| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| A 17 kb genomic clone encoding human p 56 Lck , a lymphocyte specific PTK of the Src related subfamily , has been isolated . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| Of the different PTK detected in NK cells , only p56lck has previously been shown to participate in NK cell activation . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| Vav also was a substrate for the p56lck PTK . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| CaM 1 cells that lack a functional form of the PTK Lck are unable to induce Band A through the T cell antigen receptor . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| We have previously shown that Lck plays a major role in the tyrosine phosphorylation of the TCR zeta chain and the ZAP 70 PTK . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| Unlike Syk , the PTK Lck activates JNK but does not cooperate with Rac , resulting in weak AP 1 and NF AT activation . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| Consistent with signal transduction through p56lck or p59fyn , inhibition of PTK signaling reduced phosphorylation 50 % . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| This review summarizes our current knowledge on Lck , a member of the Src family of PTK , and its viral interaction partners . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| Paxillin phosphorylation and association with Lck and Pyk 2 in anti CD 3 or anti CD 45 stimulated T cells . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| RAFTK also co immunoprecipitates with the SH 2 domain of Lck and with the cytoskeletal protein paxillin through its COOH terminal proline rich domain . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| We report that myr PKB renders proliferative responses of thymocytes more sensitive to TCR signals by increased and sustained activation of Src kinase Lck and the extracellular signal regulated kinase / mitogen activated protein kinase pathway . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| Treatment with genistein , a PTK inhibitor , revealed that the PTK activity of p56lck might not be involved in this regulatory effect of p56lck on CD 4 internalization . . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| Tyrosine phosphorylation of several intracellular proteins is observed very early during T cell activation . p56lck , a non receptor src like protein tyrosine kinase ( PTK ) which is associated with the intracellular domains of CD 4 and CD 8 co receptors , has been implicated in these early signal transduction events . ^^^ Furthermore , recent experiments indicate that the receptor phosphotyrosine phosphatase , CD 45 , might be important in the regulation of p56lck PTK activity and that its expression is required for the generation of second messenger molecules following TCR triggering . ^^^ Further , this provides evidence that p56lck PTK is a substrate of CD 45 phosphotyrosine phosphatase in vivo and thereby supports the idea that CD 45 is an early regulator of T cell activation involved in the modulation of the coupling of receptor triggered events to intracellular signalling pathways . . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| In parallel with the changes in IL 2R beta expression , the SRC like protein tyrosine kinase ( PTK ) p 56 LCK could not be detected in IL 3 dependent cells , but was abundant in the IL 2 dependent cells and underwent markedly increased autophosphorylation in response to IL 2 . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| In this study , we have characterized a spontaneous variant of the IL 2 dependent cytotoxic T cell line , CTLL 2 , which contains no detectable lck derived mRNA transcripts , protein , or PTK activity . ^^^ These results indicate that p56lck expression is not obligatory for IL 2 mediated T cell growth stimulation ; however , this PTK plays a central role in the generation T cell mediated cytotoxic responses . . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| Furthermore , immunoblot assays determined that the levels of p 56 LCK protein were unaltered by IL 2 treatment , indicating that the measured elevations in LCK kinase activity reflected an increase in the specific activity of this PTK . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| In this study , we have demonstrated that stress stimuli enhance protein tyrosine phosphorylation ( PTyr P ) , modulate protein tyrosine phosphatase ( PTPase ) activity , activate the src family protein tyrosine kinase ( PTK ) , p56lck , and enhance glucose uptake in human peripheral blood mononuclear cells . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| Since none of the seven TcR CD 3 subunits exhibits a recognizable kinase domain , it seems likely that the receptor complex is associated with an intracellular PTK . p59fyn and the T lymphocyte specific p56lck are two intracellular , non receptor , cell membrane associated PTK of the src family expressed in T lymphocytes . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| Recently , interleukin 2 ( IL 2 ) has been shown to induce increased activity of the p56lck protein tyrosine kinase ( PTK ) in T cell and natural killer cell lines , and evidence for a direct interaction between the p 75 subunit of the IL 2 receptor ( IL 2R ) and this src family kinase has been reported . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| Human HCK , C SRC ( encoding p 60 Src PTK ) , C FGR and LCK ( encoding p 56 Lck , PTK ) genes thus share very similar exon / intron structures for the conserved exons . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| Recently , it was reported that the IL 2R ( whose p 75 beta subunit shares sequence homology with a known murine IL 3R subunit and a common beta subunit of the human IL 3R and granulocyte macrophage colony stimulating factor [ GM CSF ] receptors ) can physically associate with and regulate the activity of the SRC family PTK , p 56 LCK . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| The T cell specific protein tyrosine kinase ( PTK ) , p56lck , has been implicated in the tyrosine phosphorylation of TCR zeta . ^^^ Genistein inhibited the T cell PTK , p56lck , in a dose dependent fashion with an ID 50 = 40 microM . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| Two other T cell integral membrane proteins , CD 4 and CD 8 , are associated with the protein tyrosine kinase ( PTK ) , lck . ^^^ In this study we use digitonin to solubilize a murine T cell hybridoma and demonstrate that antibodies binding extracellular but not intracellular domains of the TCR specifically coprecipitate only the fyn PTK and not lck or yes , two other kinases found in these cells . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| Recent findings by several groups strongly suggest a functional role for the gene product p56lck protein tyrosine kinase ( PTK ) in the activation of normal T cells . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| We report here the isolation of a human T cell specific cDNA clone ( YT 16 ) that encodes a protein with extensive homology to a family of transformation and proliferation related protein tyrosine kinases ( PTK ) . ^^^ Around the PTK catalytic domain , the homologies increase to 70 80 % between the deduced protein sequence of YT 16 and those of the PTK viral oncogenes . ^^^ Of special interest is the extensive homology between the deduced protein sequence of YT 16 and that of a recently reported murine B and T lymphocyte specific PTK , lskT . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| In fact , at least four protein tyrosine kinases ( PTKs ) are physically associated with IL 2R : p56lck ( and its members ) , Syk PTK , and the Janus kinases , Jak 1 and Jak 3 . cDNA expression studies revealed that the activation of these PTKs is critical for IL 2 induced proliferative signal transmission . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| The BCR associates with two classes of tyrosine kinase : Src family kinase ( Src protein tyrosine kinase [ PTK ] ; Lyn , Fyn , Blk , or Lck ) and Syk kinase . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| By using specific antisera , the presence of p56lck and p59fyn protein tyrosine kinases ( PTK ) was clearly demonstrated in the CD 50 immunoprecipitates . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| We propose that p72syk may act as an immediate receptor activated kinase upstream of the related p70zap PTK and the src family PTKs p56lck and p59fyn in T cells and that these src family PTKs act as signal amplifiers . . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| Individual replacement of the two cysteine residues within the first 10 amino acids of p59fyn and p56lck with serine indicated that Cys 3 was the major determinant of palmitoylation , as well as association of the PTK with glycosyl phosphatidylinositol anchored proteins . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| Activation of lymphocytes through the T cell receptor ( TCR ) in collaboration with cell surface accessory molecules results in rapid increases in tyrosine phosphorylation , probably through the recruitment and activation of src family protein tyrosine kinases ( PTK ) such as lck and fyn which have been implicated in mediating the proximal signalling events mediated through the TCR . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| Although several lines of evidence suggest involvement of protein tyrosine kinase ( PTK ) activity in this process , the role of src family PTK p561ck ( lck ) is not known . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| The c src gene family has nine known members ( blk , c fgr , fyn , hck , lck , lyn , c src , c yes and yrk ) , each encoding a cytoplasmic protein tyrosine kinase ( PTK ) believed to be involved in signal transduction . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| Two classes of protein tyrosine kinases ( PTK ) are utilized by the T cell antigen receptor ( TcR ) / CD3 complex for initiation of the signaling cascade , the Src family PTK p56lck and p59fyn , and the Syk family PTK p70zap and p72syk . