Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.71577945
Disruption of CD 8 dependent negative and positive selection of thymocytes is correlated with a decreased association between CD 8 and the protein tyrosine kinase , p56lck . 0.71577945^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :1.1412518
Avian CD 4 and CD 8 interact with a cellular tyrosine protein kinase homologous to mammalian p56lck . 1.1412518^^^ The results presented in this report demonstrate that the avian CD 4 and CD 8 homologs are physically associated with a cellular tyrosine protein kinase related to mammalian p56lck . 0.97621228^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.88724029
Association of CD 8 with p56lck is required for early T cell signalling events . 0.88724029^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.7547167
The functions of CD 8 in the TCR complex are thought to be signaling through the association of CD 8 with the protein tyrosine kinase p56lck and adhesion to MHC class 1 through the alpha 3 domain . 0.7547167^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.56650935
The common sequence motif shared by CD 4 and CD 8 alpha contains two cysteines , and mutation of either cysteine in the CD 4 sequence eliminated binding of p56lck . p56lck also contains two cysteine residues within its CD 4 CD8 alpha binding domain , and both are critical to the interaction with CD 4 or CD 8 alpha . 0.56650935^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.83913148
The cytoplasmic domain of the CD 8 alpha chain is required for its interaction with p56lck . 0.83913148^^^ The present report using cytoplasmic deletion mutants of the CD 8 alpha chain gene has extended our observations to demonstrate unequivocally that the cytoplasmic domain of the CD 8 alpha chain is responsible for interaction with p56lck . 0.7513451^^^ Recent studies have shown that CD 4 and CD 8 are physically associated with a T cell specific protein tyrosine kinase p56lck . 0.69902669^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.85828922
The T lymphocyte co receptors of MHC glycoproteins CD 4 and CD 8 are known to be associated with the protein tyrosine kinase Lck via cysteine containing sequences in the cytoplasmic domains of CD 4 and CD 8 and in the N terminal domain of Lck . 0.85828922^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :1.1430829
The cytoplasmic segment of the CD 8 alpha polypeptide includes both a cysteine containing motif that is required for its association with the tyrosine kinase p56lck , and two serine residues which are likely to be phosphorylated and involved in inside out signaling phenomena . 1.1430829^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.7394354
The cytoplasmic domains of both CD 4 and CD 8 interact with the protein tyrosine kinase p56lck ( refs 14 17 ) , an essential participant in thymocyte maturation and T cell activation . 0.7394354^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.87604708
First , association of CD 8 with the src kinase p56lck takes place nearly exclusively in rafts , mainly due to increased concentration of both components in this compartment . 0.87604708^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Its extracellular domain is homologous to members of the nerve growth factor receptor super family and its cytoplasmic domain contains a sequence homologous to the binding site for the T cell specific tyrosine kinase p56lck found in the cytoplasmic domains of CD 4 and CD 8 alpha . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Association of p56lck with CD 4 and CD 8 T cell receptor ( TcR ) accessory molecules suggests that p56lck may play a specialized role in antigen induced T cell activation . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Tyrosine phosphorylation has been implicated as important in signaling through each of these pathways , but except for p56lck , a member of the Src family that associates with CD 4 and CD 8 , the protein tyrosine kinases involved have not been defined . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Tyrosine phosphorylation of several intracellular proteins is observed very early during T cell activation . p56lck , a non receptor src like protein tyrosine kinase ( PTK ) which is associated with the intracellular domains of CD 4 and CD 8 co receptors , has been implicated in these early signal transduction events . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
The presence of p56lck associated with CD 4 or CD 8 was also examined in early thymocytes , natural killer cells , and macrophages . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Activated lck tyrosine protein kinase stimulates antigen independent interleukin 2 production in T cells . p56lck , a member of the src family of cytoplasmic tyrosine kinases , is expressed predominantly in T cells where it associates with the T cell surface molecules CD 4 and CD 8 . ^^^ Mutants of CD 4 and CD 8 that have lost the ability to associate with p56lck no longer enhance antigen induced T cell activation . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
During this engagement , CD 8 may deliver TCR independent signals via its associated protein tyrosine kinase , p56lck . ^^^ The bsAb may , therefore , be instrumental in studying T cell signaling pathways , in particular the role of CD 8 associated p56lck therein . . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
The CD 4 and CD 8 antigens on T cells have been shown to associate with the Src family member p56lck and a GTP binding protein , p 32 . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
In helper and cytotoxic T cells it is physically associated with the CD 4 and CD 8 surface antigens and appears to play a role in signal transduction during T cell activation . p56lck contains both an SH 3 and an SH 2 Src homology domain . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Correlation between up regulation of lymphokine mRNA and down regulation of TcR , CD 4 , CD 8 and lck mRNA as shown by the effect of CsA on activated T lymphocytes . ^^^ Full activation of different T cell populations via the TcR / CD3 complex leads to transient expression of lymphokine mRNA and to transient and specific down modulation of TcR , CD 4 , CD 8 and p56lck mRNA ( Multi Receptor Complex or MRC mRNA ) . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
This identity included a glycine residue at position 2 ( present in all Scr related tyrosine protein kinases ) and a cysteine motif at positions 20 and 23 , which allows binding of p56lck to CD 4 and CD 8 in mammalian T lymphocytes . ^^^ Additional studies showed that p56tkl is structurally similar to mammalian p56lck and that it is physically associated with the avian CD 4 and CD 8 T cell surface antigens . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
These results suggest that CD 4 and CD 8 mediate negative regulation of T cell IL 2 responses via cytoplasmically associated p56lck . . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Our data also provide evidence that CD 8 alpha signal transduction through its cytoplasmic tail by association with p56lck is not an absolute requirement for antigen recognition by T cells . . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
CD 8 expressed in splenic T cells associated with the lymphoid specific tyrosine protein kinase p56lck , participated in T cell activation and conferred an increased xenogeneic response to human MHC class 1 Ag . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
The protein tyrosine kinase activity associated with CD 4 and CD 8 ( p56lck ) and the phosphatase activity of CD 45 have been implicated elsewhere in jointly regulating peripheral T cell signal transduction and subsequent cellular responses . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
