblastoma ( Rb ) , p 53 , recombinant sequence binding protein ( RBP ) Jk , and PU . 1 . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Here we show that the predominant mammalian DNA methyltransferase , DNMT 1 , co purifies with the retinoblastoma ( Rb ) tumour suppressor gene product , E2F1 , and HDAC 1 and that DNMT 1 cooperates with Rb to repress transcription from promoters containing E2F binding sites . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
By contrast , differentiated myocytes with RB ( N757F ) undergo DNA synthesis and phosphorylate RB ( N757F ) in response to serum , despite a high level of p21Cip1 expression . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
BRCA 1 contains several functional domains that interact directly or indirectly with a variety of molecules , including tumor suppressors ( p 53 , RB , BRCA 2 and ATM ) , oncogenes ( c Myc , casein kinase 2 and E2F ) , DNA damage repair proteins ( RAD 50 and RAD 51 ) , cell cycle regulators ( cyclins and cyclin dependent kinases ) , transcriptional activators and repressors ( RNA polymerase 2 , RHA , histone deacetylase complex and CtIP ) and others . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Mitogen dependent , cyclin D dependent kinases ( cdk 4 and cdk 6 ) phosphorylate the retinoblastoma ( Rb ) tumor suppressor protein , helping to cancel its growth inhibitory effects and enabling E2F transcription factors to activate genes required for entry into the DNA synthetic phase ( S ) of the cell division cycle . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
OBJECTIVE : To investigate the DNA sequence of the 5 ' untranslated region of the Rb gene in normal individuals and retinoblastoma patients and identify the elements involved in transcription regulation of Rb gene . ^^^ The different DNA fragments located in the 5 ' untranslated region of Rb gene were amplified and inserted into an expression plasmid with a CAT reporter gene . ^^^ CONCLUSION : The promoter DNA sequence of Rb gene is quite stable . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In the early phase of adipocyte differentiation , transient increase of DNA synthesis , called clonal expansion , and transient hyperphosphorylation of retinoblastoma protein ( Rb ) are observed . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
When dominant negative mutants at the Rb binding motif were transfected in Rb+ / + MEF , p 204 lost its ability to inhibit cell growth , delay cell transition from G 1 to S phase , and impair DNA synthesis . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Oncoproteins from DNA tumor viruses such as adenovirus E1a , simian virus 40 T antigen , and human papillomavirus E 7 contain an LXCXE sequence , which they use to bind the retinoblastoma protein ( Rb ) and inhibit its function . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
These experiments indicated that repression of E2F responsive promoters following HPV E6 / E7 repression was mediated by activation of the Rb tumor suppressor pathway and the assembly of repressing E2F4 Rb DNA binding complexes . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Fluorescence in situ hybridisation with a biotin labelled oligonucleotide , ( TTAGGG ) 7 , was performed to localise the telomeric DNA sequences at Rb chromosomes of heterozygous shrews . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
RB dependent S phase response to DNA damage . ^^^ In this report , RB is shown to also be required for an intra S phase response to DNA damage . ^^^ Dephosphorylation of RB in S phase cells temporally preceded the inhibition of DNA synthesis . ^^^ This S phase dephosphorylation of RB and subsequent inhibition of DNA replication was observed in p 21 ( Cip 1 ) deficient cells . ^^^ The induction of the RB dependent intra S phase arrest persisted for days and correlated with a protection against DNA damage induced cell death . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Fluorescence in situ hybridization ( FISH ) analysis of dissociated nuclei with DNA probes for MYC ( 8q24 ) / # 8 , cyclin D 1 gene ( CCND 1 ; 11q13 ) / # 11 , ERBB 2 ( 17q13 ) / # 17 , the androgen receptor gene ( AR ; Xq 12 ) / # 10 , and the retinoblastoma gene ( RB ; 13q14 ) was applied to formalin fixed tissue from 63 patients with advanced PC after androgen deprivation therapy ( ADT ) ; matched tumor tissue before ADT was also available in 22 of these cases . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
DNA sequences up to 6 kilobase pairs upstream of the mouse Rb promoter , isolated from two different genomic libraries , directed transgene expression exclusively to the developing nervous system , excluding skeletal muscles and liver . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
DNase footprinting and band shift assays demonstrated that the interaction between Rb and UBF does not inhibit the binding of UBF to DNA . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Singlet oxygen , but not the RB derived radicals , induced nuclear condensation and DNA fragmentation into nucleosome size fragments in a dose dependent manner . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The retinoblastoma ( Rb ) tumor suppressor protein can regulate apoptosis triggered by DNA damage through an unknown mechanism . ^^^ This conclusion is consistent with the hypotheses that p84N5 functions in an Rb regulated cellular response that is similar to that triggered by DNA damage . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We show that caspase 3 is the primary executioner caspase in this system , necessary for cytochrome c / dATP inducible cleavage of fodrin , gelsolin , U 1 small nuclear ribonucleoprotein , DNA fragmentation factor 45 ( DFF 45 ) / inhibitor of caspase activated DNase ( ICAD ) , receptor interacting protein ( RIP ) , 10 linked inhibitor of apoptosis protein ( 10 IAP ) , signal transducer and activator of transcription 1 ( STAT 1 ) , topoisomerase 1 , vimentin , Rb , and lamin B but not for cleavage of poly ( ADP ribose ) polymerase ( PARP ) or lamin A . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Thus , RhoB attenuated alkylation induced increase in the DNA binding activity of NF kappaB and abrogated NF kappaB driven gene expression . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We examined loss of heterozygosity ( LOH ) in the p 53 , APC , DCC , RB , and NM 23 H1 gene regions by polymerase chain reaction ( PCR ) LOH assay using an automated fluorescent DNA sequencer employing four microsatellite markers ( p 53 , APC , DCC , NM 23 H1 ) . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We present a cytogenetic and fluorescence in situ hybridization ( FISH ) study , using centromeric probes for chromosomes 3 , 7 , 11 , and 18 , TP 53 gene ( 17p13 ) , and RB 1 locus ( 13q14 ) DNA probes , in four cases of plasma cell leukemia ( PCL ) . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Although E2F1 and p 53 mediate ectopic DNA synthesis and cell death in several tissues in Rb mutant embryos , both endoreduplication and apoptosis persisted in mgRb : Rb / : E2F1 / and mgRb : Rb / : p 53 / compound mutant muscles . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In the nucleus , c Abl activity is negatively regulated by the retinoblastoma protein ( RB ) and positively regulated by DNA damage signals . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Western blot analyses show that the expression of Rb , a key regulatory protein in G1 / S transition , and PCNA , integrally involved in mammalian cell DNA replication , were significantly reduced by treatment with YZ in PC 3 and DU 145 cells , respectively . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
These results are important for an understanding of the geometry of Rb translocations and suggest the study of DNA orientation as a new tool for investigating these rearrangements . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In contrast to the effect on DNA replication , the L13V substitution in large T antigen did not prevent complex formation with Hsc 70 and the Rb protein . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Transforming growth factor beta 1 induced growth inhibition of keratinocytes was characterized by decreases in DNA synthesis , accumulation of hypophosphorylated Rb , and the inhibition of the E2F : Rb responsive promoter , cdc 2 , and an induction of the p 21 promoter . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
PCR combined with nonisotopic heteroduplex SSCP analysis was used to screen leukocyte DNA for RB 1 germline mutations , exon by exon , without the use of restriction endonuclease digestion . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Furthermore , suppression of rb protein function by the HPV E 7 viral oncoprotein increased both spontaneous and DNA damage induced apoptosis . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
DNA sequence analysis with other vertebrate Rb sequences demonstrates that the medaka Rb cDNA is highly conserved in regions of functional importance . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In the attempt to elucidate whether this compound might affect genes playing a role in G1 / S phase transition , the expressions of p 53 , p 21 ( WAF 1 ) , cyclin D 1 and Rb , mainly involved in response to DNA damaging stress , were analyzed by Western blot . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In particular , it is now clear that the Rb / E2F pathway is critical in regulating the initiation of DNA replication . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