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| The activity of baculoviral expressed ZAP 70 was up regulated 10 fold when ZAP 70 was co infected and phosphorylated by the src family PTK , lck . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| Due to its association with the protein tyrosine kinase ( PTK ) p56lck , CD 4 is believed to transduce a signal and support CD 3 activation of T cells . ^^^ Furthermore , using HPB clones deficient in CD3 / PTK association , it has been found that the presence of CD4 / p56lck does not overcome the defect in signalling . ^^^ Together , the present results indicate that CD 3 linked PTK ( s ) plays a primary role in the induction of signalling through TCR / CD3 , and the presence of CD4 / p56lck is neither necessary nor sufficient to elicit these events . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| We demonstrate here that LFA 1 is physically associated in the YT human NK like cell line cells with a PTK ( s ) that is distinct from Src family PTKs such as Lck , Fyn , or Lyn . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| Since this src related PTK has been shown to enhance TCR / CD3 mediated T cell responsiveness , here we investigated whether activation of Lck by IL 2 could contribute to enhance TCR / CD3 mediated T cell functions . ^^^ Interaction of the interleukin 2 receptor ( IL 2R ) beta chain with the lymphocyte specific protein tyrosine kinase ( PTK ) , p56lck , has led to the speculation that p56lck participates in growth signal transduction . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| The ectodomains of the T cell surface glycoproteins CD 4 and CD 8 bind to membrane proximal domains of MHC class 2 and class 1 molecules , respectively , while both cytoplasmic domains interact with the protein tyrosine kinase ( PTK ) p56lck ( lck ) through a shared cysteine containing motif . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| We have studied the effects of the CD 4 associated Lck molecule on TCR mediated activation of the protein tyrosine kinase ( PTK ) pathway in a murine T cell hybridoma . ^^^ Cross linking of the CD4 / Lck chimera to a CD8 / zeta chimeric molecule also facilitated induction of the PTK pathway with anti CD 8 antibodies . ^^^ The effects of CD4 / Lck on tyrosine phosphorylation and activation of PLC correlated well with the effects on PTK activation . ^^^ Our results suggest that the Lck molecule positively regulates the TCR coupled PTK pathway by phosphorylating tyrosines on the TCR associated CD 3 zeta polypeptides . . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| Second , a differential activation of p56lck , a src related PTK , was observed after stimulation with D66 / T11 , and GT2 / T11 ( 1 ) . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| Examination of these inhibitors in immunoprecipitated p56lck , epidermal growth factor receptor ( EGFR ) , and c erb B 2 / HER 2 / neu PTK preparations showed that compound 12 ( IC 50 = 60 nM ) was one of the most potent p56lck inhibitors reported to date . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| Moreover , one inhibitor ( genistein ) revealed the existence of a PTK which down regulates specifically calcium mediated signaling at a point downstream of the PTK p56lck but upstream of calcium mobilization . . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| Genistein , an isoflavanoid compound , a selective inhibitor of protein tyrosine kinase ( PTK ) , inhibited PHA stimulated bovine peripheral blood mononuclear cell proliferation ( PBMC ) , interleukin 2 ( IL 2 ) production , phosphorylation of PTK p56lck . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| With respect to the involvement of PTK ( s ) in IL 2R mediated signal transduction , it has been demonstrated that p56lck , a member of the src family PTKs interacts with the IL 2R beta . ^^^ In fact , IL 2 stimulation increases the PTK activity of p56lck . ^^^ Interestingly the ' serine rich ' and ' acidic ' regions of IL 2R beta are both required for the PTK activation of p56lck . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| Coexpression of the PTK p59fynT , but not p56lck , with zeta or with a homodimeric TCR beta zeta fusion protein produced tyrosine phosphorylation of both zeta and phospholipase C ( PLC ) gamma 1 , as well as calcium ion mobilization in response to receptor cross linking . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| The PTK inhibitory activity of the crude fractions was determined by measuring their inhibition of p56lck partially purified from bovine thymus using angiotensin 1 as a substrate . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| We report here that the human Fc gamma RIIIA is complexed with p56lck , a src family PTK previously found associated with the CD 4 and CD 8 receptors on T cells . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| In the present case , however , the resulting salicyl containing bicyclics exhibited extremely poor inhibitory potency when examined against autophosphorylation of immunoprecipitated p56lck PTK preparations . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| Studies of decay accelerating factor ( DAF ) or CD 59 in either the C 32 cell line or the HeLa cell line transfected with PTK cDNA demonstrated that the GPI anchored proteins associated noncovalently with p56lck and p59fyn but not with p60src . ^^^ A chimeric PTK consisting of the 10 amino terminal residues of p56lck or p59fyn replacing the corresponding amino acids in p60src was sufficient for association with DAF , but the converse constructs containing the first 10 amino acids of p60src plus the remainder of p56lck or p59fyn did not associate with DAF . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| To examine the mechanisms underlying p56lck PTK activation by IL 2 , we established a mouse pro B cell line , BAF B 03 , expressing both IL 2R beta ( either the wild type or mutant forms ) and mouse p56lck at high levels . ^^^ BAF B 03 cells expressing a mutant IL 2R beta chain lacking an ' acidic ' region of the cytoplasmic domain , previously shown to be essential for association with p56lck , fail to induce p56lck PTK activation upon IL 2 stimulation . ^^^ This suggests that the association of p56lck with the IL 2R beta chain , despite its low stoichiometry , is required for the activation of cellular p56lck PTK upon IL 2 stimulation . ^^^ Hence , physical association of p56lck with the IL 2R beta chain is not by itself sufficient to permit IL 2 mediated regulation of this PTK . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| Meanwhile , another cytoplasmic region of IL 2R beta , the `` acidic ' ' region , is responsible for its physical association with an src family protein tyrosine kinase ( PTK ) , p56lck and is critical for activating the p56lck PTK following IL 2 stimulation . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| However , P 120 was only minor phosphorylated on tyrosine upon receptor crosslinking in A2 . 01 / CD4 ( cyt 401 ) , a derivative line expressing a truncated CD 4 coreceptor lacking its cytoplasmic domain which binds the p56lck protein tyrosine kinase ( PTK ) . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| We provide evidence that the Src family PTK Lck is responsible for regulating the constitutive tyrosine phosphorylation of the TCR zeta subunit in murine thymocytes . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| CD 28 induced tyrosine phosphorylation and PI 3 K activation was independent of p56lck protein tyrosine kinase ( PTK ) activity ( previously reported to be associated with CD 28 ) and was insensitive to inhibition by the PTK inhibitor herbimycin A . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| These results are the first report where dual activation of the Lck and Fyn PTK does not result in normal downstream signaling through the ZAP PTK , We suggest by analogy to SCID resulting from ZAP 70 mutations , that the dissociation of upstream PTK activation from ZAP 70 signaling contributes to T cell depletion by HIV and to the development of AIDS . . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| The protein tyrosine kinase ( PTK ) Lck phosphorylates the zeta chain , which in turn associates with another PTK , ZAP 70 , and stimulates its phosphorylation activity . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| The role of the gp 120 CD4 Lck interaction in HIV related PTK activation was determined using gp 120 treated Jurkat cells and HIV infection of JCaM 1 . 6 cells , a Jurkat derived cell line that lacks p56lck . ^^^ HIV related src family PTK activation was not a function of the gp 120 CD4 Lck interaction and occurred in the presence of 10 mmol / l zidovudine indicating that reverse transcriptase and activation of the HIV genome is not required . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| Altogether , these findings suggest that CD 2 could bypass the PTK ZAP 70 for PLCgamma 1 activation and involves a preferential cascade comprising p56lck and a p 62 protein , possibly acting as an anchor molecule . . ^^^ The T cell specific protein tyrosine kinase ( PTK ) ZAP 70 is believed to play a key role in early tyrosine phosphorylations of cellular proteins induced by CD 3 stimulation . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| Although IL2R beta and gamma chains lack an intrinsic protein tyrosine kinase ( PTK ) domain , these chains recruit various non receptor type PTKs , such as p56lck ( and other Src family PTKs ) , Jak PTKs and Syk PTKs . ^^^ These signalling pathways , except that for bcl 2 pathway , appear to be mediated by multiple PTKs : p56lck is critical for the induction of the c fos / c jun genes , the activation of Syk PTKs results in the induction of the c myc gene and Jak 3 PTK is required for the induction of both c fos and c myc genes . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| One of the earliest steps in the activation pathway is thought to involve the Src family PTKs , p 56 ( lck ) ( Lck ) and p 59 ( fyn ) ( Fyn ) ; however , the precise contribution of each PTK in TCR mediated signaling remains incompletely understood . ^^^ In this report , we demonstrate that specific inhibition of Lck expression in Th 2 cells , in the presence of normal levels of functional Fyn PTK , has profound consequences on multiple events following TCR stimulation , including an altered pattern of tyrosine phosphorylated substrates , defective phosphorylation of TCR zeta and ZAP 70 , defective Ca2+ mobilization , and a approximately 90 % reduction in proliferative responses to antigenic and mitogenic stimuli . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| In the course of these experiments , we found that the Syk PTK , but not the ZAP 70 PTK , is able to mediate TCR signaling independently of CD 45 and of Lck . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| To study the role of ZAP 70 catalytic activity in this process , a substrate capable of distinguishing between the activities of ZAP 70 and other PTKs would be useful , especially since it has recently been shown that ZAP 70 interacts with another T cell PTK , Lck . ^^^ |
|
| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| Using a somatic genetic model , we first define a requirement for the Src family protein tyrosine kinase ( PTK ) Lck in mediating KIR tyrosine phosphorylation . ^^^ These results suggest sequential roles for Lck and SHP 1 in the inhibition of PTK following MHC recognition by NK cells . . ^^^ |
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| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| This finding and other observations led us to hypothesize that the effects of gp 120 are mediated through p 56 ( lck ) , a PTK noncovalently associated with CD 4 . ^^^ |
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| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| We examined the effects of gp 120 on TCR CD 3 induced phosphorylation and activation of the src type protein tyrosine kinases ( PTK ) , fyn and lck . gp 120 caused minimal changes in lck phosphorylation or lck enzymatic activity , but preincubation of Jurkat cells with gp 120 for 20 min strongly inhibited TCR CD 3 mediated phosphorylation and activation of lck and fyn , as well as phosphorylation of CD 3 zeta . ^^^ |
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| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| We used mice deficient in Lck , Fyn , or both Lck and Fyn to analyze the role that these src family PTK play in IEL development . ^^^ Interestingly , a small population of TcR gammadelta+ CD 8 alpha alpha+ cells are apparent in lck / fyn / animals , demonstrating that TcR alphabeta+ CD 8 alpha alpha+ and TcR gammadelta+ CD8alpha alpha+ IEL have distinct PTK requirements for their development or expansion . ^^^ |
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| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| To determine whether CD 3 associated p59fyn , or CD 4 and CD 8 associated p56lck phosphotyrosine kinases ( PTK ) were involved , we assayed kinase activity in lymphocyte lysates immunoprecipitated with anti p56lck and p59fyn mAbs . ^^^ |
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| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| T cell antigen receptor ( TCR ) engagement activates multiple protein tyrosine kinases ( PTKs ) , including the Src family member , Lck , and the Syk related PTK , ZAP 70 . ^^^ |
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| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| All glycosylphospha tidylinositol ( GPI ) anchored proteins ( CD 14 , CD16B , CD 48 , CD 59 , CD 87 and GPI anchored versions of CD 2 and CD 25 ) and protein tyrosine kinase ( PTK ) Lck but not transmembrane proteins ( CD 2 , CD 4 , CD 5 , CD 6 , CD 8 ) and PTK ZAP 70 were in part localized in buoyant , lipid rich , detergent resistant membrane GPI microdomains of the COS cells . ^^^ Moreover , the N terminal double acylation of the PTK Lck appears to be functional also in COS cells and targets the enzyme to the membrane GPI microdomains implicated in receptor signalling . . ^^^ |
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| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| While the Src PTKs mediate the phosphorylation of TCR associated signaling subunits and the phosphorylation and activation of the Syk PTKs , the lack of a constitutively active Syk PTK has prohibited the analysis of Lck function downstream of these initiating signaling events . ^^^ |
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| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| This antiviral effect requires the integrity of the cytoplasmic tail of CD 4 which is known to act as a signal transduction region through its association with the protein tyrosine kinase ( PTK ) p56lck . ^^^ |
