Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.53837506
ICAM 1 serves as a specific ligand for lymphocyte function associated antigen 1 ( LFA 1 ) , a cell surface protein expressed on all leukocytes . 0.53837506^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :1.091573
The LFA 1 interaction with its ligand ICAM 1 mediates not only cell adhesion but also signal transduction in immunologic and inflammatory cell responses . 1.091573^^^ ICAM 1 forms a receptor ligand pair with LFA 1 and is involved in a number of significant cellular interactions , e . g . in providing dynamic position specific information to guide lymphocyte and leukocyte localization in the immune response . 0.79943481^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.86711002
These results indicate that the interactions between LFA 1 and ICAM 1 play an important role in mediating the suppressive activity of anti CD 3 activated CD4+ T cells to B cells . 0.86711002^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.73040643
Since cytoadherence to postcapillary venular endothelium is thought to be a major factor in the virulence of P . falciparum malaria , we have examined the interaction between ICAM 1 and the P . falciparum infected cell , and have compared it with the interaction to the physiological counter receptor , the leukocyte integrin LFA 1 . 0.73040643^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.77945906
The interaction between LFA 1 and its natural ligand , ICAM 1 , plays an important role in leukocyte adhesion and signal transduction . 0.77945906^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.89747257
These results indicate that the interactions between LFA 1 and ICAM 1 play an important role in mediating the collaboration between activated CD4+ T cells and B cells necessary for the induction of B cell proliferation and differentiation , and for enhancement of IL 2 production by CD4+ T cells . 0.89747257^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.84503738
Because cellular and purified Mac 1 interact with cellular and purified ICAM 1 , we conclude that ICAM 1 is a counter receptor for Mac 1 and that this receptor pair is responsible , in part , for the adhesion between stimulated neutrophils and stimulated endothelial cells . . 0.84503738^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.54235122
These data suggest that ICAM 1 exists in its native membrane bound and shed form as a non covalent dimer , and that dimerization directly correlates with enhanced binding to LFA 1 . . 0.54235122^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.66056212
This suggests that , in addition to the inside out signaling , a previously unrecognized requirement for full activation of the leukocyte integrin LFA 1 is the initial interaction with its ligand ICAM 1 . 0.66056212^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.9479544
We have analyzed the influence of non antigen specific interactions between ICAM 1 and LFA 1 in target recognition by allospecific and antigen specific T cells at the clonal level , using human and mouse fibroblasts transfected with HLA DR 1 or DR 2 with or without co expression of ICAM 1 , as antigen presenting cells . 0.9479544^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.96014978
We conclude that interactions between ICAM 1 and LFA 1 on epidermal LC and LFA 1 on spleen DC with their respective ligands on our Th 1 clones are required for optimal presentation of protein Ag to Th 1 . 0.96014978^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.84944886
The affinity was found to be too low for direct measurements , requiring instead the use of competition protocols in which ICAM 1 competes for binding with radiolabeled Fab from a monoclonal antibody specific for LFA 1 . 0.84944886^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.53019458
This demonstrates the LFA 1 is not activated , as was confirmed by low adhesion to ICAM 1 . 0.53019458^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.71822436
By using a single round infection assay based on an ICAM 1 negative target T cell line stably transfected with an HIV 1 long terminal repeat driven luciferase gene construct , we have been able to demonstrate that the acquisition of host derived ICAM 1 by HIV 1 has functional significance , since it leads to a pronounced increase in viral infectivity ( 4 . 6 to 9 . 8 fold ) in an ICAM 1 / LFA 1 dependent fashion , as shown by blocking with anti ICAM 1 and LFA 1 antibodies . 0.71822436^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.68123066
It may instead have blocked the direct interaction between LFA 1 and ICAM 1 thus preventing the damage produced by the respiratory burst of neutrophils . 0.68123066^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.56105161
Clones expressing similar levels of cell surface LFA 1 were tested for their ability to bind to immobilized ICAM 1 . 0.56105161^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.71428639
The interactions between ICAM 1 and CD 18 appeared to be important in the adhesion and the migration of PMNs to SECs . 0.71428639^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.7446555
This effect was mediated via the interaction between LFA 1 and ICAM 1 which was demonstrated by the fact that specific monoclonal antibodies against these cell adhesion molecules could block the effect of the AGEs . 0.7446555^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.50746202
Our results led us to postulate that the strong interaction taking place between virally incorporated ICAM 1 and cell surface activated LFA 1 markedly enhances the efficiency of virus binding and entry , thus favoring greater infection by ICAM 1 bearing HIV 1 particles . 0.50746202^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :1.5112302
A soluble form of LFA 1 ( sLFA 1 ) , normally expressed as two noncovalently associated membrane bound subunits , has been produced , and its interaction with ICAM 1 has been examined . 1.5112302^^^ Interaction of LFA 1 with ICAM 1 is important in a number of cellular events , including Ag specific T cell activation and leukocyte transendothelial migration , which are known to be typically transient and highly regulated . 0.5727195^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.77875962
A direct interaction between LFA 1 and ICAM 1 was preferred over the first minute of stimulation , whereas at later times adhesion was supported equally by Mac 1 . 0.77875962^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.7253298
Increased interaction of LFA 1 with ICAM 1 was further confirmed by enhanced adhesion of CD 82 overexpressing Jurkat cells to immobilized ICAM 1 Ig . 0.7253298^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :1.4300783
This is in contrast to neutrophil adhesion to ICAM 1 transfected cells , where LFA 1 binds with a higher avidity than Mac 1 to ICAM 1 . 3 ) Following stimulation , the capacity of LFA 1 to support aggregate formation decreases with time at a rate approximately 3 fold faster than that of Mac 1 . 1.4300783^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.648163
The adhesion probabilities of this pair of cells were mediated by specific interactions between ICAM 1 and LFA 1 . 0.648163^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.58798625
The aim of this work was to study comparatively the adhesive interaction between LFA 1 and ICAM 1 by a micropipette technique and a flow chamber method , and also to explore the effects of tumor necrosis factor ( TNF alpha ) , phytohemagglutinin ( PHA ) , and tetramethylpyrazine ( TMP ) on this interaction . 0.58798625^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.58591405
The induction of MMP 10 was found to be dependent on the specific interaction between LFA 1 and ICAM 1 , which play a central role in regulating the expression of genes involved in the rate limiting steps of lymphoma development . 0.58591405^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.60571859
The recognition and killing of prostate cancer cells occurs in a gammadelta T cell receptor dependent manner and it also appears to involve interactions between ICAM 1 and CD 18 . 0.60571859^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.64314315
Recent studies identified ICAM 1 as a co stimulatory ligand that binds to lymphocyte function associated antigen 1 ( LFA 1 ) , thereby promoting the activation of T cells . 0.64314315^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.55995483
High affinity LFA 1 dissociated 10 fold faster when bound to monomeric compared with dimeric ICAM 1 , corresponding to bond lifetimes of 25 and 330 s , respectively . 0.55995483^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.8543844
Interactions between intercellular adhesion molecule 1 ( ICAM 1 , CD 54 ) and leukocyte function associated antigen 1 ( LFA 1 , CD11a / CD18 ) play a critical role in T cell B cell collaboration . 0.8543844^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.83643813
Intercellular adhesion molecule 1 ( ICAM 1 ) has been shown to interact with the integrin leukocyte function associated antigen 1 ( LFA 1 ) in a variety of cell cell adhesion phenomena . 0.83643813^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.87441105
We have tested the hypothesis that LFA 1 interaction with its ligand intercellular adhesion molecule 1 ( CD 54 ) ( ICAM 1 ) is such a costimulatory interaction in a model system using biochemically purified ICAM 1 and TCR cross linking by anti CD 3 mAb OKT 3 immobilized on plastic . 0.87441105^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.59147445
A binding interface on the 1 domain of lymphocyte function associated antigen 1 ( LFA 1 ) required for specific interaction with intercellular adhesion molecule 1 ( ICAM 1 ) . 0.59147445^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.51855919
Interaction between intercellular adhesion molecule 1 ( ICAM 1 ) and lymphocyte function associated antigen 1 ( LFA 1 ) might be involved in the pathogenesis of liver diseases . 0.51855919^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.81841238
BACKGROUND : The interaction between intercellular adhesion molecule 1 ( ICAM 1 ) and its ligand , leukocyte function associated antigen 1 ( LFA 1 ) , is especially relevant in allograft rejection . 0.81841238^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.87175135
We show that on human T cells , the interaction of the beta 2 integrin leucocyte function associated antigen 1 ( LFA 1 ) with its ligand intercellular adhesion molecule 1 ( ICAM 1 ) will decrease adhesion mediated by alpha4beta1 and , to a lesser extent , alpha5beta1 . 0.87175135^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.75690502
Our results demonstrate that interaction of the beta 2 integrin LFA 1 with its ligand intercellular adhesion molecule 1 induces remodeling of T lymphocyte morphology and activation and redistribution of the cytoplasmic tyrosine kinases FAK and PYK 2 . . 0.75690502^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.63544273
By binding to CD11a the alpha subunit of LFA 1 LFA 1 is prevented from binding with its ligand , intercellular adhesion molecule 1 ( ICAM 1 ) . 0.63544273^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.57416262
Fewer resting cord blood T cells expressed CD 54 , the ligand for LFA 1 . 0.57416262^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Here we analyse the roles of HUVEC adhesion molecules VCAM 1 , ICAM 1 and E selectin , and the counter receptors VLA 4 , LFA 1 and SLex in the increase of LGL / NK adhesion to HUVEC , stimulated with Salmonella Minnesota mR 595 bacteria , LPS or TNF alpha . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
It was found that the MoAbs against LFA 1 ( CD11a ) , CD11c , LFA 3 ( CD 58 ) , ICAM 1 ( CD 54 ) or HLA DR could significantly suppress T cell proliferation in an allogeneic mixed lymphocyte reaction ( P < 0 . 05 ) , while being unable to inhibit clustering of BDC with T cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In contrast , mAb to CR 2 or the adhesion molecules , LFA 1 , LFA 3 , or ICAM 1 , have little effect . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Dermal dendritic cells from eleven cases of mycosis fungoides ( MF ) ( six patch and five plaque stage ) , two cases of pre MF , and five specimens of normal human skin , were characterized immunohistochemically using a panel of antibodies including anti human Thy 1 , intercellular adhesion molecule 1 ( ICAM 1 ; CD 54 ) , endothelial leukocyte adhesion molecule 1 ( ELAM 1 ) , vascular cell adhesion molecule 1 ( VCAM 1 ) , CD1a , CD 2 , CD 14 , CD 18 , CD 34 , MAC 387 , KP 1 , EBM 11 , factor XIIIa , factor XIIIs , and S 100 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Adhesion molecules such as ICAM 1 and LFA 1 were also found to play an important role as cofactors in activation of lymphocytes in the antigen recognition process of EAU . ^^^ ICAM 1 and LFA 1 were also found to be involved in the infiltration process of inflammatory cells : our immunohistological examination revealed that ICAM 1 was present in the retinal pigment epithelium and epithelium of the ciliary body composing the blood ocular barrier . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
These lymphocytes expressed LFA 1 on the cell surface , and many basal cells exhibited ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
HBL 4 cells lacked CD 23 ( H 107 ) , CD11a ( LFA 1 ) , and latent membrane protein ( LMP ) but expressed CD 54 ( ICAM 1 ) at low levels , whereas HBL 5 cells showed the high level of expression of CD 54 and faint expression of LMP but lacked CD11a . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Thus , EBV infected target cells are able to interact with the principal LFA 1 ligand , intercellular adhesion molecule 1 ( ICAM 1 ; CD 54 ) , which is expressed on posttransplant peripheral blood mononuclear ( PBM ) effector cells . ^^^ Posttransplant cytotoxicity against EBV infected target cells is abolished by target and effector cell blockade with monoclonal antibodies ( MoAbs ) to LFA 1 and ICAM 1 , respectively , demonstrating the functional relevance of this additional ligand interaction . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Inhibition of the hemorrhagic response was obtained with mAb reactive with ICAM 1 as well as mAb against the leukocyte CD 18 when either was administered intravenously just before intravenous zymosan challenge . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Astrocytes after viral infection were more susceptible to alloantigen specific cytotoxic T lymphocytes and cytotoxic T lymphocyte activity was substantially reduced in the presence of mAb specific for MHC class 1 , ICAM 1 and LFA 1 but not MHC class 2 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We have assessed the role of divalent cations Mg2+ , Ca2+ , and Mn2+ in LFA 1 binding to ligand intercellular adhesion molecule 1 ( ICAM 1 ) and induction of the divalent cation dependent epitope recognized by mAb 24 . ^^^ Manganese strongly promoted both expression of the 24 epitope and T cell binding to ICAM 1 via LFA 1 , suggesting that Mn2+ is able to directly alter the conformation of LFA 1 in a manner that favors ligand binding . ^^^ Analysis of T cell binding to ICAM 1 via LFA 1 in the presence of Mg2+ or Mn2+ , confirmed that Ca2+ exerted inhibitory effects upon LFA 1 function . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
These cytokines induce synthesis and surface expression of intercellular adhesion molecule 1 ( ICAM 1 ) on the myocyte , and neutrophil adhesion is almost entirely CD 18 and ICAM 1 dependent . ^^^ Reperfusion lymph induced ICAM 1 expression in isolated myocytes , and myocyte adherence to ZAS stimulated neutrophils that was completely inhibited by anti CD 18 and anti ICAM 1 monoclonal antibodies . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies directed against LFA 1 ( CD11a / CD18 ) or its ligand ICAM 1 ( CD 54 ) did not inhibit MHC class 2 induced homotypic adhesion of various EBV transformed B cell lines , nor of a variant of the B cell line Raji expressing very low LFA 1 surface levels . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We propose that cLC are functionally resistant to low doses of UVB radiation and chemical fixation because cLC express sufficient levels of the adhesion or co stimulatory molecules [ including ICAM 1 and Mac 1 ( CD11b / CD18 ) ] required to induce T cell activation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
ICAM 1 is found on the surface of many hematopoietic and nonhematopoietic cells and can function as an adhesive ligand for the integrin , leukocyte function associated molecule 1 ( LFA 1 , CD11a / CD18 ) . ^^^ Using ELISA , cytofluorography , electron microscopy , Northern analysis , and an in vitro cell adherence assay , we demonstrate that treatment of human dermal fibroblasts with the cytokine IL 4 leads to an increase in cell surface ICAM 1 expression that is under transcriptional control as well as increased fibroblast adhesion to LFA 1 bearing T lymphocytes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To examine the role of lymphocyte function associated antigen 1 ( LFA 1 ) expression on murine B cells as it pertains to their function in T cell activation , we carried out antigen presentation assays in tissue culture wells coated with a purified , secreted form of the murine intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^ This effect was dependent on the amount of ICAM 1 used to coat the wells and was also observed in wells coated with anti LFA 1 monoclonal antibodies and was blocked by soluble anti LFA 1 antibodies . ^^^ No decrease in the antigen dose was observed if the B cells were chemically fixed or treated with ricin , or when antigen was presented by a HeLa cell line transfected with murine class 2 major histocompatibility complex ( MHC ) genes , indicating that the immobilized ICAM 1 was mediating its effect through B cell LFA 1 , and that B cell protein synthesis was required . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Plasmodium falciparum infected erythrocytes bind ICAM 1 at a site distinct from LFA 1 , Mac 1 , and human rhinovirus . ^^^ The ICAM 1 binding site is distinct from those recognized by LFA 1 , Mac 1 , and the human major type rhinoviruses . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Leukocyte cytotoxicity requires cell adhesion , which is mediated via receptors on the leukocyte surface including leukocyte function associated antigen 1 ( LFA 1 ) which binds to the ligand intercellular adhesion molecule 1 ( ICAM 1 ) on the target cell . ^^^ The distribution of ICAM 1 and LFA 1 expression in liver biopsy specimens from patients with alcoholic liver disease was examined to ascertain whether this pathway of leukocyte adhesion is involved in the tissue damage of alcoholic hepatitis . ^^^ Specimens were stained for ICAM 1 and LFA 1 by a three step immunoalkaline phosphatase method using monoclonal antibodies against ICAM 1 and LFA 1 . ^^^ LFA 1 staining on portal tract inflammatory cells and parenchymal inflammatory cells and ICAM 1 staining on liver components were examined . ^^^ ICAM 1 expression correlated with the histological degree of hepatocellular damage ( tau = 0 . 79 ; p = 0 . 0005 ) and parenchymal inflammation ( tau = 0 . 65 ; p less than 0 . 001 , and with LFA 1 expression on parenchymal leukocytes ( tau = 0 . 63 ; p = 0 . 01 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Tumor necrosis factor mediates experimental pulmonary edema by ICAM 1 and CD 18 dependent mechanisms . ( TNF alpha ) induced sequestration of neutrophils ( PMN ) in lungs and of the resultant PMN dependent pulmonary edema . ^^^ Monoclonal antibodies ( mAbs ) to the neutrophil CD 18 integrin ( beta chain of CD11 / CD18 complex ) ( mAb IB 4 ) and to its endothelial cell ligand ICAM 1 ( mAb RR1 / 1 ) were used to examine the role of PMN adhesion in the TNF alpha induced responses . ^^^ The findings indicate that TNF alpha prestimulation of lungs mediates PMN uptake and that this requires the expression of ICAM 1 and its interaction with CD 18 integrin on PMN . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Partial inhibition was observed with mAb to ICAM 1 , whereas mAb to CD 4 and LFA 1 beta were without effect . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