E2F 1 overexpression was sufficient to rescue Rb mediated reduction of telomerase activity , but an E2F 1 mutant defective in DNA and Rb binding activities failed to produce this effect . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We also show that in quiescent normal human cells this entire RBP 1 mSIN3 SAP 30 HDAC complex colocalizes with both RB family members and E2F4 in a limited number of discrete regions of the nucleus that in other studies have been shown to represent the initial origins of DNA replication following growth stimulation . ^^^ These results suggest that RB family members , at least in part , drive exit from the cell cycle by recruitment of this HDAC complex via RBP 1 to repress transcription from E2F dependent promoters and possibly to alter chromatin structure at DNA origins . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The amino acid sequence is homologous to LysR family regulatory proteins such as CatR of P . putida RB 1 and has helix turn helix DNA binding motif near its N terminal . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Taken together , these results provide novel links between components of the DNA replication machinery and the cell growth regulatory pathway involving the Rb tumor suppressor and E2F . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
MATERIAL AND METHODS : We determined the status of Rb tumor suppressor gene in oral squamous cell carcinoma using a DNA southern blotting assay with Digoxin ( Dig ) labeled Rb cDNA probe . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The differences in the spectrum of RB 1 mutations found in this and other European surveys are also discussed in the context of alternate DNA methylation and mismatch repair phenotypes . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The large subunit of replication factor C promotes cell survival after DNA damage in an LxCxE motif and Rb dependent manner . ^^^ Retinoblastoma ( Rb ) protein promotes cell survival after DNA damage . ^^^ We show here that the LxCxE binding site in Rb mediates both cell survival and cell cycle arrest after DNA damage . ^^^ RF C thus enhances cell survival after DNA damage in an Rb dependent manner . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Rubidium ( Rb ) influxes via Na / K pump and ouabain resistant pathways , and protein , RNA and DNA syntheses have been studied in human blood lymphocytes during the cell transit from quiescence to proliferation . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Stimulation of DNA polymerase alpha activity by cdk 2 phosphorylated Rb protein . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
RhoB is required to mediate apoptosis in neoplastically transformed cells after DNA damage . ^^^ In this study , we offer genetic evidence that the apoptotic response of neoplastically transformed cells to DNA damage requires RhoB , a member of the Rho family of actin cytoskeletal regulators . ^^^ This effect was specific insofar as rhoB deletion did not affect apoptotic susceptibility to agents that do not damage DNA . ^^^ We have demonstrated that RhoB alteration mediates the proapoptotic and antineoplastic effects of farnesyltransferase inhibitors , and we show here that RhoB alteration is also crucial for farnesyltransferase inhibitors to sensitize neoplastic cells to DNA damage induced cell death . ^^^ Our findings identify a pivotal role for RhoB in the apoptotic response of neoplastic cells to DNA damage at a novel regulatory point that may involve the actin cytoskeleton . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Applying the recently developed DNA array technique to a murine stroke model , we found that the gene coding for RhoB , a member of the family of GTPases that regulate a variety of signal transduction pathways , is upregulated in ischemia damaged neurons . ^^^ RhoB immunoreactivity precedes DNA single strand breaks and heralds the evolving infarct , making it an early predictor of neuronal death . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
To delineate the mechanism through which RB inhibits DNA replication , we generated cells which inducibly express a constitutively active allele of RB ( PSM RB ) . ^^^ However , activation of RB in S phase cells disrupts the chromatin tethering of PCNA , a requisite component of the DNA replication machinery . ^^^ The action of RB was S phase specific and did not inhibit the DNA damage mediated association of PCNA with chromatin . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We simultaneously used DNA probes for the following locus specific probes ( LSI ) ; LSI 13 ( Rb ) and D13S319 , which hybridize to 13q14 . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
DNA break binding motif similar to the functional part of BRCT related region of Rb . ^^^ It was shown that BRCTs of TopBP 1 , BRCA 1 , and BRCT Ws of Rb bound DNA ends and breaks . ^^^ We indicate here that the C terminus of p 53 tumor suppressor contains a DNA binding motif ( residues 327 333 in human ) , whose features are similar to those of the part of BRCT W in Rb with DNA binding activity . ^^^ These results suggest the common presence of DNA binding motifs that can recognize DNA ends or damages in major tumor suppressors , Rb , BRCA 1 and p 53 . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Rb represses E2F mainly by recruiting chromatin remodeling factors ( histone deacetylases and SWI / SNF complexes ) , the DNA methyltransferase DNMT 1 , and a histone methyltransferase . ^^^ A model in which Rb irreversibly represses E2F regulated genes in differentiated cells by an epigenetic mechanism linked to heterochromatin , and involving histone H 3 and promoter DNA methylation , is discussed . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
CONCLUSION : Episomal and integrated forms of HPV 16 DNA were found in VCs of the foot , along with alterations of c Ki ras , p 53 , and Rb genes . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The overall number of drug sorption centers for both drugs decreased in the presence of Pluronic implying the effect of Pluronic on the DNA conformation , which was confirmed by the electron paramagnetic resonance ( EPR ) experiments using Rb as a spin probe . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Inorganic , monovalent cations ( Li , Na , K , Rb , Cs ) , when present in the Debye Hckel layer of DNA , are found to bind to the negatively charged groups of the helix solely on the basis of their charge / mass ratio . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Previous work has established a role for p 53 in triggering apoptosis in response to DNA damage ; p 53 also induces apoptosis in response to deregulation of the Rb cell cycle pathway . ^^^ We conclude that the cellular response to DNA damage makes use of signals from the Rb / E2F cell cycle pathway . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Promotor hypermethylations were detected in O 6 methylguanine DNA methyltransferase ( MGMT ) , RB 1 , estrogen receptor , p 73 , p16INK4a , death associated protein kinase , p15INK4b , and p14ARF at 60 % , 34 % , 30 % , 16 % , 12 % , 10 % , 7 % , and 2 % , respectively . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
C cell hyperplasia foci were microdissected for DNA extraction to analyze the methylation pattern of androgen receptor alleles and microsatellite regions ( TP 53 , RB 1 , WT 1 , and NF 1 ) . ^^^ C cell hyperplasia foci showed heterogeneous DNA deletions revealed by loss of heterozygosity of TP 53 ( 12 of 20 ) , RB 1 ( 6 of 14 ) , and WT 1 ( 4 of 20 ) and hypodiploid G0 / G1 cells ( 14 of 20 ) , low cellular turnover ( MIB 1 index 4 . 5 % , in situ end labeling index 0 . 03 % ) , and significantly high nuclear area to DNA index ratio . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
To elucidate RB 1 germline mutations in Korean retinoblastoma patients , DNA samples from 14 children with bilateral ( including three familial cases ) and 19 children with unilateral retinoblastoma were analyzed . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Experiments with the reconstructed signal transduction cascade including DNA fragments demonstrated a substantial increase of the amount of phosphorylated OmpR in the presence of K ( + ) and to a lower extent in the presence of Na ( + ) , Rb ( + ) , and NH 4 ( + ) . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Treatment of Rb defective tumor cells with HDAC inhibitors greatly reduced the DNA binding activity of Oct 1 , a repressor of inducible HLA DRA promoter activation . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Analysis of E1A mutants failing to bind p 300 ( also called CBP ) or Rb shows that they are almost identical to wild type E1A in their ability to initially overcome a G ( 1 ) arrest in cells after DNA damage , while an E1A mutant failing to bind p 21 is not . ^^^ However , over time , this mutant , which can still target Rb , is far more efficient in accumulating cells with a DNA content greater than 4N but is similar to wild type E1A and the other E1A mutants in releasing cells from a p 53 mediated G ( 2 ) block following chemotherapeutic treatment . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Inactivation of the retinoblastoma ( Rb ) protein caused by gene mutation , association with oncoproteins from small DNA viruses , mutational inactivation of p 16 ( Ink4a ) , or overexpression of cyclin D is a common feature of many human cancer cells and is causally associated with the aberrant proliferation control of cancer cells ; whereas normal cells maintain an integrated cell cycle machinery and are subject to cell cycle checkpoint control by cyclin dependent kinase ( CDK ) inhibitors ( CKIs ) . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Furthermore , we demonstrated that the complexes containing RB and C / EBP proteins directly interacted with C EBP binding site on DNA . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Inactivation of lin 35 Rb , the single Rb related gene in C . elegans , substantially reduced the DNA replication and cell division defects in cyd 1 and cdk 4 mutant animals . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Measurements of hydrogen peroxide induced premature senescence : senescence associated beta galactosidase and DNA synthesis index in human diploid fibroblasts with down regulated p 53 or Rb . ^^^ Expression of human papillomaviral E 6 or / and E 7 genes results in reduction of p 53 or / and Rb protein levels and increases in ED 50 for DNA synthesis inhibition or SA beta gal expression . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The antagonism between RB and c Abl / p73 may modulate the function of p 53 to direct the choice between growth arrest and apoptosis in DNA damaged cells . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Chromosomes were analyzed by G banding , DNA from leukocytes and tumors was studied by segregation of polymorphisms within RB gene ( RB 1 ) and the DNA from chorionic villus by sequencing . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The retinoblastoma tumor suppressor ( RB ) and mismatch repair complexes are known to contribute to the appropriate cellular response to specific types of DNA damage . ^^^ RB deficient murine embryo fibroblasts continued a high degree of DNA replication following the induction of cisplatin damage , but were inhibited for G ( 2 ) / M progression . ^^^ In both Rb+ / + and Rb / cells , cyclin D 1 expression was attenuated following DNA damage . ^^^ As cyclin D 1 is a critical determinant of RB phosphorylation and cell cycle progression , we probed the pathway through which cyclin D 1 degradation occurs in response to DNA damage . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
