| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.66598053 |
| By low stringency screening of a Xenopus laevis oocyte cDNA library with a probe prepared from the yes oncogene of the avian retrovirus Y 73 , we have isolated clones encoding a 537 amino acid protein with 96 % amino acid identity to the protein product of the human fyn proto oncogene . 0.66598053^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Nonreceptor PTK encoded by mammalian proto oncogenes ( c src , fyn , c yes , c abl ) and their homologs in Drosophila exhibit patterns of expression and activities that suggest functions in axonal or dendritic outgrowth . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| It is clear , however , that c src is the prototype of a family of at least 8 closely related genes encoding protein tyrosine kinases , the other family members being blk , c fgr , fyn , hck , lck , lyn , and c yes . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Conservation of structure and expression of the c yes and fyn genes in lower vertebrates . ^^^ We have cloned the c yes and fyn homologues of the src family from the teleost fish Xiphophorus helleri . ^^^ In malignant melanoma , elevated levels of c yes and fyn were detected indicating a possible function during secondary steps of tumor progression for src related tyrosine kinases . . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| To date the src gene family consists of at least 9 closely related protein tyrosine kinases belonging to the non receptor type of kinases : c src , c yes , c fgr , fyn , lyn , lck , hck , tkl and bkl . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| The enzyme reacted quantitatively with a monoclonal antibody to the amino terminal sequence ( Cys 3 to Ser 66 ) specific to the human c yes protein , but not with antibodies to the specific amino terminal sequences of the c src , fyn , and lck proteins . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| The C SRC , C YES , and FYN genes encode three closely related tyrosine protein kinases that are expressed in human neural tissues . ^^^ The C YES and FYN genes share with C SRC similar exon intron boundaries and a high degree of amino acid sequence homology in the 3 / 4 exon coding region . ^^^ However , potential alternative splicing of the C YES and FYN genes in this region has not been previously investigated . ^^^ In this study we have compared the expression of C SRC , C YES , and FYN RNAs in human lung , liver , brain , and placenta tissues and prepared cDNA clones spanning exons 3 and 4 for each of these genes from the different tissues . ^^^ Sequence analysis of these cDNA clones revealed that the splicing patterns for the FYN and C YES genes were the same among the various tissues , whereas C SRC cDNAs isolated from brain contained 18 additional bases with the capacity to code for the same six amino acids present in the neural specific forms of avian and murine pp60c src . . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Sequence similarity with human c src , c yes , and fyn translational products began at amino acid position 76 of the predicted c fgr protein and extended nearly to its C terminus . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| By screening both human genomic and cDNA libraries using 5 yes DNA as a probe , we obtained DNA clones of the c yes gene , the pseudogene of c yes , c fgr gene and c src gene and two novel yes related genes , fyn and lyn . ^^^ Complete nucleotide sequence analysis of the cDNA clones of c yes , fyn and lyn revealed that these genes encode proteins similar to p60src both in size and sequence . . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| To explore whether other members of the c Src family are activated in these mammary tumors , we measured the in vitro kinase activity of the c Yes and Fyn kinases in protein extracts derived from mammary tumor tissue and morphological normal adjacent tissue . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| The potent transforming activity of PyV middle T antigen can , in part , be attributed to its ability to associate with and to activate a number of c Src family tyrosine kinases ( c Src , c Yes , and Fyn ) . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| The c src gene family has nine known members ( blk , c fgr , fyn , hck , lck , lyn , c src , c yes and yrk ) , each encoding a cytoplasmic protein tyrosine kinase ( PTK ) believed to be involved in signal transduction . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Middle T antigen of mouse polyomavirus , an oncogenic DNA virus , associates with and activates the cellular tyrosine kinases c Src , c Yes , and Fyn . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Specific antibodies against c Src , Fyn , c Yes , c Fgr , Lck , Lyn , Hck , and Blk proteins did not recognize the p 57 Xenopus tyrosine kinase . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| In NIH3T3 fibroblasts , c Src , Fyn , and c Yes associate with the activated PDGF receptor , are substrates for receptor phosphorylation , and are themselves activated . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Middle T antigen of mouse polyomavirus ( MomT ) associates with the cellular tyrosine kinases c Src , c Yes , and Fyn , while middle T antigen of hamster polyomavirus ( HamT ) exclusively binds Fyn . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Endothelin ( ET ) , a well known hypertrophic agonist , increased activity of c Src , c Yes , and Fyn within minutes and promoted a selective redistribution of each of these kinases within the cell . ^^^ Overexpression of c Src effected a significant increase in activity of a cotransfected human ANP promoter driven chloramphenicol acetyl transferase reporter , while expression of either c Yes or Fyn was considerably less effective in this regard . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Like E and P cadherin , Ca ( 2+ ) treatment of normal and tumor derived human keratinocytes resulted in c Yes ( and c Src and Fyn ) , as well as their putative substrate p 120 ( CTN ) , being recruited to cell cell contacts . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| A 2 . 8 kilobase transcript of the c syn gene , which differs in size from those of the c yes , c src , and c fgr genes , was observed in various cell types . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Moreover , by using the specific platelet derived growth factor receptor ( PDGFR ) inhibitor AG 1296 or by overexpressing a kinase mutant PDGFR , we show that PDGFR kinase activity is essential for 5 HT2B triggered MAPK / cyclin D 1 , but not cyclin E , signaling pathways . 5 HT2B receptor activation also increases activity of the Src family kinase , c Src , Fyn , and c Yes . ^^^ Strikingly , c Src , but not Fyn or c Yes , is the crucial molecule between the G ( q ) protein coupled 5 HT2B receptor and the cell cycle regulators . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Both null cells for pp 60 ( c src ) and triple knockout cells for pp 60 ( c src ) , pp 59 ( fyn ) , and pp 62 ( c yes ) exhibited decreased phosphotyrosine levels in focal contacts when compared with wild type cells . pp 60 ( c src ) null cells also exhibited faster assembly of cell matrix adhesions and a more exuberant recruitment of FAK to these sites . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| The Src family kinases c Src , c Yes , Fyn , and Lyn were expressed and activated by transforming growth factor alpha stimulation in all four SCCHN cell lines examined but not in corresponding normal epithelial cells . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Notably , alpha 4 stimulated cell motility was inhibited by catalytically inactive receptor protein tyrosine phosphatase alpha overexpression and blocked by the p50Csk phosphorylation of c Src at Tyr 529 . alpha4beta1 stimulated cell motility of triple null Src ( / ) , c Yes ( / ) , and Fyn ( / ) fibroblasts was dependent on c Src reexpression that resulted in p130Cas tyrosine phosphorylation and Rac GTPase loading . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Three members of the Src family of tyrosine kinases [ pp60c src ( Src ) , p59fyn ( Fyn ) and pp62c yes ( Yes ) ] are ubiquitously expressed , and are thus likely to have general roles in growth control . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| GAP association with Fyn , Lyn , and Yes in thrombin activated platelets . ^^^ We show that GAP and two proteins of 59 and 68 kDa are phosphorylated on tyrosine after thrombin stimulation and that three Src related protein tyrosine kinases , Fyn , Lyn and Yes , are associated with GAP in complexes , detectable only after agonist stimulation . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| The fyn , fgr , and lyn genes were characteristically expressed concomitantly with phenotypic changes and expressions of nuclear proto oncogenes , whereas src , lck , hck , and yes genes were not . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| In this study we use digitonin to solubilize a murine T cell hybridoma and demonstrate that antibodies binding extracellular but not intracellular domains of the TCR specifically coprecipitate only the fyn PTK and not lck or yes , two other kinases found in these cells . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Recently , two new genes , syn and lyn , were found to encode proteins closely related to the yes product . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| The pattern of lyn mRNA expression was different from those of related genes , such as yes and syn . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Thus , src , fyn and yes are activated upon stimulation by platelet derived growth factor or colony stimulating factor in cells expressing high levels of these receptors . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| We report here that the complexes prepared from N2a cells display the large size and low buoyant density characteristic of complexes isolated from sources that are rich in caveolae , and contain the same major constituents , including multiple GPI anchored proteins , alpha and beta subunits of heterotrimeric G proteins , and the tyrosine kinases fyn and yes . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Using the anti Src antibody , which is reactive to Src , Fyn and Yes , we obtained evidence for an association between the Src family tyrosine kinase and the tyrosine phosphorylated Sky receptor . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Hypoxia increases the kinase activity of pp60c src and its phosphorylation on tyrosine 416 but does not activate Fyn or Yes . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Fyn and Yes , the other members of the Src family present in fibroblasts , were also found to be activated at mitosis . ^^^ In cells microinjected with a neutralizing antibody specific for Src , Fyn , and Yes ( anti cst . 1 ) during G 2 , cell division was inhibited by 75 percent . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| We show that CSF 1 stimulation resulted in activation of three Src family kinases , Src , Fyn and Yes . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Protein tyrosine kinases encoded by the fyn and yes proto oncogenes were expressed in Mller glia enriched cultures , and could contribute to the observed protein tyrosine phosphorylation . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| This up regulation is specific for p56lck since expression of other src kinases such as fyn , lyn , or yes was not modified . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Two other nonreceptor tyrosine kinases , YES and FYN , were concentrated in the GCPs to a similar degree as that seen for SRC . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Fyn immunoprecipitates displayed fourfold lower levels of middle T phosphorylation than that detected in the Src and Yes immunoprecipitates . ^^^ In Src negative cells , the level of middle T phosphorylation in Yes and Fyn immunoprecipitates was not significantly different from that detected in the Src positive cells , suggesting that the absence of Src does not lead to a compensating increase in the proportion of middle T associated with these kinases . ^^^ Taken together , these results indicate that Src is not required for middle T induced hemangiomas in mice or for focus induction in immortalized fibroblasts , and that the residual level of Yes , Fyn and phosphatidylinositol kinase activity associated with middle T in Src negative cells may compensate for the absence of Src . . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| To examine the role of Src related proteins in human colon carcinoma we measured the tyrosine kinase activity of pp60c src ( Src ) , p62c yes ( Yes ) , p56lck ( Lck ) , p59fyn ( Fyn ) , p59hck ( Hck ) , p56lyn ( Lyn ) and p55c fgr ( Fgr ) from colonic cells . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| We have examined interactions between FGF receptor 1 ( FGFR 1 ) and Src , Fyn and Yes . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| An antibody specific for the carboxy terminus of Src , Fyn , and Yes ( anti cst . 1 ) inhibited Src kinase activity in vitro and caused morphological reversion of Src transformed cells in vivo . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| This distribution pattern is similar to that of the Src family kinases including Yes , Src , Fyn and Lyn . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Members of the Src family such as Fyn and Yes showed an increased level of phosphorylation upon VEGF stimulation of cells expressing Flt 1 but not in cells expressing KDR . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| This type of analysis has only recently become possible , with the generation of ' gene knock out ' mice lacking each of the kinase genes src , fyn and yes . ^^^ RESULTS : Hemangiomas develop efficiently in newborn mice lacking either src , fyn or yes after inoculation with a PymT transducing retrovirus . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Combined deficiencies of Src , Fyn , and Yes tyrosine kinases in mutant mice . ^^^ Three members of the Src family of tyrosine kinases , src , fyn , and yes , are broadly expressed throughout mouse development . ^^^ Whereas most of the src / fyn or src / yes double mutants die perinatally , a substantial proportion of fyn / yes double mutants are viable but undergo degenerative renal changes leading to diffuse segmental glomerulosclerosis . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| We have investigated whether the Src , Fyn , Yes and Lck gene products are palmitoylated . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Most of the nine members of the Src family of tyrosine kinases are restricted in their expression , often to cells of the haematopoietic lineage , while some , particularly Src , Fyn and Yes , are more ubiquitously expressed . ^^^ We have been studying the functions of Src , Fyn and Yes in fibroblasts . ^^^ We have shown that stimulation of quiescent fibroblasts with platelet derived growth factor ( PDGF ) causes Src , Fyn and Yes to become activated , and to associate transiently with the PDGF receptor . ^^^ To address the role of Src , Fyn and Yes in the response to PDGF , we have used a dominant negative approach , in which cells were engineered to express catalytically inactive forms of Src kinases . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| One of the kinases was identified as the Torpedo homolog of neuronal fyn , whereas the other was a novel kinase we have named fyk due to its homology to both fyn and yes protein tyrosine kinases . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Non receptor type tyrosine kinases of the Src family , such as Src , Yes and Fyn , are strongly expressed in the brain and have been suggested to have an important function in the central nervous system . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Expression of various oncogenes ( ras , myc , erbB 2 , src , fyn , yes and sis ) in a high metastatic clone ( MH 02 ) derived from a murine methylcholanthrene induced fibrosarcoma A ( Meth A ) was compared with those of its parent clone ( ML 01 ) by Northern blot analysis . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Three members of the Src family of protein tyrosine kinases Src , Fyn , and Yes associate with the activated platelet derived growth factor ( PDGF ) receptor in vivo . ^^^ Injection of an antibody specific for Src , Fyn , and Yes also reduced signal transduction through the PDGF receptor but only when injected within 8 hr of PDGF stimulation . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| The sequence of the Yrk protein showed 79 % identity with human Fyn and 72 % identity with chicken Yes . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| No binding occurs to the related kinases Fyn or Yes or to the corresponding regions from the gamma and delta subunits . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Src and Src family members are reported to be membrane associated , but detergent extraction studies have demonstrated a major difference in the solubility of Src compared with other members of the Src family ( Fgr , Fyn , Lck , Lyn , and Yes ) , suggesting that their subcellular distributions may be different . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Here we show that while only Src and Yes were expressed and activated by EGF , microinjected kinase inactive mutants of Src ( SrcK ) and Fyn ( FynK ) were able to exert a dominant negative effect on the scattering response . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Like Fyn , Lyn , and Yes , most of Fgr and Lck was detergent insoluble in human and rat platelets . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Moreover , two Src family kinases ( Fyn and Yes ) were activated by angiotensin 2 in a time and concentration dependent manner . ^^^ Microinjection of antibodies against Fyn and Yes blocked angiotensin 2 induced DNA synthesis and c Fos expression in WB cells , indicating an obligatory involvement of these tyrosine kinases in the activation of the ERK cascade by angiotensin 2 . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Fyn , Yes , and Syk phosphorylation sites in c Cbl map to the same tyrosine residues that become phosphorylated in activated T cells . ^^^ Coexpression experiments in COS cells demonstrate that among T cell expressed Src and Syk related protein tyrosine kinases , Fyn , Yes , and Syk appear to play a major role in phosphorylation of c Cbl , whereas Lck and Zap phosphorylate c Cbl ineffectively . ^^^ Fyn , Yes , and Syk phosphorylate the same sites of c Cbl that become phosphorylated in stimulated T cells . ^^^ Among these kinases , Fyn and Yes demonstrate strong binding to c Cbl , which involves both phosphotyrosine dependent and phosphotyrosine independent mechanisms . . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Using coprecipitation methods with convulxin coupled beads and antibodies against FcRgamma and the Src family , we showed that Fyn and Lyn , but not Yes , Src , Fgr , Hck , and Lck , were physically associated with the GPVI FcRgamma complex irrespective of stimulation . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Western blot analysis with specific antibodies against Src family members showed expression of Src , Fyn , and Lyn , lower levels of Yes and Hck , and the absence of Lck tyrosine kinase . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| We have generated mouse embryos harboring functional null mutations of the ubiquitously expressed SFKs Src , Yes and Fyn . ^^^ To elucidate the molecular mechanisms underlying this phenotype , SYF cells ( deficient for Src , Yes and Fyn ) were derived and tested for their ability to respond to growth factors or plating on extracellular matrix . ^^^ Our studies reveal that while Src , Yes and Fyn are largely dispensable for platelet derived growth factor ( PDGF ) induced signaling , they are absolutely required to mediate specific functions regulated by extracellular matrix proteins . ^^^ Fibronectin induced tyrosine phosphorylation of focal adhesion proteins , including the focal adhesion kinase FAK , was nearly eliminated in the absence of Src , Yes and Fyn . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Binding analyses indicated a direct interaction of Tio with the SH 3 domains of Lyn , Hck , Lck , Src , Fyn , and Yes . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| We identified 30 tyrosine kinases expressed in these tissues : they include 10 non receptor tyrosine kinases ( yes , fyn , lyn , brk , abl , arg , jak 1 , jak 3 , tyk 2 and itk ) , 17 receptor tyrosine kinases ( erbB 2 , PDGF Ralpha , PDGF Rbeta , kit , c fms , met , ron , FGF R 1 , FGF R 2 , FGF R 3 , FGF R 4 , cek 5 , tie 1 , tkt , axl , sky and Ins R ) , 2 dual kinases ( mek and sek ) and one possible novel kinase . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Our data demonstrate that the tyrosine kinases p 56 ( Hck ) , p 72 ( Syk ) , p 77 ( Btk ) , p 62 ( Yes ) , p 56 ( Lck ) , and p 59 ( Fyn ) were specifically activated in response to spores from A . candidus , whereas spores from either A . niger or E . amstelodami activated p 56 ( Hck ) , p 72 ( Syk ) , and p 77 ( Btk ) . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Western blot analysis identified the src like protein tyrosine kinases p 53 ( lyn ) , p 56 ( lyn ) , p56 / 59 ( hck ) , p 55 ( fgr ) , and p 56 ( lck ) whereas p 60 ( src ) , p 62 ( yes ) , p 55 ( blk ) , and p 59 ( fyn ) were not detected . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| A growing body of literature suggests that the ubiquitously expressed Src family kinases ( Src , Fyn and Yes ) are required for agents such as platelet derived growth factor ( PDGF ) to stimulate DNA synthesis . ^^^ Yet Klinghoffer and colleagues recently presented evidence that fibroblasts derived from mice null for Src , Fyn and Yes responded normally to PDGF ( Klinghoffer et al . , 1999 , EMBO J . , 18 : 2459 2471 ) . ^^^ Furthermore , injection of SrcK or the cst . 1 antibody ( which inhibits Src , Fyn and Yes ) failed to inhibit PDGF stimulated DNA synthesis in NIH3T3 cells expressing dominant negative p 53 , and fibroblasts derived from p 53 null embryos . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| In particular , p60Src , but not the other ubiquitous SFKs , Fyn and Yes , was responsible for the observed effects . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Src family kinase expression was measured in 52 human mammary tumor ( T ) specimens compared with non tumor ( NT ) tissue from the same patient by enzymatic assays employing a Src kinase family specific peptide substrate and by immunoblotting with an antibody recognizing the Src family kinases Src , Fyn , and Yes . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| The Src kinases , Src , Yes and Fyn , enhance the efficiency of GAS uptake but are not absolutely required for GAS entry . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Coimmunoprecipitation experiments indicate that Fyn and , to a lesser extent , Yes combine with alpha6beta4 . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| In this report we show that both wild type and hck ( / ) fgr ( / ) macrophages expressed Fyn , Src , and Yes and that these kinases were activated on ingestion of immunoglobulin G ( IgG ) coated particles and redistributed , together with Syk , to actin rich phagocytic cups and the phagosomal membrane . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| RT PCR studies demonstrate expression of multiple Src tyrosine kinases , including Src , Fyn , and Yes , in CSMC . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Loss of function microinjection studies indicate that the ubiquitous Src , Fyn , and Yes tyrosine kinases are specifically implicated in Xenopus gastrulation movements . ^^^ Injection of mRNAs coding for dominant negative forms of the ubiquitous members of the Src family , namely Fyn , Src , and Yes , perturbs gastrulation movements , resulting in the inability to close the blastopore . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| L26A E 5 does not transform SYF cells that are deficient for Src , Fyn and Yes , unless Src expression is reconstituted , and does not transform NIH3T3 cells in which Src PTK activity is maintained at a basal level by means of kinase defective K295R Src overexpression . . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Src , Fyn , and Yes are not required for neuromuscular synapse formation but are necessary for stabilization of agrin induced clusters of acetylcholine receptors . ^^^ Mice deficient in src and fyn or src and yes move and breathe poorly and die perinatally , consistent with defects in neuromuscular function . ^^^ Src and Fyn are associated with acetylcholine receptors ( AChRs ) in muscle cells , and Src and Yes can act downstream of ErbB 2 , suggesting roles for Src family kinases in signaling pathways regulating neuromuscular synapse formation . ^^^ We studied neuromuscular synapses in src ( / ) ; fyn ( / ) and src ( / ) ; yes ( / ) mutant mice and found that muscle development , motor axon pathfinding , clustering of postsynaptic proteins , and synapse specific transcription are normal in these double mutants , showing that these pairs of kinases are not required for early steps in synapse formation . ^^^ Another Src family member , most likely Yes , was associated with AChRs and phosphorylated by agrin in myotubes lacking Src and Fyn , indicating that Yes may compensate for the loss of Src and Fyn . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Fluorescence spectroscopy shows that Tip binds to the SH 3 domains of several Src kinases ( Lck , Hck , Lyn , Src , Fyn , Yes ) , exhibiting the highest affinities for Lyn , Hck , and Lck . . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| In the corresponding murine fibroblast cell line SYF , which lacks the ubiquitously expressed SRC family kinases SRC , YES , and FYN , forskolin barely stimulated MAP kinase ; this reduction was reversed in cells in which c SRC had been reintroduced . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Estrogen stimulation of murine embryonic fibroblasts with homozygous deletions of the c src , fyn , and yes genes failed to induce Akt phosphorylation , whereas cells maintaining c Src expression demonstrated estrogen induced Akt activation . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| However , Fyn and Yes , unlike Src , were found to associate preferentially with Flt 1 . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Protein levels of Src family kinases such as Src , Fyn , and Yes were unchanged by lithium treatment . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Yes tyrosine kinase also binds to p 120 catenin but only upon activation , and stimulates Fer and Fyn tyrosine kinases . ^^^ These results indicate that p 120 catenin acts as a docking protein facilitating the activation of Fer / Fyn tyrosine kinases by Yes and demonstrate the role of these p 120 catenin associated kinases in the regulation of beta catenin alpha catenin interaction . . ^^^ |
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| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Nephrin fractionated in detergent resistant glomerular membrane fractions with Fyn and Yes . ^^^ Glomeruli in which Fyn , Yes , or Fyn and Yes were genetically deleted in mice were characterized to explore the relationship between these kinases and Nephrin . ^^^ Similar to Fyn deletion , simultaneous deletion of Fyn and Yes reduced Nephrin phosphorylating activity . ^^^ |
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| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| SYF cells , which lack expression of SFKs Src , Yes , and Fyn , were used to determine the role of SFKs in IL 6 family cytokine signaling and gene induction . ^^^ |
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| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Using fibroblasts deficient in Src , Yes and Fyn , we were able to show that there are multiple kinases present in the cell that can phosphorylate LRP 1 . ^^^ |
