Pubmed abstracts for Protein-Protein Interaction search result :


Interacting proteins: P06400 and P05412 Pubmed SVM Score :1.3059634
The physical interactions between RB and c Jun were demonstrated by co immunoprecipitation of these two proteins using anti c Jun or anti RB antisera , glutathione S transferase affinity matrix binding assays in vitro , and electrophoretic mobility shift assays . 1.3059634^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :1.1189467
Here we show that Rb can physically interact with c Jun NH ( 2 ) terminal kinase / stress activated protein kinase ( JNK / SAPK ) , thereby inhibiting intracellular signals mediated by JNK / SAPK . 1.1189467^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
We performed experiments to determine the effects of ionizing radiation exposure on expression of genes such as beta actin , c fos , histone H 4 , c myc , c jun , Rb , and p 53 after exposure of Syrian hamster embryo ( SHE ) cells to the protein synthesis inhibitor cycloheximide . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
RESULTS : Negative growth control elements , including transcriptional repressors ( WT 1 , junB , myn , HNF 1 , p 53 , RB , Dr 1 , gas 2 , gadd 153 ) were induced 1 . 7 to 4 . 7 fold within 1 hour after uninephrectomy , as were positive growth control elements ( egr 1 , c jun , cyclin C , cyclin E ; 1 . 9 to 4 . 2 fold induction ) . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
Cells en route to apoptosis are characterized by the upregulation of c fos , c myc , c jun , cdc 2 , and RB phosphorylation , resembling events of early cell cycle traverse . ^^^ Investigating the regulation of early cell cycle genes during this process , we found that c myc , c jun , c fos , and cdc 2 protein presence is induced after serum deprivation , when the phosphorylated form of the RB protein also appears . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
Here we co inject RB protein with c myc , EJ ras , c fos or c jun protein . ^^^ Co injection of c myc , but not EJ ras , c fos or c jun , inhibits the ability of Rb to arrest the cell cycle . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
We examined the expression of the nuclear `` early response ' ' genes jun and fos and the `` tumor suppressor ' ' retinoblastoma ( Rb ) gene , as well as genes involved in invasion ( major excreted protein [ MEP ] , tissue inhibitor of metalloproteinases [ TIMP ] ) , and cell adhesion ( secreted phosphoprotein 1 [ SPP 1 ; also known as osteopontin ] ) . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
Here we report that Rb can repress c fos expression and AP 1 transcriptional activity in both serum induced and cycling 3T3 cells . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
The experiments reported herein were designed to examine the effects of either JANUS neutron or gamma ray exposure on expression of genes encoding nucleus associated proteins ( H 4 histone , c jun , c myc , Rb , and p 53 ) . ^^^ Results revealed induction of transcripts for c jun , H 4 histone , and ( to a lesser extent ) Rb following gamma ray but not following neutron exposure . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
Other downstream targets for ceramide action include Cox , IL 6 and IL 2 gene expression , PKC zeta , Vav , Rb , c Myc , c Fos , c Jun and other transcriptional regulators . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
We have examined by immunohistochemistry the c Fos and c Jun proteins in five cases of retinoblastoma in order to evaluate eventual alterations in their expression in vivo , possibly related to a gene mutation or to loss of Rb negative control . . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
We and others have previously shown that the SP family of mammalian transcription factors regulates the transcription of a number of genes whose activities are governed by the product of the retinoblastoma ( Rb ) susceptibility gene , including c FOS , c MYC , TGFbeta 1 , IGF 2 , and c JUN . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
Here , we report that GAL 4 Rb can repress transcription mediated by either Sp 1 , AP 1 , or p 53 , dependent upon the presence of both the GAL 4 DNA binding domain and GAL 4 binding sites . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
The present report summarizes the current molecular biological aspects of several oncogenes ( erbB , ras , myc , raf , fos , jun , bcl , mdm 2 , myb , kit CSF1R , met ) and tumor suppressor genes ( p 53 , rb , mts ) involved in lung cancer development with respect to the pathology of lung tumors , including the importance of these genes as far as the clinical course of the disease is concerned . . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
Here , we report that RB activates transcription of the c jun gene through the Sp 1 binding site within the c jun promoter . ^^^ A mechanism by which RB regulates transactivation is indicated by our detection of a heat labile and protease sensitive Sp 1 negative regulator ( s ) ( Sp 1 1 ) that specifically inhibits Sp 1 binding to a c jun Sp 1 site . ^^^ These findings provide evidence for a functional link between two distinct classes of oncoproteins , RB and c Jun , that are involved in the control of cell growth , and also define a novel mechanism for the regulation of c jun expression . . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
We also found that apoptotic mouse 3T3 fibroblasts express c fos , c myc , c jun , and cdc 2 , as well as the upregulation of RB phosphorylation and BrdU incorporation , just before final DNA fragmentation and death . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