IL 2 induction of ICAM 1 and the maintenance of CD 18 complex expression on small lymphocytes separated by Percoll gradients were similar to that on LGL . ^^^ The expression of LFA 1 ( CD11a / CD18 ) , a receptor for ICAM 1 , and other leukocyte adhesion molecules , including Mac 1 ( CD11b / CD18 ) and p 150 , 95 ( CD11c / CD18 ) , was only maintained by IL 2 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In this study , we investigated the effects of pro inflammatory cytokines on corneal endothelial ( CE ) and stromal ( CS ) expression of intercellular adhesion molecule 1 ( ICAM 1 ) , a specialized cell surface glycoprotein that binds the leukocyte function antigen 1 ( LFA 1 ) receptor present on all leukocytes and enhances immune responses . ^^^ In parallel assays , mAb to ICAM 1 on CE cells or subunits of LFA 1 receptors on leukocytes , but not control mAb , significantly blocked leukocyte binding to unstimulated ( P less than 0 . 01 ) or rIFN gamma stimulated corneas ( P less than 0 . 001 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Mouse L cells cotransfected with both MHC class 2 ( DR2A or DR 7 ) and ICAM 1 stimulated T cells at 100 fold lower concentrations of SEA as compared to the single transfected cells . mAb reacting with the CD11a , CD 18 , or ICAM 1 molecules efficiently inhibited T cell activation with the cotransfected HLA DR2A / ICAM 1 cell but did not influence T cell activation with the HLA DR2A single transfected cell . ^^^ Naive CD4+45RA+ T cells require a costimulatory signal mediated through the LFA 1 / ICAM 1 pathway . ^^^ However , cotransfection of ICAM 1 enabled these cells to respond to low doses of SEA implicating that they are more dependent on accessory molecules than the CD4+45RO+ cells . rICAM 1 immobilized on a plastic surface , was able to strongly costimulate SEA induced T cell activation with the HLA DR2A single transfectant , suggesting that costimulatory signals mediated to the T cells through LFA 1 can be delivered physically separated from the TCR signal . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Specific acceptance of cardiac allograft after treatment with antibodies to ICAM 1 and LFA 1 . ^^^ An indefinite survival of cardiac allografts between fully incompatible mice strains was observed when monoclonal antibodies ( MAbs ) to intercellular adhesion molecule 1 ( ICAM 1 ) and leukocyte function associated antigen 1 ( LFA 1 ) were simultaneously administered after the transplantation for 6 days . ^^^ Because MAbs to ICAM 1 or LFA 1 alone were insufficient for prolonged tolerance , the two MAbs probably acted synergistically to induce specific unresponsiveness . ^^^ Thus , ICAM 1 LFA 1 adhesion participates in the induction of allograft rejection and MAbs may be useful as therapeutic agents . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Dendritic cells displayed high forward and low intermediate side angle scatter , and expressed high levels of major histocompatibility complex ( MHC ) class 1 and class 2 molecules , the heat stable antigen ( HSA ) , the adhesion molecules Pgp 1 ( CD 44 ) , LFA 1 , ICAM 1 and low levels of Mac 1 and the leukocyte common antigen CD 45 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Presently , two cellular ligands for LFA 1 have been identified , termed ICAM 1 and ICAM 2 . ^^^ These studies provide evidence that ICAM 1 and ICAM 2 are not important target cell ligands for NK effector cell LFA 1 and that other target cell ICAM may exist . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Expression of the CD 54 ( ICAM 1 ) and CD11a ( LFA 1 ) adhesion molecules in oral mucosal inflammation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Fresh frozen sections from 12 monoclonal CNS NHLs ( 11 primary , one secondary ) were stained with monoclonal antibodies to LFA 1 alpha chain ( CD11a ) , beta chain ( CD 18 ) and , ICAM 1 ( CD 54 ) . ^^^ Normal SCID mouse brains showed no staining with CD11a , CD 18 , CD 54 or mouse ICAM 1 antibodies . ^^^ Expression of LFA 1 / ICAM 1 in CNS lymphomas : possible mechanism for lymphoma homing into the brain . ^^^ We examined a possible role for the adhesion molecules LFA 1 and ICAM 1 in localizing central nervous system non Hodgkin ' s lymphomas ( CNS NHLs ) to the brain . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Lymphocyte endothelial adherence is , in part , caused by the leukocyte integrin LFA 1 binding to ICAM 1 , its ligand on endothelial cells . ^^^ These studies predict potential sites for interaction of the integrin receptor , LFA 1 , with its ligand , ICAM 1 . ^^^ Thus lymphocyte endothelial interaction , and resulting inflammation , may be partially mediated by the association of ICAM 1 with LFA 1 at the specific molecular locations identified in this study . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Regulation of locomotion and cell cell contact area by the LFA 1 and ICAM 1 adhesion receptors . ^^^ We demonstrate complementary differences in the behavior of B lymphoblastoid cells adhering to LFA 1 or its counter receptor ICAM 1 . ^^^ The interaction of B lymphoblastoid cells with glass supported planar bilayers bearing LFA 1 or ICAM 1 was observed by time lapse video microscopy , and the distribution of adhesion receptors on cells interacting with the planar bilayers was studied by immunofluorescence microscopy . ^^^ Cellular LFA 1 remained uniformly distributed on the cell surface during interaction with bilayers bearing purified ICAM 1 as determined by immunofluorescence . ^^^ In contrast , ICAM 1 was concentrated in the stalk like structure through which the unstimulated B lymphoblasts adhered to LFA 1 in planar bilayers , but ICAM 1 immunofluorescence became more uniformly distributed over the cell surface within minutes of phorbol ester addition . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The role of the cytokines IL 1 alpha , IL 1 beta , and IL 6 and the cell adhesion molecules ICAM 1 , LFA 1 ( alpha and beta ) , and Mac 1 as accessory molecules for stimulation of T cells by the superantigen staphylococcal enterotoxin B ( SEB ) was examined . ^^^ The cell adhesion molecules ICAM 1 and LFA 1 but not Mac 1 also functioned as accessory molecules for SEB induced cluster formation and lymphocyte blastogenesis . ^^^ Thus , not only must this superantigen bind to Class 2 MHC on accessory cells as is well known , but also SEB requires at least certain cytokines ( IL 1 alpha and IL 1 beta ) produced by accessory cells and cell adhesion molecules ( ICAM 1 and LFA 1 ) for activation of T lymphocytes . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule 1 ( ICAM 1 , CD 54 ) is a cell surface ligand for LFA 1 ( CD11a / 18 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
For this purpose , we tested the HEV binding ability and the expression of the lymphocyte homing receptor ( LHR ) for peripheral lymph nodes as well as Pgp 1 / CD44 , LFA 1 and ICAM 1 adhesion molecules by the highly leukemic cell line NQ 22 in comparison with a series of non leukemic murine T lymphoma cell lines . ^^^ In addition , our findings seem to rule out that gross quantitative modifications in LFA 1 or ICAM 1 antigen expression are associated with differential dissemination abilities of transformed lymphoid cells . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Ultrastructural identification and distribution of the adhesion molecules ICAM 1 and LFA 1 in the vascular and extravascular compartments of the human palatine tonsil . ^^^ Immunohistological analysis of sections prepared from human palatine tonsils revealed marked differences in the distribution of the adhesion molecule , leucocyte function antigen 1 ( LFA 1 ) and its counter receptor , intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^ We propose that LFA 1 / ICAM 1 binding participates in mediating the transendothelial migration of lymphocytes across the high endothelial venules of palatine tonsil . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
This A / R induced hyperadherence was significantly diminished by monoclonal antibodies ( MAb ) directed at CD11a , CD11b , CD 18 or ICAM 1 , but not by MAb directed at ELAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Co engagement of CD 3 with LFA 1 or ICAM 1 adhesion molecules enhances the frequency of activation of single murine CD4+ and CD8+ T cells and induces synthesis of IL 3 and IFN gamma but not IL 4 or IL 6 . ^^^ Interaction between the cell adhesion molecules lymphocyte function associated antigen 1 ( LFA 1 ) and intercellular adhesion molecule 1 ( ICAM 1 ) augments T cell activation by increasing the avidity of T cell / antigen presenting cell ( APC ) binding . ^^^ To examine whether LFA 1 and ICAM 1 can also contribute to T cell activation in the absence of APCs , single murine CD4+ and CD8+ T cells were cultured with IL 2 and immobilized antibodies to CD 3 , CD 4 or CD 8 , and LFA 1 or ICAM 1 . ^^^ It is concluded that co stimulation of T cells via LFA 1 or ICAM 1 can enhance T cell receptor dependent activation in the absence of accessory cells and that this mode of stimulation leads to the expression of a restricted range of cytokine genes . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The role of neutrophils ( PMNs ) in the pathogenesis of microvascular injury was investigated by inhibition of PMN adherence to the microvascular endothelium using monoclonal antibodies directed to the leukocyte CD 18 adhesion complex or its endothelial ligand , intercellular adhesion molecule 1 ( ICAM 1 , CD 54 ) . ^^^ There were five experimental groups ; controls given saline alone , n = 12 ; animals given monoclonal antibody to the PMN CD 18 complex , R 15 . 7 prior to burn injury ( pre R15 . 7 , n = 5 ) ; animals given R 15 . 7 30 min after burn injury ( post R 15 . 7 , n = 6 ) ; animals given the anti ICAM 1 antibody , R 6 . 5 prior to burn ( pre R 6 . 5 , n = 6 ) ; and animals given the R 6 . 5 30 min postburn injury ( post R 6 . 5 , n = 6 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The expression of HLA class 1 , HLA DR , HLA DP , HLA DQ , intercellular adhesion molecule 1 ( ICAM 1 / CD54 ) , lymphocyte function associated ( LFA ) molecules LFA 1 ( CD11a / CD18 ) , LFA 2 ( CD 2 ) , and LFA 3 ( CD 58 ) was investigated by FACS analysis before and after induction for various times with human rIFN gamma , TNF alpha , or both . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Mapping the sites of interaction of ICAM 1 with LFA 1 , the receptor for ICAM 1 on lymphocytes , may lead to the design of novel inhibitors of inflammation or metastasis . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
However , blocking of ICAM 1 with antibodies could only partially inhibit the increased LFA 1 dependent lysis . ^^^ This supports recent evidence for the existence of an additional ligand for LFA 1 , other than ICAM 1 and ICAM 2 . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In order to study how inflammatory cells including autoimmune lymphocytes interact with each other to develop collagen induced arthritis ( CIA ) , we injected monoclonal antibodies against mouse LFA 1 and ICAM 1 into DBA / 1 mice immunized with type 2 collagen ( CII ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
This notion is supported by the temperature and divalent cation dependence which is characteristic of LFA 1 mediated adhesion . mAb against ICAM 1 ( CD 54 ) did not block LFA 1 alpha induced adhesion . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Lymphocyte adhesion to target cells is mediated , in part , by the interaction of lymphocyte function associated Ag 1 ( LFA 1 ) with intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^ The adhesion augmented by increased [ cAMP ] 1 was due to LFA 1 / ICAM 1 interactions between cells because it was blocked by either anti LFA 1 or anti ICAM 1 mAb . ^^^ Elevated [ cAMP ] 1 induced cell surface patching of LFA 1 , but not ICAM 1 , and this redistribution preceded intercellular adhesion . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Ag independent adhesion between lymphocytes and target cells is mediated in part by the interaction between lymphocyte function associated Ag 1 ( LFA 1 ) and its coreceptor intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^ Within minutes , PMA treatment of JY cells , which express both LFA 1 and ICAM 1 , induced capping of LFA 1 and augmentation of intercellular adhesion lasting for several hours . ^^^ Intercellular adhesion , due to LFA 1 / ICAM 1 interactions , could also be induced by LPS treatment of JY cells . ^^^ These results suggest a model of sequential induction , inhibition , and reinduction of LFA 1 / ICAM 1 mediated lymphocyte adhesion that is regulated by temporally ordered actions and interactions of protein kinases C and A . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
It has previously been shown that transfection of the LMP 1 and EBNA 2 genes into Burkitt ' s lymphoma cell lines results in the up regulation of CD 23 , CD 21 , ICAM 1 and LFA 1 cell surface proteins . ^^^ We show that transient expression of the LMP 1 gene , from heterologous promoters , is sufficient to induce cellular enlargement and up regulation of surface expression of the activation markers CD 23 , CD 21 , ICAM 1 and LFA 1 in primary B cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Regulation of the expression of leukocyte function associated antigen 1 ( LFA 1 ) and intercellular adhesion molecule 1 ( ICAM 1 ) on a human eosinophilic leukemia cell line EoL 3 . ^^^ The effects of several cytokines and phorbol myristate acetate ( PMA ) on LFA 1 and ICAM 1 expression on a human eosinophilic leukemia cell line , EoL 3 , were investigated and compared with those of a human monocytic leukemia cell line , U 937 . ^^^ EoL 3 cells expressed large amounts of LFA 1 and small amounts of ICAM 1 , and their expression was regulated similarly in EoL 3 cells and U 937 cells . ^^^ Interferon gamma ( IFN gamma ) enhanced ICAM 1 expression but not LFA 1 expression , and PMA augmented both LFA 1 and ICAM 1 expression . ^^^ These results collectively suggest that expression of LFA 1 and ICAM 1 is regulated differently and that IFN gamma and PMA regulate the expression through different mechanisms . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Expression of the beta 2 integrins ( LFA 1 , CD11a / CD18 ; MAC 1 , CD11b / CD18 ; p 150 , 95 , CD11c / CD18 ) and ICAM 1 ( CD 54 ) by monocytes and granulocytes was studied using flow cytometry . ^^^ OM 85 enhanced the expression of MAC 1 and ICAM 1 on monocytes and granulocytes in a dose dependent manner . ^^^ While MAC 1 and p 150 , 95 expression reached peak values between 1 and 6 h , levels of ICAM 1 rose constantly for 10 h . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The expression of three alpha chains designed as CD11a , CD11b , CD11c , a common beta chain CD 18 , and of a ligand for several integrins CD 54 ( ICAM 1 ) was studied on alveolar macrophages of patients with active and inactive sarcoidosis and in control subjects . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Regulation of the avidity of LFA 1 ( CD11a / CD18 , alpha L beta 2 ) for its ligand ICAM 1 ( CD 54 ) was studied in human B cells by evaluating the effects of a phorbol ester , anti IgM antibodies , staurosporine , and okadaic acid . ^^^ In this assay , the protein kinase C activating phorbol ester PDB and anti IgM antibodies , as well as the protein kinase inhibitor , staurosporine , were able to induce LFA 1 dependent binding to ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies targeting murine LFA 1 induce LFA 1 / ICAM 1 independent homotypic lymphocyte aggregation . ^^^ The LFA 1 alpha induced homotypic aggregation is inhibited with LFA 1 beta antibodies , but not with antibodies targeting ICAM 1 , VCAM 1 , VLA 4 , or CD 2 . 3A9 cells do not express the LFA 1 ligand ICAM 1 , whereas EL 4 cells express moderate amounts of ICAM 1 . ^^^ Thus , targeting LFA 1 alpha with mAb results in homotypic aggregation of T cell lines which is independent of ICAM 1 / LFA 1 interactions , but may involve other LFA 1 ligands such as ICAM 2 or ICAM 3 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Ligands to three of these molecules have been identified ; ICAM 1 , LFA 3 , and hyaluronic acid binding to LFA 1 , LFA 2 , and CD 44 , respectively . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Strong induction of ICAM 1 in human T cells transformed by human T cell leukemia virus type 1 and depression of ICAM 1 or LFA 1 in adult T cell leukemia derived cell lines . ^^^ Sixteen human T cell lines were studied for the expression of a cell adhesion molecule ICAM 1 and its counter receptor LFA 1 . ^^^ It was also found that ICAM 1 and LFA 1 were involved in syncytium formation induced in the co culture of HTLV 1 positive and HTLV 1 negative human T cell lines . ^^^ Implications of constitutive expression of ICAM 1 for certain clinical manifestations of ATL and of depression of either ICAM 1 or LFA 1 during progression of ATL are discussed . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
