| Interacting proteins: Q9UMW8 and P05161 |
Pubmed |
SVM Score :0.0 |
| UBP 43 ( USP 18 ) specifically removes ISG 15 from conjugated proteins . ^^^ In our assays , the activity of UBP 43 toward Ub fusions was undetectable in vitro directing us to investigate the possibility of Ub like proteins such as SUMO , Nedd 8 , and ISG 15 as probable substrates . ^^^ We consequently demonstrate that UBP 43 can efficiently cleave only ISG 15 fusions including native ISG 15 conjugates linked via isopeptide bonds . ^^^ Deletion of the UBP 43 gene in mouse leads to a massive increase of ISG 15 conjugates in tissues indicating that UBP 43 is a major ISG 15 specific protease . ^^^ UBP 43 is the first bona fide ISG 15 specific protease reported . ^^^ |
|
| Interacting proteins: Q9UMW8 and P05161 |
Pubmed |
SVM Score :0.0 |
| Lipopolysaccharide activates the expression of ISG 15 specific protease UBP 43 via interferon regulatory factor 3 . ^^^ UBP 43 is a protease that specifically removes a ubiquitin like protein , ISG 15 , from its targets . ^^^ Coordinated induction of ISG 15 and UBP 43 suggests that ISG 15 conjugation is a dynamic process and that a critical balance of ISG 15 modification should be maintained during innate immune response . . ^^^ |
|
| Interacting proteins: Q9UMW8 and P05161 |
Pubmed |
SVM Score :0.0 |
| UBP 43 ( USP 18 ) is a protease that removes the ubiquitin like modifier ISG 15 from conjugated proteins . ^^^ Here we present the first report of dysregulation of protein ISG 15 modification by the generation of UBP 43 knockout mice . ^^^ In the absence of UBP 43 , brain tissue showed an elevated level of ISG 15 conjugates , and cellular necrosis was evident in the ependyma . ^^^ These results demonstrate that UBP 43 plays a critical role in maintaining the homeostatic balance of ISG 15 conjugated protein , and that regulation of cellular levels of ISG 15 protein modification is essential for brain cell function . . ^^^ |
|
| Interacting proteins: Q9UMW8 and P05161 |
Pubmed |
SVM Score :0.0 |
| Like other components of the protein ISGylation system ( ISG 15 , UBE1L , and UBP 43 ) , Ubc 8 is an interferon inducible protein . ^^^ |
|
| Interacting proteins: Q9UMW8 and P05161 |
Pubmed |
SVM Score :0.0 |
| Role of ISG 15 protease UBP 43 ( USP 18 ) in innate immunity to viral infection . ^^^ We have identified UBP 43 ( USP 18 ) as an ISG 15 deconjugating protease . ^^^ This report provides the first direct evidence that the ISG 15 protease UBP 43 and possibly protein ISGylation have a role in innate immunity against viral infection . . ^^^ |
|
| Interacting proteins: Q9UMW8 and P05161 |
Pubmed |
SVM Score :0.0 |
| We have generated a UBP 43 knockout mouse model that is lacking an ISG 15 specific isopeptidase to study the biological role of the protein ISGylation system . ^^^ |
|
| Interacting proteins: Q9UMW8 and P05161 |
Pubmed |
SVM Score :0.0 |
| Mice lacking UBP 43 ( USP 18 ) , the ISG 15 deconjugating enzyme , develop a severe phenotype with brain injuries and lethal hypersensitivity to poly ( 1 : C ) . ^^^ It has been reported that an augmented conjugation of ISG 15 in the absence of UBP 43 induces prolonged STAT 1 phosphorylation and that the ISG 15 conjugation plays an important role in the regulation of JAK / STAT and interferon signaling ( O . ^^^ |
|
| Interacting proteins: Q9UMW8 and P05161 |
Pubmed |
SVM Score :0.0 |
| UBP 43 , an ISG 15 specific deconjugating enzyme : expression , purification , and enzymatic assays . ^^^ UBP 43 activity is not critical for precursor processing , as UBP 43 deficent cells can generate ISG 15 conjugates upon type 1 interferon treatment . ^^^ |