The association of one of these kinases , p56lck , with the CD 4 and CD 8 cell surface receptors in T lymphocytes has provided a model system through which the first function for a src related tyrosine kinase has been defined . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Lymphocyte specific tyrosine protein kinase p56lck is physically associated with CD 4 and CD 8 T cell surface molecules , suggesting that it may transduce CD4 / CD8 triggered tyrosine phosphorylation signals during antigen stimulation . ^^^ This finding provides direct evidence that p56lck can positively regulate T cell functions and that it mediates at least some of the effects of CD 4 and CD 8 on T cell activation . . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
The association of the protein tyrosine kinase p56lck with CD 4 and CD 8 provides a potential mechanism in the generation of intracellular signals . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
This association may allow CD 45 to engage and dephosphorylate lck or another CD 4 or CD 8 associated substrate in order to reset the receptor complex to receive a new set of stimuli . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Molecular analysis of the interaction of p56lck with the CD 4 and CD 8 antigens . ^^^ Our earlier work demonstrated that the CD 4 and CD 8 receptors are coupled to a protein tyrosine kinase termed p56lck from normal and transformed T lymphocytes . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
We also compare the effects of expressing truncated forms of CD 4 or CD 8 alpha that do not have a cytoplasmic tail and thus do not associate with the T cell specific tyrosine kinase p56lck , which has been implicated in T cell activation . ^^^ Our data further indicate that the cytoplasmic tail and / or the associated p56lck are primarily significant for interleukin 2 secretion by the hybridomas we have examined when CD 4 or CD 8 can bind to the same major histocompatibility complex ligand as the T cell receptor . . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
In this instance , the magnitude of this enhancement is not as great and appears ( at least for CD 8 ) to be independent of the cytoplasmic tail and the associated p56lck . ^^^ The dependence of co receptor function on the cytoplasmic tail of CD 4 or CD 8 may reflect the activity of the associated intracellular tyrosine kinase p56lck . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
The CD 4 and CD 8 T cell surface antigens are physically associated with the tyrosine protein kinase p56lck . ^^^ Accumulating data indicate that p56lck transduces intracellular tyrosine protein phosphorylation signals upon engagement of CD 4 and CD 8 by major histocompatibility complex ( MHC ) determinants expressed on antigen presenting cells ( APCs ) . ^^^ Recent studies show that these p56lck related phosphorylation events enhance T cell receptor ( TCR ) mediated functions and are critical for the proposed co receptor roles of CD 4 and CD 8 . p56lck is also capable of enhancing antigen receptor responsiveness in the absence of CD 4 or CD 8 expression , suggesting that it can directly contribute to the TCR induced tyrosine phosphorylation signal . . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
A novel form of receptor kinase interaction was first described in the interaction between the CD 4 and CD 8 antigens and the protein tyrosine kinase p56lck . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
The ability of IL 2 to induce p56lck activation was found to be independent of the capacity of p56lck to associate with either CD 4 or CD 8 . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Interaction of the unique N terminal region of tyrosine kinase p56lck with cytoplasmic domains of CD 4 and CD 8 is mediated by cysteine motifs . p56lck , a lymphocyte specific member of the src family of cytoplasmic protein tyrosine kinases , is associated noncovalently with the cell surface glycoproteins CD 4 and CD 8 , which are expressed on functionally distinct subpopulations of T cells . ^^^ Using transient coexpression of p56lck with CD 4 or CD 8 alpha in COS 7 cells , we show that the unique N terminal region of p56lck binds to the membrane proximal 10 and 28 cytoplasmic residues of CD 8 alpha and CD 4 , respectively . ^^^ Two cysteine residues in each of the critical sequences in CD 4 , CD 8 alpha , and p56lck are required for association . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
The strong non covalent interactions between T lymphocyte surface CD 4 or CD 8 molecules and the intracellular membrane associated protein tyrosine kinase lck are likely to mediate the role of CD 4 and CD 8 molecules in the immune response . ^^^ Complementary charged regions in the C terminal intracytoplasmic portions of CD 4 or CD 8 , and in the N terminal region of protein tyrosine kinase lck were noted . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Cross linking of T cell surface molecules CD 4 and CD 8 stimulates phosphorylation of the lck tyrosine protein kinase at the autophosphorylation site . p56lck , a lymphocyte specific tyrosine protein kinase , binds to the cytoplasmic tails of the T cell surface molecules CD 4 and CD 8 . ^^^ Cross linking of CD 8 stimulated tyrosine phosphorylation of p56lck strongly in murine L 3 and GA 4 cells , slightly in splenocytes , but not detectably in thymocytes . ^^^ Therefore , the effect of antibody induced aggregation of CD 4 and CD 8 on the tyrosine phosphorylation of p56lck differs , at least quantitatively , from what occurs during antigen induced T cell activation . . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Coclustering CD 45 with CD 4 or CD 8 alters the phosphorylation and kinase activity of p56lck . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Two other T cell integral membrane proteins , CD 4 and CD 8 , are associated with the protein tyrosine kinase ( PTK ) , lck . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
With the exception of p56lck , a tyrosine kinase specific to T lymphoid cells that associates with the T cell transmembrane proteins CD 4 and CD 8 , the kinases that function in these pathways are unknown . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
The CD 4 and CD 8 antigens are coupled to a protein tyrosine kinase ( p56lck ) that phosphorylates the CD 3 complex . ^^^ In this study , we reveal that the human CD 8 antigen is also associated with the T cell specific protein tyrosine kinase ( p56lck ) . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
In mature T cells , CD 4 and CD 8 molecules are each associated with the src like protein tyrosine kinase p 56 lck and signals transduced by TCR and CD 4 activate tyrosine kinases that phosphorylate TCR zeta chains and other intracellular substrates . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Recent evidence suggesting that the cytoplasmic domains of CD 4 and CD 8 are physically , and perhaps functionally , linked to the T cell specific tyrosine protein kinase , p56lck , adds a new dimension to our current understanding of their physiological function . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
In this report , we describe recent work showing that the CD 4 and CD 8 receptors are coupled to a protein tyrosine kinase , p56lck , from T lymphocytes . ^^^ Two dimensional , nonequilibrium gel electrophoresis demonstrated the similarity of p56lck associated with the CD 4 and CD 8 antigens . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Inability of CD 8 alpha ' polypeptides to associate with p56lck correlates with impaired function in vitro and lack of expression in vivo . ^^^ In this study , we show that truncated CD 8 molecules are impaired in their ability to interact with the protein tyrosine kinase , p56lck , and have decreased ability to restore immune responsiveness in vitro . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
We have shown the CD 4 and CD 8 antigens to be associated with the T cell specific protein tyrosine kinase ( p56lck ) . p56lck is a member of a family of protein tyrosine kinases with an established ability to activate and transform mammalian cells . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