RhoB expression was confirmed by sequencing an reverse transcriptase polymerase chain reaction generated DNA fragment of RhoB . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Our results demonstrate that activation of CCK ( B ) / gastrin receptor retrovirally transfected into Swiss 3T3 induces a potent synergistic effect on DNA synthesis , accumulation of cyclins D 1 , D 3 , and E and hyperphosphorylation of Rb in combination with insulin , EGF , or bombesin . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Growth arrest induced by DNA damage in mammalian cells requires the function of the retinoblastoma tumor suppressor protein ( RB ) . ^^^ RB deficient cells can not undergo G 1 , mid S or G 2 arrest following DNA damage , although they can activate the G 2 checkpoint , which is reversible . ^^^ RB deficient cells are also hypersensitive to DNA damage induced apoptosis . ^^^ Taken together , these results suggest that RB plays a critical role in determining the cell fate following DNA damage . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
METHODS : Single stranded conformation polymorphism ( SSCP ) analysis associated with direct DNA sequencing was used to identify point mutations in the coding sequence of Rb , p 53 , p 16 , p 15 and p 21 tumor suppressor genes , and multiplex PCR was used to detect big deletion in p 16 and p 15 genes . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
METHODS : Twenty two cases of paraffin embedded ICC tissue sections were microdissected and genotyped by PCR based DNA sequencing to detect the frequencies of loss of heterozygosity ( LOH ) of tumor suppressor genes APC , MCC , DCC , OGG 1 , p 53 , and RB 1 and of point mutation of Ki ras 2 oncogene . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We have created a potent transcriptional repressor of E2F dependent transcription by fusing the C terminal fragment of Rb ( p 56 ) to the DNA and DP 1 binding domains of E2F . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Antiproliferative effects of PPARgamma ligands are mediated by targeting critical cell cycle regulators , including Rb and p 27 ( Kip 1 ) , that regulate the progression of cells from G 1 phase into S phase to conduct DNA synthesis . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Using DNA affinity precipitation assays ( DAPA ) , we show that this complex contains SP 1 , NF 1 , Rb and HDAC 1 proteins . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
They furthermore argue that despite the presence of KSHV DNA and expression of a latent gene program Rb function is intact in PEL . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Hybridizations with locus specific DNA probes demonstrated a high incidence of deletion for the tumour suppressor genes p 53 and retinoblastoma ( Rb ) , and for the oncogene cyclin D 1 , comparable in all carcinoma types . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We also studied Rb reconstituted cell lines postirradiation to gain insight into the role of Rb following DNA damage . ^^^ Additionally , Rb protein protected cells from DNA damage and may also play a role in radiation induced G 2 delay . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
However , RGS12TS S inhibited S phase DNA synthesis in various tumor cell lines independently of Rb and p 53 proteins , and its prolonged expression promoted formation of multinucleated cells . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Rb protein translocated into nucleoli after DNA replication completed , and the nucleolar Rb was shown to be in the hyperphosphorylated form by immunoblotting . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
RB , MDM 2 ) , DNA damage recognition and repair ( e . g . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
However , unlike the small DNA tumor virus proteins , individual HCMV IE proteins target different RB family members . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In the central nervous system , loss of E2F1 or E2F3 can almost completely eliminate the ectopic DNA replication and apoptosis observed in Rb ( / ) embryos , and loss of E2F2 partially reduces the unscheduled DNA replication and has no effect on apoptosis . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The Rb tumor suppressor protein can inhibit apoptosis triggered by DNA damage , but how it does so is unclear . ^^^ Further , p84N5 is a potential mediator of Rb protein ' s effects on DNA damage induced apoptosis . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Without rumour material , the germline status of potentially sporadic cases will remain undetermined since the absence of detectable RB 1 coding region mutations does not exclude all possible mutations in the RB 1 gene , which is too large for DNA analysis . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Inactivation by DNA methylation of several genes of the RB 1 pathway is common to cirrhosis and HCC . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Thirteen cases had lost one RB 1 copy relative to centromere 17 copy number and total DNA content . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Based on these results , we suggest that : ( 1 ) the wild type p 53 function but not p 21 ( waf1 / cip1 ) expression is necessary for GGR of chemical induced bulky DNA adducts ; ( 2 ) the Rb gene product does not play a significant role in NER ; and ( 3 ) the modulation of NER by p 53 may be independent of its function in the regulation of cell cycle arrest upon chemically induced DNA damage . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Twenty one probands , twelve with bilateral and nine with unilateral retinoblastoma , were screened for mutations in the RB 1 gene using genomic DNA from peripheral blood leukocytes as well as tumors . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Mechanism of action studies have confirmed that the phosphorylation of CDK 2 substrates such as RB , histone H 1 , and DNA polymerase alpha ( p 70 subunit ) is reduced in the presence of compound 14 . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Inhibitor of differentiation or DNA binding ( Id 1 ) , a helix loop helix transcription factor , has recently been shown to inactivate the retinoblastoma ( RB ) / p16 ( INK4a ) pathway through down regulation of p 16 ( INK4a ) and increasing phosphorylation of RB in certain cell types . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The status of RB 1 , INK4A , INK4B , INK4C , INK4D , and ARF in 13 adult and 2 juvenile ovarian GCTs was determined by reverse transcription polymerase chain reaction of total RNA and exon specific sequencing of genomic DNA . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Although ectopic cyclins partially overcome RB mediated S phase arrest at the replication fork , DNA replication remains inhibited and cells fail to progress to G ( 2 ) phase . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Bcl 10 ( L ) mice display a similar neurodevelopmental phenotype as rb , DNA ligase 4 , and XRCC 4 mutant embryos , suggesting that endogenous Bcl 10 ( L ) expression may protect immature neurons from death caused by DNA damage and / or cell cycle dysregulation . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
