| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| Protein tyrosine kinases p 56 ( Lck ) , SYK , and ZAP 70 and downstream adaptors LAT and SLP 76 have been implicated as essential components in T cell activation . ^^^ |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| LCK , LAT , and the TCR all colocalized with lipid patches , although TCR association was sensitive to nonionic detergent . ^^^ Thus , our results indicate a mechanism whereby TCR engagement promotes aggregation of lipid rafts , which facilitates colocalization of LCK , LAT , and the TCR whilst excluding CD 45 , thereby triggering protein tyrosine phosphorylation . . ^^^ |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| Here we show that LAT associates with surface CD 4 and CD 8 coreceptors and that its association is promoted by the same coreceptor cysteine motif that mediates Lck binding . ^^^ In fact , LAT competes with Lck for binding to individual coreceptor molecules but differs from Lck in its preferential association with CD 8 rather than CD 4 in CD 4 ( + ) CD 8 ( + ) thymocytes . ^^^ |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| The targets for the unresponsive state appear to be diminished Lck activation and absent ZAP 70 and LAT ( linker for activation of T cells ) phosphorylation . ^^^ |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| In Jurkat T cells , 2 bromopalmitate blocked localization of the endogenous palmitoylated proteins Fyn , Lck , and LAT to DRMs . ^^^ |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| First , the inositide binding pocket of the Itk pleckstrin homology domain directs the constitutive association of Itk with buoyant membranes that are the primary site of TCR activation and are enriched in both Lck and LAT . ^^^ |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| In T lymphocytes , several signaling proteins are associated with lipid rafts including the protein tyrosine kinase LCK and the adapter protein LAT . ^^^ |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| We now demonstrate that the CD8beta extracellular domain increases the avidity of CD 8 binding to MHC 1 , and that the intracellular domain of CD8beta enhances association with two intracellular molecules required for TCR signal transduction , Lck and LAT . ^^^ |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| The defect in ERK activation was associated with impaired recruitment of the activated tyrosine kinases Lck and ZAP 70 , phosphorylated forms of the TCR component CD3zeta and the adaptor protein LAT to detergent insoluble glycolipid enriched microdomains ( DIGs ) . ^^^ |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| About 50 % of the T cells from young mice that formed conjugates with peptide pulsed APC were found to form complexes , at the site of binding to the APC , containing CD3epsilon , linker for activation of T cells ( LAT ) , and Zap 70 in a central area and c Cbl , p 95 ( vav ) , Grb 2 , PLC gamma , Fyn , and Lck distributed more uniformly across the interface area . ^^^ |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| As compared to peripheral blood ( PB ) T lymphocytes , SF T cells from some ( but not all ) of the patients analyzed expressed lower levels of TCRalphabeta , CD3epsilon , TCRzeta , p 56 ( lck ) and LAT , while p 59 ( fyn ) , phospholipase C gamma 1 and ZAP 70 expression was unaltered . ^^^ |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| T cell specific signaling molecules Lck , Zap 70 , and LAT play roles in the regulation of HPK 1 during TCR signaling . ^^^ |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| Whereas the inducible tyrosine phosphorylation of TCR zeta and ZAP 70 and the kinase activity of Lck were intact , phosphorylated LAT was rapidly dephosphorylated by raft targeting of activated SHP 1 , leading to defects in LAT activation and subsequent downstream signaling events . ^^^ |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| Notably , palmitate labeled Src family kinase Lck and the linker for activation of T cells ( LAT ) were both displaced from lipid rafts indicating that acylation by PUFAs is not required for protein displacement from rafts in PUFA treated T cells . ^^^ |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| While CD 2 induced p 62 ( dok ) tyrosine phosphorylation was independent of both the p36 / 38 membrane adapter protein linker of activated T cells ( LAT ) and the ZAP70 / Syk family of kinases , it was dependent upon the Src family of kinases including Lck and Fyn . ^^^ |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| The outer membrane leaflet of these peculiar membrane domains contains glycosylphosphatidylinositol anchored proteins , while the inner leaflet contains proteins implicated in signalling , such as the acylated protein kinase p 56 ( lck ) and the palmitoylated adaptator LAT ( linker for activation of T cells ) . ^^^ |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| Confocal fluorescence microscopy shows that Lck colocalizes in rafts with GPI linked proteins , the adaptor protein LAT and Ras , but not with non raft membrane proteins including the protein tyrosine phosphatase CD 45 . ^^^ Our results indicate a mechanism whereby TCR engagement promotes aggregation of lipid rafts , which facilitates colocalization of signaling proteins including Lck , LAT , and the TCR , while excluding CD 45 , thereby potentiating protein tyrosine phosphorylation and downstream signaling . ^^^ |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| We confirmed the presence of adaptor molecules ( LAT ) and Src kinases ( Lck ) in the DRM containing fractions 4 to 6 . . ^^^ |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| The active participation of p 56 ( lck ) , ZAP 70 , p 36 ( LAT ) , and calcium in the bpV dependent signaling cascade leading to COX 2 transcriptional activation was demonstrated using deficient cell lines and specific inhibitors . ^^^ |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| Selective interaction of LAT ( linker of activated T cells ) with the open active form of Lck in lipid rafts reveals a new mechanism for the regulation of Lck in T cells . ^^^ Increased tyrosine phosphorylation of Lck was restored in LAT reconstituted J . ^^^ CaM2 cells suggesting that LAT negatively regulates Lck activity in lipid rafts . ^^^ Co immunoprecipitation experiments from Tyr ( 505 ) > Phe / V5 His expressing cells revealed that LAT preferentially interacts with the ' open ' form of Lck in T cell raft domains . ^^^ These results demonstrate that , unlike the non raft pool , Lck in lipid rafts has a ' closed ' inactive structure , and that LAT plays a role in maintaining this conformation , possibly by facilitating critical associations within lipid rafts via its capacity to interact with the ' open ' form of the kinase . . ^^^ |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| The CD 8 coreceptor enhances T cell function by stabilizing the TCR / peptide / MHC complex and / or increasing T cell avidity via interactions with the intracellular kinases Lck and LAT . ^^^ |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| Anti CD 4 was found to perturb proximal signalling events upon TCR / CD3 ligation , resulting in reduced tyrosine phosphorylation of Zap 70 and LAT ( linker for activation of T cells ) and reduced association of tyrosine phosphorylated LAT with lck . ^^^ |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| Transferrin receptor and the CD 45 tyrosine phosphatase did not colocalise with CT B GM 1 patches , whereas the tyrosine kinase Lck , the scaffolding protein LAT , and endogenous Ras proteins did partially colocalise with CT B GM 1 patches as did transfected EGFP K Ras ( 4B ) and EGFP H Ras . ^^^ |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| Proteins in the lipid raft and nonraft fractions were analyzed by Western blotting and probed for phosphotyrosine activity and for LCK , LAT , and CD 3 epsilon . ^^^ |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| TCR zeta , p 56 ( lck ) and LAT ) but not all ( e . g . ^^^ Oxidative stress exposed proteins were differentially susceptible to proteasomal degradation : whereas modified TCR zeta was relatively resistant , reactive oxygen species ( ROS ) altered LAT and p 56 ( lck ) were much more susceptible . ^^^ |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| Production of LAT but not p56lck was diminished in CD 4 CD8 DN cells from Ly 6A . 2 dysregulated mice when compared with control littermates . ^^^ |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| Although rafts purified from unstimulated cells demonstrated abundant Lck but inabundant LAT , rafts from stimulated cells include an abundance of both components . ^^^ |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| Our data further indicate that following CD 38 engagement , LAT and Lck are tyrosine phosphorylated exclusively in Brij 98 resistant rafts , and some key signaling components translocate into rafts ( i . e . ^^^ Taken together , these results suggest that , unlike the non raft pool , CD 38 in rafts is able to initiate and propagate several activating signaling pathways , possibly by facilitating critical associations within other raft subsets , for example , LAT rafts via its capacity to interact with Lck and CD 3 zeta . ^^^ |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| We also showed that CD 26 cross linking induced less phosphorylation of T cell receptor signaling molecules , lymphoid T cell protein tyrosine kinase ( Lck ) , zeta