Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.96361113
This is in contrast to neutrophil adhesion to ICAM 1 transfected cells , where LFA 1 binds with a higher avidity than Mac 1 to ICAM 1 . 3 ) Following stimulation , the capacity of LFA 1 to support aggregate formation decreases with time at a rate approximately 3 fold faster than that of Mac 1 . 0.96361113^^^ We report that 1 ) LFA 1 binds ICAM 3 as its primary ligand supporting homotypic adhesion , although the possibility of other ligands was also detected . 2 ) Mac 1 binding to an unidentified ligand supports homotypic adhesion with an efficiency comparable to LFA 1 at low shear rates of approximately 100 s 1 . 0.84119481^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The alphaM subunit of integrin Mac 1 contains several distinct regions in its extracellular segment . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Antibodies to CD11a , CD11b , and CD 18 on PMN also inhibited PMN epithelial adherence . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Intravenous administration of antibody to CD 18 , but not antibody to CD11b , was highly protective against lung injury . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The CD5+ B cells expressed CD11b ( Mac 1 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
CD11a ( LFA 1 ) and CD11b are less expressed , whereas CD11c is preserved . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
CD11b ( Mac 1 ) : a marker for CD8+ cytotoxic T cell activation and memory in virus infection . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The expression of the complement receptors , CR 1 ( CD 35 ) and CR 3 ( CD11b , CD 18 ) , was reduced . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
On the other hand , adhesion of PMNs to fibronectin was inhibited by monoclonal antibodies to CD 18 and to CD11b . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The phosphorylated CD 18 subunit was associated with both CD11a and CD11b . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The expression of CD11a , CD11b , CD11c , CD 15 , CD 18 , CD 10 , CD 24 and B 13 3 membrane antigens was also evaluated . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
CD11b , CD11c , CD 14 and CD 18 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Lipopolysaccharide from P . gingivalis upregulated the CR 3 ( Mac 1 , CD11b ) receptors on PMN . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The human leukocyte adhesion glycoprotein Mac 1 ( complement receptor type 3 , CD11b ) alpha subunit . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Antibodies to CD11a , CD11b , CD11c , CD 15 , CD 17 , CD 18 , and CD 45 do not activate monocytes . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
One of these molecules , made up of CD11b CD 18 ( alpha and beta chains ) , is also the receptor for iC3b . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The majority of them ( 75 95 % ) expressed the CD11b and CD 18 molecules , and 60 70 % expressed CD 54 ( ICAM 1 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Investigation of adhesion molecule expression revealed no differences in CD11b or CD 18 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Using the recently solved structure of the Mac 1 ( CD11b ) 1 domain as a model ( Lee , J . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Flow cytometric analysis revealed higher expression of CD11a , CD11b , CD 18 and CD49d on NK LAK compared with T LAK . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
CD11a , CD11b , CD11c , CD 18 , CD49b , and CD49f failed to demonstrate any immunostaining with type 2 cells . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
It is LFA 1 dependent because CD11a and CD 18 mAbs inhibit homotypic aggregation , whereas CD11b and CD11c mAbs do not . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Mac 1 was determined by an ELISA with monoclonal antibodies that were specific for the alpha chain ( CD11b ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The expression of CD 18 and CD11b on the neutrophil surface was also increased by endothelin 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
CR 3 ( CD11b , CD 18 ) : a phagocyte and NK cell membrane receptor with multiple ligand specificities and functions . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
It was observed that these oxidants can cause up regulation of CD11b and CD 18 expression with shedding of L selectin . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
CD11a , but not CD11b , was responsible for most of the CD 18 mediated binding . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Immunohistochemistry was performed for CD 18 , CD11a , CD11b , CD11c , alpha d , ICAM 1 , ICAM 2 , and ICAM 3 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Adhesion to fibrin alone was inhibited by CD11b and CD 18 blocking antibodies . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies ( mAb ) against CD 18 , CD11b , and ICAM 1 inhibited PMN EC adhesion . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Investigation of cell surface antigen expression revealed no changes in beta 2 integrin ( CD11b / CD 18 ) expression . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Effects of polymeric C 3 , C3b and iC3b on neutrophil expression of CD11b and CD 18 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
This process did not increase production of TNF alpha and did not change the surface expression of CD 18 , CD11a or CD11b . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
CD11b is part of the beta 2 integrin Mac 1 and plays an important role in neutrophil adhesion . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Increased adhesiveness was not associated with an abnormal expression of CD11a , CD11b , CD11c , CD 18 , CD62L , or CD 15 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Leukocyte Mac 1 ( CD11b / CD18 ) and L selectin ( CD62L ) are implicated in leukocyte adhesion to endothelial cells . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Moreover , several integrins ( CD11b , CD11c , CD 18 , CD41a , CD 61 and CD 29 ) were also found . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Immunohistochemistry was performed for CD 18 , CD11a , CD11b , CD11c , alphad , ICAM 1 , ICAM 2 , and ICAM 3 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In particular , the effect of liarozole on the integrin markers CD11b and CD 18 was studied . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The expression of CD11b , CD11c , and CD 18 on human neutrophils after G CSF treatment increased , but CD11a did not . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Monocyte tissue factor ( CD 142 ) and Mac 1 ( CD11b ) are increased in septic patients . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Blocking mAbs against CD 18 and CD11b abrogated fibrinogen induced NF kappa B activation . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
PMNs had increased surface expression of CD11b , CD 18 , CD 14 , CD 32 , and CD 64 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Expression of CD11b ( Mac 1 ) was unchanged . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Like normal blood basophils , they express the integrins , CD11b , CD 18 , CD 29 , and CD49d . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In macrophages PU . 1 regulates a number of genes , including c fms , CD11b , CD 18 , and FcgammaR1b . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The only exception was CD11b ( Mac 1 , CR 3 ) which showed a higher expression in PBM than in AM . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
This up regulation is restricted to specific glycoproteins including CD11b and CD 18 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Mac 1 ( CD11b ) positive leukocytes were localized close to the Schwann cells showing enhanced CD 59 staining . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
BACKGROUND : L selectin ( CD62L ) and Mac 1 ( CD11b / CD18 ) play a crucial role in the infiltration of eosinophils . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
It was not inhibited by antibodies to CD 18 , CD 29 , CD 2 , CD11a and CD11b . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
CD11b , CD 18 ) and exports soluble molecules ( mediators e . g . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
There was no upregulation of Mac 1 ( CD11b / CD 18 ) or shedding of L selectin on circulating neutrophils . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Preincubation of cells with CD11b or CD 18 antibody specifically decreased adhesion to either BSA or ICAM 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
On the other hand , the beta 2 integrins CD11a , CD11b , CD11c and CD 18 were all expressed on MAC and DC . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Finally , H2O2 enhanced the expression of both CD11b and CD 18 on EOS . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The panel of antibodies included CD11b , CD 14 , CD 16 , CD 18 , CD49d , CD62L , CD 64 , CD66b , and HLA DR . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The CD11b , CD 18 , and CD 54 receptors are implicated in CD 34 ( + ) cell adhesion to BM microenvironment . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The CD11b , CD 18 and CD 54 receptors are implicated in CD34+ cells adhesion to BM micro environment . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
CD 18 blockade did not reduce the postischemic rise of CD11b , while fucoidin was inhibitory ( 24 % increase ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
CD11b , CD 18 , CD 31 , and CD62L expressions on circulating PMNs were measured by flow cytometry . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
CD11a , CD11b , and CD 18 expression on lymphocytes and PMN were also measured by flow cytometory . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Fetal granulocytes tend to have a lower expression of CD11b , CD11c , CD 18 , and CD 32 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Furthermore , CD11b and / or CD 18 partially mediated PL induced DC maturation . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
An antibody to CD11b but not CD11a blocked migration to an extent similar as with anti CD 18 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Antibodies against L selectin , but not against CD11a , CD11b , or CD 18 , reduced epsilon at 100 s ( 1 ) by > 85 % . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
CD 18 , CD11a , CD11b , and CD 63 membranal levels were assayed by flow cytometry . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
No differences were detected in CD11b ( Mac 1 ) , CD15s ( sLe ( 10 ) ) , or CD62L ( L selectin ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The expression of CD 18 , CD11b and oxidative burst in granulocytes was measured in whole blood by flow cytometry . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Rabbit MAC 1 receptor , homologue to human CD11b is present in macrophages . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
CD11b and CD 18 although initially unchanged , became down regulated on day 3 ( p < 0 . 05 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Culture of macrophages with PRO 2000 also resulted in diminished detection of the surface proteins CD11b and CD 18 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
LFA 1 , Mac 1 , and p 150 , 95 are leukocyte adhesion proteins called beta 2 integrins . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Circulating integrins : alpha 5 beta 1 , alpha 6 beta 4 and Mac 1 , but not alpha 3 beta 1 , alpha 4 beta 1 or LFA 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The expression of LFA 1 , Mac 1 , and Mac 2 after this treatment was studied by FACS analysis . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
LFA 1 , Mac 1 , and CD 45 ) but expressed ICAM 1 , NCAM , J11d , CD 44 , and MHC molecules . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Other PMN surface markers ( My 4 , My 7 , ICAM 1 , LFA 1 , LFA 3 , Mac 1 ) did not change significantly . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
These anti LFA 1 mAb did not bind to the Mac 1 positive P388D1 cells . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Receptor identity was explored using antibodies to the leukocyte adhesive receptors Mac 1 , LFA 1 , and p150 . 95 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Depletion of CD 4 ( OKT 4 ) , HLADR ( OKIa ) , or LFA 1 ( MAC 1 ) positive populations failed to reduce NK activity . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
LFA 1 , Mac 1 and P150 / 95 ] . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The LFA 1 , Mac 1 leucocyte adhesion glycoprotein family and its deficiency in a heritable human disease . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Compared with LFA 1 and Mac 1 , p 150 , 95 is less well functionally characterized . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Epidermotropic neutrophilic granulocytes express MAC 1 ( CD11b / CD18 ) rather than LFA 1 ( CD11a / CD18 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The beta 2 integrins ( LFA 1 , Mac 1 , and p 150 , 95 ) are critical for many adhesive functions of leukocytes . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Inhibition and stimulation of LFA 1 and Mac 1 functions by antibodies against murine CD 18 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The transition to cell arrest involved both LFA 1 ( CD11a / CD18 ) and Mac 1 ( CD11b / CD18 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In contrast to LFA 1 , Mac 1 appeared to recognize a CD 54 independent ligand constitutively expressed on the hepatocytes . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
ICAM 1 binds to leukocyte function associated antigen ( LFA 1 ) or macrophage 1 antigen ( Mac 1 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Surface bound platelets express ICAM 2 and GPIIbIIIa bound fibrinogen , which are ligands for LFA 1 and MAC 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
LFA 1 and Mac 1 expression or with impairment of polymorphonuclear leukocyte antibacterial activity . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
ICAM 1 binds to leukocyte function associated antigen ( LFA 1 ) or macrophage 1 antigen ( Mac 1 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The enhanced TEM was inhibited by monoclonal antibodies ( mAb ) to LFA 1 , but not by mAb to Mac 1 or to VLA 4 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Cell arrest was dependent on application of shear and activation of Mac 1 and LFA 1 to bind ICAM 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The leukocyte integrins LFA 1 and Mac 1 bind to endothelial intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The loss of Mac 1 was completely prevented by blocking antibodies ( Abs ) directed to FcgammaRII ( CD 32 ) or CD 18 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
All starches regulate the expression of adhesion molecules LFA 1 and ICAM 1 , as well as receptor Mac 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Complement receptor 3 ( CR 3 , Mac 1 , integrin alpha M beta 2 , CD11b / CD18 ) is required for tyrosine phosphorylation of paxillin in adherent and nonadherent neutrophils . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The structural motif glycine 190 valine 202 of the fibrinogen gamma chain interacts with CD11b / CD18 integrin ( alpha M beta 2 , Mac 1 ) and promotes leukocyte adhesion . ^^^ The leukocyte restricted integrin CD11b / CD18 ( alpha M beta 2 , Mac 1 ) is a receptor for fibrinogen on stimulated monocytes and neutrophils . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
This was confirmed using COS 7 and WOP cells doubly transfected with alpha M ( CD11b ) and beta 2 ( CD 18 ) or with alpha L ( CD11a ) and beta 2 when it was found that the Ab bound only the alpha M beta 2 transfectants . ^^^ A common epitope on platelet integrin alpha IIb beta 3 ( glycoprotein IIbIIIa ; CD41b / CD61 ) and alpha M beta 2 ( Mac 1 ; CDIIb / CD18 ) detected by a monoclonal antibody . ^^^ Evidence is presented that the mAb 25E11 , directed against the platelet integrin alpha IIb beta 3 ( glycoprotein IIbIIIa ; CD41b / CD61 ) also binds the distinct myeloid cell integrin alpha M beta 2 ( Mac 1 ; CDIIb / CD18 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
These genes include : Acid phosphatase type 5 ( ACP 5 ) , Cholecystokinin Type B Receptor ( CCKBR ) , Antibiotic Peptide ( FALL 39 ) , Insulin like Growth Factor 1 Receptor ( IGF1R ) , Integrin Alpha M ( ITGAM ) , Integrin Beta 2 ( ITGbeta 2 ) , Opioid Receptor Mu 1 ( OPRM 1 ) , Pro hormone Converter ( PC1 / 3 ) , Retinol Binding Protein 3 ( RBP 3 ) , Ribosomal DNA ( RNR 1 ) , and Zona Pellucida Glycoprotein 1 ( ZP 1 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
This study sets out to investigate how B . burgdorferi binds to human polymorphonuclear leukocytes ( PMNs ) when an exogenous complement is added and how the CR 3 complement receptor , known as Mac 1 or alpha ( m ) beta 2 integrin , is involved in the binding process . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The interaction of leukocyte integrin alphaMbeta 2 ( CD11b / CD18 , Mac 1 ) with fibrinogen has been implicated in the inflammatory response by contributing to leukocyte adhesion to the endothelium and subsequent transmigration . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We have demonstrated in both neutrophils and CHO Mac 1 ( CR 3 integrin ) transfected cells , that one phagocyte receptor which is involved in B . burgdorferi adhesion in non osponic phagocytosis is the CR 3 complement receptor known as integrin alpha m beta 2 . ^^^ Data presented here show that inhibition of adhesion on CR 3 Mac 1 transfected cells and neutrophils is induced by mannose as well as by N acetyl D glucosamine , sugars known to be specific inhibitors of the COOH terminal lectin site of the integrin CR 3 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Amino acid sequences within the alpha subunit of integrin alpha M beta 2 ( Mac 1 ) critical for specific recognition of C3bi . ^^^ This process depends on the interaction between C3bi , a degradation product derived from activation of the complement system , and the alpha M beta 2 ( CD11b / CD18 , Mac 1 ) receptor , the major integrin on neutrophils . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The effect of kininogen adsorption on neutrophil adhesion , the surface density of the adsorbed kininogen , and the exposure of HK domains 3 and 5 ( D ( 3 ) and D ( 5H ) ) , which are responsible for the binding of HK to the neutrophil integrin alpha ( m ) beta ( 2 ) or Mac 1 , were examined . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We deleted the 1 domain from the integrin alpha ( M ) and alpha ( L ) subunits to give 1 less Mac 1 and lymphocyte function associated antigen 1 ( LFA 1 ) , respectively . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Because cellular adhesion is critical for this eosinophil degranulation , we have tested the hypothesis that ligation of the beta ( 2 ) integrin , alpha ( M ) beta ( 2 ) ( Mac 1 , CD11b / CD18 ) , leads to intracellular signaling events that contribute to the eosinophil activation response . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
To elucidate the role of G CSF in controlling hematopoietic cell migration capabilities , we studied inducible expression of the myeloid specific marker , integrin alpha ( M ) beta ( 2 ) ( CD11b / CD18 , Mac 1 ) , in the myeloid cell line , 32D . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Fibrinogen is a ligand for leukocyte integrin alpha ( M ) beta 2 ( CD11b / CD18 , Mac 1 ) and mediates adhesion and migration of leukocytes during the immune inflammatory responses . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Previous studies demonstrated that integrin alpha ( M ) beta ( 2 ) ( CD11b / 18 , Mac 1 ) forms a physical complex with the urokinase type plasminogen activator receptor ( uPAR / CD87 ) on leukocytes . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The integrin alpha ( M ) beta ( 2 ) ( CD11b / CD18 , Mac 1 ) is progressively upregulated to high levels on migrating phagocytic leukocytes in response to inflammatory stimuli and is able to bind numerous ligands in the interstitial matrix . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The interaction between the leukocyte integrin alpha ( M ) beta ( 2 ) ( CD11b / CD18 , Mac 1 , CR 3 ) and fibrinogen mediates the recruitment of phagocytes during the inflammatory response . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
These activities are enhanced by binding of growth factors like fibroblast growth factor 2 ( FGF 2 ) and vascular endothelial growth factor ( VEGF ) , and cytokines like interleukin ( IL ) 1 . ( 6 ) Fibrinogen binding to the platelet alpha ( IIb ) beta 3 receptor , which is important for incorporating platelets into a developing thrombus . ( 7 ) Leukocyte binding to fibrin ( ogen ) via integrin alpha ( M ) beta 2 ( Mac 1 ) , which is a high affinity receptor on stimulated monocytes and neutrophils . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The process is triggered by the interaction of ligands on the pathogens ' surface with specific phagocytic receptors , including immunoglobulin ( FcR ) and complement C3bi ( CR 3 ) receptors ( integrin alpha ( M ) beta 2 , Mac 1 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Furthermore , results indicated that one of the genes that did not change among individuals but specifically increased after CHI and decreased after NAP treatment was the cell surface glycoprotein Mac 1 ( CD11B antigen ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Furthermore , closed head injury was associated with a delayed increase in the expression of the immune cell surface glycoprotein Mac 1 ( CD11B antigen ) at the injury site that was decreased in NAP treated mice . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Serum free culture of human monocytes in the presence of monoclonal antibodies to the LFA 1 alpha chain ( CD11a ) , CR 3 alpha chain ( CD11b ) or beta chain ( CD 18 ) bound to Sepharose induced the dose dependent production of cell associated interleukin ( IL ) 1 activity and of IL 1 alpha and IL 1 beta antigens , but no release of extracellular IL 1 activity or antigen in the culture medium . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Regulation of expression of the CD11b and CD 18 subunits of the neutrophil adherence receptor during human myeloid differentiation . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The most pronounced changes were observed in the surface receptors on CD 4 ( `` helper cells ' ' ) cells and leukocytes bearing the CD11b ( Mac 1 ) and other integrin and adhesion receptors . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies ( MoAbs ) against CD11a , CD11b , CD 18 , and CD 54 and a polyclonal anti human fibronectin antibody do not inhibit attachment of either B or T lymphoblastic cells to MSC . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
This protective effect of IFN gamma on PMN function was associated with the prolonged surface expression of the complement receptor three ( CR 3 ) alpha chain ( CD11b ) , CR 3 beta chain ( CD 18 ) , FcRII ( CD 32 ) , and FcRIII ( CD 16 ) , and the appearance of surface FcRI ( CD 64 ) . ^^^ Using this procedure , mRNA for gp 91 phox , p 67 phox , p 47 phox , CD 64 , two forms of CD 32 , CD 16 , CD11b , CD 18 , and actin were found to be depressed after overnight storage of neutrophils , and this decrease in steady state mRNA levels was in part or totally prevented by IFN gamma . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Simultaneous mobilization of Mac 1 ( CD11b / CD18 ) and formyl peptide chemoattractant receptors in human neutrophils . ^^^ Mobilization of a distinct subset of specific granules provides a physiologically important mechanism to recruit Mac 1 ( CD11b / CD18 ) from an intracellular pool to the external surface of the neutrophil plasma membrane , where the functionally active heterodimer mediates several adherence dependent processes that are crucial for adequate host defense and cellular inflammatory responses . ^^^ Under a variety of conditions , FPR levels correlated with Mac 1 ( CD11b ) expression on cell populations selected on the basis of CD11b fluorescence intensity . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Inhibition of intravascular leukocyte leukocyte aggregation with mAb against CD11b ( Mac 1 ) showed partial inhibition of hemorrhage , while mAb against CD11a ( LFA 1 ) showed no inhibitory activity . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
To determine the functional importance of these changes , cDNA encoding a normal alpha chain ( CD11b ) was cotransfected into COS with CD 18 cDNAs encoding for wild type , maternal mutant allele , or CD 18 containing N 351 S substitution . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The roles of beta 2 integrin molecules in neutrophil accumulation and tissue injury have been examined by the use of antibodies that are reactive with human CD11b and CD 18 and cross react with the homologous epitopes on rat neutrophils . ^^^ Adherence to rat pulmonary artery endothelial cells by human neutrophils and endothelial cell killing by phorbol ester activated human neutrophils required CD11b , CD11c , and CD 18 . ^^^ Companion adherence studies between rat neutrophils and endothelial cells revealed a requirement for both CD11b and CD 18 . ^^^ Neither anti CD11b nor anti CD 18 depressed in vitro responses ( O 2 generation and chemotactic migration ) of rat neutrophils . ^^^ The accumulation of neutrophils in glycogen induced peritoneal exudates was diminished substantially in rats treated with either anti CD 18 or anti CD11b . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The leukocyte integrins consist of a common CD 18 ( beta ) subunit and individual , noncovalently associated alpha subunits designated CD11a , CD11b , and CD11c . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
MoAb to CD11b , CD11c , and CD 18 each efficiently blocked ADCC by normal PMN . ^^^ Granulocyte macrophage colony stimulating factor ( GM CSF ) significantly increased the expression of CD11b , CD11c , and CD 18 on normal PMN , decreased the expression of Fc receptors ( FcR ) , and enhanced ADCC by normal but not by LAD PMN . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The CD11b ( or macrophage 1 antigen ; MAC 1 ) subunit of the leukocyte integrin family forms a noncovalently associated heterodimeric structure with the CD 18 ( beta ) subunit on the surface of human granulocytes and monocyte / macrophages , where it enables these myeloid cells to participate in a variety of adherence related activities . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Positivity of CD 18 Ad cells with the common beta 2 integrin ( CD 18 ) is largely attributable to expression of alpha L ( CD11a ) chain , rather than alpha M ( CD11b ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We found that MCP 1 induced expression of CD11c ( p 150 , 95 alpha subunit ) and CD11b ( Mac 1 alpha subunit ) , and caused little or no change of CD11a ( lymphocyte function associated Ag 1 alpha subunit ) , very late activation Ag 4 , or leukocyte adhesion molecule 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The adherence of both L 1210 and L5178Y to MSC was unaffected by rat monoclonal antibodies to murine CD11a , CD11b , and CD 18 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Flow cytometric analysis of adhesion molecules expressed on neutrophils revealed that pretreatment of neutrophils with NPC 15669 prior to activation inhibits the increase in expression of the CD11b and CD 18 adhesion molecule subunits . ^^^ We conclude that ( 1 ) NPC 15669 acts on neutrophils to block activation induced adherence , and ( 2 ) NPC 15669 inhibits the upregulation of the CD11b / CD18 ( Mac 1 ) adhesion receptor . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
This A / R induced hyperadherence was significantly diminished by monoclonal antibodies ( MAb ) directed at CD11a , CD11b , CD 18 or ICAM 1 , but not by MAb directed at ELAM 1 . ^^^ The inhibitory effects of anti CD11a and anti CD11b were additive and equivalent to that of anti CD 18 MAb . ^^^ However , immunofluorescence flow cytometry indicated that incubation of neutrophils in supernatants of A / R conditioned HUVEC elicited an increase of surface CD11b and CD 18 , but not CD11a . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Investigation of 12 Irish setter puppies from six litters with severe recurrent infections , neutrophilia and low body weight revealed a leucocyte adhesion protein deficiency with a total lack of CD11b and CD 18 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Ling Zhi 8 ( LZ 8 ) , a novel immunomodulatory protein , markedly enhanced the expression of CD11b , but not CD11a , CD 13 , CD 14 , CD 18 , CD 33 or HLA DR , on the U 937 cell line in a dose dependent fashion . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The expression of three alpha chains designed as CD11a , CD11b , CD11c , a common beta chain CD 18 , and of a ligand for several integrins CD 54 ( ICAM 1 ) was studied on alveolar macrophages of patients with active and inactive sarcoidosis and in control subjects . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In vitro , monoclonal antibodies ( MoAbs ) to CD 18 and CD11b were able to inhibit neutrophil aggregation and adhesion to endothelium . ^^^ FMLP induced neutrophil aggregation was inhibited by 39 . 8 % + / 11 . 5 % and 44 . 8 % + / 12 . 3 % , respectively , by MoAbs to CD 18 and CD11b ( P less than . 0005 , n = 4 for both ) ; a similar effect was demonstrated on TPA induced aggregation . ^^^ MoAb CD 18 reduced the adhesion of unstimulated neutrophils to endothelium by 44 % ( P less than . 01 , n = 7 ) , and inhibited the amount of GM CSF stimulated adhesion by 74 % ( P less than . 001 , n = 7 ) , while MoAb to CD11b produced a reduction of unstimulated neutrophil adhesion by 30 % , and of GM CSF stimulated adhesion by 40 % ( P less than . 01 , n = 5 , for both ) . ^^^ However , when administered in vivo , MoAb CD 18 produced only a small , albeit significant , amelioration of GM CSF induced margination in vivo , while MoAb CD11b was without effect . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The mean fluorescence intensity ( MFI ) of monocyte CD11a was enhanced by interleukin 2 ( IL 2 ) , TNF alpha and TNF beta , and that of CD11b , CD11c and CD 18 was increased by IL 2 , IL 4 , TNF alpha and TNF beta . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In vitro investigations with each of the four methylxanthines showed two of these metabolites to be most effective at reducing PMN respiratory burst activity , lactoferrin release , and the expression of CD11b and CD 18 molecules . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Fluorescence and neutrophil / myocyte adherence followed the same time course , and both were blocked by monoclonal antibodies to CD 18 , CD11b , and ICAM 1 , but mAb R7 . 1 , recognizing a functional epitope on CD11a , was not inhibitory . ^^^ Thus , adherence dependent on Mac 1 ( CD11b / CD18 ) and ICAM 1 ( CD 54 ) activates the neutrophil respiratory burst resulting in a highly compartmented iron dependent myocyte oxidative injury . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The beta 2 ( B 2 ) integrin ( leukocyte integrin , CD11 / CD18 ) subfamily comprising three members , each consisting of a shared beta subunit ( CD 18 ) non covalently associated with unique alpha subunits ( CD11a , CD11b , CD11c ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Treatment of the PMN with mAb against CD11a , CD11b , or CD 18 markedly reduced PMN adhesion to PiV 2 infected 1 degree TEC and 9HTEo cells . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
These results suggest that occupancy of CD 18 transduces a stimulatory Ca2+ signal that is critically regulated by the state of cell activation / differentiation and by the association with the unique alpha subunit CD11b . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
These nonphagocytic cells appear to be related to follicular dendritic cell ( FDC ) as they do not have properties of monocytic lineage cells or dendritic cells and because , like FDC , 1 ) they express CD11b , CD 14 , CD 29 , CD 40 , CD 54 , CD 73 , CD 74 , and VCAM 1 , and do not express CD11c , CD 22 , T cell markers , CD 18 , CD 25 and CD 45 ; and 2 ) they bind human B lymphocytes and B cell lines , but not T lymphocytes by an adhesion blocked in part by mAb to VLA 4 ( CD49d ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Blockade of CD11a , CD11b and CD 18 with saturating concentrations of specific mAb did not inhibit the release of cytokines from LPS stimulated monocytes . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Combinations of G CSF and RA produced a supra additive increase in the percentage of WEHI 3B D+ cells reducing nitro blue tetrazolium and expressing Mac 1 ( CD11b ) antigen on the cell surface , two markers of the mature state . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Other molecules involved in cell / cell adhesion are LFA 1 ( CD11a / CD18 ) , Mac 1 ( CD11b / CD18 ) and their ligand ICAM 1 ( CD 54 ) , p 150 , 95 ( CD11c / CD18 ) , LFA 3 ( CD 58 ) , CD 44 , and LECAM 1 . ^^^ Thymic B cell lymphomas consistently lacked VLA 1 , 2 , 3 , 5 , 6 , and CD11b , expressed ICAM 1 in 21 of 23 cases but were heterogenous for VLA 4 , LFA 1 , CD11c , LFA 3 , CD 44 , and LECAM 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The HC were shown to express the integrin beta 1 subfamily molecules , LFA 1 ( CD11a ) and p 150 , 95 ( CD11c ) in high density but lacked CR 3 ( CD11b ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Differentiation of promyelocytic HL 60 cells along the pathway toward granulocytes using dimethyl sulfoxide ( DMSO ) led to increased expression of the adhesion molecules CD11b ( Mac 1 ) , CD11c ( p 150 , 95 ) , and CD 35 ( CR 1 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
By analytic flow cytometry , the mice immunized with PRP OMPC demonstrated an increase in large splenocytes expressing the Ag Mac 1 ( CD11b , CR 3 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The mean ( SE ) percentages of lymphocytes expressing CD11a ( RA 93 . 4 ( 1 . 7 ) % ; controls 97 . 2 ( 1 . 8 ) % ) and CD 18 ( RA 91 . 3 ( 2 . 3 ) % ; controls 97 . 0 ( 2 . 6 ) % ) were lower and the percentage of monocytes expressing CD11b ( RA 86 . 9 ( 11 . 4 ) % ; controls 78 . 4 ( 11 . 9 ) % ) and CR 1 ( RA 62 . 6 ( 15 . 5 ) % ; controls 36 . 6 ( 17 . 6 ) % ) were higher in patients with RA than in controls . ^^^ In addition , the mean fluorescence intensity of CD 18 ( RA 22 . 1 ( 2 . 3 ) ; controls 30 . 7 ( 2 . 5 ) ) on lymphocytes was decreased and that of CD11b ( RA 4 . 5 ( 0 . 8 ) ; controls 2 . 9 ( 0 . 9 ) ) and CR 1 ( RA 2 . 4 ( 0 . 4 ) ; controls 1 . 5 ( 0 . 5 ) ) on monocytes was increased in patients with RA compared with controls . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
A monoclonal antibody ( 1B4 ) directed toward the beta 2 chain of the integrin receptor for fibrinogen ( CD11b , CD 18 ) completely inhibited the rTNF alpha induced adhesion . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The cDNA for the common beta Mac 1 subunit ( CD11b ) of the mouse LFA 1 / Mac 1 / p150 , 95 group of leukocyte cell adhesion receptors , formally designated integrin beta 2 , has been cloned and sequenced . ^^^ Mouse macrophage beta subunit ( CD11b ) cDNA for the CR 3 complement receptor / Mac 1 antigen . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies R15 . 7 ( anti CD 18 ) , R7 . 1 ( anti CD11a , LFA 1 ) , and R6 . 5 ( anti ICAM 1 ) , administered intravenously before C5a exposure , strongly inhibited leukocyte adherence while antibody LM 2 ( anti CD11b , Mac 1 ) produced significant , but weaker , inhibition . ^^^ These data confirm earlier findings establishing a role for CD 18 in leukocyte adhesion in vivo and extend those observations to implicate both CD11a and CD11b in that adhesion . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The role of leucocyte integrins in phagocyte function has been studied by comparing normal neutrophils with those from a patient with partial leucocyte adhesion deficiency ( LAD ) , in whom the levels of CD11b and CD11c were 10 % of controls , whereas CD11a levels were normal . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
GM CSF increased the expression of the glycoproteins CD11B and CD 18 on the surface of his neutrophils , which may have increased the adhesiveness of neutrophils to the pulmonary endothelium . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
This study investigated the cell surface expression of CD11b ( Mo 1 , Mac 1 , CR 3 ) by neutrophils ( PMNs ) from human crevicular fluid ( CF PMNs ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
These markers include the arrest of cell proliferation , production of reactive oxygen species , changes in the cell surface expression of differentiation associated antigens such as the transferrin receptor , CD11b and CD11c and changes in the levels of several specific gene transcripts such as CD 18 antigen , c myc , ornithine decarboxylase and vimentin . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The expression of the adhesion molecules ( CD11a , b , c / CD18 ) of peritoneal neutrophils , which was determined by an immunofluorescence study , was about four times as much in CD11b and twice as much in CD11c and CD 18 compared to that in blood neutrophils . ^^^ The enhanced adherence activity of peritoneal neutrophils to tumor cells was significantly inhibited by pretreatment of the neutrophils with anti CD11b and anti CD 18 monoclonal antibodies ( mAb ) , but not by pretreatment with anti CD11a or anti CD11c mAb . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Components involved in superoxide anion production ( cytochrome b ) and in cell adhesion processes ( CD11b , CD11c , CD 18 ) , two early functional responses of neutrophils during acute inflammation , are intracellularly located in resting human neutrophils . ^^^ We have found a correlation between secretion of gelatinase and overexpression in the plasma membrane of CD11b , CD11c , CD 18 and cytochrome b upon cell activation . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Exposure of human neutrophils in suspension to CD11b or CD 18 monoclonal antibodies ( mAbs ) 2 does not affect the resting level of cytosolic free Ca2+ in these cells ; however , a subsequent cross linking of either of these antibodies triggers a prompt and significant cytosolic free Ca2+ transient lasting about 10 min . ^^^ The rise in cytosolic free Ca2+ ( from 130 + / 2 to 414 + / 12 nM or 111 + / 12 to 331 + / 22 nM caused by cross linking of CD11b or CD 18 subunits , respectively ) is due to both mobilization of Ca2+ from intracellular stores and influx of Ca2+ across the plasma membrane . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
When the role of different a chains was studied , anti alpha L ( CD11a , LFA 1 ) , anti alpha M ( CD11b , Mac 1 ) and anti alpha 10 ( CD11c , p 150 , 95 ) caused marginal inhibition , but the effect of the 3 combined was comparable to that of anti CD 18 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies ( MoAbs ) to CD11a , CD11b , and CD 18 on the neutrophils inhibited this migration by 52 % + / 11 % , 29 % + / 19 % , and 90 % + / 7 % , respectively . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies to CD11a , CD11b , or CD 18 inhibited the binding of the bacteria to intact human granulocytes by 55 to 80 % , whereas antibodies against other leukocyte surface antigens were not inhibitory . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
By modifying our microassay , we were able to assess PMN transmigration across T 84 monolayers in the physiological direction ( which , for technical reasons , has not been studied in epithelia ) : transmigration was again largely attenuated by MAb to CD 18 or CD11b ( 86 + / 2 % and 73 + / 3 % inhibition , respectively ) but was unaffected by MAb to CD11a , CD11c . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Increases of CD 10 , CD 13 , and CD11b but not CD11a ( LFA 1 ) were also observed in leukocytes from patients undergoing hemodialysis with cuprophan membranes . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The three molecules consist of a specific alpha chain ( CD11a , CD11b , or CD11c ) and share a common beta chain ( CD 18 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Whereas expression of CD11b and CD11c is almost exclusively restricted to cells of the myeloid lineage , that of CD11a and CD 18 is panleukocytic . ^^^ Further analysis of this region of the CD11b gene and comparison with the promoters of the CD11a , CD11c , and CD 18 genes should lead to significant insights into the molecular mechanisms by which these genes are regulated during hematopoietic development and upon activation . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Treatment of U 937 cells with PMA markedly increased CD 14 , CD11a , CD11b and CD 18 antigen expression , and slightly increased CD11c expression . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
These glycoproteins include the complement receptor and adhesion molecule , CR 3 , in which the beta chain ( CD 18 , 105 kDa ) shows much greater expression than the alpha chain ( CD11b , 165 kDa ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Monocyte adhesion induced by anti CD 14 mAb required neither Fcy gamma R engagement nor cross linking of CD 14 , because adhesion was induced by F ( ab ) ' 2 fragments , as well as by monovalent F ( ab ) fragments of anti CD 14 mAb . mAb to CD11a , CD 18 , and intercellular adhesion molecule 1 ( ICAM 1 ) , but not antibodies to CD11b and CD11c , inhibited monocyte adhesion induced by CD 14 engagement . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Under experimental conditions that permitted histamine release , no correlation was seen between the ability of rIL 3 ( 0 . 3 to 300 ng / ml ) to induce histamine release or enhance adhesiveness ( n = 8 ) . mAb blocking experiments demonstrated a role for both CD 11 and CD 18 adherence glycoproteins in basophil adherence induced by rIL 3 , and indirect immunofluorescence and flow cytometric analysis revealed that rIL 3 treatment led to rapid and sustained increases in cell surface expression of CD11b antigens on basophils but not neutrophils ( e . g . , after 10 min : 217 + / 29 vs 91 + / 11 % of control mean fluorescence intensity , p less than 0 . 05 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The overall molecular organization of this family of leukocyte integrins was similar to the human counterpart , and consisted of a common beta subunit of 95 kD ( CD 18 ) and non covalently linked alpha subunits of 150 kD ( CD11C ) , 165 kD ( CD11b ) and 180 kD ( Cd11a ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Using specific mAb , we showed in this in vitro study that the CD 18 beta 2 chain and the CD11b alpha M chain of the CD11 / CD18 integrin heterodimer have a major role in both TNF alpha induced neutrophil mediated detachment of human umbilical vein endothelial cells and H2O2 release by TNF alpha activated human neutrophils . ^^^ In contrast to anti CD 18 mAb , which consistently prevented neutrophil activation , anti CD11a mAb and two of three anti CD11b mAb did not reduce endothelial cell detachment and neutrophil H2O2 release , although they decreased neutrophil adhesion to human umbilical vein endothelial cells . mAb 904 , directed against the bacterial LPS binding region of CD11b , reduced endothelial cell detachment for about 40 % and neutrophil H2O2 release for more than 50 % , demonstrating that CD11b / CD18 is engaged in TNF induced neutrophil activation . ^^^ Dependence on CD11b / CD18 could not be overcome by CD 18 independent anchoring of neutrophils via PHA . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Adhesion was markedly inhibited by MAb against CD 18 and CD11b , with lesser or no inhibition being afforded by MAb against CD11a , CD11c , or ICAM 1 . ^^^ These actions were blocked by actinomycin D or cycloheximide and by MAb against HMC ICAM 1 or phagocyte CD 18 , CD11a , or CD11b , suggesting that cytokines provoked adhesion by inducing HMC ICAM 1 synthesis . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In particular , QE7 . 3E8 stains lymphoid tissue with a distribution clearly distinguishable from CD 18 antibodies ; it precipitates four bands from a T cell line which does not express the CD11b and CD11c alpha chains , and cross immunoprecipitation with QE7 . 3E8 and a CD11a antibody show that the molecules recognized by these antibodies are not associated . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Pretreatment of animals with monoclonal antibodies against the CD11b and CD 18 subunits of the CD11 / CD18 integrin family on the surface of the PMN reduced the number of PMNs in the liver by 50 % and significantly attenuated liver injury and GSSG formation . ^^^ It is concluded that PMNs are actively involved in the pathogenesis of galactosamine and endotoxin shock and that at least in part the accumulation of PMNs , the subsequent oxidant stress , and the tissue injury in this model of experimental hepatitis are CD11b / CD18 ( Mac 1 ) dependent . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Preincubating AM with a monoclonal antibody to CD 18 reduced the adhesion of AM to MDCK cells by 40 % while a significant reduction could not be demonstrated after preincubation with antibodies to either CD11b or CD11c . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Adhesion was dependent on the leucocyte integrin family of glycoproteins , notably on Mac 1 , since monoclonal antibodies toward the beta chain ( CD 18 ) and alpha chain ( CD11b ) of Mac 1 almost completely suppressed PMA induced PMN adhesion ( 88 % and 77 % inhibition , respectively ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
To determine if the movement alone or adhesion alone is responsible for [ Ca2+ ] 1 activity , we treated cells with antibodies against the beta chain ( CD 18 , beta 2 ) or the alpha subunit ( CD11b , alpha m ) of the dominant leukocyte integrin ( CR 3 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Surface antigens CD11b , CD11c , CD 2 , and CD 18 on nonadherent NK cells were clustered at the cellular poles by both immunofluorescence and immunogold electron microscopy , whereas CD11a ( LFA 1 ) and CD 56 antigens were distributed diffusely . ^^^ An antibody ( LeuM 5 ) to the CD11c antigen ( p150 . 95 ) gave the highest level of inhibition , and anti CD11a ( LFA 1 ) also was inhibitory , while anti CD 56 ( NKH 1 ) or anti CD11b did not interfere with adhesion to plastic . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We have shown that CD 14 , CR 1 ( CD 35 ) and CR 3 ( CD11b , CD 18 ) are expressed on the majority of monocytes from all subjects , but on only a minority of normal AM . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The PMNL migration was highly dependent on the function of both CD11a ( LFA 1 ) and CD11b ( MAC 1 ) PMNL adhesion molecules , since monoclonal antibodies to either inhibited migration by about 80 % . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
However , chemotaxis in response to zymosan activated serum ( ZAS ) and f Met Leu Phe ( f MLP ) , was further impaired on the last day of treatment , which was associated with a marked increase in the expression of the granulocyte adhesion receptors CD11a ( P = 0 . 01 ) , CD11b ( P = 0 . 002 ) , CD11c ( P = 0 . 00015 ) and CD 18 ( P = 0 . 0014 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The phosphorylated species on neutrophils were readily detected by immunoprecipitation with each CD 18 mAb and most but not all CD11b mAb which otherwise precipitated 125I labeled CD11b species suggesting that the CD11b alpha chain labelled with 32P may differ slightly from the 125I labeled species in terms of its recognition by certain CD11b mAb . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We have characterized the binding and phagocytosis of Hc yeasts by human PMN and quantified the PMN respiratory burst in response to this organism . mAb specific for CD11a , CD11b , and CD11c all partially blocked the attachment of unopsonized yeasts to PMN ; a mAb to CD 18 inhibited attachment by greater than 90 % . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Both phases of stimulated adhesion were partially inhibited by a monoclonal antibody to CD 18 , the common beta chain of the leukocyte functional Ag family of adhesion molecules , but not by an antibody to CD11b , the alpha chain of MAC 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In normal monocytes , CD 18 and CD11c are concentrated at specific adhesion sites , named podosomes , together with actin , vinculin , and talin , while CD11a , CD11b , CD29 / beta 1 , CDw49d / alpha 4 and CD54 / ICAM 1 retain a diffuse distribution on the cell surface without a selective pattern of localization . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Resting microglia were found to express constitutively CD11a ( LFA 1 ) , CD11b ( Mac 1 , CR 3 ) , CD11c ( P 150 , 95 ; CR 4 ) , and CD 18 ( beta 2 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
GM CSF treated PMN synthesized 4 . 5 fold more class 1 protein , 3 . 7 fold more CR 1 , 2 . 4 fold more CD11b and 3 . 4 fold more CR 3 beta chain ( CD 18 ) , compared with untreated control cells . ^^^ Polymorphonuclear leukocytes ( PMN ) constitutively synthesize various plasma membrane proteins including CR 1 ( 3 ) ( CD 35 ) , CR 3 ( or Mac 1 ) alpha chain ( CD11b ) and MHC class 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Incubation of human bloodstream neutrophils with 50 u / ml recombinant granulocyte macrophage colony stimulating factor ( rGM CSF ) `` primed ' ' the respiratory burst ( as assessed by fMet Leu Phe stimulated luminol dependent chemiluminescence ) and resulted in a rapid ( within 15 min ) up regulation of expression of CD11b and CD 18 ( as measured by FACS analysis ) . ^^^ When neutrophils were incubated for up to 5 h in culture , the fluorescence distributions of CD11b and CD 18 declined indicating the loss of expression of these receptors as the neutrophils aged , but in rGM CSF treated suspensions receptor expression was maintained . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Antibodies to either CD11a , CD11b or CD11c did not inhibit adhesion when used separately , whereas as a mixture their inhibitory capacity was as strong as that of anti CD 18 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Indeed , preincubation with antibodies to either CD 18 or CD11b , but not to CD11c , inhibited the oscillations of cytosolic free Ca2+ in adherent neutrophils . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In order to evaluate the functions of lymphocyte function antigen 1 ( LFA 1 ) ( CD11a / CD18 ) and Mac 1 ( CD11b / CD18 ) on neonatal neutrophils , we examined neutrophil adhesion to and migration through human umbilical vein endothelial cell ( HUVEC ) monolayers in vitro . ^^^ This migration was significantly inhibited by monoclonal antibodies ( MoAbs ) against LFA 1 ( CD11a ) and Mac 1 ( CD11b ) subunits . ^^^ Impaired transendothelial migration by neonatal neutrophils : abnormalities of Mac 1 ( CD11b / CD18 ) dependent adherence reactions . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In contrast , mAb against the alpha subunits of Mac 1 ( CD11b ) and p150 . 95 ( CD11c ) were hardly effective . fMLP mediated neutrophil adhesion was reduced to below baseline levels by anti LeuCAM ( CD 18 ) mAb , whereas the LeuCAM contribution in IL 1 mediated neutrophil adhesion was less pronounced and varied in time . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The leukocyte cell adhesion receptors , complexes of the cluster of differentiation antigen 11a with cluster of differentiation antigen 18 ( CD11a / CD18 ) , cluster of differentiation antigen 11b with cluster of differentiation antigen 18 ( CD11b CD 18 ) and cluster of differentiation antigen 11c with cluster of differentiation antigen 18 ( CD11c CD 18 ) , are of major importance in several leukocyte functions . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies recognizing CD 18 , CD11a , CD11b , and neutrophil lectin adhesion molecule 1 ( LECAM 1 ) , i . e . , the human homologue of the murine MEL 14 antigen , were used to assess the relative contribution of these glycoproteins to neutrophil endothelial adhesion . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Uninvolved keratinocytes were found to stain with anti CD 58 ( leucocyte function associated antigen 3 , LFA 3 ) and there was weak expression of CD11b ( alpha chain of complement C3bi receptor ) and CD11c ( alpha chain of p 150 , 95 molecule ) but not CD11a ( leucocyte function associated antigen 1 , LFA 1 , alpha chain ) on those cells . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Synovial lining cells express numerous macrophage associated antigens , including CD11b ( CR 3 ) , CD 13 , CD 14 , CD 16 ( FcRIII ) , CD 18 , CD 32 ( FcRII ) , CD 45 ( leucocyte common antigen ) , CD 54 ( ICAM 1 ) , CD 64 ( FcRI ) , CD 68 , and CD 71 ( transferrin receptor ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Immunocytochemical investigations using anti CD11a , CD11b and CD 18 monoclonal antibodies on fresh tissue or , better , peripheral leucocytes are necessary to confirm the diagnosis of this uncommon immunological autosomic recessive inherited disorder . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Among the cytotoxic cells ( CD 8 ) and the immunoregulatory subpopulations associated with an immunosuppressive response ( CD8+ CD11b+ , CD8+ CD11b and CD8+ leu8+ ) or with a contrasuppressive response ( Vicia villosa cells ) , an heterogeneous LFA 1 expression was observed , discriminating the LFA 1 dim and the LFA 1 bright cells . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
FMLP stimulated neutrophils from patients with CD 18 deficiency failed to adhere to any of these surfaces and failed to produce H2O2 under these conditions . mAb reactive with CD 18 and CD11b were equally effective in markedly inhibiting the adhesion of normal human neutrophils to these surfaces and markedly inhibited the production of H2O2 . ^^^ A mAb reactive with CD 18 blocked adhesion of stimulated canine neutrophils , and mAb directed against both CD 18 and CD11b blocked H2O2 production by canine neutrophils on KLH and endothelium . ^^^ Mac 1 ( CD11b / CD18 ) mediates adherence dependent hydrogen peroxide production by human and canine neutrophils . ^^^ To characterize the mechanisms involved in this adherence dependent respiratory burst , we have investigated the possible role of one integrin known to participate in the adhesion of neutrophils to endothelial cells , CD11b / CD18 ( Mac 1 ) . ^^^ These results indicate that Mac 1 ( CD11b / CD18 ) can mediate adhesion dependent H2O2 production by human and canine neutrophils exposed to chemotactic factors . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