However , E 7 C terminal mutants that were able to inactivate Rb were unable to bypass DNA damage induced G ( 1 ) arrest and keratinocyte senescence , suggesting that the E 7 C terminus may target additional G ( 1 ) regulators . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Fibroblasts expressing Rb MI protein are protected from apoptosis induced by the tumour necrosis factor alpha type 1 receptor ( TNFRI ) but remain sensitive to cell death induced by DNA damage . ^^^ Correspondingly , the release of cytochrome c and the activation of caspase 3 induced by TNFRI , but not by DNA damage , are defective in cells expressing Rb MI . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Limited deletions of plant DNA have been observed at the site of insertion of T DNA as well as in its left ( LB ) and right ( RB ) T DNA signal sequences . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The genetic components of the RB : CDK : cyclin : p 16 tumor suppressor pathway undergo mutational and epigenetic alterations in a wide range of human cancers and serve as critical targets for inactivation by the transforming oncoproteins of several DNA tumor viruses . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The characterizations of the mutation in genomic DNA , RNA and protein have provided new evidences which enhance credence to the idea that low penetrance retinoblastoma is caused by only partially functional disable of Rb 1 . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Rb is also implicated in the regulation of replication machinery and in the control of cell cycle checkpoints in response to DNA damage , further supporting such a role for Rb . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Activation of the retinoblastoma ( RB ) protein through dephosphorylation arises in cells upon exit from M phase and in response to environmental stresses , including DNA damage . ^^^ Importantly these cells also do not respond with RB activation after DNA damage during S phase . ^^^ Moreover and as a consequence they display phenotypes classically associated with RB ( ) cells , showing accelerated apoptosis after DNA damage and DNA re replication after spindle checkpoint activation . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Thus , regulation of the RB function may be an important aspect in the maintenance of cell cycle checkpoints in DNA damage response . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We found that expression of PSM RB significantly attenuates > 200 targets , the majority of which are involved in cell cycle control ( DNA replication or G 2 M ) , DNA repair , or transcription / chromatin structure . ^^^ Thus , these results have utility in assigning RB pathway activation in more complex systems of cell cycle inhibition ( e . g . , mitogen withdrawal , senescence , or DNA damage checkpoint ) . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
DNA tumor virus oncoproteins bind and inactivate Rb by interfering with the Rb / HDAC1 interaction . ^^^ These findings indicate the existence of a pathway of Rb regulation supporting Che 1 as the cellular counterpart of DNA tumor virus oncoproteins . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Prohibitin , like Rb , could repress transcription from SV 40 and major late promoters when recruited directly to DNA . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The remaining genes are part of either the p 53 p21 and / or p 16 Rb pathways , which induce cell cycle arrest or apoptosis in response to DNA damage . ^^^ CONCLUSIONS These findings suggest that the cellular mechanisms that sense and respond to DNA damage , namely the p 53 p21 pathway and the p 16 Rb pathway , are activated . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The preadipocytes traverse the G ( 1 ) S checkpoint synchronously as evidenced by the expressionactivation of cdk 2 cyclin EA , turnover of p27kip1 , hyperphosphorylation of Rb , translocation of cyclin D ( 1 ) from nuclei to cytoplasm and GSK 3beta from cytoplasm to nuclei , and incorporation of [ ( 3 ) H ] thymidine into DNA . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Furthermore , although cellular DNA replication was blocked , elevation of cyclin E / A expression and accumulation of hyperphosphorylated forms of Rb protein were observed , a post G ( 1 ) / S phase characteristic of cells . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
This E2F activity is independent of Rb binding , but contingent on binding DNA . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The genes associated with bladder carcinogenesis include oncogenes ( such as H ras , FGFR 3 , erbB 2 , CCND 1 , mdm 2 ) , tumor suppressor genes ( such as INK4A / ARF , Rb , TP 53 , PTEN , TSC 1 , PTCH , DBCCR 1 ) , and DNA mismatch repair genes , etc . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
METHODS : 1 ) Southern blot hybridization was used to analyse the tumor cell DNA of 327th passage of SO Rb 50 cell line . 2 ) A promoter and 27 exons of Rb gene were screened exon by exon by using PCR SSCP at 415th and 713th passages of SO Rb 50 cell line . 3 ) Three cell cloning strains named as MC 2 , MC 3 and MC 4 were isolated by single cell cloning technique from the SO Rb 50 775 , and mutation of Rb gene were also screened exon by exon by using PCR SSCP HA in MC 2 11 , MC 3 11 , MC 4 11 and MC 3 138 . ^^^ RESULTS : The 3 . 5 Kb , 2 . 9 Kb and 1 . 0 Kb bands were deleted in the DNA of SO Rb 50 327 tumor cells , showing the deletion of Rb gene in SO Rb 50 cell line . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Some common genetic processes ( e . g . telomere dysfunction , changes in p 53 and Rb activity and in DNA repair , accumulation of DNA lesions , genomic instability ) play a critical role both in carcinogenesis and aging . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
L 1 ) were employed into HXO RB 44 cell line respectively for 24 hours , then apoptotic effects on it were decided by observing the DNA ladders on agarose gel electrophoresis . ^^^ After that , one chemotherapeutic agent with the most evident DNA ladders was applied into HXO RB 44 cell line for 4 , 8 , 16 , 24 , 48 and 72 hours respectively , and the DNA ladders on agarose gel were also surveyed . ^^^ RESULTS : Typical DNA ladders were shown on agarose gel after HXO RB 44 cell line were exposed to 10 ( 6 ) 10 ( 5 ) mol . ^^^ No DNA ladders did emerge when the RB cells were treated by the rest ten chemotherapeutic agents . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Mechanisms by which DNA tumor virus oncoproteins target the Rb family of pocket proteins . ^^^ This review compares and contrasts the approaches DNA tumor viruses have evolved to antagonize Rb family members ranging from relatively simple equilibrium dissociation of pRb from cellular pRb binding factors to chaperone mediated alterations in pocket protein stability and phosphorylation levels . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Upon dissociation from Rb , the glycosylated YY 1 is free to bind DNA . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In addition , telomerase negative cells , which maintain their telomere length through an alternative lengthening of telomeres DNA recombination pathway , showed no cytotoxicity to RB 94 . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
It interacts directly or indirectly with a variety of important proteins , including oncogene proteins ( c myc , E2F ) , tumor suppressor proteins ( p 53 , RB , BRCA 2 ) , DNA damage repair proteins ( RAD 50 , RAD 51 ) , cell cycle regulators ( cyclin , CDK ) , transcriptional regulators ( RNA polymerase 2 ) and others related to the important biological events . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Retinoblastoma gene product ( Rb ) , a negative regulator of DNA methylation , bound to the allosteric site of hDNMT 1 and inhibited methylation , suggesting Rb may regulate methylation spreading . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Like in Rb knockout embryos , rescued animals showed a marked increase in DNA replication and cell division in the CNS . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
These changes were accompanied by increased expression of S and G ( 2 ) phase cyclins and cyclin dependent kinases ( cdk ) , increased Rb phosphorylation , and increased E2F 1 DNA binding activity . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
These observations suggest that the interaction of YY 1 with hypophosphorylated Rb in resting B cells prevents interaction of YY 1 with DNA . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Therefore , we examined the involvement of p 38 MAPK signaling in AngII stimulated DNA synthesis , phosphorylation of the retinoblastoma protein ( Rb ) , and expression of the G 1 phase cyclin D 1 in human coronary artery smooth muscle cells ( CASMC ) . ^^^ AngII induced phosphorylation of Rb ( Ser 795 and Ser 807 / 811 ) , cyclin D 1 expression , and DNA synthesis was also markedly enhanced by pharmacological inhibition of the p 38 MAPK pathway . ^^^ The present study demonstrates that p 38 MAPK negatively regulates AngII induced ERK1 / 2 activity , Rb phosphorylation , cyclin D 1 expression , and DNA synthesis in human CASMC . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Despite this oncogenic lesion , Y 1 cells retain tight regulatory mechanisms of cell cycle control typified by the sequential events comprising the mitogenic response triggered by FGF 2 in G0 / G1 arrested Y 1 cells : 1 ) activation of ERK1 / 2 and PI3K , by 5 minutes ; 2 ) induction of c Fos and c Myc proteins by 2 hours ; 3 ) induction of cyclin D 1 protein by 5 hours ; 4 ) phosphorylation of Rb protein between 6 and 8 hours ; 5 ) onset of DNA synthesis by 8 9 hours . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Prognostic importance of the nuclear proteins p 53 and Rb in conjunction with DNA , nuclear morphometry and grading in endometrial carcinoma . ^^^ In a series of 227 cases of endometrial carcinomas in FIGO stages 1 4 , treated during the years 1984 89 , immunohistochemical staining for the protein products of the two tumor suppressor genes p 53 and retinoblastoma ( Rb ) were evaluated as prognostic factors with regard to tumor stage , FIGO grade , nuclear grade , morphometric nuclear parameters , DNA ploidy and S phase fraction . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Transcription of mouse DNA methyltransferase 1 ( Dnmt 1 ) is regulated by both E2F Rb HDAC dependent and independent pathways . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
After genomic DNA extraction , an allelotyping analysis targeting microsatellites linked to Rb and p 53 genes , and 9p21 , 7q31 and 5q21 regions was performed . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The frequencies of aberrant methylation were : 64 % for thrombospondin 1 ( THBS 1 ) ; 30 % for tissue inhibitor of metalloproteinase 3 ( TIMP 3 ) ; 27 % for O 6 methylguanine DNA methyltransferase ( MGMT ) ; 25 % for p 73 ; 18 % for RB 1 ; 14 % for death associated protein kinase ( DAPK ) , p14ARF , p16INK4a and caspase 8 , and 0 % for TP 53 and glutathione S transferase P 1 ( GSTP 1 ) . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