associated protein 70 ( ZAP 70 ) , T cell receptor zeta ( TCRzeta ) , and linker for activator of T cells ( LAT ) in CBTCs than in PBTCs . ^^^ |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| Here we demonstrate that E2A is required for the complete arrest in both differentiation and proliferation observed in thymocytes with defects in proteins that mediate pre TCR signaling , including LAT , Lck and Fyn . ^^^ |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| We measured the lipid rafts coalescence , the p 56 ( Lck ) and linker of activated T cell ( LAT ) signaling molecules recruitment and activation , the cholesterol content and fluidity in T cell membrane after different methyl beta cyclodextrin ( MBCD ) treatments . ^^^ Our results show that high concentration of MBCD ( 10 mM ) completely disorganized the lipid rafts in T cell membranes of young and elderly donors , however , T cells from elderly donors were less sensitive than T cells of young donors to low concentration of MBCD ( 0 . 5 mM ) . p 56 ( Lck ) and LAT recruitment and activation were affected in T cells of both aged groups . ^^^ |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| We show here that cross linking of PrPc with specific antibodies leads to 1 ) PrPc capping in Jurkat and human peripheral blood T cells ; 2 ) to cocapping with the intracellular lipid raft proteins reggie 1 and reggie 2 ; 3 ) to signal transduction as seen by MAP kinase phosphorylation and an elevation of the intracellular Ca2+ concentration ; 4 ) to the recruitment of Thy 1 , TCR / CD3 , fyn , lck and LAT into the cap along with local tyrosine phosphorylation and F actin polymerization , and later , internalization of PrPc together with the reggies into limp 2 positive lysosomes . ^^^ |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| Crosslinking of purified wild type nave CD 4 cells with anti CD 3 activated Lck and initiated the signaling cascade downstream of Lck , including phosphorylation of ZAP 70 , LAT , and PLC gamma 1 ; calcium flux ; and dephosphorylation and nuclear translocation of the nuclear factor of activated T cells ( NFAT ) p . ^^^ |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| To dissect the early signalling events leading to TNF alpha gene expression , we established that CD 45 crosslinking resulted in tyrosine phosphorylation of p56lck , ZAP 70 , CD 3 zeta , LAT and Vav . ^^^ |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| Importantly , the membrane translocation of Vav 3 was abrogated in Lck , ZAP 70 , LAT , and SLP 76 deficient T cells , where Vav 3 binding to SLP 76 was disrupted . ^^^ |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| We show that the coreceptor CD 2 , the adaptor protein LAT , and tyrosine kinase Lck cocluster in discrete microdomains in the plasma membrane of signaling T cells . ^^^ Using a two color imaging approach that allows tracking of single molecules relative to the CD2 / LAT / Lck clusters , we demonstrate that these microdomains exclude and limit the free diffusion of molecules in the membrane but also can trap and immobilize specific proteins . ^^^ |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| In contrast , the expression of LAT , which like Lck distributes to raft and non raft membrane , or Csk , a kinase with a structure similar to Lck , is not affected by cell aggregation . ^^^ |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| There was a direct correlation between the activation of p 56 ( Lck ) and LAT and their association / recruitment with the lipid raft fractions of CD4+ and CD8+ T cells . p56Lck , LAT and Akt / PKB were weakly phosphorylated in lipid rafts of stimulated CD4+ T cells of elderly as compared to young donors . ^^^ |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| It contains not only the raft markers GM 1 and flotillin , but also some T cell receptor ( TCR ) signalling molecules , including Lck , Fyn and LAT . ^^^ |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| The MM localisation of Lck and LAT was not significantly reduced in PDMP treated cells . ^^^ |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| Biochemically , these traits were associated with higher basal phosphorylation of the TCR signaling proteins ZAP 70 , Lck , and LAT . ^^^ |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| Moreover , other elements of the pre TCR / TCR signaling pathway , like LAT and Lck , were also found in B ALL cells . ^^^ |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| Here we demonstrate that defects in cluster assembly and persistence are associated with defects in T cell activation in the absence of Lck , ZAP 70 , or LAT . ^^^ |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P06239 |
Pubmed |
SVM Score :0.0 |
| NA |
|