ICAM 1 expression allows adherence of activated neutrophils to myocytes that is blocked by anti CD 18 mAb , R15 . 7 , or anti ICAM 1 mAb , CL18 / 6 . ^^^ Fluorescence and neutrophil / myocyte adherence followed the same time course , and both were blocked by monoclonal antibodies to CD 18 , CD11b , and ICAM 1 , but mAb R7 . 1 , recognizing a functional epitope on CD11a , was not inhibitory . ^^^ Thus , adherence dependent on Mac 1 ( CD11b / CD18 ) and ICAM 1 ( CD 54 ) activates the neutrophil respiratory burst resulting in a highly compartmented iron dependent myocyte oxidative injury . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule 1 ( ICAM 1 ) and lymphocyte function asociated antigen 1 ( LFA 1 ) are cell surface adhesion receptors that bind to one another and promote a variety of effector / target cell interactions in tissues affected by inflammatory or immune processes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The expression of intercellular adhesion molecule 1 ( ICAM 1 ) , a ligand for LFA 1 which is expressed on all leucocytes , was not significantly different from normal , whereas HLA DR was expressed more intensely on sarcoid monocytes ( P less than 0 . 01 ) and blood lymphocytes ( P less than 0 . 005 ) than control cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Expression of leukocyte adhesion molecules ( ICAM 1 / LFA 1 ) related to clinical behaviour in B cell lymphomas . ^^^ We investigated the expression of adhesion molecules of lymphocyte function associated antigen 1 alpha ( LFA 1 alpha ) and its ligand intercellular adhesion molecule 1 ( ICAM 1 ) on 74 well characterized B cell lymphomas . ^^^ The LFA 1 was expressed on B cell lymphomas ( 21 / 74 ; 28 per cent ) , but to a lesser degree than ICAM 1 which was highly expressed ( 48 / 74 cases ; 64 per cent ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Force contribution of the LFA 1 / ICAM 1 complex to T cell adhesion . ^^^ To determine the forces involved in adhesion dependent on lymphocyte function associated antigen 1 ( LFA 1 ) and intercellular adhesion molecule 1 ( ICAM 1 ) , we have measured the junctional avidity between single cell pairs consisting of a cloned T cell that expresses LFA 1 and a fibroblast cell that expresses MHC class 2 molecules and ICAM 1 after transfection . ^^^ T cell adhesion to three related fibroblast cell lines was compared : the parent line that does not express ICAM 1 or other LFA 1 counter receptors , and two transfectants that have high and moderate levels of surface ICAM 1 expression . ^^^ The dependence of adhesion strength on the LFA 1 / ICAM 1 complex was confirmed by the use of blocking antibodies , which showed the contribution from the interaction of CD4 / MHC class 2 to be negligible . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
This study tested the hypothesis that preventing neutrophil adhesion during reperfusion , by blocking either the neutrophil membrane CD 18 integrin complex or its endothelial and myocyte ligand , intercellular adhesion molecule 1 ( ICAM 1 ) , would reduce myocardial inflammation and edema and improve reflow and ventricular function after heart preservation and transplantation . ^^^ Forty five minutes before reperfusion , recipients of preserved hearts received intravenous infusions of either saline ( vehicle , n = 13 ) , anti CD 18 monoclonal antibody ( Mab ) R15 . 7 ( 2 mg / kg ) ( anti CD 18 , n = 10 ) , or anti ICAM 1 Mab R1 . 1 ( 2 mg / kg ) ( anti ICAM , n = 10 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In addition , the activation antigens ( HLA DR ) and two adhesion molecules , including the alpha chain of the leukocyte function associated antigen 1 ( LFA 1 alpha : CD11a ) and its ligand ( intercellular adhesion molecule 1 : ICAM 1 : CD 54 ) expressed on T cells , were compared . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Activation of resting human CD4+ T cells mediated by mAb ligation of the TCR / CD3 complex requires costimulatory signals to result in proliferation ; these can be provided by intercellular cell adhesion molecule 1 ( ICAM 1 , CD 54 ) a natural ligand of leukocyte function associated Ag 1 ( LFA 1 , CD11a / CD18 ) . ^^^ We analyzed early signaling events involved in T cell activation to determine the contribution by the LFA 1 / ICAM 1 interaction . ^^^ Our investigations show no response to LFA 1 / ICAM 1 alone , but that costimulation by LFA 1 / CAM 1 interaction induces prolonged inositol phospholipid hydrolysis ( up to 4 h ) , resulting in generation of both inositol ( 1 , 4 , 5 ) phosphate 3 and inositol ( 1 , 3 , 4 , 5 ) phosphate 4 and their derivatives . ^^^ A sustained increase in intracellular levels of free Ca2+ level is also observed after LFA 1 / ICAM 1 costimulation , which is at least partly dependent on extracellular sources of Ca2+ . ^^^ Kinetic studies indicate that costimulation requires a minimal period of 4 h of LFA 1 / ICAM 1 interaction to provide maximal costimulation for OKT 3 mediated T cell proliferation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
LFA 1 and ICAM 1 molecule expression in jejunal mucosa from children with autoimmune enteropathy . ^^^ Jejunal biopsies were stained by IFL with monoclonal antibodies to LFA 1 ( TS1 / 22 and CD11a / 25 . 3 . 1 ) and ICAM 1 ( RR1 / 1 and 84H10 ) molecules . ^^^ LFA 1 and ICAM 1 positive cells were observed in the lamina propria in all cases and the counts were increased in autoimmune enteropathy compared with controls . ^^^ In addition , in 4 of 7 cases of autoimmune enteropathy crypt enterocytes were positives for ICAM 1 when stained with RR1 / 1 and 3 of the 4 were also positive for LFA 1 when stained with both LFA 1 reagents . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule 1 ( ICAM 1 , CD 54 ) is a cell adhesion molecule that interacts with the leukocyte beta 2 integrins , LFA 1 and Mac 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
One of the systems implicated in these interactions involves an integrin , LFA 1 ( Lymphocyte Function Antigen 1 ) , expressed by all leukocytes at their cell surface , and a molecule belonging to the immunoglobulin superfamily , ICAM 1 ( Intercellular Adhesion Molecule 1 ) . ^^^ The avidity of LFA 1 for ICAM 1 is transient . ^^^ It is modulated both by regulation of ICAM 1 expression and by activation of LFA 1 molecules constitutively expressed on leukocyte membranes . ^^^ In addition to being a ligand for LFA 1 , ICAM 1 is sometimes used as a cell receptor by pathogens such as Plasmodium falciparum , the causative organism of malaria . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In cold target competition assays , CLL cells did not compete with labelled K 562 or Daudi targets in the NK and LAK assays , suggesting that the malignant cells are not recognised by the effector cells , and this may be related to low level of expression of the adhesion receptors , LFA 1 and ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In this study we investigated the differential effect of the co stimulatory receptor ligand molecules CD2 / LFA 3 , LFA 1 / ICAM 1 , and CD28 / B7 on microbial superantigen mediated activation of CD4+ T cells . ^^^ The K 562 plus SEB induced response can be inhibited by combinations of mAbs to CD 2 and LFA 1 , and to LFA 3 , ICAM 1 , and B 7 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
As a model , the contribution of LFA 1 ( CD11a / CD18 ) and ICAM 1 ( CD 54 ) in adhesion of PBMC to EC was studied . ^^^ It was found that adhesion of lymphocytes and monocytes is regulated differently by phorbol ester and that the relative contribution of LFA 1 and ICAM 1 differs for both cell types . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In order to examine the role of target cell intercellular adhesion molecule 1 ( ICAM 1 ; CD 54 ) , a ligand of lymphocyte function associated antigen ( LFA 1 ) ( CD11a / CD18 ) , we exposed the human leukemia cell line , HL 60 , to a variety of agents implicated in modulating ICAM 1 expression and / or sensitivity to lymphocyte cytolysis . ^^^ Although our results suggest a role for leukocyte CD11 / CD18 adhesion molecules in LAK cytolysis , the poor correlation between ICAM 1 expression and sensitivity to LAK lysis suggest that interactions other than LFA 1 / ICAM 1 conjugation may be more central to the processes involved . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule type 1 ( ICAM 1 ) , lymphocyte function associated antigen type 1 ( LFA 1 ) , LFA 3 , CD 2 , and CD 44 expression were determined using indirect immunofluorescence techniques . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The staining was , however , restricted to the luminal membrane of the epithelial cells , raising doubts about the role of ICAM 1 as a ligand for LFA 1 on mucosal leucocytes in rectal GvHD . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Since we were unable to reduce significantly the adhesion of cultured normal LFA 1+ T cells to 24 h with TNF alpha stimulated endothelium with antibodies that block LFA 1 / ICAM 1 or VLA 4 / VCAM 1 interactions , and lectin adhesion molecule 1 and endothelial leukocyte adhesion molecule 1 appeared not to be implicated , other as yet undefined cell surface structures are likely to participate in T cell / EC interactions . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Stimulation of cells with antibodies to the monocyte surface Ag MAC 1 , LFA 1 , and ICAM 1 did not result in M CSF secretion . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To be able to further study interactions between the adhesive cell surface molecule LFA 1 and its ligand ICAM 1 , we have produced an ICAM 1 fusion protein in which the extracellular part of ICAM 1 has been fused to a part of an IgG heavy chain . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The cells strongly expressed CD 25 when stimulated with OK 432 and exhibited a high level of cytotoxic activity in part explained by the increased expression of ICAM 1 and LFA 1 , and the release of TNF beta . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The intercellular adhesion molecule 1 ( ICAM 1 ) is a cell membrane glycoprotein displaying a pivvtal role in cell cell interactions in the immune system , and is a ligand for LFA 1 , which is expressed on leukocytes . ^^^ The ICAM 1 expression on a subset of KC in normal skin might account for the trafficking to and from normal epidermis of LFA 1 positive cells , including migrating Langerhans cells and occasional leukocytes . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
LFA 1 ( leukocyte function associated antigen 1 ) molecules were seen as granules on the surfaces of all infiltrates , most of which also expressed ICAM 1 . ^^^ These findings may help to explain the differing histological features of MF and LR : ICAM 1 molecules present on the intercellular junctions of MF epidermis lead the LFA 1 bearing cells to migrate into the interspaces , thus producing epidermotropism . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
On AC expressing high levels of CD 54 ( intercellular adhesion molecule 1 , ICAM 1 ) and CD 58 ( lymphocyte function associated antigen 3 , LFA 3 ) , mAb to CD 2 were relatively ineffective in inhibiting the response to the toxins and antibodies to the lymphocyte function associated antigen 1 ( LFA 1 ) did not inhibit at all . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
SCF / KL treatment did not alter expression of gpIb , gpIIb / IIIa , LFA 1 , ICAM 1 , or GMP 140 in CMK cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Expression of ICAM 1 ( CD 54 ) , a ligand for LFA 1 , was also found to be enhanced with a concomitant increase of ICAM 1 mRNA levels . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
No correlation with the expression of other AM ( intercellular adhesion molecule 1 ( ICAM 1 ) , leucocyte function antigen 1 ( LFA 1 ) . ^^^ In the case of B prolymphocytic leukaemia ( B PLL ) which did not express ICAM 1 or LFA 1 but was NCAM+ , a complete resistance to LAK activity and lack of conjugate formation was observed . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Pop . 3 cells also express high levels of LFA 1 , ICAM 1 , and ICAM 2 , and a subset of them are VLA 4 alpha positive . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Leukocyte beta 1 ( CD 29 ) and beta 2 ( CD 18 ) integrins and their respective ligands , ICAM 1 and VCAM 1 , on neural cells appear to be the dominant ligands mediating MNL : neural cell adhesive interactions . mAb to CD 18 block 32 to 57 % of the MNL binding to neural cells ; similar inhibition is seen with mAb to ICAM 1 . mAb to CD 29 block 16 to 17 % of the MNL binding to the neural cells suggesting that leukocyte beta 1 integrins and neural VCAM 1 may be a second route for MNL : neural cell interactions . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Within the brain parenchyma , ICAM 1 and LFA 3 were found on astrocytes and tumor cells ; on the contrary , LFA 1 was expressed on microglial cells similar to CR 3 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
These included the alpha 4 beta 1 ( VLA 4 ) , alpha 5 beta 1 ( VLA 5 ) , and alpha L beta 2 ( LFA 1 ) integrins , as well as ICAM 1 , LFA 3 , H CAM , and LAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Blocking LFA 1 , LFA 3 , and ICAM 1 with monoclonal antibodies inhibited the adherence of T cells to thyroid follicular cells in assays of cell clustering ; antibodies against ICAM 2 had no effect . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule 1 ( ICAM 1 ) is the ligand for lymphocyte function associated antigen 1 ( LFA 1 ) , mediating the adhesion of lymphocytes to vascular endothelium . ^^^ Using an indirect immunofluorescence method , we examined the patterns of ICAM 1 and LFA 1 staining in bullous pemphigoid ( BP ) lesions and compared them to pemphigus vulgaris ( PV ) cases . ^^^ It is known that ICAM 1 is expressed on keratinocytes at the site of lymphoid infiltration in cutaneous dermatoses and that LFA 1 positive T cells can bind to interferon gamma induced ICAM 1 positive keratinocytes . ^^^ Our results suggest that cellular immunity involving ICAM 1 and LFA 1 may play a part in the pathogenesis of bullous pemphigoid . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
They are also positive for the expression of CD45RA molecules and some express the ICAM 1 and the LFA 1 molecules . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Pretreating gamma delta cells with monoclonal antibodies directed at either LFA 1 or VLA 4 molecules or pretreating EC with monoclonal antibodies directed against either ICAM 1 or VCAM 1 molecules significantly inhibited gamma delta cell mediated enhancement in endothelial permeability . ^^^ This indicated that VLA 4 / VCAM 1 and LFA 1 / ICAM 1 adhesion pathways participate in gamma delta cell EC interaction . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Homogeneous marrow stromal cell monolayers capable of supporting proliferation of cocultivated CD34hi cells expressed VCAM 1 and fibronectin ( ligands for VLA 4 and VLA 5 ) as well as ICAM 1 ( ligand for LFA 1 and Mac 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
ICAM 1 appeared to mediate adhesion of T lymphocytes by binding to the beta 2 integrin CD11a / CD18 ( LFA 1 ) expressed by the lymphoblasts . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Other molecules involved in cell / cell adhesion are LFA 1 ( CD11a / CD18 ) , Mac 1 ( CD11b / CD18 ) and their ligand ICAM 1 ( CD 54 ) , p 150 , 95 ( CD11c / CD18 ) , LFA 3 ( CD 58 ) , CD 44 , and LECAM 1 . ^^^ Thymic B cell lymphomas consistently lacked VLA 1 , 2 , 3 , 5 , 6 , and CD11b , expressed ICAM 1 in 21 of 23 cases but were heterogenous for VLA 4 , LFA 1 , CD11c , LFA 3 , CD 44 , and LECAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We used monoclonal antibodies ( mAb ) to adhesion molecules on endothelial cells ( i . e . , endothelial leukocyte adhesion molecule 1 ( ELAM 1 ) and intercellular adhesion molecule 1 ( ICAM 1 ) ) or on PMN ( CD 18 ) to assess the role of PMN adhesion to the activated endothelium on LTB 4 potentiation . ^^^ Furthermore , combination of anti ELAM 1 mAb ( H18 / 7 ) and anti ICAM 1 mAb ( RR1 / 1 ) or of anti ELAM 1 mAb ( H18 / 7 ) and anti CD 18 mAb ( IB 4 ) had an additive effect in inhibiting both PMN adhesion as well as LTB 4 production . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The endothelial staining of these CAMs was related to the number of vessels within each biopsy , delineated by a monoclonal antibody against Ulex europaeus 1 lectin bound to endothelial cells , and to the number of tissue leukocytes staining for one of the ligands of ICAM 1 , the beta 2 integrin , lymphocyte function associated antigen ( LFA 1 ) . ^^^ LFA 1 expression significantly correlated with the number of ICAM 1 positive vessels ( P less than 0 . 03 ) . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
LFA 1 alpha ( CD11a ) and LFA 1 beta ( CD 18 ) and their ligand ICAM 1 ( CD 54 ) , H CAM ( lymphocyte homing receptor ; CD 44 ) and N CAM ( CD 56 ) . ^^^ LFA 1 alpha ( CD11a ) and LFA 1 beta ( CD 18 ) and their ligand ICAM 1 ( CD 54 ) , H CAM ( lymphocyte homing receptor ; CD 44 ) and N CAM ( CD 56 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The accessory molecules we have identified as critical are LFA 1 and CD2 / LFA 2 on LAK T cells interacting with their respective ligands ICAM 1 and LFA 3 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
MoAbs specific for ICAM 1 or its ligand LFA 1 inhibited adhesion of PBL to either IFN gamma or TNF alpha pretreated renal cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Appropriate experimental conditions were devised to demonstrate that CD 58 ( LFA 3 ) , CD 54 ( ICAM 1 ) and CD11a / CD18 ( LFA 1 ) adhesion molecules are the source of signals that regulate nonspecific major histocompatibility complex unrestricted and CD3 / T cell receptor ( TcR ) triggered cytotoxicity . ^^^ We conclude that mAb against LFA 3 , ICAM 1 and LFA 1 molecules deliver regulatory signals for LAK cells and cytotoxic T lymphocytes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In the study reported here , the distribution of the adhesion receptors LFA 1 ( CD11a / CD18 ) , ICAM 1 ( CD 54 ) , CD 2 and LFA 3 ( CD 58 ) in recurrent oral ulcers ( ROU ) were studied . ^^^ The findings indicate that LFA 1 / ICAM 1 and CD2 / LFA 3 interactions may play roles in cell to cell adhesion events in ROU . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The expression of the immunoglobulin related molecules CD 2 , CD 54 ( ICAM 1 ) and CD 58 ( LFA 3 ) was higher on RA SFL when compared to RA PBL or CO PBL , and similar results were obtained with the beta 2 integrin subunits CD11a and CD 18 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The later prolonged PMN adhesion was ICAM 1 dependent since this response was inhibited by mAb RR1 / 1 and to the same degree by the anti CD 18 mAb IB 4 . ^^^ Thrombin induced PMN adhesion is stabilized by a protein synthesis independent upregulation of the constitutive ICAM 1 activity which enables the interaction of ICAM 1 with the CD 18 beta 2 integrin on PMN . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