The discovery that a member of the src family of tyrosine protein kinases ( p56lck ) is associated with both the CD 4 and CD 8 T lymphocyte surface glycoproteins provides the first clue to understanding the potential physiological functions of this family of proto oncogenes . . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Activators of protein kinase C induce dissociation of CD 4 , but not CD 8 , from p56lck . ^^^ The CD 4 and CD 8 T cell receptor accessory molecules can both be isolated from T lymphocytes in association with p56lck , a membrane associated , cytoplasmic tyrosine protein kinase that is expressed exclusively in lymphoid cells . ^^^ The enzymatic activity of p56lck may therefore be regulated by CD 4 and CD 8 and be important in antigen induced T cell activation . ^^^ In contrast , exposure of cells to TPA did not cause dissociation of p56lck and CD 8 . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Our data demonstrate that p56lck is specifically modulated with either CD 4 or CD 8 following antibody mediated cross linking of these molecules and that a large fraction of the total cellular lck protein can be coimmunoprecipitated with these surface glycoproteins . ^^^ The CD 4 and CD 8 T cell surface antigens are associated with the internal membrane tyrosine protein kinase p56lck . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Intracellular signaling from the T cell receptor ( TCR ) zeta / CD3 complex is likely to be mediated by associated protein tyrosine kinases such as p59fyn ( T ) , ZAP 70 , and the CD 4 : p56lck and CD 8 : p56lck coreceptors . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Thus , lck is associated with CD 4 , CD 8 , and Thy 1 , whereas fyn is associated with the T cell antigen receptor and Thy 1 . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Interactions between the amino terminal domain of p56lck and cytoplasmic domains of CD 4 and CD 8 alpha in yeast . ^^^ The interactions between CD 4 or CD 8 and p56lck were tested using the two hybrid protein interaction system in yeast . ^^^ Plasmid constructs were created which fuse the cytoplasmic domains of either CD 4 or CD 8 alpha to the DNA binding protein LexA , and the unique amino terminal domain of p56lck fused to a transcriptional activation domain . ^^^ Yeast transfectants bearing either CD 4 or CD 8 alpha hybrid proteins in combination with the amino terminal p56lck hybrid protein exhibited increased beta galactosidase activity and growth on leucine deficient medium , indicating interactions between these protein domains . ^^^ Quantitation of reporter activation indicated that the interaction of p56lck with CD 8 alpha is at least 18 fold weaker than the interaction with CD 4 in this assay . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
By contrast , the p56lck , another important T cell tyrosine kinase , is directly linked to CD 4 or CD 8 surface glycoproteins with high stoichiometry . ^^^ The p59fyn may be integrated into the plasma membrane by specific phosphotyrosylproteins and so differ from the p56lck tyrosine kinase , which binds preferentially to the CD 4 and CD 8 transmembrane adhesion glycoproteins . . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
CD 8 beta chain influences CD 8 alpha chain associated Lck kinase activity . ^^^ In the parent hybridoma , cross linking of the CD 8 alpha chain led to minimal enhancement of CD 8 associated Lck tyrosine kinase activity . ^^^ In the CD 8 beta+ transfectants , several observations suggested that CD 8 beta modifies CD 8 alpha associated Lck tyrosine kinase activity : ( a ) in in vitro kinase assays , antibody mediated crosslinking of CD 8 alone , or CD 8 cross linking with the TCR , resulted in 10 fold greater activation of Lck kinase activity , compared to cells expressing CD 8 alpha alpha alone ; ( b ) in vivo , markedly enhanced tyrosine phosphorylation of several intracellular proteins was observed upon CD 8 cross linking with the TCR in CD 8 alpha beta expressing cells , compared to cells expressing CD 8 alpha alpha alone ; and ( c ) Lck association with CD 8 alpha was stabilized by the coexpression of CD 8 beta . ^^^ Thus , the differential Lck kinase activation and tyrosine phosphorylation seen with CD 8 alpha alpha vs . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Defective cytokine expression but adult type T cell receptor , CD 8 , and p56lck modulation in CD 3 or CD 2 activated T cells from neonates . ^^^ The results indicate that in newborn cells , despite a reduced lymphokine expression observed after CD 2 or CD 3 MAb activation , the up regulation of the T cell receptor , CD 8 , and p56lck was similar to that found in adult cells , as was also found after phytohemagglutinin activation of both types of cells . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
CD 8 T cell stimulation with the wild type peptide antigen led to activation of p56lck kinase activity , interleukin 2 secretion , cytotoxicity , and clonal expansion . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
A src like tyrosine kinase , p56lck , is associated with the cytoplasmic domain of both CD 4 and CD 8 and may be involved in transmembrane signaling . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Iron differentially modulates the CD 4 lck and CD 8 lck complexes in resting peripheral blood T lymphocytes . ^^^ We have examined the effect of iron in the form of ferric citrate on the CD 4 lck and CD 8 lck complexes in view of the key role played by the tyrosine kinase p56lck in regulating T cell functions . ^^^ Ferric citrate was seen to differentially modulate the CD 4 lck and CD 8 lck complexes in resting peripheral blood T lymphocytes ( PBLs ) cultured in the presence of this metal salt for periods of 20 to 24 hr . ^^^ Thus , whereas ferric citrate invariably induced a marked decrease in the in vitro activity of the CD 4 associated lck by three to fourfold at 100 microM ( P < 3 10 10 ( 5 ) ) , it did not affect significantly the in vitro activity of the CD 8 associated lck , although modest decreases were observed in some experiments . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Signaling properties of CD 8 have been ascribed to the alpha chain , which binds to both the MHC class 1 and to p56lck , respectively . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
These results suggest that the inhibitory mechanism induced by the CD 8 mAb may prevent the activation of the PI PLC activity , probably through the CD 8 alpha associated protein tyrosine kinase p56lck . . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
The ectodomains of the T cell surface glycoproteins CD 4 and CD 8 bind to membrane proximal domains of MHC class 2 and class 1 molecules , respectively , while both cytoplasmic domains interact with the protein tyrosine kinase ( PTK ) p56lck ( lck ) through a shared cysteine containing motif . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
New insights have been gained in several areas : the avidity regulation of beta 1 and beta 2 integrins and their role in signal transduction ; the regulation of CD 8 avidity ; the role of Lck in CD 4 coreceptor activity ; and the novel role for CD 2 adhesion in the T cell antigen response . . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
The similarity of the relationship between this intramembrane p 75 and / or Ly6C and the cytoplasmic Fgr to the relationships previously reported between T cell antigen receptor complex including CD 4 and CD 8 coreceptors , and Lck or Fyn in T cells and between surface IgM and Lyn or Blk in B cells suggested that the Fgr and p 75 or Ly6C are indeed associated each other and responsible for recognition of extracellular substances ( either cellular or non cellular ) and for signal transduction . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
P56lck A lymphocyte specific protein tyrosine kinase : activation , regulation and signal transduction . p56lck , a src family protein tyrosine kinase interacts with several T cell receptors , like : CD 4 , CD 8 , CD 2 and the beta chain of the IL 2 , thereby receptors devoid of kinase activity may transduce signals via tyr phosphorylation . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