One , 2E6 , bound a determinant common to all members of the CD11 / CD18 family , most likely the shared 90 kD CD 18 beta chain . 2E6 immunoprecipitated the characteristic beta 2 integrin heterodimers from lymphocytes ( p 180 , 90 ; CD11a ) and macrophages ( p 170 , 90 ; CD11b ) , but from dendritic cells , a p 150 , 90 ( presumably CD11c ) integrin was the predominant species . 2E6 inhibited the binding function of the CD11a and CD11b integrins on B cells and macrophages in appropriate assays , but 2E6 exerted little or no inhibition on the clustering of dendritic cells to T cells early in primary MLR , suggesting a CD11 / CD18 independent mechanism for this binding . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Mo 1 ( complement receptor type 3 , CR 3 ; CD11b / CD18 ) is an adhesion promoting human leukocyte surface membrane heterodimer ( alpha subunit 155 kD [ CD11b ] noncovalently linked to a beta subunit of 95 kD [ CD 18 ] ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The leucocyte surface glycoproteins CD11a ( gp 160 , LFA 1 antigen , TA 1 antigen ) , CD11b ( gp 155 , Mac 1 antigen , OKM 1 antigen , Mo 1 antigen ) , CD11c ( gp 130 , Leu M 5 antigen ) , and CD 18 ( gp 90 ) constitute three heterodimers with different alpha chains and a common beta chain . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The CD11 / CD18 family of leukocyte adhesion promoting proteins is comprised of three members , each composed of a shared beta subunit ( CD 18 ) noncovalently associated with unique alpha subunits ( CD11a , CD11b and CD11c respectively ) . ^^^ A vast series of immunostaining procedures was carried out , using monoclonal antibodies anti CD11a , CD11b , CD11c , and CD 18 . ^^^ The results demonstrated the expression on the membrane of Langerhans cells of the CD11b , CD11c and CD 18 antigens , thus indicating that at least both the H Mac 1 ( CD11b / CD18 ) and the gp 150 , 95 ( CD11c / CD18 ) members of the CD11 / CD18 family are detectable on the cell surface of human resting Langerhans cells . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
These antibodies immunoprecipitated pig proteins corresponding to the human alpha subunits of LFA 1 ( CD11a ) , Mac 1 ( CD11b ) and LeuM 5 ( CD11c ) , and their common beta subunit ( CD 18 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The three subsets shared a great deal of phenotypic similarity , expressing CD 2 , CD 7 , CD11b , CD 38 , CD45R , CD 18 , and the p 75 IL 2R on the majority of the cells in each subset . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Pretreatment of lymphocytes with mAb to the accessory molecules CD 2 , CD 3 , CD 4 , CD 8 , CD11b , or CD11c did not interfere with their response to HD cells . mAb to CD11a ( LFA 1 ) , the alleged ligand of ICAM 1 , inhibited the ICAM 1 but not the HD lectin mediated lymphocyte stimulation . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The receptor on human neutrophils ( polymorphonuclear leukocytes ) that mediates cellular adherence consists of two noncovalently associated subunits , designated alpha M ( Mac 1 alpha , Mol alpha , or CD11b ; Mr , 170 , 000 ) and beta ( Mac 1 beta , Mol beta , or CD 18 ; Mr , 100 , 000 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Inhibition of PMN attachment to IL 1 activated HEC was measured in a quantitative in vitro monolayer adhesion assay , after treatment with mAb directed to ELAM 1 ( mAb H18 / 17 ) , and to CD11a ( mAb L 11 ) , CD11b ( mAb 44 ) , CD11c ( mAb L 29 ) , and CD 18 ( mAb 10F12 ) , alone or in combination . ^^^ CD11 / CD18 directed mAb significantly blocked PMN adhesion to activated HEC ( anti CD11a , 40 + / 3 % ; anti CD11b , 34 + / 4 % ; anti CD 18 , 78+ / 6 % inhibition ) . ^^^ At this time , CD11 / CD18 dependent adhesive mechanisms predominated and a CD11c dependent mechanism became apparent ( anti CD11a , 67 + / 4 % inhibition ; anti CD11b , 45 + / 9 % ; anti CD11c , 26 + / 6 % ; anti CD 18 , 97 + / 1 % ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
It was assessed for the ability to activate the following proinflammatory functions in neutrophils isolated from the blood of healthy dogs : 1 ) morphological changes characteristic of chemotactic stimulation , which were assessed by phase contrast microscopy , 2 ) orientation of canine neutrophils in a gradient of cardiac lymph , which was assessed in Zigmond chambers , 3 ) the binding of monoclonal antibodies reactive with CD11b and CD 18 adherence glycoproteins , which was assessed by flow cytometry , and 4 ) adherence of canine neutrophils to monolayers of canine jugular vein endothelium , which was assessed in vitro by a visual assay . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Adherence was inhibited by monoclonal antibodies 60 . 1 and 60 . 3 , which are directed to functional epitopes on the CD11b and CD 18 polypeptides of the neutrophil membrane adhesion complex ( Mac 1 , Mo 1 ) . ^^^ Sulfhydryl reducing agents promote neutrophil adherence without increasing surface expression of CD11b / CD18 ( Mac 1 , Mo 1 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Antibodies directed towards integrin molecules ( LFA 1 alpha or beta chain , CD11a and CD 18 respectively ) reduced the adherence , others ( anti CR 3 or anti gp 150 / 95 , CD11b and c respectively ) did not influence it . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies against CD 2 , CD 4 , CD11a , CD 18 , LFA 3 , ICAM 1 , class 1 and class 2 major histocompatibility complex ( MHC ) inhibited T cell proliferation in this response , whereas antibodies against CD 8 , CD11b , CD11c and CD 16 had no effect . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
To determine if CD11b CD 18 has the ability to recognize ICAM 1 , human macrophages were plated on culture surfaces coated with purified ICAM 1 . ^^^ To determine the individual roles of CD11a / CD18 ( LFA 1 ) , CD11b / CD18 ( CR 3 , Mac 1 ) and CD11c / CD18 ( p 150 , 95 ) in adhesion of PDB stimulated PMN to unstimulated EC , mAb against the CD 11 chains were used . mAb against CD11a or CD11b each blocked adhesion of PMN to EC by approximately 50 % , but mAb against CD11c had no effect . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
MoAbs directed against epitopes on the alpha polypeptide of the CD11b ( Mol , MAC 1 ) heterodimer also inhibited PMN adherence to EC and to cell free surfaces induced by these agonists . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The leukocyte adhesion 90 kDa glycoprotein GP 90 ( antigen CD 18 ) is non covalently associated separately with cell surface glycoproteins GP 160 ( antigens CD11a , TA 1 , LFA 1 ) , GP 155 ( antigens CD11b , OKM 1 , MO 1 ) or GP 130 ( antigens CD 11c , Leu M 5 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The acquisition of monocyte cytotoxicity was associated with nearly a twofold increase in surface expression of three CD 18 bearing cell surface molecules ( CD11a , CD11b , CD11c ) . ^^^ The inhibition by anti CD 18 MAb could not be attributed to LFA 1 ( CD11a ) alone , suggesting that CR 3 ( CD11b ) and p 150 , 95 ( CD11c ) may also participate in monocyte mediated cytotoxicity . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The response to H2O2 closely resembled the adhesive response of U 937 cells to phorbol esters in its time dependency , requirement for extracellular Mg2+ and inhibition by cytochalasin B as well as inhibition by monoclonal antibodies against the leucocyte adhesion molecules CD11b and CD 18 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We have compared the requirements of the primary MLR and the responses of alloreactive , primed lymphocytes ( PL ) by inhibition studies using monoclonal antibodies ( MoAb ) directed against ( 1 ) adhesion molecules belonging to the CD 11 cluster of leucocyte antigens ( CD11a , LFA 1 ; CD11b , MAC 1 = CR 3 ; and CD11c , p 150 , 95 ) ; ( 2 ) various T cell related antigens ( CD 2 , CD 4 , CD 5 and CD 8 ) ; and ( 3 ) recombinant IL 1 beta . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Previous investigations of p 150 , 95 ( CD11c ) , the third member of the CD 18 membrane glycoprotein family that includes CR 3 ( Mac 1 or CD11b ) and LFA 1 ( CD11a ) , had demonstrated that solubilized p 150 , 95 bound to iC3b agarose in a manner similar to isolated CR 3 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
To assess the possible in vivo significance of these observations we studied the effect of GM CSF on CD11b , CD11a ( LFA 1 ) , and CD11c ( gp 150 , 95 ) expression on granulocytes from nine adult patients with sarcoma who were receiving GM CSF as part of a phase 1 trial . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Whereas noninfected U 937 cells expressed moderate amounts of lymphocyte function associated antigen 1 ( LFA 1 ) ( CD11a ) and minimal amounts of the C3bi receptor ( CD11b ) and p150 / 95 ( CD11c ) , U937 / HIV cells expressed moderate amounts of C3bi receptor and p150 / 95 and showed elevated expression of LFA 1 alpha ( CD11a ) and beta ( CD 18 ) chains . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Stimulation of human neutrophils by the chemoattractant formyl methionyl leucyl phenylalanine or the calcium ionophore A 23187 induced increments in the cell surface expression of the alpha subunits of Mo 1 ( CD11b ) and gp 150 , 95 ( CD11c ) and their common beta subunit ( CD 18 ) . ^^^ By subcellular fractionation and immunoprecipitation studies , we have found that CD11b , CD11c and CD 18 molecules were mainly localized in the membranes of tertiary granules , which were resolved from the specific and azurophilic granules as well as from the plasma membrane fraction . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We found that phorbol myristate acetate ( PMA ) , calcium ionophore ( A 23187 ) , and FMLP caused a three to sevenfold increase in surface expression of both CD11b ( alpha M ) and CD 18 ( beta ) as assayed by binding of MAbs 60 . 1 ( anti CD11b ) and 60 . 3 ( anti CD 18 ) . ^^^ Pretreatment of neutrophils with the anion channel blocking agent , DIDS ( 4 , 4 ' diisothiocyanostilbene 2 , 2 ' disulfonic acid ) , inhibited the increased surface expression of CD11b and CD 18 after stimulation by PMA , A 23187 , or FMLP and resulted in nearly complete inhibition of neutrophil aggregation . ^^^ We also observed that stimulation of granule depleted neutrophil cytoplasts by PMA , A 23187 , or FMLP induced aggregation and adherence to endothelial monolayers without increasing surface expression of CD11b or CD 18 . ^^^ Increased surface expression of CD11b / CD18 ( Mac 1 ) is not required for stimulated neutrophil adherence to cultured endothelium . ^^^ The mechanism whereby the human neutrophil membrane heterodimer , CD11b / CD18 ( Mac 1 , Mo 1 ) , mediates neutrophil adherence is not known . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
This antibody recognizes GP 90 ( CD 18 ) , a leukocyte surface glycoprotein which is separately and noncovalently associated to either GP 160 ( CD11a ) , GP 155 ( CD11b ) , or GP 130 ( CD11c ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Whether homotypic neutrophil aggregation depends on the quantitative increase of gp165 / 95 molecules ( Mac 1 , CR 3 ) recruited to the cell surface during activation was studied using mAb of the CD11b group that recognize distinct epitopes encoded by the alpha subunit of this glycoprotein . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
This receptor consists of two noncovalently associated subunits , designated alpha M ( Mac 1 alpha , CD11b ) ( Mr = 170 , 000 ) and beta ( Mac 1 beta , CDw 18 ) ( Mr = 100 , 000 ) , respectively , which are identical on normal and CML PMN . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
By immunofluorescence analysis , CD11b and CD 18 monoclonal antibodies specific for the 155 kd alpha polypeptide of Mo 1 ( gp 155 , 94 ) and the 94 kd beta peptide common to Mo 1 , LFA 1 ( gp 170 , 94 ) , and Leu M 5 ( p 150 , 94 ) ( surface molecules that promote leukocyte adhesion ) failed to bind to unstimulated and A 23187 calcium ionophore stimulated granulocytes or mononuclear cells of the affected dog as compared with strong specific binding to canine control cells . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In this study , the activation and functional state of neutrophils in the blood and synovial fluid of patients with rheumatoid arthritis were compared : mean density of neutrophil activation markers CD11b , CD 18 and L selectin was measured with a flow cytometer , and adhesion to chondrocytes using a fluorimetric assay . ^^^ For neutrophils of control and patient peripheral blood , fMet Leu Phe stimulation induced upregulation of CD11b ( resp p = 0 . 007 and p = 0 . 008 ) and CD 18 ( resp p = 0 . 005 and p = 0 . 01 ) . ^^^ In the synovial fluid , no significant increase in CD11b and CD 18 could be induced with fMet Leu Phe . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The following molecules were detected in patients before culture : CD 18 ( mean percentage 94 % ) , CD11a ( 94 % ) , CD11b ( 39 % ) , CD 54 ( 23 % ) , CD 58 ( 62 % ) , CD 44 ( Hermes 1 ; 82 % ) , CD 49 d ( VLA 4 ; 80 % ) , CD 25 ( 11 % ) and LECAM 1 ( 62 % ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
FACS analysis revealed the expression of SLex as well as other adhesion molecules such as L selectin , CD11a , CD11b and CD 18 on the cell surface of rat PMN . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The proportion of MPO+ cells slightly exceeded but clearly correlated with the proportion of cells positive for the granulomonocyte associated surface molecules CD11b ( Mac 1 ) , CD 15 ( LeX ) , CD 64 ( Fc gamma RI ) CD 66 , or CD 89 ( Fc alpha R ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Under these conditions , a concentration dependent increase in CD11b / CD 18 and lactoferrin release were observed only at a concentration between 0 and 30 % ( vol / vol ) dialysis effluent , while a concentration dependent increase in oxidase activity was seen at a concentration between 0 and 70 % ( vol / vol ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Both anti CD11a ( LFA 1 ) and anti CD11b ( MAC 1 ) therapy delay the onset and diminish the severity of experimental autoimmune encephalomyelitis . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Three criteria establish that the endogenous IgM expressing B cells belong to the B 1 cell lineage . ( 1 ) Endogenous IgM expressing B cells in B 6 Sp6 mice have the same localization pattern as B 1 cells from normal animals : they are enriched in the peritoneal cavity . ( 2 ) The endogenous IgM+ B cells have the phenotype of B 1 cells : the endogenous IgM+ peritoneal B cells express Mac 1 ( CD11b ) and low levels of IgD , and most also express CD 5 ( Ly 1 ) . ( 3 ) B 6 Sp6 BM poorly reconstitutes endogenous IgM+ B cells , just as adult BM from normal mice poorly reconstitutes B 1 cells . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Homotypic adhesion could be inhibited by mAb to CD11b , CD 18 and CD 54 , indicating an involvement of the beta 2 integrin cyto adhesion pathway . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies against the adhesion glycoproteins CD 18 , CD11b , intercellular adhesion molecule 1 ( ICAM 1 ) ( at 10 and 30 minutes ) , and L selectin ( at 10 minutes ) , but not P or E selectin , reduced I / R induced leukocyte adherence and emigration as well as albumin leakage . ^^^ Platelet leukocyte aggregates were formed in postischemic venules ; the number of aggregates was reduced by antibodies against P selectin , CD11b , CD 18 , and ICAM 1 , but not E or L selectin . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Furthermore , treatment of the BBECs with antibodies against CD11a ( LFA 1 ) , CD11b ( MAC 1 ) , or ICAM 1 significantly decreased SP induced adherence ( p < 0 . 01 all comparisons ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Studies with MoAbs against adhesion molecules on monocytes revealed that the binding of rIFN gamma stimulated monocytes , but not that of non stimulated monocytes , to rIL 1 alpha stimulated EC was inhibited by about 30 60 % with MoAbs against CD11a , CD11b , CD 18 , L selectin or CD 14 . ^^^ Flow cytometric analysis demonstrated that monocytes stimulated with 500 U / ml rIFN gamma for 24 h showed increased expression of CR 3 ( CD11b / CD18 ) , p 150 , 95 ( CD11c / CD18 ) and Fc gamma RI ( CD 64 ) ; the expression of LFA 1 ( CD11a / CD18 ) , L selectin , CD 14 and VLA 4 ( CD49d / CD29 ) did not change or was slightly reduced . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
This effect appeared to be restricted to CD11b ( CR 3 ) and CD11c ( gp 150 95 ) , because CD11a ( LFA 1 alpha chain ) was not modified . beta 2 Adrenoceptor stimulation was also found to potentiate the effect of IL 4 on CD11b , CD11c , and CD 18 expression . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Adherent PMNs appeared morphologically activated and flow cytometric analysis of adherent PMNs confirmed activation because CD11b and CD 18 surface expression was upregulated ( 100 % and 27 % , respectively ) , whereas L selectin was downregulated ( 55 % ) compared with control nonadherent PMNs . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We herein report a case of acute myeloid leukaemia ( AML , FAB : M 0 ) who showed upregulation of T lymphoid antigens ( CD 2 , CD 7 ) and adhesion molecules ( CD11a , CD11b , CD 18 ) on leukaemic cells after in vivo administration of granulocyte colony stimulating factor ( G CSF ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
To identify the molecules involved in this adhesion dependent degranulation , we have investigated the effects of mAbs ( mAb ) against beta 2 integrins . mAb reactive with CD 18 markedly inhibited the eosinophil adhesion and degranulation induced by PAF and human rGM CSF . mAb reactive with CD11b also moderately inhibited the adhesion and degranulation . ^^^ Superoxide production induced by human rGM CSF and PAF was also abolished by the treatment of cells with anti CD 18 mAb . mAb against CD11b and CD 18 had little effect on degranulation and superoxide production induced by PMA . ^^^ CD11b / CD18 ( Mac 1 ) is required for degranulation of human eosinophils induced by human recombinant granulocyte macrophage colony stimulating factor and platelet activating factor . ^^^ These results indicate that CD11b / CD18 ( Mac 1 ) dependent cellular adhesion plays an important role in the degranulation and superoxide production of eosinophils induced by human rGM CSF and PAF , and that these mechanisms may be employed in vivo where eosinophils contact with stromal cells and / or proteins . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The density ( expressed as mean fluorescence intensity ) of CD11b on monocytes and polymorphs was increased ( P < 0 . 005 ) , as was CD11c ( P < 0 . 005 ) and CD 18 ( P < 0 . 05 ) on polymorphs . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The expression levels of CD11b , CD 13 , CD 14 , CD 16 , and CD 18 antigens and L selectin ( TQ 1 and Leu 8 epitopes ) on neutrophils and monocytes from 15 normal individuals were determined and compared with a previously used method in which the leukocytes were fixed and the erythrocytes lysed before analysis . ^^^ Significant differences for the apparent expression of CD11b , CD 18 , and L selectin were observed between the two methods . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The enhanced phagocytosis was inhibited by Abs to CD 18 or CD11b , suggesting that P selectin alters beta 2 integrin function . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Dibutyryl cAMP induced eosinophilic differentiation of EoL 1 cells from 6 9 days after the start of culture with down regulation of CD11a , CD 18 , and CD 49 expression and up regulation of CD11b expression . ^^^ We examined the effect of dibutyryl cAMP ( dbcAMP ) on the expression of LFA 1 ( CD11a / CD18 ) , Mac 1 ( CD11b / CD18 ) , and VLA 4 ( CD49 / CD29 ) and on eosinophilic differentiation of a human leukemia cell line , EoL 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Mononuclear phagocyte ( MO ) activation was associated with rapid production of TNF alpha , down regulation of the glycophosphatidylinositol linked protein CD 14 ( the key MO receptor for complexes of LPS and LPS binding protein responsible for intracellular signaling ) , and down regulation of the related LPS binding proteins CD11b and CD 18 . ^^^ Thus , APC inhibited the production of TNF alpha and prevented down regulation of membrane CD11b , CD 14 , and CD 18 , but had no effect on up regulation of MHC class 2 , ICAM 1 , or IL 2R , down regulation of MO expression of another glycophosphatidylinositol linked protein , CD 59 , or production of reactive oxygen intermediates . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
CMC were not recognized by MoAbs to lymphocyte function associated antigen 2 ( LFA 2 ; CD 2 ) , T cell receptor ( TcR ; CD 3 ) , T 4 antigen ( CD 4 ) , LFA 1 alpha chain ( CD11a ) , C3biR alpha chain ( CD11b ) , CR 4 alpha chain ( CD11c ) , LPS R related Ag ( CD 14 ) , 3 FAL / x hapten ( CD 15 ) , Fc gamma RIII ( CD 16 ) , lactosylceramid ( CDw 17 ) , the B cell antigen CD 19 , or CR 1 ( CD 35 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
All three chemokines tested induced increased expression of the alpha chains of two members of beta 2 family of integrins , CD11b and CD11c , and their common beta chain ( CD 18 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Specific monoclonal antibodies ( mAbs ) to CD11b and to CD 18 were used in the present study to evaluate the contribution of each chain to these processes . ^^^ All four anti CD 18 mAbs but none of five anti CD11b mAbs dose dependently blocked PMN phagocytosis of zymosan . ^^^ Nevertheless , all anti CD11b mAbs and all anti CD 18 mAbs significantly inhibited zymosan stimulated LTB 4 release in a dose dependent manner . ^^^ Thus zymosan stimulated LTB 4 release is initiated by signals from both chains of the CD11b / CD18 integrin , whereas only CD 18 is essential for phagocytosis . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We have compared the mean fluorescence intensity ( MFI ) values for CD11b , CD 18 , and L selectin ( Leu 8 and TQ 1 epitopes ) on human peripheral blood leucocytes , using these two approaches . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The cells expressing a high level of D factor receptor were induced to differentiate by D factor ; about 60 % of the cells exhibited the ability to reduce nitroblue tetrazolium and expression of the differentiation antigen Mac 1 ( CD11b ) on the cell surface increased . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Protective effects of mAb were also reflected in reductions of retrievable neutrophils in BAL fluids . mAb to rat CD11b and CD 18 but not to rat CD11a suppressed in vitro production of TNF alpha by immune complex stimulated rat alveolar macrophages . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Initial localization of epitopes was accomplished using chimeric proteins constructed by splicing fragments of cDNAs encoding the alpha subunit of LFA 1 ( CD11a ) and the alpha subunit of the closely related leukocyte integrin , Mac 1 ( CD11b ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Triggering via CD 40 using NIH3T3 CD 40 ligand transfectant ( CD40LT ) cells increased ( > 30 % ) cell surface CD 80 , CD 18 , CD11a , CD11b , and CD11c expression on MM cell lines . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Certain antibodies directed against CD 18 , CD11b and CD11c inhibited HIV 1 induced syncytium formation but not entry , in agreement with previous reports . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The following parameters were studied : flow cytometry evaluation of surface CD 32 , CD 16 , CD11b and CD 18 by means of a whole blood method ; whole blood phagocytosis by means of a flow cytometric assay ; whole blood chemiluminescence using opsonized zymosan as a stimulus . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Indirect immunofluorescence and Western blot analysis with monoclonal antibodies OX 42 and ED 9 showed that HM phi from cholestatic rats expressed MAC 1 ( CD11b ) and the LPS receptor CD 14 , respectively . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
To investigate cardiac inflammatory responses in patients with acute MI , we examined the cardiac release of cytokines , the expression on neutrophils of the beta 2 integrin Mac 1 ( CD11b / CD18 ) and L selectin ( CD62L ) , and the cardiac release of thrombomodulin as a marker of endothelial injury . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Similarly , eosinophils exposed to human IgG immobilized to plates adhered and degranulated ; in addition , adherence and subsequent degranulation were inhibited by mAbs to CD 18 and CD11b , but not by mAb to CD 29 , suggesting an important role of beta 2 integrin for these responses . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The histograms showed a population of larger cells expressing CD 18 and CD11b , distinct from the smaller cells which did not express CD11b . fMLP caused an increase in intracellular calcium ( measured spectrophotometrically ) that was inhibited by the calcium chelator BAPTA . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
METHODS Expression of CD11b , CD 14 , CD 18 , and CD 64 on monocytes and neutrophils was assessed by flow cytometry . ^^^ There was no correlation between cytokine concentrations and the expression of CD11b , CD 14 , CD 18 , and CD 64 on patients ' phagocytes . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Prick test weals ( of 10 min duration ) to common inhalant allergens showed only a minor increase of CD 18 , whereas in a case of cold urticaria CD11b and CD 18 molecules were increasingly upregulated within the first 30 min after elicitation of the lesions . ^^^ Skin test sites in delayed pressure urticaria , and urticarial lesions ( > 6 h duration ) of acute and chronic recurrent urticaria also showed marked upregulation of CD11b and CD 18 , and to a lesser extent of CD11a , but this did not strongly correlate with the intensity of the mixed cellular infiltrate . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Antibodies to CD 18 and CD11b reduced migration by 70 to 79 % , suggesting that CD11b / CD18 ( Mac 1 ) was acting as the neutrophil receptor for ICAM 1 in this process . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In vitro incubation with these cytokines for 24 hours significantly increased eosinophil membrane expression of CD 18 and CD11b compared with culture in medium alone or eosinophils obtained by bronchoalveolar lavage . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
To delineate the adhesion proteins involved in the cell cell interactions , assays were performed in the presence of specific blocking monoclonal antibodies to eosinophil CD 18 , CD11a , or CD11b , and REC ICAM 1 molecules . ^^^ In contrast , antibodies to CD 18 , CD11a , and CD11b significantly decreased ( P < 0 . 01 ) eosinophil adhesion , thus demonstrating pivotal roles for the CD11 / CD18 ( beta 2 ) integrins , but not necessarily for ICAM 1 , in interactions between the REC and eosinophils . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In rectal mucosa , CR 3 was detected in the surface and crypt epithelial cells by immunohistology , using MoAbs to CD 18 and CD11b in 10 out of 15 specimens . ^^^ RNA transcripts encoding both CD11b and CD 18 were also demonstrated in surface and crypt epithelial cells by in situ hybridization . ^^^ Although CD11b was detected in the epithelial cells in three out of the 14 cervicovaginal specimens , we were unable to detect CD 18 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
This study examined the relationship between exercise induced changes in the concentration of circulating immunocompetent cells and their surface expression of adhesion molecules : L selectin ( CD62L ) and three beta 2 integrins [ LFA 1 ( CD11a / CD18 ) , Mac 1 ( CD11b / CD18 ) , and p150 / 95 ( CD11c / CD18 ) ] . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The beta 2 integrin consists of lymphocyte function antigen 1 ( LFA 1 , CD11a / CD18 ) which binds to the major ligand intracellular adhesion molecules 1 and 2 ( ICAM 1 and ICAM 2 ) , Mac 1 ( CD11b / CD18 , ligand iC3b ) and p 150 , 95 ( CD11c / CD18 , ligand iC3b ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Down regulation of CD11b and CD 18 expression in atherosclerotic lesion derived macrophages . ^^^ We have compared CD11b and CD 18 expression of peripheral blood monocytes from normal rabbits ( NM ) to atherosclerotic lesion derived macrophages ( AthM ) by anti CD11b and anti CD 18 antibody staining , followed by flow cytometry and immunohistochemical staining . ^^^ Our results indicated that NM showed a strong cell surface expression of CD11b and CD 18 . ^^^ The less granular and smaller AthM showed little anti CD11b or anti CD 18 antibody staining , indicating very little CD11b or CD 18 antibody staining , indicating very little CD11b or CD 18 expression . ^^^ The more granular and larger cells showed surface expression of both CD11b and CD 18 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Room air was controlled and hypoxic cells were labeled with fluorescent monoclonal antibodies to integrins CD 18 and CD11b or to the 55 kDa TNF alpha cell surface receptor ( TNFR ) . ^^^ Physical hypoxia increased expression of both CD11b and CD 18 over time and augmented their LPS induced up regulation . ^^^ Isolated chemical hypoxia did not change neutrophil expression of CD11b or CD 18 , but partially inhibited neutrophil CD11b and CD 18 up regulation by LPS . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We show that IFN beta downregulates both basal and IFN gamma induced expression of VLA 4 ( very late activation antigen 4 ) antigen on monocytes , but has no effect on the expression of CD11a , CD11b , CD11c , L selectin , CD 18 , ICAM 1 , beta 1 integrin or CD 44 on monocytes or ICAM 1 on EAhy 926 cells . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Hematopoietic cell type dependent regulation of leukocyte integrin functional activity : CD11b and CD11c expression inhibits LFA 1 dependent aggregation of differentiated U 937 cells . ^^^ These results confirm the role of CD11b / CD18 and CD11c / CD18 in myeloid cell adhesion and spreading and suggest that the CD11b / and CD11c / CD18 mediated recognition of substrate bound ligands competes or interferes with LFA 1 dependent intercellular adhesion . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Intracellular calcium content was monitored in real time with the calcium indicator , indo 1 , after cross linking Fc gamma RI , Fc gamma RII , CD11b , CD11c , or CD 18 molecules , or addition of interleukin 1 alpha , IL 1 beta , interferon gamma , bradykinin , substance P , or FMLP . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The MoAb OKM 1 against CD11b , which blocks fibrinogen binding to CD11b / CD18 as well as the MoAbs IOT 16 and IOT 18 directed against CD11a and CD 18 , respectively , had no effect . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
CD11b / CD18 ( Mac 1 ) is a leukocyte integrin that plays a critical role in neutrophil adhesion and the initiation of acute inflammatory responses . ^^^ Several Mac 1 blocking mAbs bind to the A domain of CD11b , a approximately 200 amino acid region in the N terminal portion of the protein that is involved in ligand binding and Mac 1 functional activity . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Normal blood basophils and KU 812 cells were found to express C5aR ( CD 88 ) , membrane cofactor protein ( CD 46 ) , decay accelerating factor ( CD 55 ) , and membrane attack complex inhibitory factor ( CD 59 ) , as well as the previously recognized CR 1 ( CD 35 ) , CR 3 alpha ( CD11b ) , CR 4 alpha ( CD11c ) , and CR3 / 4 beta ( CD 18 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
A high level of expression of the G CSFR did not promote or suppress cellular proliferation or initiate differentiation ; however , exposure of transfected cells to G CSF in suspension culture caused a large percentage of the population to enter a differentiation pathway , as determined by two markers of the mature state , the ability of cells to reduce nitroblue tetrazolium ( NBT ) and to express the differentiation antigen Mac 1 ( CD11b ) on the cell surface . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The beta 2 integrins are composed of a common 95 kD beta subunit ( CD 18 ) and one of three possible alpha subunits : CD11a , CD11b , or CD11c . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Preincubation of HUVEC with antibodies against E selectin ( 10 micrograms / ml ) significantly inhibited ( P < 0 . 02 ) the binding of radiolabelled BDC to activated HUVEC ( 32 . 2 + / 1 . 3 % ) whereas preincubation of BDC with antibodies against CD 54 , CD 18 , CD11b , CD11c and Sialyl Lewis 10 did not produce any significant inhibition . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
However , no statistically significant differences were found between PMNL from DS children and those from controls in phagocytosis , oxidative burst , and expression of the markers CD11a , CD11b , CD 16 , and CD 18 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Moreover , TNF alpha showed a co distribution with ICAM 1 expressed in the membranes of hepatocytes and with the occurrence of CD11b positive polymorphonuclear leukocytes ( neutrophils ) suggesting a possible major role of the beta 2 integrin Mac 1 as a ligand for ICAM 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Treatment of leukocytes with phorbol myristate acetate ( PMA ) caused significant increases in the expression of adhesion molecules , CD11a , CD11b , CD11c , and CD 18 , on the surface of the leukocytes . ^^^ Monoclonal antibodies against CD11a , CD11b and CD 18 , and ICAM 1 , an adhesion molecule in the side of endothelial cells , abolished significantly the endothelial cell injury induced by PMA stimulated leukocytes . ^^^ The monoclonal antibodies against CD11a , CD11b , CD 18 , and ICAM 1 also showed inhibitory effects on the increase in intracellular fluorescence intensity of the endothelial cells exposed to PMA stimulated leukocytes . ^^^ Both the early increase in peroxide production and late cell lysis were abolished by specific antibodies against CD11a , CD11b , CD 18 , and ICAM 1 , but not CD11c . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
CD 18 cDNA carrying the abnormalities were cotransfected with normal CD11a or CD11b cDNA into COS cells . ^^^ Expression of the LFA 1 ( CD11a / CD18 ) and Mac 1 ( CD11b / CD18 ) antigens on COS cells was not detected , suggesting that these two mutations are sufficient to account for LAD . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
According to previous findings , basophils express CD 9 ( p 24 ) , CD11a ( LFA 1 alpha chain ) , CD11b ( C3biR ) , CD11c ( CR 4 ) , CD 13 ( aminopeptidase N ) , CDw 17 ( lactosylceramide ) , CD 18 ( beta chain of beta 2 ) , CD 25 ( IL 2R alpha chain ) , CD 26 ( dipeptidylpeptidase ) , CD 31 ( PECAM ) , CD 35 ( CR 1 ) , CD 38 ( T 10 ) , CD 43 ( leukosialin ) , CD 44 ( Pgp 1 ) , CD 45 ( pan leukocyte Ag ) , and CD 63 ( basophil activation Ag ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In addition , these cells were weakly positive for CD11b ( Mac 1 ) and CD 54 ( ICAM 1 ) , but were negative for lymphocyte specific CD11a ( LFA 1 ) and CD11c ( p 150 , 95 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies recognizing CD 18 and CD11b subunits of Mac 1 inhibited adhesion of PMN to collagens by an order of magnitude more effectively than to all noncollagenous substrates . ^^^ The leukocyte integrin Mac 1 ( CD11b / CD18 ) contributes to binding of human granulocytes to collagen . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The beta subunit gene ( CD 18 ) is constitutively expressed on all leukocytes , however , the alpha subunit genes for LFA 1 , Mac 1 and p 150 , 95 ( CD11a , CD11b and CD11c , respectively ) show cell and developmental stage specific expression . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Immunohistochemistry was performed using monoclonal antibodies against macrophage markers ( ED 1 , CD 4 , RT 1 B , and ED 2 ) , chains of the beta 2 integrins ( CD 18 , CD11a , and CD11b ) , T and B cell markers ( CD 8 , T cell receptor , interleukin 2 receptor , and MRC OX 33 ) , and markers of mesangial cell proliferation ( proliferating cell nuclear antigen , alpha smooth muscle actin ) . ^^^ Second , the presence of CR 3 ( i . e . , CD11b and CD 18 ) and LFA 1 ( i . e . , CD11a and CD 18 ) on the macrophage infiltrate indicates that both ligands are important for cells to enter the mesangium and periglomerular areas . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
GVH also induced MAC 1 ( CD11b ) expression on a subset of CD8+ lymph node T cells , but MAC 1 was not up regulated on CD8+ IEL in this situation . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Surface expression of the beta 2 integrin chains ( CD11a , CD11b , CD11c and CD 18 ) , and the beta 1 integrin chains ( CD49b , CD49d , CD49f and CD 29 ) , as well as that of members of the immunoglobulin superfamily ( CD 2 , CD 54 , CD 56 and CD 58 ) , were analyzed by one or two color flow cytometry . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The CR 3 is composed of the alpha ( CD11b ) and beta ( CD 18 ) subunits , which contain a short intracytoplasmic domain devoid of catalytic activity . ^^^ For this purpose CR 3 was immunoprecipitated from radiolabeled bone marrow macrophages using a combination of anti CD11b and anti CD 18 mAbs . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Using flow cytometry analysis , the % s of CD 18 , CD11a and CD11b positive cells and the absolute numbers of fluorescent molecules were determined . ^^^ The levels of CD 18 and CD11b expressed by CF PMN were higher than those expressed by PB PMN and the difference was significant for CD11b ( p < 0 . 001 ) . ^^^ In this study , we assessed the LFA 1 ( CD18 / CD11a ) and CR 3 ( CD18 / CD11b ) expression on peripheral polymorphonuclear leukocytes ( PB PMN ) and crevicular fluid polymorphonuclear leukocytes ( CF PMN ) , by subjects with a healthy periodontium ( n = 7 ) , gingivitis ( n = 8 ) , early onset periodontitis ( n = 17 ) and adult periodontitis ( n = 8 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Injection of anti TNF monoclonal antibody R 6 over three days into rabbits with AIA reduced joint swelling and leucocyte infiltration into joint fluid and decreased the expression of CD11b and CD 18 on cells in the joint fluid . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In the present study we investigated the membrane expression of selectin ligands ( CD15 / Le ( 10 ) , CDw65 / VIM2 , CD15s / sLe ( 10 ) , beta 2 integrins ( CD11a / LFA 1 , CD11b / Mac 1 ) and CD 45 phosphatase isoforms ( CD45RA , CD45O ) on leukaemic cells from 28 patients with acute myeloid malignancies cultured with and without all trans retinoic acid ( ATRA ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Compared to regular monocytes , mature monocytes had lower levels of the high affinity Fc gamma receptor 1 ( CD 64 ) , complement receptor 3 ( CD11b ) , CD45RO and higher levels for HLA DR , LFA 1 ( CD11a / CD18 ) , interleukin 2 receptor ( CD 25 ) , CD45RA and the Fc gamma receptor 3 ( CD 16 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
CD11b is the alpha chain of the Mac 1 integrin and is preferentially expressed in myeloid cells ( neutrophils , monocytes , and macrophages ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Beta 2 ( CD 18 ) mutations abolish ligand recognition by 1 domain integrins LFA 1 ( alpha L beta 2 , CD11a / CD18 ) and MAC 1 ( alpha M beta 2 , CD11b / CD18 ) . ^^^ Recombinant alpha L beta 2 ( LFA 1 , CD11a / CD18 ) and alpha M beta 2 ( MAC 1 , CD11b / CD18 ) were expressed on COS cells , and function was assessed by adhesion to ICAM 1 or iC3b , respectively . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
During the third trimester fetal expression of CD 18 , CD11b , and CD11c on neutrophils and CD11b on monocytes was lower , and maternal expression of CD11a on neutrophils and CD 18 , CD11a and CD11c on monocytes was higher than were the control adult values . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Since TMEV induced demyelinating lesions are infiltrated with macrophages and a number of other persistent viruses show near exclusive tropism for these phagocytic cells , we used two color immunofluorescent staining with conventional and confocal microscopy to colocalize TMEV to cells that stain with monoclonal antibodies ( MOMA 2 ) [ unknown antigen ] , Mac 1 [ CD11b ] , FA 11 [ CD 66 ] , and 2F8 [ scavenger receptor ] ) to macrophages in BeAn infected SJL mice . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The extract induced adhesion was significantly diminished by monoclonal antibodies ( MAb ) directed against either CD11a , CD11b , or CD 18 on neutrophils , and by MAbs against intercellular adhesion molecule 1 ( ICAM 1 ) , but not E or P selectin , on endothelial cells . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Cross linking of CD11b or CD 18 signals release of localized Ca2+ from intracellular stores in neutrophils . ^^^ Cross linking of antibodies bound to CD11b or CD 18 on the neutrophil surface produced a large Ca2+ transient ( max . approximately 500 nM ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Like IL 4 , it enhanced the expression of CD11b , CD11c , CD 18 , CD 29 , CD49e ( VLA 5 ) , class 2 MHC , CD 13 , and CD 23 , whereas it decreased the expression of CD 64 , CD 32 , CD 16 , and CD 14 in a dose dependent manner . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
To evaluate the role of the cytoplasmic tails of CD11b and CD 18 in ligand binding and internalization of the human complement receptor type 3 ( CR 3 , CD11b / CD18 ) , wild type , and truncation mutants of CD11b and CD 18 cDNA were expressed in COS fibroblastoid cells and assayed for surface membrane expression , ligand binding , and internalization . ^^^ Truncated CD11b ( alpha 1119T ) and CD 18 ( beta 728T ) subunits retaining , respectively , two and five residues of the putative cytoplasmic tails were generated by in vitro mutagenesis . ^^^ Truncation of the cytoplasmic tail of CD 18 but not of CD11b markedly reduced coated pit internalization of CR 3 , an effect also produced by a single F754A mutation involving one of two putative NPXF internalization signals in CD18 . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Induction of neutrophil respiratory burst by tumour necrosis factor alpha ; priming effect of solid phase fibronectin and intervention of CD11b CD 18 integrins . ^^^ The O 2 production , but not the cell adherence to FN , was completely inhibited by two MoAbs against CD 18 and by a MoAb against CD11b , suggesting the involvement of CD11b CD 18 integrins in the neutrophil oxidative response . ^^^ The O 2 production by adherent neutrophils could be inhibited by anti CD11b and anti CD 18 MoAbs only when the MoAbs were present both during the induction of adherence and during the subsequent exposure of FN bound cells to TNF alpha . ^^^ The incapacity of MoAbs , added to neutrophils during the induction of adherence , to modify the characteristics of the subsequent neutrophil response to TNF alpha suggests that the FN mediated cell priming is independent of the interaction of CD11b CD 18 integrins with the FN substrate . ^^^ The results are consistent with the intervention of three classes of cell receptors in the TNF alpha induced oxidative burst of neutrophils plated on FN : ( 1 ) neutrophil FN binding sites , distinct from CD11b CD 18 and responsible for the cell priming ; ( 2 ) CD11b CD 18 integrins , absolutely required for permitting the cell triggering ; and ( 3 ) TNF alpha receptors , responsible for switching on a rapid cell response in primed cells . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
A sandwich enzyme linked immunosorbent assay , in which the CR 3 heterodimers in PMN lysates were captured by MoAb to the beta chain , CD 18 ( R15 . 7 ) , then detected with a biotinylated MoAb to the alpha chain , CD11b ( anti Mac 1 ) , showed that neonatal PMN lysates contain about 66 % of adult PMN levels of CR 3 ( P < 0 . 03 ; n = 6 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The monoclonal antibodies against CD11a , CD11b , CD 18 , and ICAM 1 showed almost complete inhibition of the increase in intracellular peroxide levels of the endothelial cells exposed to PMA stimulated leukocytes . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
However , mAbs against CD11b or CD 18 on PMN blocked PMN adhesion to ozone exposed TEC by approximately 55 and 80 % , respectively . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
On the other hand , conditioned medium from hypoxia incubated HUVEC failed to activate PMN , as determined by the lack of PMN calcium concentration increase , the failure of superoxide anion production enhancement , as well as the absence of effects on the integrin CD 18 , CD11a , and CD11b expression . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Two inhibitors of tyrosine kinases with differing modes of action , erbstatin and herbimycin A , were found to inhibit the expression of CD11b and CD 18 stimulated by chemotactic factors ( fMet Leu Phe or leukotriene B 4 ) or growth factors ( tumor necrosis factor alpha ) . ^^^ Augmentation of tyrosine phosphorylation by inhibiting tyrosine phosphatases using hydroperoxyvanadate led to an increased surface expression of CD11b and CD 18 and enhanced the adhesion of neutrophils to endothelial cells . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We tested fluorometrically the expression of neutrophil membrane molecules ( CD11b , CD11c , CD 18 ) and neutrophil oxidative burst using a chemiluminescence ( CL ) generation system . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The leukocyte integrins consist of a common beta 2 chain ( CD 18 ) covalently linked to one of three different alpha chains ( CD11a , CD11b , CD11c ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Several in vivo studies indicate that not only monoclonal antibodies ( mAbs ) directed against the common beta subunit ( CD 18 ) but also to the individual alpha subunits ( CD11a , CD11b ) can effectively inhibit different types of inflammation . ^^^ In this study we report that in the adjuvant arthritis ( AA ) alpha CD11a , alpha CD11b , or even alpha CD 18 treatment could not prevent disease development . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Monocyte adhesion to serum adsorbed surfaces is inhibited by mAbs to the leukocyte integrin beta subunit , CD 18 ( mAbs 60 . 3 and MHM 23 ) , and partially inhibited by a mAb to the alpha subunit , CD11b ( mAb 60 . 1 ) , suggesting adhesive interactions between adsorbed C3bi ( the hemolytically inactive form of the C3b fragment ) and the leukocyte integrin CD11b / CD18 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Function blocking monoclonal antibodies ( MAbs ) to the integrin beta 2 subunit ( CD 18 ) , including Fab ' fragments and alpha M ( CD11b ) blocked > 70 % of attachment , whereas MAbs to the beta 1 integrin subunit reduced attachment by < 30 % . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Leukocyte CD11b and CD 18 expression are increased in blood salvaged for autotransfusion . ^^^ To investigate whether activated leukocytes are present in salvaged blood , we measured complete blood counts and quantified the surface expression of the leukocyte adhesion receptors CD11b and CD 18 in salvaged blood and arterial blood from six male patients undergoing elective abdominal aortic aneurysm repair . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
When these clones were examined immediately after their establishment , expression of c jun was accompanied by the appearance of a mature phenotype in many clones , as measured by the reduction of nitroblue tetrazolium and by the expression of Mac 1 ( CD11b ) , a cell surface marker on differentiated cells . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We tested the promoter sequences from the leukocyte integrin CD11a ( LFA 1 ) , CD11b ( Mac 1 ) , and CD 18 subunits in retroviral vectors to express a reporter gene , adenosine deaminase , in the human leukocyte cell lines K 562 and HL 60 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Indirect immunoperoxidase staining patterns indicated that intratracheal challenge with beads activated BAL cells and upregulated expression of CD11b , ICAM 1 , and CD 18 antigenic markers on macrophages and neutrophils when compared with BAL cells from saline controls . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Radiodetoxified endotoxin induced tolerance alters monocyte but not neutrophil CD11b and CD 18 expression in response to lipopolysaccharide . ^^^ Peripheral leukocytes were obtained 1 hour after injection and were analyzed for CD11b and CD 18 expression by flow cytometry . ^^^ Tolerance to subsequent LPS challenge is associated with an abrogation of the reduced peripheral monocyte CD11b and CD 18 expression observed in native LPS stimulated mice but is not associated with changes in polymorphonuclear leukocyte CD11b and CD 18 expression . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The phenotype of splenic and peritoneal B 1 cells in normal BALB / c and BAB / 25 mice is essentially identical with the exception that all peritoneal B 1 cells express CD11b ( Mac 1 ) and some lack CD43 / S7 and heat stable Ag ( as detected by the mAb 53 10 ) expression . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Immunohistochemistry using the anti CD11b antibody ( Mac 1 ) was used to localize and quantify maternal inflammatory cells present at the fetoplacental interface . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Antibodies to CD 18 ( R15 . 7 ) or CD11b ( CL 44 and 60 . 1 ) reduced the incremental production of O 2 by 76 , 71 and 77 % , respectively . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Previously , it was shown that Oncoimmunin M ( OI M ) , a recently identified tumor cell derived 36 kDa protein , is able both to inhibit the proliferation of the human promyelocytic leukemic cell line HL 60 while maintaining viability in culture and to induce a bimodal distribution of CD11b , the alpha chain of the integrin MAC 1 , on the cell surface ( Packard , B . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The characteristics of CD 18 , CD11b , CD 4 , CD 8 and CD 3 antigen expression detectable by ICO monoclonal antibodies in the blood of hemoblastosis patients ] . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Surface expression of the beta 2 integrin chains ( CD 18 , CD11a , CD11b , CD11c ) , as well as that of members of the immunoglobulin superfamily ( CD 2 , CD 54 , CD 56 , CD 58 ) , were analysed by one or two colour flow cytometry . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
After treatment with PIF , Mac 1 Ag ( CR 3 or CD11b ) becomes almost undetectable on the cell surface by immunofluorescence staining using the mAbs D 12 and BEAR 1 , whereas staining with the LM2 / 1 anti Mac 1 mAb proved that Mac 1 is still present on the cell surface , thus , indicating a possible conformational change in Mac 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
MoAbs used were directed at myeloid ( CD11b , CD 13 , CD 14 , CD 15 , CD 33 , CD 41 ) , lymphoid ( CD 2 , CD 3 , CD 7 , CD 9 , CD 10 , CD 19 ) , or stem cell ( HLA DR , CD 34 , c kit receptor ) antigens , as well as the leukocyte integrins CD 18 and CD49e , and the transferrin receptor CD 71 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We then used flow cytometry to measure expression of the four membrane complement regulatory molecules , decay accelerating factor ( DAF ; CD 55 ) , complement receptor 1 ( CR 1 ; CD 35 ) , membrane cofactor protein ( MCP ; CD 46 ) and CD 59 ; two adhesion molecules of the integrin family LFA 1 ( alpha chain , CD11a ) , complement receptor 3 ( CR 3 ; alpha chain , CD11b ) , and their common beta chain ( CD 18 ) ; the major receptor for immune complexes Fc gamma RIII ( CD 16 ) , and the leucocyte common antigen tyrosine phosphatase ( L CA ; CD 45 ) . ^^^ PMN from RA expressed significantly less of all the complement C 3 convertase regulators ( CD 55 , CD 46 , CD 35 ) , all the adhesion molecules ( CD11a , CD11b , CD 18 ) and the phosphatase CD 45 than did blood PMN from age and sex matched control individuals . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
At the end of each period , the following parameters were determined during a hemodialysis session : C5a concentration by enzyme immunoassay and the neutrophil expression of CD11b , LFA 1 ( CD11a / CD18 ) , and the antigen recognized by MoF 11 ( MoF 11 Ag ) , a monoclonal antibody that recognizes activated neutrophils , by immunofluorescence flow cytometry . ^^^ Hemodialysis with complement activating membranes , such as cuprophane , induces neutropenia and expression of the granulocyte adhesion receptor Mac 1 ( CD11b / CD18 ) , while hemodialysis with noncomplement activating membranes does not . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The effects of the cytokine tumour necrosis factor alpha and the calcium ionophore A 23187 upon CD11a , CD11b , CD11c and CD 18 leucocyte membrane expression was analysed in whole blood using monoclonal antibodies and flow cytometry . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Engagement of the integrin CD11b / CD18 ( alpha M beta 2 , Mac 1 , CR 3 ) on cells of monocyte ( Mo ) lineage has recently been demonstrated to enhance synthesis and surface expression of the integral plasmalemma receptor tissue factor . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