To study the interactions between monovalent cations and DNA , the heavier alkali metal ion Rb ( + ) was used to replace Na ( + ) and K ( + ) in the Z DNA crystal and the structure was analyzed using 10 ray cryocrystallography . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We have used DNA microarray analysis to identify gene expression profiles in wild type and Rb null mouse embryo fibroblasts , as well as cells lacking other Rb family members , as an approach to developing a more complete understanding of Rb function . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In this investigation , we have determined the effect of the DNA demethylating drug decitabine ( DAC ; 5 aza 2 ' deoxycytidine ) on the growth , cell cycle kinetics , RB phosphorylation , and expression of p 16 ( INK4a ) and p 21 ( WAF 1 ) in EBV human myeloma and EBV+ lymphoblastic cell lines possessing silenced , methylated p 16 ( INK4a ) genes to : ( 1 ) . evaluate its potential as a therapeutic agent and ( 2 ) . investigate its mechanism of action . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Expression of p 16 , Rb , and p 53 proteins in squamous cell carcinomas of the anorectal region harboring human papillomavirus DNA . ^^^ These observations indicate that overexpression of p 16 and loss of Rb nuclear staining are commonly associated with high risk HPV infection , which may serve as useful surrogate biomarkers for identifying squamous cell carcinomas harboring HPV DNA . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Importantly , the region of acquired DNA loss includes two established tumour suppressor genes , the retinoblastoma gene , RB ( RB 1 ) and BRCA 2 . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Integration of viral DNA into host cell DNA is essential for this cancer development , promoting disruption of the HPV E 2 gene , thus leading to unregulated increases in E 6 and E 7 proteins and inactivating the products of p 53 and Rb tumor suppressor genes . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
BACKGROUND : Several tumor suppressor genes such as p16INK4 , TP 53 , RB 1 y p21WAF1 are involved in cell cycle regulation in response to DNA damage and belong to the complex pathway that regulates cell proliferation and / or differentiation . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
On the other hand , in cultures of rMFs , cell number increases along with DNA synthesis , and these cells do not become multinuclear . `` Activated ' ' HSCs produce higher levels of cyclin D ( 1 ) and E ( 1 ) transcripts than rMFs , which correlates with their increased levels of phosphorylated retinoblastoma ( Rb ) protein . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
PURPOSE : To detect DNA and proteins of human papillomavirus ( HPV ) in paraffin embedded retinoblastoma ( RB ) tissue , to identify the viral types present and to describe a possible association between the presence of HPV and a particular form of RB . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
All 27 exons , intron exon boundaries , and essential promoter region of RB 1 gene were then sequenced in genomic DNA from 4 pituitary adenomas with allelic imbalance on 13q14 including one adenoma without pRb expression and 3 adenomas with pRb expression . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The N terminal DNA J domain of T Ag cooperates with the LXCXE motif to override RB mediated repression of E2F dependent transcription . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In the second type , which was more frequent in RB , the link was direct , without any overlaps or filler DNA . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Additionally , fusion of the 3 ' RhoB untranslated region ( UTR ) to a constitutively expressed reporter gene caused serum and growth factor as well as DNA damage inducible expression . ^^^ These observations are consistent with the view that RhoB mRNA is produced constitutively but its abundance is controlled in response to growth factors , and other signals including DNA damage , by stabilization through elements within the 3 ' UTR . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We investigated the involvement of the RB 1 gene in a case of refractory anemia with del ( 13 ) ( q12q14 ) by florescence in situ hybridization ( FISH ) analysis using the RB 1 locus ( 13q14 ) DNA probe . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Mutation analyses were performed on DNA samples from these patients to test for heritable RB 1 mutations . ^^^ METHODS : A genomic DNA analysis of RB 1 gene . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The Rb protein suppresses development of an abnormal state of endoreduplication arising after S phase DNA damage . ^^^ Like S phase DNA damage in Rb ( / ) cells , specific interruption of PP2A function in irradiated , S phase wt cells also elicited a state of endoreduplication . ^^^ Thus , PP2A normally licenses the recruitment of Rb to chromatin sites in S phase from which , after DNA damage , it relocalizes to selected replication control sites and suppresses abnormal , postdamage rereplicative activity . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Contrarily , estradiol inhibits serum dependent retinoblastoma protein ( Rb ) phosphorylation and blocks DNA synthesis . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Clearly , any damage to the cell cycle or to DNA integrity is a potent trigger of apoptosis and Rb involvement . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
EXPERIMENTAL DESIGN : We examined the DNA methylation status of 12 tumor related genes ( NF 2 , RB 1 , p 14 ( ARF ) , p 16 ( INK4a ) , p 73 , TIMP 3 , MGMT , DAPK , THBS 1 , caspase 8 , TP 53 , and GSTP 1 ) in 44 sporadic and / or NF 2 associated schwannomas using methylation specific PCR . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Microsatellite polymorphisms at the Rb 1 and BRCA 2 loci were polymerase chain reaction ( PCR ) amplified from each patient ' s paired tumour and leucocyte DNA samples , using oligonucleotide primers flanking the repeat sequence . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Accompanied by the endonucleosomal fragmentation of DNA , the newly formed p115 / hypo / RB is immediately cleaved by a protease that has properties of the interleukin 1beta converting enzyme family . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
RB signaling prevents replication dependent DNA double strand breaks following genotoxic insult . ^^^ The retinoblastoma tumor suppressor ( RB ) is crucial for the maintenance of the DNA damage checkpoint function because it elicits cell cycle arrest in response to a variety of genotoxic stresses . ^^^ Although sporadic loss of RB is characteristic of most cancers and results in the bypass of the DNA damage checkpoint , the consequence of RB loss upon chemotherapeutic responsiveness has been largely uninvestigated . ^^^ This system enabled us to examine the DNA damage response of adult cells following acute RB deletion . ^^^ Using this system , we demonstrated that loss of RB disrupted the DNA damage checkpoint elicited by either cisplatin or camptothecin exposure . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
When nuclear export of the actin was induced by 12 O tetradecanoylphorbol 13 acetate , DNA synthesis of the senescent cells increased significantly , accompanied with changes of morphologic and biochemical profiles , such as increased RB protein phosphorylation and decreased expressions of p 21 ( WAF 1 ) , cytoplasmic p extracellular signal regulated kinase 1 / 2 , and caveolins 1 and 2 . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
One intriguing speculation might be that the down regulation of RhoB in osteoarthritic chondrocytes is at least partly a prerequisite for the sustained pre or para apoptotic phenotype of osteoarthritic chondrocytes , because RhoB is known to be one important molecule in the induction of apoptotic cell death in response to DNA damage and osteoarthritic chondrocytes are known to have significant DNA damage . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We have previously characterized a medaka homologue of the human Rb complementary DNA that is conserved in regions of functional importance . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Rb , as well as Cdc 6 , NPAT , and nucleophosmin , critical components of cell proliferation and DNA replication , respectively , are targets of Cyclin E / Cdk2 phosphorylation . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Here we define a transcription mediated pathway in which deregulation of E2F1 by ectopic E2F expression or Rb inactivation by E 7 of human papillomavirus type 16 signals apoptosis by inducing the expression of Chk 2 , a component of the DNA damage response . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Furthermore , inhibitions of Rb phosphorylation and DNA synthesis by EPE were not suppressed with the addition of catalase . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
When challenged with both DNA damaging and antimetabolite chemotherapeutics , RB was required for primary adult cells to undergo DNA damage checkpoint responses and loss of RB resulted in enhanced aneuploidy following challenge . ^^^ These studies underscore the critical role of RB in DNA damage checkpoint signaling and demonstrate that RB mediates chemotherapeutic induced cell cycle inhibition in adult fibroblasts by distinct mechanisms . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In a search for proteins that can improve the survival of Chk 1 deficient cells exposed to DNA damage , we identified fission yeast Msc 1 , which is homologous to a mammalian protein that binds to the tumor suppressor Rb ( RBP 2 ) . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