In T lymphocytes , CD 2 forms part of a loosely associated membrane complex which includes the T cell receptor ( TcR ) for antigen , the CD 3 subunits , CD 4 or CD 8 , CD 5 and the protein tyrosine kinases p56lck and p59fyn . ^^^ Using in vitro kinase assays with immune complexes precipitated from cell lysates , we demonstrate that CD 2 can associate with p56lck and p59fyn in a rat thymoma line that does not express CD 4 or CD 8 , and in a TcR negative Jurkat cell line . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
In T cells , several nontransmembrane protein tyrosine kinases are associated with receptors ; for example , Lck is bound to the coreceptors CD 4 and CD 8 and becomes activated upon their stimulation . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
The distinct biological consequences of anti CD 4 and anti CD 8 pretreatment correlate with the differential association of their respective ligands with the cellular protein tyrosine kinase , p56lck . ^^^ While both T cell lineages contain similar levels of cellular p56lck , tenfold more is associated with CD 4 than with CD 8 . ^^^ This difference is associated with the differential effects of pretreatment with anti CD 4 and anti CD 8 on the distribution and activity of p56lck . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
The similarity of relationship between this intramembrane p 70 and / or Ly6C and cytoplasmic Fgr to relationships previously reported between T cell antigen receptor complex , including CD 4 and CD 8 coreceptors , and Lck or Fyn in T cells and between surface IgM and Lyn or Blk in B cells , suggests that the Fgr and p 70 or Ly6C are , indeed , associated with each other and in the murine system may be responsible for recognition of extracellular substances ( either cellular or noncellular ) and for signal transduction in cells of monocyte / macrophage lineage . . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
In addition to an occasional decrease in the amount of CD 8 associated lck , HH patient derived T cells showed a consistent decrease in the relative CD 8 p56lck specific activity . ^^^ Decreased CD 8 p56lck activity in peripheral blood T lymphocytes from patients with hereditary haemochromatosis . ^^^ We were interested in whether CD 4 and CD 8 associated p56lck kinase activities were altered in patients with HH . ^^^ In a study of 18 patients with HH ( with and without low numbers of CD8+ cells ) , the level of autophosphorylation of the CD 8 associated p56lck as well as its phosphotransferase activity , as determined by phosphorylation of an exogenous substrate , was significantly reduced by two to three fold relative to a control population of 23 healthy blood donors ( P < 6 10 10 ( 7 ) . ^^^ CD 8 p56lck activity was decreased in 16 out of 18 patients ( ranging from 1 . 5 to 10 fold decrease ) . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Collectively , the data suggest that , both for T cell induction and the expression of effector function , CD 8 molecules play a decisive role in augmenting TCR mediated signaling , either by promoting TCR contact with antigen or delivering kinases ( p56lck ) to the TCR / CD3 complex , or both . . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
CD 8 has been proposed to function as a co receptor of the TcR to participate in signal transduction , possibly through its cytoplasmic domain that binds to protein tyrosine kinase p56lck . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Signals transduced through the T cell antigen receptor ( TCR ) are modulated by the src family tyrosine kinase p56lck ( lck ) , which associates in mature T cells with the coreceptor molecules CD 4 and CD 8 . ^^^ Importantly , activation of lck in immature CD4+CD8+ thymocytes results from engagement of surface CD 4 molecules , but not surface CD 8 molecules , despite the nearly fourfold greater surface expression of CD 8 than CD 4 . ^^^ The competence of CD 4 to activate lck in CD4+CD8+ thymocytes relates to the fact that a relatively large fraction of surface CD 4 molecules ( 25 50 % ) are associated with intracellular lck molecules , whereas only 2 % of surface CD 8 molecules are associated with lck . ^^^ Thus , the present study demonstrates that ( a ) activation of lck in CD4+CD8+ thymocytes regulates distribution and expression of TCR components ; ( b ) unlike CD 4 molecules , CD 8 molecules on CD4+CD8+ thymocytes can not efficiently activate lck despite their significantly greater surface expression ; and ( c ) the amount of lck associated with CD 4 in the CD4+CD8+ thymocytes is inversely related to the extent of CD 4 engagement by MHC class 2 molecules in the thymus . . ^^^ Regulation of T cell receptor expression in immature CD4+CD8+ thymocytes by p56lck tyrosine kinase : basis for differential signaling by CD 4 and CD 8 in immature thymocytes expressing both coreceptor molecules . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
We discuss the possibility that IL 2 independent growth may be associated with intracellular redistribution of p56lck from CD 8 alpha to IL 2R beta , thus generating constitutively active IL 2R . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
IL 2 stimulation of T lymphocytes induces sequential activation of mitogen activated protein kinases and phosphorylation of p56lck at serine 59 . p56lck , a member of the src family of non receptor protein tyrosine kinases , is expressed almost exclusively in cells of lymphoid origin . p56lck is known to associate with the T lymphocyte surface glycoproteins CD 4 and CD 8 , and plays a critical role in both T lymphocyte development and activation . p56lck also associates with the beta subunit of the IL 2R , and is activated when IL 2 binds to its receptor . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Thus the contribution to cell signalling provided by the co association of p56lck and CD 8 is particularly relevant for CD 8 dependent cells . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
The association between CD 45 and lck does not require CD 4 or CD 8 and is independent of T cell receptor stimulation . ^^^ We show now that CD 45 associates with lck independently of both CD 4 and CD 8 . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
In the double positive transfectants , CD 4 and CD 8 associated with equal amounts of p56lck ( Lck ) , and both molecules enhanced interleukin 2 ( IL 2 ) production equivalently when cross linked with suboptimal levels of anti TCR antibody . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
The cytoplasmic tail of 4 1BB contains the sequence Cys Arg Cys Pro , which is similar to the sequence Cys 10 Cys Pro , which mediates the binding of the CD 4 and CD 8 molecules to the protein tyrosine kinase p56lck . ^^^ Analysis of mutant p56lck recombinant proteins showed that two cysteine residues critical for p56lck CD 4 ( or CD 8 ) complex formation are also required for the p56lck 4 1BB interaction . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
CD 4 and CD 8 contribute to activation signals through their cytoplasmic association with p56lck . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Thymic selection of cytotoxic T cells independent of CD 8 alpha Lck association . ^^^ The biological relevance of CD 8 alpha Lck association in T cell development was tested with transgenic mice generated to express a CD 8 alpha molecule with two amino acid substitutions in its cytoplasmic domain , which abolishes the association of CD 8 alpha with Lck . ^^^ Thus , CD 8 alpha Lck association is not necessary for positive selection , negative selection , or CD 8 dependent cytotoxic function . . ^^^ The CD 8 alpha cytoplasmic domain associates with p56lck , a nonreceptor protein tyrosine kinase . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