After staining with specific fluorescent monoclonal antibodies , flow cytometric analysis was performed to evaluate the expression of CD11b ( = Mac 1 , CR 3 , or C3bi receptor ) , CD11a ( = leukocyte function antigen 1 or LFA 1 , or gp 180 / 95 ) , CD 54 ( = intercellular adhesion molecule 1 or ICAM 1 ) , and CD 45 ( = leukocyte common antigen ) on circulating leukocytes . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The expression of the Leu CAM proteins ( CD11a , CD11b , CD11c , CD 18 ) was also evaluated during the culture period . ^^^ The adhesion molecules CD11b alpha chain and CD 18 beta chain were strongly induced on the U 937 cells with a maximal expression of the CD11b on the cells cultured with either M CSF + GM CSF or the combinations of IL 6 and IFN gamma with M CSF + GM CSF . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In light of the ability of CD 18 integrins to participate in leukocyte adherence ( and thereby migration ) , we examined the role of the integrin CD11b / CD18 in NTN using OX 42 , a monoclonal antibody directed against rat CD11b . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The results in this study suggest that the CD 18 epitope , recognized by 60 . 3 , mediates both adhesion and migration of PMN while the epitope on CD11b recognized by the antibody Mo 1 is restricted to adhesion . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies to the carbohydrate structure Lewis ( 10 ) stimulate the adhesive activity of leukocyte integrin CD11b / CD18 ( CR 3 , Mac 1 , alpha m beta 2 ) on human granulocytes . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Expression of LeuCAM on freshly isolated cells was studied using the peroxidase antiperoxidase method with monoclonal antibodies ( MoAb ) detecting CD11a , CD11b , CD11c , and CD 18 . ^^^ Compared with nonsmokers , the samples from SA and IPF patients contained an increased number of AM expressing CD11a , CD11b , CD11c , and CD 18 ( all p < 0 . 008 ) , which was correlated to the number of AM / ml BAL ( p < 0 . 008 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We therefore determined neutrophil integrin expression under various experimental conditions using a Fluorescence Activated Cell Sorter ( FACS ) after the cells had been labelled with fluorescent conjugated antibodies to the integrin subunits CD11a , CD11b and CD 18 . ^^^ Levels of labelled CD11b and CD 18 increased after activation with the chemotactic peptide formyl methionyl leucyl phenylalanine ( fMLP ) in a dose and time dependent manner , but CD11a did not , indicating that CD11a would not be a useful marker of neutrophil activation . ^^^ The baseline expression of CD11b and CD 18 on unstimulated neutrophils was similar in heparin and EDTA anti coagulated blood but the response to activation with fMLP was significantly less for the EDTA anti coagulated samples ( p < 0 . 01 in paired t test ) . ^^^ Labelling of CD11b and CD 18 was greater for preparations of isolated neutrophils than for neutrophils in whole blood , and the response to fMLP stimulation tended to be lower for the isolated cells . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Cytoplasmic tails of human complement receptor type 3 ( CR 3 , CD11b / CD 18 ) regulate ligand avidity and the internalization of occupied receptors . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Analysis of blood neutrophils obtained 4 hours after reperfusion has indicated up regulation of CD11b and CD 18 but not CD11a . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Incubation of neutrophils with aspirin increased surface expression of CD11b and CD 18 on neutrophils . ^^^ The aspirin induced increase in PMN adherence to HUVEC was significantly reduced by monoclonal antibodies against CD 18 , CD11b , CD11a , and intercellular adhesion molecule 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The leukocyte surface antigens CD11b and CD 18 mediate the oxidative burst activation of human peritoneal macrophages induced by type 1 fimbriated Escherichia coli . ^^^ Analysis by immunofluorescence activated cell sorting of human peritoneal macrophages from patients undergoing intermittent peritoneal dialysis revealed that they express the CD11 / CD18 surface antigens , with CD11b and CD 18 as the predominant ones . ^^^ Sodium dodecyl sulfate polyacrylamide gel electrophoresis of immunoprecipitates obtained from lysates of 125I labeled macrophages with rabbit polyclonal antibodies against the CD11a c / CD18 complex or against CD 18 , revealed four radioactive bands corresponding to CD11a , CD11b , CD11c , and CD 18 . ^^^ Monoclonal antibodies against CD11b and CD 18 inhibited by 80 and 90 % , respectively , the oxidative burst activation of the macrophages by type 1 fimbriated Escherichia coli , whereas monoclonal antibodies against CD11a , CD11c , and CD 43 were without effect . ^^^ Our results suggest that CD11b and CD 18 ( receptors for C3bi ) serve also as receptors for mannose specific E . coli on human peritoneal macrophages and may be involved in the lectinophagocytosis of the bacteria by these cells . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Human lymphocyte function associated antigen 1 ( LFA 1 , CD11a / CD18 , alpha L / beta 2 ) is a cell surface heterodimer , which , together with Mac 1 ( CD11b / CD18 , alpha M / beta 2 ) and p 150 , 95 ( CD11c / CD18 , alpha X / beta 2 ) , constitutes the leukocyte integrin beta 2 ( CD 18 ) subfamily . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies ( mAbs ) to the beta 2 chain ( CD 18 ) and to the alpha chain of LFA 1 ( CD11a ) reduced the number of IFN alpha producing cells ( IPCs ) by 30 50 % , but mAbs to CD11b or c caused no inhibition . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In this study , the effects of CPB on the expression of neutrophil CD11b and CD 18 ( the components of the Mac 1 adhesion molecule ) were examined ; the effects of membrane versus bubble oxygenators on the expression of neutrophil CD11b and CD 18 were compared ; and the plasma levels of the intercellular adhesion molecule 1 ( cICAM 1 ) , an inducible endothelial adhesion molecule , were measured . ^^^ Blood samples were drawn before , during , and after CPB for determination of the expression of neutrophil CD11b and CD 18 ( immunofluorescent flow cytometry ) , and the plasma cICAM 1 , elastase , lactoferrin ( enzyme linked immunoabsorbent assay ) , and plasma C3a ( radioimmunoassay ) levels . ^^^ CPB caused an immediate and sustained increase in the neutrophil CD11b and CD 18 expression in both groups ; after 60 minutes of CPB , CD11b expression had increased by 116 . 9 % + / 19 . 1 % in group B and by 79 . 3 % + / 8 . 5 % in group M ( p = 0 . 78 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Leukocyte endothelial cell adhesion and shear rate were monitored in rat mesenteric venules during superfusion with either LTB 4 or PAF in the presence or absence of monoclonal antibodies ( MAbs ) directed against either leukocyte ( CD 18 , CD11b ) or endothelial cell [ intercellular adhesion molecule 1 ( ICAM 1 ) , E selectin , P selectin ] adhesion glycoproteins . ^^^ The LTB 4 and PAF induced leukocyte adherence and emigration were significantly attenuated by pretreatment with MAbs directed against CD 18 , CD11b , ICAM 1 , and E selectin , but not P selectin . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
With regard to the integrin family , monocytes expressed beta 1 ( CD 29 ) , alpha 4 , alpha 5 , alpha 6 , beta 2 ( CD 18 ) , CD11a , CD11b , and CD11c subunits , but not alpha 5 ( CD 51 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Furthermore , IL 7 , in a concentration dependent manner , increased the proliferation of mononuclear cells expressing the Mac 1 Ag ( Mac 1+ mononuclear cells ( MNC ) ; CD11b ) up to fivefold in response to CSF 1 , granulocyte macrophage CSF ( GM CSF ) , or IL 3 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
However , in complete intestinal ischemia reperfusion , prevention of PMN adhesion by monoclonal antibodies to CD11b and CD 18 reduces remote but not local intestinal injury . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Except for CD11b , all other leukocyte integrins examined ( CD11a , CD11c and CD 18 ) and their ligand ( CD 54 ) were highly expressed in non CLL cases . ^^^ Adhesion activity of leukemic cells to HUVECs after co culture with HUVECs was well correlated with the expression of CD11b , CD 18 and CD 54 , but showed no predilection for any leukemia subtype . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Expression of LeuCAM on freshly isolated cells was studied using a sensitive peroxidase antiperoxidase method with monoclonal antibodies ( mAB ) against CD11a , CD11b , CD11c , and CD 18 . ^^^ Compared with nonsmoker AM , significantly more AM from smokers expressed CD11b ( p < 0 . 001 ) , CD11c ( p < 0 . 001 ) , CD 18 ( p < 0 . 001 ) , and CD11a ( p < 0 . 004 ) , whereas there was no difference in the expression of other common epitopes of human macrophages such as CD 68 , CD 71 , CD 45 , HLA DQ , and HLA DR . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Under the conditions , cells spontaneously differentiated gradually with days of culture , as assessed by expression of macrophage differentiation antigens such as Mac 1 ( CD11b ) and F4 / 80 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Cells exposed to phorbol myristate acetate ( PMA ) had increased expression of CD11a , CD11b , CD 18 , CD45RO , and HLA DR , whereas expression of CD 15 and CD 30 was markedly decreased . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The effect of in vivo treatment with anti CD11b ( MAC 1 ) antibody ( Ab ) was examined in an inflammatory disease model , the viable moth eaten ( mev ) mutant mouse . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
However , no induction or enhancement of the cell surface expression was observed with respect to CD11b , CD 18 , HLA A , B , C , HLA DR , DQ , DP or CD 57 . ^^^ This phagocytosis was inhibited by the anti CD11b monoclonal antibodies , but not by the anti CD 14 , anti CD 16 , anti CD 18 , anti CD 23 , anti HLA A , B , C or anti HLA DR monoclonal antibodies . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Murine monoclonal anti human antibodies directed against neutrophil adhesion protein receptors CD 35 , CD 18 , CD11b , CD11c and the Fc receptors CD 64 ( Fc gamma RI ) , CD 32 ( FC gamma RII ) and CD 16 ( Fc gamma RIII ) were evaluated regarding their ability to bind to the canine homologues . ^^^ The antibodies against CD 35 , CD 18 , CD11b , CD11c and CD 16 could be used to evaluate the expression of canine homologues . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Expression of adhesion molecules , CD11a , CD11b and CD 18 , and of the function associated molecules CD 3 , CD 4 , CD 8 , CD 16 , CD 19 , CD 56 and CD 57 was assayed on peripheral blood leukocytes from normal control subjects ( n = 10 ) , and from patients with adult periodontitis PD ( n = 9 ) , ankylosing spondylitis AS ( n = 11 ) and rheumatoid arthritis RA ( n = 14 ) . ^^^ On lymphocytes the mean fluorescence intensity ( MFI ) of both CD11b and CD 18 was decreased whereas that of CD 57 was increased . ^^^ On lymphocytes the MFIs of CD11b and CD 18 were decreased , whilst that of CD 16 was increased . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Sufficient protein was purified to allow the first measurement of the 50 % inhibitory concentration ( IC 50 ) for CD11b / CD18 and for the direct comparison of the inhibitory activity of recombinant soluble Mac 1 with that of various CD 18 and CD11b specific monoclonal antibodies . ^^^ Biosynthesis and function of membrane bound and secreted forms of recombinant CD11b / CD18 ( Mac 1 ) . ^^^ Full length ( membrane bound ) and truncated ( secreted ) forms of the beta 2 integrin heterodimer , CD11b / CD18 ( Mac 1 ) , were expressed in a human kidney cell line ( 293 ) that normally does not express leukocyte adhesion molecules ( Leu CAMs ) . ^^^ The biosynthesis of recombinant Mac 1 in 293 cells differed from that reported for leukocytes in that heterodimer formation was not required for CD11b to be exported to the cell surface . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Functional inactivation of neutrophils with a Mac 1 ( CD11b / CD18 ) monoclonal antibody protects against ischemia reperfusion injury in rat liver . ^^^ The role of neutrophil CD11b / CD18 ( Mac 1 ) adhesion proteins in the pathogenesis of hepatic reperfusion injury was investigated in an experimental model . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Transfected leukocyte integrin CD11b / CD18 ( Mac 1 ) mediates phorbol ester activated , homotypic cell : cell adherence in the K 562 cell line . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
While surface density of CD 56 and CD 16 antigens was lower , that of beta 2 integrins ( CD 18 , CD11a , CD11b ) was higher on A NK than on NA NK cells . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Treatment of monocytes with MAC or MAC derived lipid resulted in significant decreases in CD11b , a member of the LFA 1 and Leu M 3 ( CD 14 ) molecule . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We have purified to homogeneity from this conditioned medium a 36 kDa protein , as measured by sodium dodecyl sulfate polyacrylamide gel electrophoresis , which inhibits [ 3H ] thymidine incorporation into the DNA and induces cell surface expression of CD11b , the alpha chain of the adhesion receptor MAC 1 , on the human promyelocytic leukemic cell line HL 60 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
A cytofluorimetric study using integrin monoclonal antibodies ( MAbs ) against CD 18 and CD11b did not reveal any increase in expression of the integrins on the surface of LDL activated monocytes . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
To confirm that these reactions were due to anti Mart , monoclonal antibodies to CD11b and CD 18 were also tested and found to cause cytoplasmic immunofluorescence . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies against the selectins , CD 18 , CD11a , CD11b , and ICAM 1 have all been shown to significantly reduce the influx of neutrophils into sites of inflammation in various animal models . . ^^^ Once the marginated neutrophil forms a stationary adhesion with endothelial cells , it is stimulated by chemotactic factors to downregulate the selectin based adhesion and upregulate adherence dependent on beta 2 integrins , principally CD11a / CD18 ( LFA 1 ) and CD11b / CD18 ( Mac 1 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Moreover , injection of anti complement receptor type 3 ( Mac 1 , CD11b ) resulted in inhibition of anti CD 3 MAb induced antilisterial resistance . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Group M1 / 70 ( n = 6 ) received at reperfusion 15 micrograms / mL of a monoclonal antibody F ( ab ' ) 2 fragment to CD11b , the alpha subunit of the leukocyte adhesion molecule Mac 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Only the combination of mAbs directed to the beta 2 integrin , CD11b ( D12 / SHCL 3 ) , or CD 18 ( MHM 23 ) subunits maximally inhibited ASA and C mediated sperm adhesion to neutrophils ( by 70 % ) or sperm phagocytosis ( by 75 % ) , as well as neutrophil aggregation ( by 96 % ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Flow cytometric comparisons of labeled and unlabeled cells demonstrated that fluorescence labeling of neutrophils and eosinophils with CFDA did not alter basal surface expression of the beta 2 integrins ( i . e . , CD11a CD11b or , CD 18 ) . ^^^ Stimulation of neutrophils with fMLP and eosinophils with PMA resulted in increased surface expression of CD11b and CD 18 which was not altered by CFDA labeling . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
This study showed that the alpha M beta 2 integrin , CR 3 ( Mac 1 , CD11b / CD18 ) served as the beta glucan receptor through one or more lectin sites located outside of the CD11b 1 domain that contains the binding sites for iC3b , ICAM 1 , and fibrinogen . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
CD11a , CD11b , CD 18 , and CD 35 was studied by flow cytometry on neutrophils and monocytes . ^^^ Adherence of human monocytes to haemodialysis membranes : LFA 1 ( CD11a / CD18 ) CR 1 ( CD 35 ) and CR 3 ( CD11b / CD18 ) triggering promotes the biosynthesis of platelet activating factor and adherence . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Reduced expression of membrane proteins was not confined to those anchored through GPI several transmembrane molecules including CD11a CD11b and CD 18 were also reduced on apoptotic PMN , whilst other were little changed ( CD 35 , CD 46 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
L3T4 ( CD 4 ) , Lyt 2 ( CD 8 ) and Mac 1 ( CD11b ) phenotypic leukocytes in murine cryptococcal meningoencephalitis . ^^^ An immunohistological study of L3T4 ( CD 4 ) + and LYT 2 ( CD 8 ) + lymphocytes , Mac 1 ( CD11b ) + monocytes and granulocytes in experimental murine cryptococcal meningoencephalitis was conducted . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Furthermore , a significant decrease was observed in the expression of both structural and functional epitopes of CD11b ( p < 0 . 05 ) and CD 18 ( p < 0 . 05 ) on SF neutrophils at both 4 and 24 h post MP . ^^^ Peripheral blood neutrophils showed decreased expression of CD11b ( p = 0 . 1 ) , CD 18 ( p = 0 . 07 ) , and L selectin ( p = 0 . 09 ) , which approached statistical significance without any associated effects on CD11a , CD15s , or CD 44 . ^^^ CONCLUSION : MP is associated with a marked decrease in CD11b and CD 18 expression on SF neutrophils and , to a lesser extent , peripheral blood neutrophils . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The expression of molecules of the beta 2 integrin family ( CD11a , CD11b , CD11c , and CD 18 ) was explored on 2 human megakaryocytic cell lines and on platelets from different donors by immunofluorescence and flow cytometry using a large panel of mAb . ^^^ CD11a , CD11b , CD11c and CD 18 were detected on the megakaryocytic cell lines DAMI and HEL . ^^^ A low and variable expression of CD11a , CD11b and CD 18 determinants was also detected on resting platelets ; this expression was markedly increased when platelets were activated by thrombin . ^^^ Expression of CD 18 was closely correlated to that of CD11a or CD11b when comparing the fluorescence intensity observed in different experiments . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
METHODS : Cultured murine ISMCs were exposed to IFN gamma for 72 hours and analyzed for Mac 1 ( CD11B CD 18 ) antigen , MHC 2 , and ICAM 1 expression using enzyme linked immunosorbent assay and fluorescence activated cell sorter scan . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Total surface expression of the inducible fibrinogen receptor ( CD 41 ) and surface expression of the activated fibrinogen receptor ( LIBS 1 ) on platelets as well as Mac 1 ( CD11b ) and L selectin ( CD62L ) surface expression on neutrophils were assessed by flow cytometry . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Lidocaine reduces PMN adhesion to endothelial surfaces in vivo , and inhibits upregulation of PMN CD11b / CD18 ( Mac 1 ) in vitro . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Whereas CD11a was increased only on monocytes , CD11b , CD11c , and CD 18 were strongly enhanced on monocytes and polymorphonuclear cells ( PMNs ) after treatment with MDPs . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Most TDC were LFA 1 ( CD11a ) + , CD18+ , ICAM 1 ( CD 54 ) + and CD 53 ( OX 44 ) + , but only certain subsets expressed CD11b and thymocyte markers Thy 1 , CD 2 , CD 4 and CD 8 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
At the eighth week , the number of infiltrating cells reached a peak and consisted of T lymphocytes ( CD 4 , CD 8 ) and macrophages ( ED 1 , MHC class 2 , CD11b , and CD 18 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We examined the expression of adhesion molecules of the integrin family ( CDw49b , CDw49d , CDw49e , CDw49f , CD 18 , CD11a CD11c , and CD11b ) , the immunoglobulin superfamily ( CD 54 , CD 56 , CD 58 , and CD 31 ) , the selectin family ( ELAM 1 , LECAM 1 , and CD 62 ) , and CD 44 , CD41b , and CD42b on platelets , megakaryocytes , and megakaryocytes with CP . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In a retrospective study based on 107 B CLL patients , the expression of the adhesion molecules CD 44 , CD11a , CD11b , CD11c , CD 18 , CD 25 and CD 54 was analysed in bone marrow cryostat sections by immunohistochemistry . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
At the low cation concentration , stopping was largely inhibited by antibodies to the neutrophil integrins CD 18 or CD11b , but not CD11a . ^^^ When neutrophils were pretreated with antibodies to CD11b or CD 18 in 1 mmol / L Ca2+ 0 . 5 mmol / L Mg2+ , stopping was not prevented but delayed . ^^^ Prestimulated neutrophils also bound to platelets and spread , but immobilization was blocked if they were perfused with antibody to CD 18 or CD11b or with low Ca2+ and Mg2+ . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Receptor ligation with either immobilized MoAb to CD 18 or Fas induced membrane disruption and DNA degradation in LAK cells independently of K 562 , and MoAb to CD 18 , CD11a , or CD11b enhanced DNA fragmentation induced by anti Fas . ^^^ In conclusion , ( 1 ) K 562 induced death of LAK cells involves primarily CD 18 , although other molecules , such as Fas , may also be involved ; ( 2 ) K 562 mediated apoptosis of LAK cells requires tyrosine phosphorylation and protease activity ; ( 3 ) engagement of Fas by immobilized MoAb or Fas L on target cells can also kill LAK cells ; and ( 4 ) Fas immobilized MoAb synergizes with coimmobilized MoAb to CD11a , CD11b , or CD 18 for LAK cell killing . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
When the reactivity of mNI 11 and mAbs binding human differentiation antigens such as CD11a , CD11b , CD11c , CD 14 , CD 16 , CD 18 , CD 23 , CD 28 , CD 29 , CD 31 , CD 43 , CD 44 , CD45RA , CD49d , CD 50 , CD 54 , CD 58 , CD 80 , CD 102 , CD 106 , HLA class 1 , or HLA class 2 antigen was compared , no mNI 11 reactivity resembling that of these mAbs was found . mNI 11 markedly induced homotypic cell aggregation of U 937 cells when they were stimulated with LPS . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
To examine the effect of cuprophane hemodialysis on expression of cell surface proteins involved in leukocyte adhesiveness , we monitored CD11b , CD 18 , CD 14 , CD 54 , and plasma soluble CD 54 in 10 patients during hemodialysis with cuprophan dialyzers . ^^^ Leukocyte expression of CD11b and CD 18 increased and CD 14 decreased with hemodialysis , while CD 54 remained unaltered . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies specific for epitopes of the alpha subunit ( CD11b ) and the beta 2 subunit ( CD 18 ) of Mac 1 eliminated lymphocyte adhesion to C . albicans hyphae . ^^^ These results identify Mac 1 ( CD11b / CD18 ) as the surface structure that mediates lymphocyte adhesion to C . albicans . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Infiltrating mononuclear cells expressed CD11a ( 68 % ) , CD 18 ( 73 % ) , CD11b ( 13 % ) , CD11c ( 21 % ) , CD 58 ( 13 % ) , CD 4 ( 44 % ) , CD 8 ( 17 % ) , CD62L ( L selectin ) ( 18 % ) , CD49d ( 38 % ) , CD49e ( 15 % ) , CD 2 ( 56 % ) , and CD 44 ( 77 % ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Immunohistochemical studies of tumor infiltrating lymphocytes in renal cell carcinoma showed a significantly high incidence of CD 3 , CD 8 , CD11b and ICAM 1 in the administration group , while CD 4 , LFA 1 and Ber MAC 3 were increased without significance ( p < 0 . 05 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Incubation of CLC with monoclonal antibodies against HLA DR , CD11b , CD 18 , CD 50 , CD 54 , CD 58 or CD 80 , but not anti major histocompatibility complex class 1 ( MHC 1 ) , inhibited antigen specific T cell proliferation to varying degrees . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
PMN activation was evaluated by study of cell migration , beta 2 integrin expression ( CD11b / CD 18 ) , oxidative burst , and elastase release . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Spherical particles bearing monoclonal antibodies specific for antigens expressed by THP 1 cells ( CD11b , CD 18 , CD 35 , CD 64 ) were then added and adhesion was quantified . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Mechanisms of pertussis toxin induced myelomonocytic cell adhesion : role of Mac 1 ( CD11b / CD18 ) and urokinase receptor ( CD 87 ) . ^^^ Although promoting adhesion to serum or vitronectin coated surfaces , PTX blocked spontaneous cell adhesion to fibrinogen , an endogenous ligand for the Mac 1 receptor ( CD11b / CD18 ) . ^^^ Flow cytometry study showed that expression of the alpha subunit of Mac 1 ( CD11b ) on primed cells was increased by nearly threefold . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Adhesion of neutrophils from patients with BD to HUVEC stimulated with tumour necrosis factor ( TNF ) , interleukin 1 ( IL 1 ) , and lipopolysaccharide ( LPS ) and adhesion molecule ( CD11a , CD11b , CD 18 and L selectin ) expression on the patient ' s neutrophils and lymphocytes were determined , and the serum concentration of IL 8 was measured . ^^^ Monoclonal antibodies to CD11a , CD11b , CD 18 , and ICAM 1 caused varying degrees of inhibition of neutrophil adhesion . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Human PMNs ( 2 . 5 10 10 ( 5 ) ) were saturated with specific monoclonal antibodies for the beta 2 subunits ( CD11a , CD11b , CD 18 ) or nonspecific monoclonal antibodies for 30 minutes on ice before a 1 hour incubation with sICAM 1 ( 75 ng / ml ) at 37 degrees C . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Adhesion of basophils to endothelial cells was inhibited by mAb recognizing CD 18 , CD11a , and / or CD11b , with the pattern and magnitude of inhibition dependent upon the activation state of the basophils and endothelium . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In this study , we examined the preventive effect of monoclonal antibodies ( MAb ) against CD11b ( 1B6 ) , CD11a , or CD 18 ( CL 26 ) on platelet activating factor ( PAF ) induced bowel injury . ^^^ Some rats wee also injected intravenously with 1B6 ( anti CD11b ) , anti CD11a , CL 26 ( anti CD 18 ) , or combined anti CD11a and 1B6 , 30 min before PAF . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
However , blocking of CD11b was ineffective and was not associated with increased protection when combined with anti CD 18 , confirming earlier indications that , under the conditions employed , Mac 1 plays no measurable role in this model of lung injury . ^^^ Dual blockade of CD11b and CD 18 was more effective than blockade of either alone . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Mac 1 ( CD11b / CD18 ) is the predominant beta 2 ( CD 18 ) integrin mediating human neutrophil migration through synovial and dermal fibroblast barriers . ^^^ In contrast , mAb to Mac 1 ( Cd11b ) inhibited ( by 30 40 % ) PMNL migration , and by virtually the same extent as mAb to the beta 1 integrin chain ( CD 18 ) ( 40 % inhibition ) . ^^^ Mac 1 ( CD11b / CD18 ) is the predominant beta 2 ( CD 18 ) integrin mediating human neutrophil migration through synovial and dermal fibroblast barriers . ^^^ Here we investigated the role of the beta 2 integrin family members , lymphocyte function associated ( LFA ) 1 ( alpha L beta 2 or CD11a / CD18 ) and Mac 1 ( alpha M beta 2 or CD11b / CD18 ) , in PMNL migration through HSF and human dermal fibroblast ( HDF ) monolayers . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Despite extensive research in this field , only three members of this integrin subfamily have been described : CD11a / CD18 ( LFA 1 ) , CD11b / CD18 ( Mac 1 ) , and CD11c / CD18 ( p 150 , 95 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In contrast , CD 18 ( beta 2 integrin ) , CD 61 ( beta 3 integrin ) , ICAM 2 , CD11a , CD11b and CD11c were all absent from these cells . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We investigated the expression of adhesion molecules including LFA 1 alpha ( CD11a ) , Mac 1 ( CD11b ) , LFA 1 beta ( CD 18 ) , VLA beta 1 ( CD 29 ) , H CAM ( CD 44 ) , VLA 4 ( CD49d ) , VLA 5 ( CD49e ) , ICAM 1 ( CD 54 ) , N CAM ( CD 56 ) , LFA 3 ( CD 58 ) , VNR beta ( CD 61 ) , and LECAM 1 ( CD62L ) on fresh myeloma cells and human myeloma cell lines . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
These experiments were carried out in the presence of neutralising antibodies to CD 18 , CD11b , LFA 1 , E selectin , P selectin , intracellular adhesion molecule 1 ( ICAM 1 ) , and ICAM 2 ; a novel CD11b / CD18 antagonist , neutrophil inhibitory factor ( rNIF ) ; adenosine receptor agonists ( 5 ' N ethycarboxamido adenosine / N6 cylopentyladenosine ( NECA / CPA ) ) and a platelet activating factor ( PAF ) receptor antagonist lexipafant . ^^^ Recently trypsinised monolayers bound neutrophils in a CD11b / CD18 and CD 18 independent manner . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
These deficits , in part , are due to functional and quantitative defects in neonatal Mac 1 ( CD11b / CD18 ) , whereas LFA 1 ( CD11a / CD18 ) function is similar to that found in adults ( D . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Surface expression of LFA 1 , Mac 1 , p 150 , 95 , and VLA 4 on neutrophils , lymphocytes , and monocytes was measured by fluorescent antibody cell sorting with monoclonal antibodies against CD11a , CD11b , CD11c , CD 18 , CD49d , and CD 29 respectively . ^^^ It was concluded that the integrin adhesion molecules , particularly Mac 1 ( CD11b / CD18 ) , are upregulated on leukocytes in active WG . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Inhibiton of PBL adherence was greatest by CD11b ( 34 % ) and CD11b + CD 18 ( 57 % ) ( P < 0 . 005 , P < 0 . 0025 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We measured the levels of soluble intercellular adhesion molecule 1 ( sICAM 1 ) , CD11a , CD11b , CD 18 , endotoxin , and various inflammatory cytokines to clarify the relationship between adhesive molecules and cytokines in sepsis . ^^^ No significant difference was observed in the CD11a , CD11b , and CD 18 levels between the two groups . ^^^ The sICAM 1 levels significantly correlated with the levels of endotoxin , tumor necrosis factor alpha ( TNF alpha ) , and IL 8 , but CD11a , CD11b , and CD 18 levels did not correlate with endotoxin or cytokine levels . ^^^ These findings suggest that ICAM 1 production is induced by endotoxins and cytokines produced in excess by inflammatory reactions and that endotoxins and cytokines are involved in qualitative , but not quantitative changes in LFA 1 ( CD11a / CD18 ) and Mac 1 ( CD11b / CD18 ) . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Analysis of the effect of IL 5 on surface molecule expression by FACS analysis revealed increased expression of CD11b molecules and decreased expression of L selectin , but no change in the expression of CD11a , CD 18 , CD 29 , CD49d and CD 32 . ^^^ We also show that Mac 1 plays an important role in the regulation of eosinophil activation , since antibodies to CD11b can block IL 5 induced adhesion to IgG and IL 5 induced degranulation . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The primary purpose of the study was to examine circulating neutrophils and monocytes and their plasma membrane expression of CD 64 , CD11b , and CD 18 after two bouts ( B 1 and B 2 ) of eccentric exercise . ^^^ CD 64 , CD11b , and CD 18 expression was determined via direct immunofluorescence and used as an indicator of neutrophil and monocyte activation . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Furthermore , the expression of known markers associated with macrophages , such as c fms , CD11b , CD 18 and granulocyte macrophage colony stimulating factor receptor , were not detected by reverse transcriptase polymerase chain reaction . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
FDCs were immunochemically positive for Mac 1 ( CD11b ) , sialyl Lex ( CD15s ) , CD 22 , integrin beta 1 ( CD 29 ) , CD 40 , very late activation antigen ( VLA ) alpha 3 ( CD49c ) , VLA alpha 5 ( CD49e ) , VLA alpha 6 ( CD49f ) , intercellular adhesion molecule ( ICAM ) 3 ( CD 50 ) , ICAM 1 ( CD 54 ) , B 7 ( CD 80 ) , and vascular cell adhesion molecule ( VCAM ) 1 ( CD 106 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
No significant changes in the cell surface expression of adhesion molecules CD11a , CD11b , CD11c , CD 18 , CD 54 , CD 44 , CD49d ( alpha 4 integrin ) and CD62L ( L selectin ) were observed on MV infected monocytes . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The expression of CD 18 , CD11a , CD11b and CD11c on the surface of polymorphonuclear leukocytes was examined by flow cytometry with monoclonal antibodies . ^^^ Percent change in the expression of CD 18 at 48 h after PTCA ( from baseline ) and that of CD11b were correlated ( r = 0 . 73 , p = 0 . 0008 ) in patients with restenosis . ^^^ OBJECTIVES : This study evaluated the role of neutrophil adhesion molecules LFA 1 ( CD11a / CD18 ) , Mac 1 ( CD11b / CD18 ) and p 150 , 95 ( CD11c / CD18 ) in patients undergoing percutaneous transluminal coronary angioplasty ( PTCA ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Both isomers were also able to partially block the up regulation of complement receptors 1 ( CR 1 ; CD 35 ) and 3 ( CR 3 ; CD11b / CD 18 ) in N formyl methionyl leucyl phenylalanine ( FMLP ) stimulated neutrophils . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
When the reactive patterns between mNI 58A and the mAbs to various human differentiation antigens ( CD11a , CD11b , CD11c , CD 14 , CD 16 , CD 18 , CD 23 , CD 28 , CD 29 , CD 31 , CD 43 , CD 44 , CD45RA , CD 50 , CD 54 , CD 58 , CD 80 , CD 102 , CD 106 , HLA class 1 and class 2 antigen ) were compared , that of mNI 58A was found to be similar to those of the leukocyte function associated antigen 1 ( LFA 1 ) mAbs . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Expression of both CD11b and CD 18 subunits of Mac 1 was lower on CDE which were continuously ( = chronically ) exposed to IL 5 than on CDE which were cultured without IL 5 for the final week of culture . ^^^ CD11b expression on cells grown without IL 5 was 71 . 3 + / 5 . 92 ( mean specific fluorescence value [ MSF ] as measured by flow cytometry ) versus 52 . 5 + / 4 . 48 MSF for Mac 1 alpha ( CD11b ) on CDE grown in the continued presence of 2 10 10 11 mol / L IL 5 ( P < . 01 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Both the early and the late events were abolished in the presence of specific antibodies against CD ( cluster of differentiation ) 11a , CD11b , CD 18 and ICAM ( intercellular adhesion molecule ) 1 , but not CD11c . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Whereas the roles of the common beta chain ( CD 18 ) and of the alpha subunit of MAC 1 ( CD11b ) have been studied extensively , the role of the alpha subunit of LFA 1 ( CD11a ) remains less well defined . ^^^ This phenomenon involves interactions between neutrophil beta 2 integrins ( CD11a / CD18 [ lymphocyte function associated antigen 1 , or LFA 1 ] , CD11b / CD18 [ membrane attack complex 1 , or MAC 1 ] , and CD11c / CD18 [ p 150 , 95 ] ) and their endothelial ligands . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Differential leukocyte count was performed on cytospin preparations and the expression of integrin alpha ( CD11a , CD11b , CD11c ) and beta ( CD 18 ) chains was assessed on granulocytes and mononuclear cells by immunocytology . ^^^ The numbers of neutrophils expressing CD11b and CD 18 , but not CD11c or CD11a , were increased in the subjects with airway obstruction compared with those without airway obstruction . ^^^ CD11b and CD 18 positive neutrophils were negatively correlated with FEV1 / VC ratio ( p < 0 . 01 ) . ^^^ No significant correlations were found between CD11a , CD11b , CD11c , CD 18 positive mononuclear cells and lung function measurements . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Effects of conventional peritoneal dialysates on leukocyte adhesion and CD11b , CD 18 and CD 14 expression . ^^^ Leukocyte CD11b , CD 18 and CD 14 were measured by flow cytometry using monoclonal antibodies in otherwise unstimulated cells or 60 minutes after lipopolysaccharide ( LPS ) stimulation . ^^^ G PDS , as well as the mixtures of spent and fresh G PDS , significantly depressed the basal PMN expression of adhesion receptors CD11b and CD 18 and monocyte expression of CD 14 , and substantially mitigated the LPS mediated up regulation of CD11b and CD 18 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Treatment of Dex induced monocytes with antibodies against RM3 / 1 , CD11a , CD11b , CD11c , CD 14 and CD 18 resulted in suppression of adhesion to stimulated HUVEC by 46 % , 18 % , 17 % , 12 % , 25 % and 15 % , respectively . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Our laboratory measured the upregulation of the neutrophil adhesion molecules CD11b and CD 18 during CPB but did not demonstrate significant differences between membrane and bubble oxygenators . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The surface expression of CD11b and CD 18 was assessed by immunofluorescence , immunobead binding , flow cytometry , and immunoprecipitation with anti CD11b and anti CD 18 monoclonal antibodies ( MAbs ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies ( 2 mg / kg ) to ICAM 1 and the alpha chain ( CD11a , CD11b ) or the beta chain ( CD 18 ) of beta 2 integrins had no significant effect on hepatic neutrophil count after endotoxin . ^^^ In contrast , these antibodies inhibited peritoneal neutrophil infiltration in response to glycogen administration by 28 % ( CD11b ) , 60 % ( CD11a , ICAM 1 ) , and 92 % ( CD 18 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The expression of surface adhesion molecules on granulocytes , monocytes ( CD11a , CD11b , CD11c , CD 18 , L selectin ) , and platelets ( P selectin , gpIIb IIIa ) was determined after incubation with different graft surfaces [ expanded polytetrafluoroethylene ( ePTFE ) or woven Dacron ] . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Examination of the spleen cell population by FACS analysis revealed an increase in the percentage of monocytes as defined by expression of CD11b , the Mac 1 antigen . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Time course of beta 2 integrin CD11b / CD18 ( Mac 1 , alpha M beta 2 ) upregulation on neutrophils and monocytes after coronary artery bypass grafting . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We investigated the roles of CD11a , CD11b , CD 18 , and CD 54 adhesion molecules in non adherent peripheral blood mononuclear cells ( NPBMC ) induced DNA fragmentation of cytomegalovirus ( CMV ) infected cells . ^^^ Treatment of NPBMC with monoclonal antibodies to CD11a , CD11b , CD 18 , and CD 54 significantly reduced cytotoxicity against CMV infected cells . ^^^ A combination of anti CD11a , anti CD11b , and anti CD 18 antibodies further inhibited cytotoxicity against CMV infected cells . ^^^ These results indicate that LFA 1 ( CD11a / CD18 ) , Mac 1 ( CD11b / CD18 ) , and ICAM 1 ( CD 54 ) adhesion molecules are involved in natural killer ( NK ) cell induced DNA fragmentation in CMV infected cells . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
This is supported by our in vitro studies , in which phagocytosis of opsonized particles by human neutrophils rapidly induced apoptosis that could be blocked with CD11b / CD 18 antibodies . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Finally , crosslinking of CD11a or CD11b , but not of CD 18 , with mAbs and F ( ab ' ) 2 fragments produced a delay in spontaneous apoptosis , whereas crosslinking of L selectin with mAb or its natural ligand , sulfatides , accelerated the apoptotic process . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Moreover , CD11a and CD 18 were among two adhesion molecules which showed a sharp increase in expression , whereas no changes on expression of CD11b , CD 44 and CD 54 were detected . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Increased surface expression of CD 18 and CD11b in leukocytes after tourniquet ischemia during elective hand surgery . ^^^ Comparable expression of CD 18 , CD11a , CD11b , and CD11c could be detected by immunofluorescence in leukocytes from samples drawn from either arm before tourniquet application . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Neutrophil activation was examined in 22 women with pre eclampsia and 22 age and gestation matched control subjects using whole blood flow cytometry to assess basal and platelet activating factor stimulated CD11b and CD 18 . 2 . ^^^ There were no between group differences in expression of either CD11b or CD 18 at either 6 weeks or 6 months post partum , either before or after platelet activating factor stimulation . 5 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The cell surface markers included complement receptors [ CR 1 ( CD 35 ) , CR 2 ( CD 21 ) , CR 3 ( CD11b , CD 18 ) ] , immunoglobulin Fc receptors [ FcgammaRII ( CD 32 ) , FcgammaRIII ( CD 16 ) ] , proteins important for lymphocyte function [ CD 3 , CD 4 , CD 8 , CD 19 , CD 25 , CD45RA ] , and the framework antigen HLA ABC . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Because neutrophil aggregation induced by phorbol myristate acetate ( PMA ) is accompanied by de novo phosphorylation of the CD 18 cytoplasmic tail , we sought to determine whether CD11b / CD18 phosphorylation is separately regulated in the different cellular compartments . ^^^ In contrast , only marginal phosphorylation of specific granule associated CD 18 or CD11b was observed . ^^^ Addition of intact specific granule membranes to the plasma membranes from PMA treated neutrophils markedly decreased phosphorylation in both CD11b and CD 18 subunits . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Fluorescence microscopy studies revealed a poor correlation between CD11b ( Mac 1 ) positive cells ( TAMs ) and the appearance of doxorubicin fluorescence . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We therefore investigated the effect of sera , positive for anti neutrophil cytoplasmic antibodies ( ANCA ) or anti nuclear antibodies ( ANA ) from patients with vasculitis , on granulocyte expression of the adhesion molecule Mac 1 ( CD11b ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Treatment with retinoic acid ( RA , 1 microM ) or 1 , 250 ( OH ) 2 vitamin D 3 ( 1 , 25 D 3 , 10 nM ) increased the expression of the monocytic surface antigens CD11b plus CD11c , and CD11b , CD11c , CD 14 plus CD 18 , respectively . ^^^ Addition of the cyclooxygenase inhibitors indomethacin ( 10 microM ) or piroxicam ( 20 microM ) slightly increased expression of CD11b and CD 18 in cells differentiated with RA , but did not alter expression of surface antigens in cells treated with 1 , 25 D 3 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
FACS analysis showed that PMN treated with rHDL and subsequently washed were resistant to FMLP induced CD11b / CD 18 up regulation . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Sections from the liver and spleen by immunocytochemistry technique showed the presence of CD11b , c / CD 18 antigens in the control and infected animals and no qualitative or quantitative differences in the liver . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Expression of CD11b , CD 18 , and L selectin was measured on granulocytes and lymphocytes in whole blood within 1 hour of blood collection . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
To better define the specific function of Mac 1 ( CD11b ) versus LFA 1 ( CD11a ) and the other CD 11 integrins in vivo , we have disrupted murine CD11b by targeted homologous recombination in embryonic stem cells and generated mice which are homozygous for a mutation in CD11b . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
When the reactivity of mNKES and mouse mAbs recognizing the human adhesion associated antigen ( CD 10 , CD11a , CD11b , CD11c , CD 14 , CD 16 , CD 18 , CD 23 , CD 28 , CD 29 , CD 31 , CD 43 , CD 44 , CD45RA , CD 50 , CD 54 , CD 58 , CD 80 , CD 102 , CD 106 , and HLA class 1 , and HLA class 2 antigen ) with various cell lines was compared , mNKES reactivity was found to be unique , not resembling that of any of these mouse mAbs . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
However , 7E3 mAb is not selective for platelet IIb / IIIa but also cross reacts with the leukocyte integrin Mac 1 ( alpha M beta 2 , CD11b / CD18 ) and the vitronectin receptor ( alpha 5 beta 3 , CD51 / CD61 ) . ^^^ Flow cytometry demonstrated that 7E3 bound to THP 1 monocytic cells and identified a subpopulation ( approximately 10 % ) of Mac 1 that was qualitatively similar to that recognized by CBRM1 / 5 , a mAb directed to an activation specific neoepitope present on a subset of Mac 1 molecules . mAb 7E3 bound to K 562 cells transfected with just the alpha subunit ( CD11b ) of Mac 1 but not to nontransfected cells , confirming a direct interaction between 7E3 and Mac 1 . mAb 7E3 and c7E3 Fab blocked the adhesion of Mac 1 bearing cells to FGN ( 80 + / 11 % and 78 + / 9 % inhibition , respectively ) and ICAM 1 ( 62 + / 14 % and 62 + / 17 % ) . ^^^ Thus , c7E3 Fab cross reacts with the CD11b subunit of Mac 1 and interrupts cell extracellular matrix and cell cell adhesive interactions and may thereby influence the recruitment of circulating monocytes to sites of vessel injury . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Peripheral blood patient neutrophils exhibited normal expression of CD 14 , but failed to respond to treatment with LPS ( 100 1000 ng / ml for 30 min at 37 degrees C ) , a treatment that caused increased expression of the surface markers , C 10 , CD 18 , CD11b , CD 67 , and CD 45 , and decreased expression of L selectin in normal neutrophils . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The glycoproteins were shown to be : the alpha subunit ( CD11b ) of the CD11b / CD18 integrin ( Mac 1 antigen ) ; the lysosomal membrane glycoproteins LAMPs 1 and 2 which are known in part to be expressed at cell surfaces ; the Mac 3 antigen , a mouse macrophage differentiation antigen defined by the M3 / 84 monoclonal antibody and related immunochemically to LAMP 2 ; the heavy chain of CD 98 , a 125 kDa heterodimeric glycoprotein identified by the 4F2 / RL388 monoclonal antibodies respectively on human and mouse monocytes / macrophages and on activated T cells . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In our earlier studies , we have shown that transcripts for Fc gammaRII and Fc gammaRIII are lowered in CML granulocytes , while no such trend was seen for CD11b , CD 18 and actin , the other molecules involved in this process . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Although L selectin expression on PMN was not changed by either 5 or 3 micron filtration , CD 18 and CD11b were increased by 3 micron filtration ( P < 0 . 05 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
However , 15 min after coronary angioplasty , there was an increase in neutrophil CD 18 and CD11b , monocyte CD 14 and platelet glycoprotein IIb / IIIa expression and a decrease in neutrophil L selectin expression ( 189 + / 25 % , 163 + / 27 % , 158 + / 35 % , 141 + / 22 % and 31 + / 10 % , respectively , p < 0 . 01 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
G CSF ( 3 5 micrograms / kg ) caused an enhanced expression of CD11b , CD 18 , CD 35 , CD 64 ( Fc gamma RI ) and CD 32 ( Fc gamma RII ) , respectively . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Among the beta 2 integrins , the levels of Mac 1 ( CD11b ) and p150 . 25 ( CD11c ) were positive , while LFA 1 ( CD11a ) was not detectable . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The HMC 1 cells clearly expressed CD11a , CD 18 and CD 54 , while expression of CD11b and CD11c was low . ^^^ Phorbol myristate acetate ( PMA ) induced a time and dose dependent up regulation of CD11a , CD11b , CD11c , CD 18 and CD 54 that was inhibited by cycloheximide , suggesting a dependence on de novo protein synthesis . ^^^ Enhanced expression of CD11a , CD11b , CD11c and CD 18 could also be confirmed at the gene level as demonstrated by semi quantitative reverse transcription polymerase chain reaction ( RT PCR ) . ^^^ Only LTB 4 was shown specifically to up regulate CD11a and CD 18 , but not CD11b or CD11c . ^^^ In the present investigation , the authors studied the expression and regulation of the beta 2 integrins LFA 1 ( CD11a / CD18 ) , Mac 1 ( CD11b / CD18 ) , p 150 , 95 ( CD11c / CD18 ) and of the LFA 1 / Mac 1 counter receptor intercellular adhesion molecule 1 ( ICAM 1 ; CD 54 ) on leukaemic ( HMC 1 cell subclone 5C6 ) and on normal mature human skin mast cells . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We found that long term reconstituting stem cells decrease their expression of the growth factor receptor c kit by 10 fold and increase their expression of the integrin Mac 1 ( CD11b ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Inhibition of CD 18 or CD11b attenuates acute lung injury after acid instillation in rabbits . ^^^ Although a prior study demonstrated that acid induced neutrophil influx into the air spaces was not CD 18 dependent , we hypothesized that either a neutralizing anti CD 18 monoclonal antibody ( MHM 23 ) or a neutrophil inhibitory factor ( NIF ) , NIF ( CD11b , 18 ) , might attenuate acid induced lung injury in rabbits by interfering with neutrophil activation . ^^^ In acid instilled rabbits without the anti CD 18 monoclonal antibody or NIF ( CD11b , 18 ) , severe lung injury developed . ^^^ In acid instilled rabbits , pretreatment ( 5 min before acid ) with the anti CD 18 monoclonal antibody ( 2 mg / kg i . v . ) or pretreatment with the NIF ( anti CD11b , 18 , 10 mg / kg i . v . ) prevented 50 70 % of acid induced abnormalities in oxygenation , the increase in extravascular lung water , and extravascular protein accumulation . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
However , adhesion to myeloperoxidase was inhibited by monoclonal antibodies to alpha M ( CD11b ) or to beta 2 ( CD 18 ) integrin subunits , but not by antibodies to alpha L ( CD11a ) , alpha M ( CD11c ) , or to other integrins . ^^^ Myeloperoxidase mediates cell adhesion via the alpha M beta 2 integrin ( Mac 1 , CD11b / CD18 ) . ^^^ It is evident that myeloperoxidase supports cell adhesion , a function which may be of considerable importance for leukocyte migration and infiltration in inflammatory reactions , that alpha M beta 2 integrin ( Mac 1 or CD11b / CD18 ) mediates this adhesion , and that the alphaM beta 2 integrin mediated adhesion to myeloperoxidase is distinct from the previously reported ability of this integrin to bind to certain denatured proteins at high concentrations . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Up regulation of ICAM 1 was also observed following stimulation with other mAb specific for CD11a ( S6F1 ) , CD11b ( JML H 11 ) , and CD 18 ( IB 4 ) ; all of these mAb recognized members of the beta 2 integrin family , but not with isotype matched control mAb . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Granulocyte and monocyte surface adhesion molecule expression was determined indirectly using a panel of monoclonal antibodies against CD11a , CD11b , CD11c , CD 18 , and L selectin in donor white blood cells exposed to patient plasma . ^^^ CD11b , CD11c , and CD 18 molecules on both granulocytes and monocytes were significantly upregulated 60 minutes after the endovascular procedure compared to conventional surgery . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
They expressed low but positive levels of the CD 14 marker , and intermediate levels of both CD11b ( Mac 1 ) and CD 54 ( ICAM 1 ) adhesion molecules . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
CD11b and another triggering molecule for eosinophil degranulation , CD 18 , showed a significantly inhibited expression of PAF stimulation using a combination of 10 ( 4 ) M theophylline and various concentrations of procaterol ( 10 ( 5 ) M 10 ( 7 ) M ) compared with the inhibition of 10 ( 4 ) M theophylline or procaterol ( 10 ( 5 ) M 10 ( 7 ) M ) , but GM CSF stimulated eosinophils were not inhibited . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Fluorescence activated cell scanning ( FACS ) analyses indicated that the vitamin D derivatives readily induced the expression of the monocyte associated cell surface antigen , CD 14 , and also the beta 2 integrins , CD11b and CD 18 in both cell lines after 48 h and 96 h treatment . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
RESULTS : Indomethacin caused a concentration and time dependent upregulation of CD11b and CD 18 expression and PMN adhesion to endothelial cells . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We examined whether interaction of PMNs with E selectin also leads to activation of CD11b / CD18 ( Mac 1 , alphaMbeta 2 ) , an event that can promote firm adhesion . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
BECs cocultured in transwell chambers below confluent monolayers of endothelial cells promoted the transendothelial migration of neutrophils , which was blocked by antibodies to CD 18 or CD11b but only partially inhibited by blocking antibodies to IL 8 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In addition , single cell suspensions of the Lewis lung and B16 / BL6 tumors were prepared and the presence of macrophages was determined with an FITC labeled rat antimouse CD11b ( MAC 1 ) antibody . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Pertussis toxin ( PTX ) was thought to bind Mac 1 integrin receptor ( CD11b / CD18 ) on TGF beta1 / D3 primed U 937 cells and induced cellular adhesion to serum coated plate . ^^^ In addition , PTX induced myelomonocytic cell adhesion was abolished in the presence of genistein ( 100 microM ) , a specific tyrosine kinase inhibitor . 2LPM19c ( 2 microg / ml ) , a mouse monoclonal antibody against the CD11b subunit of Mac 1 integrin , or ethylenediamine tetraacetic acid ( EDTA , 5 mM ) prevented PTX mediated U 937 cell adhesion . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Expression of adhesion molecules CD11b and CD 18 , and phagocytosis was significantly ( P < 0 . 05 ) better in the dogs with pyoderma compared with the healthy dogs . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Both pretreatments with G CSF increased LPS induced peripheral neutrophilia ; the expression of CD11b , CD 18 , and CD 67 ; and the release of elastase and lactoferrin . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Surface expression of integrins was measured by FACS analysis in 19 healthy subjects ( 16 males , 3 females , 36 . 6 + / 8 . 7 years , 177 . 1 + / 7 . 5 cm , 70 . 3 + / 8 . 1 kg ) before and after submaximal exercise ( 3 h run ) using monoclonal antibodies against cd11a , cd11b , cd11c , cd 18 , cd 29 and cd 49 . ^^^ Since leucocyte adhesion is mediated by integrins , we tested the hypothesis that surface expression of the integrin adhesive receptors LFA 1 ( cd11a / cd18 ) , MAC 1 ( cd11b / cd18 ) , gp 150 / 95 ( cd11c / cd18 ) , and VLA 4 ( cd29 / cd49 ) is decreased by moderate endurance exercise . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We assessed the role of 5 lipoxygenase activity in hemodialysis induced upregulation of the granulocyte adhesion molecules , CD11b and CD 18 , and granulocyte adhesion to human umbilical vein endothelial cells in an ex vivo dialysis model . 5 Lipoxygenase was effectively inhibited by preincubation of human whole blood with the specific inhibitor , BAY X 1005 . ^^^ Dialysis with new cuprophane but not with new acrylonitrile membranes significantly upregulated expression of CD11b and CD 18 by 6 fold and 4 fold , respectively . ^^^ Inhibition of 5 lipoxygenase did not affect the expression of CD11b and CD 18 during dialysis with either of the two membranes . ^^^ In contrast to the enhanced expression of CD11b and CD 18 , adhesion of granulocytes to human umbilical vein endothelial cells did not increase during dialysis , nor was it affected by BAY X 1005 . ^^^ These data indicate that ex vivo dialysis with cuprophane membranes upregulates expression of CD11b and CD 18 on granulocytes independent of the activation of 5 lipoxygenase . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Concomitant alteration of CD11b , CD 18 , and CD49d ( VLA alpha 4 ) cell adhesion molecule expression was not seen with either MIP 1 alpha type . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
