| Interacting proteins: P05783 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P05783 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P05783 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P05783 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P05783 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P05783 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P05783 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P05783 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P05783 and P42574 |
Pubmed |
SVM Score :0.0 |
| Immunohistochemistry was performed using antibodies directed against cleaved cytokeratin 18 and active caspase 3 . ^^^ |
|
| Interacting proteins: P05783 and P42574 |
Pubmed |
SVM Score :0.0 |
| Apoptosis was monitored by determining cytokeratin 18 cleavage and caspase 3 activation . ^^^ |
|
| Interacting proteins: P05783 and P42574 |
Pubmed |
SVM Score :0.0 |
| Apoptosis was assessed by immunohistochemistry of active caspase 3 and cytokeratin 18 in liver tissue . ^^^ |
|
| Interacting proteins: P05783 and P42574 |
Pubmed |
SVM Score :0.0 |
| We also found that fresh CD 4 ( + ) cells from these patients showed high levels of both caspase 3 ( CPP 32 ) and its molecular targets , namely PARP and CK 18 . ^^^ |
|
| Interacting proteins: P05783 and P42574 |
Pubmed |
SVM Score :0.0 |
| Apoptotic cells were quantified and apoptotic indices were calculated by computer assisted image analysis following identification of apoptotic cells by morphological analysis , the TUNEL assay , activated caspase 3 and cleaved CK 18 immunohistochemistry . ^^^ An excellent correlation ( R = 0 . 89 ) between the apoptotic indices obtained using activated caspase 3 and cleaved CK 18 immunostaining was observed . ^^^ |
|
| Interacting proteins: P05783 and P42574 |
Pubmed |
SVM Score :0.0 |
| The techniques included light microscopy ( LM ) and transmission electron microscopy ( TEM ) observation of epoxy resin embedded tissue , scanning electron microscopy ( SEM ) , TUNEL assay , and antibodies directed against caspase cleavage products of caspase 3 , cytokeratin 18 ( CK 18 ) , and apoptotic single strand DNA ( ssDNA ) . ^^^ |
|
| Interacting proteins: P05783 and P42574 |
Pubmed |
SVM Score :0.0 |
| Cleavage of CK 18 protein by caspase 3 is a marker of early apoptosis in epithelial cells . ^^^ To investigate the antigen binding characteristics of IgG autoantibodies to CK 18 protein in nonallergic asthma , the bindings of IgG autoantibodies to the fragments of CK 18 protein cleaved by caspase 3 were analyzed by Western blot using serum samples from three patients with nonallergic asthma . ^^^ Recombinant human CK 18 protein was treated by caspase 3 and cleaved into N terminal fragment ( 1 397 amino acids ) and C terminal fragment ( 398 430 amino acids ) . ^^^ |
|
| Interacting proteins: P05783 and P42574 |
Pubmed |
SVM Score :0.0 |
| Similarly , in untransfected breast HBL 100 and lung A 549 epithelial cells , TRAIL induced the formation of cytoplasmic inclusions that contained cleaved cytokeratin 18 and colocalized with active endogenous caspase 3 . ^^^ |
|
| Interacting proteins: P05783 and P42574 |
Pubmed |
SVM Score :0.0 |
| Apoptosis was evaluated by conventional light microscopy , laser scanning confocal microscopy using the nuclear staining dye Hoechst 33342 , immunohistochemical staining for active caspase 3 , and immunohistochemical staining for cytokeratin 18 . ^^^ |
|
| Interacting proteins: P05783 and P42574 |
Pubmed |
SVM Score :0.0 |
| Early markers of apoptosis of cultured cells included lactate dehydrogenase retention in dying cells , cytokeratin 18 cleavage , and caspase 3 activation . ^^^ |
|
| Interacting proteins: P05783 and P42574 |
Pubmed |
SVM Score :0.0 |
| M 30 Mab recognizes a caspase 3 directed cleavage event within cytokeratin 18 , a protein widely distributed in epithelial cells , of which trophoblast cells are classified . ^^^ |
|
| Interacting proteins: P05783 and P42574 |
Pubmed |
SVM Score :0.0 |
| The apoptosis was characterized by chromatin condensation , transferase mediated dUTP nick end labeling ( TUNEL ) staining , proteolytic cleavage of poly ( ADP ribose ) polymerase ( PARP ) and cytokeratin 18 , and activation of caspase 3 . ^^^ |