The lck gene encodes a lymphocyte specific protein tyrosine kinase of the nonreceptor type that is implicated in signal transduction pathways emanating from the CD 4 and CD 8 coreceptors . ^^^ The protein tyrosine kinase p56lck regulates thymocyte development independently of its interaction with CD 4 and CD 8 coreceptors [ corrected ] . ^^^ Here we report that a mutant form of p56lck , which is incapable of interacting with CD 4 or CD 8 , behaves indistinguishably from association competent p56lck with respect to its ability to affect thymocyte maturation . ^^^ These data support the view that src family kinases perform highly specialized and often nonoverlapping functions in hematopoietic cells , and that p56lck acts independently of its association with CD 4 and CD 8 to regulate thymocyte development . . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
We report here that the human Fc gamma RIIIA is complexed with p56lck , a src family PTK previously found associated with the CD 4 and CD 8 receptors on T cells . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Lck associates specifically with the cytoplasmic domains of both CD 4 and CD 8 T cell surface glycoproteins and interacts with the beta chain of the interleukin 2 receptor , which implicates Lck activity in signal transduction during thymocyte ontogeny and activation of mature T cells . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
The Src related protein tyrosine kinase p56lck is essential for antigen specific signal transduction and thymic maturation of T cells that have an alpha beta T cell receptor ( TCR ) , presumably by physical association with CD 4 or CD 8 molecules . ^^^ To evaluate the requirement for p56lck in the development of T cells that have gamma delta TCRs , which generally do not express CD 4 or CD 8 , p56lck mutant mice were bred with TCR gamma delta transgenic mice . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
In addition to its association with CD 4 and CD 8 , it appears that p56lck is also important during the early stages of thymic development . . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Concomitant to suppression of the kinase by NAD , CD 8 mediated transmembrane signaling and p56lck kinase activation are inhibited . . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
CD 8 is physically associated with the cytoplasmic tyrosine kinase , p56lck and , in addition to increasing the avidity between the TCR and MHC / peptide complex , may transduce intracellular signals . ^^^ This finding demonstrates that , for a TCR MHC system in which the level of CD8alpha is the deciding factor in cell fate , negative selection does not require increased CD 8 dependent signaling through p56lck . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
We demonstrate in vivo in association of CD 8 alpha alpha / p56lck with the tyrosine kinase ZAP 70 after CD 3 stimulation of Jurkat cells . ^^^ A phosphopeptide competing in vitro for the binding of tyrosine phosphorylated proteins to the SH 2 domain of p56lck specifically impedes the association of ZAP 70 with CD 8 alpha alpha / p56lck without affecting the zeta / ZAP 70 interaction . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Disulfide bond formation between the n terminal region of p56LCK and the cytoplasmic domain of CD 8 studied by electrospray ionization and matrix assisted desorption / ionization time of flight mass spectrometry . ^^^ Masses of the complexes formed between the C terminal region of CD 8 alpha ( CD 8 alpha C ) and the N terminal region of p56lck ( p56lckN ) were measured by using Electrospray Ionization ( ESI ) and Matrix Assisted Laser Desorption / Ionization ( MALDI ) Mass Spectrometry ( MS ) . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
T cell receptor and CD 8 dependent tyrosine phosphorylation events in cytotoxic T lymphocytes : activation of p56lck by CD 8 binding to class 1 protein . ^^^ When CD 8 bound to class 1 , phosphorylation of p56lck decreased to below the basal level found in unstimulated cells , accompanied by a substantial increase in kinase activity . ^^^ These results are consistent with the two step model for TCR activation of CD8 / class 1 interactions and directly demonstrate that CD 8 binding to class 1 leads to up regulation of p56lck activity . . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
In both types of CTL clones , p 56 ( lck ) was found associated with the TCR complex , and CD 3 components were found associated with CD 8 before CD 3 engagement . ^^^ Upon CD 3 engagement , ZAP 70 was also found associated with the TCR complex and the kinase activity ( p 56 ( lck ) ) associated with CD 8 increased . ^^^ These data indicate that signals resulting from exclusive CD 3 engagement can influence CD 8 molecular associations and activate CD 8 bound p 56 ( lck ) . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
In more mature thymocytes , p56lck is associated with the cytoplasmic domain of the TCR coreceptors CD 4 and CD 8 , and cross linking of CD 4 leads to p56lck activation . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Interestingly , a small population of TcR gammadelta+ CD 8 alpha alpha+ cells are apparent in lck / fyn / animals , demonstrating that TcR alphabeta+ CD 8 alpha alpha+ and TcR gammadelta+ CD8alpha alpha+ IEL have distinct PTK requirements for their development or expansion . ^^^ Overall , the data presented demonstrate that signals mediated by Lck or Fyn direct TcR alphabeta+ CD8alpha alpha+ IEL maturation but are dispensable for the development of TcR gammadelta+ CD 8 alpha alpha+ IEL . . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Expression of this enzyme was shown to exert regulatory functions on CTL proliferation and cytotoxic activity , presumably by modulating activity of the protein tyrosine kinase p 56 ( lck ) , which is associated with the CTL co receptor CD 8 . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
The cytoplasmic domains of the CD 4 , CD 8 and 4 1BB receptors contain the sequence Cys 10 Cys Pro which is directly involved in coupling to another pair of cysteines in the N terminal domain of the src family tyrosine kinase p 56 ( lck ) . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
We found that in T cells Lck was targeted correctly , independently of the cell surface proteins CD 4 and CD 8 with which it interacts . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Previously , a protein required for T cell transformation by the DNA tumor virus herpesvirus saimiri ( HVS ) and designated tyrosine kinase interacting protein ( Tip 484 ) was shown to interact with and dramatically upregulate the activity of p56lck . p56lck is a nonreceptor tyrosine kinase that is essential for signaling by the T cell receptor and also interacts with the CD 4 , CD 8 , and interleukin 2 receptors . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
T cells activated by IEC are CD8+ , suppressor in function , and dependent upon CD 8 associated p56lck activation . ^^^ The staining patterns correlated with the ability of IEC to activate CD 8 associated p56lck . ^^^ In contrast , both UC and CD IEC activated CD 4 and , to a much lesser extent , CD 8 associated p56lck . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Moreover , we show that the tyrosine kinase p56lck , which is associated with both CD 4 and CD 8 , is activated only in CD 4 ( + ) lymphocytes , making this lectin a good model for the study of cell signaling triggered in this restricted subpopulation . . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
These studies suggest that AEC are capable of stimulating CD 4 and CD 8 associated lck and CD 3 associated fyn kinases through class 2 dependent and independent pathways . . ^^^ To determine whether CD 3 associated p59fyn , or CD 4 and CD 8 associated p56lck phosphotyrosine kinases ( PTK ) were involved , we assayed kinase activity in lymphocyte lysates immunoprecipitated with anti p56lck and p59fyn mAbs . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