Examination of the nucleotide sequence of the retinoblastoma ( Rb ) promoter revealed the presence of a DNA region highly homologous to the recognition site for the cellular transcription factor AP 1 . ^^^ A pair of complementary oligonucleotides containing the AP 1 site was synthesized and used in gel retardation assays to determine the role of the AP 1 protein in the regulation of the Rb gene expression . ^^^ These results suggest that AP 1 could be implicated in Rb gene transcriptional regulation through its interaction with the AP 1 binding site of the Rb gene promoter . . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
Immunoblot analyses were performed with specific antibody against BCL 2 , BCL XS / L , BAX , JUNB , c JUN , ICH 1L , c FOS , RB , CDK 2 , and p 53 . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
Transcription factor , SP 1 , and retinoblastoma susceptible gene product , RB , are substrates of SPase while other nuclear factors such as c Jun and c Fos are not . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
The promoter region of rhoB harbors regulatory elements such as TATA , CAAT , and Sp 1 boxes but not consensus sequences for AP 1 , Elk 1 , or c Jun / ATF 2 . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
But the transcriptional expression of c fos , c myc , c jun , H ras , 5 erb B oncogenes and Rb antioncogene in atherosclerotic lesion has not yet been reported . ^^^ The results showed that the atherosclerotic plaques contained 3 4 fold more 5 sis , c fos and c myc mRNA ( P < 0 . 01 ) , 2 fold more c jun and H ras mRNA ( P < 0 . 01 ) , and less Rb mRNA ( P < 0 . 05 ) than those in the normal aortic arteries . ^^^ These results indicate that the abnormal expression of 5 sis , c myc , c fos , c jun , and H ras oncogenes and Rb antioncogene may play an important role in arterial SMC proliferation and pathogenesis of atherosclerosis . . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
Growth arrest is accompanied by an increase in the levels of c jun , jun D , cyclin D 1 , C / EBP beta , transglutaminase type 2 , and retinoblastoma ( Rb ) mRNA and an induction of p 105 , the hypophosphorylated , growth regulatory form of Rb . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
Here we report that Rb binds to members of the AP 1 family of transcription factors , including c Jun , and stimulates c Jun transcriptional activity from an AP 1 consensus sequence . ^^^ Rb binds c Jun and activates transcription . ^^^ The interaction involves the leucine zipper region of c Jun and the B pocket of Rb as well as a C terminal domain . ^^^ The human papillomavirus type 16 E 7 protein , which binds to both c Jun and Rb , inhibits the ability of Rb to activate c Jun . ^^^ The results provide evidence of a role for Rb as a transcriptional activator in early G 1 and as a potential modulator of c Jun expression during keratinocyte differentiation . . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
Our data show that RB is proapoptotic in DU 145 cells and acts upstream of JUN expression and JNK activation . . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
Loss of 14D3 ter that encompasses the tumor suppressor gene Rb 1 in PARP ( / ) mice was associated with a reduction in retinoblastoma ( Rb ) expression ; increased expression of the oncogene Jun was correlated with a gain in 4C5 ter that harbors this oncogene . ^^^ These results further implicate PARP in the maintenance of genomic stability and suggest that altered expression of p 53 , Rb , and Jun , as well as undoubtedly many other proteins may be a result of genomic instability associated with PARP deficiency . . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
The methylation sensitive endonuclease SacII cuts twice within a key hairpin loop structure in the RB promoter that contains binding sites for AP 1 , Sp 1 and RBF 1 . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
Moreover , the hyperphosphorylated retinoblastoma protein ( Rb ) increased in c jun expressing cells , indicating that phosphorylated Rb may play a role in regulating Sp 1 to repress p 21 expression . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
Therefore , we studied protein expression of 6 cell cycle regulatory proteins ( Rb , p 16 , p 21 , p 27 , cyclin D 1 , and cyclin E ) in protein extracts from 53 breast cancer samples and 4 mammary cell lines and correlated the data with expression of the 7 AP 1 family members ( c jun , junB , junD , c fos , fosB , fra 1 , and fra 2 ) as determined in a previous study . ^^^ After Western blot analysis , we found significant associations between members of both groups : whereas c jun was associated with Rb expression ( p = 0 . 002 ) , strong junD and c fos expression correlated with high cyclin E reactivity ( p = 0 . 017 and p = 0 . 013 , respectively ) . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
In primary mouse embryo fibroblasts , activity of the minimal AP 1 dependent TF promoter was suppressed in Rb ( / ) cells compared to parallel Rb ( + / ) and Rb ( + / + ) transfectants . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
We previously showed that immortalized fibroblasts derived from PARP ( / ) mice exhibit an unstable tetraploid population , and partial chromosomal gains and losses in PARP ( / ) mice and immortalized fibroblasts are accompanied by changes in the expression of p 53 , Rb , and c Jun , as well as other proteins . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
Expression levels of the AP 1 factors were correlated with clinico pathologic tumor parameters , steroid receptor status , Ki 67 expression and the expression levels of eight cell cycle regulatory proteins ( cyclin D 1 , cyclin E , cyclin B 1 , cdk 2 , cdk 4 , p 16 , p 21 , and Rb ) . ^^^ On the other hand , correlations with classic cell cycle inhibitors ( Rb , p 16 , p 21 ) have also been observed for all AP 1 factors except c jun and junD . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