To search for the possible mechanisms of gene silencing , loss of heterozygosity ( LOH ) was analysed followed by testing DNA methylation and protein expression for those genes found with the highest frequency of LOH ( CDKN2A ( 55 . 4 % ) , MLH 1 ( 46 . 0 % ) , RB 1 ( 35 . 7 % ) ) . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Together , these data link the expression of geminin to the RB / E2F pathway and represent the first promoter analysis of this important regulator of DNA replication . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Two independent RB 1 inactivating mutations in peripheral blood DNA of a hereditary retinoblastoma patient . ^^^ We report the presence of a hemizygous inactivating germ line RB 1 mutation ( a recurrent g . 78250C > T transition , resulting in a stop codon in exon 17 ) in peripheral blood DNA from a patient with hereditary bilateral retinoblastoma . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Competitive Na ( + ) and Rb ( + ) binding in the minor groove of DNA . ^^^ Here , we present new ( 23 ) Na and ( 87 ) Rb magnetic relaxation dispersion ( MRD ) data that demonstrate competitive and long lived binding of Na ( + ) and Rb ( + ) ions in the minor groove of the B DNA duplex [ d ( CGCGAATTCGCG ) ] ( 2 ) . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
RB reversibly inhibits DNA replication via two temporally distinct mechanisms . ^^^ However , recent data have suggested that the direct interaction of RB with replication factors or sites of DNA synthesis may contribute to its ability to inhibit S phase . ^^^ Here we show that RB does not exert a cis acting effect on DNA replication . ^^^ Following long term activation of RB , we observed the attenuation of multiple replication factors , the complete cessation of DNA synthesis , and impaired replicative capacity in vitro . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
When mammalian cells contain damaged DNA , the p 53 tumor suppressor and the Rb family of transcriptional repressors work together to downregulate a large number of genes that encode proteins required for G 2 and M . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
These findings suggest that there may be a more intimate relationship between the apoptosis pathways resulting from loss of proper Rb mediated growth control and apoptosis resulting from the accumulation of DNA damage . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Parameters of oxidative DNA damage and repair , lipid peroxidation , and superoxide dismutase ( SOD ) activity were measured across six mouse brain regions 24 h after administration of a single dose of RB . ^^^ Lipid peroxidation and oxidative DNA damage were either unchanged or decreased in all brain regions in RB treated mice compared with vehicle treated mice . ^^^ In conclusion , specific brain regions responded to an acute dose of RB by significantly altering SOD and OGG 1 activities to maintain the degree of oxidative DNA damage equal to , or less than , that of normal steady state levels . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Lmna ( / ) cells resemble Rb ( / ) cells , exhibiting altered cell cycle properties and reduced capacity to undergo cell cycle arrest in response to DNA damage . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Analysis of RB action in DNA damage checkpoint response . ^^^ The retinoblastoma tumor suppressor ( RB ) is a critical effector of DNA damage checkpoint function by eliciting G 1 phase cell cycle arrest following genotoxic stress . ^^^ Here , we describe methodologies for evaluation of three facets of RB action in the DNA damage checkpoint response : ( 1 ) transcriptional repression of E2F regulated genes ( cyclin A reporter assay ) ; ( 2 ) induction of cell cycle arrest ( Brd U incorporation assay ) ; and ( 3 ) inhibition of DNA double strand break accumulation ( phosphorylated histone H2A . ^^^ Together , this combination of techniques allows the evaluation of RB action in the coordinated checkpoint response to DNA damage . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Detection of human papillomavirus DNA and expression of p 16 , Rb , and p 53 proteins in small cell carcinomas of the uterine cervix . ^^^ In this study , 22 cases of primary small cell carcinoma of the uterine cervix were subjected to broad spectrum HPV DNA amplification and typing , and immunohistochemically examined for the expression of p 16 , Rb , and p 53 proteins . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Rb 1 knockdown affected G1 / S and G2 / M transitions of the cell cycle , DNA replication and repair , mitosis , and apoptosis , indicating that siRNA mediated transient elimination of Rb 1 mimics the control of cell cycle through Rb 1 dissociation from E2F . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In quiescent , estrogen deprived MCF 7 cells , estradiol did not induce a rapid activation of either the MAPK / ERK or phosphatidylinositol 3 kinase ( PI 3K ) / Akt pathway , whereas the entry into the cell cycle was documented by the successive inductions of cyclin D 1 expression , hyperphosphorylation of the retinoblastoma protein ( Rb ) , activity of the promoter of the cyclin A gene , and DNA synthesis . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The effects of RB as a sensitizing agent on DNA in white cells , bacteria and viruses in combination with UV light exposure have been evaluated . ^^^ UV mediated DNA degradation in Jurkat T cells and leukocytes in plasma as measured by the FlowTACS assay was significantly increased in the presence of RB . ^^^ Agarose gel electrophoretic analysis of DNA in Escherichia coli and leukocytes in plasma demonstrated enhanced DNA degradation in the presence of RB . ^^^ UV mediated oxidative damage in calf thymus DNA was also enhanced in the presence of RB . ^^^ These observations clearly demonstrate that the presence of RB and UV light selectively enhances damage to the guanine bases in DNA . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Like the DNA tumor virus proteins , the human cytomegalovirus ( HCMV ) pp 71 protein contains a retinoblastoma protein ( Rb ) binding motif ( LxCxD ) , and stimulates DNA synthesis in quiescent cells . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Accordingly , we examined the DNA methylation status of ten tumor related genes ( RB 1 , p 16 ( INK4a ) , p 73 , MGMT , ER , DAPK , TIMP 3 , p 14 ( ARF ) , THBS 1 , and Caspase 8 ) in 98 meningiomas ( 68 grade 1 ; 27 grade 2 ; and 3 grade 3 samples ) using methylation specific PCR and sequencing . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
An immediate response in stressed cells was dephosphorylation of retinoblastoma ( Rb ) and cessation of DNA synthesis . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Amplification of genomic DNA by the polymerase chain reaction ( PCR ) showed no E2F1 gene amplification or mutation in the Rb binding region of E2F1 in MDS patients , nor was transcriptional up regulation noted when E2F1 messenger RNA ( mRNA ) levels were estimated with real time reverse transcriptase PCR . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Three different types of molecular markers [ allozymes , mitochondrial DNA ( mtDNA ) and microsatellites ] consistently indicated partial reproductive isolation between the RB and the SU ecotypes at a particular site . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Acute Rb excision from conditional knockout myotubes caused reexpression of E2F transcriptional activity , cyclin E and A kinase activities , PCNA , DNA ligase 1 , RPA , and MCM 2 , but did not induce DNA synthesis , showing that pRb is not indispensable to preserve the postmitotic state of these cells . ^^^ Finally , Rb removal induced no DNA synthesis even in pocket protein null cells . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Many tumor suppressor genes ( such as p 16 , Rb , VHL , E cadherin , and hMLH 1 ) that are silenced by mutation are also inactivated by gene silencing through DNA methylation . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
These include the telomerase pathway required to maintain telomere ends , the p 53 and Rb pathways required to direct senescence in response to DNA damage , telomere shortening and mitogenic signals , and the insulin like growth factor Akt pathway that may regulate lifespan and cell proliferation . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We show that the senescence inducing actions of Smurf 2 occur in the absence of detectable DNA damage or stress response , that Smurf 2 ' s effects require a novel function distinct from its E 3 activity , that Smurf 2 recruits the Rb and p 53 pathways for senescence induction , and that while p 21 is elevated by Smurf 2 , Smurf 2 mediated senescence is independent of p 21 . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The exact mechanisms of immortalization are not clear , although inactivation of the RB pathway , and / or the p 53 pathway and activation of telomerase has been shown to be necessary for immortalization of certain cell types with DNA viruses or hTERT . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The positive factors up regulated by AtE2Fa emcompassed genes for G1 / S transition , DNA synthesis and mitosis , and the negative factors up regulated by AtE2Fa included RB 1 , encoding the E2F binding protein , as well as KRP 3 and KRP 5 , encoding the plant CDK inhibitors . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Protein DNA interactions at the retinoblastoma ( RB 1 ) promoter , including the ' essential regulatory region ' , were investigated using novel DNA targeted nitrogen mustards in intact human cells . ^^^ Our results demonstrate that , in intact cells , footprints were observed at the E2F , ATF and RBF1 / Sp1 DNA binding motifs in the RB 1 promoter . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
METHODS : The human Rb cell lines Y 79 and WERI Rb 1 were evaluated for their response to the Fas cross linking antibody CH 11 and recombinant TRAIL , as well as for cell surface presence and mutational status of Fas , DR 4 , and DR 5 by flow cytometry and genomic DNA sequencing , respectively . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Peptide conjugates of the xanthene dye rose bengal ( RB ) are described featuring sequences that promote DNA binding . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