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| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| Selectivity studies of peptides 23 30 toward p60c src , Lyn , and Lck PTK showed in general high Lyn / Src and moderate Lck / Src selectivity ratios . ^^^ We found that the chi 1 space constraints of the specialized amino acids , introduced at position 3 of the peptide lead 23 , were not as important as the configuration of the Calpha of that residue to recognize the subtle chemical environment surrounding the active site of Src and Lck PTK , as reflected on the obtained Lck / Src selectivity ratios . . ^^^ |
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| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| Two broad specificity biotinylated PTK peptide substrates were identified with optimum kinetic properties , allowing members from eight distinct classes of enzymes , including transmembrane ( epidermal growth factor receptor ( EGFR ) , fibroblast growth factor receptor , insulin receptor , and platelet derived growth factor receptor ) and cytoplasmic ( p 43 ( abl ) , p 56 ( lck ) , p 60 ( src ) , and p 93 ( fes ) ) PTKs , to be analyzed . ^^^ |
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| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| However , p 59 ( fyn ) immuno precipitates obtained from unstimulated peripheral blood SLE T cells showed enhanced PTK activity as compared to controls , whereas the PTK activity of p 56 ( lck ) and ZAP 70 molecules was comparable in both groups . ^^^ |
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| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| Following T cell antigen receptor ( TCR ) engagement , the protein tyrosine kinase ( PTK ) ZAP 70 is rapidly phosphorylated on several tyrosine residues , presumably by two mechanisms : an autophosphorylation and a trans phosphorylation by the Src family PTK Lck . ^^^ |
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| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| As a result of interaction with epithelial cells in the thymic cortex , immature CD 4 ( + ) 8 ( + ) ( double positive , DP ) thymocytes express relatively few T cell receptors ( TCRs ) and contain diminished numbers of coreceptor associated p 56 ( lck ) ( lck ) PTK molecules . ^^^ |
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| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| Here we show that ligation of the A6H antigen with mAb induces tyrosine phosphorylation of the Lck protein tyrosine kinase ( PTK ) . ^^^ |
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| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| By overexpressing the two proteins , we show that the constitutive phosphorylation of Sam 68 in vivo directly correlates with cellular Fyn levels , but not with Lck expression , despite the capacity of the PTK to strongly phosphorylate the molecule in vitro . ^^^ |
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| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| In the present study , we investigated the expression of the zeta chain molecule and the p 56 ( lck ) and p 59 ( fyn ) protein tyrosine kinase ( PTK ) levels in peripheral blood T lymphocytes ( T PBL ) from patients with advanced gastric carcinomas ; for this , flow cytometric analysis and immunoblotting , respectively , were used . ^^^ |
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| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| The proline , glutamic acid , serine and threonine enriched protein tyrosine phosphatase PEP , which is expressed primarily in hematopoietic cells , was recently discovered to be physically associated with the 50 kDa cytosolic protein tyrosine kinase ( PTK ) Csk , an important suppressor of Src family PTK , including Lck and Fyn in T cells . ^^^ |
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| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| With this assay we demonstrate that the distal promoter element regulating expression of lck , a src PTK essential for T cell development and activation , is similarly regulated during ontogeny of T and B cells . ^^^ Moreover , although transcripts encoding the src PTK fyn accumulate at 0 . 1 % of lck mRNA levels in thymocytes , diminished activity of the lck distal promoter in the B cell background brings lck and fyn transcript levels to near equivalence in this population . ^^^ |
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| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| Quantitative structure activity relationship ( QSAR ) studies on 104 flavonoid derivatives as p56lck protein tyrosine kinase ( PTK ) inhibitors were performed , using a large number of molecular descriptors calculated by CODESSA software . ^^^ |
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| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| Both the direct and indirect FP PTK immunoassays have been compared with a more commonly used ( 32 ) PO ( 4 ) transfer assay and validated using lymphoid T cell protein tyrosine kinase ( Lck ) . ^^^ |