In addition , agents that affect Rb , such as E1A and E2F1 / DP 1 , when transfected into cells , also alter topo IIalpha Rb localization , activate jun kinase pathways and cause apoptosis . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
RhoB , not RhoA , represses the transcription of the transforming growth factor beta type 2 receptor by a mechanism involving activator protein 1 . ^^^ Deletion analysis of the T beta R 2 promoter identified a RhoB responsive region , and mutational analysis of this region revealed that a site for the transcription factor activator protein 1 ( AP 1 ) is critical for RhoB mediated repression of T beta R 2 transcription . ^^^ Electrophoretic mobility shift assays clearly showed that the binding of AP 1 to its DNA binding site is strongly inhibited by RhoB . ^^^ Consequently , transcription assays using an AP 1 reporter showed that AP 1 mediated transcription is down regulated by RhoB . ^^^ Altogether , these results identify a mechanism by which RhoB antagonizes TGF beta action through transcriptional down regulation of AP 1 in T beta R 2 promoter . . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
RESULTS : In contrast to non endocrine tumours of the digestive system such as carcinoma of the pancreas , colon and stomach , dNETs do not show alterations in oncogenes ( ras , Myc , fos jun , Src ) or in common tumor suppressor genes [ p 53 , retinoblastoma suspectibility gene ( Rb ) ] . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
These results suggest that merlin inhibits abnormal cell proliferation which is activated via Ras by repressing Rb phosphorylation , blocking the increase of the cyclin D 1 protein level , and inhibiting the activation of AP 1 and E2F 1 dependent transcription in NIH3T3 cells . . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
Activation of JUN N terminal kinase depended on the presence of RB and occurred within 8 h of irradiation . ^^^ ABL kinase , JUN N terminal kinase , and p 38 MAPK activity were all required for RB mediated DU 145 cell death after gamma irradiation . . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
Highly overexpressed genes include Th 2 cells specific transcription factors Gata 3 and Jun B , as well as integrin beta 1 , proteoglycan 2 , the RhoB oncogene , and dual specificity phosphatase 1 . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
Microarray based differential expression analysis revealed substantial ACVR 2 induced overexpression of genes implicated in the control of cell growth and tumorigenesis , including the activator protein ( AP ) 1 complex genes JUND , JUN , and FOSB , as well as the small GTPase signal transduction family members , RHOB , ARHE , and ARHGDIA . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
Downstream of PKC , PMA treatment inhibited extracellular signal regulated kinase mitogen activated protein kinase phosphorylation , up regulated c jun NH ( 2 ) terminal kinase phosphorylation , and inhibited retinoblastoma ( Rb ) phosphorylation . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
Mutation of cysteine 192 to glycine , alanine , or serine blocks the ability of RhoB to suppress transforming growth factor beta type 2 receptor , p2lwaf , and AP 1 promoter transcriptional activities . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
Detailed analysis of expression of selected genes in beta carotene treated LNCaP cells at the level of mRNA and protein indicated that the observed increase of proliferation could have been the result of slight induction of a few genes affecting proliferation ( c myc , c jun ) and apoptosis ( bcl 2 ) with no significant effect on major cell cycle control genes ( cdk 2 , RB , E2F 1 ) . . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
Potential mechanisms of growth inhibition , i . e . , regulation of the cell cycle control proteins E2F1 and retinoblastoma protein ( RB ) , and effect on activator protein 1 ( AP 1 ) mediated gene regulation were examined . beta apo 14 ' CA and atRA inhibited the expression of E2F1 protein in MCF 7 and Hs578T cells . beta apo 14 ' CA , beta apo 12 ' CA and atRA down regulated RB protein expression in MCF 7 but not in Hs578T cells . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
METHODS : The expression of various oncoproteins ( p 21 , EGFR 1 , erbB 2 , c jun , c myc , bcl 2 , Bax ) and suppressor gene products ( WT 1 , RB gene , p 53 ) was studied in normal urothelium , transitional cell carcinoma ( TCC ) cell lines and their MDR sublines by using specific antibodies and an indirect immunofluorescence flow cytometric method . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
A summary of what is known about some of these regulatory oncogenes ( fos , jun , myc , and Rb 1 ) and where they might function in the progression of a cell from G 0 to G 1 and G 1 to S is presented . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
Nine of these genes ( H ras , c myc , c fos , c jun , junB , N myc , c abl , c yes , and p 53 ) were expressed in epithelial cells , whereas two ( RB 1 and N ras ) were not . expression of four other genes ( c src , K ras , c raf , and c myb ) was observed , but the intensity of these bands was too low for densitometric analysis . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
The different genes involved in the BRCA 1 and BRCA 2 pathways ( GADD45A , BARD 1 , JUN , BAX , RB 1 , ERalpha , ERbeta , BAP 1 , TNFalpha , p 53 , p21Waf1 / Cip1 , p 300 , RAD 51 , pS 2 , Ki 67 ) were quantified by real time quantitative RT PCR , using the TaqMan method and an ABI Prism 7700 Sequence Detector ( Applied Biosystems ) . ^^^
Interacting proteins: P06400 and P05412 Pubmed SVM Score :0.0
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