| Interacting proteins: O75832 and P06400 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: O75832 and P06400 |
Pubmed |
SVM Score :0.0 |
| Gankyrin contains six ankyrin repeats and interacts with Rb , Cdk 4 , and the S 6 ATPase of the 26 S proteasome . ^^^ |
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| Interacting proteins: O75832 and P06400 |
Pubmed |
SVM Score :0.0 |
| Gankyrin interacts with the S 6 proteasomal ATPase and accelerates the degradation of the tumor suppressor Rb . ^^^ |
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| Interacting proteins: O75832 and P06400 |
Pubmed |
SVM Score :0.0 |
| Gankyrin bound to the product of the retinoblastoma gene ( RB 1 ) , increasing its phosphorylation and releasing the activity of the transcription factor E2F 1 . ^^^ Gankyrin accelerated the degradation of RB 1 in vitro and in vivo , and was identical to or interacted with a subunit of the 26S proteasome . ^^^ |
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| Interacting proteins: O75832 and P06400 |
Pubmed |
SVM Score :0.0 |
| Novel insights into the INK 4 CDK4 / 6 Rb pathway : counter action of gankyrin against INK 4 proteins regulates the CDK 4 mediated phosphorylation of Rb . ^^^ The newly discovered oncogenic protein gankyrin , which contains six ankyrin repeats , has been reported to be involved in the phosphorylation and degradation of the retinoblastoma gene product , Rb . ^^^ Using in vitro systems , we have identified a peptide fragment of gankyrin , 176LHLACDEERN185 , which is responsible for binding of gankyrin to Rb . ^^^ We further demonstrated a different mechanism for gankyrin to facilitate the phosphorylation of Rb , by binding with cyclin dependent kinase 4 ( CDK 4 ) . ^^^ We then showed that binding of gankyrin to CDK 4 and the consequent counter action of p 16 function were not affected by the Rb binding peptide 176LHLACDEERN185 , indicating that the two mechanisms are independent . ^^^ |
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| Interacting proteins: O75832 and P06400 |
Pubmed |
SVM Score :0.0 |
| RESULTS : In primary hepatocytes , gankyrin was expressed in the G1 / S phase of the cell cycle at 48 h after mitogenic stimulation , which coincided with the phase of cyclin D 1 RNA upregulation and RB 1 protein downregulation . ^^^ |
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| Interacting proteins: O75832 and P06400 |
Pubmed |
SVM Score :0.0 |
| The crystal structure of gankyrin , an oncoprotein found in complexes with cyclin dependent kinase 4 , a 19 S proteasomal ATPase regulator , and the tumor suppressors Rb and p 53 . ^^^ |
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| Interacting proteins: O75832 and P06400 |
Pubmed |
SVM Score :0.0 |
| Crystal structure of the homolog of the oncoprotein gankyrin , an interactor of Rb and CDK4 / 6 . ^^^ Gankyrin interacts with the retinoblastoma tumor suppressor ( Rb ) and cyclin dependent kinase 4 / 6 ( CDK4 / 6 ) , increases phosphorylation at specific residues of Rb by CDK4 / 6 in vivo , and promotes tumorigenesis . ^^^ The tertiary structure of Nas6p , together with the previous biochemical studies , indicates that the CDK4 / 6 and Rb binding surfaces of gankyrin are located at the N and C terminal regions , respectively , and face the same side of gankyrin . ^^^ These observations suggest that gankyrin brings Rb and CDK4 / 6 together through gankyrin Rb and gankyrin CDK4 / 6 interactions and determines the relative positioning of the substrate ( Rb ) and the enzyme ( CDK4 / 6 ) . ^^^ |
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| Interacting proteins: O75832 and P06400 |
Pubmed |
SVM Score :0.0 |
| Gankyrin : an intriguing name for a novel regulator of p 53 and RB . ^^^ Previous studies identified gankyrin as a component of the 26S proteasome that is consistently overexpressed in liver cancer and promotes cell transformation by binding RB . ^^^ These important findings implicate gankyrin as a dual purpose negative regulator of RB and p 53 , thereby identifying gankyrin as a rational cancer therapeutic target . . ^^^ |
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| Interacting proteins: O75832 and P06400 |
Pubmed |
SVM Score :0.0 |
| The oncoprotein gankyrin negatively regulates both p 53 and RB by enhancing proteasomal degradation . ^^^ Gankyrin also binds to CDK 4 and the tumor suppressor RB , and accelerates phosphorylation and proteasomal degradation of RB . ^^^ |
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| Interacting proteins: O75832 and P06400 |
Pubmed |
SVM Score :0.0 |
| Viral oncoprotein E 7 , Epstein Barr virus nuclear antigen 3C ( EBNA3C ) , human cytomegalovirus pp 71 and cellular oncoprotein gankyrin all contain the L 10 C 10 E Rb binding motif and target Rb protein for degradation in either ubiquitin dependent or ubiquitin independent proteasome pathways . ^^^ |
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