|
| Interacting proteins: Q9UMW8 and P05161 |
Pubmed |
SVM Score :0.0 |
| Here we report that mice lacking UBP 43 , a protease that removes ISG 15 from ISGylated proteins , are hypersensitive to type 1 IFN . ^^^ |
|
| Interacting proteins: Q9UMW8 and P05161 |
Pubmed |
SVM Score :0.0 |
| Loss of UBP 43 ( USP 18 ) , a protease that specifically removes ISG 15 from ISG 15 modified proteins , in mice leads to decreased life span , brain cell injury , and hypersensitivity to interferon stimulation . ^^^ |
|
| Interacting proteins: Q9UMW8 and P05161 |
Pubmed |
SVM Score :0.0 |
| The ISG 15 isopeptidase UBP 43 is regulated by proteolysis via the SCFSkp 2 ubiquitin ligase . ^^^ UBP 43 belongs to the family of ubiquitin isopeptidases and specifically cleaves ISG 15 , a ubiquitin like molecule that is induced by cellular stresses , such as type 1 interferons ( IFN ) , nephrotoxic damage , and bacterial infection . ^^^ The phenotype of UBP 43 knockout mice includes shortened life span , hypersensitivity to IFN , and neuronal damage , suggesting that tight regulation of ISG 15 conjugation is critical for normal cellular function . ^^^ We also show that Skp 2 promotes UBP 43 ubiquitination and degradation , resulting in higher levels of ISG 15 conjugates . ^^^ In Skp 2 / mouse cells , levels of UBP 43 are consistently up regulated , whereas levels of ISG 15 conjugates are reduced . ^^^ |
|
| Interacting proteins: Q9UMW8 and P05161 |
Pubmed |
SVM Score :0.0 |
| UBP 43 is a novel regulator of interferon signaling independent of its ISG 15 isopeptidase activity . ^^^ Lack of Ubp 43 , an IFN inducible ISG 15 deconjugating enzyme , leads to IFN hypersensitivity in ubp 43 / mice , suggesting an important function of Ubp 43 in downregulation of IFN responses . ^^^ Here , we show that Ubp 43 negatively regulates IFN signaling independent of its isopeptidase activity towards ISG 15 . ^^^ |
|
| Interacting proteins: Q9UMW8 and P05161 |
Pubmed |
SVM Score :0.0 |
| The majority of the genes induced in the brain of LCMV cgPi mice were interferon ( IFN ) stimulated genes ( ISGs ) and included the transcription factors STAT 1 and IRF 9 , the ISG 15 protease UBP 43 , and the glucocorticoid attenuated response genes GARG 16 and GARG 49 . ^^^ |
|
| Interacting proteins: Q9UMW8 and P05161 |
Pubmed |
SVM Score :0.0 |
| Reexamination of the role of ubiquitin like modifier ISG 15 in the phenotype of UBP 43 deficient mice . ^^^ The severe phenotype of UBP 43 ( / ) mice characterized by premature death , brain cell injury , and deregulated STAT 1 signaling was ascribed to an enhanced conjugation of ISG 15 . ^^^ To verify the role of ISG 15 in the phenotype of UBP 43 ( / ) mice , we employed mice deficient for both ISG 15 and UBP 43 . ^^^ Here , we show that the phenotype of UBP 43 ( / ) mice was not rescued by the absence of ISG 15 , as evident from unchanged mortality , neurological symptoms , and occurrence of hydrocephalus . ^^^ Presented results clearly demonstrate that the phenotypic alterations of UBP 43 ( / ) mice are not caused by the lack of ISG 15 deconjugation and must be due to another , non ISG 15 mediated molecular mechanism . . ^^^ |
|
| Interacting proteins: Q9UMW8 and P05161 |
Pubmed |
SVM Score :0.0 |
| Like other known components of the protein ISGylation system ( ISG 15 , UBE1L , UBP 43 , and UBC 8 ) , EFP is also an interferon inducible protein . ^^^ |
|
| Interacting proteins: Q9UMW8 and P05161 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UMW8 and P05161 |
Pubmed |
SVM Score :0.0 |
| NA |
|