| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NMDA receptor subunits epsilon 1 ( NR2A ) and epsilon 2 ( NR2B ) are substrates for Fyn in the postsynaptic density fraction isolated from the rat brain . ^^^ |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Thus , whereas application of glutamate to hippocampal slices from control mice increased fyn protein tyrosine kinase ( PTK ) activity more than 2 . 5 fold , these changes were significantly impaired in LP BM 5 MuLV infected mice . ^^^ |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Glutamate activated currents ( evoked every 20 s for up to 20 min ) were increased by src and fyn kinases without affecting the desensitization and deactivation kinetics in NR 1 NR2A but the kinases had no effects in NR 1 NR2B , NR 1 NR2C and NR 1 NR2D receptor channels , suggesting that a phosphorylation site in NR2A is targeted . 4 . ^^^ |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Immunoblot analysis revealed that the relative amounts of metabotropic glutamate receptor 1alpha , N ethylmaleimide sensitive fusion protein , protein kinases C , Fyn and TrkB , as well as the neuronal nitric oxide synthase , were significantly higher in seizure developing than in control rats . ^^^ |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| We have investigated the tyrosine phosphorylation of NMDA receptor subunits NR2A and NR2B by exogenous Src and Fyn and compared this to phosphorylation by tyrosine kinases associated with the postsynaptic density ( PSD ) . ^^^ Src and Fyn phosphorylated similar sites in NR2A and NR2B , tryptic peptide mapping identifying seven and five major tyrosine phosphorylated peptides derived from NR2A and NR2B , respectively . ^^^ |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Tyrosine phosphorylation of ionotropic glutamate receptors by Fyn or Src differentially modulates their susceptibility to calpain and enhances their binding to spectrin and PSD 95 . ^^^ To test whether and how tyrosine phosphorylation of glutamate ionotropic receptor subunits modulates calpain susceptibility , synaptic membranes were phosphorylated by Fyn or Src , two members of the Src family tyrosine kinases . ^^^ Similar results were observed with NR2A and NR2B C terminal domain fusion proteins phosphorylated by Fyn or Src before incubation with calpain and calcium . ^^^ In addition , phosphorylation of NR2A and NR2B C terminal fusion proteins by Fyn or Src enhanced their binding to spectrin and PSD 95 . ^^^ |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| The SH 3 domain from the Fyn tyrosine kinase possesses a buried hydrogen bond between the side chains of a glutamate ( Glu 24 ) and a serine ( Ser 41 ) residue . ^^^ |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| The ischemia induced increase in the interaction of NR2A and NR2B with the SH 2 domains of Src and Fyn suggests a possible mechanism for the recruitment of signaling proteins to the PSD and may contribute to altered signal transduction in the postischemic hippocampus . . ^^^ |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Non receptor type tyrosine kinase Fyn in the Src family has been suggested to promote kindling development via tyrosine phosphorylation of the NMDA receptor subunit , NR2B . ^^^ |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Our data suggest that increased expression of genes encoding the following proteins are involved in oligodendroglial differentiation : Fyn , Erk , p 85 , G alpha 12 guanine nucleotide binding , and transducin beta 2 signal transduction molecules ; glial maturation factor ; the proteasomal subunits C 8 and C 3 ; the proteasomal targeting molecule polyubiquitin ; the cell cycle regulatory proteins Set , protein phosphatase 2A , and nuclear tyrosine phosphatase ( PRL 1 ) ; and the high affinity glutamate cotransporter EAAC 1 . . ^^^ |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Characterization of Fyn mediated tyrosine phosphorylation sites on GluR epsilon 2 ( NR2B ) subunit of the N methyl D aspartate receptor . ^^^ To precisely address the roles of NMDA receptor tyrosine phosphorylation , we identified Fyn mediated phosphorylation sites on the GluR epsilon 2 ( NR2B ) subunit of NMDA receptors . ^^^ |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| We found that RACK 1 binds both the NR2B subunit of the NMDA receptor and the nonreceptor protein tyrosine kinase , Fyn . ^^^ RACK 1 inhibits Fyn phosphorylation of NR2B and decreases NMDA receptor mediated currents in CA 1 hippocampal slices . ^^^ Peptides that disrupt the interactions between RACK 1 , NR2B , and Fyn induce phosphorylation and potentiate NMDA receptor mediated currents . ^^^ |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| This apparent lack of alcohol withdrawal induced behavioural effects was associated with increased Fyn activity and tyrosine phosphorylation of several proteins including the NMDA receptor subunit NR2B in the different mutant lines . ^^^ NR2B phosphorylation itself remained unaffected by the chronic alcohol ingestion and subsequent withdrawal , but challenge with an NR2B antagonist , ifenprodil , restored a normal behavioural response in alcohol withdrawn fyn mutants . ^^^ Together , these results suggest that Fyn can modulate alcohol consumption and prevent behavioural changes during alcohol withdrawal , possibly via phosphorylation of NR2B . . ^^^ |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| We have shown that an Src family kinase Fyn phosphorylates NR2A and NR2B subunits of the NMDA receptor . ^^^ A phosphorylation of one of the sites on NR2B ( Tyr 1472 ) is largely dependent on Fyn and is elevated upon the LTP induction of hippocampal CA 1 neurons . ^^^ |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| We found that RACK 1 binds both the NR2B subunit of the NMDA receptor and the nonreceptor protein tyrosine kinase , Fyn . ^^^ RACK 1 inhibits Fyn phosphorylation of NR2B and decreases NMDA receptor mediated currents in CA 1 hippocampal slices ( Yaka , R . , Thornton , C . , Vagts , A . ^^^ We found that activation of the cAMP / protein kinase A pathway in hippocampal slices induced the release of RACK 1 from NR2B and Fyn . ^^^ This resulted in the induction of NR2B phosphorylation and the enhancement of NMDA receptor mediated activity via Fyn . ^^^ |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Spatial learning induced phosphorylation of TrkB , Fyn , and NR2B , but not NR2A , in the hippocampus . ^^^ Fyn was coimmunoprecipitated with TrkB and NR2B , and this association was increased in well trained rats compared with control animals . ^^^ The phosphorylation of Fyn and NR2B , but not TrkB , was diminished by PP 2 treatment . ^^^ |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| A Src family tyrosine kinase , Fyn plays a role in this interaction by linking TrkB with NR2B . ^^^ |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| We demonstrate that , in the hippocampus but not in the cerebral cortex , Fyn is targeted to the NR2B subunit of the NMDA receptor by the scaffolding protein RACK 1 . ^^^ During acute exposure to ethanol , RACK 1 is dissociated from the complex , thereby facilitating Fyn mediated phosphorylation of NR2B , which enhances channel activity , counteracting the inhibitory actions of ethanol . ^^^ |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Dopamine D 1 dependent trafficking of striatal N methyl D aspartate glutamate receptors requires Fyn protein tyrosine kinase but not DARPP 32 . ^^^ In the Fyn knockout mice , basal tyrosine phosphorylation of NR2A and NR2B was drastically reduced , whereas serine phosphorylation of these NMDA subunits was unchanged . ^^^ |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| The interaction of BDNF / TrkB signaling with N methyl D aspartate receptors is important for spatial learning and memory , and an Src family tyrosine kinase Fyn may play a key role in this interaction by linking TrkB with NR2B . . ^^^ |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| We previously demonstrated that transgenic mice overexpressing Fyn tyrosine kinase exhibit higher seizure susceptibility and enhanced tyrosine phosphorylation of several proteins , including the N methyl D aspartate ( NMDA ) receptor subunit 2B ( NR2B ) . ^^^ However , the selective NR2B antagonist ifenprodil , when administered before conditioning , restored tone dependent freezing in Fyn TG mice at a dose that did not affect freezing in wild type ( WT ) mice . ^^^ These results