| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.58549516 |
| Here we present evidence that the cellular transcription factor PU . 1 can bind to both RB and TFIID . 0.58549516^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.8056711 |
| Previous analyses have shown that Sp 1 is one of three RCE binding proteins identified in nuclear extracts and that Rb functionally interacts with Sp 1 in vivo , resulting in the `` superactivation ' ' of Sp 1 mediated transcription . 0.8056711^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| Rb promotes differentiation of macrophages by opposing the inhibitory functions of Id 2 on the transcription factor PU . 1 , a master regulator of macrophage differentiation . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| Because the PRAD 1 , or cyclin D 1 , gene , a cell cycle regulator , has been implicated in a subgroup of benign parathyroid tumors , we examined the possibility that another cell cycle regulator with possible functional links to PRAD 1 , the retinoblastoma tumor suppressor gene ( RB ) , might be involved in the molecular pathogenesis of parathyroid carcinoma . ^^^ Parathyroid carcinomas from 9 patients and adenomas from 21 were studied for evidence of tumor specific loss of RB gene DNA ( allelic loss ) by analysis of four DNA polymorphisms and for evidence of altered expression oF RB protein by immunohistochemical staining . ^^^ All of 11 specimens from 5 patients with parathyroid carcinoma and informative DNA patterns and 1 of 19 specimens from 19 patients with parathyroid adenoma and informative DNA patterns lacked an RB allele . ^^^ Fourteen of 16 specimens ( 88 percent ) from the nine patients with carcinoma had abnormal expression of RB protein ( a complete or predominant absence of nuclear staining for the protein ) . ^^^ None of the 19 adenomas , including the tumor with loss of an RB allele , had unequivocally abnormal staining for RB protein . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| Five fiber rich food sources , wheat bran ( WB ) , rice bran ( RB ) , oat fiber ( OF ) , apple fiber ( AF ) , and tomato fiber ( TF ) and their isolated insoluble fiber fractions were evaluated in vitro for their binding capacity for zinc ( Zn ) and copper ( Cu ) . ^^^ Total Cu bound ranged from 3687 micrograms / g for OF to 8019 micrograms / g and 8073 micrograms / g for WB and AF , whereas , bound Zn levels varied from 1213 micrograms / g for OF to 7121 micrograms / g and 7166 micrograms / g for WB and RB , respectively . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| To understand the possible role of DCCD in inhibiting the protonogenic reactions of cytochrome bc ( 1 ) complex , we investigated the effect of DCCD modification on flash induced electron transport and electrochromic bandshift of carotenoids in Rb . sphaeroides chromatophores . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| By comparing the relative distribution of ( 10 ) B , Rb ( xylem to phloem transfer marker ) and Sr ( xylem marker ) in the ear and the flag leaf , the distribution of ( 10 ) B resembled that of Rb more than Sr . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| The synthesis of the alkali metal salts of 3 , 4 , 5 tris ( n alkan 1 yloxy ) benzoic acid [ ( 3 , 4 , 5 ) nG 1 CO ( 2 ) M , where n is the number of methylenic units in the alkane group for n=12 , 14 , 16 , 18 and M=Li , Na , K , Rb , Cs ] is described . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| Interferometric cross referencing to metrologic Rb 3S 5D two photon transitions allowed measurement of the transition isotope shift and hyperfine splitting in the 3S state with precision at the 30 kHz level . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| Photooxygenation reaction of terpinene ( 1 ) in the presence of tetraphenyl porphine ( TPP ) or Rose Bengal ( RB ) gave 2 , 5 dihydroperoxy 1 isopropylene 4 methylene cyclohexane ( 2 ) , 3 , 6 dihydroperorxy 3 isopropyl 6 methyl 2 , 4 cyclohexadiene ( 3 ) and 1 , 2 & 4 , 5 diepoxy 1 isopropyl 4 methylcyclohexane ( 4 ) . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| Chemical bond in the cyclic anions P 6 4 and As 6 4 : synthesis , crystal structure , and electron localization function of { Rb [ 18 ] crown 6 ) } 2 Rb2As6 . 6 NH 3 . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| GOOSECOID inhibits erythrocyte differentiation by competing with Rb for PU . 1 binding in murine cells . ^^^ The N terminal domain of PU . 1 is the region recognized by the retinoblastoma protein ( Rb ) , a tumor suppressor gene presumably involved in erythroid differentiation . ^^^ We show that GSC competitively inhibits binding of Rb to PU . 1 . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| Five days later , the cultures were analyzed for cell survival , activation , DNA synthesis , and expression of EBV encoded and of cellular regulatory proteins retinoblastoma ( Rb ) , p 53 , recombinant sequence binding protein ( RBP ) Jk , and PU . 1 . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| Moreover , by using a GAL 4 Sp1 fusion protein , we have directly demonstrated that Rb positively regulates Sp 1 transcriptional activity in vivo . ^^^ These results indicate that Rb can function as a positive regulator of transcription and that Sp 1 is one potential target , either directly or indirectly , for transcriptional regulation by Rb . . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| Here , we report that GAL 4 Rb can repress transcription mediated by either Sp 1 , AP 1 , or p 53 , dependent upon the presence of both the GAL 4 DNA binding domain and GAL 4 binding sites . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| Since Rb is a regulator of E2F and Sp 1 mediated transcription , we have examined the effect of overexpression of cyclins on transcription mediated by E2F dependent adenovirus E 2 promoter and by Sp 1 in a cotransfection assay in 3T3 cells . ^^^ In contrast , cyclin D 1 was able to repress specifically Sp 1 mediated transcription through an Rb independent pathway . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| Evidence for the role of Rb in mediating Sp 1 binding in aortic smooth muscle cells . ^^^ Addition of retinoblastoma gene product ( Rb ) antibody mimicked the effect of IGF 1 in gel shift analysis , suggesting that Sp 1 binding may be regulated by an inhibitor normally associated with Rb . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| The retinoblastoma gene product RB stimulates Sp 1 mediated transcription by liberating Sp 1 from a negative regulator . ^^^ The nucleotide sequence of the retinoblastoma control element ( RCE ) motif , GCCACC or CCACCC , and the Sp 1 consensus binding sequence , CCGCCC , can confer equal responsiveness to RB . ^^^ Here , we report that RB activates transcription of the c jun gene through the Sp 1 binding site within the c jun promoter . ^^^ Preincubation of crude nuclear extracts with monoclonal antibodies to RB results in reduction of Sp 1 complexes in a mobility shift assay , while addition of recombinant RB in mobility shift assay mixtures with CCL 64 cell extracts leads to an enhancement of DNA binding activity of SP 1 . ^^^ These results suggest that RB is directly or indirectly involved in Sp 1 DNA binding activity . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| The human RB gene is considered as a housekeeping gene with no characteristic TATA or CAAT elements in its promoter region , but the sequence between 206 and 185 bases upstream of the initiation codon , essential for RB promoter activity , contains putative Sp 1 and ATF recognition sites . ^^^ We have previously reported that point mutations in this region , causing low penetrance retinoblastomas , completely reduced RB promoter activity , and that a nuclear factor , named RBF 1 ( retinoblastoma binding factor 1 ) , could specifically bind to this sequence , overlapping Sp 1 recognition sequence . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| Our DNA sequencing data indicate that the promoter region of the p34cdc2 gene contains putative E2F like binding sites which are recognized by Rb and binding sites for c myb , Sp 1 , and ATF , and that the promoter region of the cyclin A gene contains binding sites for p 53 , Sp 1 , and ATF . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| Activity of the RB 1 promoter was found to be stimulated in retinoic acid induced differentiated P 19 cells compared to undifferentiated P 19 ; this stimulation required intact Sp 1 and ATF sites but not the putative E2F / DRTF1 binding site . ^^^ Our results indicate that basal level of RB 1 expression is governed by Sp 1 and ATF . . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| Sp 1 binds promoter elements regulated by the RB protein and Sp 1 mediated transcription is stimulated by RB coexpression . ^^^ Moreover , we show that Sp 1 mediated transcription is stimulated by the transient coexpression of RB protein . ^^^ We conclude from these observations that RB may regulate transcription in part by virtue of its ability to functionally interact with Sp 1 . . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| Electromobility shift assays demonstrated that SP 1 does not bind to oligomers from this region of the RB 1 promoter but bandshifts were seen for an , as yet , unidentified protein ( s ) which was not seen using an oligomer containing the G > C mutation . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| Rb represses some genes by sequestering or inactivating the positive transcription factor E2F and seems to activate some others by interacting with factors like Sp 1 or ATF 2 . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| Deletion analysis of the pregenomic promoter indicated that the region between nt 96 and 66 , which contains two Sp 1 binding sites , is responsible for activation by Rb . ^^^ Mutation of the Sp 1 binding sites abolished activation of the pregenomic promoter by Rb in the heterologous and natural promoter context . ^^^ Therefore , our results suggest that Rb can activate the HBV pregenomic promoter through the Sp 1 binding sites . . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| A unique cathepsin like protease isolated from CV 1 cells is involved in rapid degradation of retinoblastoma susceptibility gene product , RB , and transcription factor SP 1 . ^^^ Transcription factor , SP 1 , and retinoblastoma susceptible gene product , RB , are substrates of SPase while other nuclear factors such as c Jun and c Fos are not . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| We have also previously determined that Sp 3 and Sp 1 , two members of the Sp family of transcription factors , encode the 115 and 95 kDa RCPs respectively and that Rb stimulates Sp1 / Sp3 mediated transcription in vivo . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| We previously reported two oncogenic point mutations present in the RB ( retinoblastoma ) gene promoter region , found at consensus Sp 1 and ATF sites , respectively , and in two separate hereditary RB families . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| Furthermore , we have identified endogenous ( m ) Cp ( m ) CpG methylation of an Sp 1 site in the CpG island promoter of the retinoblastoma ( Rb ) gene by genomic sequencing . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| The promoter region of rhoB harbors regulatory elements such as TATA , CAAT , and Sp 1 boxes but not consensus sequences for AP 1 , Elk 1 , or c Jun / ATF 2 . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| This proteolytic enzyme selectively degraded nuclear proteins such as retinoblastoma susceptibility gene product , RB , and transcription factor , SP 1 . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| It has been suggested that Sp 1 is a potential target for transcriptional regulation by the cell cycle regulator retinoblastoma protein ( Rb ) , and so we have explored this possibility using the hamster dhfr gene as a model . ^^^ By the use of DNA probes from the hamster dhfr gene promoter , containing the most proximal GC box ( minimal promoter ) , and nuclear extracts from cultured hamster cells ( CHO K 1 ) , we show that polyclonal and monoclonal antibodies against Rb supershift the binding of Sp 1 . ^^^ Nuclear extract immunoprecipitation with anti Rb followed by Western analysis using anti Sp 1 also shows that Rb is complexed to Sp 1 . ^^^ Complementary Immunoprecipitation / WB analysis shows both forms of Rb protein in the anti Sp 1 immunoprecipitates . ^^^ Moreover , nuclear extract immunodepletion of Rb abolishes Sp 1 gel shift . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| Sp 1 mediated transcription of the Werner helicase gene is modulated by Rb and p 53 . ^^^ These findings suggest that WRN expression , like that of other housekeeping genes , is directed mainly by the Sp 1 transcriptional control system but is also further modulated by transcription factors , including Rb and p 53 , that are implicated in the cell cycle , cell senescence , and genomic instability . . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| We have previously shown that Sp 1 mediated transcription is stimulated by Rb and repressed by cyclin D 1 . ^^^ The stimulation of Sp 1 transcriptional activity by Rb is conferred , in part , through a direct interaction with the TBP associated factor TAF ( 2 ) 250 . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| The methylation sensitive endonuclease SacII cuts twice within a key hairpin loop structure in the RB promoter that contains binding sites for AP 1 , Sp 1 and RBF 1 . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| Moreover , the hyperphosphorylated retinoblastoma protein ( Rb ) increased in c jun expressing cells , indicating that phosphorylated Rb may play a role in regulating Sp 1 to repress p 21 expression . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| The distribution of WRNp and the expression of the transcription factors regulating WRN gene expression SP 1 , AP 2 , and retinoblastoma protein ( Rb ) were studied in the various human organs by immunohistochemical and immunoblot analyses . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| Using DNA affinity precipitation assays ( DAPA ) , we show that this complex contains SP 1 , NF 1 , Rb and HDAC 1 proteins . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| Moreover , Rb phosphorylation , which lies immediately downstream of the cyclin D 1 Cdk4 p21WAF1 / Cip1 complex , was blocked either by Sp 1 overexpression or antisense p21WAF1 / Cip1 . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| Moreover , immunoblot and immunoprecipitation analysis showed that the level of hyperphosphorylated retinoblastoma protein ( Rb ) is increased and the protein is physically associated with Sp 1 in PDGF treated cells , indicating that phosphorylated Rb may play a role in regulating Sp 1 to activate p 21 expression . . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| SPase is a cysteine protease isolated from an African green monkey kidney cell line , CV 1 , and has selective cleavage activity toward transcription factor SP 1 and retinoblastoma susceptibility gene product RB . ^^^ Bacterially expressed SPase protein exhibited proteolytic activity toward SP 1 and RB proteins . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| RESULTS : From a total of 345 screened TFs , 7 activity differential TFs were found , of which 5 showed increased activity , including p 53 , hypoxia inducible factor 1 alpha ( HIF 1alpha ) , signal transducer and activator of transcription 3 ( Stat 3 ) and Sp 1 , and 2 showed decreased activity including Rb and Smad 3 . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| Using 125I labelled rabbit IgG ( Rb IgG ) or sheep IgG ( Sh IgG ) it was possible to quantitate the amount of IgG coupled to plastic surfaces , or binding to SpA coated surfaces . ^^^ By using 125I labelled ( As 4 ) Rb IgG it was also possible to estimate the amount of antibody bound to SpA . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| Twenty five mouse lung tumors induced by a single urethan treatment in female A / J , BALB / c , and ( A / J 10 C3H / He ) F 1 ( AC 3 ) mice were analyzed for the presence of mutations at codon 61 of the Ki ras gene and for the expression of the surfactant protein A ( SP A ) , retinoblastoma ( Rb ) , growth arrest specific 3 ( gas 3 ) , p 53 , c myc , and thymidylate synthase ( TS ) genes . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| Radiolabeled GST Rb 152 StrepTag was brought in close proximity to the SPA scintillant beads through the interaction between StrepTag and streptavidin , resulting in the emission of light from beads . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| In the presence of calcium , a high mobility complex of SP A and [ 3H ] Rb LPS ( Rb mutant of LPS from Escherichia coli strain LCD 25 ) micelles was formed , as detected by sucrose density gradients . ^^^ |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P17947 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|