These new data showing that ICAM 1 , ICAM 2 and LFA 3 are , like LFA 1 , expressed on HC emphasize the ability of HC to act as APC . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
LFA 1 dependent adhesion was mediated by ligands on HEC other than the intercellular adhesion molecule 1 ( ICAM 1 ) and the VLA 4 related pathway was mediated by ligands other than the CS 1 domain of fibronectin . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Neither MAC 1 ( CD11b / CD18 ) nor ICAM 1 was observed on DCs . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The delayed acquisition of APC function can not be accounted for solely by a deficiency in major histocompatibility complex MHC class 2 , ICAM 1 , or LFA 1 as neonatal B cells express adult levels of these molecules by 7 14 days after birth . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In contrast to chronic PSS , LFA 1 alpha , LFA 1 beta and ICAM 1 expression on mononuclear infiltrating cells was seen more frequently in acute scleroderma . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Using such models , we and others have obtained evidence that both lymphocyte function associated antigen 1 ( LFA 1 ) / ICAM 1 and very late activation antigen 4 ( VLA 4 ) / VCAM 1 interactions mediate lymphocyte adhesion to endothelial cells , but that LFA 1 / ICAM 1 interactions play a greater role in transendothelial migration . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Priming of CD4+ T cells with ICAM 1 induced higher expression of both CD 18 and CD 28 than that with either B 7 or ICAM 2 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
This receptor based immunoadhesin is an effective and specific inhibitor of P . falciparum infected erythrocyte adhesion to ICAM 1 bearing surfaces , but does not inhibit leukocyte function antigen 1 ( LFA 1 ) interaction with ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
ICAM 3 is potentially the most important ligand for LFA 1 in the initiation of the immune response because the expression of ICAM 1 on resting leukocytes is low . ^^^ ICAM 3 is closely related to ICAM 1 , consists of five immunoglobulin domains , and binds LFA 1 through its two N terminal domains . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In common with ICAM 1 and ICAM 2 , ICAM R is a ligand for the beta 2 integrin CD11a / LFA 1 ( CD 18 ) . . ^^^ It has been found that cell cell adhesion which is dependent on expression of the leukocyte function associated antigen LFA 1 is not always blocked completely by antibodies raised against ICAM 1 and ICAM 2 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
These include LFA 1 alpha and beta chains ( CD11a , CD 18 ) , and ICAM 1 ( CD 54 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
As normal and inflamed intestinal epithelia do not express ICAM 1 , it seems unlikely that the LFA 1 / ICAM 1 interaction is of importance to IEL activation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
This correlated with decreased expression of the surface molecules ICAM 1 and CD 18 as determined by fluorescence activated cell sorter analysis . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
DC expressed LFA 1 , ICAM 1 , LFA 3 , and the Hermes antigen , but no other integrin or immunoglobulin supergene family adhesion molecules were detected using a sensitive immunoperoxidase staining technique . ^^^ The simultaneous addition of monoclonal antibodies to MLRs and preincubation washing experiments established that DC have at least 3 independent adhesion ligand interactions ( LFA 1 ICAM 1 , ICAM 1 LFA 1 , and LFA 3 CD 2 ) with T cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The infiltrating cells expressed LFA 1 and CD 2 , the correlating ligands for ICAM 1 and LFA 3 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Direct cellular interactions , involving adhesion structures like lymphocyte function associated antigen 1 ( LFA 1 ) and intercellular adhesion molecule 1 ( ICAM 1 ) , play a critical role in the initial stages of T cell dependent B cell activation . ^^^ Fluorescence activated cell sorter ( FACS ) analysis showed that the expression of both LFA 1 and ICAM 1 increased after stimulation with IL 4 as well as with IFN gamma . ^^^ However , in contrast to IL 4 , IFN gamma did not enhance cellular aggregation , suggesting that qualitative rather than quantitative changes in LFA 1 / ICAM 1 promote aggregation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The adhesion / activation molecules ICAM 1 , LFA 1 , and CD 44 supported both cluster formation and proliferation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Moreover double stainings revealed that most of the S 100 beta + dendritic cells and CD68+ macrophages were intercellular adhesion molecule 1 ( ICAM 1 , a ligand for LFA 1 ) positive . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Expression of MHC class 2 molecule HLA D and of adhesion molecules LFA 1 , LFA 3 , and ICAM 1 was clearly reduced by DB , giving an explanation for the observed inhibition of cluster formation between T cells and APC / AC . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion in lymphocytes is mediated in part by the interaction of the integrin lymphocyte function associated antigen 1 ( LFA 1 ) with intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^ The B lymphoblastoid line JY expresses both LFA 1 and ICAM 1 , and intercellular adhesion is enhanced by treatment with the phorbol ester phorbol 12 myristate 13 acetate ( PMA ) , which also induced capping of LFA 1 , ICAM 1 , and human leukocyte antigen . ^^^ Taken together , these data suggest that intercellular adhesion is the result of PKC mediated membrane redistribution of LFA 1 and ICAM 1 , which is in turn associated with modification of the actin based cytoskeleton . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
VLA 2 , VLA 5 , VLA 6 , LFA 1 , Mac 1 , p150 / 95 , beta 2 chain , HCAM , ICAM 1 , NCAM , and VCAM 1 were more expressed on ' normal ' as compared with ' leukemic ' bone marrow stromal cells , although this reached significance only for beta 2 chain and NCAM . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The expression of VLA 1 , 3 , 4 , 5 , 6 , beta 1 chain , LFA 1 , beta 2 chain , ICAM 1 and NCAM appeared to be higher in bone marrow as compared to peripheral blood leukaemic cells , although this only reached significance for beta 1 chain ( p less than 0 . 01 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
PMA induced intercellular adhesion molecule 1 ( ICAM 1 ) expression on KC ; furthermore , mAb against ICAM 1 or against its ligand lymphocyte function associated Ag ( LFA 1 ) ( CD11a / CD18 ) significantly , but incompletely , reduced the stimulatory capacity of PMA treated KC , indicating that ICAM 1 / LFA 1 interaction contributed to PBMC proliferation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Adhesion molecules CD11a , CD 18 , and ICAM 1 on human epidermal Langerhans cells serve a functional role in the activation of alloreactive T cells . ^^^ Human epidermal cells ( EC ) , Ficoll enriched for LC ( greater than 10 % ) , were incubated with monoclonal antibodies ( MoAb ) specific for the adhesion molecules CD11a ( LFA 1 alpha ) , CD 18 ( LFA 1 beta ) , or ICAM 1 ; staining was evaluated by fluorescence microscopy . ^^^ After 12 h of culture only HLA DR+ cells ( LC ) expressed CD11a , CD 18 , and ICAM 1 . ^^^ MoAb against CD11a , CD 18 , or ICAM 1 reduced the allostimulatory capacity of LC by greater than 70 % ; combinations of these MoAb reduced proliferation even more ( 90 % ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The result in this study clearly demonstrates that efficient re targeting of human CTL by SE is dependent on a proper interaction with the heterodimer CD11a / CD18 ( Leu CAMa , LFA 1 ) on the CTL and its target cell ligand ICAM 1 . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In order to study the possible role of antigen independent adhesion systems in thyroid autoimmunity , we evaluated by indirect immunofluorescence the expression of lymphocyte functional antigen 1 ( LFA 1 ) and its ligand ICAM 1 on mononuclear cell infiltrates ( when present ) and thyroid follicular cells of four patients with Hashimoto ' s thyroiditis , 30 with Graves ' disease , five with papillary cancer , two with follicular adenoma , and two normal thyroid specimens . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Keratinocyte ICAM 1 ( CD 54 ) and T cell LFA 1 ( CD11a / CD18 ) mediate enhanced lysis of IFN gamma treated keratinocytes . ^^^ Lysis of nontreated KC was blocked by mAb against class 1 or CD 3 , but not by mAb against ICAM 1 or LFA 1 . ^^^ In contrast , lysis of IFN KC was partially inhibited by anti ICAM 1 or anti LFA 1 mAb , but resisted inhibition by anti class 1 mAb except in the presence of anti ICAM 1 . ^^^ These results indicate that both ICAM 1 / LFA 1 and Ag / CD3 TcR interactions are important for Ag specific lysis of IFN KC , whereas lysis of nontreated KC depends on Ag / CD3 TcR but not ICAM 1 / LFA 1 interactions . ^^^ Equivalent inhibition of IFN KC lysis by mAb against ICAM 1 or LFA 1 suggests that ICAM 1 is the only LFA 1 ligand involved in enhanced IFN KC lysis . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Neutrophil endothelial cell interaction is regulated in part by the leukocyte adhesion receptor CD 18 glycoprotein complex and the endothelial intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Up regulation of the LFA 1 pathway occurs in the absence of any detectable surface redistribution of this molecule , suggesting an activation dependent modification leading to a high affinity ICAM 1 binding state . ^^^ The TcR independence of CD 2 adhesion function implies a critical role of the CD 2 pathway in initiating cell cell interactions prior to TcR engagement and LFA 1 ICAM 1 binding and underscores the complementary nature of the CD 2 and LFA 1 adhesion pathways during the immune response . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The conclusion that the mAb WT . 1 and WT . 3 specifically recognize the rat CD11a and CD 18 , respectively , was based on : ( a ) their ability to inhibit homotypic aggregation of splenic concanavalin A ( Con A ) blasts ; ( b ) sodium dodecyl sulfate polyacrylamide gel electrophoretic analysis of the antigens recognized ; ( c ) their ability to inhibit binding of Con A blasts to the purified ligand , namely the ICAM 1 antigen and ( d ) their blocking abilities in mixed leukocyte reaction . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies R15 . 7 ( anti CD 18 ) , R7 . 1 ( anti CD11a , LFA 1 ) , and R6 . 5 ( anti ICAM 1 ) , administered intravenously before C5a exposure , strongly inhibited leukocyte adherence while antibody LM 2 ( anti CD11b , Mac 1 ) produced significant , but weaker , inhibition . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
First , as has been documented by many investigators , ICAM 1 mediates adhesion between ICAM 1 and lymphocyte function associated Ag 1 ( LFA 1 ) bearing cells . ^^^ We have investigated whether this difference is due to LFA 1 mediated signaling , and we present data that demonstrates that although ICAM 1 does not deliver costimulatory signals required for T cell activation , the interaction of LFA 1 with ICAM 1 does synergize with TCR transduced signals . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
A panel of monoclonal antibodies was applied to frozen tissue sections , including anti intercellular adhesion molecule 1 ( ICAM 1 ) , anti lymphocyte function associated antigen 1 ( LFA 1 ) , and anti HLA DR . ^^^ Endothelial cell expression of ICAM 1 and lymphocyte expression of LFA 1 were unaffected over the course of the ensuing , profound marrow aplasia . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Stimulation of B lymphocytes through surface Ig receptors induces LFA 1 and ICAM 1 dependent adhesion . ^^^ Engagement of the surface Ig receptor with anti IgM antibodies stimulates murine B lymphocytes to markedly increase their expression of the cell adhesion molecules ICAM 1 and LFA 1 . ^^^ In all cases , the formation of cell cell conjugates is dependent on LFA 1 ICAM 1 mediated interactions as assessed in mAb blocking experiments . ^^^ Together , these results demonstrate that there is an induction of both ICAM 1 and LFA 1 on stimulated B cells and a corresponding increase in the adhesiveness of these cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Blocking studies revealed that anti CD 3 induced T cell proliferation and T cell LC cluster formation was inhibited by both anti LFA 1 and anti ICAM 1 mAb suggesting that ICAM 1 expressed on LC must bind to LFA 1 on T cells to facilitate proliferative responses of T cells to anti CD 3 mAb . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule 1 ( ICAM 1 ) is an integral membrane protein , a member of the immunoglobulin superfamily , and a ligand for LFA 1 , a beta 2 leukocyte integrin . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To assess the actual involvement of LFA 1 in lymphocyte HE cell interaction at body temperature , we examined effects of newly developed anti rat LFA 1 and anti rat ICAM 1 monoclonal antibody on binding of resting or activated lymphocytes to a rat HE cell line at 37 degrees C . ^^^ We found that ( a ) LFA 1 / ICAM 1 independent pathway was predominant in the interaction between resting lymphocytes and HE cells , indicating that LFA 1 functions little , if any , in the interaction and ( b ) lymphocyte triggering through CD 3 significantly increased binding inducing a LFA 1 / ICAM 1 dependent pathway , whereas phorbol 12 myristate 13 acetate stimulation also enhanced lymphocyte binding but inducing a LFA 1 dependent / ICAM 1 independent pathway . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Regulation of adhesion of ICAM 1 by the cytoplasmic domain of LFA 1 integrin beta subunit . ^^^ Truncation of the cytoplasmic domain of the beta , but not the alpha , subunit of LFA 1 eliminated binding to ICAM 1 and sensitivity to phorbol esters . ^^^ Thus , LFA 1 binding to ICAM 1 was found to be regulated by the cytoplasmic domain of the beta subunit of LFA 1 . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We recently found that mast cells ( MCs ) express leukocyte recognition molecules , including ICAM 1 antigen , a natural ligand of LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Both the integrins LFA 1 and Mac 1 bind to ICAM 1 , an immunoglobulin superfamily member . ^^^ Previously , we localized the binding sites of LFA 1 and the major group of human rhinoviruses to the first NH 2 terminal immunoglobulin like domain of ICAM 1 . ^^^ Here , we show that the binding site on ICAM 1 for Mac 1 is unexpectedly distinct from that for LFA 1 and maps to the third NH 2 terminal immunoglobulin like domain . ^^^ Agents that interfere with carbohydrate processing provide evidence that the size of the N linked oligosaccharide side chains on ICAM 1 affects binding to Mac 1 but not to LFA 1 . ^^^ Thus , we suggest that the extent of glycosylation on ICAM 1 may regulate adhesion to LFA 1 or Mac 1 in vivo . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The percentage of monocytes expression LFA 1 alpha , ICAM 1 and HLA DR was slightly lower in recent onset type 1 diabetes ( n = 13 ) compared to normal subjects ( n = 15 ) and was significantly decreased after activation of cells with lipopolysaccharide and interferon gamma for 5 24 hr . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Using this mAb , LFA 1 dependent binding to both stimulated and unstimulated endothelium was found to be totally accounted for by ICAM 1 and ICAM 2 . ^^^ Homotypic aggregation of an Epstein Barr virus transformed B cell line , JY , was found to be solely ICAM 1 and ICAM 2 dependent , while in the case of the T cell lymphoma cell line , SKW 3 , anti ICAM 2 mAb in conjunction with anti ICAM 1 mAb could not inhibit the LFA 1 dependent aggregation . ^^^ Using a cell binding assay to purified LFA 1 in conjunction with anti ICAM 1 and anti ICAM 2 mAb , we have demonstrated that this putative third ligand for LFA 1 exists on SKW 3 and other cell lines . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule 1 ( ICAM 1 ) is a membrane bound glycoprotein that is a ligand for lymphocyte function associated antigen 1 ( LFA 1 ) and is important for a number of cell adhesions in immune reactions . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
B cell associated LFA 1 and T cell associated ICAM 1 transiently cluster in the area of contact between interacting cells . ^^^ T cell associated LFA 1 does not cluster with any specific pattern , but ICAM 1 does . ^^^ An antibody that recognizes ICAM 1 ( YN / 1 . 7 ) , the ligand for LFA 1 , inhibits conjugation . ^^^ LFA 1 on B cells and ICAM 1 on T cells transiently cluster with similar , albeit not identical , kinetics to the site of cell cell contact . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Expression of the LFA 1 beta 2 integrin ( CD11a / CD18 ) and ICAM 1 ( CD 54 ) in normal and coeliac small bowel mucosa . ^^^ CD11a ( leucocyte function associated antigen 1 , LFA 1 ) is expressed by most T cells , and is involved in antigen presentation by macrophages via its counter receptor , intercellular adhesion molecule ( ICAM 1 , CD 54 ) . ^^^ These data suggest that the LFA 1 / ICAM 1 dependent antigen presentation pathway is of minor importance to IEL in the normal small bowel , and does not assume a major role in coeliac disease . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