|
| Interacting proteins: P05783 and P42574 |
Pubmed |
SVM Score :0.0 |
| Trophoblast turnover including apoptosis and necrosis were assessed by histology , immunolocalization of Mib 1 ( proliferation marker ) , Bcl 2 ( apoptosis inhibitor ) , activated caspase 3 ( apoptosis promoter ) , cytokeratin 18 neo epitope formation ( M 30 antibody ) , TUNEL test ( DNA degradation ) , and ( 3 ) H cytidine and ( 3 ) H uridine incorporations . ^^^ |
|
| Interacting proteins: P05783 and P42574 |
Pubmed |
SVM Score :0.0 |
| It was found that zinc concentrations above 100 microM were toxic to Hep 2 cells , inducing cell death in the interval of 96 h as determined by videomicroscopy , selective nuclear staining , and immunofluorescence detection of caspase 3 and specific cytokeratin 18 fragment . ^^^ |
|
| Interacting proteins: P05783 and P42574 |
Pubmed |
SVM Score :0.0 |
| Orthotopic A 549 rat lung tissue sections from drug treated rats and A 549 cell culture responses to exisulind and docetaxel were compared using multiple apoptosis and proliferation analyses [ i . e . , terminal deoxynucleotidyl transferase mediated nick end labeling , active caspase 3 , the caspase cleavage products cytokeratin 18 and p 85 poly ( ADP ribose ) polymerase , and Ki 67 ] . ^^^ |
|
| Interacting proteins: P05783 and P42574 |
Pubmed |
SVM Score :0.0 |
| Immunolabeling of distinctive neoepitopes , exposed by cleavage of procaspase 9 at either Asp 315 or Asp 330 , was co localized on these fibrils with active caspase 3 , caspase cleaved cytokeratin 18 , death effector domain containing DNA binding protein and ubiquitin . ^^^ Cytokeratin filaments may thus provide a scaffold whereby active subunits of caspase 9 can activate caspase 3 which , in turn , can activate more caspase 9 so forming an amplification loop to facilitate cleavage of cytokeratin 18 , disruption of the cytoskeleton and the ensuing formation of cytoplasmic inclusions . ^^^ |
|
| Interacting proteins: P05783 and P42574 |
Pubmed |
SVM Score :0.0 |
| Processing of procaspase 3 , caspase 3 enzyme activities , and immunohistochemical staining for cytokeratin 18 cleavage products indicated no activation of caspases . ^^^ |
|
| Interacting proteins: P05783 and P42574 |
Pubmed |
SVM Score :0.0 |
| Addition of MG 132 or lactacystin , 1 h prior to the addition of the CDK inhibitor roscovitine to the cell cultures inhibited apoptosis significantly , as measured by PS exposure , cytokeratin 18 cleavage and caspase 3 activation . ^^^ |
|
| Interacting proteins: P05783 and P42574 |
Pubmed |
SVM Score :0.0 |
| Freshly obtained biopsies were incubated with control medium or medium supplemented with 1 mM DOC for 3 h and then evaluated for apoptotic changes using transmission electron microscopy and immunohistochemical staining for two apoptotic markers , cleaved caspase 3 and cleaved cytokeratin 18 . ^^^ |
|
| Interacting proteins: P05783 and P42574 |
Pubmed |
SVM Score :0.0 |
| Potentially useful apoptosis markers include cleaved cytokeratin 18 ( c CK 18 ) , cleaved caspase 3 ( c cas 3 ) , cleaved lamin A ( c lam A ) , phosphorylated histone H2AX ( gammaH2AX ) , cleaved poly ( ADP ribose ) polymerase ( c PARP ) , and translocation of apoptosis inducing factor ( AIF ) . ^^^ |
|
| Interacting proteins: P05783 and P42574 |
Pubmed |
SVM Score :0.0 |
| Apoptosis was determined by western analysis of the cleavage products of poly ADP ribose polymerase and cytokeratin 18 and caspase 3 activity . ^^^ |
|
| Interacting proteins: P05783 and P42574 |
Pubmed |
SVM Score :0.0 |
| Although the amount of activated caspase 3 did not differ between the two groups of tumors , in primary cell cultures , octreotide was able to increase apoptosis , evaluated by the level of cleaved cytokeratin 18 and the presence of apoptotic nuclei , in GH secreting adenomas , but not in NFPA . ^^^ |
|
| Interacting proteins: P05783 and P42574 |
Pubmed |
SVM Score :0.0 |
| Apoptosis was assessed by detection of cleavage products of poly ADP ribose polymerase , by expression of cleaved cytokeratin 18 intermediate filaments , and by assessment of caspase 3 activity . ^^^ |
|