All glycosylphospha tidylinositol ( GPI ) anchored proteins ( CD 14 , CD16B , CD 48 , CD 59 , CD 87 and GPI anchored versions of CD 2 and CD 25 ) and protein tyrosine kinase ( PTK ) Lck but not transmembrane proteins ( CD 2 , CD 4 , CD 5 , CD 6 , CD 8 ) and PTK ZAP 70 were in part localized in buoyant , lipid rich , detergent resistant membrane GPI microdomains of the COS cells . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Co capping studies reveal CD8 / TCR interactions after capping CD 8 beta polypeptides and intracellular associations of CD 8 with p 56 ( lck ) . ^^^ In addition , the cytoplasmic domain of CD 8 associates with the intracellular tyrosine kinase p 56 ( lck ) ( lck ) promoting recruitment of lck to the TCR signaling complex . ^^^ In addition , we show by microscopy that intracellular lck redistributes very efficiently to the area of a CD 8 cap , suggesting that there is a stronger association between lck and CD 8 than has been proposed from immunoprecipitation analyses . . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
We present a model of thymocyte differentiation that proposes that the relative balance of signals delivered by TCR engagement and by p56lck activation is responsible for directing commitment to the CD 8 or CD 4 lineages . . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
We show that in contrast to mouse CD4+8+ thymocytes , which express both a complete and a truncated CD 8 alpha chain ( CD 8 alpha ' ) unable to bind p56lck , rat thymocytes only express full length CD 8 alpha molecules . ^^^ Mice , but not rats , therefore may use CD 8 alpha ' as a `` dominant negative ' ' coreceptor chain to attenuate the CD 8 signal , thereby facilitating MHC class 2 recognition through the higher amount of p56lck delivered , and rats may use a different mechanism for MHC class distinction during positive selection . . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Finally , this domain contains a Cys 10 10 Cys sequence that resembles that of p 56 ( lck ) , which is responsible for the interaction with the cytoplasmic tails of CD 4 and CD 8 . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
The cytoplasmic domain of CD 8 beta regulates Lck kinase activation and CD 8 T cell development . ^^^ Previous studies have shown that CD 8 beta plays a role in both enhancing CD 8 alpha associated Lck kinase activity and promoting the development of CD 8 lineage T cells . ^^^ To examine the role of this enhancement in the maturation of CD 8 lineage cells , we assessed CD 8 alpha associated Lck kinase activity in both T cell hybridomas and thymocytes of mice expressing CD 8 beta mutations known to impair CD 8 T cell development . ^^^ Lack of CD 8 beta expression or expression of a cytoplasmic domain deleted CD 8 beta resulted in a severalfold reduction in CD 8 alpha associated Lck kinase activity compared with that observed with cells expressing wild type CD 8 beta chain . ^^^ This analysis indicated a critical role for the cytoplasmic domain of CD 8 beta in the regulation of CD 8 alpha associated Lck activity . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Lck is a lymphoid specific , Src family protein tyrosine kinase that is known to interact with the T cell coreceptors , CD 4 and CD 8 . ^^^ A pair of cysteines on each molecule is essential for association , suggesting that CD 4 or CD 8 may interact with Lck by jointly coordinating a metal ion . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
CD 4 ( + ) CD 8 ( + ) DP cells expressing active p56lck have been shown to lack functional alpha / beta T cell receptor ( TCR ) complexes due to p56lck mediated inhibition of TCR gene Vbeta Dbeta rearrangement . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
In view of the known intracellular signalling mechanism of CD 8 using the tyrosine kinase p 56 lck , we studied p 56 lck expression by Western blot and found lack of consistent expression of the CD 8 surface antigen , with most lacking p 56 lck . ^^^ The CD 8 on the B CLL surface is immunochemically identical to the antigen on T cells , but is not accompanied by its usual signalling mechanism of p 56 lck tyrosine kinase and therefore is unlikely to be a functionally active receptor . . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
This response is not observed in CD 8 T cells and when either the CD 8 : MHC or CD 8 : Lck interactions are prevented . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Expression of a gfi 1 transgene targeted to T cells by the lck proximal promoter provokes a reduction of peripheral CD 4 and CD 8 positive T cells but nevertheless weakly predisposes transgenic animals for the development of T cell lymphoma . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
The data suggest that the tyrosine kinase p56lck ( lck ) plays a crucial role in determining lineage choice , in that maturation of thymocytes into the CD 4 lineage occurs upon recruitment of active lck to the T cell receptor ( TCR ) / CD3 complex , whereas CD 8 maturation can be induced by CD 3 ligation in the absence of co receptor mediated lck recruitment . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
The results demonstrate that low level expression of CD 5 on CD 4 ( ) CD 8 ( ) ( double negative , DN ) thymocytes is independent of TCR gene rearrangement ; however , induction of CD 5 surface expression on DN thymocytes requires engagement of the pre TCR and is dependent upon the activity of p 56 ( lck ) . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
However , p 56 ( lck ) , which is expressed in T lymphocytes , has not been found in macrophages , suggesting that other src family kinases may be involved in AM and MC CD 8 signaling . . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
The Src related tyrosine kinase p 56 ( lck ) ( Lck ) is primarily expressed in T lymphocytes where it localizes to the cytosolic side of the plasma membrane and associates with the T cell coreceptors CD 4 and CD 8 . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
The results of our studies showed that in T cells , CD 45 AP is part of a multimolecular complex that includes not only CD 45 , but also TCR , the CD 4 and CD 8 coreceptors , and p 56 ( lck ) . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Developmental regulation of Lck targeting to the CD 8 coreceptor controls signaling in naive and memory T cells . ^^^ In naive cytotoxic T cells , only a few CD 8 molecules are associated with Lck and the kinase is homogeneously distributed inside the cell . ^^^ However , in vivo priming of naive T cells induces the targeting of Lck to the CD 8 coreceptor in the cell membrane and the consequent organization of a more efficient TCR signaling machinery in effector and memory cells . . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Finally , overexpression of ITM2A under control of the lck proximal promoter in mice results in partial downregulation of CD 8 in CD 4 ( + ) CD 8 ( + ) double positive ( DP ) thymocytes , and a corresponding increase in the number of CD 4 ( + ) CD 8 ( lo ) thymocytes . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Previous studies have shown that normal intestinal epithelial cells ( IECs ) are able to selectively activate CD 8 ( + ) T cells with suppressor activity , inducing proliferation associated with the activation of both the CD 8 associated kinase p 56 ( lck ) and the T cell receptor ( TCR ) associated kinase p 59 ( fyn ) . ^^^ This process appears to relate in part to a 180 kDa IEC surface glycoprotein , gp 180 , which binds to CD 8 and activates CD 8 associated p 56 ( lck ) . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
The coreceptors CD 8 and CD 4 may contribute to these different outcomes through interactions with class 1 and class 2 MHC , respectively , and through interactions with the tyrosine kinase p56lck ( Lck ) via their cytoplasmic domains . ^^^ In this paper we provide evidence that an alternatively spliced form of CD 8 that can not interact with Lck ( CD 8 alpha ' ) can influence the CD 4 vs CD 8 lineage decision . ^^^ These results indicate that CD 8 alpha ' favors the development of CD 8 lineage T cells , perhaps by reducing Lck activity upon class 1 MHC recognition in the thymus . . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Here we show that LAT associates with surface CD 4 and CD 8 coreceptors and that its association is promoted by the same coreceptor cysteine motif that mediates Lck binding . ^^^ In fact , LAT competes with Lck for binding to individual coreceptor molecules but differs from Lck in its preferential association with CD 8 rather than CD 4 in CD 4 ( + ) CD 8 ( + ) thymocytes . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