When administrated on day 6 after infection , antibody to the leukocyte integrin leukocyte function antigen 1 ( LFA 1 ) but not antibodies to MAC 1 , LECAM 1 ( the MEL 14 antigen ) , alpha 4 integrin or ICAM 1 dramatically reduced the incidence of CM , leading to survival of most mice until the later onset of anemia . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Treatment with monoclonal antibodies ( mAb ) directed against leukocyte function antigen 1 ( LFA 1 , the ligand of ICAM 1 ) on days 6 , 8 and 10 almost totally prevented ECM , while decreasing blood TNF levels . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule 1 ( ICAM 1 ) is found on the surface of many hemopoietic and non hemopoietic cells and can function as an adhesive ligand for the integrin , leukocyte function associated molecule 1 ( LFA 1 , CD11a / CD18 ) . ^^^ ICAM 1 / LFA 1 interaction is thought to be of importance in many immune mediated cell cell adhesion reactions . ^^^ We have compared the ability of ICAM 1 expressed on the surface of human fibroblasts ( FB ) to function as a receptor for HRV as well as a receptor for LFA 1 bearing human T lymphocytes . ^^^ As these two cytokines both up regulate ICAM 1 on the surface of human FB , the contrasting effects of IFN gamma and IL 1 on human FB ICAM 1 adhesion to HRV and to LFA 1 suggest that qualitative as well as quantitative alterations of the ICAM 1 molecule may contribute to its specificity of ligand recognition . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Suppressed expression of ICAM 1 and LFA 1 and abrogation of leukocyte collaboration after exposure of human mononuclear leukocytes to respiratory syncytial virus in vitro . ^^^ These studies were undertaken to investigate effects of exposure and resultant net IL 1 inhibitor activity on the expression of the intercellular adhesion molecule 1 ( ICAM 1 ) , and its ligand the lymphocyte function associated antigen ( LFA 1 ) . ^^^ MNL collected at 1 , 4 , and 24 h after exposure to influenza virus ( which induces net IL 1 bioactivity ) showed enhanced expression of ICAM 1 and LFA 1 relative to sham exposed MNL and exhibited cell clustering . ^^^ In contrast , exposure to RSV was associated with suppressed expression of both ICAM 1 and LFA 1 and with minimal detectable cell clustering throughout the culture period . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To test this hypothesis , antibody against the ligand for LFA 1 , ICAM 1 , was administered during primary immunization with human gamma globulin ( HGG ) and long term secondary antibody responses monitored . ^^^ These findings suggest a role for ICAM 1 / LFA 1 interaction in determining the long term outcome of contact with antigen . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Human intercellular adhesion molecule 1 ( ICAM 1 ) , a specific ligand for the lymphocyte function associated Ag 1 ( LFA 1 ) , plays an important role in leukocyte endothelial cell interactions . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Increased expression of leukocyte function associated antigen 1 ( LFA 1 ) and intercellular adhesion molecule 1 ( ICAM 1 ) by alveolar macrophages of patients with pulmonary sarcoidosis . ^^^ Leukocyte function associated antigen 1 ( LFA 1 ) and its ligand intercellular adhesion molecule 1 ( ICAM 1 ) are cell adhesion molecules that play an important role in the capacity of monoculear phagocytes ( MPs ) to present antigens to T lymphocytes . ^^^ Since in pulmonary sarcoidosis ( PS ) this capacity is increased at sites of disease activity , we studied the expression of LFA 1 and ICAM 1 on peripheral blood monocytes ( BMs ) and alveolar macrophages ( AM ) obtained by bronchoalveolar lavage ( BAL ) from normal subjects ( n = 7 ) and patients with PS ( n = 14 ) . ^^^ To accomplish this , immunocytochemical stainings were made on cytocentrifuge preparations using anti LFA 1 ( anti CD 11a ) and anti ICAM 1 ( anti CD 54 ) monoclonal antibodies ( MoAbs ) . ^^^ Normal and sarcoid BMs displayed a high percentage of positivity with both MoAbs with no difference between study groups ( LFA 1 : control BM 87 . 8 + / 8 . 8 percent ; sarcoid BM 84 . 7 + / 9 . 5 percent ; ICAM 1 : control BM 80 . 8 + / 10 percent ; sarcoid BM 88 . 0 + / 4 . 2 percent ; p = NS for all comparisons ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In the present study , sequential changes in the expression of immune adhesion receptors ( LFA 1 , CD 2 ) and their ligand molecules ( ICAM 1 , LFA 3 ) were studied in heart allografts during rejection . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Enterocyte ICAM 1 was involved in adhesion to activated T cells through binding to the leukocyte function associated antigen 1 ( LFA 1 ) . ^^^ These findings support the hypothesis that lympho epithelial interactions involving the ICAM 1 / LFA 1 pathway may be implicated in immunosurveillance of colon adenocarcinomas , inflammatory bowel disease and celiac disease , where increased levels of proinflammatory cytokines are locally produced within the gut mucosa . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The role of LFA 1 / ICAM 1 interactions during murine T lymphocyte development . ^^^ We have examined the expression and function of the cell adhesion molecules LFA 1 ( CD11a / CD18 ) , ICAM 1 ( CD 54 ) , and ICAM 2 in murine fetal thymic ontogeny and in the adult thymus . ^^^ On fetal days 14 and 15 , 40 to 50 % of thymocytes coexpress high levels of LFA 1 and ICAM 1 , as determined by flow cytometry . ^^^ Although LFA 1 expression remains unchanged thereafter , ICAM 1 expression appears to be differentially regulated in different thymocyte subpopulations , with CD4+8+ cells being ICAM 1lo and CD 4 8 thymocytes remaining ICAM 1hi . ^^^ Addition of anti ICAM 1 or anti LFA 1 antibodies to fetal thymic organ cultures results in the impaired generation of CD4+8+ cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Although there is strong evidence that lymphocyte function associated antigen 1 ( LFA 1 ) positive T cells are retained in the epidermis via intercellular adhesion molecule 1 ( ICAM 1 ) expression induced on keratinocytes , the molecular basis for the directed migration of T cells or neutrophils towards the epidermis is not known . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We have been investigating an interaction involving two of these molecules : lymphocyte function associated antigen 1 ( LFA 1 ) on the T cell and its ligand , intercellular adhesion molecule 1 ( ICAM 1 ) on the APC , in an effort to assess the effect its interruption would have on the ability of the recipient to reject an allograft . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Hydrogen peroxide pretreatment of perfused canine vessels induces ICAM 1 and CD 18 dependent neutrophil adherence . ^^^ H2O2 induced PMN retention was completely inhibited by addition of catalase or the hydroxyl radical scavenger dimethylthiourea to the perfusate by incubation of the PMN with a monoclonal antibody ( Mab ) against CD 18 ( R15 . 7 ) or by perfusion of the H2O2 treated vessel with CL18 / 6 , a Mab against canine ICAM 1 ( intercellular adhesion molecule 1 ) . ^^^ These results indicate that H2O2 in the absence of evidence for permanent endothelial cell injury , can induce a transient , reversible , platelet activating factor dependent adherence of PMNs to vessels by mechanisms that depend on an intact endothelium and involve CD 18 on the PMN and ICAM 1 on the endothelium . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Keratinocyte expression of intercellular adhesion molecule 1 ( ICAM 1 ) correlated with infiltration of lymphocyte function associated antigen 1 ( LFA 1 ) positive cells in evolving allergic contact dermatitis reactions . ^^^ We investigated the kinetics of ICAM 1 expression on keratinocytes and correlated this with the numbers of lymphocytes expressing LFA 1 in the dermis and epidermis of evolving allergic contact dermatitis reactions . ^^^ The LFA 1 cells were observed to be in close proximity to keratinocytes expressing ICAM 1 , thus supporting the hypothesis that T lymphocytes attach to keratinocytes via LFA 1 / ICAM 1 molecules . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
A circulating form of the usually membrane bound intercellular adhesion molecule 1 ( ICAM 1 ) was identified and characterized in normal human serum , and in sera from patients with leukocyte adhesion deficiency ( LAD ) . ^^^ The elevated levels of cICAM 1 in LAD sera may be due to an inability to adsorb cICAM 1 to cell bound LFA 1 or may be an indirect result of the pathology accompanying the syndrome . cICAM 1 bound to mAb specific for four distinct ICAM 1 epitopes localized in domains D 1 , D 2 , D 4 , and D 5 , and displayed similar molecular size properties as recombinant soluble ICAM 1 on FPLC size exclusion chromatography . ^^^ These data indicate that cICAM 1 contains most , if not all , of the five extracellular domains of membrane ICAM 1 , as well as the ability to bind specifically to LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We have defined the regions of the cytoplasmic domain of the leukocyte integrin lymphocyte function associated antigen 1 ( LFA 1 ) that are required for active binding of its extracellular domain to intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^ Furthermore , multiple mutations demonstrate that serine phosphorylation can be dissociated from phorbol ester stimulated binding of LFA 1 to ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We examined the role of intracellular adhesion molecule 1 ( ICAM 1 or CD 54 ) in the development of pulmonary edema in rabbits after pulmonary artery occlusion and reperfusion using a monoclonal antibody ( MAb ) RR1 / 1 directed against ICAM 1 , a ligand for the CD 18 leukocyte adhesion glycoprotein complex . ^^^ MAbs RR1 / 1 ( 0 . 5 mg / kg ) or IB 4 ( 1 . 0 mg / kg ) ( MAb directed against an epitope on the CD 18 adhesion glycoprotein ) was infused 45 60 min before the start of reperfusion to assess the roles of ICAM 1 and CD 18 in the response . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
A useful model for analysis of the interaction of the leucocyte integrin LFA 1 with ICAM 1 . ^^^ The adhesion of human T lymphoblasts to ICAM 1 expressing normal dermal fibroblasts has been assessed as a sensitive model system for the analysis of the interaction of the leucocyte integrin LFA 1 with its counter receptor ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The upregulated ICAM 1 expression in the allograft may accelerate graft rejection by augmenting adhesiveness of LFA 1 positive graft infiltrating cells . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Adherence of neutrophils to cytokine stimulated neural cells is mediated primarily by ICAM 1 : LFA 1 interactions , because 70 % to 90 % of the binding can be blocked by monoclonal antibodies to either ligand . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
However , T dependent signalling of B cells was profoundly inhibited by mAb to CD 18 ( beta chain of LFA 1 ) on the T cell or CD 54 ( ICAM 1 ) on the B cell , demonstrating the importance of this pair of adhesion molecules in early T B cell interactions . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
These cells have upregulated many adhesion molecule systems [ e . g . , Pgp 1 , LFA 1 and ICAM 1 ( Swain unpublished ) ] , but downregulated the Mel 14 homing receptor . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Expression of the leucocyte integrin LFA 1 ( CD11a / CD18 ) and its ligand ICAM 1 ( CD 54 ) in lymphoid malignancies is related to lineage derivation and stage of differentiation but not to tumor grade . ^^^ The leucocyte adhesion molecule LFA 1 ( CD11a / CD18 ) and its counter structure ICAM 1 ( CD 54 ) play a pivotal role in cell cell interactions in the immune system and hence their expression on malignant cells might play an important role in determining the biological behavior of lymphoid malignancies . ^^^ This study examined the LFA 1 ( CD11a / CD18 ) and ICAM 1 ( CD 54 ) expression profiles of a large series of non Hodgkin ' s lymphomas ( NHL , n = 220 ) and lymphoid leukemias ( LL , n = 48 ) , which , by their differentiation antigen phenotype represented essentially all stages of lymphoid development from stem cell to mature activated T and B lymphocyte . ^^^ It was found that NHL and LL differentially express LFA 1 and ICAM 1 molecules according to their lineage derivation , stage of differentiation , and growth pattern . ^^^ Specifically : ( a ) T cell neoplasms nearly always express LFA 1 whereas B cell tumors are often LFA 1 low / negative ; ( b ) ICAM 1 expression is largely confined to tumors with a mature or activated T or B cell phenotype ; ( c ) neoplasms with a leukemic dissemination pattern are either ICAM 1 low or negative . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Antibodies to ICAM 1 , LFA 1 , CD 4 , and MHC class 2 were without effect . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Homotypic cell aggregations of human myeloma cells with ICAM 1 and LFA 1 molecules . ^^^ In order to clarify the surface molecules involved in homotypic cell aggregations and physiological roles of these cell aggregations , we investigated the expressions of intercellular adhesion molecule 1 ( ICAM 1 ) and lymphocyte function associated antigen 1 ( LFA 1 ) on 20 samples of freshly isolated myeloma cells and three myeloma cell lines and the effect of anti ICAM 1 and anti LFA 1 alpha antibodies on myeloma cell proliferation in vitro . ^^^ These spontaneous cell aggregations were completely released by adding either anti ICAM 1 or anti LFA 1 alpha antibody . ^^^ During short term culture , spontaneous proliferation of myeloma cells in vitro and their proliferative responses to recombinant interleukin 6 ( rIL 6 ) were not affected by pretreatment of myeloma cells with anti ICAM 1 or anti LFA 1 alpha antibody . ^^^ Therefore these data suggest that homotypic cell aggregation of myeloma cells is mediated by ICAM 1 and LFA 1 molecules , but myeloma cell proliferation may not be modulated by these adhesion molecules during short term cultures . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Antibodies against LFA 1 and ICAM 1 inhibited binding , directly implicated these molecules in T cell PMA induced U 937 adhesion . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Thymocyte LFA 1 and thymic epithelial cell ICAM 1 molecules mediate binding of activated human thymocytes to thymic epithelial cells . ^^^ We found that activated mature and immature thymocytes bound maximally at 37 degrees C to IFN gamma treated ICAM 1+ and HLA DR+ TE cells and this TE activated thymocyte binding was inhibited by antibodies to LFA 1 alpha chain ( CD11a ) ( 68 . 1 + / 5 . 6 % inhibition , p less than 0 . 01 ) and ICAM 1 ( 73 . 9 + / 7 . 7 % inhibition , p less than 0 . 05 ) . ^^^ As with antibodies to LFA 3 and CD 2 , antibodies to LFA 1 and ICAM 1 also inhibited PHA induced mature thymocyte activation when accessory signals were provided by TE cells in vitro . ^^^ Finally , LFA 1 and ICAM 1 were expressed early on in human thymic fetal ontogeny in patterns similar to those seen in postnatal thymus . ^^^ Taken together , these data suggest that resting mature and immature thymocytes bind to TE cells via the CD2 / LFA 3 ligand pair , whereas activated thymocytes bind via both CD2 / LFA 3 and LFA 1 / ICAM 1 ligand systems . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Lymphocyte function associated antigens 1 and 3 ( LFA 1 , LFA 3 ) and intercellular adhesion molecule 1 ( ICAM 1 ) are cell surface adhesion molecules necessary for immune processes requiring intercellular contact . ^^^ It was recently proposed that malignant Burkitt ' s lymphoma cells ( BL ) may escape from immunosurveillance through the downregulation of LFA 1 ( CD11a / CD18 ) or LFA 3 ( CD 58 ) and ICAM 1 ( CD 54 ) molecules . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Moreover , cell adhesion triggered through VLA 4 can not be inhibited by cell preincubation with anti LFA 1 alpha ( CD11a ) , LFA 1 beta ( CD 18 ) , or ICAM 1 ( CD 54 ) mAb as opposed to that mediated by phorbol esters , indicating that it is a LFA 1 / ICAM 1 independent process . ^^^ An alternative leukocyte homotypic adhesion mechanism , LFA 1 / ICAM 1 independent , triggered through the human VLA 4 integrin . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We have shown that human thymic epithelial ( TE ) cells produce IL 1 alpha , IL 1 beta , and TE cells bind to thymocytes by CD 2 and LFA 1 molecules on thymocytes and LFA 3 , ICAM 1 on TE cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Monocyte adhesion induced by anti CD 14 mAb required neither Fcy gamma R engagement nor cross linking of CD 14 , because adhesion was induced by F ( ab ) ' 2 fragments , as well as by monovalent F ( ab ) fragments of anti CD 14 mAb . mAb to CD11a , CD 18 , and intercellular adhesion molecule 1 ( ICAM 1 ) , but not antibodies to CD11b and CD11c , inhibited monocyte adhesion induced by CD 14 engagement . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Although the fibroblasts did not express the ligand Mala 2 , the murine homologue of human ICAM 1 , a monoclonal antibody against LFA 1 , its cognate receptor on the lymphocytes , nevertheless effectively inhibited binding . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The results indicate that antibodies against CD 54 ( intercellular adhesion molecule 1 , ICAM 1 ) , CD11a ( leucocyte function antigen 1 , LFA 1 , alpha chain ) , and CD 18 ( common beta chain of the beta 2 integrin family ) significantly ( by 50 % ) reduce the binding of LGL onto inhibitory target cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Pretreatment of T lymphocytes with monoclonal antibodies directed at either CD11a / CD18 ( LFA 1 ) or CD 2 molecules or that of EC with monoclonal antibodies directed at either CD 54 ( ICAM 1 ) or CD 58 ( LFA 3 ) molecules significantly inhibited T lymphocyte induced enhancement in endothelial permeability , thus indicating that activated T lymphocytes utilize both type 1 ( CD11a / CD18CD54 ) and type 2 ( CD2CD58 ) intercellular adhesion pathways to augment endothelial permeability and signals received via CD 3 or CD 28 molecules on T lymphocytes further enhance this process . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The molecules studied included CD 3 , CD45R , UCHL 1 ( CD45RO ) , lymphocyte function associated antigen 1 ( LFA 1 ) ( CD11a , CD 18 ) , intercellular adhesion molecule 1 ( ICAM 1 ) ( CD 54 ) , 4B4 ( CD 29 ) , CD 44 , CD 2 , and LFA 3 ( CD 58 ) . ^^^ Antibodies to LFA 1 alpha and beta and ICAM 1 could significantly block the binding of lymphokine activated killer ( LAK ) cells or TIL to human glioblastoma cells suggesting that these molecules play a role in the binding and subsequent migration of lymphocytes into brain tumor tissue . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies against lymphocyte function associated molecule LFA 1 and its ligand , intercellular adhesion molecule 1 ( ICAM 1 ) , and the addressin MECA 325 , a marker of mouse lymph node high endothelial venules , were tested on frozen sections in combination with the avidin biotin complex technique . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The ability of fixed ThCD 3 to induce entry of B cell into cycle is not due to an increase in expression of CD 3 , CD 4 , LFA 1 , ICAM 1 , class 1 MHC or Thy 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