A panel of monoclonal antibodies against CD11a , CD11b , CD11c , CD 18 and L selectin was used . ^^^ RESULTS : The expression of CD11a adhesion molecules only showed slight variations regarding granulocytes , but was more pronounced on monocytes , however , without significant differences between the two patient groups , CD11b , CD11c and CD 18 molecules on both granulocytes and monocytes were significantly upregulated 60 min after the endovascular procedure compared to conventional aneurysm repair , and L selectin molecules were by this time correspondingly cleaved off . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The inhibitory action of PU . 1 on CD11c is in contrast with its positive regulatory effect on the CD11b and CD 18 integrin gene promoters , which might contribute to the differentially regulated expression of CD11b / CD18 and CD11c / CD18 during monocyte extravasation and terminal maturation . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Whole blood samples were immunolabelled and flow cytometry used to measure the distribution and expression of the adhesion molecules CD11b , CD 18 , CD62L on neutrophils , monocytes and lymphocytes , in addition to CD 64 on neutrophils and monocytes , and CD 14 on monocytes . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Beta Glucan laminarin produced background inhibition . mAbs against CD 14 , CD11b , and CD 18 did not prevent FITC MP 2 binding to PBMCs , implying that these receptors are not involved in MP 2 recognition by PBMCs . mAb against CD 14 blocked ( > 90 % ) MP 2 induced TNF alpha release by PBMCs , while mAbs against CD11b / CD18 caused no inhibition . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Using mAbs directed against human CD 14 and components of the human LFA 1 complex , we identified coelomocyte surface antigens cross reactive with CD 14 , CD11b and CD11c . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
On alveolar macrophages obtained after the release of aortic cross clamp , macrophages as compared with alveolar macrophages obtained before cardiopulmonary bypass , there was a significant enhancement of CD11a , CD11b , CD11c , and CD 18 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The panel included probes specific for the CD 45 , CD 34 , P glycoprotein ( mdr 1 ) , Ly 6A / E ( Sca 1 ) , heat stable antigen ( CD 24 ) , Fc receptor for IgG FcgammaRII ( CD 32 ) , CD 44 , CD 22 , and ICAM 1 ( CD 54 ) genes , as well as alphaL ( CD11a ) , alphaM ( CD11b ) , beta 2 ( CD 18 ) , alpha 4 ( CD49d ) , alpha 5 ( CD49e ) , beta 1 ( CD 29 ) , and beta 7 integrin subunit sequences . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
VCAM 1 and ICAM 1 were significantly ( P < 0 . 05 ) increased after 4 d of G CSF administration as was neutrophil cell surface expression of CD11b and CD 18 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The effects of linoleic acid , linoleic acid anilide , and arachidonic acid on the expression of CD11b / CD 18 , CD11c / CD18 integrins and L selectin on human neutrophils were studied by flow cytometry in a whole blood assay . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
As an additional index of leukaemic cell differentiation , induction of expression of the phenotypic cell surface antigen , CD 14 , and the beta 2 integrins , CD11b and CD 18 by the vitamin D and retinoid compounds were monitored using fluorescence activated cell sorting ( FACS ) analyses . ^^^ CD11b and CD 18 antigen expression were also raised above control levels . ^^^ However , CD11b and CD 18 were both readily increased in U 937 and HL 60 cell cultures . ^^^ CD11b and CD 18 antigen expression was also enhanced in both cell lines with cotreatment . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The CD8+CD45RA+CD27 population contains T cells expressing high levels of CD11a , CD11b , CD 18 , and CD49d , whereas CD62L ( L selectin ) is not expressed . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Expressions of CD11b , CD 18 and CD 54 , but not CD11a or CD11c , were higher on monocytes freshly isolated by density gradient separation and plastic adherence as compared with cells labelled directly in whole blood . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In addition , the number of conjugates and the expression of some adhesion molecules ( CD11a , CD11b , CD 18 , CD 2 , and CD62L ) were not modified by ox LDL . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Monocyte expression of Fc gammaRI , Fc gammaRII , Fc gammaRIII , CD11a , CD11b , CD 18 , and HLA DR also significantly increased with peak expression at 48 hours . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The effects of rhG CSF administration on fMLP induced neutrophil CD11b and CD 18 upregulation were studied in nine patients suffering from intermediate and high grade non Hodgkin ' s lymphomas . ^^^ Five patients showed an impaired baseline CD11b and CD 18 upregulation , which was corrected by rhG CSF therapy . ^^^ Four patients showed a normal baseline CD11b and CD 18 upregulation , but this function was reduced by rhG CSF therapy . ^^^ We conclude that , while in some patients rhg CSF is able to improve neutrophil CD11b and CD 18 upregulation in response to chemotactic agents , in other patients a decrease of this function can occur , maybe due to a relative immaturity of the circulating neutrophils induced by rhG CSF . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Blood of healthy volunteers was tested for several phagocytic functions in the presence of MoAbs against surface ( CD 16 , CD11b , CD 18 , NB 1 ) and cytoplasmic ( proteinase 3 ; PR 3 ) molecules . ^^^ Phagocytic activity was inhibited by anti CD11b and / or anti CD 18 MoAbs . ^^^ Zymosan induced chemiluminescence was reduced by MoAbs anti CD 16 , CD 18 , and NB 1 , enhanced by anti PR 3 MoAb , and less enhanced by anti CD11b . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The role of coagulation factor 10 as a ligand for CD11b / CD18 ( Mac 1 , alphaMbeta 2 ) in leukocyte adhesion was investigated . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We have studied the post injury expression of the lymphocyte , monocyte and neutrophil adhesion molecules CD11a ( LFA 1 ) , CD11b , CD11c , CD 29 ( beta 1 integrin ) and CD62L ( L selectin ) in a group of 36 trauma patients , 13 of whom had suffered major trauma ( ISS > or = 16 ) , 15 moderate trauma ( ISS = 9 15 ) and eight minor trauma ( ISS < 9 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The aim of the present investigation was to study the distribution of CD11b , CD 18 , and ICAM 1 over the various cell populations present in the margin zone of the actively spreading psoriatic lesion and distant uninvolved psoriatic skin . ^^^ Polymorphonuclear leukocytes appeared to invade the skin well after the appearance of CD11b and CD 18 positive cells . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In the remaining 12 patients who developed restenosis , the MFI values for CD 18 and CD11b were increased at 24 h and 48 h after the PTCA . ^^^ To investigate the neutrophil activation process following percutaneous transluminal coronary angioplasty ( PTCA ) , we examined the expressions of Mac 1 ( CD11b / CD18 ) , L selectin ( CD62L ) , and sialyl LewisX ( SLX ) on the surface of neutrophils after the PTCA procedure , by flow cytometric analysis . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
CD11b , CD 18 , and CD 32 monoclonal antibodies inhibited degranulation , demonstrating dependence on complement receptor type 3 ( CR 3 ) , and the low affinity Fc receptor for IgG . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Previous studies have shown that triggering multiple myeloma ( MM ) cells via CD 40 induces IL 6 mediated autocrine growth as well as increased expression of cell surface adhesion molecules including CD11a , CD11b , CD11c , and CD 18 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Neutrophil migration from the bloodstream involves alterations in surface expression of the adhesion molecules L selectin and Mac 1 ( CD11b / CD18 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Expression of CD11a , CD11b and CD 18 on neutrophils was assessed by immunofluorescence flow cytometry 30 min after the stimulation of neutrophils with 10 ( 7 ) M platelet activating factor ( PAF ) . ^^^ PAF induced upregulation of CD11b and CD 18 on neutrophils and IL 1 beta increased surface expression of ICAM 1 and VCAM 1 on HUVEC . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Serial sections of tissue were stained immunohistochemically with the primary antibodies CD11b , mac 1 , anti PDGF , and alpha smooth muscle actin . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In the presence of FMLP , adhesion induced by both media was significantly greater than by FMLP alone ( P < . 001 ) and was significantly blocked ( P < . 01 ) by antibodies to CD11b and CD 18 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We used U 937 derived clones overexpressing either antisense RNAs antisense to CD11b and CD 18 mRNAs or mRNA from a truncated mutant CD11b gene . ^^^ Here we have shown that the plastic adherence of phorbol ester treated U 937 cells is mediated by expression of integrin Mac 1 ( CD11b / CD18 ) on the cell surface and that these adherent cells exhibit anoikis ( apoptosis when adherent cells are detached or adherence is inhibited ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
HOCl production was not significantly attenuated when neutrophils were activated with SAIgG in the presence of surface adherent C1q or when CR 3 was ligated by F ( ab ' ) 2 derived from mAb having specificity for the CD11b ( mAb M1 / 70 ) or CD 18 ( mAb TS 1 ) subunits of CR 3 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
By using flow cytometric analysis of cells in whole blood expressing high levels of CD 14 , we found a subpopulation of monocytes ( 8 % of total ) with higher scatter parameters , high capacity to produce reactive oxygen species ( ROS ) , stronger expression of Lewis 10 ( CD 15 ) , sialyl Lewis 10 , CD11b and CD 18 antigens , as well as an increased polymerized actin content . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The expression of CAM was analyzed by flow cytometry using monoclonal antibodies against CD49d , CD 29 , CD11a , CD11b , CD11c , CD 18 , CD62L , CD 54 and CD 44 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
PMN adhesion to factor H in the presence or absence of C5a was blocked specifically by mAbs against CD11b or CD 18 . ^^^ The adhesion of PMNs to glycosaminoglycan factor H was blocked by mAbs to CD11b and CD 18 . ^^^ Affinity purification using factor H Sepharose followed by immunoprecipitation using mAbs to various integrin chains identified Mac 1 ( CD11b / CD18 ) as a factor H binding receptor . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
MATERIAL AND METHODS : Using immunofluorescence phenotyping and flow cytometry , the expression of leukocyte adhesion molecules CD 54 , CD11a , CD11b and CD 18 in peripheral blood were measured within 12 h after onset of ischemia in 20 patients with stroke . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
This study examined interaction of the CD11a , CD11b , and CD 18 subunits of the beta 2 integrin with ICAM 1 , transfected into Chinese hamster ovarian ( CHO ) cells to avoid effects of other adhesion molecules . ^^^ Blockade with a monoclonal antibody ( MAb ) to ICAM 1 or MAbs to CD11a , CD11b , or CD 18 reduced PMN mediated cytotoxicity to baseline . ^^^ To examine the role of adhesion in cytotoxicity , we studied beta 2 integrin mediated PMN adhesion to ICAM 1 transfected CHO cells and found that MAbs for CD11a , CD11b , and CD 18 all abrogated PMN cytotoxicity despite disparate effects on adhesion . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The increased adherence of activated heterophils can be partially blocked by either anti alpha M ( CD11b ) or anti beta 2 ( CD 18 ) antibodies in a dose dependent manner . ^^^ However , antibodies to the Mac 1 complex ( CD11b / CD18 ) block only the adherence of SE ILK stimulated heterophils . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The cell surface expression of the integrin proteins CD11a , CD11b , CD 18 and CD 29 on neutrophils was assayed by means of flow cytometry . ^^^ No differences were seen between patients and controls in cell surface expression of the integrin proteins CD11a , CD11b , CD 18 and CD 29 on neutrophils , nor was there any difference in these parameters between patients with and without extra glandular symptoms . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Monocyte and U 937 lymphocyte function associated antigen 1 ( CD11a / CD18 ) and macrophage antigen 1 ( CD11b / CD 18 , alpham / beta2 ) were both upregulated upon MP stimulation , but an increase in p 150 , 95 ( CD11c / CD18 ) , very late antigen 1 , or ICAM 1 expression was not observed . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The expression of CD 18 and CD11b on the surface of neutrophils was determined by flow cytometric analysis . ^^^ The expression of both the CD 18 and CD11b in the coronary sinus blood gradually increased and reached its maximum at 48 hours after angioplasty . ^^^ Percentage increases of CD 18 and CD11b expression and the increase of the sICAM 1 levels at 48 hours after angioplasty ( as ratios to baseline values before angioplasty ) were less in the Cutting Balloon angioplasty group than in the conventional balloon angioplasty group ( CD 18 , 1 . 10+ / 0 . 05 versus 1 . 31+ / 0 . 05 , P < 0 . 05 ; CD11b , 1 . 23+ / 0 . 06 versus 1 . 72+ / 0 . 10 , P < 0 . 001 ; sICAM 1 , 1 . 12+ / 0 . 05 versus 1 . 25+ / 0 . 02 , P < 0 . 05 ) . ^^^ CONCLUSIONS : Balloon angioplasty upregulated Mac 1 ( CD11b / CD18 ) on the surface of the neutrophils and increased sICAM 1 levels in association with late loss increase . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Third , maturation from imDC to maDC efficiently silenced expression of beta 2 integrins CD11b , CD11c , and CD 18 by maDC . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Antibodies to LFA 1 ( CD11a ) , Mac 1 ( CD11b ) or CD 18 reduced neutrophil accumulation by 62 percent , 59 percent and 86 % , respectively , while anti VLA 4 was without effect . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The expression of amino peptidase N ( CD 13 ) , C3bi receptor ( CD11b ) , and the neutrophil beta 2 integrin unit ( CD 18 ) did not change during the administration of G CSF , but that of both CD 13 and CD 18 increased 3 days after the last dose . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Surface expression of beta 2 integrins was measured by flow cytometry after incubating with labeled antibodies to CD11b and CD 18 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In another series of experiments , blocking monoclonal antibodies to both neutrophil CD11b and CD 18 were incubated with polymorphonuclear leukocytes after removal from the circuit before the adhesion assay . ^^^ Monoclonal antibodies to both CD11b and CD 18 significantly inhibited the adhesion of polymorphonuclear leukocytes at endothelial cells after 120 min of circulation . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Using a binding assay with 125I labeled fimbriae , we observed that fimbrial binding to the macrophages was inhibited by treatment with CD11a , CD11b , CD11c , or CD 18 antibody but not by that with CD 29 antibody . ^^^ Furthermore , Northern blot analyses showed that the fimbria induced expression of IL 1beta and TNF alpha genes in the cells was inhibited strongly by CD 18 antibody treatment and slightly by CD11a , CD11b , or CD11c antibody treatment . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The expression of intercellular adhesion molecule 1 ( ICAM 1 ) , the ligand leucocyte function antigen 1 ( LFA 1 , CD11a ) , and complement receptor type 3 ( CR 3 , or Mac 1 , CD11b ) has been studied in murine kidneys acutely infected with the fatal malaria parasite Plasmodium berghei ANKA . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Flow cytometric analysis revealed that 99 . 1 + / 0 . 9 % of the subcloned cells were positive for CD11b and CD 18 , both of which are ligands of the intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
A monoclonal IgM , specifically recognizing both CD11b and CD 18 of human neutrophils , was used to examine the organization and mobility of CD11b / CD18 in the plasma membrane of human neutrophils degranulated by dihydrocytochalasin B ( dhCB ) treatment and fMet Leu Phe ( fMLF ) stimulation . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
RESULTS : Ligation of selectin L , CD 18 and CD11b integrin subunits by monoclonal antibodies induced a rapid release of 24 . 6+ / 1 . 8 % ( p < 0 . 005 ) , 24 . 0+ / 2 . 9 % ( p < 0 . 001 ) and 22 . 7+ / 2 . 0 % ( p < 0 . 005 ) of total neutrophil gelatinase , respectively as compared with 11 . 1+ / 1 . 6 % in the control . ^^^ CONCLUSIONS : Ligation of selectin L and integrin CD11b / CD 18 provides stimulatory signals to neutrophils which induce secretion of gelatinase B that may facilitate their transmigration into sites of inflammation . . ^^^ Ligation of selectin L and integrin CD11b / CD18 ( Mac 1 ) induces release of gelatinase B ( MMP 9 ) from human neutrophils . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The surface expression of CD11a , CD11b , CD 18 and VLA 4 adhesion molecules on normal human eosinophils were not modulated by eotaxin stimulation . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The expression of CD11b and CD 18 on neutrophils was also detected with flow cytometry . ^^^ In addition , both CD11b ( MFI ) and CD 18 ( MFI ) correlated with Annexin 5 ( MFI ) on neutrophils . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Studies with specific antibodies showed that monocyte adhesion to platelets was mainly mediated by P selectin and monocyte PSGL 1 ( maximum inhibition 90 % ) . beta 2 Integrin blocking CD 18 and CD11b antibodies partly inhibited the arrest of rolling cells . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Neutrophil accumulation in tissues requires their adherence to vascular endothelial cells and involves the beta 2 integrin , CD11b / CD18 ( Mac 1 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Prednisolone also increased the ingestion of C3b opsonized yeast cells , while the expression of CR 3 receptors ( CD11b CD 18 ) measured by flow cytometry was unchanged . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
TIMs were then separated from tumor cells and other host cells by magnetic activated cell sorting and CD11b ( Mac 1 ) microbeads . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We investigated the expression of adhesion molecules ( CD11a , CD11b , CD 18 , CD 31 , CD 54 , and CD62L ) on neutrophils from nasopharyngeal aspirates ( NPAs ) and peripheral blood ( PB ) of infants with respiratory syncytial virus ( RSV ) induced bronchiolitis . ^^^ The expression of CD 31 and CD62L on neutrophils from NPAs is decreased and the expression of CD11b , CD 18 , and CD 54 on neutrophils from NPAs is increased compared with cells from PB of RSV infected infants . ^^^ Shedding of CD 31 and CD62L on neutrophils in RSV infection may contribute to the neutrophil emigration from blood to airways ; the upregulation of Mac 1 ( CD11b / CD18 ) and CD 54 on neutrophils may help explain the high percentage of neutrophils in the airways of RSV bronchiolitis ; and the upregulation of Mac 1 may be involved in the increased neutrophil airway epithelial adhesion in RSV infection . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
CD 10 , CD11b , CD11c , CD 13 , CD 18 , CD 35 , CD 45 , CD66acde , and CD66b were upregulated at 15 min and remained upregulated at 180 min . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Interaction of the fungal pathogen Candida albicans with integrin CD11b / CD18 : recognition by the 1 domain is modulated by the lectin like domain and the CD 18 subunit . ^^^ In this study we have investigated the roles of three regions within the receptor , the inserted ( 1 ) and lectin like domains within the CD11b subunit , and the CD 18 subunit , in CD11b / CD18 C . albicans interactions . ^^^ We report four major findings . 1 ) A mutation in CD 18 exerts a dominant negative effect on the function of the CD11b / CD18 complex . ^^^ This interpretation is based on the observation that in the absence of CD 18 , the CD11b subunit alone binds C . albicans well , but a single point mutation at Ser 138 of CD 18 abolishes CD11b / CD18 binding of the fungus . 2 ) The lectin like domain is not sufficient for CD11b / CD18 C . albicans interactions . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
However , it was inhibited by approximately 75 % by monoclonal antibodies to CD49d ( VLA 4 ) or CD11a ( LFA 1 ) but was not significantly affected by an antibody to CD11b ( Mac 1 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Flow cytometric analysis for CD11a , CD11b , and CD 18 expression was examined . ^^^ RESULTS : Ibudilast had an inhibitory effect on beta 2 integrin expression induced by PAF [ CD11a : 84 . 8 % versus 73 . 1 % ( preincubation with ibudilast ) , CD11b : 35 . 8 % versus 26 . 2 % , CD 18 74 . 9 % versus 65 . 6 % ] . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Tissue destruction is accompanied by infiltration with Mac 1 ( CD11b ) positive monocytes / macrophages , Mac 1 positive T cells , and expansion of CD8+ T cells . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
On the other hand , expression of L selectin ( CD62L ) on polymorphonuclear leukocytes , and LFA 1 , CR 3 ( CD11b ) and VLA 4 ( CD49d ) on monocytes was decreased . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In order to test this possibility , endotoxin induced neutrophil beta 2 integrins CD11b and CD 18 expression , phagocytosis , and hydrogen peroxide generation were examined in normal and HIV 1 Tat transgenic mice in the absence and presence of ethanol intoxication . ^^^ The baseline expression of CD11b and CD 18 was similar in normal nontransgenic and Tat transgenic mice . ^^^ Endotoxin administration significantly up regulated CD11b and CD 18 expression in normal mice . ^^^ Ethanol intoxication inhibited endotoxin induced CD11b and CD 18 expression in normal mice and totally abolished endotoxin induced CD11b and CD 18 expression in Tat transgenic mice . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We induced beta 2 integrin cross linking by plating PMN onto surface bound mAbs directed against either the common beta chain ( CD 18 ) or the individual alpha chains ( CD11a , CD11b , CD11c ) of LFA 1 , CR 3 , and gp150 / 95 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
During reperfusion , CD11b and CD 18 , but not CD11a , were upregulated on neutrophils [ bronchoalveolar lavage ( BAL ) and blood ] and lung macrophages . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
This study revealed an upregulation of CD11b ( Mac 1 ) membrane expression on circulating PMN from patients with CD , as assessed by the mean fluorescence intensity which reflects antigen density . ^^^ In conclusion , blood PMN from patients with CD showed an enhanced capacity to be recruited into inflammed intestinal mucosa , which could be due to an increased expression of CD11b ( Mac 1 ) as well as an increased chemotactic response toward fMLP or IL 8 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Glomerular volume , sclerosis in glomeruli ( segmental and global ) and interstitium ( semiquantitative scale ) , infiltrating lymphocytes and macrophages ( CD 43 and ED 1 positive cells ) , and expression of adhesion molecules ( CD 54 , CD 18 , and CD11b ) were analyzed . ^^^ Expression of adhesion molecules CD 18 and CD11b was similarly reduced . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Despite the high level of cellular activation and proliferation induced by PMVEC coculture , the surface expression of adhesion molecules CD11a ( LFA 1 ) , CD11b , CD15s ( sialyl Lewis 10 ) , CD 43 , and CD 44 ( HCAM ) on the total CD34+ population was maintained , and the surface expression of CD49d ( VLA 4 ) , CD 54 ( ICAM ) , CD 58 , and CD62L ( L selectin ) increased after ex vivo expansion . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Immunohistochemistry was performed using the APAAP method and directing specific mouse anti human monoclonal antibodies against ICAM 1 ( CD 54 ) , VCAM 1 ( CD 106 ) , E selectin ( CD62E ) , alpha LFA 1 ( CD11a ) , alpha Mac 1 ( CD11b ) , alpha p150 / 95 ( CD11c ) and the beta 2 integrin chain ( CD 18 ) . ^^^ CD 18 , LFA 1 ( CD11a ) , Mac 1 ( CD11b ) and p150 / 95 ( CD11c ) were markedly expressed on infiltrating immunocytes in HTX compared to CABG where no expression of beta 2 integrins was observed . ^^^ CD 18 , LFA 1 ( CD11a ) , Mac 1 ( CD11b ) and p150 / 95 ( CD11c ) were markedly expressed on infiltrating immunocytes in HTX compared to CABG where no expression of beta 2 integrins was observed . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In contrast , DC expression of leucocyte function associated 1 ( LFA 1 , CD11a ) , Mac 1 ( CD11b ) , integrin beta 2 chain ( CD 18 ) and the DEC 205 receptor increased , while CD 40 levels were relatively unchanged 24 h after the treatment . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
RESULTS : NK cell adhesion to TNF a treated PAEC increased in a dose dependent manner by a maximum of 44 % , and was inhibited by mAbs against CD49d , CD11a , CD11b , CD 18 , and CD 2 , as well as porcine vascular cell adhesion molecules . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Neutrophil expression of CD11a , CD11b , and CD 18 was monitored by flow cytometry . ^^^ An increase in CD11b and CD 18 and a decrease of CD11a on neutrophils in parallel with complement activation was observed . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The labelling intensity of anti human CD11b , CD 18 and CD 32 mAb on the granulocytes in dogs with ehrlichiosis was similar to that in control dogs . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Regulation of MAC 1 ( CD11b / CD18 ) expression on circulating granulocytes in endurance runners . ^^^ METHODS : In eight amateur runners , MAC 1 ( CD11b / CD18 ) surface expression on granulocytes was determined by fluorescent antibody cell sorting , before and after an incremental maximal treadmill test , a moderate 3 h run , and a competitive marathon race . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In addition , a significant reduction in the CD11b and CD 18 positive cells and the amount of interstitial neutrophil infiltration in the lungs from WS 4 treated animals was seen . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The expression of the beta 2 integrins CD11a , CD11b and CD 18 , of CD 35 ( CR 1 ) , and of Fc gamma RII and Fc gamma RI ( CD 32 and CD 64 ) on monocytes was elevated in the RA patients compared with healthy controls , while the expression of the beta 1 integrins ( CD 29 , CD49d , CD49f ) was unaffected . ^^^ After 4 6 weeks of treatment with low dose prednisolone , the expression on the monocytes of CD11a , CD11b , CD 18 , CD 35 , CD 32 and CD 64 was normalized . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The CD1a+ cells reacted with antibodies against protein S 100 , HLA DR , the Lag antigen , CD 4 , CD11a , CD11b , CD 18 , CD 25 , CD 40 , CD 54 , CD 80 and CD 86 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Allografts from mice treated with CP 105696 at 50 or 100 mg / kg / day demonstrated a selective reduction in beta 2 integrin ( Mac 1 ) expression on monocytes / macrophages , as demonstrated by CD11b staining density compared with allograft controls . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
BCG immunization was also associated with increased peripheral lymphocyte and monocyte activation , as evidenced by increased surface expression of IL 2 receptor ( CD 25 ) ( P < 0 . 02 ) and MAC 1 ( CD11b ) ( P < 0 . 05 ) , respectively . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We examined the effect of plaunotol on neutrophil elastase release , production of O 2 , intracellular calcium concentration and expression of adhesion molecules CD11b and CD 18 in activated neutrophils in vitro . ^^^ The fMLP induced increases in CD11b and CD 18 expression were also inhibited by plaunotol in vitro . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Using flow cytometry , the expression of alpha 4 , CD11b , and CD 18 was upregulated in BAL , but not in blood , eosinophils . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Interestingly , an inhibitory population of adherent CD11b ( Mac 1 ) / Gr 1 double positive cells caused the observed impairment of CD8+ T cell function upon direct cell to cell contact . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We have also tested the effect of beta endorphin and met enkephalin on CD11a , CD11b , CD 18 and CD 16 molecule expression on PMN in vitro . ^^^ To assess the effect of opioid peptides on CD11a , CD11b , CD 18 and CD 16 molecule expression the whole blood samples were incubated with different concentrations of the opioids , then the white cells were labelled with respective PE conjugated MoAb and evaluated by flow cytometry . ^^^ We have shown that : ( 1 ) met enkephalin and beta endorphin at physiological concentrations relevant to that of in vivo ( 10 ( 8 ) and 10 ( 6 ) M ) enhanced fMLP , PMA or OZ stimulated chemiluminescence and induced chemotactic response , ( 2 ) High concentrations of beta endorphin ( 10 ( 3 ) M ) or met enkephalin ( 10 ( 5 ) M ) decreased the CL response of PMN in vitro , ( 3 ) The opioid peptides at lower concentrations resulted in CD11b and CD 18 molecule up regulation on neutrophils . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
These data suggest that the lectin site may be located entirely within CD11b , although lectin site dependent signaling through CD 18 probably occurs with the heterodimer . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Antibodies to CD11b / CD 18 receptors ( macrophage Mac 1 receptors ) reduced phagocytosis of particles by 50 percent ( p < 0 . 05 ) . ( The CD11b / CD18 macrophage receptor is the macrophage receptor for the complement component CR3bi . ^^^ Exposure of macrophages to either titanium alloy particles or to antibodies to the receptor proteins CD11b and CD 18 for thirty minutes activated the transcription factors NF kappaB and NF IL 6 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
These results suggest that alpha Toc may prevent inflammation by both reducing CD11b / CD 18 up regulation and decreasing PMN dependent adherence to EC . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
CasBrE envelope expression within microglia was accompanied by increased expression of activation markers F4 / 80 and Mac 1 ( CD11b ) but failed to induce spongiform neurodegenerative changes . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The effect of recombinant bovine somatotropin ( bST ) on the chemiluminescence , diapedesis , and expression of adhesion receptors ( CD11a , CD11b , CD 18 ) of isolated polymorphonuclear leukocytes was studied . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
METHODS : Neutrophil superoxide anion production was determined by lucigenin enhanced chemiluminescence ; the release of secondary granule lactoferrin by ELISA ; and the expression of cell surface adhesion molecules CD11b , CD 18 and L selectin ( CD62L ) by flow cytometric analysis . ^^^ There were no differences between the three study groups in the plasma levels of lactoferrin , or in the stimulated expression and release of CD11b and CD 18 , or lactoferrin and L selectin . ^^^ The base line levels for the production of superoxide anions ; the expression of CD11b or CD 18 or L selectin ; and the release of lactoferrin showed no significant differences . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
PMN apoptosis was assessed every 6 hours with use of propidium iodide DNA staining and PMN function was assessed with use of respiratory burst activity , phagocytosis ability , and CD11a , CD11b , and CD 18 receptor expression as functional markers . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Flow cytometry was used to determine PMN receptor expression ( CD11b , CD 14 , CD 16 , CD 18 , CD 54 ) , phagocytosis , and apoptosis . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : We tested the ability of the EG MPHA to detect granulocyte antigens using 5 human antibodies specific for NA 1 , NA 2 , NB 1 , 5b , and Sar ( a ) , and 8 different monoclonal antibodies for NA 1 , CD11a , CD11b , CD 13 , CD 16 , CD 18 and HLA class 1 . ^^^ RESULTS : NA 1 , NA 2 , NB 1 , 5b , Sar ( a ) , CD11a , CD11b , CD 13 , CD 16 , CD 18 and HLA class 1 antigens were present in the extracted granulocyte antigen preparation . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
CD11b , CD 18 , and CD 47 expression on PMN was compared before and after transepithelial migration by flow cytometry analysis . ^^^ Basal and transepithelial migration mediated expression of CD11b , CD 18 , and CD 47 were unmodified in HIV PMN compared to control PMN . ^^^ This defective migration is not due to a quantitative modification of CD11b , CD 18 and CD 47 on HIV PMN suggesting a more subtle alteration . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The purified proteins reacted specifically with monoclonal antibodies specific for CD11a , CD11b , CD11c ( the alpha chains of beta ( 2 ) integrins ) , and CD 18 ( the beta chain of beta ( 2 ) integrins ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Whereas expression of growth factor receptors merely allows a PU . 1 deficient cell line to survive and grow in the relevant growth factor , expression of PU . 1 enables the development of F4 / 80 ( + ) , Mac 1 ( + ) / CD11b ( + ) macrophages , expression of gp 91 ( phox ) and generation of superoxide , and expression of secondary granule genes for neutrophil collagenase and gelatinase . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
On days 3 , 5 , 7 , and 10 after the injection , splenocytes were harvested from both groups , and the following cell phenotypes were quantified by flow cytometry : ( 1 ) B cells ( CD45R / B220 ) ; ( 2 ) T cells ( CD3e ) ; ( 3 ) T cell subsets ( CD 4 and CD8a ) ; ( 4 ) natural killer ( NK ) cells ( NK 1 . 1 ) ; ( 5 ) macrophages ( CD11b ; Mac 1 ) ; ( 6 ) nucleated erythrocytes ( Ter 119 ) ; and ( 7 ) dead cells with propidium iodide ( PI ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We analyzed the role of ICAM 1 , CD 18 , CD11a , CD11b , and CD11c during episodes of rejection or infection in patients after lung transplantation and compared the results to episodes without apparent complication . ^^^ ICAM 1 , CD 18 , CD11a , CD11b , and CD11c expressions were detected immunocytochemically on alveolar macrophages . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Using flow cytometry , we found an increased expression of adhesion molecules CD62L , CD 18 , CD11a , CD11b , CD49d and CD 54 in the exposed workers as compared with the control group ( P < 0 . 05 ) . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Treatment with low dose prednisolone reduced the expression of CD11b on neutrophils and of CD11b , CD 18 and CD66b on eosinophils to the same level as that found in healthy controls . ^^^ Analyses by flow cytometry showed elevation of the neutrophil and eosinophil expression of the alpha and beta chains of the beta 2 integrin Mac 1 ( CD11b / CD18 ) and of the CEA gene family member 6 ( CGM 6 , CD66b ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The blood was incubated with neutralizing monoclonal antibodies to CD 18 , CD11a , CD11b , CD 29 , CD49d , CD 54 , alpha4beta7 , or isotype matched control antibodies , respectively , at 4 degrees C for 30 min . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
During ex vivo expansion of CD34+ cells isolated from human CB for up to 5 weeks , surface expression of molecules on the cultured cells including CD 64 ( Fc gammaRI ) , CD 32 ( Fc gammaRII ) , CD 16 ( Fc gammaRII ) , CD11b ( MAC 1 ) and CD 18 ( beta 2 integrin ) was analysed by flow cytometry along with simultaneous measurement of apoptosis by 7 aminoactinomycin D staining method . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Although the central role of beta 2 integrin CD11b / CD 18 in neutrophil functions is well recognized , signaling pathway that regulate integrin activation remain to be elucidated . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
These two cell types are derived from separate hematopoietic lineages and display distinct surface markers , for instance , the pan B cell marker B 220 ( CD45R ) and the myelo / monocytic marker Mac 1 ( CD11b ) , respectively . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The number of eosinophils , the level of eosinophil cationic protein ( ECP ) , the bindings of soluble intercellular adhesion molecule 1 ( sICAM 1 ) and fibronectin ( FN ) , and the expressions of CD11b ( alpha chain of Mac 1 ) and CD49d ( alpha chain of VLA 4 ) on eosinophils were evaluated in peripheral blood ( n=4 ) and BALF ( n=3 ) before and after the administration of Y 24180 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Here we investigated major adhesion molecules as predisposition factors via common polymorphisms in or in the vicinity of the candidate genes ICAM 1 , e selectin , PLAUR , CD11b , and CD 18 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Phorbol 12 myristate 13 acetate and all trans retinoic acid , which induce differentiation in U 937 cells , up regulated CD11b ( MAC 1 alpha integrin ) and CD 82 and down regulated CD 71 ( transferrin receptor ) in a time and dose dependent manner . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Several integrin molecules potentially involved in this process , i . e . , CD 29 , CD 18 , CD11a , CD11b and CD11c , were therefore studied by fluorescence activated cell sorting analysis . ^^^ Among the beta 2 integrins , the expression of LFA 1 ( CD11a ) and of Mac 1 ( CD11b ) , but not of p 150 , 95 ( CD11c ) , was induced by RA . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Effect of anti CD11b ( alphaM MAC 1 ) and anti CD 54 ( ICAM 1 ) monoclonal antibodies on indomethacin induced chronic ileitis in rats . ^^^ This study investigated the effect of monoclonal antibodies against CD 54 ( ICAM 1 ) and CD11b ( alphaM chain of MAC 1 ) on intestinal inflammation . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The studies revealed that the integrins CD11a , CD11b , CD 18 , CD49d , and CD 61 , as well as the additional adhesion receptors CD 54 , CD62L , and the transferrin receptor CD 71 were expressed on day 11 of gestation to various degrees on these embryonic cells . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Immunoreactivity of postreperfusion cardiac lymph with R15 . 7 ( anti CD 18 ) or MY 904 ( anti CD11b ) was positive in all animals but not for CD11a ( R7 . 1 ) , indicating a specific loss of Mac 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We have studied binding of fluorescein isothiocyanate ( FITC ) stained TNF alpha by PMN , the expression of CD11a , CD11b , and CD 18 molecules of beta 2 integrines and the expression of CD 10 ( neutral endopeptidase NEP ) and of CD 13 ( aminopeptidase N ; APN ) antigens on PMN in three different groups of MS patients . ^^^ The obtained results have proved that during MS exacerbation and in the course of chronic progressive MS , PMN reveal several forms of preactivation , including significantly higher stained TNF alpha binding , higher expression of CD11b and CD 18 , as well as CD 10 and CD 13 antigens , in comparison with MS remission or OND . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Compared with non TBI and non BMT mice , chimeric mice had an increased percentage of CD11b ( Mac 1 ) positive splenocytes ( 17 vs . 59 % , P < 0 . 05 ) and decreased CD45R positive ( B ) cells ( 33 vs . 6 % , P < 0 . 05 ) at 6 weeks after BMT . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The antigens recognized by R 1 73 , R 2 40 and R 1 34 mAbs were defined by immunoprecipitation and Western blot analyses as CD11a , CD 18 and CD11b , respectively ; and the allografts expressed CD 54 , a ligand of CD11a or CD11b , suggesting leukocyte integrin dependent killing . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Neutrophil migration across mammary arterial endothelial cells was almost completely dependent on CD 18 , the beta chain of the beta ( 2 ) integrins , and to a lesser extent on CD11b , one of the alpha chains of the beta ( 2 ) integrins . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
CD11a , CD11b and CD 18 , measured by flow cytometry , were elevated in the non surviving group , the septic shock group and the multiple organ dysfunction syndrome group , suggesting a close connection between these adhesion molecules and burns complicated by infection . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
One such protein , the glycolipid anchored urokinase receptor ( uPAR ) , associates with and modifies the function of the beta ( 2 ) integrin Mac 1 ( CD11b / CD18 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The expression of the surface integrin subunits CD11a , CD11b , and CD 18 on rat neutrophils isolated from peripheral blood was quantitated with flow cytometry . ^^^ The adhesive role of the integrin subunits CD11a , CD11b , and CD 18 was tested using specific neutralizing monoclonal antibodies . ^^^ RESULTS : Neutrophil CD11a , CD11b , and CD 18 surface integrin levels were 62 % ( n = 5 , P = 0 . 006 ) , 54 % ( n = 5 , P = 0 . 045 ) , and 38 % ( n = 5 , P = 0 . 009 ) greater in diabetic versus nondiabetic animals , respectively . ^^^ Pretreatment of leukocytes with either anti CD11b or anti CD 18 antibodies lowered the proportion of adherent diabetic neutrophils by 41 % ( n = 6 , P = 0 . 01 for each treatment ) , whereas anti CD11a antibodies had no significant effect ( n = 6 , P = 0 . 5 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
CR 3 ( CD11b / CD18 ) , a beta ( 2 ) integrin , has a key role in innate antimicrobial defenses , as evidenced by the leukocyte adhesion ( CD 18 ) deficiency syndrome in humans and the CD11b knockout mouse . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
These markers included CD 2 , CD11b , CD11c , CD 18 , CD 16 , CD 54 , CD 56 , CD 98 , CD 25 , CD 122 , HLA class 1 , HLA class 2 , CD 94 , ILT 2 , p58 . 1 , p 70 , and NKp 46 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Neutrophil surface expression of CD11b and CD 18 and endothelial cell intercellular adhesion molecule 1 ( ICAM 1 ) and vascular adhesion molecule 1 ( VCAM 1 ) were assessed by flow cytometry and an enzyme immunoassay , respectively . ^^^ RESULTS : The expression of CD11b and CD 18 on neutrophils and neutrophil dependent adhesion to endothelial cells elicited by HPE were inhibited by lansoprazole and omeprazole at clinical relevant doses , and the expression of ICAM 1 and VCAM 1 on endothelial cells and endothelial dependent neutrophil adherence induced by IL 1beta were also inhibited by lansoprazole and omeprazole at similar doses . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Phenotypic studies combined with data on intracellular iNOS expression and cell sorting indicate that NO producing cells are CD 40 , CD 31 ( ER MP 12 ) , CD11b ( Mac 1 ) low , ER MP 20 ( Ly 6C ) and Gr 1 ( Ly 6G ) positive , consistent with myeloid progenitors . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We also examined the following antigens as indicative of activation and adhesion of the monocytes in these patients : CD11b , CD 18 , CD 35 , CD 38 , CD 44 , CD 69 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
OBJECTIVES : This study was designed ( 1 ) to evaluate the influence of plasma obtained from patients undergoing coronary artery bypass grafting on L selectin , CD11b , and CD 18 expression on human neutrophils and ( 2 ) to determine the influence of the use of crystalloid or blood cardioplegia during bypass grafting on plasma mediated expression of adhesion molecules on polymorphonuclear neutrophils . ^^^ Human neutrophils were incubated with patients ' plasma , and the expression of CD11b , CD 18 , and L selectin was determined with flow cytometry . ^^^ RESULTS : In patients receiving crystalloid cardioplegic solutions , plasma samples collected from the coronary sinus at aortic clamp release and 30 minutes thereafter induced significantly higher expression of neutrophil CD11b and CD 18 than plasma samples obtained from a peripheral vein or artery at the same time points . ^^^ In the group receiving blood cardioplegia , no significant differences in CD11b , CD 18 , or L selectin expression were found . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We demonstrate that ligation of CD11b ( Mac 1 , CR 3 ) or CD11c ( p 150 , CR 4 ) alpha chains of beta 2 integrins by mAbs or soluble chimeric CD 23 ( sCD 23 ) on human freshly isolated monocytes rapidly stimulates high levels of interleukin 1beta production . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We found that monoclonal antibodies against CD11a , CD11b , CD 18 , or LW ( ab ) block adhesion of transfectant L cells to immobilized ICAM 4 Fc protein and that the ICAM 4 / beta ( 2 ) integrin interaction was highly sensitive to the presence of the divalent cations Ca ( 2+ ) and Mg ( 2+ ) . ^^^ The red cell ICAM 4 / LW blood group glycoprotein , which belongs to the family of intercellular adhesion molecules ( ICAMs ) , has been reported to interact with CD11a / CD18 ( LFA 1 ) and CD11b / CD18 ( Mac 1 ) beta ( 2 ) integrins . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In addition , we analysed ( by flow cytometry ) whether this function correlated with modifications of the CD 2 , CD11a , CD11b and CD 18 molecules , which are involved in the adhesion of effector cells to the counter receptors ( CD 54 and CD 58 ) on osteosarcomas . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Activation of Stat 3 leads to homotypic cell aggregation , increased expression of intercellular adhesion molecule 1 ( ICAM 1 ) , CD 18 , and CD11b , and activation of signaling through CD 18 containing integrins . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Superoxide anion production ( respiratory burst ) was determined by lucigenin enhanced chemiluminescence ( LUCL ) ; secondary granule lactoferrin release by enzyme linked immunosorbent assay ( ELISA ) ; and CD11b , CD 18 , and CD62L expression by flow cytometric analysis . ^^^ Basal and stimulated CD11b , CD 18 , and CD62L expression showed no variations throughout gestation for both pregnancy groups . ^^^ Moreover , resting neutrophils and stimulated cells from patients with RA , including pregnant subjects , showed a marked increase in LUCL , but a reduction in CD11b , CD 18 , and CD62L . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
CD 13 Ag and CD11a , CD11b , CD 18 molecule expression on peripheral blood mononuclear cells ( PBMC ) were studied as these cells ' adherent or transendothelial migration properties in three different multiple sclerosis ( MS ) patients groups ( total 38 ) : with clinically active MS ( acute exacerbation of MS and primary chronic progressive MS ( CP MS ) ) and with MS remission . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
There were significantly fewer cells expressing CD11b , CD 18 and CD 29 in the CD 64 DCp population compared with CD 14 DCps , and this CD 64 DCp subpopulation also had a lower expression of CD11b and CD 18 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In addition , anti GM CSF MoAb , but not anti VEGF or anti IL 8 MoAb , reduced PMN CD11b and CD 18 overexpression induced by tumour conditioned medium ( p < 0 . 05 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
CD11b is an alpha chain of the leukocyte beta ( 2 ) integrin , Mac 1 , which mediates binding and extravasation of leukocytes . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
To define the influence of IAT on leukocytes , we determined leukocyte L selectin ( CD62L ) and Mac 1 ( CD11b ) as parameters for activation of leukocytes in peripheral blood during IAT . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Additionally , the appearance of several activation ( CD 25 , CD 69 , HLA DR ) and adhesion ( CD11a , CD11b , CD11c , CD 18 , CD49b , CD49d , CD 54 ) receptors on CD 4 cells were analyzed . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In addition , the surface markers alpha 2 , alpha 3 , alpha 4 , CD11a , CD11b , CD 18 , CD 54 , and CD 8 found on lymphocytes and alpha 3 , alpha 4 , CD11a , CD 18 , and CD 8 surface markers found on granulocytes were also decreased in the patient population . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies against CD11a , CD 18 and CD 54 , but not CD11b , restored eosinophil suppression of T lymphocyte proliferation which was chymostatin resistant in coculture . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies ( mAbs ) directed against CD 14 , CD11b , CD 18 or urokinase receptor ( uPAR ) significantly inhibited PTX induced primed U 937 cell adhesion in serum in a concentration dependent manner . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Serial colonic sections ( 10 UC , 10 CD , and 10 controls ) were stained immunohistochemically for ICAM 1 , ICAM 2 , ICAM 3 , CD11a , CD11b , CD 18 , and PECAM 1 . ^^^ In conclusion , CD11b , CD 18 , and ICAM 2 seem to be important for PMN transepithelial migration in UC , whereas CD11a , CD11c , ICAM 1 , and ICAM 3 seem central in leukocyte locomotion and aggregation in CD . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
When peripheral blood mononuclear cells ( PBMC ) obtained from healthy donors were cultured in the presence of culture supernatants of KC separated from the noninflamed gingivae ( Nor KC ) and cheek mucosae of patients with OLP ( OLP KC ) , the number of migrated PBMC across monolayered human umbilical vein endothelial cells ( HUVEC ) were increased to about 1 . 3 fold and 1 . 5 fold of the control level , respectively , with increases of the expression of CD11a , CD11b , CD 18 , and CD49d on PBMC and intracellular adhesion molecule 1 , vascular cell adhesion molecule 1 , and endothelial leukocyte adhesion molecule 1 on HUVEC . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We show that the CD14+CD16++ cells express dendritic cell markers : CD 40 , CD 80 , CD 86 , HLA DR , CD11b , CD11c , CD 18 , CD1a , and CD 83 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
CD11b and CD 18 expression in PECs was increased significantly after CLP compared with PECs from non CLP challenged animals ( p < . 05 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
INTERVENTIONS : Effects of ranitidine on neutrophil elastase release , production of O 2 , intracellular calcium concentration and expression of adhesion molecules CD11b and CD 18 were examined in human neutrophils in vitro . ^^^ MEASUREMENTS AND MAIN RESULTS : Ranitidine inhibited the release of neutrophil elastase as well as the production of O 2 , the increase in the concentrations of intracellular calcium , a second messenger of neutrophil activation , and increases in CD11b and CD 18 expression , in activated neutrophils . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
CONCLUSION : Patients on haemodialysis have an increased systemic chemotactic activity for monocytes , unphysiological phenotypic alterations in CD11b / CD 18 expression during and after dialysis , and increased sVCAM 1 and MCP 1 concentrations . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We found that the cross linking of CD11b as well as of CD 18 induced comparable rapid increases in CD 64 expression on the surface of PMNs , occurring within 15 minutes of exposure . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In contrast , aprotinin inhibited the cardiopulmonary bypass induced increase in monocyte ( p < 0 . 05 ) and granulocyte ( p < 0 . 01 ) CD 18 and the increase in granulocyte , but not monocyte , CD11b ( p < 0 . 05 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In the present study , we investigate the effects of antibodies against CD11b and CD 18 , as well as HDL 3 and low density lipoprotein ( LDL ) cholesterol on fibrinogen binding on monocytes . ^^^ We find that binding of fibrinogen on monocytes activated with adenosine diphosphate ( ADP ) was reduced to 66 . 0+ / 8 . 3 % ( mean + / SD ) in the presence of anti CD11b antibodies ( 12 . 5 microg / ml ; P < or = 0 . 02 ) and to 54 . 5+ / 4 . 9 % in the presence of anti CD 18 antibodies ( 20 microg / ml ; P < or = 0 . 01 ) , respectively . ^^^ Neither incubation with HDL 3 or with LDL had a significant influence on ADP activated cellular binding of anti CD11b or anti CD 18 antibodies . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
METHODS : MFI expression and percentage of CD 18 , CD11b , CD62L and CD 54 were evaluated resting and activated by 30 min . , fMLP preincubation using monoclonal antibodies anti CD11b and anti CD62L , employing Flow Cytometer FACSCalibur with computer program CELLquest . ^^^ RESULTS : Expression of CD11b and CD 18 receptor on leukocyte of A , B , D groups was higher spontaneous and after stimulation by fMLP in 1 st trimester compare to group C and then lowered in IIIrd . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Expressions of CD11b , CD 18 , and CD62L were measured by flow cytometry . ^^^ However , CD11b and CD 18 expressions on circulating PMNs were significantly lower in the diet restricted than in the ad libitum group . ^^^ The expressions of CD11b , CD 18 and CD62L on exudative PMNs were unaffected by dietary restriction . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
This correlated with increased expression of CD11b ( r= 0 . 93 ) and CD 18 ( r= 0 . 98 ) after incubation with 5 , 000 IU / ml G CSF ( P < 0 . 05 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Moreover , the differentiation status of these Raf responsive cells was more immature upon Raf activation as culture with the differentiation inducing agent phorbol 12 myristate 13 acetate ( PMA ) and beta estradiol resulted in decreased levels of the CD11b and CD 18 integrin molecules on the cell surface . ^^^ In contrast when the Raf responsive cells were induced to differentiate with PMA and GM CSF , in the absence of deltaRaf : ER activation , increased levels of the CD11b and CD 18 molecules were detected . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
However , neither DX 9065a , a selective inhibitor of Factor Xa , nor recombinant Factor VIIa treated with dansyl glutamylglycylarginyl chloromethyl ketone , a selective inhibitor of Factor VIIa , had any effects on LPS induced pulmonary vascular injury despite their potent anticoagulant effects . rTFPI significantly inhibited TNF alpha production by LPS stimulated monocytes in vitro . rTFPI also significantly inhibited several formyl Met Leu Phe induced neutrophil functions , as well as increases in the expression of CD11b and CD 18 on the neutrophil cell surface in vitro . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
On milk derived neutrophils , the expression of CD11b and CD 18 was upregulated compared to blood , in contrast to CD62L that was downregulated . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In these subjects we determined , at baseline and after in vitro activation with 4 phorbol 12 myristate 13 acetate and N : formyl methionyl leucyl phenylalanine , the polymorphonuclear leukocyte membrane fluidity , obtained by labeling the cells with 1 [ 4 ( trimethylamino ) phenyl ] 6 phenyl 1 , 3 , 5 hexatriene , cytosolic Ca ( 2+ ) concentration , obtained by marking the cells with Fura 2 AM , and integrin pattern ( CD11a , CD11b , CD11c , and CD 18 ) , by using the indirect immunofluorescence with a flow cytometer . ^^^ At baseline there was no difference in membrane fluidity between normal subjects and hypertensives , whereas hypertensives showed an increase in cytosolic Ca ( 2+ ) content and an increase of the phenotypical expression of CD11a , CD11b , and CD 18 . ^^^ In normal subjects , after activation , we observed a significant increase of the expression of all adhesion molecules , whereas in hypertensives we found an increase of the expression of CD11b , CD11c , and CD 18 but also a decrease of CD11a . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Treatment of monocytes with antibody to CD 14 , CD 18 , or CD11b showed that CD 14 , but not CR 3 ( CD11b / CD18 ) , mediated monocyte TNF production in response to the soluble antigens . ^^^ In contrast , anti CD 14 , anti CD11b and anti CD 18 monoclonal antibodies all inhibited the response to the particulate stimuli . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