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| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Stress induced caveolin phosphorylation was abolished by three Src kinase inhibitors , SU 6656 , PP 2 and PD 180970 , and was not observed in fibroblasts derived from a Src , Yes and Fyn triple knockout mouse ( SYF / ) . ^^^ Using cell lines derived from single kinase knockout mice ( Src / , Yes / and Fyn / ) , we show that expression of Fyn , but not Src or Yes , is required for stress induced caveolin phosphorylation . ^^^ |
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| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Five members of the Src family of non receptor protein tyrosine kinases Lck , Lyn , Fyn , Src , and Yes are known to be expressed in the central nervous system . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Here we show that the Src family kinases Yes and Lyn , but not Src and Fyn , are expressed in isolated pancreatic acini of Wistar rats . ^^^ |
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| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Array profiles demonstrate that Fyn , Lyn , Fgr , Hck , Src , and Yes are all expressed in cultured human MKs ( Fyn , Lyn > Src , Yes , Fgr , Hck ) . ^^^ Similarly , Western blots of murine MKs identified the same six SFKs ( Fyn , Fgr , Hck , Lyn , Src , and Yes ) . ^^^ |
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| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Protein tyrosine phosphatase epsilon activates Yes and Fyn in Neu induced mammary tumor cells . ^^^ We show here that RPTP is a physiological activator of two additional Src family kinases , Yes and Fyn . ^^^ In agreement , opposite effects on activities and phosphorylation of Yes and Fyn are observed following increased expression of PTP . ^^^ Surprisingly , expression of Yes or of Fyn does not rescue the morphological phenotype of RPTP deficient tumor cells in contrast with the strong ability of Src to do so . ^^^ We conclude that RPTP activates Src , Yes , and Fyn , but that these related kinases play distinct roles in Neu induced mammary tumor cells . . ^^^ |
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| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Hyperosmotic exposure ( 405 mosm ) resulted in a rapid activation of the Src kinase family members Yes , Fyn , and Lck . ^^^ Hyperosmotic Yes , but not Fyn activation , was antioxidant sensitive and was followed by a rapid Yes / EGFR association . ^^^ PP 2 abolished the hyperosmotic activation of Fyn and Lck but not activation of Yes and EGFR and their association . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Mouse embryo fibroblasts ( MEFs ) deficient for src , yes and fyn , and those lacking all p38MAPK isoforms displayed normal Aplidin sensitivity ( IC50=12 nM ) . ^^^ |
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| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| In addition , we found this phosphorylation to be independent of the Src family kinases Src , Fyn and Yes . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Immunoprecipitation of the alpha2beta1 integrin in unstimulated SMCs followed by immunoblotting for the PDGFRbeta as well as Src family members , pp 60 ( src ) , Fyn , Lyn , and Yes demonstrated coassociation of alpha2beta1 and the PDGFRbeta as well as pp 60 ( src ) . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| These results suggest that CK 2 may be a protein kinase responsible for the suppression of at least three Src TKs ( Fyn , Src and Yes ) through the specific phosphorylation of their Thr residues at the cellular level . . ^^^ |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Induction of SM22alpha by TGF beta 1 was markedly attenuated in SYF cells ( c Src ( ) , Yes ( ) , and Fyn ( ) ) compared with Src ( ++ ) cells ( c Src ( ++ ) , Yes ( ) , and Fyn ( ) ) . ^^^ |
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| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Fyn / Yes and non canonical Wnt signalling converge on RhoA in vertebrate gastrulation cell movements . ^^^ Here , we show that morpholino ( MO ) mediated double knock down of the Fyn and Yes tyrosine kinases in zebrafish embryos impaired normal CE cell movements , resembling the silberblick and pipetail mutants , caused by mutations in wnt 11 and wnt 5 , respectively . ^^^ Co injection of Fyn / Yes and Wnt 11 or Wnt 5 MO was synergistic , but wnt 11 or wnt 5 RNA did not rescue the Fyn / Yes knockdown or vice versa . ^^^ Remarkably , active RhoA rescued the Fyn / Yes knockdown as well as the Wnt 11 knockdown , indicating that Fyn / Yes and Wnt 11 signalling converged on RhoA . ^^^ Our results show that Fyn and Yes act together with non canonical Wnt signalling via RhoA in CE cell movements during gastrulation . . ^^^ |
