| Interacting proteins: P06729 and P06241 |
Pubmed |
SVM Score :0.56438123 |
| We now report that stimulation of T cells with mitogenic pairs of anti CD 2 mAbs enhanced the association of the Fyn polypeptide with the CD 2 complex , whereas stimulation with single anti CD 2 mAb had minimal effect . 0.56438123^^^ CD 2 has been shown previously to associate physically with the Src family protein tyrosine kinases p 56 ( lck ) and p 59 ( fyn ) . 0.53539067^^^ However , the inhibitory effect of the Fyn SH 2 domain on binding of the Fyn SH 3 domain to CD 2 was relieved by peptides containing a phosphorylated YEEI sequence that bound directly to the Fyn SH 2 domain . 0.52861183^^^ |
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| Interacting proteins: P06729 and P06241 |
Pubmed |
SVM Score :0.0 |
| Ligation of the CD 2 cell surface glycoprotein expressed on T lymphocytes and NK cells induces protein tyrosine phosphorylation and activation of the Src kinases , LCK and FYN . ^^^ |
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| Interacting proteins: P06729 and P06241 |
Pubmed |
SVM Score :0.0 |
| CD 2 has a relatively long cytoplasmic tail which associates with the src family tyrosine kinases , p 56 ( lck ) and p 59 ( fyn ) , and could potentially signal directly . ^^^ |
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| Interacting proteins: P06729 and P06241 |
Pubmed |
SVM Score :0.0 |
| Here we demonstrate that Lck and Fyn can be activated by proline motifs in the CD 28 and CD 2 proteins , respectively . ^^^ |
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| Interacting proteins: P06729 and P06241 |
Pubmed |
SVM Score :0.0 |
| A critical role for p 59 ( fyn ) in CD 2 based signal transduction . ^^^ To test the hypothesis that p 59 ( fyn ) , a second src family protein tyrosine kinase ( PTK ) expressed in T lymphocytes , might be an essential upstream component of the CD 2 signaling pathway , we generated human ( h ) CD 2 transgenic ( tg ) fyn ( + / + ) and fyn ( / ) mice . ^^^ In contrast , in the absence of p 59 ( fyn ) , these CD 2 initiated activities are markedly reduced , while TCR triggered proliferation is unaffected . ^^^ Several CD 2 pathway components regulated by p 59 ( fyn ) have been identified including phospholipase C gamma 1 ( PLC gamma 1 ) , Vav , protein kinase C theta isoform ( PKC theta ) , docking protein ( Dok ) , focal adhesion kinase ( FAK ) and Pyk 2 . ^^^ Of note , CD 2 dependent Dok but not linker from activated T cells ( LAT ) tyrosine phosphorylation requires p 59 ( fyn ) . ^^^ |
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| Interacting proteins: P06729 and P06241 |
Pubmed |
SVM Score :0.0 |
| While CD 2 induced p 62 ( dok ) tyrosine phosphorylation was independent of both the p36 / 38 membrane adapter protein linker of activated T cells ( LAT ) and the ZAP70 / Syk family of kinases , it was dependent upon the Src family of kinases including Lck and Fyn . ^^^ |
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| Interacting proteins: P06729 and P06241 |
Pubmed |
SVM Score :0.0 |
| NMR analysis shows that the Fyn but not the Lck tyrosine kinase SH 3 domain competes with CD2BP2 GYF domain binding to the same CD 2 proline rich sequence in vitro . ^^^ To test the in vivo significance of this competition , we used co immunoprecipitation experiments and found that CD2BP2 is the ligand of the membrane proximal proline rich tandem repeat of CD 2 in detergent soluble membrane compartments , but is replaced by Fyn SH 3 after CD 2 is translocated into lipid rafts upon CD 2 ectodomain clustering . ^^^ |
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| Interacting proteins: P06729 and P06241 |
Pubmed |
SVM Score :0.0 |
| Examination of protein kinases associating with these antigens showed that paradoxically , CD 2 coprecipitated the highest amount of Lck and Fyn . ^^^ CD 6 also associated with Lck , Fyn , and ZAP 70 , although at lower levels but additionally coprecipitated the Tec family kinase Itk , which is absent from CD 2 , CD 5 , and TCR complexes . ^^^ |
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| Interacting proteins: P06729 and P06241 |
Pubmed |
SVM Score :0.0 |
| They hypothesize that the key event in T cell activation is the formation of multimolecular complexes consisting of the TCR and several other polypeptides , including CD 4 or CD 8 , CD 2 , CD 5 and the associated tyrosine kinases p 59 ( fyn ) and p 56 ( lck ) . . ^^^ |
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| Interacting proteins: P06729 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|