To elucidate the innate immune responses to group A streptococci ( GAS ) important in the pathophysiology of sepsis , flow cytometric techniques were applied to study the effects of live and heat inactivated GAS , including their particulate and soluble components , on the expression of leukocyte adhesion molecules CD11b ( Mac 1 ) and CD62L ( L selectin ) , and leukocyte production of reactive oxygen species ( ROS ) in human whole blood . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In this study , we used genetically modified bone marrow derived CD11b ( + ) CD 18 ( + ) vehicle cells to deliver IL 1 receptor antagonist ( IL 1ra ) for treatment of inflamed renal interstitium in an animal model of unilateral ureteral obstruction ( UUO ) . ^^^ Vehicle cells that expressed the ICAM 1 ligands , CD11b and CD 18 , were obtained from bone marrow cells of DBA / 2j mice and adenovirally transduced with the IL 1ra gene or glucocerebrosidase ( GC ) gene ex vivo . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Alveolar recruited monocytes showed a blood monocytic phenotype as assessed by cell surface expression of F4 / 80 , CD11a , CD11b , CD 18 , CD49d , and CD62L . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Impaired adhesion as well as transendothelial migration of monocytes derived from peripheral blood of psoriatic arthritis patients can be explained by the reduced expression of adhesion molecules MAC 1 ( CD11b / CD18 ) at the surface of monocytes . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We periodically drew blood from the dorsalis pedis vein and a brachial vein of 11 healthy test persons and 8 patients with stage 3 CVI during experimental venous hypertension in order to evaluate the expression pattern of leukocyte adhesion molecules involved in inflammation : LFA 1 ( CD11a ) , Mac 1 ( CD11b ) , p 150 , 95 ( CD11c ) , CD 18 , VLA 4 ( CD49d ) , and L selectin ( CD62L ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
All expressed high levels of CD11a ( LFA 1 ) , CD11b ( Mac 1 ) , and CD 54 antigens , with low levels of class 2 major histocompatibility complex . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Acquisition of the osteoclast phenotype [ the alphavbeta 3 subunit of the vitronectin receptor ( VNR ) , calcitonin receptor ( CTR ) and F actin ring positive cells ] is associated with loss of monocyte / macrophage associated integrins , specifically CD11b , CD11c and CD 18 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We analyzed the phenotype of CB monocytes in the presence or absence of an inflammatory signal ( rIFN gamma ) and compared them to adult blood ( AB ) ; the expression of HLA DR and 17 different markers ( CD11a , CD11b , CD11c , CD 18 , CD 29 , CD 40 , CD 44 , CD49a , CD49d , CD49e , CD49f , CD 54 , CD 58 , CD62L , CD 80 , CD 86 and CD 102 ) was measured by flow cytometry . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
At 14 weeks of culture , more mature CHMCs expressed CD11b , CD11c , CD 29 , CD49b , CD49c , CD49d , CD49e , CD 51 , CD 61 , and CD 54 and weakly expressed CD 18 and CD11a . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The expression of CD11b and CD 18 antigens on blood neutrophils was also significantly increased after peritonitis induction . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
MAIN OUTCOME MEASURES : CD11b , CD 18 , and CD62L expressions on circulating PMNs , phagocytosis , and reactive oxygen intermediate production by exudative PMNs were measured after glycogen installation . ^^^ Both CD11b and CD 18 expression on circulating PMNs and MIP 2 levels correlated significantly with numbers of exudative PMNs . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Isolated leukocytes were then analyzed for expression of the adhesion molecules L selectin ( CD62L ) , Mac 1 ( CD11b and CD 18 ) , and major histocompatability complex ( MHC ) class 1 and class 2 molecules with immunostaining and flow cytometric analysis . ^^^ Energy balance did not affect expression of CD62L on any other cell types or expression of CD11b or CD 18 . ^^^ The results of this study provide little evidence that nutritionally created negative energy balance impairs expression of CD62L , CD11b , and CD 18 or expression of MHC class 1 or MHC class 2 molecules by resting bovine blood leukocytes . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The expression of adherence receptors CD62L , CD 18 , and CD11b , which reflects the activation state of the neutrophils , was assessed in vitro by a direct immunofluorescence method using calibration beads measured by flow cytometry . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
FACScan analysis revealed that these tumor cells expressed high levels of intercellular adhesion molecule 1 ( ICAM 1 ) but did not express CD11a , CD11b , or CD 18 . ^^^ Blockage of CD11b and CD 18 on PMN and of ICAM 1 on MDA MB 231 cells significantly attenuated TCM treated , PMN mediated tumor cell migration . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Up regulation of CD11b and CD 18 on PMN was also induced by the immunoglobulin G ( IgG ) fraction of the sera of RV patients . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
RESULTS : Intestinal I / R resulted in lung injury characterized by an increase in Evans blue dye concentration , neutrophil sequestration , and obvious staining for expression of pulmonary CD11b and CD 18 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Antibodies specific to CD11b , CD 16 , CD 18 , CD62L , and CD 162 were used to block PMNL adhesion . ^^^ Mixing blood with antibodies to CD11b , CD 18 , and CD62L amplified the adhesion of PMNLs to the hydrophilic surface . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Clinical and hematological data , respiratory burst of neutrophils , and the expression of adhesion and activation molecules ( CD 18 , CD11b , CD 69 , and HLA DR ) were analyzed by flow cytometry . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The effect of infection of sheep with the gastrointestinal nematode parasite Trichostrongylus colubriformis on expression of adhesion molecules CD11a , CD11b , CD11c , CD 18 , CD 44 , CD49d and CD62L by peripheral blood eosinophils was examined by flow cytometry . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
MATERIAL AND METHODS : In vivo and in vitro studies were performed in 10 patients undergoing an MR imaging procedure , PMN adhesion to a plastic surface , as well as the expression of adhesion molecules beta 2 integrins CD11b , CD 18 , and L selectin on the surface of PMN were estimated . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We examined in 19 subjects with acute ischaemic stroke ( AIS ) the PMN integrin pattern ( CD11a , CD11b , CD11c , CD 18 ) , using indirect immunofluorescence and adopting a flow cytometer , at baseline and during activation , prolonged for 5 and 15 min , with 4 phorbol 12 myristate 13 acetate ( PMA ) . ^^^ At baseline , an increase in the expression of CD11c and CD 18 and a decrease in the CD11b were evident in AIS subjects compared to normals . ^^^ After activation , we found in normals a constant and significant increase of all PMN adhesive molecules , while in AIS subjects , we found an increase in CD11b and CD 18 , a decrease in CD11a and no variation in CD11c . ^^^ While the basal upregulation of CD11c and CD 18 may depend on the PMN spontaneous activation or on the increase of cytokines , the decrease of CD11b may be due to its self consumption . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Eosinophils and lymphocytes showed an activated state before treatment , characterized by marked expression of CD11b , CD 18 , CD45RO on eosinophils and increased HLA DR , CD 95 , CD 18 , CD 38 on lymphocytes . ^^^ Twenty four hours after administration of HDMP a dramatic reduction in peripheral blood eosinophil count was observed in both patients associated with phenotypic changes characterized by decreased expression of CD11b , CD 18 , CD 13 and increased CD 95 expression in one . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Physiological concentrations of ICAM 1 ( 0 . 05 500 pM ) were separated from neutrophils by nitrocellulose filters , and cell migration was evaluated using the leading front technique . beta 2 integrins on neutrophils were blocked with antibodies to CD11a , CD11b , CD11c and CD 18 , and migration towards ICAM 1 was examined . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Increased expression of the leukocyte integrins CD11b and CD 18 as well as down regulation of the anti apoptotic gene bcl 2 are associated with late stage differentiation of the myeloid lineage and retinoic acid induced maturation of acute myeloid leukemic cells . ^^^ Lovastatin exposure induced increased expression of CD11b and CD 18 markers similar to retinoic acid treatment . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The effect of circuit surface area on CD11b ( mac 1 ) expression in a rat recirculation model . ^^^ The aim of the study was to assess the effect of exposed surface area of diethylhexylphthalate plasticized polyvinylchloride ( PVC ) on the expression of the adhesion molecule CD11b ( mac 1 ) on neutrophils and to determine whether there is any apparent advantage in the current trend in reducing circuit surface area in terms of neutrophil activation . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
By flow cytometry , we also measured the expression of leukocyte CD11a , CD11b , CD11c , CD 18 , CD 31 , CD49d , and CD62L ( L selectin ) in 13 patients . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Flow cytometric analysis of membrane antigen density of neutrophils activated with bG CSF revealed an upregulation in the expression of CD11a ( > 114 % ) , CD11b ( > 148 % ) , CD11c ( > 87 % ) , and CD 18 ( > 109 % ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Antibodies against CD 18 , CD11b , or EDTA , the last of which chelates divalent cations required for integrin function , had no effect on adhesion of normal mouse or human macrophages to matrixes containing oxLDL but almost completely inhibited ROS production by macrophages adherent to this matrix . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We studied the expression of CD11b , CD 18 , CD 35 , CD 67 , CD 69 , CD 44 , and CD 53 molecules known to be changed in the activated form of neutrophils . ^^^ CD 43 expression correlated positively with CD 53 and CD 44 expression and negatively with CD11b , CD 18 , CD 35 , CD 67 , and CD 69 expression . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
RESULTS : The results show that incubation of CU membranes with the proteins CD11b , CD 18 , and CD 54 or the blockade of these cell surface molecules with specific monoclonal antibodies inhibited the CU induced aggregation and apoptosis in a dose dependent manner . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Incubation of PMNs with anti CD 16 prevented the up regulation of beta ( 2 ) integrins , particularly CD11b , which is the alpha chain of complement receptor type 3 , but also CD 18 , which is its beta chain , as well as CD11a and CD11c . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
It is predominantly expressed in immature cells of the myelomonocytic lineage and in a subset of CD11b ( Mac 1 ) positive cells in some tissues . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Flow cytometrical analysis demonstrated that L NAME induced an increased expression of CD11b ( Mac 1 ) on the eosinophil cell surface of 36 . 3+ / 7 . 4 % . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The mechanisms for these activities remain incompletely defined , although uPAR was recently identified as a cis acting ligand for the beta 2 integrin CD11b / CD18 ( Mac 1 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Neutrophil activation was assessed by level of CD11b and CD 18 expression after incubation with autologous antepartum or postpartum plasma . ^^^ Neutrophil CD11b and CD 18 expression was not differentially altered by incubation with plasma from either women with preeclampsia or normal pregnant women and was similar between groups when incubation was performed with plasma collected after delivery . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The authors previously reported that bone marrow derived CD11b ( + ) CD 18 ( + ) cells could be used as a vehicle to deliver foreign genes into inflamed glomeruli and that this vehicle cell ( 5 cell ) could retard the progression of nephritis by delivering anti inflammatory molecules . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Characterization of mAb 13 . 22 specificity by immunoblotting showed that it recognized proteins of MW approximately 95 000 and 150 000 , including both CD 18 and CD11b . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
They expressed CD 4 , CD11a , CD11b , CD11c , CD 18 , CD 32 , CD 33 , CD45R0 , CD 48 , CD 50 , CD 54 , CD 58 , CD 64 , CD 86 , CD 102 , CD 116 , CD 123 , and HLA DR ; showed no expression of CD1a and CD 83 ; and weak expression of CD 16 , CD45RA , and CD 80 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
CD 18 ( A270V ) supports , at a diminished level , CD11b / CD18 ( Mac 1 , alphaMbeta 2 integrin ) and CD11c / CD18 ( p 150 , 95 , alphaXbeta 2 integrin ) expression and function but not CD11a / CD18 ( LFA 1 , alphaLbeta 2 integrin ) expression . ^^^ CD 18 ( C590R ) and CD 18 ( R593C ) show a decreasing capacity to associate with the CD11a , CD11c and CD11b subunits . ^^^ CD 18 ( A270V ) supports , at a diminished level , CD11b / CD18 ( Mac 1 , alphaMbeta 2 integrin ) and CD11c / CD18 ( p 150 , 95 , alphaXbeta 2 integrin ) expression and function but not CD11a / CD18 ( LFA 1 , alphaLbeta 2 integrin ) expression . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Furthermore , the levels of VEGF secretion correlated with the expression of cell surface integrin ( CD11b and CD 18 ) on both monocytes and PMNs in the SF . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Overexpression of protein kinase C zeta ( PKC zeta ) in the leukemic myeloid cell line U 937 ( U 937 PKC zeta cells ) , previously shown to induce leukemic cell differentiation , resulted in nearly complete downregulation of leukocyte integrins CD11a , CD11b , CD11d , and CD 18 , but not CD11c from the cell surface . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Cell numbers were quantified with an automated counter and flow cytometry was used to quantify CD11b and CD 18 . ^^^ Although CD 18 levels were not different , CD11b was significantly higher on neutrophils from tail compared to heart blood . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Phenotypic and metabolic characteristics of monocytes and granulocytes were studied with the use of flow cytometry and monoclonal antibodies against surface markers ( CD11b , CD 14 , CD 15 , CD 16 , CD 18 , CD49d , CD62L , CD 64 , CD66b , and HLA DR ) . ^^^ Monocytes from patients with acute infection had a higher mean channel brightness for CD11b , CD 16 , CD 18 , CD49d , CD 64 , and CD66b than monocytes of normal pregnant women . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Maternal blood was assayed to determine granulocyte and monocyte phenotype by using monoclonal antibodies , which included the following cluster of differentiation ( CD ) markers : CD11b , CD 14 , CD 15 , CD 16 , CD 18 , CD49d , CD62L , CD 64 , CD66b , and HLA DR . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Comparing to control group adhesion molecules CD11b , CD 18 , CD 31 , CD49d , CD 54 and CD62L were increased on the surface of patient neutrophils whereas no differences were observed in the expression of CD11a abd CD 15 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
DESIGN AND METHODS : We investigated the expression of CD11b , CD 18 , CD 35 , CD 43 , CD 44 , and CD 69 on the peripheral blood monocytes , Cd11b , CD 18 , CD 35 , CD 43 , CD 44 , CD 67 on peripheral blood neutrophils and CD 38 and CD 69 on peripheral blood lymphocytes . ^^^ Results : The expression of CD11b , CD 18 , and CD 69 on the monocytes of group A was significantly greater than in groups B and C and in controls , while CD 44 was significantly downregulated in group A . ^^^ CD11b , CD 18 , CD 35 , CD 44 , and CD 67 on the surface of neutrophils and CD 38 and CD 69 on the surface of lymphocytes were also overexpressed in group A . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The HMO 6 cells exhibited cell type specific antigens for microglia macrophage lineage cells including CD11b ( Mac 1 ) , CD 68 , CD 86 ( B 7 2 ) , HLA ABC , HLA DR , and ricinus communis agglutinin lectin 1 ( RCA ) , and actively phagocytosed latex beads . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Using an array of function blocking monoclonal antibodies ( mAb ) ( anti CD11a , CD11b , CD 18 , CD49d , CD 54 , and CD 106 ) , we characterized the adhesive interactions underlying the transendothelial and transepithelial leukocyte traffic in intact animals . ^^^ Comigrating neutrophils were found to primarily utilize CD11b , CD 18 , and CD 54 , but not CD49d , CD 106 , or , surprisingly , CD11a . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Superoxide anion production by isolated peripheral blood granulocytes , their phagocytosis , and expression of cell adhesion molecules such as CD11a , CD11b , and CD 18 on granulocytes were measured by flow cytometry . ^^^ The levels of CD11a , CD11b and CD 18 expression on peripheral blood granulocytes and superoxide anion production by granulocytes were significantly suppressed by chemotherapy without CSF support . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In this study we analysed blood samples taken from the dorsalis pedis vein and a brachial vein of 11 healthy test persons and 8 patients with venous leg ulcer under experimental venous hypertension in order to examine changes in the expression of leukocyte adhesion molecules ( LFA 1 ( CD11a ) , Mac 1 ( CD11b ) , p 150 , 95 ( CD11c ) , CD 18 , VLA 4 ( CD49d ) and L selectin ( CD62L ) ) which are involved in the adhesion steps of leukocytes to endothelial cells for transmigration into tissues . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
VCAM 1 expression on endothelial cell and increased expression of CD11b CD 18 on monocytes may facilitate monocyte emigration and can represent one of the initial steps of vascular alteration . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
OBJECTIVE : To observe whether homocysteine can directly alter the expressions of CD11b , CD 18 , CD 14 and L selectin on neutrophils , monocytes , and lymphocytes in whole blood from healthy human subjects . ^^^ RESULTS : Homocysteine at the lowest concentration ( 20 mumol / L ) significantly increased surface adhesive molecule expressions of CD11b and CD 18 on each cell type and CD 14 on monocytes and neutrophils in whole blood . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In parallel to these results , the fMLP induced upregulation of CD11b ( Mac 1 ) levels on the PEG HbV pretreated PMNs was comparable to that of untreated cells . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Antibodies specific for epitopes of the alpha subunit ( CD11b ) and the beta ( 2 ) subunit ( CD 18 ) of Mac 1 were shown to completely eliminate lymphocyte adhesion to C . albicans hyphae . ^^^ In this study we identified macrophage 1 antigen ( Mac 1 ) ( CD11b / CD18 , alpha ( M ) / beta ( 2 ) ) as the lymphocyte surface structure responsible for the adhesion of activated lymphocytes to the hyphal form of the fungus . ^^^ Our results clearly identify Mac 1 ( CD11b / CD18 ) as the lymphocyte surface structure that mediates activated lymphocyte adhesion to C . albicans and the resultant antifungal effect of the lymphocytes . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
ANDRO pretreatment significantly decreased fMLP induced up expression of both CD11b and CD 18 . ^^^ We further examined the up expression of surface Mac 1 ( CD11b / CD18 ) , an essential integrin mediated in neutrophil adhesion and transmigration . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Following one week of diabetes , neutrophil CD11a , CD11b , and CD 18 expression was increased significantly , as were retinal ICAM 1 levels . ^^^ High dose aspirin also reduced the expression of CD11a , CD11b , and CD 18 , whereas meloxicam and etanercept did not . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Although neutrophil CD11a , CD11b , and CD 18 levels were increased in 1 week diabetic animals , VEGF TrapA ( 40 ) did not alter the expression of these integrin adhesion molecules . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
On the other hand , in t ( 8 ; 21 ) AML cells , expression of Ang 2 was infrequent and expression of integrin family adhesion molecules ( CD11b , CD11c and CD 18 ) was weak , suggesting the sparse association with endothelial cells . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
To determine whether or not granulocyte function is also impaired by chemotherapy , respiratory burst , CD11b and CD 18 expression were analysed by flow cytometry in granulocytes from 10 patients with haematological malignancies : before and after the commencement of high dose chemotherapy and in the recovery phase . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The Fes expressing differentiated cells became adherent , had distinctive macrophage morphology , and exhibited increased expression of myelomonocytic differentiation markers , including CD11b , CD11c , CD 18 , CD 14 , and the macrophage colony stimulating factor receptor . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
METHODS : Rats were assigned to one of six groups including sham operation , intestinal IR ( 60 / 120 min ) and IR plus treatment with one of the following monoclonal antibodies against CD11a , CD11b , CD 18 , and ICAM 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The effect of methanol washing of plasticized polyvinyl chloride on biomaterial contact mediated CD11b ( mac 1 ) expression in a rat recirculation model . ^^^ Our objective was to assess whether using a methanol wash to reduce the level of plasticizer present on the surface of medical grade polyvinyl chloride ( PVC ) has a moderating effect on the expression of CD11b ( mac 1 ) on neutrophils in rats undergoing recirculation . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The percentage of whole blood neutrophils indicating expression of selectins CD 18 , CD11b , integrin CD62L and mean fluorescence intensity ( MFI ) of these molecules were studied on flow cytometry . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Compared to healthy controls , there was decreased expression of CD11a , CD11b , CD 18 , CD49b , CD49c , CD49d , CD 54 , and CD 8 in patients ' lymphocyte populations and CD11a , CD 18 , CD49c , CD49d , and CD 8 in patients ' granulocyte populations . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Thus , in differentiating myeloid leukemia cells CD11b is predominantly expressed which can associate with CD 18 to form the functional heterodimeric integrin Mac 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Flow cytometry analysis revealed that LTD ( 4 ) significantly enhanced the expression of CD11b and CD 18 on the EOS surface . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We investigated effects of fosfomycin ( FOM ) on neutrophil function , specifically the oxidative burst and adhesion molecule expression ( CD11b / CD18 , or MAC 1 ) using flow cytometry assay . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In contrast , the expression of IL 8 receptor , CD11b , CD 18 and CD 62 was not significantly altered in HIV patients compared with healthy controls . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The expression of adhesion molecules ( CD11a , CD11b , CD 18 , CD49d , CD 54 , CD62L ) on peripheral blood mononuclear cells ( PBMCs ) of asthmatics and the expression of E and P selectins , ICAM 1 , VCAM 1 on cultured human umbilical vein endothelial cells ( HUVEC ) after stimulation with PBMCs from CDAs before and after 3 and 6 months of SeS were assessed by standard monoclonal antibodies and analyzed by flow cytometry . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Blocking monoclonal antibodies were used against the platelet and neutrophil surface receptors glycoprotein complex IIb / IIla , P selectin , ICAM 2 , CD11a , CD11b , and CD 18 . ^^^ The difference between the Pi of cells suspended in SPPP or NPPP ( deltaPi SPPP NPPP ) in the presence of monoclonal antibodies anti CD 41 , anti CD62P , abciximab , anti CD11a , anti CD11b , and anti CD 18 was significantly less than the APi SPPP NPPP of cell suspensions without the addition of these monoclonal antibodies ( P < 0 . 01 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The beta 2 integrins leukocyte function antigen 1 ( LFA 1 , CD11a ) and macrophage antigen 1 ( Mac 1 , CD11b ) have been reported to play a role in the attachment of CD 34 ( + ) cells to stromal cells in the bone marrow . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Resveratrol ( 10 microM ) enhanced the expression of adhesion molecules ( CD11a , CD11b , CD 18 , CD 54 ) in each of the cell lines except for Kasumi 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Fluorescence activated cell sorting ( FACS ) analysis of splenocytes from NF kappaB dKO mice revealed a two to threefold increase in the percentage of CD11b ( Mac 1 ) and RANK double positive cells compared with WT controls . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Human microglial cells exhibited cell type specific antigens for macrophage microglia lineage cells including CD11b ( Mac 1 ) , CD 68 , B 7 2 ( CD 86 ) , HLA ABC , HLA DR and ricinus communis aggulutinin lectin 1 ( RCA 1 ) , and actively phagocytosed latex beads . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
When monoclonal antibodies to mice VCAM 1 , CD11b or CD 18 were added separately to the co culture system , the number of TRAP positive cells that were generated from LFA 1 deficient mice was 20 30 % smaller than that generated from wild type mice . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
After EB challenge , levels of 10 of 17 markers ( CD11a , CD11b , CD 14 , CD 18 , CD 23 , CD 32 , CD 63 , CD 64 , HLA class 1 , and HLA DR ) were significantly increased ( by 33 234 % , P < 0 . 05 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The aim of this study was to evaluate the association of the expression of the adhesion molecules CD11a , CD11b , CD 18 , CD 54 , CD49d , and CD 62 L in white blood cells and levels of soluble adhesion molecules ICAM 1 and L selectin in serum with occupational exposure to styrene . ^^^ Expression of the adhesion receptor antigens CD11a on lymphocytes , CD11b on monocytes , and CD 18 on granulocytes were unaffected . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
After I / R , lack of enteral feeding increases organ expression of ICAM 1 , CD11b levels on myeloid cells , and lung of CD 18 positive leukocytes . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Lkt binds to beta ( 2 ) integrins on the surface of bovine leukocytes . beta ( 2 ) integrins have a common beta subunit , CD 18 , that associates with three distinct alpha chains , CD11a , CD11b , and CD11c , to give rise to three different beta ( 2 ) integrins , CD11a / CD18 ( LFA 1 ) , CD11b / CD18 ( Mac 1 ) , and CD11c / CD18 ( CR 4 ) , respectively . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
No concentration of cetirizine or levocetirizine had any significant effect on expression of CD11b , CD 18 or CD49d by either resting or eotaxin stimulated eosinophils . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Peripheral blood was collected at the day prior to surgery and the morning of postoperative days 1 , 2 , 5 , and 7 for determination of the expression of leukocyte CD11a , CD 18 , CD11a / CD18 , CD11b , CD11b / CD18 ( immunofluorescent flow cytometry ) and the plasma soluble intercellular adhesion molecule 1 ( sICAM 1 ) , soluble E selectin ( sE selectin ) ( enzyme linked immunoabsorbent assay ) . ^^^ CPB caused a sustained increase in the leukocyte CD11a , CD 18 , CD11a / CD18 , CD11b / CD18 expression and the plasma sICAM 1 , sE selectin levels in patients ( P < 0 . 05 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Expression of the adhesion molecules CD11b and CD 18 increased on neutrophils in milk at 24 h then tended to decline over subsequent days . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In addition , TNF alpha induced GPI 80 release was inhibited by blocking monoclonal antibodies specific to components of Mac 1 ( CD11b and CD 18 ) . 4 Antioxidants ( pyrrolidine dithiocarbamate and N acetyl L cysteine ) inhibited GPI 80 release by TNF alpha stimulation , but superoxide dismutase did not . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The PMN adhesion molecules ( CD11a , CD11b , CD11c , CD 18 ) were evaluated using indirect immunofluorescence and a flow cytometer , at baseline and after in vitro activation with 4 phorbol 12 myristate 13 acetate ( PMA ) and N formyl methionyl leucyl phenyl alanine ( fMLP ) . ^^^ At baseline , in diabetic subjects the phenotypical expression of CD11a and CD11b was significantly reduced and CD11c was increased , whereas CD 18 was unchanged in comparison with normals . ^^^ Considering type 1 and 2 diabetic subjects separately , CD11a was reduced and CD11c was increased in both subgroups , CD11b was decreased only in type 1 diabetics and CD 18 , decreased in type 1 , was increased in type 2 subjects . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Promotion of apoptosis by iIgA was almost completely prevented by blocking antibodies directed to CD 18 or CD11b and was shown to be dependent on the activation of the respiratory burst as suggested by the ability of catalase to prevent apoptosis stimulation ; the effect of azide , an heme enzyme inhibitor that significantly increased promotion of apoptosis by iIgA ; and the inability of iIgA to stimulate apoptosis of neutrophils isolated from chronic granulomatous disease patients . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Maternal blood was assayed to determine granulocyte and monocyte phenotype using monoclonal antibodies , which included cluster differentiation ( CD ) markers CD11b , CD 14 , CD 15 , CD 16 , CD 18 , CD49d , CD62L , CD 64 , CD66b and human leukocyte antigen ( HLA ) DR . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Activation of the CD40 / CD40L dyad with anti CD 40 antibody produced a small release of arachidonic acid and lacked any significant effect on the induction of cyclooxygenase 2 , whereas the secretion of the chemokine MCP 1 and the surface display of CD11b , the alpha chain of the integrin Mac 1 , were upregulated . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
A gene delivery system using bone marrow derived CD11b ( + ) CD 18 ( + ) cells and their interaction with adhesion molecules was established . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We measured interleukin ( IL ) 1beta , tumour necrosis factor ( TNF ) alpha , IL 8 , IL 10 , IL 1ra and TNF sr 2 levels , and the expression of HLA DR and the adhesion molecules L selectin , CD 18 and CD11b on monocytes , granulocytes and lymphocytes . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
No significant changes could be found in the cell surface expression of CD 80 , CD 86 , MHC 1 , MHC 2 , CD 18 , CD11b , CD 40 , CD 25 , and CD 54 in antigen presenting cells ( APC ) from control or DSG treated animals . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
When adherence was disrupted by pipetting , by a combination of Abs specific for CD11b , CD 18 , and CD 54 , or by an antisense oligonucleotide targeting CD 18 mRNA , both cell apoptosis and bacterial killing induced by IFN gamma were inhibited . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
To investigate the function of the main adhesion receptors ( CD62L , CD49d , CD49e , CD11b and CD 18 ) on CD34+ cells during homing , their expression was quantified by flow cytometry using calibration beads . ^^^ The beta _ 2 integrins ( CD11b and CD 18 ) receptors are also implicated in CD34+ cells homing to BM . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In addition , the expression of CD11b and CD 18 on PMN and PMN dependent adhesion to endothelial cells elicited by HPE was inhibited by polaprezinc and zinc sulphate in a concentration dependent manner . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Cell surface expression of CD 18 , CD11a , CD11b , CD11c , CD 50 , and CD 54 was not affected by Na ( 2 ) SO ( 3 ) treatment . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Recombinant hsp 60 and Abs to CD11b and CD 18 inhibited binding of yeasts to Chinese hamster ovary cells transfected with CR 3 ( CHO 3 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Functional inhibition by antibodies suggested that Mac 1 ( CD11b ) integrin plays a central role in complement 5a induced migration . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Differences in expression of the neutrophil adherence molecules , L selectin and CD11b / CD18 ( Mac 1 ) , have been previously demonstrated in cord blood and in very young infants ( < / = 48 h ) compared with adults , but it is unknown for how long these differences persist . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Adhesion of dendritic cells derived from CD34+ progenitors to resting human dermal microvascular endothelial cells is down regulated upon maturation and partially depends on CD11a CD 18 , CD11b CD 18 and CD 36 . ^^^ Mature DC lacked expression of CD 31 , CD 36 , CD45RA and CLA , and expressed lower levels of CD11a , CD11b and CD49d as compared with precursors tested on day 5 . mAb against CD 18 , CD11a , CD11b , and CD 36 markedly inhibited DC binding , whereas anti CLA , anti DC SIGN , anti CD 29 and anti CD 49 mAb did not . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The broad reactivity of the antibody together with its capture by CD11b and CD 18 in monoclonal antibody immobilization of granulocyte antigen test suggested HNA 4a specificity . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
IL 3 , IL 5 , and GM CSF could enhance p 38 MAPK and NF kappaB activity and induce ICAM 1 , CD11b , and CD 18 expressions on eosinophils . ^^^ Only SB 203580 could reverse the effect on CD11b , CD 18 , and ICAM 3 expressions . ^^^ Therefore , the expression of ICAM 1 might involve both p 38 MAPK and NF kappaB activities , whereas the regulation of CD11b , CD 18 , and ICAM 3 expressions might be mediated through p 38 MAPK but not NF kappaB . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The cells were exposed to 1 nm FMLP , 5 pm free ICAM 1 , or antibodies binding ICAM 1 or the beta 2 integrins CD11a , CD11b , CD11c and CD 18 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
As evidenced by flow cytometry , ATRA ( at 1 microM ) clearly and donor independently suppressed the expression of all integrin chains investigated ( CD11a , CD11b , CD11c , and CD 18 ) , most strikingly of CD11a . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Hepatic killing but not clearance of systemically circulating bacteria is dependent upon peripheral leukocytes via Mac 1 ( CD11b / CD18 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
BACKGROUND : Increased expression of the beta 2 integrin Mac 1 ( CD11b / CD18 , alphaMbeta 2 ) , which is responsible for firm leukocyte adhesion to platelets and fibrinogen at injured vessels , is found in association with neointimal hyperplasia after coronary interventions . ^^^ METHODS AND RESULTS : Expression of CD11b ( alpha subunit of Mac 1 ) and binding of 8B2 ( monoclonal antibody against an activation dependent neoepitope of Mac 1 ) on the surface of polymorphonuclear leukocytes were analyzed in 62 patients undergoing coronary stenting using flow cytometric analysis of whole blood obtained from the coronary sinus and femoral vein . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We evaluated the expression of some PMN integrins ( CD11a , CD11b , CD11c , CD 18 ) , using flow cytofluorimetry , at baseline and after in vitro activation with 4 phorbol 12 myristate 13 acetate . ^^^ Type 2 diabetic subjects with MVC showed , compared to normals , an increase of CD11a and CD 18 and a decrease of CD11b and CD11c . ^^^ In type 2 diabetic patients with MVC the basal upregulation of CD11a and CD 18 may be related to the PMN spontaneous activation , while the behavior of CD11b may depend on its self consumption . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
This was documented by the expression of integrin Mac 1 molecule ( CD11b ) on the cell surface and the cellular adhesion to the plastic bottom of the flask , indicating the differentiation along with the monocyte / macrophage lineage . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Whole blood samples were collected before shock as well as at 1 and 2 h after the end of the shock period for neutrophil CD11b and CD 18 expression measurements . ^^^ In a second set of experiments , mesenteric lymph samples collected from rats subjected to trauma plus hemorrhagic shock ( T / HS ) or trauma plus sham shock ( T / SS ) and resuscitated with Ringer ' s lactate or hypertonic saline were tested for their ability to modulate PMN CD11b , CD 18 , or L selectin expression , as well as prime PMN for an augmented respiratory burst . ^^^ Both CD11b and CD 18 expression were increased in PMN harvested from rats subjected to T / HS and resuscitated with Ringer ' s lactate solution , but not in T / HS rats resuscitated with hypertonic saline . ^^^ Likewise , mesenteric lymph from the T / HS rats resuscitated with Ringer ' s lactate increased naive rat and human PMN CD11b and CD 18 expression to a greater extent than did T / HS lymph from the hypertonic saline treated rats . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The neutrophils and natural killer cells that are mobilised during exercise also express high levels of Mac 1 ( CD11b ) , a marker associated with cellular activation . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Functional alterations in CD11b ( + ) Gr 1 ( + ) cells in mice injected with allogeneic tumor cells and treated with 2 , 3 , 7 , 8 tetrachlorodibenzo p dioxin . 2 , 3 , 7 , 8 Tetrachlorodibenzo p dioxin ( TCDD ) exposure results in an increased percentage of CD11b ( + ) ( Mac 1 ( + ) ) cells in the spleens of mice challenged with P 815 tumor cells , coincident with a failure of the mice to generate allospecific CD 8 ( + ) CTL activity . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Using the pre procedural expression levels , patients with restenosis could be predicted by discriminant analysis with CD11a , CD11b , and CD 18 ( average recognition index = 95 . 5 % ) . ^^^ Moreover , CD11a , CD11b , and CD 18 may be helpful as indicators for the progression of CAD . . ^^^ Surface expression of leukocyte adhesion molecules Mac 1 ( CD11b / CD18 ) , LFA 1 ( CD11a / CD18 ) , L Selectin ( CD62L ) , ICAM 1 ( CD 54 ) , and MHC 2 ( HLA DR ) were assessed by flow cytometry . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In animals that received MPTP injection , an increase in immunostaining of GFAP , glutamine synthetase , and Mac 1 ( CD11b ) in astrocytes , Mller cells , and microglia was invariably observed , indicating an activation or dysfunction of retinal glial cells . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Moreover , induction of IP 10 was mediated via specific adhesion molecules , as indicated by the finding that both anti integrin ( CD11b and CD 18 ) and intercellular adhesion molecule 1 antibodies significantly inhibited IP 10 induction . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
METHODS : Trace cytotoxicity test plates were utilized in the detection of CD 3 , CD 4 , CD 8 , CD11a , CD11b , CD 18 and CD 54 on lymphocytes by APAAP immunocytochemical staining . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Expression of CD11b , CD 18 , CD 45 , CD45RA , CD45RB , CD45RO , CD 69 , CD 95 , IL 5Ralpha , IL 9Ralpha , CCR 1 , CCR 3 , and CXCR 4 by CE as they matured in culture were assessed by immunostaining and flow cytometry and expression of these receptors compared with freshly isolated PBE . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Thirty three genes were found constantly differentially expressed , among which were cd11b , cd 18 , cd 68 , osteopontin and ferritin heavy chain upregulated in AL and collagen types 1alpha 1 , 3alpha 1 , integrin alpha 1 , thrombospondin 2 and nidogen upregulated in SL . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Analysis with Kruskal Wallis test showed no statistical significant differences in the expression of CD 54 , CD11a , CD11b and CD 18 in three groups examined . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibody blocking studies showed that the combination of mAb to CD11b and CD 18 reduced lytic activity against both PAEC as well as K 562 , although other qualitative differences were observed between the porcine and human target cells . ^^^ These findings suggest that the oxidative stress induced downregulation of CD 18 may be important in modulating cytotoxic activity of fresh NK cells against PAEC and K 562 targets through reduced formation of the CD11b / CD18 heterodimer . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Cytokines interleukin 8 ( IL 8 ) , interleukin 10 ( IL 10 ) , interleukin 1 receptor antagonist ( IL 1ra ) and tumour necrosis factor soluble receptor 2 ( TNFsr 2 ) and the leucocyte adhesion molecules L selectin , HLA DR , CD 18 and CD11b were determined . ^^^ Increases in granulocyte CD11b and CD 18 expression were less in the methylprednisolone group than in the control group but did not reach statistical significance . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Following an additional 3 days of culture in the presence of GM CSF , IL 4 , IL 1beta , IL 6 , TNFalpha , and PGE ( 2 ) , cells ( 82 . 0 + / 5 . 8 % CD83+ ) displayed a mature DC morphology and phenotype , including expression of CD11b , CD11c , CD 18 , CD 25 , CD 40 , CD 54 , CD 58 , CD 80 , CD 86 , HLA class 1 , and HLA DR as well as expression of CCR 7 but not CCR 5 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
To test our hypothesis , we perturbed the extracellular , membrane proximal regions of individual CD11b and CD 18 subunits and studied their effect on ligand binding , receptor clustering , and lipid raft association . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In contrast , in both the baboon and human studies , other neutrophil surface antigens were only slightly decreased ( CD11a ) or increased ( CD11b , CD 18 , CD 35 , CD66b , and CD 63 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Flow cytometric analyses revealed an upregulation of the function associated molecules Fc gamma receptor 3 ( Fc gamma R 3 ) and 2 ( CD 16 and CD 32 ) as well as MAC 1 ( CD11b and CD 18 ) on BCG stimulated PMN . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In the present study , we have investigated the effects of blocking monoclonal antibodies ( mAbs ) directed against CD49d ( alpha ( 4 ) integrin ) , CD 18 ( beta ( 2 ) integrin ) and the alpha sub units of beta ( 2 ) integrin CD11a ( LFA 1 integrin ) and CD11b ( Mac 1 integrin ) , on antigen ( Ag ) induced acute bronchoconstriction and cellular recruitment in allergic rabbits in vivo . ^^^ However in contrast , lymphocyte recruitment appears to be mediated by a combination of LFA 1 ( CD18 / CD11a ) and Mac 1 ( CD18 / CD11b ) . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Compared with PMN , monocytes exhibited an expression level that was similar for CD 18 , higher for CD11a and CD11c , but lower for CD11b . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Exposure of 1 10 10 ( 6 ) cells / ml of KG1a cells to 100 ng / ml PF 4 the increased total adherence of KG1a cells was mediated by PECAM 1 ( CD 31 ) , CD 44 , LFA 1 ( CD11a ) and Mac 1 ( CD11b ) but not by P selectin ( CD62P ) and E selectin ( CD62E ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The aim of this in vivo study was to examine the effect of intramammarily administered endotoxin ( lipopolysaccharide , LPS ) on the expression of L selectin ( CD62L ) and the beta 2 integrin subunits CD11b and CD 18 on circulating bovine PMN . ^^^ In analogy with observations during clinical coliform mastitis , a progressive decrease of CD62L expression levels was observed early after LPS infusion , concomitantly with a continuous rise of CD11b and CD 18 density . ^^^ However , no correlation was found between the kinetics of CD11b and CD 18 density . ^^^ Interestingly , in contrast to coliform mastitis , the net CD 18 variation is not principally influenced by CD11b upregulation during endotoxin administration . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Repeatedly TCDD treated mice contained more CD11b ( Mac 1 ) positive cells in the spleen and higher titers of tissue specific autoantibodies than the vehicle treated group . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Fcgamma receptors induce Mac 1 ( CD11b / CD18 ) mobilization and accumulation in the phagocytic cup for optimal phagocytosis . ^^^ Functional interactions between Fcgamma receptors ( FcgammaR ) and the beta 2 integrin Mac 1 ( CD11b / CD18 ) have been described , but the molecular basis of this relationship remains unclear . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Soluble intercellular adhesion molecule 1 and soluble vascular cell adhesion molecule 1 were measured by enzyme linked immunosorbent assay and expression of the monocyte integrins CD11b ( Mac 1 ) and CD49d ( VLA 4 ) was measured by direct immunofluorescence using flow cytometry . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In each case , this was accompanied by a significant reduction in the percentage of cells expressing the differentiation markers CD11a , CD11b , and CD 18 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The purpose of this study was to characterize the effects of orbofiban on cellular activation ( neutrophil superoxide generation ) and surface expression of adhesion molecules of circulating neutrophils ( CD 18 , CD11b , and L selectin ) and platelets ( P selectin and GP IIb / IIIa ) in patients with acute coronary syndromes . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Previous studies using blocking monoclonal antibodies suggested roles for CD 18 and CD11b in hepatic neutrophil recruitment and host innate response to Listeria monocytogenes , a gram positive intracellular bacterium . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
At 1800 s ( 1 ) , shear and agonist induced PLA formation were maintained or further enhanced with GPIIb / IIIa blockade alone , but reduced by simultaneous blockade of GPIIb / IIIa , CD11b , and CD 18 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The activation of WBCs was determined by measuring adhesion molecule CD11b and CD 18 expression on the surface of granulocytes and monocytes . ^^^ Baseline expression of CD11b or CD 18 on monocytes did not change significantly during the study period . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
OBJECTIVE : The aim of this study was to investigate the expression of intercellular adhesion molecule 1 ( ICAM 1or CD 54 ) and the alpha subunits of its ligands ' lymphocyte function associated antigen 1 ( LFA 1 ) ( alphaL subunit or CD11a ) and Mac 1 ( alphaM subunit or CD11b ) on peripheral blood leucocytes in infants with cow ' s milk allergy ( CMA ) and in healthy controls . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
METHODS : At 1 and 4 hours after hemorrhagic shock ( 30 mm Hg 10 90 minutes ) plus trauma ( laparotomy ) ( T / HS ) or sham shock ( T / SS ) , with or without mesenteric lymph duct ligation , PMN CD11b and CD 18 expression was assessed in male rats . ^^^ RESULTS : Compared with T / SS rats , T / HS was associated with up regulation of PMN CD11b and CD 18 expression , which was largely prevented by ligation of the mesenteric lymph duct ( p < 0 . 01 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In addition , it also significantly inhibited both monocyte endothelium adhesion and transendothelial monocyte migration , although monocyte surface expression of relevant adhesive molecules ( CD11b , CD 18 , CD 49 days , CCR 2 ) was unchanged . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
GBS induced TNF alpha release was inhibited by monoclonal antibodies directed against each of the components of CR 3 and CR 4 : the common integrin beta subunit CD 18 and the alpha subunits CD11b ( of CR 3 ) and CD11c ( of CR 4 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Co stimulating CD 18 and CD11b with activating antibodies resulted in NF kappaB activation by GM CSF and IL 8 in suspended cells . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
That increase was independent of IFN gamma production by T cells , but was mediated by integrins CD11b , CD 18 , and CD 29 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
AIMS : To study a longitudinal change in the expression of adhesion molecules CD11b , CD 18 , and CD62L on neutrophils and monocytes in very low birth weight babies who develop respiratory distress syndrome , to compare these levels between bronchopulmonary dysplasia ( BPD ) and non BPD infants , and to assess the effect of corticosteroid treatment on these adhesion molecules . ^^^ Expression of CD11b , CD 18 , and CD62L was measured on neutrophils and monocytes in arterial blood on days 1 , 3 , 7 , 14 , 21 , and 28 , and before and 2 3 days after initiation of dexamethasone treatment by flow cytometry . ^^^ Dexamethasone treatment significantly decreased CD11b , CD 18 , and CD62L expression on neutrophils , and CD11b and CD18L expression on monocytes . ^^^ Dexamethasone use was associated with decreased expression of CD11b , CD 18 , and CD62L . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
PROCEDURES : Neutrophil size and granularity ; expression of cell surface molecules including CD 18 , CD11b , and mature neutrophil antigen on neutrophils ; and major histocompatability antigen class 2 ( MHC class 2 ) expression on monocytes and lymphocytes were evaluated by use of flow cytometry . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Eotaxin was mainly expressed in subepidermal tissue in close proximity to eosinophils that expressed high levels of adhesion molecules ( CD11b , CD11c , CD 18 and CD49d ) and the activation marker HLA DR . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
A did not label intracellular stores as did both cross reacting anti canine CD11b and CD 18 , suggesting that an activation induced conformational change would expose a neoepitope recognized by F21 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In 27 subjects with VAD we examined , at baseline and after in vitro activation with 4 phorbol 12 myristate 13 acetate ( PMA ) , the PMN integrin pattern ( CD11a , CD11b , CD11c , CD 18 ) using indirect immunofluorescence and a flow cytometer . ^^^ At baseline VAD subjects showed an increase of CD11a and CD 18 and a decrease of Cd11b and Cd11c as compared to normal subjects . ^^^ After activation , in normal subjects , we found an increase in the expression of all integrins , while in VAD subjects we observed an increase of CD11b and Cd11c and a decrease of Cd11a and CD 18 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The following phenotypic and functional parameters of the monocyte population were studied by flow cytometry : surface molecules expression ( CD 18 , CD11a , CD11b , CD 14 , CD 15 , CD 45 , HLA DR , and Toll like receptor [ TLR ] 4 ) , myeloperoxidase ( MPO ) content , and intracellular cytokine production ( tumor necrosis factor [ TNF ] alpha , interleukin [ IL ] 1beta , IL 6 , and IL 8 ) . ^^^ RESULTS : Cardiac surgery with CPB induced down modulation of surface molecules expression on peripheral monocytes , especially at 24 h after CPB , for CD 18 , CD11a , and CD11b ( P < 0 . 003 ) and for the CD 15 adhesive cluster ( P = 0 . 0028 ) and HLA DR ( P < 0 . 001 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
DESIGN : This study utilized quantitative flow cytometry to determine whether the expression of surface adhesion molecules : CD 18 , CD11a , CD11b , and CD11c on cord blood neutrophils using mean channel fluorescence values ( MCF ) . ^^^ RESULTS : MCF values were significantly higher in infants born to pre eclamptic women vs controls : CD 18 ( 432 . 0+ / 236 . 3 vs 230 . 5+ / 97 . 9 ; p=0 . 002 ) , CD11a ( 552 . 9+ / 272 . 4 vs 326 . 9+ / 268 . 6 ; p=0 . 003 ) , CD11b ( 937 . 2+ / 521 . 9 vs 576 . 6+ / 352 . 9 ; p=0 . 025 ) , and CD11c ( 228 . 5+ / 130 . 3 vs 133 . 0+ / 77 . 1 ; p=0 . 006 ) , respectively . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies directed against CD 18 , CD11a , CD11b , CD11c , CD 21 , CD 3 , CD 4 , CD 8 and Major Histocompatibility Complex class 1 and 2 ( MHC 1 and MHC 2 ) were also employed . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Expression of adhesion receptors CD11a , CD11b , CD 18 , CD49d , and CD 54 on blood and peritoneal leukocytes was measured using flow cytometry . ^^^ RESULTS : In macrophages from the uninfected effluents , expression of both subunits of Mac 1 integrin receptor ( CD11b and CD 18 ) and intercellular adhesion molecule ( ICAM ) 1 receptor ( CD 54 ) was upregulated compared to peripheral blood monocytes from the same patients . ^^^ The median value of mean fluorescence intensity in blood and effluent was 760 . 3 versus 1085 . 8 for CD11b ( p = 0 . 013 ) , 288 . 8 versus 448 . 6 for CD 18 ( p = 0 . 003 ) , and 186 . 1 versus 365 . 7 for CD 54 ( p = 0 . 001 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Concomitantly , Mabs against CD 63 and CD 82 diminished the surface expression of CD 29 , CD11b , CD 18 , and alpha 5 integrins . ^^^ By immunoprecipitation experiments we found that CD 63 associated with integrins CD11b and CD 18 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The expression of adhesion molecules CD11a , CD11b , and CD 18 on granulocytes decreased directly after the LCP treatment . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The defect in T cell proliferation with SEA was more severe in splenocytes from mice deficient in CD 18 , CD11b , or CD11d than in CD11a deficient splenocytes , with a normal response in CD11c deficient splenocytes . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Monocytes of type 1 diabetic patients displayed an increased adhesion to fibronectin in comparison with type 2 patients and healthy control subjects but had a normal expression of the FN binding integrins CD 29 , CD49a , CD49d , and CD49e ( although CD11b and CD 18 expression was increased ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