We show that p 56 ( lck ) transcripts are detectable in each CD 4 ( ) CD 8 ( ) double negative ( DN ) thymocyte subset defined by CD 25 and CD 44 . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
The function of the CD 8 alpha chain for binding to MHC class 1 and associating with the tyrosine kinase p56lck was demonstrated with CD 8 alpha alpha homodimers . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Maturational changes at the CD 4 ( ) CD 8 ( ) double negative ( DN ) to CD 4 ( + ) CD 8 ( + ) double positive ( DP ) transition are dependent on signals generated via the pre T cell receptor ( TCR ) and the nonreceptor protein tyrosine kinase p 56 ( lck ) ( Lck ) . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Although the transmembrane region is well conserved in all CD 8 alpha sequences analyzed to date , the putative trout cytoplasmic region differs and , in fact , lacks the consensus p56lck motif common to other CD 8 alpha sequences . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
The early CD 3 induced protein tyrosine phosphorylations are conserved in both CD 4 ( + ) and CD 8 ( + ) subsets , and the levels of the T cell protein tyrosine kinases p 56 ( Lck ) , p 59 ( Fyn ) and ZAP 70 are normal . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Since the tyrosine kinase Lck binds with more affinity to CD 4 than CD 8 , it has been proposed as a candidate to mediate this process . ^^^ By using transgenic mice with altered Lck activity , we show that thymocytes carrying a class 2 restricted TCR develop into functional CD 8 T cells when Lck activity is reduced . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Here we present the characterization of a thymoma from an Lck ( / ) mouse , where the block in thymocyte development is overcome and the transition between the CD 4 ( ) CD 8 ( ) and CD 4 ( + ) CD 8 ( + ) stages is fully restored . ^^^ Hence ZAP 70 overexpression in thymocytes is associated with recovery from the developmental arrest caused by the absence of Lck , suggesting a role for ZAP 70 downstream of Lck in the maturation of CD 4 ( + ) CD 8 ( + ) thymocytes . . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
We now demonstrate that the CD8beta extracellular domain increases the avidity of CD 8 binding to MHC 1 , and that the intracellular domain of CD8beta enhances association with two intracellular molecules required for TCR signal transduction , Lck and LAT . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Positive and negative selection of thymocytes depends on Lck interaction with the CD 4 and CD 8 coreceptors . ^^^ In this report , we establish that early events in T lymphopoiesis are restored to Lck deficient mice by provision of a transgene encoding a version of Lck that can not interact with the coreceptors CD 4 and CD 8 . ^^^ In addition , we demonstrate that later events in thymocyte development , specifically , the processes of positive and negative selection , are compromised in mice where the only Lck available can not associate with either CD 4 or CD 8 . ^^^ We conclude that not only is Lck activity required for positive and negative selection , but that that activity must be coupled to the CD 4 and CD 8 coreceptors . . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Activated p56lck directs maturation of both CD 4 and CD 8 single positive thymocytes . p 56 ( lck ) is a protein tyrosine kinase expressed throughout T cell development . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
In both subsets , CD 8 acted as a co stimulatory molecule in vitro and was found to bind the kinase lck efficiently . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Here we show that endogenous Bcl 2 expression is substantially reduced in IRF 1 ( / ) CD8+ thymocytes and that introduction of a human Bcl 2 transgene driven by Emu or lck promoter in IRF 1 ( / ) mice restores the CD 8 ( + ) T cell development . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
These events are prevented by blocking Src kinases or CD 8 binding to TCR associated Kd molecules , indicating that CTL activation is initiated by cross linking of CD 8 associated p56lck . ^^^ Because efficient association of CD 8 and p56lck takes place in rafts , both reagents , though in different ways , impair coupling of p56lck to TCR , thereby inhibiting the initial and essential activation of p56lck induced by cross linking of engaged TCR . . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Differential association of CD 45 isoforms with CD 4 and CD 8 regulates the actions of specific pools of p56lck tyrosine kinase in T cell antigen receptor signal transduction . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
CD8beta endows CD 8 with efficient coreceptor function by coupling T cell receptor / CD3 to raft associated CD8 / p56 ( lck ) complexes . ^^^ We find that the cytoplasmic portion of CD8beta , mainly due to its palmitoylation , mediates partitioning of CD 8 in lipid rafts , where it efficiently associates with p 56 ( lck ) . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
We compared the phenotype and signaling capacity of Lck deficient T cells with T cells from mice expressing a dominant inhibitory form of Lck and found that both mouse strains have diminished numbers of mature CD 8 ( + ) T cells and respond poorly to CD 28 costimulation . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
It has been previously shown that monoclonal antibodies against gp 180 inhibited the activation of CD 8 associated p 56 ( lck ) in T cells . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Transformation of cells by src like kinases leads to altered cell morphology associated with the disassembly of focal contacts and concomitant increase in tyrosine phosphorylation of pp 125 ( FAK ) 10 p 56 ( lck ) is a lymphocyte specific member of the src family of protein tyrosine kinases that associates with cell surface glycoproteins such as CD 4 and CD 8 . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Using either in situ tetramer staining or green fluorescent protein labeled virus specific T cells , we have shown that a single CD 8 ( + ) T cell can engage two or three targets , a synapse occurs at the site of engagement and molecules involved in attachment ( lymphocyte function associated antigen 1 ) , signaling ( Lck ) and lytic activity ( perforin ) are differentially positioned on the T cell . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Examination of lipid rafts isolated by sucrose density gradient centrifugation revealed the constitutive expression of p ( 56 ) Lck in the raft fractions of unstimulated CD 8 ( + ) T cells , whereas p ( 56 ) Lck was recruited to the raft fraction of CD 4 ( + ) T cells only after stimulation with CD3 / 28 beads . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Although the role of CD 8 as a supplier of lck is unchallenged , its role in contributing to the formation of a stable complex between class 1 molecules and the TCR , as well as its role as an adhesion molecule , is less clear . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Even in the absence of direct CD 8 binding , stimulation of AHIII 12 . 2 T cells with `` CD 8 independent ' ' p1049 / A2 produces p 56 ( lck ) activation and calcium flux . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
The CD 8 coreceptor enhances T cell function by stabilizing the TCR / peptide / MHC complex and / or increasing T cell avidity via interactions with the intracellular kinases Lck and LAT . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