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| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| Preincubation of HOBIT cells with damnacanthal ( DAM ) , which inhibits p56lck , blocked ETOH mediated PTK activity ; whereas , preincubation with herbimycin A , which inhibits pp60src , did not . ^^^ |
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| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| The present study examined the effects of TGF beta on the activation of protein tyrosine kinases ( PTK ) P56lck , P59fyn , and Zap 70 , and protein tyrosine phosphatases ( PTP ) SHP 1 and SHP 2 . ^^^ |
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| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| Thrombin induced ERK and p 38 MAPK activation was more pronounced in p56Lck deficient cells indicating that this PTK exerts a negative control on MAPK activity . ^^^ |
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| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| Engagement of the T cell antigen receptor ( TCR ) induces the transphosphorylation of the zeta chain associated protein of 70 , 000 Mr ( ZAP 70 ) protein tyrosine kinase ( PTK ) by the CD4 / 8 coreceptor associated Lck PTK . ^^^ |
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| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| HIV SUgp 120 ( HIV surface glycoprotein ) , T cell receptor ( TCR ) CD4+ and co receptors promote aggregation of these lipid `` rafts ' ' which concentrate the Src family tyrosine kinases SFKs ( PTK , Lyn , Fyn , Lck ) , GPI ( glycosyl phosphatidylinositol ) anchored proteins , and phosphatidylinositol kinases PI ( 3 ) K and PI ( 4 ) K , inducing cell signalling . ^^^ |
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| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| These unpublished data indicate that sHLA I / CD8 ligation elicits the phosphorylation of p56lck protein thyrosin kinase ( PTK ) associated with CD 8 cytoplasmic domain in the absence of any other TCR derived signal , the activation of syk like ZAP 70 PTK and protein kinase C , and extracellular calcium influx . ^^^ |
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| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| Molecular analysis of the biochemical pathways responsible for FasL up regulation showed that sHLA I / CD8 interaction firstly induced the recruitment of src like p 56 ( lck ) and syk like Zap 70 protein tyrosine kinases ( PTK ) . ^^^ |
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| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| T cell receptor engagement induces tyrosine phosphorylation of FAK and Pyk 2 and their association with Lck . ^^^ The increase in tyrosine phosphorylation of both FAK and Pyk 2 , however , occurs in Lck deficient cells suggesting that phosphorylation of both of these kinases does not require Lck . ^^^ Taken together , these results suggest that FAK and Pyk 2 , perhaps in coordination with Lck , play a role in T cell activation . . ^^^ |
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| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| Regulation of Pyk 2 expression by p 56 ( Lck ) in Jurkat T lymphocytes . ^^^ CaM1 cells with p 56 ( Lck ) restored expression of the multiple forms of Pyk 2 . ^^^ Using RT PCR , we found that both species of the alternatively spliced mRNA for Pyk 2 were present in all of the lines regardless of their ability to express CD 45 or p 56 ( Lck ) protein . ^^^ When p 56 ( Lck ) immunoprecipitates were immunoblotted with anti Pyk 2 , only the higher mobility form of Pyk 2 immunoprecipitated with p 56 ( Lck ) . ^^^ |
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| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| First , Lck activity was required for TCR induced Pyk 2 translocation , but not for TCR induced Pyk 2 activation . ^^^ In addition , Lck activity alone was insufficient to induce Pyk 2 reorientation and activation , requiring the presence of at least one intact immunoreceptor tyrosine based activation motif ( ITAM ) . ^^^ Despite the dependence on functional Lck and on phosphorylated ITAM for Pyk 2 translocation , the ITAM binding tyrosine kinase zeta associated protein 70 ( ZAP 70 ) was not essential . ^^^ |
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| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| This adhesion induces recruitment and activation of the focal adhesion kinase Pyk 2 , the cytoskeleton linker paxillin , and the Src kinases Lck and Fyn in the contact site . ^^^ |
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| Interacting proteins: Q14289 and P06239 |
Pubmed |
SVM Score :0.0 |
| These results implicate Dok 1 , Nck , Crk L , and Src kinases especially Lck , Pyk 2 , Zap 70 , Vav , and Ras GAP in intracellular signaling by SDF 1alpha / CXCL12 , and they suggest that Dok 1 plays an important role in SDF 1alpha / CXCL12 induced chemotaxis in T cells . . ^^^ |
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