| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| We now show , using biologically active fusion proteins , that cyclin A can direct the binding of the cdc 2 like kinase subunit , p33cdk2 , to complexed DRTF 1 , containing either Rb or p 107 , as well as activate its histone H 1 kinase activity . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Three nuclear genes , c myc , RB , and cyclin A were found to be common key downstream targets along the cytokine induced growth suppressive pathways . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| It contains p 107 , a nuclear `` pocket ' ' protein with similarities in structure and protein binding properties to RB , and cyclin A , a cyclin believed to play a role in facilitating DNA replication . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| High resolution fast protein liquid chromatography ( FPLC ) revealed that the Rb protein co purified with a particular subset of the PDPK heterodimer , i . e . with a single species of the 58 kDa cyclinA doublet . ^^^ Moreover , the induction of the cyclin A subunit of PDPK in these synchronized MG 63 cells was found to be closely correlated with the cell cycle dependent phosphorylation of the Rb protein . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Cyclin A bound to the p 107 pocket , but not the RB pocket . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| The expression of both CDC 2 and cyclin A was dramatically downregulated upon terminal differentiation of neurons in vivo and in a neuronal precursor cell line , ST15A . p 53 mRNA expression was also downregulated but to a lesser extent ; RB mRNA levels were unchanged during neuronal differentiation . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| This interaction is modulated by adenovirus E1a , which sequesters the Rb protein and several other cellular proteins , including cyclin A , a molecule that undergoes cyclical accumulation and destruction during each cell cycle and which is required for cell cycle progression . ^^^ Cyclin A , which also complexes with DRTF 1 , facilitates the efficient assembly of the Rb protein into the complex . ^^^ This suggests a role for cyclin A in regulating transcription and defines a transcription factor through which molecules that regulate the cell cycle in a negative fashion , such as Rb , and in a positive fashion , such as cyclin A , interact . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| DRTF 1 is a cellular transcription factor which complexes with the retinoblastoma ( Rb ) gene product and cyclin A , an association modulated by certain viral oncogenes , such as adenovirus E1a . ^^^ Certain tissues , such as liver and brain , contain high levels of DRTF 1 during early embryonic stages but little in adult terminally differentiated tissue , in contrast to the thymus , which contains high levels of Rb complexed DRTF 1 but lacks associated cyclin A . ^^^ These data show that DRTF 1 is a group of transcription factors that share common DNA binding polypeptides and which complex with other non DNA binding proteins , such as the Rb protein and cyclin A , in a developmentally regulated and tissue dependent fashion . . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| E2F transcription factor is a target for the RB protein and the cyclin A protein . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Genes encoding cdk 1 ( p34cdc2 ) , cyclin A , cyclin B , and the tumor suppressor gene Rb are fundamental regulators of cell cycle progression which associate as a complex with the transcription factor E2F . ^^^ We have investigated levels of mRNA encoding cdk 1 ( p34cdc2 ) , cyclin A , cyclin B , Rb , GAPDH , c myc , and histone H 4 genes for sensitivity to okadaic acid in HeLa cells to determine if transformation altered their regulation . ^^^ When starved cells were subsequently exposed to 19 nM okadaic acid or refed 10 % serum , mRNA levels of cyclin A , cyclin B , cdk 1 , and Rb dramatically increased while mRNA levels for c myc and GAPDH were largely unaffected . ^^^ Despite the cell cycle phase specific block , elevated mRNA levels for cdk 1 , cyclin A , cyclin B , Rb , and suppression of H 4 mRNA levels were detected and persisted for at least 12 hr following okadaic acid removal . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Induction of the Cip1 / WAF1 promoter upon differentiation is abolished by expression of an adenovirus E1A oncoprotein ( d1922 / 947 ) , which is unable to bind p 105 Rb , p 107 , or cyclin A but which still binds the nuclear phosphoprotein p 300 . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| The Rb related p 107 protein can suppress E2F function independently of binding to cyclin A / cdk2 . ^^^ The interaction of the retinoblastoma susceptibility gene product ( Rb ) related p 107 protein with the E2F transcription factor in S phase cells facilitates the formation of a multicomponent complex also containing cyclin A and the p33cdk2 kinase . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Regulation of adenovirus 12 E1A transcription : E2F and ATF motifs in the E1A promoter bind nuclear protein complexes including E2F1 , DP 1 , cyclin A and / or RB and mediate transcriptional ( auto ) activation . ^^^ For HeLa , 293 , U 937 , and A 549 cells , participation of E2F 1 , DP 1 , cyclin A , and RB was involved in formation of some complexes only , assuming participation of factors different from E2F 1 or DP 1 in others . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| E2F 1 forms a heterodimer with DP 1 and binds to several cell cycle regulatory proteins , including the retinoblastoma family ( RB , p 107 , p 130 ) and cyclin A / CDK2 complexes . ^^^ Because the stable association of E2F 1 with cyclin A / CDK2 in vitro and in vivo did not require a DP 1 or RB binding domain and because the interactions could be reconstituted from purified components in vitro , we conclude that the interactions between cyclin A / CDK2 and E2F 1 are direct . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| We cite the evidence suggesting that the inhibition of B myb and cyclin A may contribute to the late G 1 arrest caused by TGF beta 1 and that these events may be linked through the actions of the product of the retinoblastoma susceptibility gene ( Rb ) or an Rb family member . . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| GST / E7 fusion proteins from all these HPV types associate with Rb 1 , p 107 and the cyclin A / CDK2 complex . ^^^ As has been shown for Rb 1 , the association with p 107 and Cyclin A was weaker for the ' low risk ' HPV 6 and 11 E 7 proteins as compared to ' high risk ' HPV 16 , 18 and 33 E 7 proteins . ^^^ In contrast the E 7 protein of the benign type HPV 1 bound Rb 1 , p 107 and cyclin A with the same affinity as the ' high risk ' E 7 proteins . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| In contrast , expression of cyclin E , cyclin A , and Rb phosphorylation were delayed when quiescent c myc heterozygous cells were stimulated to enter the cell cycle . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| The observed IFN alpha sensitive CDK 2 kinase activity was dependent on Cyclin E and cyclin D 1 but not on cyclin A and was physically associated with transcriptional factors : RB and E2F . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| CIP 1 encodes a novel 21 kd protein that is found in cyclin A , cyclin D 1 , cyclin E , and Cdk 2 immunoprecipitates . p21CIP1 is a potent , tight binding inhibitor of Cdks and can inhibit the phosphorylation of Rb by cyclin A Cdk 2 , cyclin E Cdk 2 , cyclin D 1 Cdk4 , and cyclin D 2 Cdk4 complexes . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Effect of tumor suppressors on cell cycle regulatory genes : RB suppresses p34cdc2 expression and normal p 53 suppresses cyclin A expression . ^^^ Our DNA sequencing data indicate that the promoter region of the p34cdc2 gene contains putative E2F like binding sites which are recognized by Rb and binding sites for c myb , Sp 1 , and ATF , and that the promoter region of the cyclin A gene contains binding sites for p 53 , Sp 1 , and ATF . ^^^ Our data support the idea that tumor suppressors modulate the expression of cell cycle regulatory genes : RB regulates p34cdc2 expression and p 53 regulates cyclin A expression . . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Regulation of E2F involves its interactions with other cellular proteins , including the retinoblastoma protein ( Rb ) , the Rb related protein p 107 , cyclin A , and cdk 2 . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| A number of the cellular proteins which associate to E1A have been identified : the retinoblastoma susceptibility protein ( Rb ) , the p 107 protein , cyclin A and the p33cdk2 kinase . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| The mammalian transcription factor E2F binds to several cellular proteins including Rb , p 107 , cyclin A , cyclin E , and p33cdk2 protein kinase in a stage specific manner during cell cycle . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| The association of E 7 with cyclin A appeared to be direct , not involving Rb 1 or other proteins . . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Two potentially oncogenic cyclins , cyclin A and cyclin D 1 , share common properties of subunit configuration , tyrosine phosphorylation and physical association with the Rb protein . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Utilizing precisely synchronized human MG 63 osteosarcoma cells , the present study demonstrates that cyclin A mRNA and protein are clearly expressed in late G 1 prior to S phase entry , as is cyclin A associated kinase activity and concomitant phosphorylation of the Rb protein . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| The fact that E1A proteins with deletions in CR 2 show a greatly reduced association with RB , cyclin A and p33cdk2 suggests that these associations are dispensable for E1A mediated transformation of NRK cells . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| We therefore focused on factors that are functionally related to activity of the principal cell cycle regulatory element of the histone H 4 promoter : CDC 2 , cyclin A , as well as RB and IRF related proteins . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Taken together with our previous studies showing adhesion dependent expression of cyclin A , they also establish the cell cycle basis for explaining the combined requirement for growth factors and the extracellular matrix in transit through the Rb checkpoint , entry into S phase , and anchorage dependent growth . . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Furthermore , the activity of the H2A . 1 promoter was also downregulated by overexpression of the RB related p 107 , a protein that has been detected in S phase specific protein complexes of cyclin A , E2F , and cdk 2 . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Using the cell line , we investigated the expression of cell cycle associated proteins including cdc 2 , cdk 2 , cyclin A , cyclin Dl , and Rb during entry into or withdrawal from the cell cycle . ^^^ The results indicate that down regulation of neither cdk 2 , cyclin A , cyclin D 1 , nor MAP kinase is necessary to arrest cells in G 0 , but that only Rb dephosphorylation and down regulation of cdc 2 are accompanied by an arrest of cell proliferation in G 0 in the cell line . . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Moreover , SCF significantly increased cellular expression of cyclin A , a regulatory subunit of cyclin dependent protein kinase ( CDK ) , and the ratio of phosphorylated / dephosphorylated retinoblastoma gene product ( RB protein ) . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Cells arrested in G 1 by the 5 Abl tyrosine kinase do not express cyclin A despite the hyperphosphorylation of RB . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Wild type RB protein and cyclin A were expressed in both cells . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| IFN gamma caused a rapid ( within 12 24 h ) down regulation of cyclin A , c myc , and cdc 2 proteins , as well as a disappearance of hyperphosphorylated forms of the retinoblastoma family proteins , Rb and p 130 . ^^^ The block in Rb phosphorylation and cyclin A expression was coordinately regulated during the same narrow window of G 1 . ^^^ Several mammary carcinoma cell lines demonstrated resistance to the growth inhibitory effects of IFN gamma and did not exhibit down regulation of cdc 2 and cyclin A expression or a change in hyperphosphorylation of Rb when treated with IFN gamma . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Despite the phosphorylation of RB / p107 / p 130 , the expression of cyclin A and its associated kinase activity is delayed in rapamycin treated N 3T3 cells . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| HS 18 cells showed no expression of the Rb protein and high levels of expression of E2F , cyclin A , cyclin E and CDK 2 . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| On the other hand , there was no significant difference in expression of proteins such as Rb , p 16 , cdk 4 , cdk 6 , cyclin A and cyclin D 1 between the normal and immortalized human fibroblasts . . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| In support of this hypothesis , a hyperactive mutant of Rb was found to partially reverse the inhibition of MCK expression by cyclin D 1 and by the combination of cyclin A and Cdk 2 . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| The increase in the level of p 21 ( WAF1 / CIP1 ) was associated with the disappearance of the rapidly migrating , activated form of cdk 2 from cell lysates , dephosphorylation of the retinoblastoma protein ( Rb ) , and a decrease in cyclin A levels . ^^^ These experiments demonstrate that stimulation of MCF 7 cells with bFGF , although resulting in up regulation of G 1 proteins responsible for mitogenic events , also induces a concomitant decrease in cyclin A levels and an increase in p 21 ( WAF1 / CIP1 ) mRNA and protein and results in inactivation of cdk 2 , dephosphorylation of Rb , and a segregation of PCNA to the G 1 cyclin complexes . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Formation of the early region 2 transcription factor 1 retinoblastoma protein ( E2F 1 RB ) transrepressor and release of the retinoblastoma protein from nuclear complexes containing cyclin A is induced by interferon alpha in U937V cells but not in interferon alpha resistant U937VR cells . ^^^ Interestingly , treatment of nuclear extracts from logarithmically growing U937V and U937VR cells with an antiserum against cyclin A that disrupts cyclin A containing complexes , led to the formation of the E2F 1 RB complex , suggesting the presence of under phosphorylated ( active ) RB , trapped in nuclear complexes that contain cyclin A . ^^^ This suggestion was supported by combined immunoprecipitation / western blot experiments that revealed a physical interaction between phosphorylated as well as under phosphorylated forms of RB and cyclin A complex ( es ) in U937V and U937VR cells . ^^^ We conclude that the missing induction of the E2F 1 RB transrepressor by IFN alpha and the failure to release RB from cyclin A containing complexes might contribute to the resistance of U937VR cells to the antiproliferative effects of IFN alpha . . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| We examined the expressions of RB protein and cyclin A protein in 13 small cell lung cancer ( SCLC ) lines and 14 non small cell lung cancer ( NSCLC ) lines by immunoblotting . ^^^ A positive correlation ( P = 0 . 0034 ) between expression of cyclin A and wild type RB protein was found by Fisher ' s exact probability test . ^^^ The positive correlation between the expressions of RB protein and cyclin A suggests that RB protein in most RB ( + ) lung cancer cell lines is a target of cyclin A dependent kinase and that the tumor suppressor function may be inactivated by phosphorylation . . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Recent results on the structures of two cyclins , cyclin A and cyclin H , and two transcription factor mediator proteins , TFIIB and the A pocket region of the retinoblastoma tumour suppressor protein ( Rb ) , show that they share domains with a strikingly similar alpha helical topology , despite remote sequence identity . . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| The irreversible growth arrest state in NHEKs is characterized by an accumulation of the hypophosphorylated forms of Rb and p 130 , with subsequent down regulation of levels of Rb , up regulation of p 130 and associated down regulation of E2F regulated genes such as cyclin A . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Compared to cogenic wild type mice , p 21 / mice demonstrated evidence of markedly accelerated hepatocyte progression through G 1 phase after PH : DNA synthesis , upregulation of cyclin A and PCNA , induction of cyclin D 1 and CDK 2 associated kinase activity , and appearance of a phosphorylated retinoblastoma protein ( Rb ) species occurred earlier in the p 21 / mice . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| In the search for new risk factors at the molecular and cellular levels , clinical data [ lymph node involvement ( LN ) and stage ] were used and 104 squamous cell lung carcinomas were analyzed by immuno histochemistry for expression of cyclin D 1 , cyclin A , cdk 2 , cdk 4 , RB , and E2F1 . ^^^ The results of the univariate analysis of all 8 factors showed that cyclin A and cdk 2 gave the best prognostic information , while no prognostic value could be found associated with cyclin D 1 , cdk 4 , RB and E2F1 . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| In PSM RB arrested G 1 cells , normal levels of cyclin E and cyclin E associated kinase activity were detected , but the expression of cyclin A was inhibited . ^^^ The ectopic expression of cyclin E restored cyclin A expression and drove the PSM RB expressing cells into S phase . ^^^ However , the S phase inhibitory activity of PSM RB could not be overcome by the coinjection of cyclin E or cyclin A expression plasmids . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| The inhibition of G 2 progression was correlated with increased contents both of total p 21 ( Waf 1 ) and of p 21 ( Waf 1 ) associated with cyclin A and with an inhibition of cyclin A and B 1 associated histone H 1 kinase activities measured in cell lysates , as well as with dephosphorylation of the RB protein . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Anchorage dependent expression of cyclin A in primary cells requires a negative DNA regulatory element and a functional Rb . ^^^ In the experiments reported here , we show that anchorage dependent expression of cyclin A ( 1 ) is reflected by the in vivo occupancy of a negative DNA regulatory element previously shown to be instrumental in the down regulation of cyclin A transcription in quiescent cells ( Cell Cycle Responsive Element : CCRE ) ( 2 ) requires a functional Rb but neither p 107 nor p 130 ( 3 ) mutation of the CCRE abolishes both adhesion dependent regulation and response to Rb . . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| We show that active RB acts to attenuate cyclin A promoter activity , and that overexpression of cyclin E reverses RB mediated repression of the cyclin A promoter . ^^^ Although cyclin A is an E2F regulated gene , and it has been long hypothesized that RB mediates cell cycle advancement through binding to E2F and attenuating its transactivation potential , cyclin E does not reverse dominant negative E2F mediated repression of the cyclin A promoter . ^^^ Although active RB repressed both cyclin A and two other paradigm E2F regulated promoters , only cyclin A transcription was restored upon co expression of cyclin E . ^^^ Additionally , we show that RB but not dominant negative E2F regulates the cyclin A promoter through the CCRE element . ^^^ These data identify cyclin A as a downstream target of RB mediated arrest . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| In contrast , the expression of cyclin D and E and the phosphorylation of Rb protein were not affected by ectopic expression of the drs gene , suggesting that an Rb independent downregulation of cyclin A is involved in the suppression of anchorage independent growth by means of the drs gene . . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| The decrease in RB expression and phosphorylation , which is essential in triggering DNA synthesis and Cyclin A expression , leads to a deficiency in transcriptionally active E2F complex formation after hepatectomy . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| The expression of these two RB related proteins was correlated with the mitotic index , apoptotic index , and percentages of Ki 67 ( + ) , cyclin A ( + ) , p 34 ( + ) , and cyclin B ( + ) cells . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Mitogenic signaling events involving cyclins D and E , Rb phosphorylation , and transcriptional activation of E2F responsive genes ( including cyclin E and cyclin A ) were unaffected in cells containing PAH damaged DNA . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Furthermore , the activity of CDK 2 associated cyclin kinase was decreased , which was accompanied by the reduction of cyclin D 1 , cyclin E , and cyclin A associated kinase activities , resulting in the hypophosphorylation of Rb protein . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Furthermore , ( 1 ) the arrest of G ( 1 ) S transition exerted by PC 3 was completely rescued by coexpression of cyclin D 1 but not by that of cyclin A or E ; ( 2 ) expression of PC 3 caused a significant down regulation of cyclin D 1 protein levels , also in Rb defective cells , accompanied by inhibition of CDK 4 activity in vivo ; and ( 3 ) the removal from the PC 3 molecule of residues 50 to 68 , a conserved domain of the PC3 / BTG / Tob gene family , which we term GR , led to a loss of the inhibition of proliferation as well as of the down regulation of cyclin D 1 levels . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Cyclin A expression is repressed in quiescent cells by E2F acting in conjunction with its pocket protein partners Rb , p 107 , and p 130 ; however , 5 Jun overrides this control , causing phosphorylated Rb and proliferation specific E2F p 107 complexes to persist after mitogen withdrawal . ^^^ Dephosphorylation of Rb and destruction of cyclin A nevertheless occur normally at mitosis , indicating that 5 Jun enables cells to rephosphorylate Rb and reaccumulate cyclin A without exogenous mitogenic stimulation each time the mitotic `` clock ' ' is reset . ^^^ Thus , 5 Jun overrides the mitogen dependence of S phase entry by deregulating Rb phosphorylation , E2F pocket protein interactions , and ultimately cyclin A cdk 2 activity . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| FGF 2 stimulation , however , did not lead to phosphorylation of the retinoblastoma protein ( Rb ) , CDK 2 activation , or expression of cyclin A . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Although introduction of PSM RB repressed E2F mediated transcription in both C33A and SAOS 2 cells , PSM RB failed to repress Cyclin A promoter activity in C33A . ^^^ Ectopic expression of PSM RB in SAOS 2 cells resulted in a decrease in both Cyclin A and Cdk 2 protein levels without affecting Cyclin E or Cdk 4 . ^^^ In contrast , over expression of PSM RB in C33A cells did not alter endogenous Cyclin A , Cyclin E , or Cdk 2 protein levels or impact Cdk 2 kinase activity , indicating that signaling from RB to down stream targets is abrogated in this cell line . ^^^ Co expression of AHR / BRG 1 with PSM RB attenuated Cyclin A and Cdk 2 expression as well as Cdk 2 associated kinase activity , resulting in cell cycle inhibition of C33A cells . ^^^ Importantly , ectopic expression of Cyclin A was able to reverse the arrest mediated by co expression of AHR / BRG 1 with PSM RB . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| However , the Rb hSWI / SNF complex persists and is sufficient to maintain repression of the cyclin A and cdc 2 genes , inhibiting exit from S phase . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Wild type but not Rb ( ) LT activated the cyclin A promoter under serum starvation conditions . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| In cell lines lacking BRG 1 , we noted a profound defect in RB mediated repression of the cyclin A promoter . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| TSA enhanced the protein expression of p 21 ( WAF 1 ) , CREB binding protein , cyclinE , cyclin A , Bak and Bax , while it reduced the expression of E2F 1 , E2F 4 , HDAC 1 , p 53 and hyperphosphorylated form of Rb . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Immunohistochemical expression of the p 53 , mdm 2 , p21 / Waf 1 , Rb , p 16 , Ki 67 , cyclin D 1 , cyclin A and cyclin B 1 proteins and apoptotic index in T cell lymphomas . ^^^ Fifty seven cases of T cell lymphomas ( TCL ) including 5 lymphoblastic ( T LBL ) and 52 peripheral TCL ( PTCL ) were analyzed by immunohistochemistry for the expression of p 53 , mdm 2 , p 21 , Rb , cyclin D 1 , cyclin A , cyclin B 1 , and Ki67 / MIB1 proteins and 39 / 52 PTCL were also analyzed for the expression of p 16 protein and for the presence of apoptotic cells by the TUNEL method . ^^^ Rb expression tended to be parallel to Ki 67 , cyclin A and cyclin B 1 expression in all but one case of nodal PTCL UC which displayed loss of RB expression . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| We also show that RB mediated PCNA inhibition was dependent on downregulation of CDK 2 activity , which was achieved through the downregulation of cyclin A . ^^^ Importantly , restoration of cyclin dependent kinase 2 ( CDK 2 ) cyclin A and thus PCNA activity partially restored S phase progression in the presence of active RB . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Immunohistochemical expression of p 53 , p21 / waf1 , rb , p 16 , cyclin D 1 , p 27 , Ki 67 , cyclin A , cyclin B 1 , bcl 2 , bax and bak proteins and apoptotic index in normal thymus . ^^^ The immunohistochemical expression of p 53 , p 21 , Rb , p 16 , cyclin D 1 , Ki 67 , cyclin A , cyclin B 1 , p 27 , bcl 2 , bax , and bak proteins and the apoptotic index ( Al ) were investigated in 20 normal thymuses ( 8 adults , 3 adolescents , 5 infants and 4 newborns ) . ^^^ The expressions of Rb , Ki 67 , cyclin A and cyclin B 1 were overlapping , being high in the cortex with a tendency for decreased expression toward the medulla . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Low expression of p 27 protein combined with altered p 53 and Rb / p16 expression status is associated with increased expression of cyclin A and cyclin B 1 in diffuse large B cell lymphomas . ^^^ The expression of the cyclin dependent kinase inhibitor ( CDKI ) p 27 protein was investigated in relation to ( 1 ) the expression of the cell cycle regulators p 53 , Rb and p 16 and ( 2 ) the proliferation profile as determined by the expression of Ki 67 , cyclin A , and cyclin B 1 in 80 cases of de novo diffuse large B cell lymphomas ( DLBCL ) . ^^^ Indeed , the analysis of the combined p27 / p53 / Rb / p16 expression status with respect to the proliferation profile showed that ( 1 ) three alterations in the combined p27 / p53 / Rb / p16 status ( i . e . , low / null P 27 expression , increased expression of p 53 , and decreased expression of Rb or p 16 ) were significantly correlated with increased expression of cyclin B 1 ( P = . 