suggest that impairment of tone dependent conditioned freezing in Fyn TG mice is caused by disruption of the NR2B containing NMDA receptor function . ^^^ Tyrosine phosphorylation of brain proteins , including NR2B , was enhanced in Fyn TG mice compared with that in WT mice . ^^^ |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| This study identifies a dynamic regulation of synaptic NR2B containing NMDARs through PDZ protein mediated stabilization and AP 2 mediated internalization that is modulated by phosphorylation by Fyn kinase . . ^^^ |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Fyn kinase and NR2B containing NMDA receptors regulate acute ethanol sensitivity but not ethanol intake or conditioned reward . ^^^ Recently , we found that the compartmentalization of Fyn to the NR2B subunit of the NMDA receptor ( NMDAR ) in the hippocampus regulates Fyn phosphorylation of NR2B in response to ethanol , which mediates the acute tolerance of NMDAR to ethanol inhibition in hippocampal slices . ^^^ In this study we determined , first , whether acute tolerance to ethanol inhibition is mediated via NR2B containing NMDARs in vivo and , second , whether the increase in acute sensitivity to ethanol in the Fyn / mice influences ethanol consumption or ethanol ' s conditioned rewarding effects . ^^^ RESULTS : We found that systemic injection of the NR2B containing NMDAR selective antagonist , ifenprodil , abolished the differences between Fyn+ / + and Fyn / mice in sensitivity to the acute sedative effects of ethanol . ^^^ CONCLUSIONS : Our results suggest that the interaction between Fyn and NR2B mediates the acute sedative effects of ethanol , and that alteration in acute ethanol sensitivity does not necessarily correlate with levels of ethanol consumption or the rewarding properties of ethanol . . ^^^ |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Fyn kinase mediated phosphorylation of NMDA receptor NR2B subunit at Tyr 1472 is essential for maintenance of neuropathic pain . ^^^ Neuropathic pain and NR2B phosphorylation at Tyr 1472 were attenuated by the NR2B selective antagonist CP 101 , 606 and disappeared in mice lacking Fyn kinase , a Src family tyrosine kinase . ^^^ The present study demonstrates that Tyr 1472 phosphorylation of NR2B subunits by Fyn kinase may have dual roles in the retention of NMDA receptors in the postsynaptic density and in activation of nitric oxide synthase , and suggests that PGE 2 is involved in the maintenance of neuropathic pain via the EP 1 subtype . . ^^^ |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Fyn activation and enhanced tyrosine phosphorylation of the NMDA receptor NR2B subunit , as measured by Western blotting , were induced after haloperidol injection of the control mice , but both responses were significantly reduced in Fyn deficient mice . ^^^ Dopamine D ( 2 ) receptor blockade was shown to increase both NR2B phosphorylation and the NMDA induced calcium responses in control cultured striatal neurons but not in Fyn deficient neurons . ^^^ Based on these findings , we proposed a new molecular mechanism underlying haloperidol induced catalepsy , in which the dopamine D ( 2 ) receptor antagonist induces striatal Fyn activation and the subsequent tyrosine phosphorylation of NR2B alters striatal neuronal activity , thereby inducing the behavioral changes that are manifested as a cataleptic response . . ^^^ |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| We also found that the increase in NR2A and NR2B proteins in heterozygous reeler was dependent on Fyn , because this change was absent in heterozygous reeler / homozygous Fyn deficient double mutant mice . ^^^ |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| In the first presentation , Dorit Ron presented data showing how activation of Fyn or Src tyrosine kinases differentially regulated the cell surface expression and activity of NR2A and NR2B containing NMDA receptors . ^^^ |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| In a human embryonic kidney ( HEK ) 293 cell expression system , PTPalpha enhanced fyn mediated NR2A and NR2B tyrosine phosphorylation by several fold . ^^^ |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06241 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
|