| Interacting proteins: P15172 and P06400 |
Pubmed |
SVM Score :0.0 |
| The present study characterises the modulation of retinoblastoma ( Rb 1 ) mRNA levels during myogenic differentiation of the murine C 2 cell line and provides evidence that the muscle specific regulatory factor MyoD enhances Rb 1 gene transcription . ^^^ We demonstrate that MyoD mediates the transactivation of a CAT construct whose expression is driven by the human Rb 1 gene promoter , and that this is not a consequence of direct binding of MyoD to an E box DNA sequence motif present in the Rb 1 promoter sequences . ^^^ In addition we have tested the capability of several MyoD mutant proteins of inducing the Rb 1 promoter CAT construct . ^^^ Our results indicate that the MyoD function required for induction of Rb 1 promoter activity is distinct from its myogenic function . . ^^^ |
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| Interacting proteins: P15172 and P06400 |
Pubmed |
SVM Score :0.0 |
| MyoD induced cell cycle arrest is associated with increased nuclear affinity of the Rb protein . ^^^ In studying the mechanism through which the myogenic determination protein MyoD prevents entry into the S phase of the cell cycle , we have found a relationship between MyoD and the retinoblastoma ( Rb ) tumor suppressor protein . ^^^ However MyoD injection did result in a block in the increase in Rb extractibility normally seen in late G 1 phase cells . ^^^ As this phenomenon is associated with the hyperphosphorylation of Rb at this point in the cell cycle and is correlated with progression into S phase , this provides further evidence that MyoD blocks the cycle late in G1 . . ^^^ |
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| Interacting proteins: P15172 and P06400 |
Pubmed |
SVM Score :0.0 |
| Both p 300 and Rb family protein binding regions of E1A are required for the repression of muscle gene expression , which is regulated by MyoD family transactivators . ^^^ |
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| Interacting proteins: P15172 and P06400 |
Pubmed |
SVM Score :0.0 |
| Furthermore , co transfection with the RB , which inhibits the transcription activity of E2F1 , can also repress E2F1 inhibition of myoD transactivation . ^^^ |
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| Interacting proteins: P15172 and P06400 |
Pubmed |
SVM Score :0.0 |
| In the antisense Rb clone cultured in differentiation medium , the amounts of MyoD and myogenin mRNA were markedly decreased on the 2nd day of culture in the differentiation medium . ^^^ |
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| Interacting proteins: P15172 and P06400 |
Pubmed |
SVM Score :0.0 |
| MyoD can induce PyLT expressing myoblasts to accumulate RB , p 21 , and muscle specific genes but is unable to induce G 0 ( 0 ) arrest . ^^^ |
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| Interacting proteins: P15172 and P06400 |
Pubmed |
SVM Score :0.0 |
| The inhibition of MyoD family function by HBP 1 required binding to RB and / or p 130 . ^^^ Thus , we postulate that the relative ratio of RB to HBP 1 may be one signal for activation of the MyoD family . ^^^ |
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| Interacting proteins: P15172 and P06400 |
Pubmed |
SVM Score :0.0 |
| The genes for p 21 , MyoD , Myogenin and RB were activated , and p 27 gene was repressed under the proteasome inhibition , suggesting the transcriptional regulation of these genes linked to the proteasome activity . ^^^ |
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| Interacting proteins: P15172 and P06400 |
Pubmed |
SVM Score :0.0 |
| Here we report that , at the onset of differentiation , activation by MyoD of the Rb , p 21 , and cyclin D 3 genes occurs in the absence of new protein synthesis and with the requirement of the p 300 transcriptional coactivator . ^^^ |
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| Interacting proteins: P15172 and P06400 |
Pubmed |
SVM Score :0.0 |
| The aberrant levels of Ras activity associated with loss of pRb may be responsible for the differentiation defects in Rb deficient cells , because suppression of Ras activity in Rb ( / ) fibroblasts restores the transactivation function of MyoD and the expression of a late marker of skeletal muscle differentiation . ^^^ |
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| Interacting proteins: P15172 and P06400 |
Pubmed |
SVM Score :0.0 |
| Selection of a dominant negative retinoblastoma protein ( RB ) inhibiting satellite myoblast differentiation implies an indirect interaction between MyoD and RB . ^^^ This unique dominant negative form of RB allows us to genetically determine if MyoD and RB associate in vivo . ^^^ The fact that the dominant negative RB inhibits myogenic differentiation in the presence of nonlimiting concentrations of either RB or MyoD suggests that these two proteins do not directly interact . ^^^ Therefore , E2F1 is a potential mediator of the dominant negative inhibition of MyoD by CB RB during satellite cell differentiation . ^^^ |