When beta 2 ( CD 18 ) integrin or lymphocyte function antigen ( LFA ) 1 ( CD11a ) plus Mac 1 ( CD11b ) were blocked by mAb ( intact or Fab ' ) , the proportion of migrated PMN ( but not of non migrated PMN ) with active caspases was significantly increased ( 2 4 fold ) and this was associated with accelerated PMN apoptosis and death . ^^^ After 20 h , caspase activation ( Western blotting ) was markedly decreased in PMN cultured on fibrinogen , a ligand for Mac 1 ( CD11b / CD18 ) , but not on fibronectin or albumin . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
OBJECTIVE : To evaluate L selectin ( CD62L ) and Mac 1 ( CD11b ) expression at the surface of blood and milk neutrophils during the early inflammatory response to Escherichia coli induced mastitis in cows . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Surface expression of Mac 1 ( CD11b , CR 3 ) on PMN and of CD 14 on monocytes was measured in early ( EL ) , peak ( PL ) , and midlactation ( ML ) by flow cytometric analysis . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Both components of LFA 1 , CD11a and CD 18 , were downregulated during treatment , and surprisingly the integrins CD11b and beta 7 were similarly reduced . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
AIM : To study how intestinal lymph after trauma induced shock ( TIS ) interferes with expression of neutrophil adhesion factors ( CD11b and CD 18 ) and causes lung injury . ^^^ Expression of neutrophil CD 18 and CD11b in at 1 and 3 h after a 90 min TIS / sham TIS was evaluated . ^^^ RESULTS : The expressions of CD 18 and CD11b at 1 and 3 h after a 90 min TIS and the main lung injury indexes were significantly increased compared with those in the sham TIS groups ( P < 0 . 05 ) . ^^^ Moreover , at 1 and 3 h after TIS , the expressions of CD 18 ( 32 . 12+ / 1 . 25 and 33 . 46+ / 0 . 98 ) and CD11b ( 29 . 56+ / 1 . 35 and 30 . 56+ / 1 . 85 ) were significantly decreased in rats with ligation of mesenteric lymph duct , compared with those ( 52 . 3+ / 1 . 12 and 50 . 21+ / 1 . 25 , and 42 . 24+ / 1 . 24 and 42 . 81+ / 1 . 12 , respectively ) in those without the ligation ( all P < 0 . 05 ) . ^^^ However , there was no significant difference in expressions of CD 18 and CD11b and the main lung injury indexes between the two sham TIS groups . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
A stent induced increase in platelet P selectin ( CD62P ) expression and an increase in neutrophil Mac 1 ( CD11b ) expression were suppressed in the cilostazol group compared with the ticlopidine group . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Inflammatory reactions in the wear particle induced and infectious periprosthetic membrane of loosened hip and knee endoprostheses : pathogenetic relevance of differentially expressed genes cd 9 , cd11b , cd 18 , cd 52 and pdgfrbeta ] . ^^^ RESULTS : RT PCR showed the genes cd 9 , cd11b , cd 18 , cd 52 as well as pdgfrbeta in interface membranes . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The aim of this study was to investigate levels of CD 14 and the beta ( 2 ) integrin subunits CD11b and CD 18 on monocytes from women with diabetes or impaired glucose tolerance . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In the present study , the intracellular trafficking of Mac 1 was visualized directly by monitoring the fluorescent intensities of YFP CD 18 and PE conjugated monoclonal antibody against CD11b under a confocal microscope in CHO Mac 1 FP cells . ^^^ The fluorescence of CD 18 and CD11b reappeared on the cell membrane 1 h after re treatment with PMA , suggesting the recycling of non degraded Mac 1 . ( 2 ) The adhesion rate of CHO Mac 1 FP to magnetic beads coupled ICAM 1 increased within 4 h after their initial interaction , accompanied by the clustering of Mac 1 FP . ^^^ Previously , we constructed DNA vectors containing cDNA of Mac 1 subunits ( CD11b or CD18b ) fused with fluorescence protein ( FP ) . cDNA fragments and the DNA constructs were then transfected into CHO cells ( as CHO Mac 1 FP ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Each person was evaluated for inflammatory markers , including white blood cell counts , cell bound ( CD11b , CD 18 , CD 31 , CD62L ) and circulating soluble adhesion molecule levels ( sICAM 1 , sPECAM 1 , sL , sE , and sP selectins ) , serum CRP ( C reactive protein ) , fibrinogen , and interleukin 6 ( IL 6 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In a group of patients with mild CRF we determined , at baseline and after in vitro activation with 4 phorbol 12 myristate 13 acetate ( PMA ) and N formyl methionyl leucyl phenylalanine ( fMLP ) , the polymorphonuclear leukocytes ( PMN ) beta 2 integrin pattern ( CD11a , CD11b , CD11c and CD 18 ) by using indirect immunofluorescence with a flow cytometer . ^^^ At baseline we observed an increase in the phenotypical expression of CD11b , CD11c and CD 18 in CRF patients . ^^^ In normal subjects , after activation with both agents , we noted an increase of all adhesion molecules , while in CRF patients we found an increase in the expression of CD11b , CD11c and CD 18 but not of CD11a . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
METHODS : Expression of L selectin , CD 18 , and CD11b and leukocyte adhesion to activated endothelium from human umbilical veins were measured in 25 patients undergoing major orthopedic surgery . ^^^ In comparison with venous blood , CD11b was up regulated in SWB 1 . 3 to 3 . 6 fold on monocytes and neutrophils , whereas L selectin and CD 18 decreased on monocytes by 53 % and 15 % , respectively ( P < 0 . 05 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
To test this hypothesis , we studied neutrophil and monocyte surface densities of CD11b and CD 18 in patients undergoing elective infrarenal abdominal aortic aneurysm repair . ^^^ Serial blood samples were collected from the radial artery and femoral vein during the operation and leukocyte CD11b and CD 18 surface densities were quantified by flow cytometry . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
RESULTS : I / R plasma ( withdrawn 15 min following tourniquet release ) increased isolated neutrophil CD11b ( P = 0 . 03 ) , CD 18 ( P = 0 . 01 ) and endothelial intercellular adhesion molecule 1 ( ICAM 1 ) ( P = 0 . 008 ) expression compared to baseline . ^^^ CD11b , CD 18 and ICAM 1 expression on lidocaine ( 0 . 005 mg mL ( 1 ) ) treated neutrophils was similar to control . ^^^ CD11b ( P < 0 . 001 ) , CD 18 ( P = 0 . 03 ) and ICAM 1 ( P = 0 . 002 ) expression on lidocaine ( 0 . 05 mg mL ( 1 ) ) treated neutrophils and HUVECs was less than that on controls . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
METHODS : Isolated human neutrophil CD11b and CD 18 , and human umbilical vein endothelial cell ( HUVEC ) ICAM 1 expression and supernatant IL 1beta concentrations in response to hypoxia reoxygenation were studied in the presence or absence of different concentrations of lidocaine ( 0 . 005 , 0 . 05 and 0 . 5 mg mL ( 1 ) ) . ^^^ RESULTS : Exposure to hypoxia reoxygenation increased neutrophil CD11b ( 94 . 33+ / 40 . 65 vs . 34 . 32+ / 6 . 83 mean channel fluorescence ( MCF ) , P = 0 . 02 ) , CD 18 ( 109 . 84+ / 35 . 44 vs . 59 . 05+ / 6 . 71 MCF , P = 0 . 03 ) and endothelial ICAM 1 ( 146 . 62+ / 16 . 78 vs . 47 . 29+ / 9 . 85 MCF , P < 0 . 001 ) expression compared to normoxia . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Cross linking of the Mac 1 molecule ( CD 18 + CD11b ) was shown to result in rapid cell surface expression of CD 80 , CD 86 and DR antigen on the surface of normal human peripheral blood neutrophils . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
PMNLs incubated with culture supernatant ( CS ) from untreated or from tumour necrosis factor alpha ( TNF alpha ) treated ( 1 hr , 100 U / ml , 37 degrees ) monocyte derived DCs ( moDCs ) had increased surface expression of both CD11b and CD 18 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Fluorescent in situ hybridization analysis , multicolor fluorescence activated cell sorting , and quantitative reverse transcription PCR revealed that MVMp was expressed in rare subpopulations of CD11b ( Mac 1 ) positive cells displaying CD11c+ ( myeloid dendritic cells [ MDC ] ) or CD45B ( B220+ [ B 1 lymphocytes ] ) markers . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
METHODS : We studied changes in adhesion molecule expression of platelets ( GPIIb / IIIa ) , neutrophils CD 18 , CD11b and L selectin and monocytes ( CD 14 ) after interaction with active lesions of patients with stable angina subjected to PTCA and patients with unstable angina ( UA ) . ^^^ RESULTS : PTCA induced a marked up regulation of CD 18 , CD11b , CD 14 and GPIIb / IIIa with L selectin shedding and reduced SO formation , whereas only minor L selectin down regulation and decreased SO production indicated activation in UA . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The following parameters were studied : surface molecules expression ( CD 18 , CD11b , CD 44 , CD 162 , CD15s , CD 80 , CD 86 , CD 16 , CD49d , CD 29 , CD 25 , HLA DR , Toll like receptor 4 [ TLR 4 ] , CXCR 1 , CCR 2 , CCR 5 , CX3CR1 ) , oxidative burst response , monocyte platelet conjugates ( using antibodies against CD 45 , CD 14 , CD41a ) , and platelet activation ( CD62P , PAC 1 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Flow cytometric analyses demonstrated that IL 8 ( 10 ng / ml ) significantly augments CD11b ( Mac 1 integrin subunit ) expression on SCA neutrophils , but not normal neutrophils . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Here , we show that treating neutrophils with intact monoclonal antibody ( mAb ) to CD 18 activates the cells to express more CD11b . ^^^ Neutrophil differentiated HL 60 cells model Mac 1 ( CD11b / CD18 ) independent neutrophil transepithelial migration . ^^^ Previously , using function blocking monoclonal antibodies , we showed that neutrophil migration across intestinal epithelial monolayers in response to various chemoattractants was partially beta ( 2 ) integrin Mac 1 ( CD11b / CD18 ) independent . ^^^ We conclude that Mac 1 independent transepithelial migration does not require prior activation of cells via Mac 1 ligation because HL 60 cells lacking Mac 1 ( CD11b / CD18 ) expression migrate effectively . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
To inactivate chronically the beta 2 integrin CD11b ( Mac 1 ) , we made a transgenic model in mice in which we expressed the CD11b antagonist polypeptide neutrophil inhibitory factor ( NIF ) . ^^^ To validate the effectiveness of CD11b blockade , we compared PMN function in NIF ( + / + ) and Mac 1 deficient ( Mac 1 ( / ) ) mice . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The beta 2 integrins are found exclusively in leukocytes and they are composed of a common beta chain , CD 18 , and one of four unique alpha chains , CD11a ( alphaL subunit ) , CD11b ( alphaM subunit ) , CD11c ( alphaX subunit ) , or CD11d ( alphaD subunit ) . alphaX beta 2 which binds several ligands including fibrinogen and iC3b is expressed in monocytes / macrophages and dendritic cells playing an important role in the host defense . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
RANTES and eotaxin enhance CD11b and CD 18 expression on eosinophils from allergic patients with eosinophilia in the application of whole Blood flow cytometry analysis . ^^^ After hemolyzation with lysing solution , expression of CD11b , CD11a , CD 18 and CD49d on eosinophils was measured using flow cytometry . ^^^ RESULTS : The expression of CD11b , CD11a and CD 18 in allergic patients was significantly higher than that in normal subjects . ^^^ CD11b and CD 18 expression showed a significant increase after stimulation with C C chemokines , which was remarkable in allergic patients . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
This immortalized microglia HMO 6 cell line exhibits cell type specific antigens for microglia , including CD11b ( Mac 1 ) , CD 68 , CD 86 ( B 7 2 ) , HLA ABC , HLA DR , and RCA 1 lectin , and actively phagocytoses latex beads . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Neutrophils ( PMNs ) , isolated from healthy volunteers , were exposed to the FH supernatant and activation was examined ( CD11b and CD 18 adhesion receptor expression and respiratory burst ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Neutrophils were treated with IL 15 and cell surface expression of CD11a , CD11b , CD11c and CD 18 was monitored by flow cytometry . ^^^ IL 15 significantly increased neutrophil cell surface expression of CD11b and CD 18 and up regulated A 549 cell surface expression of CD 54 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In a group of 18 subjects with acute deep venous thrombosis ( DVT ) , evidenced by clinical examination and echo color Doppler , we examined the phenotypical expression of the polymorphonuclear leukocyte ( PMN ) beta 2 integrins ( CD11a , CD11b , CD11c , CD 18 ) , obtained by using a flow cytofluorimeter . ^^^ In DVT subjects , at baseline , the phenotypical expression of CD11b was decreased and that of CD11c was increased when compared with normal controls ; no difference was found in CD11a and CD 18 expression . ^^^ In normal subjects PMN activation with both activators led to a constant increase of all PMN adhesion molecules ; in DVT subjects CD11b , CD11c and CD 18 increased , while CD11a expression did not show any change . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Expression of MCK 2 drives the mobilization of a population of leukocytes from bone marrow that express myeloid marker Mac 1 ( CD11b ) , intermediate levels of Gr 1 ( Ly 6 G / C ) , platelet endothelial cell adhesion molecule 1 ( PECAM 1 , CD 31 ) , together with heterogeneous levels of stem cell antigen 1 ( Sca 1 , Ly 6 A / E ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We have attempted to determine essential amino acids for these activities in the cytoplasmic regions of CD11b and CD 18 subunits by deletion mutagenesis . ^^^ Those deletions that lead to loss of adherence and enhanced apoptosis are truncation of CD11b before the MSEGG sequence ; CD 18 internal deletion of either the membrane proximal residues before the NPLF sequence or the NPLF sequence itself ; CD 18 truncation of the C terminal residues after the NPLF sequence . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Expression of CD 9 , CD11b , CD 18 , CD 52 and PDGFR beta in the interface membrane of loose endoprostheses . ^^^ Tissue samples from 16 wear particle induced and 20 infectious periprosthetic membranes were analyzed by reverse transcription polymerase chain reaction ( RT PCR ) , in situ hybridization , and immunohistochemistry with regard to the expression of CD 9 , CD11b , CD 18 , CD 52 , and platelet derived growth factor ( PDGFR ) beta . ^^^ RT PCR demonstrated cd 9 , cd11b , cd 18 , cd 52 , and pdgfr beta RNA in all samples . ^^^ Using RT PCR and immunohistochemical analysis , the cDNA microarray data of cd 9 , cd11b , cd 52 , and pdgfr beta could be validated , whereas the differential expression of cd 18 was not confirmed . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Comparison of infected PMNLs with uninfected cells showed a decrease of up to 50 % in the expression of CD11b , CD11c , and CD 18 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Wild type ( WT ) mice pretreated with neutralizing antibody against CD11a ( LFA 1 ) , CD11b ( Mac 1 ) , CD11a plus CD11b , or CD 18 alone failed to develop BP when injected with pathogenic anti mBP 180 IgG . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The resultant intracellular signaling causes the leukocyte surface expression of tissue factor and activation of leukocyte surface Mac 1 ( integrin alphaMbeta 2 , CD11b / CD18 ) . ^^^ Geometric mean fluorescence and per cent positive data were collected for each subpopulation to measure the binding of test antibodies directed at CD42a , tissue factor , coagulation FXa , bound fibrinogen , activated Mac 1 , and CD11b . ^^^ RESULTS : The physiologic agonists collagen and ADP increased monocyte platelet and neutrophil platelet aggregates and increased leukocyte surface Mac 1 / CD11b and surface bound tissue factor , FXa and fibrinogen . ^^^ Whereas the increases in Mac 1 / CD11b were mainly independent of leukocyte platelet binding , the increases in surface bound tissue factor , FXa and fibrinogen were mainly dependent on leukocyte platelet binding . ^^^ CONCLUSIONS : ( 1 ) We have developed novel whole blood flow cytometry assays to measure bound tissue factor , coagulation FXa , fibrinogen , activated Mac 1 and CD11b on the surface of monocytes and neutrophils , allowing independent analysis of monocytes and neutrophils with and without surface adherent platelets . ( 2 ) The monocyte and neutrophil surface binding of tissue factor , FXa and fibrinogen is mainly dependent on platelet adherence to monocytes and neutrophils , whereas the monocyte and neutrophil surface expression of CD11b and activated Mac 1 is mainly independent of platelet adherence to monocytes and neutrophils . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Critical requirement of CD11b ( Mac 1 ) on T cells and accessory cells for development of experimental autoimmune encephalomyelitis . ^^^ Mac 1 ( CD18 / CD11b ) is a member of the beta 2 integrin family of adhesion molecules and is implicated in the development of many inflammatory diseases . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
T cells expressed CD 18 and CD11a with no expression of CD11b , whereas neutrophils expressed CD 18 , CD11a , and CD11b . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Flow cytometry analysis of PMN demonstrated increased expression of CD 35 , CD11b and CD 18 at resting PMN surface , without any changes after fMLP stimulation . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Influence of sex steroids on the viability and CD11b , CD 18 and CD 47 expression of blood neutrophils from dairy cows in the last month of gestation . ^^^ Subsequently , it was hypothesized that 17beta estradiol modulates the expression of CD11b , CD 18 or CD 47 thereby explaining its influence on the migration of bovine neutrophils . ^^^ In addition , 17beta estradiol treatment of bovine blood neutrophils significantly decreased the expression of CD 47 ( P < 0 . 05 ) but not of CD11b or CD 18 . ^^^ Moreover , our results concerning the influence of 17beta estradiol on the CD11b , CD 18 and CD 47 expression extend previous demonstrations of the suppressive effect of 17beta estradiol on neutrophils migration and indicate that the altered expression of CD 47 may contribute to this phenomenon . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Stimulation of monocyte adhesion to endothelial cells by NEFA was inhibited by addition of neutralizing antibodies to either CD11b or CD 18 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
RESULTS : ATG treatment reduced the percentage of lymphocytes gated for CD 18 and CD62L , as well as the expression of CD11b , CD 18 , and CD62L in a dose dependent manner . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The expression of activated leukocyte adhesive molecules ( CD11b and CD 18 ) and cytokine inducible leukocyte endothelial adhesive molecule ( CD62P ) were studied by flow cytometry . ^^^ CONCLUSIONS : Our investigation indicated that the blood processing in cell saver alone did not increase the expression of either CD11b , CD 18 , or CD62P , and the levels of TNF alpha , IL 6 and IL 8 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
CD 18 , the common beta subunit of beta 2 integrins , associates with four distinct alpha chains to give rise to four different beta 2 integrins : CD11a / CD18 ( LFA 1 ) , CD11b / CD18 ( Mac 1 ) , CD11c / CD18 ( CR 4 ) , and CD11d / CD18 . ^^^ Co expression of CD11b and CD 18 of cattle on Lkt resistant cells is necessary to irrefutably demonstrate the role of Mac 1 in Lkt induced cytolysis . ^^^ CD 18 , the common beta subunit of beta 2 integrins , associates with four distinct alpha chains to give rise to four different beta 2 integrins : CD11a / CD18 ( LFA 1 ) , CD11b / CD18 ( Mac 1 ) , CD11c / CD18 ( CR 4 ) , and CD11d / CD18 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In this study , we examined the pathophysiological role of the beta ( 2 ) integrins CD 18 , CD11a , and CD11b in the pathogenesis of dextran sodium sulfte ( DSS ) induced experimental colitis . ^^^ Examination of specific leukocyte populations in the distal colon from various mice revealed significant attenuation of neutrophil and macrophage infiltrates in CD 18 , CD11a , and CD11b null mice . ^^^ This study demonstrates that genetic loss of CD 18 or CD11a is protective during experimental colitis and that CD11b may serve a regulatory role during development of disease . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
UK 279 , 276 binds to the CD11b / CD18 ( MAC 1 ) on neutrophils and was under development for the treatment of ischaemic stroke . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Single cell analysis of the expression kinetics of the differentiation marker CD11b ( Mac 1 ) revealed all or none switch like behavior , in contrast to the seemingly graduated change of expression when measured as a population average . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
METHODS : We further analyzed PMN surface expression of the integrins CD11b and CD 18 as markers of PMN activation . ^^^ CD11b and CD 18 expression was essentially unaltered in all groups . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The expression of adhesion molecules was examined using flow cytometry in blood which was taken from the abdominal aorta and incubated with FITC labeled CD11b ( or CD 18 ) antibodies . ^^^ The expression of CD11b and CD 18 on the neutrophils was induced in LPS group , while it was reduced significantly in PNS group . ^^^ It was suggested that PNS could reduce leukocyte adhesion in venules under the inhibitory effect on the expression of adhesion molecules ( CD11b and CD 18 ) on neutrophils . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Expression of MAC 1 ( CD11b ) in acute myeloid leukemia ( AML ) is associated with an unfavorable prognosis . ^^^ We have studied the expression of the beta 2 integrin Mac 1 ( CD11b ) on mononuclear cells in 48 patients with AML at first diagnosis by flow cytometry using a direct fluorescein conjugated antibody . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
To address this question , we infected knockout ( KO ) mice that have defective expression of CD11a , CD11b , CD 18 , CD 62 , CD 103 , or beta 7 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
RESULTS : Flow cytometric expression of CD 35 and CD11b , but not of CD62L or CD 18 , was increased in severe asthma . ^^^ F met leu phe increased CD11b , CD 35 and CD 18 and decreased CD62L expression in all groups , with a greater CD 35 increase in severe asthma . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
METHODS : IL 8 mediated PMN migration and expression of CXCR 1 , CD11b , and CD 18 was studied in isolated and whole blood PMN in normal controls . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Hemorrhage increases adhesion of leukocytes to the venular endothelium , mediated by increased expression of the Mac 1 integrin complex ( CD18 / CD11b ) present on leukocytes . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Among them , CD 18 and its ligand ICAM 1 are particularly intriguing because CD 18 and its heterodimer binding chains CD11a and CD11b were correspondingly expressed in ex vivo expanded EPCs isolated from rat and murine bone marrows . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Neutrophils were freshly isolated from blood of 10 male healthy volunteers , stimulated the same way as endothelial cells and analyzed for surface expression of L selectin , CD11b and CD 18 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The effect of leukocyte depleting filters on the total and activated leukocyte counts and the expression of surface adhesion molecules CD11b , CD 18 , and CD62L during the in vitro extracorporeal circulation of human blood was studied . ^^^ The expression of CD11b , CD 18 , and CD62L was measured with flow cytometry . ^^^ In the filtered group , the expression of CD11b , CD 18 , and CD612L decreased by 60 % , 21 % , and 79 % , respectively , and in the control group it increased by 24 % , 6 % , and 28 % ( p < 0 . 0001 ) . ^^^ Leukocyte depleting filters preferentially remove activated leukocytes and reduce the expression of CD11b , CD 18 , and CD62L during the in vitro extracorporeal circulation of human blood . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Tissue parts directly adjacent to the site of RNA isolation were analyzed immuno / histopathologically in order to overcome the problem of tissue heterogeneity . 34 genes were found constantly differentially expressed , among which were cd 9 , cd11b , cd 18 , cd 68 , osteopontin , ferritin heavy chain upregulated in the particle induced membrane and collagen types 1alpha 1 , 3alpha 1 , integrin alpha 1 , thrombospondin 2 and nidogen upregulated in the infectious membrane . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Neither chemokine caused mechanical or thermal hyperalgesia as determined by the Randall Selitto and Hargreaves test , respectively , despite comparable expression of activation markers ( i . e . , CD11b , CD 18 , and L selectin ) on infiltrating PMN . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Leukocyte adhesion receptors ( LFA 1 ; Mac 1 ; p 150 , 95 ) are a family of heterodimeric cell surface adhesion molecules expressed exclusively in granulocytes , lymphocytes , and macrophages . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Plasmodium falciparum infected erythrocytes bind ICAM 1 at a site distinct from LFA 1 , Mac 1 , and human rhinovirus . ^^^ The ICAM 1 binding site is distinct from those recognized by LFA 1 , Mac 1 , and the human major type rhinoviruses . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The expression of LFA 1 ( CD11a / CD18 ) , a receptor for ICAM 1 , and other leukocyte adhesion molecules , including Mac 1 ( CD11b / CD18 ) and p 150 , 95 ( CD11c / CD18 ) , was only maintained by IL 2 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Dendritic cells displayed high forward and low intermediate side angle scatter , and expressed high levels of major histocompatibility complex ( MHC ) class 1 and class 2 molecules , the heat stable antigen ( HSA ) , the adhesion molecules Pgp 1 ( CD 44 ) , LFA 1 , ICAM 1 and low levels of Mac 1 and the leukocyte common antigen CD 45 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The role of the cytokines IL 1 alpha , IL 1 beta , and IL 6 and the cell adhesion molecules ICAM 1 , LFA 1 ( alpha and beta ) , and Mac 1 as accessory molecules for stimulation of T cells by the superantigen staphylococcal enterotoxin B ( SEB ) was examined . ^^^ The cell adhesion molecules ICAM 1 and LFA 1 but not Mac 1 also functioned as accessory molecules for SEB induced cluster formation and lymphocyte blastogenesis . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The leukocyte integrins LFA 1 , Mac 1 and p 150 , 95 ( CD11a / CD18 , CD11b / CD18 , CD11c / CD18 ) mediate crucial leukocyte adhesive functions in immune and inflammatory reactions . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Expression of the beta 2 integrins ( LFA 1 , CD11a / CD18 ; MAC 1 , CD11b / CD18 ; p 150 , 95 , CD11c / CD18 ) and ICAM 1 ( CD 54 ) by monocytes and granulocytes was studied using flow cytometry . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule 1 ( ICAM 1 , CD 54 ) is a cell adhesion molecule that interacts with the leukocyte beta 2 integrins , LFA 1 and Mac 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Stimulation of cells with antibodies to the monocyte surface Ag MAC 1 , LFA 1 , and ICAM 1 did not result in M CSF secretion . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
An immunodeficiency disease called `` leukocyte adhesion deficiency ( LAD ) ' ' that is defective in expression of leukocyte adhesion molecules of integrin family , LFA 1 , Mac 1 and p 150 , 95 , is discussed with special reference to its clinical features , laboratory findings , neutrophil functions , lymphocyte functions and molecular defects . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The adhesion and subsequent migration of freshly isolated NK cells was mainly mediated by CD11b / CD18 , because migration could be inhibited by 80 + / 8 % anti CD11b ( Mac 1 ) antibodies but not with antibodies against CD11a ( LFA 1 ) or CD11c ( p 150 , 95 ) , the other alpha chains of the beta 2 integrins . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Homogeneous marrow stromal cell monolayers capable of supporting proliferation of cocultivated CD34hi cells expressed VCAM 1 and fibronectin ( ligands for VLA 4 and VLA 5 ) as well as ICAM 1 ( ligand for LFA 1 and Mac 1 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Surface marker analysis showed an increased expression of F4 / 80 and Mac 1 on monocytes , Mac 1 expression on granulocytes and LFA 1 expression on resident peritoneal macrophages . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The contribution of LFA 1 ( CD11a / CD18 ) and MAC 1 ( CD11b / CD18 ) to the in vivo migration of polymorphonuclear leucocytes to inflammatory reactions in the rat . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Down modulation of surface Mac 1 on macrophages following adherence to anti Mac 1 or anti CD 18 coated surfaces also inhibited uptake of GBS . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
LFA 1 , Mac 1 , and p 150 , 95 comprise a family of cell surface glycoproteins that mediate adhesive interactions of myeloid and lymphoid cells . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Leukocyte adhesion deficiency is a disorder with mutations of the gene for the beta subunit , a component common to three adhesion molecules ; LFA 1 , Mac 1 and p 150 , 95 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
VLA 2 , VLA 5 , VLA 6 , LFA 1 , Mac 1 , p150 / 95 , beta 2 chain , HCAM , ICAM 1 , NCAM , and VCAM 1 were more expressed on ' normal ' as compared with ' leukemic ' bone marrow stromal cells , although this reached significance only for beta 2 chain and NCAM . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Leu CAMs ( CD11 / CD18 ) consisting of LFA 1 , Mac 1 , and p150 / 95 are leukocyte cell surface glycoproteins that are involved in various leukocyte functions . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Three leukocyte adhesion receptors have been described which mediate intercellular binding of leukocytes : LFA 1 ( CD11a / CD18 ) , Mac 1 ( CD11b / CD18 ) , and p150 / 95 ( CD11c / CD18 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Both the integrins LFA 1 and Mac 1 bind to ICAM 1 , an immunoglobulin superfamily member . ^^^ Here , we show that the binding site on ICAM 1 for Mac 1 is unexpectedly distinct from that for LFA 1 and maps to the third NH 2 terminal immunoglobulin like domain . ^^^ Agents that interfere with carbohydrate processing provide evidence that the size of the N linked oligosaccharide side chains on ICAM 1 affects binding to Mac 1 but not to LFA 1 . ^^^ Thus , we suggest that the extent of glycosylation on ICAM 1 may regulate adhesion to LFA 1 or Mac 1 in vivo . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
When administrated on day 6 after infection , antibody to the leukocyte integrin leukocyte function antigen 1 ( LFA 1 ) but not antibodies to MAC 1 , LECAM 1 ( the MEL 14 antigen ) , alpha 4 integrin or ICAM 1 dramatically reduced the incidence of CM , leading to survival of most mice until the later onset of anemia . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In 63 % of positive tubuli , intraluminal cells which expressed CD 18 , the common beta chain of leucocyte function associated antigen 1 , Mac 1 and p 150 , 95 , were present . 5 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Leukocyte integrin P 150 , 95 ( CD11c / CD18 ) functions as an adhesion molecule binding to a counter receptor on stimulated endothelium . p 150 , 95 is a member of the beta 2 family of integrins , which includes both LFA 1 and Mac 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The alpha subunits of each of the 3 members , lymphocyte function associated antigen 1 ( LFA 1 ) , macrophage antigen 1 ( Mac 1 ) and p 150 , 95 are designated CD11a , b and c respectively . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Adhesion of resting or activated neutrophils through the integrins LFA 1 and Mac 1 to ICAM 1 in a lipid bilayer does not occur at physiologic shear stresses ; however , static incubation of activated neutrophils allows development of adhesion that is greater than 100 fold more shear resistant than found on CD 62 . ^^^ Addition of a chemoattractant to activate LFA 1 and Mac 1 results in the arrest of neutrophils rolling on bilayers containing both CD 62 and ICAM 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We report the expression of LFA 1 and diethylaminoethyl ( DEAE ) Mac 1 alpha beta heterodimers on the cell surface of a fibroblastoid cell line , COS , by DEAE dextran cotransfection of the alpha and beta subunit cDNAs . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Neutrophil adhesion to interleukin 1 ( IL 1 ) stimulated human umbilical vein endothelial cells ( HUVEC ) involves the CD 18 family of leukocyte integrins ( lymphocyte function associated antigen 1 [ LFA 1 ] , Mac 1 , and p 150 , 95 ) and LECAM 1 ( DREG 56 / LEU 8 / LAM 1 antigen ) on neutrophils and intercellular adhesion molecule 1 ( ICAM 1 ) and endothelial leukocyte adhesion molecule 1 ( ELAM 1 ) on the endothelium . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
LFA 1 , Mac 1 , and p 150 , 95 are a family of functionally important leucocyte integrins that share a common beta subunit and participate in cellular adhesion . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
HIV 1 infection of target monocytes or T cells by cell free virus was blocked completely or partially by some mAb that prevent cell cell interactions ( CD 4 , HLA DR , LFA 1 , LFA 3 ) , but not by others ( ICAM 1 , MAC 1 , gp150 . 95 , CD 2 , CD 3 , CD 14 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The characteristic changes in the surface markers ( Mac 1 , LFA 1 , and F4 / 80 ) of peripheral monocytes were also investigated in order to analyze the mechanism of protection by M CSF . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Leukocyte adhesion deficiency ( LAD ) is an inherited immunodeficiency disease that is characterized by the deficient expression of the leukocyte adhesion glycoproteins lymphocyte function associated antigen 1 ( LFA 1 ) , Mac 1 , and p 150 , 95 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The monoclonal antibody IB 4 ( directed against CD 18 , the common beta subunit of structurally related adhesion receptors on phagocytes , LFA 1 , Mac 1 , and P 150 , 95 ) prevented the OZ mediated increase in PMN adherence to endothelial cells and the increase in endothelial permeability to 125I albumin . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We assessed the ability of a monoclonal antibody ( MoAb ) ( hybridoma : SP2 / 0 Ag . 14XBALB / c spleen cells ; isotype : murine IgG 1 ) to CD 18 that recognizes the beta chain of LFA 1 ( CD11a / CD18 ) , MAC 1 ( CD11b / CD18 ) and CD11c / CD18 to effect the neutrophils response to the proinflammatory chemotaxins leukotriene B 4 ( LTB 4 ) and 12 ( R ) hydroxy 5 , 8 , 11 , 14 eicosatetraenoic acid [ 12 ( R ) HETE ] in the mouse dermis . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Transfected murine L cells or monkey COS cells expressing human ICAM 1 bind to purified Mac 1 in a specific and dose dependent manner ; the attachment to Mac 1 is more temperature sensitive , lower in avidity , and blocked by a different series of ICAM 1 mAbs when compared to LFA 1 . ^^^ In a reciprocal assay , COS cells cotransfected with the alpha and beta chain cDNAs of Mac 1 or LFA 1 attach to immunoaffinity purified ICAM 1 substrates ; this adhesion is blocked by mAbs to ICAM 1 and Mac 1 or LFA 1 . ^^^ Two color fluorescence cell conjugate experiments show that neutrophils stimulated with fMLP bind to HUVEC stimulated with lipopolysaccharide for 24 h in an ICAM 1 , Mac 1 , and LFA 1 dependent fashion . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Pretreatment of PMN with IB 4 , a MAb to the CD 18 integrin , the common beta subunit of the adhesion proteins LFA 1 , Mac 1 , and p 150 , 95 of PMN , reduced the increases in PMN adherence and the endothelial monolayer permeability induced by the 6 hr TNF alpha treatment . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
With respect to the mechanism underlying defective macrophage adherence in EFA deficiency , no change in the expression of cell surface adherence molecules ( Fc receptor , Mac 1 , or LFA 1 ) was noted with the deficiency state . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Leukocyte adherence is mediated by a superfamily of glycoproteins denoted LFA 1 ( the lymphocyte function associated antigen 1 ) , Mac 1 ( macrophage antigen 1 ) and p 150 , 95 . ^^^ The pkC stimulated adherence of monocytes to endothelium was inhibited by the presence of a monoclonal antibody to Mac 1 , while monoclonal antibodies to p 150 , 95 and LFA 1 did not influence adherence . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
This rat alpha 1 sequence also contains a 206 amino acid 1 domain , inserted between repeats 2 and 3 , that is homologous to 1 domains found in the same position in the alpha subunits of several integrins ( VLA 2 , Mac 1 , LFA 1 , p 150 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The CD 11 complex is part of a larger complex composed of four glycoproteins ( CDw 18 ) precipitated by MoAb 60 . 3 , with properties suggesting that the CDw 18 complex is equivalent to the Mac 1 , LFA 1 , p 150 , 95 glycoprotein family implicated in adherence dependent leukocyte functions . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Regulated expression of the Mac 1 , LFA 1 , p 150 , 95 glycoprotein family during leukocyte differentiation . ^^^ The regulation of Mac 1 , LFA 1 , and p 150 , 95 expression during leukocyte differentiation was examined . ^^^ The resultant Mac 1 , LFA 1 , p 150 , 95 phenotype resembled hairy cell leukemia , a B cell plasmacytoid leukemia . ^^^ These cells acquired a Mac 1 , LFA 1 , p 150 , 95 profile that resembled that of granulocytes , with more Mac 1 than p 150 , 95 induced . ^^^ The contributions of Mac 1 , LFA 1 , and p 150 , 95 to aggregation of PMA differentiated U 937 cells were assessed . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Moreover , the NH 2 terminal protein sequence of the 125 kDa chain was homologous with the NH 2 terminal sequences of two other cell surface proteins , lymphocyte function associated antigen 1 ( LFA 1 ) and macrophage antigen 1 ( Mac 1 ) , which have been implicated as receptors for adhesion proteins of leukocytes . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The molecule recognized by KB 90 appears to correspond to the p150 / 95 molecule in the family of leucocyte differentiation antigens of which the LFA 1 and Mac 1 molecules are members . ^^^ These reactions are clearly distinct from those of the LFA 1 and Mac 1 molecules . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Serum E 27 appears to be directed at determinants of the LFA 1 alpha chain and not the beta chain since it immunoprecipitated LFA 1 molecules but not the Mac 1 molecules . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Using immunoprecipitation , sodium dodecyl sulfate gel electrophoresis , immunofluorescence and flow cytometry , we have studied the expression of LAR in human CD5+ B cells from chronic lymphocytic leukemia ( B CLL ) patients . 170 , 95 kDa Mac 1 and p 150 , 95 , as well as 180 , 95 kDa LFA 1 heterodimers are shown to be co expressed on CD5+ mu + kappa + B cells from a patient where they retain their known function in homotypic cell cell adhesion . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Function and regulation of the neutrophil MEL 14 antigen in vivo : comparison with LFA 1 and MAC 1 . ^^^ The CD11 / 18 ( LFA 1 , Mac 1 ) molecules participate in neutrophil adhesion to cultured endothelium in vitro and are critical for effective neutrophil localization into inflamed tissues in vivo . ^^^ In contrast , Mac 1 is up regulated on inflammatory neutrophils and little change is seen in the level of LFA 1 expression . ^^^ Anti LFA 1 , anti Mac 1 , or MEL 14 antibody given i . v . suppress neutrophil accumulation within the inflamed peritoneum ( 38 % , 30 % , and 37 % of medium control , respectively ) without affecting the levels of circulating neutrophils . ^^^ Finally , in ex vivo adhesion assays of neutrophil binding to high endothelial venules in inflamed lymph node frozen sections MEL 14 inhibits greater than 90 % . anti LFA 1 20 to 30 % and anti Mac 1 less than 10 % of the binding of bone marrow neutrophils to inflamed lymph node high endothelial venules . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Cell to cell contact enhances the proliferation of acute myeloid leukemia ( AML ) cells in vitro without an apparent role of adhesion glycoproteins LFA 1 , MAC 1 and P 150 95 . ^^^ The role of the leukocyte function antigens ( LFA ) LFA 1 , Mac 1 , P 150 95 in this phenomenon was then analyzed , as these structurally related glycoproteins are involved in reactions requiring contact between hematopoietic cells . ^^^ LFA 1 , Mac 1 and P 150 95 were expressed on the AML cells of 15 , 6 and 8 patients , respectively . ^^^ Thus , no direct role for LFA 1 and / or Mac 1 and / or P 150 95 antigens in mediating contact induced AML proliferation could be demonstrated . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The antiserum reacted with p 150 , 95 and two other antigens with the same beta subunit , Mac 1 and lymphocyte function associated antigen 1 ( LFA 1 ) . ^^^ While LFA 1 appears very early in neutrophil maturation , it is becoming clear that Mac 1 and p 150 , 95 are upregulated from an intracellular storage pool of peroxidase negative granules that appear during the myelocyte stage of differentiation . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We present evidence that SGP 140 is distinct from the glycoprotein family ( Mac 1 , LFA 1 , and p150 / 95 [ CDw 18 ] ) that performs adhesive reactions of leukocytes . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Conjugation was also inhibited by an antibody ( MHM 23 ) recognizing the beta chain shared by LFA 1 , MAC 1 , and P150 / 95 molecules . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
LFA 1 , an antigen involved in cytolytic T lymphocyte mediated killing , and Mac 1 , the receptor for complement component C3bi , constitute a family of structurally and functionally related cell surface glycoproteins involved in cellular interactions . ^^^ In both mouse and man , Mac 1 ( OKM 1 ) and LFA 1 share a common 95 kDa beta subunit but are distinguished by their alpha chains , which have different cellular distributions , apparent molecular masses ( 165 and 177 kDa , respectively ) , and peptide maps . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Patients with recurring , life threatening bacterial infections have been identified who have moderate ( 95 % ) or severe ( greater than 99 % ) deficiency of Mac 1 and of the related LFA 1 and p 150 , 95 molecules . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Their overall structures and biochemical properties are similar to those of a group of vertebrate receptors , including those for fibronectin , fibrinogen and vitronectin , and also the leukocyte antigens Mac 1 , LFA 1 and p 150 , 95 and the VLA family of cell surface antigens . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
These receptors are not part of the Mac 1 , LFA 1 , p ( 150 , 95 ) family , but do have homology to the platelet Fn receptor , gpIIb / IIIa . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
A new finding is the blockage of helper T cell proliferation by anti Mac 1 as well as with anti LFA 1 antibodies , showing that accessory molecules may be as important as specific recognition of class 2 antigen molecules in the control of thymocyte proliferation and hence in thymocyte selection . ^^^ Mac 1 , like LFA 1 , belongs to a novel family of differentiation antigens involved in cell interactions . ^^^ The mechanism of syngeneic proliferation inhibition by anti Ia , anti Mac 1 , and LFA 1 antibodies may be the prevention of IL 2 receptor expression on thymocytes , and / or the inhibition of IL 2 secretion . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We have isolated cDNA clones encoding the beta subunit of the human LFA 1 , Mac 1 , and p 150 , 95 family of leukocyte adhesion proteins . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In addition , the alpha subunits from the LFA 1 , Mac 1 ( CR 3 ) , and p 150 , 95 family of heterodimers , the vitronectin receptor platelet GPIIb / IIIa family , and a position specific ( PS ) antigen important in Drosophila embryogenesis each showed average homologies of 31 40 % to individual VLA alpha sequences and 46 52 % homology to VLA alpha subunits including conservative substitutions . ^^^ Taken together , these results suggest that the VLA proteins , the LFA 1 , Mac 1 , and p 150 , 95 family , the GPIIb / IIIa , vitronectin receptor family , and the Drosophila PS antigens have evolved as four subgroups in a highly conserved supergene family of receptors involved in fundamentally important functions , such as cell adhesion , migration , and embryogenesis . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The functional role of the LFA 1 molecule in the interaction between helper T lymphocytes and B lymphocytes was investigated using lymphocytes from patients with leukocyte adhesion deficiency , an inherited immunodeficiency characterized by a defective leukocyte expression of the LFA 1 , Mac 1 ( CR 3 ) and p 150 , 95 molecules . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Fibrinogen association with human monocytes : evidence for constitutive expression of fibrinogen receptors and for involvement of Mac 1 ( CD 18 , CR 3 ) in the binding . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Chromosomal location of the genes encoding the leukocyte adhesion receptors LFA 1 , Mac 1 and p 150 , 95 . ^^^ The adhesion receptors Mac 1 , LFA 1 , and p 150 , 95 are cell surface alpha / beta heterodimers that play a key role in leukocyte adhesion processes . ^^^ The genes for Mac 1 , LFA 1 , and p 150 , 95 alpha subunits have been located to chromosome 16 by means of Southern blot analysis using a series of somatic cell hybrids . ^^^ The gene encoding the LFA 1 / Mac 1 / p150 , 95 beta subunit , and defective in leukocyte adhesion deficiency , has been located on chromosome 21 , band q 22 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The expression of Mac 1 ( 3 , 4 fold increase ) was consistently enhanced more than p 150 , 95 ( 1 . 4 fold increase ) and LFA 1 expression was unchanged . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The glycosylation and polypeptide chain length of the p 150 , 95 , Mac 1 , and lymphocyte function associated antigen ( LFA 1 ) alpha and beta subunits were compared by immunoprecipitation with subunit specific MAb and antisera , and by digestion with Endo H and N glycanase . ^^^ Mac 1 and LFA 1 alpha subunits have precursors of 160 , 000 Mr and 165 , 000 Mr , respectively , and contain N linked carbohydrates . ^^^ The polypeptide chain length for the Mac 1 alpha subunit is 137 , 000 Mr , and for LFA 1 is 149 , 000 Mr . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Periodontal manifestations of the heritable Mac 1 , LFA 1 , deficiency syndrome . ^^^ The clinical , histopathologic and functional consequences of the genetic deficiency of leukocyte Mac 1 , LFA 1 and p 150 , 95 were assessed among three affected patients , heterozygotes and unaffected individuals among two generations of a single kindred . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The leukocyte adhesion receptors , p 150 , 95 , Mac 1 and LFA 1 are integral membrane glycoproteins which contain distinct alpha subunits of 180 , 000 150 , 000 Mr associated with identical beta subunits of 95 , 000 Mr in alpha beta complexes . p 150 , 95 alpha subunit tryptic peptides were used to specify oligonucleotide probes and a cDNA clone of 4 . 7 kb containing the entire coding sequence was isolated from a size selected myeloid cell cDNA library . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Mac 1 , LFA 1 human leukocyte adhesion glycoprotein family . ^^^ Monoclonal antibodies specific for p 150 , 95 , a third member of the Mac 1 and lymphocyte function associated antigen 1 ( LFA 1 ) leukocyte adhesion protein family , have been identified and used to study the biochemistry and cellular expression of p 150 , 95 . p 150 , 95 is a noncovalently associated heterodimer containing alpha 10 and beta subunits of Mr = 150 , 000 and 95 , 000 respectively . ^^^ Findings suggest that the p 150 , 95 alpha 10 beta complex shares a common beta subunit with the alpha L beta LFA 1 and alpha M beta Mac 1 complexes . ^^^ Patients with a previously demonstrated genetic deficiency in Mac 1 and LFA 1 fail to express p 150 , 95 . ^^^ Deficiency of the Mac 1 , LFA 1 , and p 150 , 95 alpha beta complexes on the surface of patient cells appears due to a defect in the common beta subunit . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Binding of the adhesive protein complex ( LFA 1 / Mac 1 / p150 , 95 ) to concanavalin A . ^^^ Immunoprecipitation of the labeled proteins after solubilization in non ionic detergents , with monoclonal antibodies ( MAbs ) directed against a family of leukocyte membrane proteins ( LFA 1 , Mac 1 , p 150 , 95 , and the beta subunit these glycoproteins share ) , indicated that Mac 1 and p 150 , 95 were bound by Con A . ^^^ Similar experiments in a patient deficient in LFA 1 , Mac 1 , p 150 , 95 , and the beta subunit confirmed that Mac 1 alpha and the beta subunit were important Con A binding proteins . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
To further investigate cell surface molecules concerned with platelet function , antisera against mouse platelets , thymocytes , and macrophages and monoclonal antibodies against Mac 1 ( complement receptor type 3 ) and leukocyte function associated glycoprotein type 1 ( LFA 1 ) were used to demonstrate LFA 1 like molecules on mouse platelets . ^^^ The alpha subunits of platelet and thymocyte LFA 1 showed identical electrophoretic mobility but differed significantly from the alpha subunit of macrophage Mac 1 . ^^^ Peptide mapping demonstrated the identity of the beta subunits of these three molecules but showed that the alpha subunit of Mac 1 was distinct from the alpha subunits of platelet and thymocyte LFA 1 . ^^^ Human platelets , which are inactive in immune adherence assays , are shown to lack LFA 1 and Mac 1 . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
MAb directed at the alpha subunits of Mac 1 ( alpha M ) , LFA 1 ( alpha L ) , p 150 , 95 ( alpha 10 ) , or their common beta subunit were used to characterize the contributions of the Mac 1 glycoprotein family to granulocyte adherence reactions . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The mouse leukocyte adhesion proteins Mac 1 and LFA 1 : studies on mRNA translation and protein glycosylation with emphasis on Mac 1 . ^^^ Translation in vitro of mRNA and immunoprecipitation with specific rabbit antisera showed that the unglycosylated precursor polypeptides of the mouse Mac 1 and lymphocyte function associated antigen ( LFA 1 ) alpha subunits are 130 , 000 Mr and 140 , 000 Mr , respectively . ^^^ The Mac 1 and LFA 1 alpha subunit translation products are immunologically noncross reactive , showing that differences between these related proteins are not due to post translational processing . ^^^ Mac 1 and LFA 1 alpha subunits could only be in vitro translated from mRNA from cell lines the surfaces of which express the corresponding Mac 1 and LFA 1 alpha beta complexes , showing tissue specific expression is regulated at the mRNA level . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The importance of the Mac 1 , LFA 1 glycoprotein family in monocyte and granulocyte adherence , chemotaxis , and migration into inflammatory sites : insights from an experiment of nature . ^^^ The Mac 1 , LFA 1 ( lymphocyte function associated 1 ) , p 150 , 95 family of glycoproteins , which share a common beta subunit of Mr 95 000 , are of widespread importance in leucocyte adhesion reactions . ^^^ Antibodies to the common beta subunit , to the Mac 1 alpha subunit , and to a lesser extent to the LFA 1 and p 150 , 95 alpha subunits , inhibit many of the same functional responses by normal cells . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The genetic deficiency of leukocyte surface glycoprotein Mac 1 , LFA 1 , p 150 , 95 in humans is associated with defective antibody dependent cellular cytotoxicity in vitro and defective protection against herpes simplex virus infection in vivo . ^^^ The role of the Mac 1 , LFA 1 , p 150 , 95 leukocyte glycoprotein family in mediating antiviral host defense was investigated by utilizing mononuclear cells ( MC ) obtained from eight patients with a genetic deficiency of Mac 1 , LFA 1 , and p 150 , 95 , and normal MC incubated with subunit specific monoclonal antibodies ( MAb ) directed against these glycoproteins . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Differential subunit association affects the site specific glycosylation of the common beta chain from Mac 1 and LFA 1 . ^^^ This cell line simultaneously synthesizes two structurally related glycoproteins , Mac 1 and LFA 1 . ^^^ Mac 1 and LFA 1 each contain two subunits in noncovalent association in an alpha 1 beta 1 structure . ^^^ For both Mac 1 and LFA 1 , the association of the alpha and beta chains occurs prior to any Golgi mediated processing of the oligosaccharide moieties on either one of the subunits . ^^^ To evaluate the effects of differential subunit association on the site specific glycosylation of the beta chain , [ 3H ] glucosamine labeled oligosaccharides were isolated from the beta chain of Mac 1 and LFA 1 and were compared by a variety of enzymatic and chromatographic techniques . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The amino acid sequence of the amino termini of platelet GPIIb alpha and GPIIb beta and of the alpha chains of the leukocyte LFA 1 and Mac 1 glycoprotein complexes had significant sequence homology . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Patients with an inherited deficiency of the adherence glycoproteins LFA 1 , Mac 1 , and p 150 , 95 are unable to mobilize polymorphonuclear leukocytes ( PMNLs ) to peripheral sites of inflammation . ^^^ LFA 1 / Mac 1 / p150 , 95 deficient PMNL exhibited profoundly impaired movement stimulated by chemotactic factors when the cells were required to move over two dimensional surfaces . ^^^ No deficiency of translational mobility of PMNL from LFA 1 / Mac 1 / p150 , 95 deficient patients was observed in collagen gels . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Leukocyte adhesion deficiency : an inherited defect in the Mac 1 , LFA 1 , and p 150 , 95 glycoproteins . ^^^ These include Mac 1 ( complement receptor type 3 ) , lymphocyte function associated antigen 1 ( LFA 1 ) , and p 150 , 95 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Leukocyte adhesion deficiency ( LAD ) is a heritable disease involving deficient expression of three related leukocyte adhesion glycoproteins : LFA 1 , Mac 1 , and p 150 , 95 . ^^^ Heterogeneous mutations in the beta subunit common to the LFA 1 , Mac 1 , and p 150 , 95 glycoproteins cause leukocyte adhesion deficiency . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Three membrane proteins , Mac 1 , LFA 1 , and p 150 , 95 each consisting of a specific alpha subunit and a beta subunit common to them , have been reported to be deficient in neutrophils from several patients with a neutrophil adhesion disease . ^^^ Using monoclonal antibodies to Mac 1 alpha , LFA 1 alpha , and to the beta subunit , it was deduced that the siblings ' neutrophils lacked the above three membrane proteins . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Sequence homology of the LFA 1 and Mac 1 leukocyte adhesion glycoproteins and unexpected relation to leukocyte interferon . ^^^ The lymphocyte function associated 1 ( LFA 1 ) and macrophage 1 ( Mac 1 ) glycoproteins mediate differing types of adhesion reactions on these cells . ^^^ LFA 1 participates in T lymphocyte and natural killer cell adhesion to target cells , whereas the Mac 1 antigen is identical to the complement receptor type 3 , which mediates adhesion of monocytes and granulocytes to C3bi sensitized particles . ^^^ LFA 1 and Mac 1 resemble one another in overall structure , having alpha subunits of relative molecular mass ( Mr ) 180 , 000 and 170 , 000 , respectively , which are non covalently associated with beta subunits of Mr 95 , 000 in alpha 1 beta 1 complexes . ^^^ Nonetheless , the shared beta subunit suggested that LFA 1 and Mac 1 might be members of a protein family containing diversified but evolutionarily related alpha subunits . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The LFA 1 , Mac 1 glycoprotein family and its deficiency in an inherited disease . ^^^ Soon after these molecules and at least some of their functions had been defined with monoclonal antibodies , an inherited disease , LFA 1 , Mac 1 deficiency , was discovered in humans . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Two well characterized antigens [ Mac 1 and lymphocyte function associated antigen ( LFA 1 ) ] , expressed on a variety of leukocytes , are members of a family of surface proteins associated with multiple recognition functions . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The function of LFA 1 in cell mediated killing and adhesion : studies on heritable LFA 1 , Mac 1 deficiency and on lymphoid cell self aggregation . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The severe and moderate phenotypes of heritable Mac 1 , LFA 1 deficiency : their quantitative definition and relation to leukocyte dysfunction and clinical features . ^^^ As shown with subunit specific monoclonal antibodies in immunofluorescence flow cytometry and 125I immunoprecipitation techniques , in addition to a NaB3H4 galactose oxidase labeling assay , granulocytes , monocytes , or lymphocytes from these individuals had a `` moderate ' ' or `` severe ' ' deficiency of Mac 1 , LFA 1 , or p 150 , 95 ( or a combination ) three structurally related `` adhesive ' ' surface glycoproteins . ^^^ The recognition of phenotypic variation among patients with Mac 1 , LFA 1 deficiency may be important with respect to therapeutic strategies . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Inherited deficiency of the Mac 1 , LFA 1 , p 150 , 95 glycoprotein family and its molecular basis . ^^^ The glycoproteins , Mac 1 , LFA 1 , and p 150 , 95 , have the subunit compositions alpha M beta , alpha L beta , and alpha 10 beta , respectively . ^^^ Using subunit specific monoclonal antibodies , both the alpha M and beta subunits of Mac 1 , the alpha L and beta subunits of LFA 1 , and at the least the beta subunit of p 150 , 95 , were found to be deficient at the cell surface by the techniques of immunofluorescence flow cytometry , radioimmunoassay , and immunoprecipitation . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