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| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| FYN and YES proteins were found in mouse eggs , but other SFKs were not detected ; based on this , we injected mouse eggs with a mixture of FYN and YES Src homology 2 ( SH 2 ) domains . ^^^ |
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| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| CSK overexpression caused a profound inhibition of NGF induced activation of FYN , YES , RAS , and ERK and inhibited neurite outgrowth , NGF stimulated integrin directed migration and blocked the NGF induced conversion of GDP RAC to its GTP bound active state . ^^^ |
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| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| CD95L also induced rapid activation of the Src family kinase Yes , being followed by activation of c Src , Fyn , and c Jun N terminal kinases ( JNK ) . ^^^ Only Yes and JNK activation were sensitive to N acetylcysteine , inhibitors of NADPH oxidase , PKCzeta , or sphingomyelinase , indicating that the CD95L induced ROS response is upstream of Yes and JNK but not of Fyn and c Src activation . ^^^ |
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| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| RT PCR revealed mRNA transcripts for FYN , c SRC , YES , FRK , and LYN . ^^^ Fyn , c Src , Yes , and Lyn were identified in cultured airway smooth muscle cells by immunoblot analysis . ^^^ |
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| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Src is not detectable in MA 10 cells , but two other prominent members of this family ( Fyn and Yes ) are present . ^^^ Moreover , activation of the LHR in MA 10 cells results in the stimulation of the activity of Fyn and Yes , and overexpression of either of these two tyrosine kinases enhances the LHR mediated phosphorylation of FAK Tyr 576 . ^^^ |
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| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| Using dominant negative mutants of c Src and Fyn and Src deficient SYF cells as well as by co immunoprecipitation studies , we can demonstrate that the M2R mediated transactivation of EGFR specifically involves Fyn but not c Src or Yes . ^^^ |
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| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| This requirement is unique for TRPC 3 because ( 1 ) mutation of the cognate tyrosines of the closely related TRPC 6 and TRPC 7 had no effect ; ( 2 ) TRPC 6 and TRPC 7 were activated in src , yes , and fyn deficient cells ; and ( 3 ) src , but not yes or fyn , rescued TRPC 3 activation in src , yes , and fyn deficient cells . ^^^ |
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| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| We further show that Src , Fyn and Yes have unique specificities towards these tyrosine residues . ^^^ Using cell lines deficient in Src , Fyn and Yes , we demonstrate that multiple Src family members can stimulate vaccinia induced actin polymerization and also uncover a role for Abl family kinases . ^^^ |
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| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| We expressed Fgr , Fyn , Hck , Lck , Lyn , and Yes as well as c Src in yeast and found that each kinase was active and induced growth suppression . ^^^ Nef strongly activated Hck , Lyn , and c Src but did not detectably affect Fgr , Fyn , Lck , or Yes . ^^^ |
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| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| The NMDAR is a substrate of src family tyrosine kinases , and reduced activity of src , fyn , yes and lck , but not lyn , was apparent in the absence of PTPalpha . ^^^ Together , these findings provide evidence that aberrant NMDAR associated functions in PTPalpha null mice are due to impaired NMDAR tyrosine phosphorylation resulting from the reduced activity of probably more than one of the src family kinases src , fyn , yes and lck . ^^^ |
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| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| We report here the map location of a Xiphophorus yes gene , YES 1 , in LG 6 , closest to the EGFR gene and the assignment of a fyn gene homologue to newly designated LG 15 , linked to the gene for cytosolic alpha galactosidase . ^^^ |
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| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07947 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
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