CD27+ B lymphocytes are characterized by the following criteria : ( a ) in terms of physical properties , the CD27+ B cells form a population with an increased cell size combined with a decreased cell density ; ( b ) the CD 27 expression of tonsillar B lymphocytes is postively correlated with mIgA but negatively correlated with membrane IgM / membrane IgD positivity ; ( c ) CD 27 on B cells can be induced selectively by the combination of Staphylococcus aureus plus interleukin 2 , but not by either treatment alone , and ( d ) CD27+ B lymphocytes express high levels of the adhesion structures LFA 1 ( CD11a ) , ICAM 1 ( CD 54 ) , LFA 3 ( CD 58 ) and of the lymphocyte homing receptor CD 44 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Target lysis by human LAK cells is critically dependent upon target binding properties , but LFA 1 , LFA 3 and ICAM 1 are not the major adhesion ligands on targets . ^^^ The goal of this study was : ( a ) to examine the correlation between binding properties and susceptibility to lysis of 6 informative target cell lines ; ( b ) to evaluate the role , as ligands on these targets , of adhesion molecules such as LFA 1 , LFA 3 and ICAM 1 . ^^^ The differences in the expression of the classical cell cell adhesion molecules LFA 1 , LFA 3 and ICAM 1 on the target surfaces were only marginal , insufficient to explain the striking differences in susceptibility to lysis and in binding properties . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Blocking mAb directed to either CD11a / CD18 ( LFA 1 , a ICAM 1 counter receptor ) , CD11b / CD18 ( Mo 1 ) , or CD 18 ( common beta 2 integrin ) also blocked greater than 90 % of PMN transmigration . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
LFA 3 , a ligand for CD 2 , but not ICAM 1 , a ligand for LFA 1 , was , expressed on glioma cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To elucidate mechanisms governing cornea leukocyte interactions , cultured human CE cells and intact corneas were examined for expression of intercellular adhesion molecule 1 ( ICAM 1 ) , which binds the lymphocyte function associated antigen 1 ( LFA 1 ) on all leukocytes and enhances delayed type hypersensitivity mediated by class 2 major histocompatibility complex antigens . ^^^ Standard leukocyte adherence assays demonstrated ICAM 1 mediated CE neutrophil binding , which was specifically blocked by antibody to ICAM 1 or antibodies to LFA 1 on neutrophils . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Peripheral blood T cells activated by cross linking of the CD3 / T cell receptor complex , which increased the expression of cell adhesion molecules LFA 1 , LFA 3 and ICAM 1 , induced the proliferation of autologous T cell clones . ^^^ As CD 2 is the natural ligand for LFA 3 , increased expression of T cell surface adhesion molecules LFA 1 , ICAM 1 and particularly LFA 3 during an inflammatory response may rapidly recruit T cells that are activated through the CD 2 pathway . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Since the initiation of a specific immune response also involves antigen receptor independent interactions between accessory molecules , such as lymphocyte function associated antigen 1 ( LFA 1 ) with intercellular adhesion molecule 1 ( ICAM 1 ) and lymphocyte function associated antigen 3 ( LFA 3 ) with CD 2 , it was of interest to determine whether TEC can express the adhesion molecules ( ICAM 1 and LFA 3 ) which augment the efficiency of antigen presentation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Instead , antibodies to the LFA 1 ( CD11a / CD18 ) intercellular adhesion molecule 1 ( ICAM 1 ) ( CD 54 ) adhesion system reduce inhibition . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The complementary adhesion molecules LFA 1 ( CD11a , 18 ) / ICAM 1 ( CD 54 ) and LFA 2 ( CD 2 ) / LFA 3 ( CD 58 ) have been shown to be important in T cell interaction with lymphoid target cells . ^^^ Effector cells expressed LFA 1 and LFA 2 but little or no ICAM 1 and LFA 3 . ^^^ Monoclonal antibody against ICAM 1 or LFA 1 produced the greatest inhibition of HKC lysis , and their effects were not additive . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Biopsies were taken from 48 h patch test reactions to six structurally unrelated irritants , and frozen sections immunolabeled with monoclonal antibodies to the major histocompatibility complex class 2 antigen , HLA DR , intercellular adhesion molecule 1 ( ICAM 1 ) , and the 88 Kd glycoprotein CD 36 ( OKM 5 ) , as well as to the CD 3 ( T cells ) and CD11a ( lymphocyte function associated antigen 1 , LFA 1 ) antigens . ^^^ In contrast , keratinocytes showed extensive upregulation of ICAM 1 , with an excellent spatial association between ICAM 1 expression and LFA 1 positive leucocytes in the epidermis . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Addition of monoclonal antibodies 84H10 directed against ICAM 1 and MHM 23 directed against the common beta subunit of LFA 1 ( CD 18 ) resulted in greater than 50 % suppression of syncytia formation in cultures of these clones infected with cell free virus . ^^^ However , anti ICAM 1 and anti LFA 1 ( CD 18 ) antibodies did not reverse the TNF alpha induced enhancement of syncytia formation of HIV 1 infected clones 1B11 39 and 17D 9 . ^^^ Re evaluation of the involvement of the adhesion molecules ICAM 1 / LFA 1 in syncytia formation of HIV 1 infected subclones of a CEM T cell leukemic line . ^^^ The role of the adhesion molecules intercellular adhesion molecule 1 ( ICAM 1 ) and LFA 1 in human immunodeficiency virus type 1 ( HIV 1 ) induced cell fusion was investigated in subclones of a T cell leukemic cell line ( CEM ) with differing abilities to form syncytia . ^^^ The expression of ICAM 1 but not LFA 1 was upregulated on the clones following treatment with interferon gamma ( IFN gamma ) ; however , this did not overcome the delay in syncytia formation observed in these cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Remarkably , however , expression of a ligand of LFA 1 , intercellular adhesion molecule 1 ( ICAM 1 ; CD 54 ) , was enhanced with similar kinetics as CR 3 and p 150 , 95 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Since the interaction thymic epithelial cells ( ICAM 1 and LFA 3 positive ) / thymocytes ( LFA 1 and CD 2 positive ) has been shown to be necessary for promotion of activation and maturation of thymocytes , the interaction keratinocytes ( ICAM 1 and LFA 3 positive , as we demonstrated ) / T lymphocytes ( LFA 1 and CD 2 positive as well ) might be , by analogy , important not only for the `` homing ' ' of T lymphocytes within the epidermis , but also for the epidermis being considered a peripheral inductive site for T cell activation and maturation . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We found that FDC express high levels of the adhesion receptors intercellular adhesion molecule 1 ( ICAM 1 [ CD 54 ] ) and vascular cell adhesion molecule 1 ( VCAM 1 ) , while the B lymphocytes express lymphocyte function associated antigen 1 ( LFA 1 [ CD11a / 18 ] ) , very late antigen 4 ( VLA 4 [ CD49d ] , and CD 44 . ^^^ Furthermore , we established that both the LFA 1 / ICAM 1 and VLA 4 / VCAM 1 adhesion pathways are involved in FDC B lymphocyte binding , and therefore , these pathways might be essential in affinity selection of B cells and in the formation of B memory cells . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The LFA 1 pathway was found to play a key role in T cells blood DC adhesion ; monoclonal antibodies ( mAb ) against both LFA 1 and ICAM 1 strongly inhibited adhesion between those cells . ^^^ In contrast to the strong inhibition by antibodies to LFA 1 and ICAM 1 , antibodies to CD 44 enhanced conjugate formation between T cells and blood DC . ^^^ Together , our results show that the LFA 1 / ICAM 1 pathway plays a central role in T cell blood DC adhesion , a situation like that in T cell adhesion to lymphoid DC . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Adhesion of resting or activated neutrophils through the integrins LFA 1 and Mac 1 to ICAM 1 in a lipid bilayer does not occur at physiologic shear stresses ; however , static incubation of activated neutrophils allows development of adhesion that is greater than 100 fold more shear resistant than found on CD 62 . ^^^ Addition of a chemoattractant to activate LFA 1 and Mac 1 results in the arrest of neutrophils rolling on bilayers containing both CD 62 and ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We have systematically analyzed the role of four receptor / ligand interactions that mediate adhesion of peripheral human CD4+ T cells to cultured human umbilical vein endothelial cells ( HUVEC ) : T cell LFA 1 binding to ICAM 1 and an alternative ligand ( `` ICAM 10 ' ' ) , T cell VLA 4 binding to VCAM 1 , and T cell binding to ELAM 1 . ^^^ LFA 1 adhesion dominates with PMA activated T cells but the strength and predominant LFA 1 ligand is determined by the activation state of the HUVEC ; while ICAM 1 is the dominant ligand on IL 1 induced HUVEC , `` ICAM 10 ' ' dominates binding to uninduced HUVEC . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The cytotoxicity was major histocompatibility complex class 2 restricted and inhibited by antibodies to ICAM 1 and LFA 1 and not by antibodies to tumor necrosis factor alpha , lymphotoxin , and gamma interferon . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Neutrophil adhesion to interleukin 1 ( IL 1 ) stimulated human umbilical vein endothelial cells ( HUVEC ) involves the CD 18 family of leukocyte integrins ( lymphocyte function associated antigen 1 [ LFA 1 ] , Mac 1 , and p 150 , 95 ) and LECAM 1 ( DREG 56 / LEU 8 / LAM 1 antigen ) on neutrophils and intercellular adhesion molecule 1 ( ICAM 1 ) and endothelial leukocyte adhesion molecule 1 ( ELAM 1 ) on the endothelium . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule 1 ( ICAM 1 ) , a ligand of leukocyte function associated antigen 1 ( LFA 1 ) , is present on many cells , including monocyte / macrophages . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
All tested blast populations expressed CD18 / CD11a complex [ leukocyte function antigen 1 ( LFA 1 ) ] and CD 29 ( very late antigen ( VLA ) ) and the majority were positive for CD 54 [ intercellular adhesion molecule 1 ( ICAM 1 ) , 78 . 6 % ] and CD 56 [ neural cell adhesion molecule ( NCAM ) , 64 . 3 % ] . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Motility and ultrastructure of large granular lymphocytes on lipid bilayers reconstituted with adhesion receptors LFA 1 , ICAM 1 , and two isoforms of LFA 3 . ^^^ Here we have studied the motility and ultrastructure of large granule lymphocyte ( LGL ) on lipid bilayers containing purified LFA 1 , ICAM 1 , and the transmembrane and glycophosphatidylinositol isoforms of LFA 3 . ^^^ LGLs moved at 8 microns / min on ICAM 1 but poorly ( less than 1 microns / min ) on its receptor pair LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Remote T cell co stimulation via LFA 1 / ICAM 1 and CD2 / LFA 3 : demonstration with immobilized ligand / mAb and implication in monocyte mediated co stimulation . ^^^ Our studies now demonstrate that co stimulation by the monocytes is dependent on each of two receptor / ligand pathways CD2 / LFA 3 and LFA 1 / ICAM 1 since it is inhibited by each relevant mAb but not a variety of control mAb . ^^^ The hypotheses that CD 2 and LFA 1 could each mediate co stimulation was tested in simplified model systems in which the monocyte was replaced with immobilized CD 2 mAb or purified ICAM 1 presented on a separate surface from the CD 3 mAb . ^^^ Thus : ( a ) the accessory cell function of stimulated fixed monocytes in T cell proliferation requires both the LFA 1 / ICAM 1 and CD2 / LFA 3 pathways ; and ( b ) the T cell molecules CD 2 and LFA 1 can give co stimulatory signals that can act in a `` remote ' ' fashion . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Furthermore , the use of agents that interfere with intracellular signaling , a protein kinase C inhibitor , cAMP analogue , or the combination of a phosphodiesterase inhibitor and adenyl cyclase activator , did not affect the binding of COS cells expressing LFA 1 to purified ICAM 1 . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
MAb against intercellular adhesion molecule type 1 ( ICAM 1 ) and LFA 1 also prevented binding of T lymphocytes , but had a more marked effect on adhesion to SSc fibroblasts than to normal fibroblasts ; they also completely abolished the increased binding to fibroblasts treated with interleukin 1 alpha , tumor necrosis factor alpha , and interferon gamma . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In lesions characterized by strong delayed type hypersensitivity against Mycobacterium leprae ( tuberculoid , reversal reaction , and Mitsuda reaction ) , the overlying epidermis exhibited pronounced keratinocyte intracellular adhesion molecule 1 ( ICAM 1 ) expression and contained lymphocytes expressing the ICAM 1 ligand , LFA 1 . ^^^ Antibodies to LFA 1 and ICAM 1 blocked the response of both alpha beta and gamma delta T cell clones in vitro to mycobacteria . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Antibodies against the homing receptor LECAM 1 ( lectin related leucocyte endothelial cell adhesion molecule 1 ) , alpha 4 integrins , vascular addressins , LFA 1 , or ICAM 1 known to block lymphocyte interaction with particular types of HEV were unable to inhibit the basal adhesion to TME cells , indicating that a further binding mechanism in mice is displayed by this cell type . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Adhesion was markedly inhibited by MAb against CD 18 and CD11b , with lesser or no inhibition being afforded by MAb against CD11a , CD11c , or ICAM 1 . ^^^ These actions were blocked by actinomycin D or cycloheximide and by MAb against HMC ICAM 1 or phagocyte CD 18 , CD11a , or CD11b , suggesting that cytokines provoked adhesion by inducing HMC ICAM 1 synthesis . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
ICAM 1 ( CD 54 ) in 16 patients , and beta 2 integrins ( CD 18 ) in eight patients . beta 1 integrins ( CD 29 ) were expressed in all patients . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The adhesion of T cells to IL 1 activated EC was markedly , but not completely , inhibited by mAb to VCAM 1 as well as to its counter receptor , VLA 4 , whereas , T cell binding to IL 1 activated EC was not blocked by mAb to ICAM 1 or to its counter receptor , LFA 1 . ^^^ In contrast , LFA 1 / ICAM 1 , but not VLA 4 / VCAM 1 , mediated much , but not all , of the binding of T cells to unstimulated EC . ^^^ Moreover , the binding of activated T cells to EC was blocked to a lesser degree by mAb to LFA 1 than that of resting T cells , and mAb to ICAM 1 blocked binding only modestly . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The costimulation mediated by the activation dependent interaction of the VLA 4 and LFA 1 integrins with their respective ligands VCAM 1 and ICAM 1 leads to increased IL 2R alpha ( CD 25 ) expression and proliferation in both CD45RA+ CD4+ and CD45RO+ CD4+ T cells . ^^^ These findings imply that adhesion per se is not sufficient for costimulation , but rather that the costimulation conferred by the VLA 4 / VCAM 1 and LFA 1 / ICAM 1 interactions reflects specialized accessory functions of these integrin pathways . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The CD11a / CD18 ( LFA 1 ) leukocyte integrin receptor mediates homotypic and heterotypic leukocyte adhesion by binding to one of two defined ligands , ICAM 1 or 2 , on the conjugate cell . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
A monoclonal antibody ( mAb ) was raised that , in combination with blocking mAb to ICAM 1 and ICAM 2 , can completely inhibit binding of these cell lines to purified LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Since OKT 3 clustering is known to be dependent on the LFA 1 / ICAM 1 adhesion system , the effect of monoclonal antibodies directed against these molecules was tested . ^^^ Increased expression of LFA 1 or ICAM 1 on CVI patients ' T cells could not be demonstrated . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The role of cell adhesion molecules ( CAM ) LFA 1 , ICAM 1 , LFA 3 , VLA 1 , VLA 4 , CD 29 , CD 44 , and CD 56 in tumor infiltrating lymphocyte ( TIL ) and natural killer cell ( NK ) mediated killing of target cells was studied . ^^^ The melanoma cell lines analyzed were all positive for CD 29 and LFA 3 expression , negative for LFA 1 expression , but showed variable expression of ICAM 1 , VLA 1 , VLA 4 , CD 44 , and CD 56 . ^^^ The effects of anti CAM antibodies on TIL mediated melanoma killing fell into three categories : ( 1 ) consistent inhibition of TIL mediated killing was observed when melanoma cells were pretreated with anti ICAM 1 and anti LFA 3 MoAb or when TIL were pretreated with anti LFA 1 ; ( 2 ) no effect was observed when melanoma cells were pretreated with anti CD 56 ; or ( 3 ) a discreet , but significant , inhibition was observed when target cells were pretreated with anti CD 29 , anti VLA 1 , anti VLA 4 , and anti CD 44 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Antibodies against LFA 1 and ICAM 1 are capable to suppress the GC induced IgE and IgG synthesis of PBMC . ^^^ Furthermore , the monocyte expression of lymphocyte function antigen 1 ( LFA 1 ) , intercellular adhesion molecule 1 ( ICAM 1 ) and HLA DR is modulated by GC stimulation . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Adhesion induced by suboptimal stimulation through these surface Ag or by PMA was mediated primarily through LFA 1 and ICAM 1 . ^^^ Furthermore , adhesion was mediated by at least two distinct cell surface adhesion receptors : LFA 1 / ICAM 1 and a heretofore unknown adhesion receptor . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We established that the very late antigen 4 [ VLA 4 ( CD49d ) ] and the vascular cell adhesion molecule 1 ( VCAM 1 ) are important mediators of binding to synovial endothelium of resting and , to a greater extent , of activated T lymphocytes , whereas the leukocyte function associated antigen 1 [ LFA 1 ( CD11a / 18 ) ] / intercellular adhesion molecule 1 [ ICAM 1 ( CD 54 ) ] pathway is less important in this interaction . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
HIV 1 infection of target monocytes or T cells by cell free virus was blocked completely or partially by some mAb that prevent cell cell interactions ( CD 4 , HLA DR , LFA 1 , LFA 3 ) , but not by others ( ICAM 1 , MAC 1 , gp150 . 95 , CD 2 , CD 3 , CD 14 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The intercellular adhesion molecules ICAM 1 and ICAM 2 are the cell surface ligands for the lymphocyte function associated antigen LFA 1 ( CD11a / CD18 ) and are thought to mediate cell cell adhesion interactions required by the immune system . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
ICAM 1 , the specific ligand of LFA 1 , normally only expressed by endothelial cells , showed a focal expression on basal keratinocytes at sites of intramucosal T cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