005 ) and ( 2 ) two or three alterations were significantly correlated with increased expression of cyclin A ( P = . 014 ) . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Reintroduction of either BRG 1 or Brm into A 427 or C33A cells restored RB mediated signaling to cyclin A to cause cell cycle arrest . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| This damage led to RB dephosphorylation / activation and subsequent RB dependent attenuation of cyclin A and CDK 2 activity . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| We propose that the observed reduction in Rb protein level occurs through Rb degradation via the ubiquitin / proteasome pathway , and is preceded by selective Rb phosphorylation by cyclin A / Cdk2 and cyclin B / Cdk1 . . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| This inhibition of proliferation is correlated with increases in expression levels of the cell cycle inhibitor p 27 ( Kip 1 ) , reductions in the expression of cyclin A and changes in the relative phosphorylation levels of Rb . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| A GST beta ( 3 ) endonexin fusion , but not the corresponding R5A / L7A mutant , inhibits phosphorylation of Rb protein by cyclin A / Cdk2 in vitro . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Addition of serum was followed by enhanced cyclin D 1 expression , decreased p 27 ( KIP 1 ) levels , hyperphosphorylation of Rb protein , induction of cyclin A and cyclin D 3 expression , and cell cycle progression into S phase . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| In cl 2 cells in semisolid culture , DEX suppressed the expression of P27Kip1 , whereas it stimulated the expression of cyclin A and hyperphosphorylated retinoblastoma ( Rb ) proteins . ^^^ Our results suggest that the stimulation of anchorage independent growth by DEX involves at least two regulatory mechanisms , i . e . , one that leads to the suppression of P27Kip1 protein without requiring cytoskeletal integrity , and another that requires cytoskeletal integrity , leading to stimulation of cyclin A and hyperphosphorylation of Rb protein . . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| To examine the hypothesis that abnormal cell cycle regulators act as the mutators contributing to genomic instability , the present study , based on primary tumor tissues from 71 patients with breast cancer , was performed to determine whether there was an association between aberrant expression of cell cycle regulators ( cyclin A , cyclin D 1 , cyclin E , RB 1 , p 21 , and p 27 ) and chromosomal instability . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| In contrast , PSM RB leads to a dramatic reduction in cyclin A protein levels by coordinate transcriptional repression and degradation . ^^^ These studies indicate that RB inhibits cell cycle progression by targeting CDK2 / cyclin A dependent events at the G1 / S transition to inhibit cell cycle progression . . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| LPTs failed to upregulate cyclin dependent kinase 4 and cyclin D 3 , but Rb phosphorylation and cyclin A and B 1 upregulation were induced by CD 2 engagement . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Importantly , arrested cells exhibited strong induction of p16ink4a , hypophosphorylated RB , and down regulation of cyclin A , suggesting that hSNF 5 signals upstream of RB to induce growth arrest . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Our results show that the expression of RB C pocket fragments not only induces delayed cell cycle progression , which is mediated by the down regulation of cyclin A , cyclin E , and E2F 1 , but also sensitizes cells to apoptosis through p 53 dependent pathways . . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| METHODS : The molecular expressions of Rb , cyclin A , cyclin E , CDK 2 and CDC 2 involved in S phase progression were investigated by westernblot in L 2 and Br 1 3pr 1 cells . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| In the present study 79 cases of de novo Diffuse Large B cell Lymphomas ( DLBCL ) were studied in order : a ) to analyse the expression of cyclin D 3 , cyclin E and cyclin D 1 in relation to other proliferative features ( expression of Ki 67 , cyclin A and cyclin B 1 ) , the apoptosis status and the expression of p 53 , Rb , p 16 and p 27 ; and b ) to determine whether distinct clusters of proliferation and apoptosis could be identified in DLBCL . ^^^ Combined alterations of the P 53 and the Rb / p16 / cyclin D 3 expression status were significantly associated with higher mean values of cyclin A ( p=0 . 023 ) and cyclin B 1 ( p=0 . 033 ) indicating that concurrent impairment of the p 53 and Rb 1 pathways induces increased tumour cell proliferation in DLBCL . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Therefore , 79 cases of de novo diffuse large B cell lymphomas were studied for the expression of bcl 6 and CD 10 proteins in relation to 1 ) the apoptotic index ; 2 ) the proliferation associated proteins Ki 67 , cyclin A , and cyclin B 1 ; and 3 ) the expression of the bcl 2 , p 53 , Rb , p 16 , and p 27 proteins . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Furthermore , the expression of some cell cycle and apoptosis related proteins , such as Bcl 2 , Rb , P 16 , caspase 3 , cyclin A and cyclin E , was altered in As ( 2 ) O ( 3 ) treated NB 4 cells . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| We find that active RB mediates histone deacetylation on cyclin A , Cdc 2 , topoisomerase IIalpha , and thymidylate synthase promoters . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Release of pre existing E2Fs from inactive complexes of E2F and hypophosphorylated retinoblastoma ( RB ) is the prevailing dogma for E2F transcriptional activation of target genes such as cyclin A . ^^^ Here we explored the hypothesis that new synthesis of E2F 1 is required for insulin like growth factor 1 ( IGF 1 ) to induce cyclin A accumulation and RB hyperphosphorylation , events that are targeted by tumor necrosis factor alpha ( TNFalpha ) to arrest cell cycle progression . ^^^ We first established that IGF 1 increases expression of cyclin A , causes hyperphosphorylation of RB , and augments the mass of E2F 1 in a time dependent manner . ^^^ Most important , this E2F 1 small interfering RNA also blocks the ability of IGF 1 to increase cyclin A accumulation and to hyperphosphorylate RB . ^^^ We next established that TNFalpha dose dependently inhibits IGF 1 induced phosphorylation of both RB and histone H 1 by cyclin A dependent cyclin dependent kinases . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| In rasREF cells treated with DE for 72 h in suspension culture ( a ) , the levels of cyclin D 1 , cyclin A , p 27 ( Kip 1 ) , and hyperphosphorylated Rb were decreased , but the levels of cdk 4 , cdk 6 , cdk 2 , p 16 ( INK4a ) , and p 21 ( Cip 1 ) were not affected . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| The protein responsible for CDK inactivation during mitotic exit in p21 / p27 null cells was the Rb family member , p 107 . p 107 bound to cyclin A 47 when p 21 and p 27 were absent , and cyclin A 47 CDK activity was not inactivated during the M to G 1 transition in p 21 ( / ) p 27 ( / ) p 107 ( / ) null fibroblasts . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Therefore , the bcl6 / CD10 / MUM1 / CD138 differentiation immunophenotypic profiles were studied in relation to ( a ) the apoptotic index , ( b ) the apoptosis associated bcl 2 family proteins bcl 2 , bcl xl , bax , bak , bad and bid , ( c ) the proliferation index ( Ki 67 ) and ( d ) the cell cycle proteins cyclin A , cyclin B 1 , cyclin D 3 , cyclin E , p 53 , Rb , p 16 and p 27 in 79 cases of diffuse large B cell lymphomas . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Ki 67 parameters , cell cycle regulators ( Rb , p 53 , Cyclin A , E and D , p 16 , p 21 , p 27 , and telomerase ) , and cellular differentiation products ( involucrin , CK 13 , CK 14 ) were compared in the basal zone as well as the deeper and upper halves of the epithelium . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Western blot showed loss of nuclear as well as cytoplasmic cyclin A and cyclin B protein after 2 AAF treatment , while the Rb protein level was markedly increased during G 1 . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Here , we describe methodologies for evaluation of three facets of RB action in the DNA damage checkpoint response : ( 1 ) transcriptional repression of E2F regulated genes ( cyclin A reporter assay ) ; ( 2 ) induction of cell cycle arrest ( Brd U incorporation assay ) ; and ( 3 ) inhibition of DNA double strand break accumulation ( phosphorylated histone H2A . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| In quiescent , estrogen deprived MCF 7 cells , estradiol did not induce a rapid activation of either the MAPK / ERK or phosphatidylinositol 3 kinase ( PI 3K ) / Akt pathway , whereas the entry into the cell cycle was documented by the successive inductions of cyclin D 1 expression , hyperphosphorylation of the retinoblastoma protein ( Rb ) , activity of the promoter of the cyclin A gene , and DNA synthesis . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| The aim of the present study was to assess expression of cyclin D 2 , Ki 67 , cyclin A , cyclin B 1 , cyclin D 1 , cyclin D 3 , cyclin E , p 53 , Rb , p 16 and p 27 proteins in order to gain further insight into the proliferation profile of cHL . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Western immunoblot was used to examine cell cycle protein expression and phosphorylation of the retinoblastoma protein ( Rb ) and cyclin A , both of which regulate cell cycle progression . ^^^ Notably , doxazosin inhibited phosphorylation of Rb and expression of cyclin A , both of which are necessary for cell cycle progression . ^^^ Doxazosin induced cell cycle inhibition appears to be at least in part due to an inhibition of mitogen induced Rb phosphorylation and cyclin A expression . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| In addition , coexpression of the N Ras protooncogene in thymic lymphomas induced by the N Ras oncogene is associated with increased levels of p 107 , p 130 and cyclin A and decreased levels of Rb . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| It has only one homolog of CDKA , CDKB , CDKD , cyclin A , cyclin B , cyclin D , cyclin H , Cks , Rb , E2F , DP , DEL , Cdc 25 , and Wee 1 . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| A dual mechanism of cyclin A transcriptional repression involves , on the one hand , promoter bound inhibitory complexes of E2F transcription factors and RB ( retinoblastoma ) family proteins , and on the other , chromatin directed histone deacetylase activity that is recruited to the cyclin A promoter early in the cell cycle in association with these RB proteins . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Low grade superficial papillary tumors induced by activated H ras had no detectable Rb family proteins ( Rb , p 107 , and p 130 ) and late cell cycle cyclins and kinases ( cyclin A , E , and CDK 1 ) , but had increased level of p 16 , p 53 , and MDM 2 . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| While much of large T function is related to its effects on tumor suppressors of the retinoblastoma susceptibility ( Rb ) gene family , we have previously shown that activation of the cyclin A promoter can occur through a non Rb dependent mechanism . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| By contrast , Smad 7 overexpression interfered with TGF beta 1 mediated attenuation of cyclin A and B levels , inhibition of cdc 2 dephosphorylation and CDK 2 inactivation , up regulation of p 27 , and the maintenance of the retinoblastoma protein ( RB ) in a hypophosphorylated state . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Furthermore , we show that 4 . 1B inhibits the proliferation of mammary epithelial cell lines by inducing a G 1 cell cycle arrest , characterized by decreased cyclin A expression and reduced Rb phosphorylation , and accompanied by reduced erbB 2 phosphorylation . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Cyclin A promoter assays showed that J domain function also contributes to large T transactivation that is independent of Rb . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Although the PKC effect was unrelated to the inhibition of cyclin D 1 expression , PKC activation significantly up regulated p 21 and down regulated Rb hyperphosphorylation and cyclin A expression . ^^^ Studies using luciferase reporters also revealed an essential role for PKCdelta in the PMA induced inhibition of Rb dependent cyclin A promoter activity . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| This amazing synergism was accompanied by synergistic modulation of E2F 1 and p 27 ( Kip 1 ) , which were reported to mediate the anticancer activities of lovastatin and troglitazone , respectively , and other cell cycle regulating proteins such as CDK 2 , cyclin A and RB phosphorylation status . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Activin maintained expression of p 21 and p 27 cyclin dependent kinase inhibitors involved in cell cycle control , enhanced expression of p 15 , reduced cyclin A expression , and reduced phosphorylation of the retinoblastoma ( Rb ) protein . ^^^ Smad 3 overexpression recapitulated activin induced p 15 expression and repression of cyclin A and Rb phosphorylation . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| The expression levels of cyclin dependent kinase 2 and cyclin A , as well as cyclin dependent kinase 1 and cyclin B , were decreased by TH , and after withdrawal not only did these regulators of Rb phosphorylation and mitosis increase in their expression but so too did the D 1 and D 3 cyclins . ^^^ This led to the hypothesis that TH , acting through increased type 5 somatostatin receptor , could activate the ERK pathway leading to cAMP response element binding protein dependent decreased expression of critical cell cycle proteins , specifically cyclin A , resulting in hypophosphorylation of Rb and its subsequent arrest of S phase progression . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Elevated RBBP 9 expression in males was paralleled by a pronounced dephosphorylation of RB and a significant drop of PCNA and cyclin A expression . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Levels of p 130 rose after drug treatment , and it was the primary RB family member recruited to the S phase promoters cyclin A and PCNA and G 2 M promoters cyclin B and cdc 2 , remaining present for the entire 8 day time period . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Both cyclin A and phophorylated RB levels were decreased in H 37 transfected cells , whereas expression of Bax protein was increased . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| Membrane depolarization induced by 50 mM KCl for 5 min significantly increased SH SY5Y cell numbers and thymidine incorporation at 24 h after depolarization , and increased the phosphorylation and expression of retinoblastoma protein ( RB ) , the activity of Cdk 2 ( without changing the activities of Cdk 4 and Cdk 6 ) , and the expressions of cyclin A and cyclin E . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| SUV39H1 cooperates with Rb to repress the cyclin E promoter , and in fibroblasts that are disrupted for SUV 39 , the activity of the cyclin E and cyclin A 2 genes are specifically elevated . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| These events were followed by a transient accumulation of cyclin E , stimulation of the associated histone H 1 kinase activity , a delayed accumulation of cyclin A 2 , and Rb hyper phosphorylation . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| We observed a gradual decrease of Retinoblastoma protein ( Rb ) phosphorylation and reduced expression of E2F target genes , like Cdc 2 and cyclin A 2 , during embryogenesis and in embryonic fibroblasts ( MEFs ) . ^^^ |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20248 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|