To better evaluate the role of hSLAM in MV pathogenesis and MV induced immunosuppression , we created transgenic ( tg ) mice that expressed the hSLAM molecule under the control of the lck proximal promoter . hSLAM was expressed on CD 4 ( + ) and CD 8 ( + ) T cells in the blood and spleen and also on CD 4 ( + ) , CD 8 ( + ) , CD 4 ( + ) CD 8 ( + ) , and CD 4 ( ) CD 8 ( ) thymocytes . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Positive selection of CD 4 single positive ( SP ) cells is also critically dependent on the expression of Lck but not Fyn , while differentiation of CD 8 SP cells proceeds relatively efficiently in the absence of Lck . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
These unpublished data indicate that sHLA I / CD8 ligation elicits the phosphorylation of p56lck protein thyrosin kinase ( PTK ) associated with CD 8 cytoplasmic domain in the absence of any other TCR derived signal , the activation of syk like ZAP 70 PTK and protein kinase C , and extracellular calcium influx . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
A zinc clasp structure tethers Lck to T cell coreceptors CD 4 and CD 8 . ^^^ The T cell coreceptors CD 4 and CD 8 both associate via their cytoplasmic tails with the N terminus of the Src family tyrosine kinase Lck . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Our objective was to examine p 56 ( lck ) protein levels , flow cytometric markers of T cell development ( CD 4 , CD 8 , TCRalphabeta , TCRgammadelta and CD 90 ) and absolute cell numbers in thymus , spleen and blood of zinc deficient ( ZD ) , diet restricted ( DR ) and control ( CTL ) rats . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
There was a marked reduction , particular in advanced stage MM , in the proportion of CD 4 and CD 8 cells expressing CD 28 , CD 152 , CD3zeta , p56lck , ZAP 70 and PI 3 k . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
They hypothesize that the key event in T cell activation is the formation of multimolecular complexes consisting of the TCR and several other polypeptides , including CD 4 or CD 8 , CD 2 , CD 5 and the associated tyrosine kinases p 59 ( fyn ) and p 56 ( lck ) . . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Thymic and splenic cell numbers as well as CD4 / CD8 cellularity were normal in lck FLIP ( S ) transgenic animals , which in contrast to CD 95 deficient mice do not accumulate Thy 1 ( + ) B 220 ( + ) CD 4 ( ) CD 8 ( ) peripheral T cells . c FLIP ( S ) overexpression leads to a significant decrease in activation induced T cell proliferation in vitro . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
CD 4 or CD 8 , are not necessarily competitive with Lck binding to HIV 1 Nef . . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
This finding indicates that the interaction between the intracellular regions of a TCR CD 3 complex recognizing its cognate peptide major histocompatibility complex ( MHC ) antigen , and CD 8 ( plus the kinase Lck ) , is enhanced by a noncognate CD 8 MHC interaction . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Lymphocyte specific protein tyrosine kinase ( LCK ) is a lymphoid specific , Src family protein tyrosine kinase that is known to play a pivotal role in T cell activation and interact with the T cell coreceptors , CD 4 and CD 8 . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Apoptosis of antigen specific T lymphocytes upon the engagement of CD 8 by soluble HLA class 1 molecules is Fas ligand / Fas mediated : evidence for the involvement of p56lck , calcium calmodulin kinase 2 , and Calcium independent protein kinase C signaling pathways and for NF kappaB and NF AT nuclear translocation . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Surface expression levels of CD 4 and CD 8 on transfected CD3+ gated PBMCs were correlated with intracellular Lck levels . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
The paradigm to explain antigen dependent T cell receptor ( TCR ) signaling is based on the activation of the CD 4 or CD 8 coreceptor associated kinase Lck . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Lck associates through its distinctive amino terminal segment with the cytoplasmic tails of either T cell co receptor , CD 4 or CD 8 alpha . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Current data suggest that CD8beta mediated response enhancement proceeds through mechanisms similar to those mediated by CD8alpha , i . e . , interacting with MHC class 1 and stabilizing CD 8 associated Lck activity . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
To determine whether CD 3 ( ) CD8alpha alpha+ IEL represent precursors of T cells developing extrathymically , we examined IEL from knockout mice lacking the recombination activating gene 1 ( rag 1 ) , CD3epsilon , or both Lck and Fyn , in which thymic T cell development is arrested . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Zinc is essential for binding of p 56 ( lck ) to CD 4 and CD8alpha . ^^^ Binding of the protein tyrosine kinase p 56 ( lck ) to T cell co receptors CD 4 and CD8alpha is necessary for T lymphocyte development and activation . ^^^ In an in vitro binding reaction , Zn2+ mediates p 56 ( lck ) association with a glutathione S transferase ( GST ) fusion protein containing the cytosolic domains of CD 4 or CD8alpha ; no other metals tested support binding . ^^^ Furthermore , we show that , within live cells , CD4 . p56 ( lck ) and CD8alpha . p56 ( lck ) interactions occur in a zinc dependent fashion . ^^^ Specifically , pretreatment of the human Jurkat T cell line with membrane permeable zinc chelators disrupts CD4 . p56 ( lck ) complexes , and treatment of COS cells co expressing CD8alpha and p 56 ( lck ) with such chelators likewise leads to dissociation of CD8alpha . p56 ( lck ) complexes . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
Assembly of the CD8alpha / p56 ( lck ) protein complex in stably expressing rat epithelial cells . ^^^ We now describe the biosynthesis , post translational maturation and hetero oligomeric assembly of the human CD8alpha / p56 ( lck ) protein complex in stable transfectants obtained from the same cell line . ^^^ There were no differences in the myristilation of p 56 ( lck ) , or in the dimerization , O glycosylation and transport to the plasma membrane of CD8alpha , between cells expressing either one or both proteins . ^^^ In the doubly expressing cells , dimeric forms of CD8alpha established hetero oligomeric complexes with p 56 ( lck ) , as revealed by co immunoprecipitation assays performed with anti CD8alpha antibody . ^^^ The present study shows that : ( 1 ) the newly synthesized p 56 ( lck ) binds rapidly to CD8alpha and most of the p 56 ( lck ) is bound to CD8alpha at steady state ; ( 2 ) CD8alpha / p56 ( lck ) protein complexes are formed at internal membranes as well as at the plasma membrane ; and ( 3 ) about 50 % of complexed p 56 ( lck ) reaches the cell surface . . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
The introduction of a mutated cysteine motif in CD8alpha , which prevents its binding to LCK and linker for activation of T cells , did not adversely affect expression and T cell cytotoxicity , but counteracted the CD8alpha mediated down regulation of IL 4 and IL 5 , but not IL 10 . ^^^ In conclusion , CD8alpha down regulates the production of major Th 2 type cytokines , in part mediated by LCK and / or linker for activation of T cells , and may induce differentiation of tumor specific Th 1 cells , which makes this coreceptor an interesting candidate to improve the clinical potential of TCR gene transfer to treat cancer . . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
When TX 100 extracts from Jurkat cells were subjected to centrifugation to equilibrium in sucrose gradients we found MAL exclusively in the floating fractions , together with molecules characteristic of the T cell insoluble complexes , such as the tyrosine kinase p56lck , the glycosylphosphatidylinositol anchored protein CD 59 and the ganglioside GM 1 . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
In addition , both MAL and the Src like kinase Lck were identified in GEM obtained from an endosomal enriched membrane fraction . ^^^
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01732 and P06239	Pubmed	SVM Score :0.0
NA