METHODS : Twelve day 3 embryos , obtained by IVF with intracytoplasmic sperm injection , underwent single cell DNA testing via polymerase chain reaction and restriction enzyme analysis to detect the presence of a paternal RB 1 mutation . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
AMP mediated inhibition of DNA replication and S phase progression : involvement of Rb , p21Cip1 , and PCNA . cAMP exerts an antiproliferative effect on a number of cell types including lymphocytes . ^^^ The cAMP induced inhibition of DNA synthesis was associated with the increased binding of p21Cip1 to Cdk 2 cyclin complexes , inhibition of Cdk 2 kinase activity , dephosphorylation of Rb , and dissociation of PCNA from chromatin in S phase cells . ^^^ The ability of cAMP to inhibit DNA replication and trigger release of PCNA from chromatin required Rb and p21Cip1 proteins , since both processes were only marginally affected by increased levels of cAMP in Rb / and p21Cip1 / 3T3 fibroblasts . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Recent studies with DNA viruses have contributed to our understanding of critical tumor targets ( such as EGFR , PP2A , Rb and p 53 ) and have an impact on the development of novel therapies , including oncolytic viral agents , for the treatment of cancer . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In coimmunoprecipitations and ChIP experiments , Rb was found to associate with STAT 3 on DNA and we provide evidence that Rb binds directly to the transcription factor . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In the present study , we show that enforced telomerase activity induces the bypass of replicative senescence in limbal and conjunctival keratinocytes , without the inactivation of the p 16 ( INK4a ) / Rb pathway or the abrogation of p 53 expression . hTERT transduced limbal and conjunctival keratinocytes are capable to respond to both growth inhibitory and differentiation stimuli , since they undergo growth arrest in response to phorbol esters , and activate p 53 upon DNA damage . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Thus , Rb inactivation is a pre requisite for Abl activation by DNA damage or TNF . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
However , the addition of polyamines did not reverse EPE induced decrease in DNA synthesis and phosphorylation of Rb protein , and EPE induced translocation of AIF . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Consistent with their effect on Rb phosphorylation , PPARgamma ligands inhibit the mitogenic induction of minichromosome maintenance ( MCM ) proteins 6 and 7 , two E2F regulated S phase genes essential for DNA replication . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
METHODS : Synovial tissue from 20 patients with active proliferative RA and 28 patients with RA in remission was immunohistochemically examined for expression of p 53 , p 63 , p 21 , p 27 , p 16 , cyclin D 1 , CDK 4 , RB , E2F , Ki 67 on tissue microarrays and by DNA flow cytometry for cell cycle phases . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Mouse embryonic fibroblasts that are triply deficient for RB 1 ( retinoblastoma 1 ) , RBL 1 ( retinoblastoma like 1 ) and RBL 2 ( retinoblastoma like 2 ) known as TKO cells show a marked genomic instability , which is coincidental with decreased DNA methylation , increased acetylation of histone H 3 and decreased tri methylation of histone H 4 at lysine 20 ( H4K20 ) . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In 24 ( 13 % ) of these patients , testing of blood DNA showed a constitutional RB 1 mutation . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The retinoblastoma tumor suppressor ( RB ) is functionally inactivated in the majority of cancers and is a critical mediator of DNA damage checkpoints . ^^^ This model system enabled us to investigate the temporal role of RB loss on cell cycle checkpoints and DNA damage repair following ultraviolet ( UV ) and ionizing radiation ( IR ) damage . ^^^ RB loss had a differential effect upon repair of the major DNA lesions induced by IR and UV . ^^^ Whereas RB did not affect resolution of DNA double strand breaks , RB deficient cells exhibited accelerated repair of pyrimidine pyrimidone photoproducts ( 6 4 PP ) . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Of the 1 , 383 genes , 159 genes , including oncogenes ELK 1 , FRAT 2 , R RAS , RHOB , and RHO 6 , were further selected as gene candidates that are silenced by DNA methylation in normal stomach mucosa but are activated by DNA demethylation in a subset of gastric cancers . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Rb suppression also caused extra DNA replication in endoreduplicating leaf cells , suggesting a role of Rb in the endocycle . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
To identify the genes targeted by gains on chromosome 1q , we used quantitative multiplex PCR to determine DNA copy number changes in 76 primary tumors and 6 Rb cell lines . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The Ink4a / Arf ( CDKN2a ) locus encodes two proteins that regulate two of the most important tumor suppressor pathways represented by p 53 and Rb . ( 1 ) Loss of either p 16 ( INK4a ) or p 19 ( ARF ) was recently reported to reduce the ability of mouse cells to repair UV induced DNA damage and to induce a UV mutator phenotype . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
To test this hypothesis , we investigated the effects of FTIs on the generation of ROS , DNA double strand breaks ( DSB ) , DNA damage responses , and RhoB , and the effects of quenching ROS on these FTI effects . ^^^ RhoB , a mediator of the antineoplastic effect of FTIs and a protein inducible by DNA damage , was increased by FTIs . ^^^ We concluded that FTIs induced oxidative DNA damage by inducing ROS and initiated DNA damage responses , including RhoB induction , and there was a complex relationship among FTIs , farnesyltransferase , ROS , and RhoB . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Genetic response to DNA damage : proapoptotic targets of RhoB include modules for p 53 response and susceptibility to Alzheimer ' s disease . ^^^ To gain insight into this question , we compared the genetic response of cells with different rhoB genotypes to the DNA damaging anticancer drug doxorubicin ( DOX ) . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Southern blot analysis of DNA from these samples revealed loss of heterozygosity ( LOH ) at the RB 1 locus in 6 of 24 informative cases ( 25 % ) compared to normal blood DNAs from the same patients . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
DNA methylation status of five tumor suppressor genes , HIC 1 , p 16 , RASSF1A , p 53 , and RB 1 was detected in 85 . 2 , 48 . 1 , 44 . 4 , 22 . 2 , and 0 % of HCCs , respectively . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Functions attributed to pp 71 include the ability to enhance the infectivity of viral DNA , bind to and target hypophosphorylated Rb family member proteins for degradation , drive quiescent cells into the cell cycle , and bind to the cellular protein hDaxx . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Microdissected RB tissues and their matched blood DNAs were analyzed for PCR based LOH by using fluorescence based DNA sequencing technology . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
DNA chromatin immunoprecipitation experiment shows that ICBP 90 binds to the RB 1 gene promoter under its methylated status . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
During myotube hypertrophy , the increase in RNA content was accompanied by an increase in tumor suppressor protein retinoblastoma ( Rb ) phosphorylation and a corresponding increase in the availability of the ribosomal DNA transcription factor upstream binding factor ( UBF ) . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Rb 1 is required to enforce cell cycle checkpoints in response to DNA damage , but evidence for a direct role in the processing and repair of DNA lesions is lacking . ^^^ We observe that degradation of trapped TOP 2 cleavable complexes , liberation of DNA strand breaks , and repair of those breaks occurs more efficiently in cells expressing Rb 1 protein ( pRb ) . ^^^ Rb 1 mediated processing and repair of TOP 2 cleavable complexes is genetically separable from its ability to bind E2F and enforce DNA damage induced cell cycle checkpoints . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Human papillomavirus ( HPV ) DNA sequences have been found in RB tissue , suggesting a role of the viral infection with RB . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
BACKGROUND : The retinoblastoma ( Rb ) pathway , which governs cell cycle progression , is frequently genetically altered in cancer , causing deregulated expression of the E2F 1 transcription factor , which promotes DNA synthesis and cell cycle progression . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Together with our previous results , we found that gross RB 1 alterations were present in the peripheral blood DNA from 65 of 433 ( 15 % ) and 17 of 262 ( 6 . 5 % ) patients with bilateral or familial and isolated unilateral retinoblastoma , respectively . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Detailed analysis of mechanisms of genetic loss for specific tumor suppressor genes ( TSGs ; e . g . , RB 1 , APC and NF 1 ) indicates that TSG inactivation can occur by allelic loss of heterozygosity ( LOH ) , without any alteration in DNA copy number . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Rb inactivation leads to E2F1 mediated DNA double strand break accumulation . ^^^ Thus , Rb status is key to regulating both the proliferation promoting functions associated with E2F and for preventing DNA damage accumulation if E2F1 becomes deregulated . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Two types of DNA synthetic sites / replication factories can be distinguished at ultrastructural level : ( 1 ) electron dense structures replication bodies ( RB ) , and ( 2 ) focal replication sites with no distinct underlying structure replication foci ( RF ) . ^^^ For example , while DNA polymerase alpha , PCNA and hnRNP A 1 were diffusely spread throughout RB , hnRNP C1 / C2 was found only at the very outside . ^^^ Surprisingly , RB contained only small amounts of DNA . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