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| Interacting proteins: P15172 and P06400 |
Pubmed |
SVM Score :0.0 |
| A novel Rb and p 300 binding protein inhibits transactivation by MyoD . ^^^ Repression of skeletal muscle restricted genes was mediated by a block to transactivation by MyoD independent of G ( 1 ) exit and , surprisingly , was potentiated by a mutation that prevents EID 1 binding to Rb . ^^^ Thus , EID 1 binds both Rb and p 300 and is a novel repressor of MyoD function . . ^^^ |
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| Interacting proteins: P15172 and P06400 |
Pubmed |
SVM Score :0.0 |
| The transduction of exogenous TP 53 or Rb genes in p 53 defective myoblasts rescues MyoD activity and differentiation potential . ^^^ |
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| Interacting proteins: P15172 and P06400 |
Pubmed |
SVM Score :0.0 |
| In particular , in the presence or absence of dominant negative BRM or BRG 1 , MyoD was able to activate expression of p 21 , cyclin D 3 , and Rb , all of which are critical for cell cycle withdrawal in the G1 / G0 phase of the cell cycle . ^^^ |
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| Interacting proteins: P15172 and P06400 |
Pubmed |
SVM Score :0.0 |
| We investigated the mechanism leading to cyclin E accumulation when cultured mouse myoblasts , lacking functional Rb because of sequestration or deletion , are exposed to differentiating conditions ( mitogen subtraction and cell cell contact ) , which activate MyoD and normally downregulate factors involved in cell division . ^^^ After excluding that stabilization might account for the observed cyclin E mRNA accumulation , we found an induction of the cyclin E promoter that correlated with E2F activity upregulation and depended on both MyoD activation and Rb inactivation . ^^^ Analyses of the E2F1 promoter activity , in normal and Rb deficient fibroblasts converted by MyoD , identified a MyoD function stimulating E2F1 expression . ^^^ Its effects , although not indispensable for myogenesis , presumably contribute to raise the concentration of Rb E2F1 transcription repressing complexes , since MyoD strongly induces also Rb in differentiating myocytes . ^^^ In the absence of Rb , however , the induced E2F1 is left with only its activating role , reversing the normal effect of this MyoD function . . ^^^ |
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| Interacting proteins: P15172 and P06400 |
Pubmed |
SVM Score :0.0 |
| We proposed that SWI / SNF enzymes are required for the induction of all muscle specific gene expression by MyoD , Myf 5 , and MRF 4 , whereas induction of the cell cycle regulators , p 21 , Rb , and cyclin D 3 occurred independently of SWI / SNF function . . ^^^ |
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| Interacting proteins: P15172 and P06400 |
Pubmed |
SVM Score :0.0 |
| MyoD induces apoptosis in the absence of RB function through a p 21 ( WAF 1 ) dependent re localization of cyclin / cdk complexes to the nucleus . ^^^ During differentiation of skeletal myoblasts , MyoD promotes growth arrest through the induction of the cdk inhibitor p 21 and the accumulation of hypophosphorylated RB protein . ^^^ Here we show that exogenous MyoD induces apoptosis in several cell backgrounds sharing RB inactivation . ^^^ The inability of MyoD to induce apoptosis in a p 21 null background , highlights a requirement of p 21 in RB regulated apoptosis during myogenesis . ^^^ These results establish a novel link between MyoD , p 21 and RB during myogenesis , providing new insights into the antagonism between muscle differentiation and loss of RB function . . ^^^ |
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| Interacting proteins: P15172 and P06400 |
Pubmed |
SVM Score :0.0 |
| MyoD stimulates RB promoter activity via the CREB / p300 nuclear transduction pathway . ^^^ The induction of RB gene transcription by MyoD is a key event in the process of skeletal muscle differentiation , because elevated levels of the retinoblastoma protein are essential for myoblast cell cycle arrest as well as for the terminal differentiation and survival of postmitotic myocytes . ^^^ We previously showed that MyoD stimulates transcription from the RB promoter independently of direct binding to promoter sequences . ^^^ Here we demonstrate that stimulation by MyoD requires a cyclic AMP responsive element ( CRE ) in the RB promoter , bound by the transcription factor CREB in differentiating myoblasts . ^^^ Biochemical and functional evidence indicates that in differentiating myoblasts , MyoD becomes associated with CREB and is targeted to the RB promoter CRE in a complex also containing the p 300 transcriptional coactivator . ^^^ |
