| Interacting proteins: Q15438 and P05107 |
Pubmed |
SVM Score :0.0 |
| We describe here a protein , cytohesin 1 , which specifically interacts with the intracellular portion of the integrin beta 2 chain ( CD 18 ) . ^^^ |
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| Interacting proteins: Q15438 and P05107 |
Pubmed |
SVM Score :0.0 |
| The PH domain of the closely related protein cytohesin 1 , which , through its Sec 7 homology domain , regulates integrin beta 2 and catalyzes guanine nucleotide exchange of the small guanine nucleotide binding protein ARF 1 , was also found to specifically bind PtdIns ( 3 , 4 , 5 ) P 3 . ^^^ |
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| Interacting proteins: Q15438 and P05107 |
Pubmed |
SVM Score :0.0 |
| In the case of ICAM 1 , apart from ligand itself and the recently cloned molecule cytohesin 1 , the natural physiological regulators of LFA 1 mediated binding to ICAM 1 are unknown . ^^^ |
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| Interacting proteins: Q15438 and P05107 |
Pubmed |
SVM Score :0.0 |
| Monocyte adherence induced by lipopolysaccharide involves CD 14 , LFA 1 , and cytohesin 1 . ^^^ Cytohesin 1 , a phosphatidylinositol 3 , 4 , 5 triphosphate regulated adaptor molecule for LFA 1 activation , was found to be expressed in THP 1wt cells . ^^^ This change in LFA 1 appears to be cytohesin 1 dependent . . ^^^ |
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| Interacting proteins: Q15438 and P05107 |
Pubmed |
SVM Score :0.0 |
| Cytohesin 1 regulates beta 2 integrin mediated adhesion through both ARF GEF function and interaction with LFA 1 . ^^^ Mutational analyses of the beta 2 cytoplasmic domain revealed that the adhesive function of LFA 1 depends on its interaction with cytohesin 1 , unless the integrin is activated by exogenous divalent cations . ^^^ Secondly , cytohesin 1 induces expression of an extracellular activation epitope of LFA 1 , and the exchange factor function is not essential for this activity . ^^^ In contrast , LFA 1 mediated cell adhesion and spreading on intercellular cell adhesion molecule 1 is strongly inhibited by a cytohesin 1 mutant , which fails to catalyze ARF GDP GTP exchange in vitro . ^^^ Thus , cytohesin 1 is involved in the activation of LFA 1 , most probably through direct interaction with the integrin , and induces cell spreading by its ARF GEF activity . ^^^ |
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| Interacting proteins: Q15438 and P05107 |
Pubmed |
SVM Score :0.0 |
| One of them , cytohesin 1 , a 47 kDa cytoplasmic protein acts as an inside out signaling molecule and regulates binding of the beta 2 integrin leukocyte function antigen 1 ( LFA 1 ) to its ligand intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^ |
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| Interacting proteins: Q15438 and P05107 |
Pubmed |
SVM Score :0.0 |
| Cytohesin 1 is a dynamic regulator of distinct LFA 1 functions in leukocyte arrest and transmigration triggered by chemokines . ^^^ Cytohesin 1 is a regulatory interaction partner of the beta 2 integrin alphaLbeta 2 ( LFA 1 ) and a guanine exchange factor ( GEF ) for ADP ribosylation factor ( ARF ) GTPases . ^^^ Overexpression of cytohesin 1 increased LFA 1 dependent arrest of leukocytic cells triggered by chemokines on cytokine activated endothelium in flow while reducing the fraction of rolling cells . ^^^ Expression of these constructs and a beta 2 mutant interrupting the interaction with cytohesin 1 indicated that shape change in flow and transendothelial chemotaxis involve both LFA 1 avidity regulation and GEF activity of cytohesin 1 . ^^^ Our data suggest that cytohesin 1 and ARF 6 are involved in the dynamic regulation of complex signaling pathways and cytoskeletal remodeling processes governing LFA 1 functions in leukocyte recruitment . ^^^ |
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| Interacting proteins: Q15438 and P05107 |
Pubmed |
SVM Score :0.0 |
| Here , intracellular phosphoprotein staining with 13 dimensional flow cytometry showed that LFA 1 activation induced phosphorylation of the beta ( 2 ) integrin chain and release of Jun activating binding protein 1 ( JAB 1 ) , and mediated signaling of kinase Erk1 / 2 through cytohesin 1 . ^^^ |
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| Interacting proteins: Q15438 and P05107 |
Pubmed |
SVM Score :0.0 |
| One of these is an association of the B 2 1 Sec 7 domain with the plasma membrane , binding to the cytoplasmic portion of the integrin beta 2 chain ( CD 18 ) and is postulated to be involved in inside out signaling . ^^^ |
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| Interacting proteins: Q15438 and P05107 |
Pubmed |
SVM Score :0.0 |
| NA |
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