| Interacting proteins: P42336 and P06239 |
Pubmed |
SVM Score :0.81453782 |
| We also show that p56lck directly activates PI3K purified from transfected COS 1 cells , indicating that other T cell specific proteins are not required for the process . 0.81453782^^^ |
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| Interacting proteins: P42336 and P06239 |
Pubmed |
SVM Score :0.0 |
| We have used in vitro binding studies employing bacterially expressed glutathione S transferase p56lck fusion proteins and cell extracts to map regions on p56lck that are involved in binding to phosphatidylinositol 3 ' kinase ( PI3K ) . ^^^ Deletions within the SH 3 domain of p56lck abolished binding of PI3K activity from T cell lysates , whereas deletion of the SH 2 domain caused only a slight reduction in the level of PI3K activity bound to p56lck sequences . ^^^ The binding of PI3K from T cell extracts to p56lck was not blocked by antiphosphotyrosine antibodies , but p56lck bound PI3K activity was sensitive to phosphatase treatment . ^^^ The SH 3 domain of p56lck also bound the majority of PI3K activity from uninfected chicken embryo fibroblasts . ^^^ However , a drastically different binding specificity was observed with use of extracts of Rous sarcoma virus 5 src transformed cells , in which the majority of PI3K activity bound to the SH 2 domain of p56lck in a phosphotyrosine dependent manner . ^^^ |
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| Interacting proteins: P42336 and P06239 |
Pubmed |
SVM Score :0.0 |
| On the other hand , full length Lck overexpressed in Sf 9 cells , which is tyrosine phosphorylated at least on two sites , can interact in vitro with the SH 2 from Lck , phospholipase C ( PLC ) gamma 1 , p 85 ( the regulatory subunit of phosphatidyl inositol 3 kinase ( PI3K ) ) and Nck and with the full length Grb 2 . ^^^ |
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| Interacting proteins: P42336 and P06239 |
Pubmed |
SVM Score :0.0 |
| The relative levels of phosphatidylinositol 3 ' kinase ( PI3K ) activity were measured in interleukin 2 ( IL 2 ) dependent helper ( HT 2 ) and cytolytic ( CTLL 2 ) T cell clones that had been stably transfected with expression plasmids encoding either the normal p 56 Lck kinase or a mutant version of this kinase , p 56 Lck ( Y505F ) , that has constitutively high levels of kinase activity . ^^^ Stimulation of untransfected T cells or of transfected T cells containing increased levels of normal p 56 Lck resulted in an approximate doubling of the relative amounts of total cellular PI3K activity . ^^^ In contrast , T cells producing the activated version of p 56 Lck contained levels of PI3K activity comparable to or slightly higher than those found IL 2 stimulated control cells , even in the absence of IL 2 . ^^^ Assessments of the relative levels of PI3K activity in immunoprecipitates prepared with the use of anti phosphotyrosine specific antibodies revealed constitutively high levels of anti phosphotyrosine immunoprecipitable PI3K activity in T cells containing p 56 Lck ( Y505F ) , as opposed to T cells containing normal p 56 Lck where increases in anti PY immunoprecipitable PI3K activity were IL 2 inducible . ^^^ IL 2 stimulation of T cells containing the normal p 56 Lck kinase resulted in marked increases in the relative amounts of PI3K activity and p 85 that were coimmunoprecipitated when using anti p 56 Lck antibodies . ^^^ |
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| Interacting proteins: P42336 and P06239 |
Pubmed |
SVM Score :0.0 |
| Furthermore , PI3K is recruited to CD 28 in JCaM 1 cells which lack LCK and in which EMT / ITK is not activated by ligation of CD 28 . ^^^ Thus optimal activation of LCK or EMT / ITK is not obligatory for recruitment of PI3K to CD 28 and thus is also not required for tyrosine phosphorylation of the YMNM motif in CD 28 . ^^^ |
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| Interacting proteins: P42336 and P06239 |
Pubmed |
SVM Score :0.0 |
| Ligation of the TCR or CD 28 induces activation of phosphatidylinositol 3 kinase ( PI3K ) , the TEC family protein tyrosine kinase , EMT / ITK / TSK ( EMT ) , and the SRC family tyrosine kinase , LCK . ^^^ Further , inhibition of PI3K markedly decreased EMT in vitro autokinase activity induced by activated LCK . ^^^ In contrast , PI3K inhibitors did not alter CD 28 or CD 3 cross linking or LCK induced EMT phosphorylation . ^^^ CD 28 induced association of EMT with PI3K also requires functional expression of LCK . ^^^ |