All were examined for the expression of LFA 3 , ICAM 1 and its receptor LFA 1 , and HLA DR . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Flow cytometric data showed that the ability of TGF beta to interfere with the formation of Th B cell conjugates was not due to decreased expression of IgD , IgM , class 1 , class 2 , LFA 1 , or ICAM 1 by the B cell . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Induction of this hyporesponsiveness was not due to altered expression of class 2 MHC gene products on the APC but was associated with markedly reduced T lymphocyte APC adhesive interactions despite the lack of a detectable immunophenotypic change in lymphocyte function associated Ag 1 ( LFA 1 ) and intercellular adhesion molecule 1 ( ICAM 1 ) expression on the modified APC . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Monocytes from CFS displayed increased density ( as determined by mean fluorescence channel numbers ) of intercellular adhesion molecule 1 ( ICAM 1 ) and lymphocyte function associated antigen 1 ( LFA 1 ) , but showed decreased enhancing response to recombinant interferon gamma in vitro . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The aggregation was not influenced by mAb against accessory molecules previously shown to be involved directly or indirectly in homotypic aggregation [ CD11a ( LFA 1 ) / CD18 / CD54 ( ICAM 1 ) , CD 58 ( LFA 3 ) / CD2 , BB1 / CD28 , CD 43 , and CD 44 ] . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Transendothelial migration of neutrophils in most settings both in vitro and in vivo appears to require CD 18 integrins on the neutrophil and ICAM 1 on the endothelial cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
LFA 1 can bind to ICAM 1 / ICAM 2 ; and VLA 4 , to VCAM 1 on endothelium . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The possible role ( s ) of the adhesion molecules LFA 1 alpha ( CD11a ) , LFA 1 beta ( CD 18 ) , ICAM 1 ( CD 54 ) , CD 2 ( T 11 , LFA 2 ) , and LFA 3 ( CD 58 ) in the in vitro activation of nickel specific peripheral blood ( PB ) T lymphocytes was studied . ^^^ In those patients who showed no inhibition when anti LFA 1 ( alpha and beta ) MoAb were added , no inhibition was also observed when a mixture of anti LFA 1 ( alpha and beta ) and ICAM 1 MoAb were added to the cultures . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Adhesion molecules such as LFA 1 and ICAM 1 have a role in this homing . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The expression of intercellular adhesion molecule 1 ( ICAM 1 ) , lymphocyte function associated antigen ( LFA 1 ) , and HLA DR molecules was studied in 13 proliferative and 10 secretory endometria as well as cultures of endometrial glands and stroma by avidin biotin complex ( ABC ) procedure using monoclonal antibodies . ^^^ The high level of expression of ICAM 1 , LFA 1 , and HLA DR molecules in human endometrial constituents may contribute to the presence , aggregation , and preferential distribution of lymphoid cells in human endometrium . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
ICAM 1 is a member of the immunoglobulin supergene family and is a ligand for lymphocyte function associated antigen 1 ( LFA 1 ) ; these ICAM 1 / LFA 1 interactions are critical to many cell adhesion processes involved in the immunological response . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Because ICAM 1 is a ligand for the adhesive molecule LFA 1 , which is expressed on the surface of leukocytes and , especially , lymphocytes , the ICAM 1 ( LC ) / LFA 1 ( T lymphocytes ) interactions might , enabling these cells to adhere together , deliver activation signals to T cells , thus constituting the initial event in the generation of a cutaneous immune response . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The arrangement of the immunoglobulin like domains of ICAM 1 and the binding sites for LFA 1 and rhinovirus . ^^^ Intercellular adhesion molecule 1 ( ICAM 1 , CD 54 ) binds to the integrin LFA 1 ( CD11a / CD18 ) , promoting cell adhesion in immune and inflammatory reactions . ^^^ ICAM 1 sequences important to binding LFA 1 , rhinovirus , and four monoclonal antibodies were identified through the characterization of chimeric ICAM 1 molecules and mutants . ^^^ Domain 1 of ICAM 1 contains the primary site of contact for both LFA 1 and rhinovirus ; the presence of domains 3 5 markedly affects the accessibility of the binding site for rhinovirus and less so for LFA 1 . ^^^ Our analysis suggests that rhinoviruses mimic LFA 1 in binding to the most membrane distal , and thus most accessible , site of ICAM 1 . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Human intercellular adhesion molecule 1 ( ICAM 1 ) is a cell surface glycoprotein that serves as one of the major ligands for lymphocyte function associated antigen 1 ( LFA 1 ) , a member of the integrin supergene family of adhesion molecules that is involved in cell cell adhesion . ^^^ Homotypic and heterotypic conjugate formation between leukocytes and between leukocytes and target cells via the LFA 1 / ICAM 1 interaction has been demonstrated to be a critical event in numerous immunologic and inflammatory processes . ^^^ While LFA 1 is expressed by all leukocytes , ICAM 1 is not normally expressed by all tissues with which leukocytes interact , but ICAM 1 may be induced de novo by various cytokines , including interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Cryostat sections of human central nervous system ( CNS ) tissue samples from 10 cases of multiple sclerosis ( MS ) , 11 cases with inflammation and necrosis , and 24 normal controls were immunostained with antibodies to intercellular adhesion molecule 1 ( ICAM 1 ) and its integrin ligand lymphocyte function associated molecule 1 ( LFA 1 ) . ^^^ Thus , CNS vessel ICAM 1 expression is variable in amount in postmortem samples of normal human CNS tissue , may increase early and focally in cellular immune reactions , and , via binding of LFA 1 , may promote leukocyte influx into the CNS . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Functional involvement of the LFA 1 / ICAM 1 adhesion system in the autologous mixed lymphocyte reaction . ^^^ The integrin surface molecule termed lymphocyte functional antigen 1 ( LFA 1 ) , and its physiological ligand intercellular adhesion molecule 1 ( ICAM 1 ) , have been proven to play a relevant role in several immune reactions where cell to cell contact is required : these reactions include allogeneic mixed lymphocyte reaction ( MLR ) and direct cytotoxicity . ^^^ In the present study , we show that monoclonal antibodies ( mAbs ) directed to LFA 1 as well as to ICAM 1 molecules are able to inhibit T cell proliferation in autologous MLR ( AMLR ) . ^^^ It is noteworthy that the LFA 1 molecule is largely represented on the T cell surface , whereas ICAM 1 is poorly expressed on resting T cells : autologous stimulation slightly increases ICAM 1 expression . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In contrast , the specific mean fluorescence for LFA 1 alpha , p 150 , 95 alpha , ICAM 1 , and HLA DR was significantly elevated for CE compared with PBE . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
With the use of blocking monoclonal antibodies , we determined that intercellular adhesion molecule 1 ( ICAM 1 ) and its T cell ligand , lymphocyte function associated antigen 1 ( LFA 1 ) are the major , if not only ligands involved in this system . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We have also demonstrated binding of activated lymphocytes to SGHTL cells : this interaction is increased following treatment with these cytokines and is inhibited by monoclonal antibodies directed against ICAM 1 or lymphocyte function associated antigen 1 ( LFA 1 ) but not by antibodies against CD 2 or MHC class 2 antigens . ^^^ Hence , we conclude that the binding of lymphoblasts to human thyroid cells involves an LFA 1 and ICAM 1 dependent pathway as well as other basal and cytokine inducible pathway ( s ) . ^^^ These do not appear to involve MHC class 2 antigens , CD 2 or an LFA 1 ligand other than ICAM 1 . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Distribution of LCA protein subspecies and the cellular adhesion molecules LFA 1 , ICAM 1 and p 150 , 95 within human foetal thymus . ^^^ The distribution of leucocyte common antigen ( LCA ) protein subspecies and the cellular adhesion molecules LFA 1 ( CD11a ) , ICAM 1 ( CD 54 ) and p 150 , 95 ( CD11c ) has been established within frozen sections of human foetal thymus . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule 1 ( ICAM 1 ) has recently been identified as one of the ligands for lymphocyte function associated antigen 1 ( LFA 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule 1 ( ICAM 1 ) functions as a ligand of leukocyte function associated antigen 1 ( LFA 1 ) , as well as a receptor for human picorna virus , and its regulation thus affects various immunologic and inflammatory reactions . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Interactions of the ligand / receptor pair LFA 1 ( CD11a / CD18 ) and ICAM 1 ( CD 54 ) initiate and control the cell cell interactions of leukocytes and interactions of leukocytes with parenchymal cells in all phases of the immune response . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Adhesion molecules in the prognosis of diffuse large cell lymphoma : expression of a lymphocyte homing receptor ( CD 44 ) , LFA 1 ( CD11a / 18 ) , and ICAM 1 ( CD 54 ) . ^^^ To investigate whether lymphocyte adhesion molecules involved in the migration of normal lymphocytes , i . e . , CD 44 homing receptor , LFA 1 ( CD11a / 18 ) , and ICAM 1 ( CD 54 ) , also play a role in the spread and hence in the disease course of non Hodgkin ' s lymphomas ( NHL ) , expression of these molecules was examined in 78 cases of diffuse large cell lymphoma . ^^^ Neither LFA 1 nor ICAM 1 expression showed a significant correlation to lymphoma dissemination or disease course . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Phorbol esters induce differentiation of U 937 human promonocytic cells in the absence of LFA 1 / ICAM 1 mediated intercellular adhesion . ^^^ Intercellular adhesions which occur during the mononuclear phagocyte differentiation are predominantly mediated by the lymphocyte function associated antigen 1 ( LFA 1 ) family and the intercellular adhesion molecule 1 ( ICAM 1 ) which is a ligand for LFA 1 . ^^^ Thus , differentiation of U 937 promonocytic cells induced by phorbol esters occurs concomitantly with intercellular LFA 1 / ICAM 1 dependent cluster formation . ^^^ Since these homotypic adhesions can be inhibited by monoclonal antibodies ( mAb ) directed to either LFA 1 or ICAM 1 , we have analyzed whether the lack of cell cell adhesions impairs the differentiation process . ^^^ Treatment of U 937 cells with the phorbol ester 12 O tetradecanoylphorbol 13 acetate in the presence of mAb to LFA 1 or ICAM 1 antigens yielded cells free from homotypic adhesions but differentiated as evidenced by their decreased proliferation and enhanced capacity for generation of superoxide anion . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In addition , latent membrane protein , B lymphocyte activation antigen ( CD 23 ) , and cell adhesion molecules ( ICAM 1 , CD 18 ) all were expressed in the EBV positive infiltrating lymphoproliferative lesions in each mouse . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Formation of MGC could almost entirely be inhibited by mAb to the alpha or beta chain of LFA 1 and to a lesser extent by relatively high concentrations of a mAb against ICAM 1 , one of the ligands of LFA 1 . ^^^ IFN gamma , but not the concanavalin A induced supernatant clearly enhanced expression of LFA 1 and ICAM 1 on monocytes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In addition , adhesion of neonatal and adult neutrophils to purified ICAM 1 in artificial planar membranes was comparable and almost completely inhibited by anti LFA 1 MoAb . ^^^ Chemotactic stimulation induced Mac 1 dependent adhesion of adult neutrophils to endothelial cells , purified intercellular adherence molecule 1 ( ICAM 1 ) and protein coated glass . ^^^ These findings indicate that diminished transendothelial migration by neonatal neutrophils is related to abnormal interactions of Mac 1 with ICAM 1 and possibly other endothelial ligands . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Both types of endothelial cells possess several kinds of Cell Adhesion Molecules ( CAMs ) , such as ICAM 1 , CD 44 , LFA 3 , but not LFA 1 or CD 2 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
ICAM 1 is a cell surface glycoprotein which is one of the ligands for the leukocyte function associated antigen ( LFA 1 ) . ^^^ The quantitative expression of ICAM 1 in various cell types can be either induced or enhanced by treatment with cytokines , such as interferon gamma ( IFN gamma ) , tumor necrosis factor ( TNF ) alpha or interleukin 1 ( IL 1 ) , a phenomenon which results in the augmentation of binding to LFA 1 positive cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The expression pattern of well defined cell surface adhesion receptors called VLA family , LFA 1 and ICAM 1 was determined semiquantitatively in biopsies of oral ( n = 12 ) and dermal lichen planus ( n = 5 ) and compared to normal uninvolved human oral mucosa ( n = 12 ) and skin ( n = 12 ) using an indirect immunoperoxidase technique . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
ICAM 1 ( CD 54 ) is expressed on endothelial cells and serves as an important ligand for the white cell adhesion molecule CD11a / CD18 ( LFA 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In normal monocytes , CD 18 and CD11c are concentrated at specific adhesion sites , named podosomes , together with actin , vinculin , and talin , while CD11a , CD11b , CD29 / beta 1 , CDw49d / alpha 4 and CD54 / ICAM 1 retain a diffuse distribution on the cell surface without a selective pattern of localization . ^^^ Further , in these cells CD 18 , CD11a , CD11c , ICAM 1 , and talin , but not vinculin , co localize in homotypic cell junctions , thus showing a close relationship between integrins and talin . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Distribution of intercellular adhesion molecule 1 ( ICAM 1 ) and lymphocyte function associated antigen 1 ( LFA 1 ) in epidermal tumours . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
It is possible that the cytotoxic potential is regulated by contacts between the members of the lymphocyte population and that these events occur via their ICAM 1 and LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Furthermore , the number of ICAM 1 positive cells in RCC was significantly correlated with the degree of mononuclear cell infiltration , involving mainly T lymphocytes and a smaller number of macrophages bearing the LFA 1 molecule . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
A new monoclonal antibody ( MoAb ) HA 58 ( IgG 1 ) was prepared , which recognizes the binding site on the intercellular adhesion molecule 1 ( ICAM 1 ) antigen to the lymphocyte function associated antigen 1 ( LFA 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Human thymic epithelial cells grown in vitro bind to mature and immature thymocytes via CD 2 and CD11a / CD18 ( LFA 1 ) molecules on thymocytes and by CD 58 ( LFA 3 ) and CD 54 ( ICAM 1 ) molecules on thymic epithelial cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The marked predominance of IgG 3 in HCL suggests a deviation in heavy chain class switching that may be related to the characteristically very low expression of LFA 1 and ICAM 1 molecules on hairy cells , and hence a defect in T cell hairy cell interaction . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
LFA 1 , Mac 1 , and CD 45 ) but expressed ICAM 1 , NCAM , J11d , CD 44 , and MHC molecules . ^^^ LFA 1 , Mac 1 , and CD 45 ) but expressed ICAM 1 , NCAM , J11d , CD 44 , and MHC molecules . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
ICAM 1 and LFA 1 expressions in the lesional skin of annular erythema associated with Sjgren syndrome . ^^^ ICAM 1 and LFA 1 expression was studied in the lesional skin of ten cases of annular erythema associated with Sjgren syndrome . ^^^ ICAM 1 expression on vascular endothelial cells was similar to LFA 1 , although relatively focal and weak expression was observed on mononuclear cells . ^^^ These findings suggest that strong expression of ICAM 1 on endothelial cells but not keratinocytes and LFA 1 on mononuclear cells might play some role in the induction of skin lesions in annular erythema associated with Sjgren syndrome . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The binding of leukocyte function associated antigen 1 ( LFA 1 ) ( CD11a / CD18 ) to its natural target ligand , intercellular adhesion molecule 1 ( ICAM 1 ) ( CD 54 ) , is an important step in lymphocyte adhesion to cells and its subsequent activation . ^^^ We studied whether LFA 1 ICAM 1 interactions affect tumor cell susceptibility to MHC unrestricted lysis by TCR CD 3 CD16+ natural killer ( NK ) and TCR gamma delta + CD3+ CD16+ / lymphocytes . ^^^ Our data demonstrate that LFA 1 ICAM 1 interactions increase MHC unrestricted or CD 16 mediated cytolysis of tumor cells . ^^^ Anti CD 18 ( LFA 1 beta ) mAb inhibited MHC unrestricted lysis of ICAM 1+ and ICAM 1 tumor cells . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Involvement of lymphocyte function associated antigen 1 ( LFA 1 ) but not ICAM 1 in a radioactive leukocyte cell mediated immunity ( LA CMI ) assay . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Synovial lining cells express numerous macrophage associated antigens , including CD11b ( CR 3 ) , CD 13 , CD 14 , CD 16 ( FcRIII ) , CD 18 , CD 32 ( FcRII ) , CD 45 ( leucocyte common antigen ) , CD 54 ( ICAM 1 ) , CD 64 ( FcRI ) , CD 68 , and CD 71 ( transferrin receptor ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The lymphocyte function associated molecule 1 ( LFA 1 , CD11a / CD18 ) is an integrin that mediates adhesion of immune cells by interaction with two members of the Ig superfamily , ICAM 1 and ICAM 2 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Two main molecular adhesion pathways have been characterized involving the CD 2 molecule binding the LFA 3 molecule and the LFA 1 molecule binding the ICAM 1 or ICAM 2 molecule . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
One key element is the in vivo movement of T cells that express LFA 1 into the epidermis , and their subsequent binding to keratinocytes via the surface expression of intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Four distinct receptor / ligand interactions have been elucidated : LFA 1 / ICAM 1 , VLA 5 / fibronection , VLA 4 / fibronectin , and VLA 6 / laminin . ^^^ The existence of multiple adhesion pathways , of multiple ligands for a single receptor ( such as LFA 1 / ICAM 1 and LFA 1 / ICAM 2 ) , multiple receptors for a single ligand ( such as VLA 4 / FN and VLA 5 / FN ) , and regulation of ligand expression ( ICAM 1 ) provides opportunities for co operativity , rebundancy and diversity which the T cell utilizes to exquisitely regulate its adhesive interactions . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In contrast , involvement of the ICAM 1 molecule ( ligand of receptor LFA 1 ) in pathology seems to be considerable . ^^^ ICAM 1 is expressed by keratinocytes in many benign and malignant skin diseases and this makes possible interaction with LFA 1 lymphocytes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