LFA 1 and Lyt 2 , 3 , molecules associated with T lymphocyte mediated killing ; and Mac 1 , an LFA 1 homologue associated with complement receptor function . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Mapping of antigenic and functional epitopes on the alpha and beta subunits of two related mouse glycoproteins involved in cell interactions , LFA 1 and Mac 1 . ^^^ Mouse Mac 1 , a complement receptor associated surface structure on macrophages , and LFA 1 , a function associated structure on lymphocytes , comprise a novel family of leukocyte differentiation antigens participating in adhesive cell interactions . ^^^ Mac 1 and LFA 1 contain alpha subunits of 170 , 000 and 180 , 000 Mr , respectively , and beta subunits of 95 , 000 Mr noncovalently associated in alpha 1 beta 1 complexes . ^^^ Cross reactive epitopes on Mac 1 and LFA 1 were found to be present on the beta subunits , which were immunologically identical . ^^^ Non cross reactive epitopes that are distinctive for Mac 1 or LFA 1 were localized to the alpha subunits . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Mac 1 NK that lysed Yac 1 displayed Qa 5 , LFA 1 , asialo GM 1 , Ly 5 . 1 , and NK 1 . 2 , but not Lyt 1 , Lyt 2 , Mac 2 , Mac 3 , or Mac 4 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Purification and structural characterization of LFA 1 , a lymphocyte function associated antigen , and Mac 1 , a related macrophage differentiation antigen associated with the type three complement receptor . ^^^ LFA 1 , an antigen associated with antigen specific T lymphocyte mediated killing , and Mac 1 , a macrophage differentiation antigen associated with type three complement receptor function , contain alpha chains of Mr = 180 , 000 and 170 , 000 , respectively , and beta chains of Mr = 95 , 000 . ^^^ The LFA 1 and Mac 1 beta chains are highly homologous or identical , whereas the alpha chains are highly different by tyrosyl tryptic peptide mapping ( Krzinger , K . , Ho , M . ^^^ T lymphoma cell lines express LFA 1 but not Mac 1 as shown by immunofluorescence and immunoprecipitation . ^^^ Conversely , some macrophage like lines express Mac 1 but not LFA 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
This study was designated to investigate the expression of three adhesion molecules ICAM 1 ( CD 54 ) , VCAM 1 , and ELAM 1 , and the counter ligands LFA 1 ( CD 18 ) , Mac 1 ( CD 11b / CD 18 ) , and VLA 4 ( CD 49d ) in frozen sections of endomyocardial biopsies ( EMB ) of heart allografts in relation to onset of CMV infection recorded as CMV antigenemia . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Four lines of evidence suggest that most are basophils : ( 1 ) The cells fulfill morphologic criteria for light microscopy . ( 2 ) Cell surface markers determined by immunofluorescence and flow cytometry revealed that the IgE bearing cells express the leukocyte antigens Fc gamma RII and the beta 2 integrins , LFA 1 and Mac 1 , but do not express the mast cell associated c kit receptor for stem cell factor . ( 3 ) The late phase histamine containing cells in late phase BAL fluids have the functional characteristics of basophils in their secretory responses to anti IgE , the f met peptide , and phorbol ester TPA . ( 4 ) The cells have a functional histamine type 2 receptor , a characteristic of basophils , not mast cells . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Most clones express Thy 1 , Pgp 1 , ICAM 1 , HSA and B 220 antigen , but are negative for LFA 1 , CD 2 , Mel 14 , Fc receptor , Mac 1 , CD 4 and CD 8 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In dermal vascular injury , a requirement for Mac 1 ( CD11b / CD18 ) but not for leukocyte function associated antigen 1 ( LFA 1 , CD11a / CD18 ) has been established . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In the sputum and peripheral blood eosinophils of asthmatics , Mac 1 was strongly expressed and LFA 1 , VLA 4 and sLe 10 were also expressed . ^^^ In addition , immunoreactivities of Mac 1 , LFA 1 , and VLA 4 were detected in eosinophils infiltrated into the bronchial submucosa , but sLe 10 was weakly detected . ^^^ These results suggest that binding between ICAM 1 and Mac 1 or LFA 1 , VCAM 1 and VLA 4 , not but ELAM 1 and sLe 10 , is mainly involved in eosinophil infiltration into the airway in allergic reaction such as bronchial asthma . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
No change in the expression of the beta 2 integrins Mac 1 and LFA 1 was apparent on peripheral blood neutrophils of the IL 8 transgenic mice . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Differential expression of ICAM 1 , VCAM 1 and their ligands LFA 1 , Mac 1 , CD 43 , VLA 4 , and MHC class 2 antigens in murine Toxoplasma encephalitis : a light microscopic and ultrastructural immunohistochemical study . ^^^ Microglia also expressed Mac 1 and leukocyte function associated antigen 1 ( LFA 1 ) , which are both ligands of ICAM 1 , as well as CD 45 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
RESULTS : Infiltrating lymphocytes expressed LFA 1 , LFA 2 , VLA 4 , and VLA 5 , whereas some of the macrophages were also positive for p150 / 95 and MAC 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Keratinocytes at sites of cutaneous inflammation have increased expression of intercellular adhesion molecule 1 ( ICAM 1 ) , a cytokine inducible adhesion molecule which binds the leukocyte integrins LFA 1 and Mac 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Quantitation of PMN adhesion molecules revealed a loss of L selectin ( 8 + / 5 % of control group , n = 3 ) , an increase in Mac 1 ( 776 + / 82 . 60 % of control group , n = 3 ) , and no change in LFA 1 when normal PMNs were incubated with plasma from rats pretreated with 4 LPS ( P < 0 . 05 ) . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
By immunohistochemical analysis , Mac 1 , LFA 1 , and VLA 4 were strongly positive in eosinophils and mononuclear cells infiltrated in the bronchial mucosa and submucosa . ^^^ Immunolocalization for ICAM 1 , the ligand of Mac 1 and LFA 1 , was detected in the endothelial cells of capillaries and venules , in the mononuclear cells in submucosa , and in the basal layer of the epithelium . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The expression of the adhesion molecules LFA 1 ( CD11a CD 18 ) , Mac 1 ( CD11b CD 18 ) , and LAM 1 on predialysis and intradialysis neutrophils ( PMN ) was analyzed by flow cytometry in 10 chronic hemodialysis patients ( CHD ) and compared with age matched normal controls . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Blocking mAbs to lymphocyte function associated antigen 1 ( LFA 1 ) , Mac 1 , and very late activation antigen 4 ( VLA 4 ) were used to evaluate the role of these integrins in the migration . ^^^ Migration to the joints was not inhibited by treatment of the animals with mAb to LFA 1 , Mac 1 , or VLA 4 alone , and was partially ( 50 % ) inhibited in only the most arthritic joint , the talar joint , by the combination of mAb to LFA 1 plus Mac 1 . ^^^ Treatment with the combination of the three mAbs to LFA 1 , Mac 1 , and VLA 4 completely eliminated monocyte migration to all joints and dermal inflammation . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
T lymphocytes and neutrophils tether and roll on plastic immobilized PNAd and E selectin at 1 . 8 dyn / cm2 wall shear stress , but fail to interact with immobilized ICAM 1 , a ligand for LFA 1 and Mac 1 , at the same flow rate . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The profound pathogenesis of the LAD has been defined as impairments in adhesion dependent leukocyte functions such as chemotaxis due to a lack of leukocyte cell surface expression of adhesion molecules of LFA 1 , Mac 1 and p150 / 95 , the beta 2 integrin . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Reactivities of YTA 1 to K 562 cells transfected with LFA 1 alpha and beta ( CD11a / CD18 ) cDNAs and to CHO cells transfected with Mac 1 ( CD11b / CD18 ) or p 150 , 95 ( CD11c / CD18 ) cDNAs strongly suggest that YTA 1 recognizes either LFA 1 alpha or an epitope formed by a combination of LFA 1 alpha and beta . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
It is therefore of interest to evaluate eosinophil activation via intercellular adhesion molecule 1 ( ICAM 1 ) and the beta 2 integrin family , namely CR 3 ( Mac 1 ) , lymphocyte function associated antigen ( LFA ) 1 alpha and LFA 1 beta , which are ligands for ICAM 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Activation of lymphocyte function associated molecule 1 ( CD11a / CD18 ) and Mac 1 ( CD11b / CD18 ) mimicked by an antibody directed against CD 18 . ^^^ Stimulation of neutrophils with CBR LFA 1 / 2 induces binding to ICAM 1 through activation of both LFA 1 and Mac 1 . ^^^ Activation of Mac 1 by CBR LFA 1 / 2 was further confirmed by stimulation of neutrophil binding to fibrinogen , a ligand for Mac 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In addition , consecutive sections were stained with MoAbs against LFA 1 , CD45RO ( UCHL 1 ) DR human leucocyte antigen ( HLA DR ) , CD11b / CD18 ( Mac 1 ) , and CD11c / CD18 ( p 150 , 95 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
EXPERIMENTAL DESIGN : We analyzed the immunohistochemical localization of the adhesion molecules ( ICAM 1 , LFA 1 , Mac 1 , VCAM 1 , VLA 4 , P and E selectins ) in IBD , stressing phenotypical changes of endothelial cells . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Cells with Langerhans ' cell histiocytosis morphology were stained for ICAM 1 , for the beta 1 integrins alpha 4 ( VLA 4 ) and alpha 5 ( VLA 5 ) , and for the beta 2 integrins LFA 1 , MAC 1 and p 150 , 95 . ^^^ They were less reactive than the cells of Langerhans ' cell histiocytosis for alpha 4 ( VLA 4 ) and LFA 1 , were positive for MAC 1 and p 150 , 95 and were characterized by prominent expression of the beta 1 integrins alpha 2 ( VLA 2 ) , alpha 3 ( VLA 3 ) and of VnR ( alpha v / beta 3 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Despite extensive differences in N linked glycosylation , ICAM 3 purified from lymphoid cells and from neutrophils supports adhesion of LFA 1 bearing cells equally well ; however , neither supports adhesion of Mac 1 or p 150 , 95 expressing chinese hamster ovary cell transfectants . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The leukocyte integrin Mac 1 ( CD11b / CD18 ) and its beta subunit ( CD 18 ) , but neither lymphocyte function associated antigen 1 nor very late activation antigen 4 , were upregulated on monocyte / macrophages elicited by CP 10 ( 42 55 ) , thioglycollate , and macrophage colony stimulating factor . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The new compounds were shown to inhibit neutrophil adherence under conditions in which adherence is mediated by Mac 1 ( CD11b / CD18 ) and LFA 1 ( CD11a / CD18 ) ; they thus appear to target beta 2 integrins in their antiadhesion activity . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Residues critical for human ICAM 1 binding to the beta 2 integrin leukocyte function associated antigen 1 ( LFA 1 ) are highly conserved between all species , whereas those residues demonstrated to play an important role in interaction of human ICAM 1 with macrophage activation complex 1 ( Mac 1 ) are not highly conserved . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In vivo blood monocyte migration to acute inflammatory reactions , IL 1 alpha , TNF alpha , IFN gamma , and C5a utilizes LFA 1 , Mac 1 , and VLA 4 . ^^^ The role of the monocyte integrins , Mac 1 , LFA 1 , and VLA 4 , on the adhesion of rat blood monocytes to rat microvascular endothelial cells in vitro and the importance of these receptors in monocyte migration to inflammation in vivo were evaluated . ^^^ Monocyte adhesion to cytokine ( IL 1 , IFN gamma , and TNF alpha ) stimulated endothelial cells was mediated by Mac 1 , LFA 1 , and VLA 4 , but Mac 1 appeared to be less important than LFA 1 or VLA 4 . ^^^ Anti Mac 1 mAb treatment had no effect , whereas anti LFA 1 inhibited migration to C5a and the cytokines by 20 to 40 % . ^^^ Blocking both Mac 1 and LFA 1 decreased monocyte accumulation by 50 to 70 % to all stimuli . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
This liver associated lymphocyte population also presented a significantly higher percentage of Mac 1 , ICAM 1 , and LFA 3 and an increased surface expression of LFA 1 , LFA 2 , and NCAM in comparison with peripheral blood lymphocytes . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
CD11a / CD18 ( LFA 1 ) , CD11b / CD18 ( Mac 1 ) and CD11c / CD18 ( p 150 , 95 ) were monitored before and after standard cold and heat tests in 8 females with Raynaud ' s Disease and 8 matched controls . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The analyzed molecules included the beta 1 integrin or VLA family VLA 1 6 , the beta 2 integrins or leukocyte integrins LFA 1 , Mac 1 , and p 150 , 95 , the immunoglobulins LFA 3 , CD 2 , ICAM 1 and VCAM 1 and the selectins ELAM 1 and GMP 140 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Similarly , blood derived human phagocytes were stimulated to express adhesion molecules ( LFA 1 , MAC 1 , p 150 , 95 , ICAM 1 ) , to synthesize TNF alpha and IL 2 , and to develop a natural killer activity . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We show that the neutrophils from localized juvenile periodontitis patients with decreased neutrophil chemotaxis and increased adherence exhibit increased expression of the CD11 / CD18 family of adherence molecules , Mac 1 , leukocyte function associated antigen ( LFA 1 ) and p 150 , 95 , as compared with neutrophils obtained from systemically and periodontally healthy controls . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Leukocyte adhesion deficiency ( LAD ) is an autosomal recessive disease caused by heterogeneous mutations within the gene encoding the common beta subunit ( CD 18 ) of the three leukocyte integrins LFA 1 ( CD11a / CD18 ) , Mac 1 ( CD11b / CD18 ) , and p 150 , 95 ( CD11c / CD18 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Our results demonstrate that whereas expression of major histocompatibility complex ( MHC ) molecules , CD 45 , Mac 1 , LFA 1 , B cell markers , macrophage markers and some T cell markers ( as CD 2 , CD 4 and CD 8 ) did not undergo changes in culture , the level of expression of the adhesion molecules VLA 4 and ICAM 1 , and the T cell markers CD 5 , CD 25 and Thy 1 increased after 14 h incubation at 37 degrees C . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In contrast PMNL expressed only very sparse surface CD11a , suggesting that another beta 2 integrin , Mac 1 , which shares a common beta chain with LFA 1 may be the more important PMNL counter receptor for ICAM 1 in the glomerulus . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule 1 ( ICAM 1 ) is one of three immunoglobulin superfamily members that bind to the integrins lymphocyte function associated 1 ( LFA 1 ) and Mac 1 on leukocytes . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The MMB3 . 19 line was derived from a B 6 mouse and expresses monocyte / macrophage markers , including Mac 1 , Mac 2 , F4 / 80 , and LFA 1 , in addition to H 2 class 1 and class 2 molecules . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
As reviewed above , leucocyte motility involves cell adhesion mediated by a class of molecules termed leucocyte integrins ( beta 2 integrins ) which vary in their alpha subunits and share CD 18 as a common beta subunit ( CD11a / CD18 = LFA 1 , CD11b / CD18 = Mac 1 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We have previously found that polymorphonuclear leukocyte ( PMNL ) migration to adjuvant arthritic joints of rats was only partially inhibited by mAbs to the adhesion molecules LFA 1 ( CD11a / CD18 ) and Mac 1 ( CD11b / CD18 ) , suggesting that there is a CD11 / CD18 independent mechanism for PMNL migration to inflamed joints . ^^^ This migration to the joints was not inhibited by mAbs to LFA 1 alone or mAbs to LFA 1 plus Mac 1 that almost completely inhibited PMNL accumulation in dermal inflammatory reactions induced by zymosan activated serum ( C5adesArg ) , endotoxin , IL 1 alpha , or TNF alpha in the same rats . ^^^ Intra articular injection of the cytokines IL 1 alpha and TNF alpha , but not IFN gamma , induced marked PMNL accumulation in the joints ; this was strongly inhibited by the treatment of rats with anti LFA 1 and anti Mac 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In vitro binding of the monocytes was inhibited by mAbs against ICAM 1 , LFA 1 , CD 18 , and MAC 1 ; MAC 1 did not influence lymphocyte binding , although the other mAbs were effective . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
As no Mac 1 beta chain specific ( CD 18 ) mAb were able to induce a respiratory burst in monocytes , despite being able to interact with Fc gamma R via their Fc regions , as detected by competition with mAb IV . 3 for binding to Fc gamma RII , we conclude that intracellular signalling via Mac 1 mAb Fc gamma RII complexes requires the alpha chain . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We distinguish four categories of markers , which are characteristic of : ( 1 ) macrophage precursors and immature macrophages ( ER MP 12 , ER MP 20 , ER MP 54 , ER MP 58 ) ; ( 2 ) mature macrophages in general ( F4 / 80 , BM 8 , Mac 1 , Mac 2 , ER BMDM 1 ) ; ( 3 ) macrophage subsets ( ER HR 3 , ER MP 23 , ER TR 9 , Forssman antigen , MOMA 1 , MOMA 2 , Monts 4 , SER 4 ) , and ( 4 ) IFN gamma stimulated macrophages ( H 2Ia , LFA 1 , ICAM 1 , 158 . 2 , MBR 2 , TM 2 , TM 4 , and TM 5 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The adhesion molecule ICAM 1 mediates leucocyte adhesion to target structures and other immune cells by binding with the leucocyte adhesion receptors LFA 1 and MAC 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Immunohistochemical staining was used to document the serial expression of Mac 1 , intercellular adhesion molecule 1 ( ICAM 1 ) , and lymphocyte function associated antigen 1 ( LFA 1 ) in eyes of C3H / HeN mice with endotoxin induced uveitis . ^^^ Intercellular adhesion molecule 1 was first expressed on the ciliary body epithelium 6 hr after endotoxin injection and Mac 1 and LFA 1 were expressed on infiltrating inflammatory cells 12 hr after endotoxin injection . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule , a ligand for the leucocyte integrins CD11a / CD18 ( LFA 1 ) and CD11b / CD18 ( Mac 1 ) , that plays an important role in a variety of inflammatory and immune mediated mechanisms , is strongly expressed in retroocular connective tissue from patients with Graves ' ophthalmopathy ( GO ) and involved in lymphocyte attachment to cultured retroocular fibroblasts via the ICAM 1 / LFA 1 mediated pathway . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Dendritic cells expressed major histocompatibility ( MHC ) class 1 and class 2 molecules , the leucocyte common antigen CD 45 , the rat leucocyte antigen OX 44 , the rat macrophage marker ED 1 , and the adhesion molecules Mac 1 , LFA 1 and ICAM 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Two minutes after protamine administration , CD 18 ( beta subunit of Mac 1 ) expression measured by immunofluorescence flow cytometry increased 128 + / 9 % ( mean + / SEM ) over preprotamine levels in control piglets but remained unchanged in NPC piglets ( 99 + / 2 % , P < 0 . 05 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Mac 1 and LFA 1 , members of the leukocyte or CD 18 integrin subfamily of adhesion molecules , rapidly change from a low avidity to a high avidity state on activation of neutrophils by various agonists . ^^^ We have focused on identifying cytoskeletal proteins that interact with the cytoplasmic domain of the beta subunit ( beta 2 or CD 18 ) common to the leukocyte subfamily of integrins , which include LFA 1 , Mac 1 , and p 150 , 95 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Leukocytes , including granulocytes and lymphocytes , from the LAD show defect in adhesion molecules of beta 2 integrin , LFA 1 , Mac 1 and p 150 , 95 , on its leukocyte membrane surface . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The role of the leukocyte adhesion molecules VLA 4 , LFA 1 , and Mac 1 in allergic airway responses in the rat . ^^^ The leukocyte adhesion molecules VLA 4 of the beta 1 integrin family , and LFA 1 and Mac 1 of the beta 2 family have a demonstrated role in leukocyte endothelial adherence and may play a role in airway inflammation in asthma . ^^^ We studied the effects of blocking VLA 4 ( CD49d / CD29 ) and both LFA 1 ( CD11a / CD18 ) and Mac 1 ( CD11b / CD18 ) airway responses and inflammation in rats . ^^^ Twelve rats were treated prior to challenge with anti rat VLA 4 monoclonal antibody ( mAb ) , 10 rats received both anti LFA 1 and anti Mac 1 mAb , and 14 rats received a control mAb . ^^^ The early response was significantly decreased in both the anti VLA 4 group ( 131 % baseline RL ) and the anti LFA 1 / Mac 1 group ( 118 % ; p < 0 . 05 ) compared with the control group ( 202 % ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Granulocytes from venous blood samples were separated on a Ficoll Hypaque gradient and the phenotypical expression of CD11a / CD18 ( LFA 1 ) , CD11b / CD18 ( Mac 1 ) and CD11c / CD18 ( p 150 , 95 ) was observed by double indirect immunofluorescence using specific monoclonal antibodies . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
They comprise 3 elements : LFA 1 ( CD11a / CD18 ) expressed at the surface of leucocytes , and in particular lymphocytes , M 01 or MAC 1 ( CD11b / CD18 ) , and p 150 . 95 ( CD11c / CD18 ) expressed only on granulocytes , monocytes and macrophages . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The panel of adhesion molecules studied included HLA ABC , HLA DR , CD 3 , LFA 1 , MAC 1 , ICAM 1 , PECAM 1 , VCAM 1 , and E selectin 1 . ^^^ RESULTS : The adhesion molecules ICAM 1 , HLA DR , PECAM 1 , CD 3 , VCAM 1 , LFA 1 , and MAC 1 were selectively expressed in areas of corneal inflammation . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Finally , both LFA 1 ( CD11a / CD18 ) and Mac 1 ( CD11b / CD18 ) beta 2 integrins were required as well as the endothelial adhesion molecules E selectin and ICAM 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The molecules we analyzed included the VLA family VLA 1 6 , the leukocyte integrins LFA 1 , Mac 1 and p 150 , 95 , the immunoglobulins LFA 3 , CD 2 , ICAM 1 and VCAM 1 and the selectin ELAM 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The leukocyte specific integrins CD11a / CD18 ( lymphocyte function associated antigen 1 ( LFA 1 ) ) and CD11b / CD18 ( complement type 3 receptor ( Mac 1 ) ) , which bind to the intercellular adhesion molecules ICAM 1 and ICAM 2 , play a key role in adhesion . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In this study we investigated the effect of EM on peripheral neutrophil adhesion molecules such as LFA 1 and Mac 1 obtained from six healthy subjects . ^^^ Pretreatment of neutrophils with each concentration ( 10 ng / ml approximately 100 micrograms / ml ) of EM resulted in no significant reduction in the expression of LFA 1 alpha , beta and Mac 1 . ^^^ These findings indicate that EM does not directly reduce the expression of LFA 1 alpha , beta and Mac 1 on peripheral neutrophil obtained from healthy subjects . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
To inhibit leukocyte sequestration directly , mice were treated with anti CD11a ( LFA 1 ) or anti CD11b ( Mac 1 ) before endotoxin challenge . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Receptors for the beta 2 integrin family ( LFA 1 and Mac 1 ) were not found , nor were receptors of the beta 3 integrin family , GPIIIa . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Rosetting of armed U 937 cells could be inhibited with MoAbs versus the alpha chains of CD11a ( LFA 1 ) , 11b ( Mac 1 ) , or 11c ( p 150 , 95 ) by 67 % , 70 % , and 61 % , respectively ( P < . 007 ) , whereas binding of ICs to U 937 cells was unaffected . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
A major portion of polymorphonuclear leukocyte and T lymphocyte migration to arthritic joints in the rat is via LFA 1 / MAC 1 independent mechanisms . ^^^ However , the role of CD11 / CD18 molecules and their subtypes , such as LFA 1 and MAC 1 , in leukocyte migration to arthritis is not known . ^^^ Anti MAC 1 combined with anti LFA 1 did not inhibit PMNL or T lymphocyte migration to joints any more than anti LFA 1 alone . ^^^ However , the addition of anti MAC 1 to anti LFA 1 further inhibited PMNL migration to acute dermal inflammation by up to 90 % . ^^^ Furthermore , while LFA 1 and MAC 1 on PMNL and LFA 1 on T lymphocytes mediate much of the migration to dermal inflammation , the migration of these cells to arthritis has a major LFA 1 / MAC 1 independent component . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
This adherence was likely mediated by the beta 2 integrin , Mac 1 , and could be specifically inhibited with monoclonal antibodies to ICAM 1 and to the integrin beta 2 subunit . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
After whole blood lysis preparation , leukocytes were incubated with fluorescent antibodies to label LAM CD 11A / 18 ( LFA 1 ) , CD 11B / 18 ( Mac 1 ) , CD 11C / 18 ( p150 / 95 ) , and CD 54 ( ICAM 1 ) ( Becton Dickinson , San Jose , CA ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
LFA 1 , and not Mac 1 , is crucial for the development of hyperreactivity in a murine model of nonallergic asthma . ^^^ Monoclonal antibodies ( mAbs ) directed against the alpha chains of LFA 1 ( FD441 . 8 ) and Mac 1 ( M1 / 70 ) were injected intravenously 2 h before and 2 h after the challenge . ^^^ Treatment with anti LFA 1 mAb totally inhibited the development of tracheal hyperreactivity measured 24 h after the challenge , whereas anti Mac 1 mAb had only a partial effect on this response . ^^^ The decrease in T cells in the blood , which was also evident 24 h after the challenge , was totally inhibited by treatment with anti LFA 1 , whereas anti Mac 1 had little effect . ^^^ The increase in the number of neutrophils in BALF at this time point was completely inhibited by both anti LFA 1 and anti Mac 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
METHODS : Immunohistochemical analysis was used to determine the distribution of the following adhesion molecules : lymphocyte function associated antigen 1 ( LFA 1 ; CD11a / CD18 ) , Mac 1 and p150 / 95 ( CD11bc / CD18 ) , intercellular adhesion molecule 1 ( ICAM 1 ) , and CD 44 in tissue sections from the ankle joints of rats with AIA . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Cytofluorimetric analysis of the NB 4 cell membrane molecules confirmed the increase under ATRA of VLA 4 , LFA 1 , MAC 1 and ICAM 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Blocking monoclonal antibodies ( mAbs ) to both LFA 1 and Mac 1 together , as well as a mAb to CD 18 , inhibited neutrophil accumulation in the inflamed joints by 50 75 % . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Mononuclear cells bearing CD 13 , CD 14 , CD 33 and HLA DR with macrophage like morphology were accumulated in the perivascular area and expressed VLA 4 , LFA 1 and Mac 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Here we show that LFA 1 and Mac 1 can bind to ICAM 1 in the mouse as well . ^^^ Interestingly , we observed that binding of murine LFA 1 dominates over Mac 1 for binding to ICAM 1 . ^^^ Using three different murine macrophage cell lines that express distinct levels of LFA 1 and Mac 1 on their cell surface , we could only detect Mac 1 dependent adhesion to ICAM 1 when little or no LFA 1 is expressed on the cell surface . ^^^ When LFA 1 and Mac 1 are expressed at similar levels , the LFA 1 / ICAM 1 interaction dominates over Mac 1 / ICAM 1 interaction , indicating that there is a competition of LFA 1 and Mac 1 for ICAM 1 binding . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Cross linking of the alpha subunits of the beta 2 integrins demonstrated that only cross linking of Mac 1 but not LFA 1 or gp150 / 95 triggered tyrosine signaling . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We have investigated the role of the Mac 1 integrin beta 2 in the development and maintenance of arthritis by means of in vivo administration of 5C6 monoclonal antibody ( mAb ) to block this receptor . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Leukocyte adhesion deficiency ( LAD ) is a rare inherited immunodeficiency that is characterized by deficiency of the beta 2 integrin leukocyte adhesion molecules Mac 1 , LFA 1 , and p 150 , 95 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule 1 ( ICAM 1 ) is a member of the immunoglobulin superfamily that interacts with two integrins , LFA 1 and Mac 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Furthermore , the adhesion strengthening interaction observed for healthy neutrophils could be specifically inhibited by monoclonal antibodies ( mAbs ) to Mac 1 , but not to lymphocyte function associated antigen 1 ( LFA 1 ; CD11a / CD18 ) or intercellular adhesion molecule 2 ( ICAM 2 ; CD 102 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The changes in expression of LFA 1 on monocytes , MAC 1 on granulocytes and p 150 , 95 ( CD11c / CD18 ) on monocytes and granulocytes during and after the operation did not reach statistical significance . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Our results also indicate that viral pathogenicity is associated with increased migration of inflammatory cells expressing VLA alpha 4 , LFA 1 alpha , Mac 1 alpha , ICAM 1 , and beta 2 integrin into the intestinal mucosa . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Granulocyte ( PMN ) and monocyte ( MNC ) expression of LFA 1 , Mac 1 , and CD 45 at the start ( t 0 ) , t 1 and t 2 was quantitated by flow cytometry analysis , after staining of the cells with specific fluorescinated monoclonal antibodies . ^^^ Again , no significant difference was found between NSBD and UPBD in LFA 1 , Mac 1 and CD 45 expression on PMN and MNC , during both CU and PAN HD . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule 1 ( ICAM 1 , CD 54 ) , an adhesion molecule of the immunoglobulin superfamily , is an endothelial cell surface ligand for such leukocyte integrins as lymphocyte function associated molecule 1 ( LFA 1 , CD11a / CD18 ) , Mac 1 ( CD11b / CD18 ) and CD 43 . ^^^ We examined the expression of ICAM 1 in renal tissues of Masugi nephritis rats and directly examined the role of ICAM 1 by administration of neutralizing monoclonal antibodies ( MAbs ) to rat ICAM 1 , LFA 1 alpha subunit ( LFA 1 alpha ) , beta subunit ( LFA 1 beta ) and Mac 1 alpha subunit ( Mac 1 alpha ) . ^^^ Proteinuria was significantly suppressed by the MAbs against ICAM 1 , Mac 1 alpha and LFA 1 beta . ^^^ These results indicate that both ICAM 1 / LFA 1 and ICAM 1 / Mac 1 pathways are involved in neutrophil infiltration into the glomeruli . ^^^ On the other hand , monocytic infiltration was prevented by the MAbs against ICAM 1 , LFA 1 alpha and LFA 1 beta but not by anti Mac 1 alpha MAb . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
OBJECTIVE : Because recruitment and retention of lymphoid cells appear to be critical components of the pathogenesis of lichen planus , we have compared the expression and distribution of a panel of vascular adhesion molecules ( ELAM 1 , P selectin , ICAM 1 , VCAM 1 , PECAM 1 , CD 34 ) and leukocyte adhesion molecule ligands ( LFA 1 , Mac 1 , VLA 4 , L selectin ) in biopsies of this disease . ^^^ Almost all cells in the lichen planus infiltrates stained positive for ICAM 1 , L selectin , LFA 1 , and VLA 4 , and large numbers of cells also exhibited VCAM 1 , PECAM 1 , and Mac 1 immunoreactivity . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Anti integrin ( LFA 1 , VLA 4 , and Mac 1 ) antibody treatment and acute cardiac graft rejection in the rat . ^^^ Current experiments were designed to examine the effect of blocking Mac 1 , an important monocyte adhesion receptor and mediator of monocyte migration , together with anti LFA 1 and anti VLA 4 antibodies on cardiac graft survival and on the graft rejection pattern . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Experiments using monoclonal antibodies to block LFA 1 , Mac 1 , and p 150 , 95 ( three integrins vital for cell cell interactions ) mimic BLAD symptomatology and have led to the discovery of the reciprocal Intercellular Adhesion Molecule ( ICAM ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
By using polarized monolayers of human intestinal epithelia ( T 84 ) , PMN transepithelial migration has been shown to be dependent on the leukocyte integrin CD11b / CD18 ( Mac 1 ) , but not on CD11a / CD18 ( LFA 1 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Its ligands are the membrane bound integrin receptors LFA 1 and Mac 1 on leukocytes , CD 43 , the soluble molecule fibrinogen , the matrix factor hyaluronan , rhinoviruses , and Plasmodium falciparum malaria infected erythrocytes . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Activated polymorphonuclear leukocytes induce constriction of canine coronary artery via Mac 1 , but not LFA 1 , and ICAM 1 . ^^^ The PMN induced vasoconstriction ( 5 10 10 ( 6 ) cells / ml : 98 + / 8 % of KCl induced constriction ) was inhibited when PMNs were pretreated with the monoclonal antibody against Mac 1 ( 54 + / 4 % of KCl induced constriction ) but not against LFA 1 against ICAM 1 ( 31 + / 2 % of KCl induced constriction ) . ^^^ These results suggest that activated PMNs may mediate adherent dependent constriction of canine coronary artery via Mac 1 ( CD11b / CD18 ) , but not LFA 1 ( CD11a / CD18 ) , with ICAM 1 . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
METHODS : The expression of the integrin VLA beta 1 , alpha subunits of the beta 2 integrins LFA 1 , Mac 1 , and p 150 , 95 , the members of the immunoglobulin family ICAM 1 and VCAM 1 , and the selectin ELAM 1 on corneas with bullous keratopathy ( BK ) secondary to intraocular surgery was studied immunohistochemically using an APAAP method . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
By flow cytometry , it could be shown that among several surface adhesion molecules ( ICAM 1 / CD54 , VLA 4 / CD49d , Mac 1 / CD11b , LFA 1 / CD11a , LFA 2 / CD2 , LFA 3 / CD58 , VCAM 1 ) , only the constitutively expressed immunoglobulin family member intercellular adhesion molecule 1 ( ICAM 1 ) is modulated by proinflammatory cytokines on HMC 1 mast cells . ^^^ This cytokine induced upregulation of ICAM 1 expression might reveal a putative regulatory mechanism of mast cell interaction with effector cells bearing the counterparts of ICAM 1 ( CD 54 ) , the molecules Mac 1 ( CD11b / CD18 ) and leukosialin ( CD 43 ) , and the principal ligand LFA 1 ( CD11a / CD18 ) . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
There were no significant differences , however , in the expression of CD 23 , CD 32 , Mac 1 and adhesion molecules ( ICAM 1 , leukocyte function associated antigen 1 alpha ( LFA 1 alpha ) , LFA 1 beta , very late antigen 4 ) on eosinophils of patients with strongyloidiasis positive for anti HTLV 1 antibodies in comparison with those negative for these antibodies . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Expression of very late antigen 4 ( VLA 4 ) , lymphocyte function associated antigen 1 ( LFA 1 ) and macrophage antigen 1 ( Mac 1 ) on eosinophils from patients with eosinophilia was increased compared with those from healthy subjects . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The H 4 expression pattern and biochemical features , together with cross blocking , co capping , co modulation , and immunoprecipitation preclearing experiments showed that H 4 is different from other known co stimulatory molecules such as CD 69 , CD 2 , Ly 6 , CD 25 , OX 40 , Mac 1 and LFA 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
To help define the role of adhesion molecules in HAM / TSP , we studied the expression of lymphocyte function associated antigen 1 ( LFA 1 ) , Mac 1 , very late antigen 4 ( VLA 4 ) , Sialyl Lewisx ( SLex ) , intercellular adhesion molecule 1 ( ICAM 1 ) , vascular cell adhesion molecule 1 ( VCAM 1 ) , endothelial leukocyte adhesion molecule 1 ( ELAM 1 ) and monocyte chemoattractant protein 1 ( MCP 1 ) in the spinal cord lesions of HAM / TSP . ^^^ Although the expression of ICAM 1 in the spinal cords was not distinctive between HAM / TSP and controls , infiltrating mononuclear cells in the spinal cords of HAM / TSP strongly expressed LFA 1 and Mac 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Experiments with monoclonal antibodies to block LFA 1 , Mac 1 , and p 150 , 95 ( three integrins vital for cell cell interactions ) mimic BLAD symptomatology and have led to the discovery of the reciprocal intercellular adhesion molecule ( ICAM ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The number of LFA 1 and Mac 1 positive macrophages within the nerves was significantly reduced when liposomes were injected shortly after nerve transsection . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
CD 18 is a subunit for three beta 2 integrin molecules ( Mac 1 , p 150 , 95 , LFA 1 ) , which are expressed on the plasma membrane of neutrophils . ^^^ CD 18 is a subunit for three beta 2 integrin molecules ( Mac 1 , p 150 , 95 , LFA 1 ) , which are expressed on the plasma membrane of neutrophils . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Four different combinations of monoclonal antibodies against rat ICAM 1 , CD 11a , and CD 18 were utilized to determine the relative importance of LFA 1 , Mac 1 , and ICAM 1 in a rat model of severe lung allograft reperfusion injury . ^^^ We conclude that LFA 1 , Mac 1 , and ICAM 1 are all important adhesion molecules in lung allograft reperfusion injury yet even with antibody blockade of all three there are additional mechanisms allowing for neutrophil influx into the lungs . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In this study , we observed PAF induced up regulation of inducible adhesion molecules Mac 1 and LFA 1 beta on eosinophils of atopic patients compared with non atopic and normal subjects . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The sections were also stained immunohistochemically for the vascular endothelial adhesion molecules ICAM 1 , ELAM 1 ( E selectin ) , and VCAM 1 , and for the leukocyte ligands for ICAM 1 : LFA 1 ( CD11a / CD18 ) and Mac 1 ( CD11b / CD18 ) . ^^^ Infiltrating monocytes and lymphocytes expressed the LFA 1 ligand and infiltrating PMN expressed the MAC 1 ligand . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
By recognizing a site distinct from that of beta 2 integrins Mac 1 or LFA 1 , fibrinogen binding to ICAM 1 may provide an alternative pathway of intercellular adhesion and / or modulate integrin dependent adherence during inflammation and vascular injury . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
To elucidate the impaired neutrophil function in patients with chronic renal failure , we analyzed the expression of the adhesion molecules , LAM 1 , LFA 1 , Mac 1 , gp150 / 95 and phagocytosis activity of neutrophils in predialysis and hemodialysis patients by flow cytometry . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Human intercellular adhesion molecule 1 ( ICAM 1 ) plays an important role in immune responses as the major specific ligand for the beta 2 integrins LFA 1 and Mac 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Flow cytometry analysis of the spleen cells removed at various times from M . intracellulare infected mice also indicated no change in the level of LFA 1 beta or MAC 1 , a finding comparable with that of the J774A . 1 cells . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In this study , we investigated the involvement of the adhesion molecules intercellular adhesion molecule 1 ( ICAM 1 ) , vascular cell adhesion molecule 1 ( VCAM 1 ) , very late activation antigen 4 ( VLA 4 ) , lymphocyte function associated antigen 1 ( LFA 1 ) and macrophage antigen 1 ( Mac 1 ) on eosinophil infiltration in the cutaneous late phase response ( LPR ) in OVA sensitized Balb / c mice by two approaches , immunostaining and inhibition assays with each monoclonal antibody . ^^^ Infiltrated eosinophils and mononuclear cells in the skin expressed Mac 1 ( eosinophils : 38 . 9 + / 1 . 55 % , mononuclear cells : 51 . 2 + / 2 . 15 % ) , LFA 1 ( eosinophils : 33 . 3 + / 0 . 95 % , mononuclear cells : 23 . 1 + / 1 . 07 % ) and VLA 4 ( eosinophils : 14 . 3 + / 1 . 6 % , mononuclear cells : 17 . 2 + / 1 . 38 % ) at 12 h . ^^^ On the other hand , pretreatment with anti mouse LFA 1 mAb or Mac 1 mAb did not significantly decrease the infiltration . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Migration of activated neutrophils into MEE was significantly inhibited by blocking surface expressed cell adhesion molecules ( CAM ) with anti CD11b ( Mac 1 alpha ) and anti CD 18 ( common beta 2 subunit ) antibodies ( P < 0 . 001 ) but not with anti CD11a ( LFA 1 alpha ) antibody . ^^^ In vitro regulation of neutrophil migration by beta 2 integrins ( LFA 1 and Mac 1 ) in patients with otitis media . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
At the initial biopsy , there was no difference in the degree of glomerular C3c deposition , glomerular intercellular adhesion molecule ( ICAM ) 1 expression , or the numbers of cells bearing leukocyte function associated antigen 1 ( LFA 1 ) , Mac 1 , and ICAM 3 between the two groups . ^^^ At the follow up biopsy , the degree of glomerular C3c deposition , and the numbers of cells bearing LFA 1 , Mac 1 , and ICAM 3 , were significantly decreased only in group A ( P < 0 . 01 , P < 0 . 001 , P < 0 . 001 , and P < 0 . 01 , respectively ) . ^^^ Neither LFA 1 nor Mac 1 positive cells were positively correlated with ICAM 1 expression . ^^^ These results suggest that the glomerular leukocytes , infiltrating through Mac 1 / complement interaction , express ICAM 3 by themselves , and that LFA 1 / ICAM 3 interaction might participate in the glomerular aggregation of leukocytes in MPGN type 1 . ^^^ In this study , we could not conclude that LFA 1 / ICAM 1 or Mac 1 / ICAM 1 interaction was involved in the leukocyte accumulation in this disease . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The inflammatory reaction of the glomeruli of the kidneys was studied by immunohistochemical methods using 4 microm cryostat sections stained with specific monoclonal antibodies against neutrophils ( polymorphonuclear cells , PMNs ) and monocytes ( MOs ) , and against the adhesion molecules ICAM 1 and VCAM 1 on the endothelium , and LFA 1 , MAC 1 , and VLA 4 on the leukocytes . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule 1 ( ICAM 1 , CD 54 ) is a member of the Ig superfamily and is a counterreceptor for the beta 2 integrins : lymphocyte function associated antigen 1 ( LFA 1 , CD11a / CD18 ) , complement receptor 1 ( MAC 1 , CD11b / CD18 ) , and p 150 , 95 ( CD11c / CD18 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The expression of intercellular adhesion molecule 1 ( CD 54 or ICAM 1 ) on epithelial cells during acute or chronic inflammation may favor the interaction between epithelial cells and leukocytes expressing the natural ligands of ICAM 1 , LFA 1 ( CD11a / CD18 ) , and Mac 1 ( CD11b / CD18 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
NIF specifically antagonized Mac 1 dependent adherence , but the peptide blocked neutrophil adherence that was dependent upon both Mac 1 and LFA 1 integrins . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The number of CD4+ T cells and macrophage antigen 1 ( Mac 1 [ + ] ) cells in the brain and spinal cord were not decreased by anti LFA 1 MAb treatment . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Using flow cytometry , high concentrations of ICAM 1 , VCAM 1 , and NCAM antigens were detected on undifferentiated ES cells , but their specific receptors , Mac 1 , LFA 1 , and VLA 4 , were not detected . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
These agents enhanced adhesion of PMN to human umbilical vein endothelial cells stimulated with tumor necrosis factor alpha ( TNF alpha ) , and this effect was also mediated by beta 2 integrins LFA 1 and Mac 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Notably , antibodies directed against VLA 4 , other beta 2 integrins ( Mac 1 and p 150 ) , or other potential LFA 1 counterstructures that were expressed on the cell surface ( ICAM 1 and ICAM 3 ) did not block aggregation . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Tethering of PMN on platelet ICAM 2 via LFA 1 ( alphaLbeta 2 ) was observed , which may facilitate the transition between rolling on selectins and Mac 1 dependent arrest . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In the present study , binding of beta amyloid with the leukocyte integrin Mac 1 , a cell surface receptor on microglia , was studied by observing ( 1 ) inhibition of beta amyloid ( beta 25 35 ) mediated release of nitric oxide from cultured microglial cells following exposure to monoclonal antibodies against Mac 1 ( anti CD 18 and anti CD11b ) and ( 2 ) competitive binding of fluorochrome labeled beta 25 35 with anti CD 18 or anti CD11b using fluorescent flow cytometry . ^^^ An interaction between beta amyloid and CD 18 of Mac 1 was evidenced by the suppressive action of beta 25 35 on Wt . 3 mediated release of nitric oxide and the synergistic action between OX 42 and beta 25 35 in inducing nitric oxide release from microglia . ^^^ These findings suggest that CD 18 of Mac 1 may play a role in beta amyloid mediated release of nitric oxide . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Kinetics of the expression of LFA 1 alpha / CD11a , LFA 1 beta / CD18 , Mac 1 / CD11b , VLA 4 / CD49d , ICAM 1 / CD54 and L selectin / CD62L was determined on splenic CD4+ and CD8+ T cells and macrophages by flow cytometry . ^^^ In the spleen , a sustained increase in LFA 1 expression and a temporary increase at day 7 in the expression of VLA 4 , Mac 1 and ICAM 1 were detected on CD8+ T cells . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The degenerating nerves were invaded by Mac 1 , LFA 1 , and F4 / 80 positive macrophages ; significantly lower numbers of macrophages were present in ICAM 1 deficient nerves . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Increased expression of neutrophil and monocyte adhesion molecules LFA 1 and Mac 1 and their ligand ICAM 1 and VLA 4 throughout the acute phase of myocardial infarction : possible implications for leukocyte aggregation and microvascular plugging . ^^^ METHODS : We measured the expression of neutrophil and monocyte lymphocyte function associated antigen 1 ( LFA 1 ) , Mac 1 , very late after activation antigen 4 ( VLA 4 ) and intercellular adhesion molecule 1 ( ICAM 1 ) by flow cytometry throughout the acute phase of acute myocardial infarction in 25 patients and 10 age matched control subjects . ^^^ The expression of LFA 1 , Mac 1 , VLA 4 and ICAM 1 on the monocyte cell membrane in patients with an acute myocardial infarction was increased compared with that in control subjects by 22 % ( on day 7 ) , 67 % , 13 % and 44 % ( all on day 4 ) , respectively ( all p < 0 . 001 ) . ^^^ In addition , the manifestation of increased density of LFA 1 and Mac 1 by activated leukocytes with monocytes also expressing ICAM 1 suggests that leukocytes may form microaggregates that could cause microvascular plugging . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule 1 ( ICAM 1 ) is an inducible glycoprotein that interacts with the leukocyte beta 2 integrins , LFA 1 and Mac 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Levels of L selectin , leukocyte functional antigen 1 ( LFA 1 ) , Mac 1 , and very late activation antigen 4 ( VLA 4 ) expression were determined on alphabeta and gammadelta T cells and monocytes by dual color immunofluorescent flow cytometry . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Cells infiltrating livers of IL 12 treated mice were primarily mononuclear leukocytes expressing LFA 1 , VLA 4 , MAC 1 , and CD 18 adhesion molecules but little L selectin . ^^^ Kupffer cells constitutively expressed LFA 1 and smaller amounts of MAC 1 , and high levels of ICAM 1 were constitutively expressed by liver sinusoidal lining cells , portal tract , and central vein endothelia . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Adhesion molecules such as ICAM 1 , Mac 1 , and LFA 1 may help regulate interactions of immune cells and inflammatory cells . ^^^ LFA 1 and Mac 1 were strongly expressed in these immune and inflammatory cells . ^^^ The increased expression of ICAM 1 is linked with massive infiltration of inflammatory cells that express LFA 1 and Mac 1 , and also with APCs that express HLA DR , suggesting that ICAM 1 exerts a key role in immuno inflammatory responses in gastric mucosa of patients with H . pylori associated gastritis . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In this study the contact between leukocytes and uncoated polyethylene terephthalate or pyrolytic carbon coated polyethylene terephthalate ( PET and PET PC , respectively ) was inspected by analyzing whether the expression of some adhesion molecules involved in leukocyte activation , namely LFA 1 ( CD11a / CD 18 ) , Mac 1 / CR3 ( CD11b / CD18 ) , and LECAM 1 ( CD62L ) can be modified . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Increase of cell attachment was dependent on lymphocyte function associated antigen 1 ( LFA 1 ) and on Mac 1 , since it could be blocked with antibodies against both respective integrin alpha chains . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
GM CSF induced adhesion was completely inhibited by incubation with monoclonal antibodies directed against the common beta 2 chain ( CD 18 ) of the LFA 1 , Mac 1 , p 150 , 95 complexes . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule 1 ( ICAM 1 ) is a ligand for the integrins lymphocyte function associated antigen 1 ( LFA 1 ) and complement receptor 3 ( Mac 1 ) , making it an important participant in many immune and inflammatory processes . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