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| Interacting proteins: P42336 and P06239 |
Pubmed |
SVM Score :0.0 |
| We conclude that HIV 1 Nef plays a critical role in multiple processes in CD 4 down regulation : ( 1 ) disrupting the CD 4 p56 ( lck ) complex on the cell surface to allow CD 4 internalization and ( 2 ) diverting the internalized CD 4 to a lysosomal pathway for its degradation , likely through a PI3K activity . . ^^^ |
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| Interacting proteins: P42336 and P06239 |
Pubmed |
SVM Score :0.0 |
| The phosphatidylinositol 3 kinase ( PI3K ) and the tyrosine phosphatase SHP 1 are two Lck substrates that have been implicated in TCR signaling . ^^^ Co expression of SHP 1 and PI3K with a constitutively activated form of Lck in COS 7 cells demonstrated the carboxyl terminal SH 2 domain of PI3K to inducibly associate with the full length SHP 1 protein . ^^^ |
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| Interacting proteins: P42336 and P06239 |
Pubmed |
SVM Score :0.0 |
| We present evidence that endogenous Notch 1 associates with p 56 ( lck ) and PI3K but that Akt / PKB does not co immunoprecipitate with the Notch1 . p56 ( lck ) . ^^^ |
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| Interacting proteins: P42336 and P06239 |
Pubmed |
SVM Score :0.0 |
| Interestingly , we also found that both Smad 2 and Smad 3 interact with the Lck type 1 SH 2 domain , but not the PI3K type 3 SH 2 domain . ^^^ |
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| Interacting proteins: P42336 and P06239 |
Pubmed |
SVM Score :0.0 |
| CD 28 induced tyrosine phosphorylation and PI 3 K activation was independent of p56lck protein tyrosine kinase ( PTK ) activity ( previously reported to be associated with CD 28 ) and was insensitive to inhibition by the PTK inhibitor herbimycin A . ^^^ |
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| Interacting proteins: P42336 and P06239 |
Pubmed |
SVM Score :0.0 |
| Experiments using p56lck deficient or p56lck reconstituted Jurkat clones and the tyrosine kinase inhibitor herbimycin A revealed that tyrosine phosphorylation and activation of PI 3 K by CD 95 depends on expression of Src like tyrosine kinases , in particular p56lck . ^^^ |
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| Interacting proteins: P42336 and P06239 |
Pubmed |
SVM Score :0.0 |
| In contrast , mutation at the C terminal tyrosine of CD 3 epsilon ITAM did not prevent phosphorylation at the N terminal tyrosine , nor its association with Lck , or p 85 PI 3 K regulatory subunit . ^^^ |
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| Interacting proteins: P42336 and P06239 |
Pubmed |
SVM Score :0.0 |
| In this study , four colour flow cytometry was applied to study the surface bound molecules TCRalphabeta , CD 28 , CD 152 and CD 154 involved in T cell signalling and the signal transduction molecules CD3zeta , p56lck , p59fyn , ZAP 70 and phosphatidyl inositol 3 kinase ( PI 3 k ) as well as the intracellular cytokines interferon gamma ( IFN gamma ) , interleukin ( IL ) 4 and IL 2 as a functional read out of non stimulated and superantigen ( staphylococcus enterotoxin B ) stimulated blood T cells of multiple myeloma ( MM ) patients at different stages of the disease . ^^^ There was a marked reduction , particular in advanced stage MM , in the proportion of CD 4 and CD 8 cells expressing CD 28 , CD 152 , CD3zeta , p56lck , ZAP 70 and PI 3 k . ^^^ |
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| Interacting proteins: P42336 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42336 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42336 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42336 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42336 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42336 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42336 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42336 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
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