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| Interacting proteins: P15172 and P06400 |
Pubmed |
SVM Score :0.0 |
| Additionally , Rb ( / ) ; N ras ( / ) muscle shows a restoration in the expression of the late muscle specific gene MCK , and this correlates with a significant potentiation of MyoD transcriptional activity in Rb ( / ) ; N ras ( / ) , compared to Rb ( / ) myoblasts in culture . ^^^ |
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| Interacting proteins: P15172 and P06400 |
Pubmed |
SVM Score :0.0 |
| RB can form complexes with E2F and MyoD in vivo , and complexes with a number of other transcription factors have also been demonstrated in vitro . ^^^ |
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| Interacting proteins: P15172 and P06400 |
Pubmed |
SVM Score :0.0 |
| In vivo expression patterns of MyoD , p 21 , and Rb proteins in myonuclei and satellite cells of denervated rat skeletal muscle . ^^^ Using immunohistochemical approaches , we characterized cell types expressing MyoD , p 21 , and Rb and the relationship among these factors in the myonucleus of denervated muscles . ^^^ In addition , we quantitatively examined the time course changes and expression patterns among distinct myofiber types of MyoD , p 21 , and Rb during denervation . ^^^ Denervation induced MyoD expression in myonuclei and satellite cell nuclei , whereas p 21 and Rb were found only in myonuclei . ^^^ Furthermore , coexpression of MyoD , p 21 , and Rb was induced in the myonucleus , and quantitative analysis of these factors determined that there was no difference among the three myofiber types . ^^^ |
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| Interacting proteins: P15172 and P06400 |
Pubmed |
SVM Score :0.0 |
| A bioinformatic array analysis of regenerating Emd null muscle revealed abnormalities in cell cycle parameters and delayed myogenic differentiation , which were associated with perturbations to transcriptional pathways regulated by the retinoblastoma ( Rb 1 ) and MyoD genes . ^^^ The inappropriate modulation of Rb1 / MyoD transcriptional targets was associated with up regulation of Rb 1 , MyoD and their co activators / repressors transcripts , suggesting a compensatory effort to overcome a molecular block to differentiation at the myoblast / myotube transition during regeneration . ^^^ This compensation appeared to be effective for MyoD transcriptional targets , although was less effective for Rb 1 targets . ^^^ Surprisingly , Lmna null muscle did not show the same perturbations to Rb and MyoD dependent pathways . ^^^ |
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| Interacting proteins: P15172 and P06400 |
Pubmed |
SVM Score :0.0 |
| Nuclear envelope dystrophies show a transcriptional fingerprint suggesting disruption of Rb MyoD pathways in muscle regeneration . ^^^ A high proportion of top ranked and validated transcripts were components of the same transcriptional regulatory pathway involving Rb 1 and MyoD during muscle regeneration ( CRI 1 , CREBBP , Nap1L1 , ECREBBP / p300 ) , where each was specifically upregulated in EDMD . ^^^ We propose that key interactions between the nuclear envelope and Rb and MyoD fail in EDMD at the point of myoblast exit from the cell cycle , leading to poorly coordinated phosphorylation and acetylation steps . ^^^ |
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| Interacting proteins: P15172 and P06400 |
Pubmed |
SVM Score :0.0 |
| We used the semiquantitative high throughput method MethyLight to analyze a gene panel comprising ARF , CDKN2B , RB 1 , APC , CDH 1 , ESR 1 , GSTP 1 , TGFBR 2 , THBS 1 , TIMP 3 , PTGS 2 , CTNNB 1 , CALCA , MYOD 1 and HIC 1 . ^^^ |
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| Interacting proteins: P15172 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P15172 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P15172 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P15172 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15172 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15172 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15172 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15172 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15172 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15172 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15172 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15172 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15172 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15172 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
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