METHODS : To investigate the mechanisms of intraglomerular leukocyte infiltration in IgA nephropathy ( IgA N ) and membranoproliferative glomerulonephritis type 1 ( MPGN 1 ) , we immunohistochemically examined the intraglomerular expression of leukocyte function associated antigen 1 ( LFA 1 , CD11a / CD18 ) , macrophage 1 ( Mac 1 , CD11b / CD18 ) and intercellular adhesion molecule 1 ( ICAM 1 , CD 54 ) together with glomerular deposition of C3c and fibrinogen . ^^^ The LFA 1 / ICAM 1 pathway may play an important role in glomerular leukocyte infiltration in IgA N , while the Mac 1 / complement pathway may be important in MPGN 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In the present study on idiopathic membranous glomerulonephritis ( MGN ) the expression of adhesion molecules ( ICAM 1 , VCAM 1 , PECAM 1 , E selectin , LFA 1 , Mac 1 ) was analyzed in different cellular compartments of the kidney using an indirect immunoperoxidase technique and monoclonal antibodies . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Furthermore , phosphoinositide turnover of IL 5 stimulated adherent eosinophils was also inhibited by anti CD 18 mAb , suggesting that cellular adhesion plays important roles in eosinophil signal transduction . alphaM beta 2 ( Mac 1 , CD11b / 18 ) was one of the beta 2 integrins involved in eosinophil adhesion to protein coated plates . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Here we investigated in parallel the role of beta 2 integrin family members , LFA 1 ( CD11a / CD18 ) and Mac 1 ( CD11b / CD18 ) on monocytes , and the immunoglobulin supergene family members , ICAM 1 and ICAM 2 on HSF and on human umbilical vein endothelial cells ( HUVEC ) , in monocyte migration through HSF and HUVEC monolayers . ^^^ Using function blocking monoclonal antibodies ( mAb ) , when both VLA 4 and VLA 5 on monocytes were blocked , treatment of monocytes with mAb to both LFA 1 and to Mac 1 completely inhibited monocyte migration across HSF barriers , although blocking either of these beta 2 integrins alone had no effect on migration , even when VLA 4 and VLA 5 were blocked . ^^^ This indicates that optimal beta 2 integrin dependent monocyte migration in synovial connective tissue may be mediated by either LFA 1 or Mac 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Serial frozen sections of grafts and controls were stained by immunoperoxidase for rat MAC 1 , class 2 MHC , CD 2 , CD 4 , CD 8 , B cells , VLA 4 , LFA 1 , L selectin , ICAM 1 , and VCAM 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Experiments employing blocking antibodies to adhesion molecules demonstrated requirements for the beta 1 integrin VLA 4 , beta 2 integrins LFA 1 and Mac 1 , and L selection . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Characteristics of cation binding to the 1 domains of LFA 1 and MAC 1 . ^^^ The crystal structures of the 1 domains of integrins MAC 1 ( alphaM beta 2 ; CD11b / CD18 ) and LFA 1 ( alphaL beta 2 ; CD11a / CD18 ) show that a single conserved cation binding site is present in each protein . ^^^ Here we show that the purified recombinant 1 domain of LFA 1 ( alphaLI ) binds cations , but with significantly different characteristics compared with the 1 domain of MAC 1 ( alphaMI ) . ^^^ Antagonism of LFA 1 , but not MAC 1 , mediated cell adhesion by Ca2+ may be related to the Ca2+ binding activity of the LFA 1 1 domain . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Hydrodynamic shear shows distinct roles for LFA 1 and Mac 1 in neutrophil adhesion to intercellular adhesion molecule 1 . ^^^ Both LFA 1 and Mac 1 contributed equally to adhesion efficiency over the initial 30 seconds of stimulation , but adhesion was entirely Mac 1 dependent by 120 seconds . ^^^ We conclude that at low shear , neutrophil adhesion is modulated independently through either LFA 1 or Mac 1 , which initially contribute with equal efficiency , but differ over the duration of chemotactic stimulation . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
LFA 1 , Mac 1 , CD 44 , ICAM 1 and P selectin were strongly expressed in the acute phase and to a lesser degree in the chronic phase of arthritis . ^^^ LFA 1 , Mac 1 , CD 44 , alpha 4 integrin , ICAM 1 and the selectins were found expressed on cells of the synovial infiltrate , LFA 1 , Mac 1 and ICAM 1 on the synovial lining layer , and VCAM 1 and P selectin on endothelial cells . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Commercially available anti CD 18 ( beta 2 integrin , beta chain of LFA 1 , MAC 1 , and p 150 , 95 ) and LRTC 1 MAb immunoprecipitated proteins with identical mobilities on 1 D and 2 D SDS PAGE gels . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Expression of the H 52 epitope on the beta 2 subunit is dependent on its interaction with the alpha subunits of the leukocyte integrins LFA 1 , Mac 1 and p 150 , 95 and the presence of Ca2+ . ^^^ In the presence of Ca2+ and Mg2+ , the H 52 epitope is expressed on the monomeric beta 2 subunit , the LFA 1 and Mac 1 heterodimers but not on p 150 , 95 , thus implying that this epitope is masked in p 150 , 95 . ^^^ However , expression of the H 52 epitope on Mac 1 , but not on LFA 1 , or the monomeric beta 2 subunit , is dependent on the presence of Ca2+ , thus suggesting that the chelation of Ca2+ causes a conformational change in Mac 1 which results in the loss of the epitope . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We investigated the expression of adhesion molecules in circulating neutrophils ( lymphocyte function associated antigen 1 [ LFA 1 ] , Mac 1 , and L selectin ) and endothelial cells ( soluble intercellular adhesion molecule 1 [ sICAM 1 ] ) in 23 patients with stable chronic obstructive pulmonary disease ( COPD ) , 18 subjects with exacerbated COPD , and 23 healthy volunteers . ^^^ Compared with control subjects , patients with stable COPD showed increased expression of Mac 1 ( p < 0 . 001 ) and lower levels of sICAM 1 ( p = 0 . 002 ) ; LFA 1 and L selectin expression was similar in patients and control subjects . ^^^ During exacerbations , compared with stable patients , the expression of Mac 1 and LFA 1 was reduced ( p < 0 . 001 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
At relatively low shear rates ( 100 200 s ( 1 ) ) firm adhesion is mediated in equal measure by LFA 1 binding to ICAM 3 , and Mac 1 binding to an as yet undefined ligand . ^^^ In this report we used a mouse melanoma cell line expressing an estimated 700 , 000 ICAM 1 ( CD 54 ) to examine the relative roles of LFA 1 and Mac 1 over the kinetics of heterotypic cell adhesion in shear mixed suspensions . ^^^ A direct interaction between LFA 1 and ICAM 1 was preferred over the first minute of stimulation , whereas at later times adhesion was supported equally by Mac 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Activated neutrophils aggregate in a shear field via bonding of L selectin to P selectin glycoprotein ligand 1 ( PSGL 1 ) followed by a more stable bonding of LFA 1 ( CD11a / CD18 ) to intercellular adhesion molecule 3 ( ICAM 3 ) and Mac 1 ( CD11b / CD18 ) to an unknown counter receptor . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Human PMNL migration in vitro across unstimulated human umbilical vein endothelial cells ( HUVEC ) , induced by C5a or IL 8 , was virtually all mediated by the CD 18 ( beta 2 ) integrins , LFA 1 and Mac 1 . ^^^ The integrins , LFA 1 and Mac 1 ( CD11 / CD18 ) , and VLA 4 mediated PMNL migration to arthritic joints . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Twenty atopic asthmatic ( AA ) subjects and nine controls were studied and the expression of lymphocyte function associated antigen 1 ( LFA 1 ; CD11a / CD18 ) , Mac 1 ( CD11b / CD18 ) and very late antigen 4 ( VLA 4 ; CD49d / CD29 ) on blood eosinophils was evaluated by specific monoclonal antibody ( Mab ) staining and flow cytometric analysis . ^^^ Compared with controls , eosinophils from AA showed increased expression of LFA 1 ( p < 0 . 005 ) , but not of Mac 1 or VLA 4 ( p > 0 . 1 ) . ^^^ In addition , LFA 1 expression correlated positively with blood eosinophil number ( r=0 . 792 , p < 0 . 05 ) , while no correlations were observed between Mac 1 or VLA 4 expression and blood eosinophil number . ^^^ The migration of eosinophils through human umbilical vein endothelial cells with or without anti LFA 1 , Mac 1 and VLA 4 blocking Mab was studied . ^^^ Cell migration was totally inhibited by preincubating eosinophils with anti LFA 1 ( p < 0 . 05 ) , while anti Mac 1 had no effect ( p > 0 . 1 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Increased adhesion of TMM cells was impaired neither by antibodies to VLA 4 , LFA 1 , or Mac 1 nor by antibodies to their counter receptors VCAM 1 or ICAM 1 , suggesting that adhesion molecules distinct from VCAM 1 or ICAM 1 are involved in enhanced adhesiveness of the fibroblasts to myeloid leukemic cells . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Cross linking of the LFA 1 ( CD11a / CD18 ) and MAC 1 ( CD11b / CD18 ) integrins on the surface of THP 1 monocytic cells induced the accumulation of tyrosine phosphoproteins . ^^^ In vitro kinase assays documented an increase in ERK 2 activity following both LFA 1 and MAC 1 cross linking . beta 2 Integrin cross linking also led to a marked increase in 4 h procoagulant activity ( PCA ) in THP 1 cells and purified human monocytes . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We describe a patient with clinical features compatible with a moderately severe phenotype of LAD 1 but who expresses the beta 2 integrins lymphocyte function associated molecule ( LFA ) 1 and Mac 1 at 40 % 60 % of normal levels . ^^^ A novel leukocyte adhesion deficiency caused by expressed but nonfunctional beta 2 integrins Mac 1 and LFA 1 . ^^^ This level of expression should be adequate for normal integrin function , but both the patient ' s Mac 1 on neutrophils and LFA 1 on T cells failed to bind ligands such as fibrinogen and intercellular adhesion molecule ( ICAM ) 1 , respectively , or to display a beta 2 integrin activation epitope after adhesion inducing stimuli . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Finally , in the skin both epidermal Langerhans cells ( LC ) and dermal DC are CD 8 ( ) DEC 205 ( high ) Mac 1 ( high ) , and do not express LFA 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We evaluated the relative contribution of ICAM 1 and ICAM 2 , known ligands on endothelium for LFA 1 and Mac 1 , in spontaneous neutrophil ( PMN ) transendothelial migration ( TEM ) across IL 1 activated HUVEC monolayers or TEM induced by C5a or IL 8 across unstimulated HUVEC grown on polycarbonate filters . ^^^ In contrast , with IL 1 HUVEC , mAb to ICAM 1 significantly inhibited this LFA 1 independent TEM . mAb to Mac 1 alone partially inhibited TEM and , when combined with mAb to LFA 1 , migration was almost completely blocked with all TEM conditions tested . ^^^ The contribution of alternate ligands for Mac 1 in mediating Mac 1 dependent but ICAM 1 / 2 independent C5a induced TEM was examined using anti LFA 1 treated PMN and anti ICAM treated resting HUVEC . ^^^ The results indicate that : ( 1 ) LFA 1 mediates PMN TEM primarily by interaction with ICAM 1 and ICAM 2 ; ( 2 ) ICAM 2 may function in concert with ICAM 1 in this role , especially on unstimulated endothelium , and ( 3 ) Mac 1 on PMN also plays a major role in TEM and can utilize yet to be identified ligands distinct from ICAM 1 or 2 , especially on unstimulated endothelium . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The expression of intercellular adhesion molecule 1 ( ICAM 1 ) by endothelial cells is important for the regulation of adhesion and transendothelial migration of a variety of leukocytes that express the integrins lymphocyte function associated antigen 1 ( LFA 1 ) and / or Mac 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In addition to LFA 1 , neutrophils express the beta 2 integrin Mac 1 ( CD11b / CD18 ; CR 3 ) that apparently contains multiple sites that bind invading microbes directly or through surface fixed C 3 , resulting in activation of the phagocyte function . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
To further elucidate the mechanism ( s ) involved in this process , we have used an in vitro transendothelial chemotaxis model . mAb neutralization studies performed in this system suggest that the integrins Mac 1 ( CD11b / 18 ) , VLA 4 ( alpha4beta1 ) and LFA 1 ( CD11a / 18 ) are involved in the transendothelial chemotaxis of eosinophils to eotaxin . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
These results suggest that fibrin as well as fibrinogen function as a stimulator to macrophages , and leukocyte integrin p 150 , 95 ( CD11c / CD 18 ) , not Mac 1 ( CD11b / CD18 ) , is involved in mediating fibrin stimulatory activity in macrophages . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We show that CC chemokines induced a sustained increase in monocyte adhesion to intercellular adhesion molecule 1 that was mediated by Mac 1 ( alphaMbeta 2 ) but not lymphocyte function associated antigen 1 ( LFA 1 ; alphaLbeta 2 ) . ^^^ Differential kinetics for activation of Mac 1 ( sustained ) and LFA 1 ( transient ) avidity in response to stromal derived factor 1alpha were confirmed by expression of alphaM or alphaL in alphaL deficient Jurkat cells . ^^^ Coexpressing alphaM or chimeras in mutant Jurkat cells with a `` gain of function ' ' phenotype that results in constitutively active LFA 1 demonstrated that Mac 1 was not constitutively active , whereas constitutive activity was mediated via the alphaL cytoplasmic tail , implying the presence of distinct signaling pathways for LFA 1 and Mac 1 . ^^^ Transendothelial chemotaxis of monocytes in response to MCP 1 was dependent on LFA 1 ; however , Mac 1 was involved at MCP 1 concentrations stimulating its avidity , showing differential contributions of beta 2 integrins . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
ICAM 2 , a constitutively expressed endothelial ligand for beta 2 integrins LFA 1 and Mac 1 , is involved in leukocyte adhesion to resting endothelium and in transmigration in vitro , however its role in inflammation is unclear . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Infiltration of neutrophils and expression of MAC 1 , LFA 1 , VLA 4 seem to be under control of progesterone or estradiol . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Previous findings have shown that a peptide derived from the first Ig domain of ICAM 2 ( P 1 ) binds to LFA 1 ( CD11a / CD18 ) and Mac 1 ( CD11b / CD18 ) and activates leukocyte aggregation . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Use of a photoactivatable taxol analogue to identify unique cellular targets in murine macrophages : identification of murine CD 18 as a major taxol binding protein and a role for Mac 1 in taxol induced gene expression . ^^^ This finding was supported by the concomitant failure of macrophage membranes from Mac 1 knockout mice to express immunoreactive CD 18 and the major Taxol binding protein . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In particular , attention has been drawn to the role exerted by those cytokine ( TNF alpha and IL 1 beta ) and adhesion molecules ( LFA 1 , Mac 1 , ICAM 1 ) that regulate interactions between leukocyte and endothelial cells leading to gastric microvessels occlusion and ischaemic / hypoxic endothelial epithelial cell damage . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
CONCLUSIONS : These observations indicate that exposure of HT 29 cells to bile acids or ammonia induces CD11b / CD18 ( Mac 1 ) dependent but CD11a / CD18 ( LFA 1 ) independent neutrophil adhesion to intestinal epithelial cells , and ICAM 1 is unlikely involved in the interactions . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The trafficking of leukocytes through tissues is supported by an interaction between the beta 2 ( CD 18 ) integrins CD11a / CD18 ( LFA 1 ) and CD11b / CD18 ( Mac 1 ) and their ligand ICAM 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Neutralizing Abs against the beta 2 integrins , LFA 1 and Mac 1 , decreased IL 6 production by 40 and 50 % , respectively . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Acute thermal injury of rat skin resulted in a time dependent loss of L selectin and up regulation of Mac 1 ( CD11b / CD18 ) on blood neutrophils , with no changes in LFA 1 ( CD11a / CD18 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Together these observations suggest that short exposure of HT 29 cells to TNF alpha and histamine induces CD11b / CD18 ( Mac 1 ) dependent but CD11a / CD18 ( LFA 1 ) independent neutrophil adhesion to intestinal epithelial cells , and ICAM 1 is not likely to be involved in the interactions . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
To differentiate the unique and overlapping functions of LFA 1 and Mac 1 , LFA 1 deficient mice were developed by targeted homologous recombination in embryonic stem cells , and neutrophil function was compared in vitro and in vivo with Mac 1 deficient , CD 18 deficient , and wild type mice . ^^^ After zymosan activated serum stimulation , LFA 1 deficient neutrophils demonstrated activation , evidenced by up regulation of surface Mac 1 , but did not show increased adhesion to purified ICAM 1 or endothelial cells , similar to CD 18 deficient neutrophils . ^^^ Evaluation of the strength of adhesion through LFA 1 , Mac 1 , and CD 18 indicated a marked reduction in firm attachment , with increasing shear stress in LFA 1 deficient neutrophils , similar to CD 18 deficient neutrophils , and only a modest reduction in Mac 1 deficient neutrophils . ^^^ Leukocyte influx in a subcutaneous air pouch in response to TNF alpha was reduced by 67 % and 59 % in LFA 1 and CD 18 deficient mice but increased by 198 % in Mac 1 deficient mice . ^^^ Relative contribution of LFA 1 and Mac 1 to neutrophil adhesion and migration . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The first is to allow leukocyte infiltration of respiratory mucosa , since they express LFA 1 and Mac 1 , which are ligands of ICAM 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
As evident from antibody blocking experiments , PF 4 induced adhesion involved PMN expressed L selectin as well as leukocyte function associated molecule 1 ( LFA 1 ) , whereas IL 8 involved MAC 1 . ^^^ Because blocking antibodies to LFA 1 but not to L selectin or MAC 1 abrogated PF 4 dependent marker exocytosis from PMN , the costimulatory signal provided by EC appears to be elicited through cell cell contact via LFA 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
ICAM 1 ( CD 54 ) , the ligand for LFA 1 and Mac 1 , is up regulated during inflammatory reaction on the activated vascular endothelium . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
ICAM 1 interactions with the beta 2 integrins CD11a / CD18 ( LFA 1 ) and CD11b / CD18 ( MAC 1 ) on the surface of leukocytes are important for their transendothelial migration to sites of inflammation and their function as costimulatory molecules for T cell activation . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
BACKGROUND : Lymphocyte function associate 1 ( LFA 1 ) , macrophage antigen 1 ( Mac 1 ) , and very late activation antigen 4 ( VLA 4 ) are involved in the infiltration of leukocytes into the tissues . ^^^ METHODS : We evaluated by immunocytochemistry the expression of VLA 4 , LFA 1 , and Mac 1 and their relationship with inflammatory cells and severity of disease in the induced sputum of 20 mild to moderate atopic asthmatic subjects and in 8 healthy subjects . ^^^ In contrast , LFA 1 and Mac 1 cellular expressions do not differ between asthmatic and control subjects and are not related to any specific cell type . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Adhesion molecules identified were intercellular adhesion molecule 1 ( ICAM 1 ) , ICAM 2 , ICAM 3 , vascular cell adhesion molecule 1 ( VCAM 1 ) , platelet endothelial cell adhesion molecule 1 ( PECAM 1 ) , E selectin , P selectin , L selectin , lymphocyte function associated antigen 1 ( LFA 1 ) , very late activation antigen 4 ( VLA 4 ) , Mac 1 ( CD18 / CD11b ) , and gp 150 , 95 ( CD18 / CD11c ) . ^^^ Concurrent analysis of leukocyte receptors indicated a preferential use of VLA 4 / VCAM 1 and LFA 1 / ICAM 1 at the adventitia and Mac 1 / ICAM 1 at the intima media junction . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
There is some disagreement in the literature over the respective roles of conformational changes in the 1 domain and of divalent cations ( Mg ( 2+ ) , Mn ( 2+ ) ) in the activation of LFA 1 for intercellular adhesion molecule binding . 10 ray crystallographic structures of the 1 domains of LFA 1 and Mac 1 in the presence and absence of cations show structural differences in the C terminal alpha helix ; this change was proposed to represent the active and inactive conformations of the 1 domain . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In dermal inflammation in the arthritic rats , LFA 1 accounted for most ( 69 % ) of the selectin independent monocyte migration to the chemoattractant C5a ( desArg ) ( zymosan activated serum ) , whereas LFA 1 and Mac 1 both contributed to selectin independent neutrophil recruitment to C5a ( desArg ) . alpha ( 4 ) integrin and P selectin in concert mediated monocyte recruitment to lipopolysaccharide and IFN gamma lesions ( 81 % ) . ^^^ Thus : ( 1 ) either alpha ( 4 ) or LFA 1 can mediate monocyte migration to arthritic joints in the absence of selectin function and alpha ( 4 ) together with P selectin is particularly important ; ( 2 ) LFA 1 is the predominant mechanism of selectin independent migration of neutrophils to inflamed joints ; and ( 3 ) in arthritic rats , selectin independent migration of monocytes and neutrophils to dermal inflammation is mediated by alpha ( 4 ) or LFA 1 or both LFA 1 and Mac 1 , depending on the leukocyte type , and inflammatory stimulus . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
RESULTS : Peripheral blood eosinophils constitutively expressed low levels of ICAM 1 and ICAM 2 ( < 10 FMI ) , moderate levels ( 10 50 FMI ) of CD 29 and L selectin , and high levels ( > 50 FMI ) of ICAM 3 , LFA 1 , and Mac 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
These results indicate that canine neutrophils may utilize both LFA 1 and Mac 1 to adhere to adult cardiac myocytes , with LFA 1 triggered by a PAF like activity induced in myocytes by H ( 2 ) O ( 2 ) . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In the presence of different concentrations of budesonide ( 0 . 1 100 nM ) , we tested : a ) eosinophil migration induced by C5a through HBEC monolayers ; b ) ICAM 1 expression on HBECs , stimulated with C5a and c ) LFA 1 and Mac 1 expression on eosinophils , stimulated with C5a or with ah CD 23 mabs plus GM CSF . ^^^ Preincubation of the cells with different concentrations of budesonide was also effective in down regulating the C5a induced ICAM 1 expression on HBECs and the ah CD 23 and GM CSF induced LFA 1 and Mac 1 expression on eosinophils . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Adhesion molecules LFA 1 ( CD 11a ) , Mac 1 ( CD 11b ) of t lymphocytes in bronchoalveolar lavage fluid and blood in patients with chronic respiratory tract disease ] . ^^^ The aim of our study was to assess the percentage of T lymphocytes expressing LFA 1 , Mac 1 and ICAM 1 in bronchoalveolar fluid ( BALF ) and blood of patients with sarcoidosis , atopic bronchial asthma and chronic bronchitis . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We report that treatment with TNF ( 10 ng / ml ) for 30 min resulted in a significant increase in CD 18 and MAC 1 surface expression ( P < 0 . 001 ) . ^^^ In addition , pretreatment with 15 microM SB 203580 , a p 38 MAP kinase inhibitor , for 10 min significantly inhibited TNF upregulation of CD 18 and MAC 1 ( P < 0 . 0001 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The role of LFA 1 , Mac 1 , ICAM 1 and Ia in the induction of Th 2 type of immune response in spleen during murine syngeneic pregnancy . ^^^ Immunochemical localization of ICAM 1 , LFA 1 , Mac 1 and Ia in spleen have been carried out at different stages of pregnancy and formation of cell clusters and natural cell adhesion assay with splenocytes were carried out on day 1 ( D 1 ) pregnancy and compared with control . ^^^ Upregulation of ICAM 1 , LFA 1 , Mac 1 and Ia was observed during early pregnancy . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Cell adhesion was mediated via beta ( 2 ) integrin ( LFA 1 and Mac 1 ) activation : induced adhesion to HUVECs was inhibited by blocking mAbs anti CD 18 ( 70 % 90 % ) , anti CD11a ( 50 % 60 % ) , or anti CD11b ( 60 % 70 % ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
To isolate LFA 1 / ICAM 1 interactions from Mac 1 / ICAM 1 interactions , full length chimeric ICAM 1 was developed and expressed in L cells with domains 1 and 2 from canine ICAM 1 and domains 3 5 from human ICAM 1 ( C 1 , 2 ; H 3 5 ) . ^^^ The following seven experimental conditions distinguished LFA 1 dependent H2O2 production from Mac 1 dependent production : It did not require exogenous chemotactic stimulation ; H2O2 release was more rapid , but the amount released was < 40 % of that mediated by Mac 1 adhesion ; it was inhibited by anti CD11a and anti ICAM 1 antibodies ; in contrast to that mediated by Mac 1 , it was not inhibited by anti CD11b antibody , neutrophil inhibitory factor ( NIF ) , or cytochalasin B or H 7 . ^^^ Thus , canine neutrophils seem to be able to utilize two members of the beta 2 integrin family to interact with ICAM 1 and signal H2O2 production , with LFA 1 at an early stage without prior chemotactic stimulation and Mac 1 at a later stage requiring chemotactic stimulation . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
METHODS AND RESULTS : PECAM 1 , CD 34 , ICAM 1 , VCAM 1 , VLA 4 , P and E selectin , the macrophage antigens Ki M1P and Mac 1 , and lymphocyte function antigen LFA 1 were identified immunohistochemically . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Leukocyte adhesion deficiency 1 ( LAD 1 ) is the result of mutations in the beta 2 subunit of the CD11 / CD18 integrins , LFA 1 , Mac 1 , p 150 , 95 and alphadbeta 2 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The intercellular adhesion molecule 1 ( ICAM 1 , CD 54 ) serves as a counter receptor for the beta 2 integrins , LFA 1 and Mac 1 , which are expressed on leukocytes . ^^^ This adhesion was inhibited by antibodies specific for either ICAM 1 or LFA 1 / Mac 1 , suggesting a role for these molecules in adhesion . ^^^ Nonetheless , within each cell line , blocking of ICAM 1 or LFA 1 / Mac 1 reduced LAK cell killing , suggesting that ICAM 1 is involved in mediating this killing . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Effect of integrin beta 2 subunit truncations on LFA 1 ( CD11a / CD18 ) and Mac 1 ( CD11b / CD18 ) assembly , surface expression , and function . ^^^ LFA 1 ( CD11a / CD18 ) and Mac 1 ( CD11b / CD18 ) are members of the beta 2 integrins involved in leukocyte function during immune and inflammatory responses . ^^^ We aimed to determine a minimized beta 2 subunit that forms functional LFA 1 and Mac 1 . ^^^ However , only the beta 2 variants terminating after Q 444 promote cell surface expression of LFA 1 and Mac 1 . ^^^ Thus , the major cysteine rich region and the three highly conserved cysteine residues at positions 445 , 447 , and 449 of the beta 2 subunit are not required for LFA 1 and Mac 1 surface expression . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
This enables ICAM 1s to bind leukocyte integrin receptors such as LFA 1 ( CDI1a / CD18 ) and Mac 1 ( CDI1b / CD18 ) and therefore provide adaptive changes in the adhesion process between circulating cells and the endothelium . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The number of invading macrophages was determined by immunocytochemistry for three typical macrophage antigens ( F4 / 80 , Mac 1 , LFA 1 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Flow cytometric analysis of the 3 lines revealed constitutive surface molecule expression of Mac 1 , Mac 2 , F4 / 80 , LFA 1 , B 7 1 , B 7 2 , H2Kb , H2Db , and macrophage scavenger receptor , consistent with macrophage / monocyte lineages . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
RESULTS : Patients with cirrhosis were found to have a small but statistically significant decrease in the expression of the CD 18 component of MAC 1 in N formyl methionyl leucyl phenylalanine stimulated neutrophils ( P = 0 . 04 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Recent evidence is reviewed in support of the concept that integrin ( Mac 1 and LFA 1 ) activation results from signaling that occurs through selectin binding , chemotactic factor stimulation , and , possibly , LFA 1 binding . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The effect of antihuman CD 23 Mabs on : 1 ) eosinophil migration through human umbilical vein endothelial cells ( HUVECs ) ; and 2 ) eosinophil expression of the adhesion molecules leukocyte function associated antigen 1 ( LFA 1 , CD11a / CD18 ) , macrophage antigen 1 ( Mac 1 , CD11b / CD18 ) and very late activation antigen 1 ( VLA 4 , CD49d / CD29 ) was evaluated by specific Mab staining and flow cytometric analysis . ^^^ As compared to controls , freshly isolated eosinophils from atopic individuals showed enhanced migration through HUVECs ( p < 0 . 05 ) and increased LFA 1 expression ( p < 0 . 01 ) , but similar Mac 1 and VLA 4 expression ( p > 0 . 1 for both ) . ^^^ Similarly , incubation of the cells with antihuman CD 23 Mabs induced dose dependent upregulation of LFA 1 and Mac 1 expression , whereas no changes in VLA 4 expression were observed ( p > 0 . 1 ) . ^^^ Finally , the enhanced eosinophil migration induced by antihuman CD 23 Mab stimulation was significantly inhibited by antihuman LFA 1 ( 84+ / 14 % ( mean+ / SEM ) ; p < 0 . 01 ) and VLA 4 Mabs ( 47+ / 15 % ; p < 0 . 05 ) but not by antihuman Mac 1 Mabs ( p > 0 . 1 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
OBJECTIVE : To study the expression of L selectin , Mac 1 , LFA 1 on CML progenitor cells in relation to CML progression and therapeutic effect . ^^^ METHODS : The expression of adhesion molecules ( LFA 1 , Mac 1 , L selectin ) on bone marrow CD34+ cells from 34 CML patients were analyzed by three color flow cytometry . ^^^ IFN alpha and allo BMT restore the expression of Mac 1 , L selectin and LFA 1 to normal on CML CD34+ cells . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The use of lymphocyte function associated antigen ( LFA ) 1 deficient mice to determine the role of LFA 1 , Mac 1 , and alpha 4 integrin in the inflammatory response of neutrophils . ^^^ Here we use LFA 1 ( / ) mice , function blocking monoclonal antibodies , and intravital microscopy to investigate the roles of LFA 1 , Mac 1 , and alpha 4 integrins in neutrophil recruitment in vivo . ^^^ Blocking Mac 1 in the presence of LFA 1 significantly reduces emigration , suggesting cooperation between these two integrins . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We found that antibodies against VCAM 1 ( CD 106 ) , VLA 4 ( CD49d / CD29 ) , ICAM 1 ( CD 54 ) , and LFA 1 ( CD11a / CD18 ) did not influence the increased number of antigen specific T cells in the CSF However , upon intravenous ( i . v . ) injection , anti PECAM 1 ( CD 31 ) antibody or PECAM Ig chimeric molecule inhibited the trafficking of alpha / beta TCR+ Vbeta3+ Mac 1 cells into the CNS . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Blood samples taken 24 h preoperatively from 49 patients ( 3 18 years old ) were stained with monoclonal antibodies for adhesion molecules ( ICAM 1 , LFA 1 , Mac 1 ) or constitutive / activation markers ( CD 4 , CD 14 , CD 16 , CD 25 , CD 54 , CD 69 , HLA DR ) and measured on a microbead calibrated FCM . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The cecal peritoneum 24 hr after perforation was stained with specific antibodies against LFA 1 , Mac 1 , VLA 4 , ICAM 1 , VCAM 1 , and fibronectin diluted with cold University of Wisconsin ( UW ) solution in conjunction with immuno gold labeling . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Effects of IL 8 , Gro alpha , and LTB ( 4 ) on the adhesive kinetics of LFA 1 and Mac 1 on human neutrophils . ^^^ LFA 1 ( CD11a / CD18 ) appears to be more important than Mac 1 ( CD11b / CD18 ) in neutrophil emigration at inflammatory sites , but little is known of the relative binding characteristics of these two integrins under conditions thought to regulate firm adhesion . ^^^ The present study examined the effect of chemoattractants on the kinetics of LFA 1 and Mac 1 adhesion in human neutrophils . ^^^ Using well characterized monoclonal antibodies , we showed that activation of LFA 1 and Mac 1 by Gro alpha was completely blocked by anti CXC chemokine receptor R 2 , but activation of these integrins by interleukin 8 was most effectively blocked by anti CXC chemokine receptor R 1 . ^^^ The topographical distribution of beads also reflected significant differences between LFA 1 and Mac 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In a purified system , HK and HKa inhibited the binding of soluble fibrinogen and ICAM 1 to immobilized Mac 1 , but not the binding of ICAM 1 to immobilized LFA 1 ( CD11a / CD18 , alphaLbeta 2 ) . ^^^ Accordingly , HK , HKa , and domain 5 inhibited the adhesion of Mac 1 but not LFA 1 transfected K 562 human erythroleukemic cells to ICAM 1 . ^^^ Taken together , HKa ( and particularly domain 5 ) specifically interacts with Mac 1 but not with LFA 1 , thereby blocking Mac 1 dependent leukocyte adhesion to fibrinogen and endothelial cells in vitro and in vivo and serving as a novel endogenous regulator of leukocyte recruitment into the inflamed tissue . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Subsequent challenge with LPS i . p . was associated with upregulation of Mac 1 and ICAM 1 on PMN , but not of VLA 4 or LFA 1 ( FACS analysis ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Data are now presented to show that , in addition to the LFA 1 and other integrin mediated leucocyte adhesions , cistifolin inhibits the Mac 1 ( CD11b / CD18 ) dependent monocyte adhesion to fibrinogen in a concentration dependent manner . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The average rolling velocities in venules were elevated in LFA 1 ( / ) mice ( 11 . 0 + / 0 . 7 microm / s ) and Mac 1 ( / ) mice ( 10 . 1 + / 1 . 1 microm / s ) compared to wild type mice ( 4 . 8 + / 0 . 3 microm / s ; P < . 05 ) , but were lower than in CD 18 ( / ) mice ( 28 . 5 + / 2 . 1 microm / s ) . ^^^ When both LFA 1 and Mac 1 were absent or blocked , rolling velocity became dependent on shear rate and approached that of CD 18 ( / ) mice . ^^^ In addition , leukocyte adhesion efficiency was decreased in LFA 1 ( / ) mice to near CD 18 ( / ) levels , but decreased only slightly in Mac 1 ( / ) mice . ^^^ Control of leukocyte rolling velocity in TNF alpha induced inflammation by LFA 1 and Mac 1 . ^^^ In this study , mice that are deficient for either one of the beta ( 2 ) integrins , alpha ( L ) beta ( 2 ) ( LFA 1 ) or alpha ( M ) beta ( 2 ) ( Mac 1 ) , were used to determine which of the beta ( 2 ) integrins are responsible for slowing rolling leukocytes . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
TNF alpha stimulation increased the occurrence of adhesion molecules for neutrophil surface , lymphocyte function associated antigen 1 ( LFA 1 ) , and macrophage antigen 1 ( Mac 1 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule 1 ( ICAM 1 ) was up regulated by p 43 and mediated p 43 induced cell cell adhesion via the interaction with LFA 1 or Mac 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
OBJECTIVE : The aim of this study was to analyze mutations in DNA from patients with leukocyte adhesion deficiency ( LAD ) , an immunodeficiency caused by absence of the beta ( 2 ) subunit ( CD 18 ) of the leukocyte integrins LFA 1 ( CD11a / CD18 ) , Mac 1 ( CD11b / CD18 ) , p 150 , 95 ( CD11c / CD18 ) , and CR 4 ( CD11d / CD18 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
This was accompanied by a rapid redistribution of active LFA 1 , but not Mac 1 , into membrane patches , a necessary component for optimum adhesion efficiency . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
MATERIAL / METHODS : 140 EMBs from dilated cardiomyopathy ( DCM ) patients and 14 autopsy heart samples ( controls ) were immunostained for T lymphocytes ( CD 2 , CD 3 , CD 4 , CD 8 ) , beta ( 2 ) integrin+ infiltrates ( CD 18 , LFA 1 , Mac 1 ) and CAMs ( immunoglobulin superfamily : ICAM 1 , HLA class 1 , HLA DR , VCAM 1 , CD 58 ; selectins : CD62E and CD62P ; and the beta ( 1 ) integrin chain CD 29 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Inhibitory effect of olopatadine on antigen induced eosinophil infiltration and the LFA 1 and Mac 1 expression in eosinophils . ^^^ Olopatadine dose dependently repressed the IL 5 induced expressions of CD11a / CD18 ( LFA 1 ) and CD11b / CD18 ( Mac 1 ) on rat peritoneal eosinophils . ^^^ These results suggest that olopatadine may inhibit antigen induced eosinophil infiltration through repression of LFA 1 and Mac 1 expression . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Monocyte and neutrophil expression of adhesion molecules Mac 1 , LFA 1 and 3 , ICAM 1 , and VLA 4 were measured at 0 , 120 , and 240 minutes by using laser flow cytometry . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Histomorphological evaluation of the vessels was performed , and endothelial expression of ICAM 1 , VCAM 1 , and E selectin was also evaluated , as well as distribution of LFA 1 , Mac 1 , VLA 4 , RM3 / 1 , 27E10 , and 25F9 bearing cells in the subepithelial tissue . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
This heterophilic interaction was specifically attributed to a direct interaction of JAM 3 with the beta 2 integrin Mac 1 and to a lower extent with p150 . 95 ( alphaXbeta 2 , CD11c / CD18 ) but not with LFA 1 ( alphaLbeta 2 , CD11a / CD18 ) or with beta 1 integrins . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
METHODS : Blood eosinophils were isolated from seven atopic asthmatic patients and seven control subjects and the `` at baseline ' ' expression of lymphocyte function associated antigen 1 ( LFA 1 ) , macrophage antigen 1 ( Mac 1 ) and very late antigen 4 ( VLA 4 ) was assessed by monoclonal antibody staining and flow cytometry analysis . ^^^ Asthmatic patients underwent allergen challenge and the expression of LFA 1 , Mac 1 and VLA 4 by blood eosinophils was again evaluated 3 h and 24 h after allergen challenge . ^^^ RESULTS : As compared to controls , eosinophils from atopics showed at baseline enhanced LFA 1 expression ( P=0 . 0012 ) , but similar Mac 1 or VLA 4 expression ( P > 0 . 1 , each comparison ) . ^^^ Allergen challenge was also followed by a significant decrease in LFA 1 expression by eosinophils , at 3 h ( P=0 . 002 ) and at 24 h ( P=0 . 038 ) , while no changes in Mac 1 and VLA 4 were observed . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The expression of the following cell adhesion molecules , their beta 1 and beta 2 integrin ligands and the cytokine tumour necrosis factor alpha ( TNF alpha ) was investigated by light and electron microscope immunohistochemistry in the liver tissue in 20 patients with colorectal and gastric cancer also presenting with liver metastases : intercellular adhesion molecule 1 ( ICAM 1 ) , vascular endothelial adhesion molecule 1 ( VCAM 1 ) , E selectin , leucocyte function associated antigen 1 ( LFA 1 ) , macrophage antigen 1 ( Mac 1 ) , and very late antigen 4 ( VLA 4 ) . ^^^ Tumour infiltrating host cells sowing positive immunoreactivity for ICAM 1 , VCAM 1 , LFA 1 , Mac 1 , and VLA 4 were located mainly at the boundary between liver parenchyma and the metastasis . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Finally , end target chemoattractant responses were predominantly Mac 1 dependent , whereas nondominant chemoattractants used primarily LFA 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Expression of uPAR correlated with activation of beta ( 2 ) integrins lymphocyte function associated antigen 1 ( LFA 1 ) and macrophage antigen 1 ( Mac 1 ) , measured by using monoclonal antibodies ( mAbs ) 24 and CBRM1 / 5 . ^^^ Excessive adhesion of MNCs to HUVECs was inhibited by mAbs anti CD 18 ( 84 % ) , anti CD11a ( 51 % ) , and anti CD11b ( 57 % ) , indicating a major contribution of LFA 1 and Mac 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
To gain a profound insight into the underlying mechanisms , we phenotypically characterized pan leukocytes ( CD 18 ) , naive T lymphocytes ( CD 3 , CD 2 , CD 4 , CD 8 ) , activated lymphocytes ( LFA 1 , LFA 3 , VLA 4 , ICAM 1 , CD 69 , CD45R0 ) , macrophages ( Mac 1 , 27E10 ) , B lymphocytes ( CD 19 ) and NK cells ( CD 57 ) in DCM and control hearts . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
RESULTS : Specific cation dependent binding of lymphocytes to bronchial endothelium was observed which was significantly inhibited by antibodies against P selectin , PSGL 1 , L selectin , LFA 1 , ICAM 1 and ICAM 2 but not E selectin , VLA 4 , VCAM 1 or Mac 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Leukocyte integrins LFA 1 , Mac 1 , and VLA 4 were preferentially localized to microvilli and ruffles of macrophages and lymphocytes . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
This entry is facilitated by the up regulation of VCAM 1 and ICAM 1 on HBMECs and a corresponding increase of LFA 1 , VLA 4 , and MAC 1 on monocytes . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Monocytes migrate in the first 2 hours of inflammation making use of alpha4beta1 but not of Mac 1 or lymphocyte function associated antigen 1 ( LFA 1 ) integrins . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
IL 8 and T lymphocytes expressing adhesion molecules LFA 1 ( CD11a / CD18 ) , Mac 1 ( CD11b / CD18 ) Lsel in lower respiratory tract obstructive disease ] . ^^^ This study evaluated the concentration of IL 8 and count of T lymphocytes expressing adhesion molecules LFA 1 , Mac 1 , Lsel and their correlation in patients with asthma and COPD in periods of exacerbation and clinical improvement ( after seven days of anti inflammatory treatment ) . ^^^ The significant correlation observed in COPD patients between IL 8 concentration and counts of T cells expressing LFA 1 ( r = 0 . 44 ) , Mac 1 ( r = 0 . 49 ) , Lsel ( r = 0 . 42 ) in exacerbation period suggest a chemotactic influence of IL 8 on T lymphocytes . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Expression of the integrins LFA 1 , VLA 4 and Mac 1 and of members of the immunoglobulin superfamily of adhesion molecules ICAM 1 and VCAM 1 was suppressed in the central nervous system ( CNS ) following rEPF treatment . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In contrast , levels of beta ( 2 ) integrins ( e . g . , LFA 1 and Mac 1 ) , the molecules that would be necessary for C 8161 stable adhesion to the endothelium substrate , were found to be very low on these melanoma cells . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In contrast , Mac 1 was not up regulated and plasma transaminase activities and the area of necrosis ( 21 + / 9 % ) were significantly reduced in CD 18 deficient animals . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Platelet activating factor ( PAF ) induced neutrophil lung sequestration may require cell surface adhesion molecules ( macrophage 1 antigen ( MAC 1 ) and lymphocyte function associated antigen 1 ( LFA 1 ) ) . ^^^ MAC 1 and LFA 1 expression were assessed by immunocytochemistry , and leukotriene B 4 ( LTB 4 ) was measured by enzyme immunoassay in induced sputum supernatants . ^^^ Compared with baseline and L PAF , PAF decreased the percentages of MAC 1 and LFA 1 positive neutrophils in PB at 5 min , but increased the percentages of MAC 1 and LFA 1 in neutrophil induced sputum . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
It was concluded that ( 1 ) the portion of the CD 18 stalk region deleted in mut 1 is critical for beta ( 2 ) integrin heterodimer expression but the portion of the cytoplasmic domain truncated in mut 2 is not ; and ( 2 ) the mut 2 cytoplasmic domain truncation impairs binding function of LFA 1 but not of Mac 1 . ^^^ Lymphocyte function associated antigen 1 ( LFA 1 ) and macrophage antigen 1 ( Mac 1 ) containing the mut 1 beta ( 2 ) subunit were expressed at very low levels compared with wild type ( wt ) beta ( 2 ) . ^^^ Mac 1 and LFA 1 expression with the mut 2 beta ( 2 ) subunit were equivalent to results with wt beta ( 2 ) . ^^^ Studies with the patient ' s neutrophils ( PMNs ) were consistent with functional impairment of LFA 1 but not of Mac 1 . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In addition , the level of ICAM 1 expression on sinusoidal endothelial cells greatly increased from 8 h after exposure to endotoxin , and these cells were adhered to neutrophils that expressed both LFA 1 and Mac 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Lymphocyte function associated antigen 1 ( LFA 1 ) , macrophage 1 molecule ( Mac 1 ) and very late appearing antigen 4 ( VLA 4 ) , all ligands of ICAM 1 and VCAM 1 , were present on the transmigrated neutrophils and macrophages . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In vitro , RAGE dependent leukocyte adhesion to endothelial cells was mediated by a direct interaction of RAGE with the beta 2 integrin Mac 1 and , to a lower extent , with p 150 , 95 but not with LFA 1 or with beta 1 integrins . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Mac 1 , but not LFA 1 , uses intercellular adhesion molecule 1 to mediate slow leukocyte rolling in TNF alpha induced inflammation . ^^^ We have previously shown that Mac 1 and LFA 1 play a cooperative role in slow leukocyte rolling in inflamed vessels , and that , although both have a role in leukocyte adhesion , the contribution from LFA 1 exceeds that of Mac 1 . ^^^ In this study , we used mice deficient in ICAM 1 ( ICAM 1 ( null ) ) to study the function of ICAM 1 as an endothelial ligand for Mac 1 and LFA 1 . ^^^ Similarly , leukocyte adhesion efficiency in ICAM 1 ( null ) mice ( 0 . 11 + / 0 . 01 mm ) was similar to that in Mac 1 ( / ) ( 0 . 12 + / 0 . 03 mm ) mice but significantly increased compared with that in LFA 1 ( / ) ( 0 . 08 + / 0 . 01 mm ) mice and significantly reduced from that in wild type ( 0 . 26 + / 0 . 04 mm ) . ^^^ We conclude that endothelial ICAM 1 is the main ligand responsible for slow leukocyte rolling mediated by Mac 1 , but not LFA 1 . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
JAMs associate through their extracellular domains with the leukocyte beta 2 integrins LFA 1 and Mac 1 as well as with the beta 1 integrin alpha4beta1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We examined the expression and dynamics of interleukin 1beta ( IL 1beta ) , tumor necrosis factor alpha ( TNF alpha ) , monocyte chemotactic protein 1 ( MCP 1 ) , macrophage inflammatory protein 1alpha ( MIP 1alpha ) , MIP 2 , GRO / cytokine induced neutrophil chemoattractant 2alpha ( CINC 2alpha ) , intercellular adhesion molecule 1 ( ICAM 1 ) , leukocyte function associated antigen 1 ( LFA 1 ; CD11a / CD18 ) , and Mac 1 ( CD11b / CD18 ) in rat chronic reflux esophagitis . ^^^ Localization of adhesion molecules and cytokines was investigated by immunohistochemical staining , and numbers of LFA 1 or Mac 1 positive cells were quantified . ^^^ Numbers of LFA 1 and Mac 1 positive cells were significantly increased in rat chronic esophagitis . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
ICAM 1 on endothelial cells mediates leukocyte adhesion by binding to leukocyte function associated antigen 1 ( LFA 1 ) and macrophage antigen 1 ( Mac 1 ) . ^^^ Recombinant mouse sICAM 1 induces the production of macrophage inflammatory protein 2 ( MIP 2 ) in mouse astrocytes by a novel LFA 1 and Mac 1 independent mechanism . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Loss of LFA 1 , but not Mac 1 , protects MRL / MpJ Fas ( lpr ) mice from autoimmune disease . ^^^ Mice deficient in LFA 1 , but not Mac 1 , showed significantly increased survival , decreased anti DNA autoantibody formation , and reduced glomerulonephritis . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Coincubation of eosinophils with integrin blocking monoclonal antibodies in adhesion assays showed that an association of the VLA 4 , LFA 1 , and Mac 1 integrins mediate the adhesion of SCA eosinophils to FN . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Contributions of LFA 1 and Mac 1 to brain injury and microvascular dysfunction induced by transient middle cerebral artery occlusion . ^^^ The objective of this study was to determine whether and how genetic deficiency of either lymphocyte function associated antigen 1 ( LFA 1 ) or macrophage 1 ( Mac 1 ) alters the blood cell endothelial cell interactions , tissue injury , and organ dysfunction in the mouse brain exposed to focal I / R . ^^^ Middle cerebral artery occlusion was induced for 1 h ( followed by either 4 or 24 h of reperfusion ) in wild type mice and in mice with null mutations for either LFA 1 or Mac 1 . ^^^ Mice with null mutations for LFA 1 or Mac 1 exhibited significant reductions in infarct volume . ^^^ However , after 24 h of reperfusion , leukocyte adhesion was reduced in both LFA 1 and Mac 1 deficient mice compared with their wild type counterparts . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The increase in costimulatory ( CD 80 and CD 86 ) and adhesion ( ICAM 1 , PGP 1 , LFA 1 , and Mac 1 ) in peripheral blood mononuclear cells was also attenuated by food restriction and to a lesser extent by fish oil alone . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
In particular , a specific interaction between kringle 4 and alphaMbeta 2 integrin ( Mac 1 ) but not leukocyte function antigen 1 ( LFA 1 ) was identified . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Our purpose in this study was to examine the levels of soluble intracellular adhesion molecule 1 ( sICAM 1 ) and Mac 1 ( CD11b / CD18 ) , lymphocyte function associated antigen 1 ( LFA 1 ) in both neutrophils and lymphocytes in stable patients with COPD and in the healthy control groups consisting of non smokers , and in smokers without COPD and also to evaluate the relationship between the parameters related to the severity of the disease . ^^^ Flow cytometry was used for Mac 1 and LFA 1 levels . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
To investigate mechanisms that may underlie the non progression that occurs in immunodeficiency virus infected chimpanzees , we amplified the protein coding regions of multiple non human primate ICAMs 1 5 and two ICAM ligands , leukocyte function associated antigen 1 ( LFA 1 ) and macrophage antigen 1 ( Mac 1 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Lymphocyte function associated molecule 1 ( LFA 1 ; CD11a / CD18 ) influenced the capture phase of PMN binding to both melanoma cells and the endothelium , whereas Mac 1 ( CD11b / CD18 ) affected prolonged PMN melanoma aggregation . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
We evaluated the inflammatory response and the expression of cellular adhesion molecules ( ICAM 1 , VCAM 1 , CD 18 , LFA 1 and Mac 1 ) in lungs of BALB / c mice infected with Paracoccidioides brasiliensis conidia . ^^^ CD 18 and Mac 1 but not LFA 1 were expressed with a high intensity on neutrophils and macrophages present in the inflammatory infiltrate . ^^^ These findings suggest that during these early stages , up regulation of ICAM 1 , VCAM 1 , CD 18 and Mac 1 expression occurs , participating in the inflammatory process and as such , in the pathogenesis of paracoccidioidomycosis ( PCM ) . . ^^^ CD 18 and Mac 1 but not LFA 1 were expressed with a high intensity on neutrophils and macrophages present in the inflammatory infiltrate . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Leukocyte cell adhesion molecules ( LFA 1 , Mac 1 and VLA 4 ) mobilization in circulating granulocytes were analysed by flow cytometry and endothelial cell adhesion molecules ( ICAM 1 and VCAM 1 ) were detected by immunohistochemistry on lung sections . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The results in this study indicate that although LktA can efficiently bind to the CD 18 subunit of both LFA 1 and Mac 1 , post binding signaling events including elevation of intracellular calcium and CD 18 tail phosphorylation are only observed through LFA 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Beta 2 integrins / ICAM 1 adhesion mechanisms were examined and the results indicate LFA 1 and Mac 1 cooperate to mediate the PMN EC melanoma interactions under shear conditions . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The expression of the following cell adhesion molecules and their beta 1 and beta 2 integrin ligands was investigated in the liver tissue from 3 patients with non bacillar peliosis using light and electron microscope immunohistochemistry : intercellular adhesion molecule 1 ( ICAM 1 ) , vascular cell adhesion molecule 1 ( VCAM 1 ) , E selectin , platelet endothelial cell adhesion molecule 1 ( PECAM 1 ) , leukocyte function associated antigen 1 ( LFA 1 ) , macrophage antigen 1 ( Mac 1 ) , and very late antigen 4 ( VLA 4 ) . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Differential activation of LFA 1 and Mac 1 ligand binding domains . ^^^ Mutation of an invariant isoleucine residue in the 1 domains of alphaL and alphaM has previously been reported to activate LFA 1 and Mac 1 , respectively . ^^^ We find that mutation of the isoleucine residue in the proposed `` socket for isoleucine ' ' in full length alphaL does not lead to an active LFA 1 , although mutation of the equivalent residue in alphaM does convey constitutive activity to Mac 1 . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Fundamentally different roles for LFA 1 , Mac 1 and alpha 4 integrin in neutrophil chemotaxis . ^^^ Although the LFA 1 , Mac 1 and alpha ( 4 ) integrins are required for chemotaxis , it is unknown how they are regulated or what specific role they play . ^^^ Here we show that these chemoattractants also activate and use Mac 1 and LFA 1 in a differential manner during chemotaxis . ^^^ Using integrin specific substrata , we demonstrate that cell movement in response to IL 8 is mediated by Mac 1 , whereas LFA 1 is required for directional migration . ^^^ By contrast , chemotaxis to fMLP requires Mac 1 for cell movement , whereas LFA 1 and alpha ( 4 ) integrin are required for directional migration . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Treating mice with antibodies to the beta 2 integrins LFA 1 and Mac 1 showed that LFA 1 was primarily responsible for mediating rolling on ICAM 1 in this model . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
LFA 1 ( CD11a / CD18 integrin ) influenced the capture phase of PMN binding to both melanoma cells and the endothelium , while Mac 1 ( CD11b / CD18 integrin ) affected prolonged PMN melanoma aggregation . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Bone marrow from CD 18 / ( MAC 1 / ) homozygous deletion recombinant negative mice demonstrates increased longevity in long term bone marrow culture and decreased contribution to irradiation pulmonary damage . ^^^ BACKGROUND : Bone marrow macrophage surface expression of CD 18 ( MAC 1 , LFA 1 ) is involved in cellular binding to 5 CAM 1 and 5 CAM 2 adhesion molecules expressed on endothelial cells . ^^^ CONCLUSION : The data demonstrate a complex role of CD 18 ( MAC 1 ) in macrophage progenitor and macrophage cellular interaction involving stromal cells of the bone marrow and lung . . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Antibodies to HK receptors on leukocytes including Mac 1 , LFA 1 , uPAR , and C1qR inhibited IL 1beta secretion induced by tKa 98 % , 89 % , 85 % , and 62 % , respectively . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
The dissociation rates were calculated under low force conditions for ICAM 1 interactions with both beta 2 integrins , LFA 1 ( CD11a / CD18 ) and Mac 1 ( CD11b / CD18 ) , together and separately by using functional blocking antibodies on PMNs . ^^^
Interacting proteins: P11215 and P05107	Pubmed	SVM Score :0.0
Here , in a model of thrombohemorrhagic vasculitis , the CD 18 integrin Mac 1 on neutrophils recognized complement C 3 deposited within vessel walls and triggered a signaling pathway involving the Src family kinase Hck and the Syk tyrosine kinase . ^^^