RhoB , encoding a small GTPase has been identified as a DNA damage inducible gene . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In contrast , LIF fails to promote increases in cyclin D mRNA / protein levels ; consequently , LIF induces DNA synthesis without promoting full phosphorylation of retinoblastoma protein ( Rb ) . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
To overcome these limitations , we developed a straightforward reverse transfection method for WERI Rb 1 cells wherein snap frozen DNA / polyethylenimine complexes are freeze dried on the surface of 48 well plates and stored in desiccator until cells are seeded for transfection . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Standard curves and serial dilutions of plasmid cloned mitochondrion and retinoblastoma ( RB 1 ) PCR products with known concentrations were generated to estimate the mtDNA and nuclear DNA ( nDNA ) copy numbers in each sample . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Rb is a key target of oncoproteins expressed by DNA tumor viruses , but RNA viruses are not known to regulate Rb function . ^^^ NS5B contains a conserved Leu 10 Cys / Asn 10 Asp motif that is homologous to Rb binding domains in the oncoproteins of DNA viruses . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Furthermore , MDM 2 ablation results in Rb accumulation and inhibition of DNA synthesis . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The basis for this relationship is traceable to , among other things , high level Oct 1 DNA binding activity in Rb defective cells , which represses the prototypical human MHC class 2 gene , HLA DRA . ^^^ Ordinarily , re establishment of Rb expression leads to elimination of , or substantially reduced Oct 1 DNA binding activity and to rescue of HLA DRA inducibility . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
At 24 h , both p 53 and Rb expression returned to baseline , suggesting collapse of DNA structure and spindle assembly checkpoints characteristic of mitotic catastrophe . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
A comparative study of the effects of alkali metal ions Li ( + ) , Na ( + ) , K ( + ) , Rb ( + ) , and Cs ( + ) on the liquid crystalline organization of high molecular weight calf thymus DNA using polarized light microscopy was performed . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Five polymorphic DNA regions from TP 53 , RB 1 , WT 1 , and NF 1 were systematically studied by PCR denaturing gradient gel electrophoresis . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
A combination of random amplified polymorphic DNA ( RAPD ) and eastern blotting analyses using anti ginsenoside Rb 1 and Rgl monoclonal antibodies was used for the identification of P . notoginseng , P . quinquefolius and P . japonicus . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Furthermore , a polymerase arrest assay indicated that formation the G quadruplex structure in the Rb gene acts as a barrier in DNA synthesis . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Rb has also been implicated in playing a role in a wide variety of cellular processes including DNA repair , cellular senescence , cell fate determination and apoptosis . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Yet , it is unclear whether early programs of apoptotic injury that involve membrane phosphatidylserine ( PS ) exposure and calreticulin expression as well as later phases of apoptotic injury with nuclear DNA injury require the critical modulation of Rb and E2F1 . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
DNA sequence analysis with other vertebrate Rb sequences demonstrates that the L . limanda Rb cDNA is highly conserved in regions of functional importance . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
These results demonstrate a mechanistic role for p 130 and compensatory roles for p 107 and RB in the long term senescence like growth arrest response of breast cancer cells to DNA damage . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We recently demonstrated that the DNA methyltransferase 1 ( DNMT 1 ) gene is an E2F target gene that is transcriptionally activated in cells lacking the retinoblastoma gene ( Rb / ) . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
MDM 2 also interacts with Rb through its central acidic domain and inhibits Rb function in part by blocking Rb E2F DNA complex formation . ^^^ Knockdown of MDM 2 results in accumulation of hypophosphorylated Rb and inhibition of DNA synthesis . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
In the present study , we use DNA microarray technology to determine whether differences in gene expression profiles occurred in the unaffected parents of patients with hereditary Rb relative to normal individuals . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
RB recruits a number of proteins , including HDACs , SWI / SNF complex , lysine methyl transferase ( SUV39H1 ) and DNA methyltransferase ( DNMT 1 ) , all of which negatively regulate E2F activity with RB . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
When mammalian cells contain damaged DNA , the p 53 tumor suppressor and the Rb family of transcriptional repressors work together to downregulate a large number of genes that encode proteins required for G 2 and M . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Association with Rb inhibits the DNA binding function of nucleolin and in consequence the interaction of nucleolin with the HPV 18 enhancer , resulting in Rb mediated repression of the HPV 18 oncogenes . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Binding of E2F with hypophosphorylated Rb resulted in inhibition of E2F activity and reduction of E2F regulated gene transcription , these genes being necessary for S phase entry and DNA synthesis . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Over expression of TRF 2 ( DeltaBDeltaC ) induced apoptosis and reduced tumourigenicity exclusively in JR 5 cells . p 53 and Rb status and apoptotic response to DNA damage did not seem to account for the different response of the two lines to TRF 2 inhibition . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Considering the localization of the C positive heterochromatin and of the telomeric sequences , the events leading to the Kenyan cytotype from an all telocentric condition probably included two steps : first , fusion without loss of heterochromatin and pericentromeric telomeric sequences ; second , the reduction of the C positive satellite DNA followed by the amplification of telomeric sequences in the C negative paracentromeric region of Rb chromosomes . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
This review will focus on the role of the pocket proteins ( PPs ) , Rb 1 ( retinoblastoma protein ) , retinoblastoma like 1 ( Rbl 1 also known as p 107 ) and retinoblastoma like 2 ( Rbl 2 also known as p 130 ) , in melanocyte homeostasis , with particular emphasis on their functions in the cell cycle and the DNA damage repair response . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
DNA extraction and polymerase chain reaction amplification for 11 microsatellite markers on chromosomes 11p13 , 13q14 , and 17p13 ( WT 1 , RB , p 53 ) were performed . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
We have shown previously that cyclin dependent kinases ( CDKs ) , along with their downstream effectors , Rb ( retinoblastoma ) and E2F / DP1 ( E 2 promoter binding factor / deleted in polyposis 1 ) , regulate neuronal death evoked by the DNA damaging agent camptothecin . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
Recent studies have indicated that Rb and Rb2 / p130 may be involved in cellular response to DNA damage events , by influencing the transcription of factors involved in DNA repair pathways . ^^^ In particular , evidences suggest that Rb loss and target gene deregulation impacts on the repair of UV induced pyrimidine pyrimidone photoproducts ( 6 4 PP ) by regulating the expression of several DNA damage factors involved in UV DNA damage repair processes , including proliferating cell nuclear antigen . ^^^ Ongoing studies are focused on the mechanisms by which Rb family genes drive cell cycle exit following DNA damage induction , and how Rb gene family ' s interaction with chromatin remodeling factors can influence DNA repair dynamics . . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
The pocket domain of the RB family proteins is dramatically hampered in its functions by the interference of a number of proteins produced by the small DNA viruses . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
A novel regulatory element in the dnmt 1 gene that responds to co activation by Rb and c Jun . ^^^ Rb , c Jun and dnmt 1 play critical roles in the process of cellular differentiation . ^^^ We demonstrate that a regulatory region of murine dnmt 1 contains an element which is responsible for transactivation by Rb and c Jun in P 19 embryocarcinoma cells which is not observed in Y 1 adrenocarcinoma cells . ^^^ During differentiation of P 19 cells , the induction of Rb and c Jun coincides with an increase of dnmt 1 mRNA . ^^^
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
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Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA
Interacting proteins: P26358 and P06400	Pubmed	SVM Score :0.0
NA