LMP 1 expression in all cell lines induced growth in large clumps and expression of the cellular adhesion molecules ICAM 1 , LFA 1 , and LFA 3 in those cell lines which constitutively express low levels . ^^^ LMP 1 expression induced marked homotypic adhesion in the BJAB cell line , despite the fact that there was no significant increase in the high constitutive BJAB LFA 1 and ICAM 1 levels , suggesting that LMP 1 also induces an associated functional change in these molecules . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Other PMN surface markers ( My 4 , My 7 , ICAM 1 , LFA 1 , LFA 3 , Mac 1 ) did not change significantly . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
LMP influenced in a dose dependent manner the expression of the adhesion molecules LFA 1 , LFA 3 and ICAM 1 and B cell activation markers CD 23 and CD 39 in transfected sublines of BL 41 , but did not affect the expression of these markers in the DG 75 and BJAB cell line . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To determine the possible contribution of adhesion receptors to these restrictions , we have studied binding between the murine and human homologues of LFA 1 ( CD11a / CD18 ) and ICAM employing purified human LFA 1 and ICAM 1 ( CD 54 ) bound to solid substrates . ^^^ Murine cell lines bind to purified human LFA 1 through ICAM 1 and at least one other counter receptor . ^^^ In contrast to binding of murine ICAM 1 to human LFA 1 , murine LFA 1 does not bind to human ICAM 1 . ^^^ The species specificity maps to the LFA 1 alpha subunit , because mouse 10 human hybrid cells expressing the human alpha subunit associated with a mouse beta subunit bind to human ICAM 1 , whereas those with a human beta subunit associated with a murine alpha subunit do not . ^^^ Increased adhesiveness for ICAM 1 stimulated by phorbol esters could be demonstrated for hybrid LFA 1 molecules with human alpha and murine beta subunits . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The lymphocyte adhesion ' receptor ' LFA 1 , a member of the integrin family , interacts with ICAM 1 and other counter receptors to mediate adhesion . ^^^ The LFA 1 / ICAM 1 interaction is regulated by signals transmitted from the cytoplasm to the extracellular space . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We do not find a role for CD 28 , LFA 1 : ICAM 1 , VLA 4 : VCAM 1 or CD 44 in this system . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Monoclonal anti Leu 13 triggered homotypic aggregation of leukemic B cells via an adhesion pathway that was not inhibited by antibodies to leukocyte function associated antigen 1 ( LFA 1 ) or intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Studies using an alternate assay of adhesion ( conjugate formation ) confirm the roles of CD2 / LFA 3 and also implicate a second mode of binding via LFA 1 on T cells to ICAM 1 on L 428 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
It does not appear to involve known cell adhesion molecules ( CAMs ) for which monoclonal antibodies are available ( integrins , N CAM , LFA 1 , and ICAM 1 ) , and we were unable to demonstrate a role for the progenitor cell antigen CD 34 in progenitor cell adhesion to cultured stroma . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Pretreatment of lymphocytes with antibodies against CD 11a and CD 18 ( LFA 1 alpha and beta chain , respectively ) could decrease the lymphocyte binding , whereas ICAM 1 pretreatment of tissue sections was ineffective . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
They have a weak to moderate expression of RFD 1 , C3biR , Fc gamma R , p 150 / 95 , MHC class 1 molecules HLA ABC , and of the adhesion molecules LFA 3 and ICAM 1 , whereas no expression of LFA 1 and several monocyte / macrophage markers were detected . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The results showed that , unlike BAL and peripheral blood CD4+ cells of healthy subjects , almost all BAL CD4+ cells of the patients highly express , besides CDw 29 antigen , LFA 1 and ICAM 1 adhesion molecules . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Efficient presentation of antigen to T cells has also been shown to depend on accessory molecules of adhesion such as intercellular adhesion molecule 1 ( ICAM 1 ) and leukocyte function associated antigen 1 ( LFA 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Of 15 cases tested for the expression of CD11a and CD 18 lymphocyte function associated ( LFA 1 ) antigens and their ligand ICAM 1 ( CD 54 ) , seven were positive and six negative for the three markers , while the other two cases expressed alternatively the two molecules . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Pretreatment of lymphocytes with mAb to the accessory molecules CD 2 , CD 3 , CD 4 , CD 8 , CD11b , or CD11c did not interfere with their response to HD cells . mAb to CD11a ( LFA 1 ) , the alleged ligand of ICAM 1 , inhibited the ICAM 1 but not the HD lectin mediated lymphocyte stimulation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Cryostat sections were stained by the peroxidase antiperoxidase technique employing murine monoclonal antibodies to CD 11 , CD 18 , and ICAM 1 . ^^^ The effect of in vivo interferon gamma on the distribution of LFA 1 and ICAM 1 in normal human skin . ^^^ Lymphocyte function associated antigen 1 ( LFA 1 ) and its ligand intercellular adhesion molecule 1 ( ICAM 1 ) are cell surface adhesion molecules important in many lymphocyte mediated responses . ^^^ In view of the importance of keratinocyte / lymphocyte interactions in the pathogenesis of cutaneous disease , we have examined the effects of in vivo IFN gamma on cutaneous expression of LFA 1 and ICAM 1 . ^^^ Furthermore , after administration of 10 micrograms of IFN gamma LFA 1 positive ( LFA + ve ) lymphocytes were observed along the basement membrane zone closely related to ICAM 1 + ve basal keratinocytes and also surrounding dermal endothelium . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The binding is elicited by both CD2 / LFA 3 and LFA 1 / ICAM 1 interactions . ^^^ Immediately after separation , thymic epithelial cells ( TEC ) readily expressed ICAM 1 , which is one of the ligands of LFA 1 cell adhesion molecules . ^^^ The increase in binding seemed to be caused by the LFA 1 / ICAM 1 interaction , since it was inhibited by anti ICAM 1 monoclonal antibody ( mAb ) and anti LFA 1 mAb . ^^^ This suggests that the LFA 1 / ICAM 1 interaction is also involved in vivo with the binding of thymocytes to TEC , which have been shown to express ICAM 1 . ^^^ These findings indicate that CD3dull+ cells can bind to TEC via CD2 / LFA 3 and LFA 1 / ICAM 1 interactions . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Leucocyte interaction with other cells is facilitated by the adhesion molecules , leucocyte function associated antigen 1 ( LFA 1 ) binding to intercellular adhesion molecule 1 ( ICAM 1 ) and for T cells and natural killer ( NK ) cells the binding of LFA 2 ( CD 2 ) to LFA 3 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In this review Malegapuru Makgoba , Martin Sanders and Stephen Shaw focus primarily on the two molecular pathways of lymphocyte adhesion that have been shown to play a critical role in facilitation of antigen specific recognition , namely CD 2 and its ligand , lymphocyte function associated antigen 3 ( LFA 3 ) , and LFA 1 and its ligand , intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies against LFA 1 or its ligand ICAM 1 accelerate CD 2 ( T11 . 1 + T11 . 2 ) mediated T cell proliferation . ^^^ Monoclonal antibodies ( mAbs ) reactive with either the alpha and beta chains of the lymphocyte function associated antigen 1 ( LFA 1 ) molecule or one of its ligands , intercellular adhesion molecule 1 ( ICAM 1 ) , were found to accelerate anti CD 2 induced proliferation . ^^^ This effect was seen on thymocytes and resting or preactivated T cells ( phytohemagglutinin blasts and alloproliferative T cell clones ) and could be observed , following the introduction of anti LFA 1 or ICAM 1 mAbs , up to 50 hr after the CD 2 stimulatory signal . ^^^ The effects of anti LFA 1 or anti ICAM 1 on T cell activation through the CD 2 pathway were therefore opposite to those observed in the CD 3 pathway , where both mAbs strongly delayed T cell proliferation . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In humans , lymphocyte adhesion to cells is mediated by the protein heterodimer CD11a / CD18 ( Leu CAMa , LFA 1 ) and its ligand CD 54 ( ICAM 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule 1 ( ICAM 1 ) , putatively expressed by antigen presenting or target skin cells , is a ligand for the lymphocyte function associated antigen ( LFA 1 ) present on circulating lymphocytes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The expression of intercellular adhesion molecule 1 ( ICAM 1 ) , a ligand for the leucocyte adhesion receptor lymphocyte function associated antigen 1 ( LFA 1 ) , was studied on liver tissue after transplantation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Furthermore , purified MALA 2 crosslinked to a solid support binds Con A blasts that express LFA 1 , the putative receptor for ICAM 1 , and the binding can be blocked by YN1 / 1 antibody or antimurine LFA 1 antibody indicating a direct interaction of these molecules in cell adhesion . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
All leucocyte subtypes showed specific binding to the ICAM 1 transfectants , their adhesion being inhibited by Fab 1 fragments of W CAM 1 antibody and LFA 1 MoAb ( leucocyte function antigen ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
CD11a , CD 18 , and ICAM 1 were demonstrated on up to 80 % of HIV 1 infected H 9 T cells by flow cytometry ; these molecules were not evident on uninfected H 9 . ^^^ Cell surface expression of CD11a and CD 18 , but not ICAM 1 , was increased on HIV infected , as compared to uninfected U 937 and THP 1 monocytoid cells . ^^^ Preincubating the monocytoid cells with anti CD 18 or anti CD11a or preincubating endothelial cells with anti ICAM 1 suppressed these cell to cell interactions . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Structure and regulation of the leukocyte adhesion receptor LFA 1 and its counterreceptors , ICAM 1 and ICAM 2 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
It is thus likely that 33C4 is a unique molecule involved in B cell aggregation and is clearly distinct from other cell adhesion molecules such as LFA 1 , ICAM 1 , and LFA 3 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We next examined the role of ICAM 1 on EC as a ligand for CD 18 . ^^^ To determine if CD11b CD 18 has the ability to recognize ICAM 1 , human macrophages were plated on culture surfaces coated with purified ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
First , BL cell lines which stably retain the original tumour cell phenotype on serial passage in vitro show very low expression of two cell adhesion related molecules , LFA 1 and ICAM 1 , and are negative for a third such molecule , LFA 3 ; these molecules are thought to play a crucial role in the non antigen dependent phase of effector : target cell conjugation which precedes antigen specific recognition and target cell lysis . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
LFA 1 is a member of the integrin family of adhesion receptors and one of its ligands is intercellular adhesion molecule 1 ( ICAM 1 ) ; a ligand for CD 2 is LFA 3 ; and ligands for CD 4 and CD 8 appear to be major histocompatibility complex class 2 and class 1 molecules , respectively . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
These LMP expressing cells exhibit increased homotypic adhesion due to increased expression of the adhesion molecules LFA 1 and ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
LFA 1 , LFA 3 , and ICAM 1 expression in Burkitt ' s lymphoma . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Functional evidence that intercellular adhesion molecule 1 ( ICAM 1 ) is a ligand for LFA 1 dependent adhesion in T cell mediated cytotoxicity . ^^^ Although intercellular adhesion molecule 1 ( ICAM 1 ) has been implicated as a ligand in some LFA 1 dependent adhesion , its importance to T cell function has not been established . ^^^ Analysis of monoclonal antibody ( mAb ) inhibition of AIC formation indicate that ICAM 1 mAb 1 blocks ( a ) AIC formation with some but not all targets ; ( b ) the LFA 1 pathway but not the CD2 / LFA 3 pathway of adhesion ; ( c ) by binding to the target cell , not the T cell . ^^^ The data also indicate that ICAM 1 is not used in LFA 1 dependent CTL interactions with all kinds of target cells , suggesting the existence of alternative ligands for LFA 1 . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
A putative ligand for LFA 1 is the intercellular adhesion molecule ICAM 1 ( refs 3 5 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Conjugate formation of activated LFA 1 T lymphocytes was mostly mediated by the CD 2 LFA 3 adhesion pathway while the ICAM 1 molecule , a ligand of LFA 1 , had no function . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The cell surface expression and function of the LFA 1 ligand , intercellular adhesion molecule 1 ( ICAM 1 ) , on epidermal keratinocytes ( EK ) was studied . ^^^ All of the adhesion to EK was inhibited by LFA 1 and ICAM 1 mAbs , but not by HLA DR , CD 2 , or LFA 3 mAbs . ^^^ ICAM 1 purified from the HeLa epithelioid cell line and reconstituted into planar membranes also supported efficient adhesion of T lymphoblasts that was blocked by LFA 1 mAb bound to the T lymphoblasts or ICAM 1 mAb bound to the planar membranes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Here we show that the cell surface adhesion molecules LFA 1 , ICAM 1 , and LFA 3 are expressed at very low levels , if at all , on group I / II lines and are coordinately upregulated as BL lines move towards group 3 . ^^^ The change to growth in aggregates reflects the increasing availability of LFA 1 and ICAM 1 , the two ligands whose mutual interaction underlies homotypic BL cell adhesion in vitro . ^^^ The low levels of ICAM 1 and LFA 3 on group I / II BL cell lines are also associated with an impaired ability to interact with EBV specific CTL in the antigen independent phase of effector / target conjugation . mAb blocking studies show that the small number of conjugates that are formed with group I / II BL targets involve the LFA 1 / ICAM 1 adhesion pathway but not the LFA 3 pathway ; in contrast , both pathways contribute to the efficient conjugate formation shown by group 3 BL or LCL targets . ^^^ Here we show that this line has an anomalous pattern of adhesion molecule expression with high levels of LFA 1 and ICAM 1 in the absence of detectable LFA 3 . ^^^ The WW 1 BL cells form conjugates with EBV specific CTL through the LFA 1 / ICAM 1 pathway , but in the absence of a target LFA 3 / effector CD 2 interaction these conjugates do not achieve target cell lysis . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Human neutrophil ( PMN ) attachment to human umbilical vein endothelial cells ( HUVEC ) was evaluated in vitro using two MAbs , R 6 5 D 6 and RR1 / 1 , that recognize intercellular adhesion molecule 1 ( ICAM 1 ) , and one MAb , TS1 / 18 , that recognizes CD 18 . ^^^ These results demonstrate that attachment and transendothelial migration of PMN in vitro depend substantially on both CD 18 on the PMN and ICAM 1 on the endothelial cell . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Lymphocyte function associated antigen 1 ( LFA 1 ) interaction with intercellular adhesion molecule 1 ( ICAM 1 ) is one of at least three mechanisms for lymphocyte adhesion to cultured endothelial cells . ^^^ Adhesion to endothelial cell ICAM 1 in planar membranes was blocked completely by monoclonal antibodies to lymphocyte function associated antigen 1 ( LFA 1 ) or ICAM 1 . ^^^ The LFA 1 dependent adhesion could be further subdivided into an LFA 1 / ICAM 1 dependent component which was increased by cytokines and a basal LFA 1 dependent , ICAM 1 independent component which did not appear to be affected by cytokines . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibody RR 1 / 1 directed against the putative LFA 1 ligand molecule intracellular adhesion molecule 1 ( ICAM 1 ) was found to inhibit the T cell proliferative response to the antigen PPD . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
ICAM 1 a ligand for LFA 1 dependent adhesion of B , T and myeloid cells . ^^^ This constitutes biochemical evidence that ICAM 1 functions as a ligand for LFA 1 dependent adhesion by a variety of leukocytes . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Purified intercellular adhesion molecule 1 ( ICAM 1 ) is a ligand for lymphocyte function associated antigen 1 ( LFA 1 ) . ^^^ Its broad distribution , regulated expression in inflammation , and involvement in LFA 1 dependent cell cell adhesion have suggested that ICAM 1 may be a ligand for LFA 1 . ^^^ LFA 1+ but not LFA 1 cells bound to ICAM 1 in the artificial membranes , and the binding could be specifically inhibited by anti ICAM 1 treatment of the membranes or by anti LFA 1 treatment of the cells . ^^^ The cell binding to ICAM 1 required metabolic energy production , an intact cytoskeleton , and the presence of Mg2+ and was temperature dependent , characteristics of LFA 1 and ICAM 1 dependent cell cell adhesion . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Recently , ICAM 1 ( intercellular adhesion molecule 1 ) has been defined as a ligand for LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
ICAM 1 is a ligand of lymphocyte function associated antigen 1 ( LFA 1 ) , an alpha beta complex that is a member of the integrin family of cell cell and cell matrix receptors . ^^^ The ICAM 1 and LFA 1 interaction is heterophilic and unusual in that it is between members of the immunoglobulin and intergrin families . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
A human intercellular adhesion molecule ( ICAM 1 ) distinct from LFA 1 . ^^^ ICAM 1 is distinct from LFA 1 in both cell distribution and structure . ^^^ However , aggregation of one T lymphocyte cell line ( SKW 3 ) was inhibited by LFA 1 but not ICAM 1 MAb . ^^^ It is proposed that ICAM 1 may be a ligand in many , but not all , LFA 1 dependent adhesion reactions . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The intracellular adhesion molecules ( ICAM 1 ) and lymphocyte function associated antigen 1 ( LFA 1 ) were not found on epithelial glandular salivary cells of patients and controls . ^^^ In conclusion , these data suggested that the absence of ICAM 1 and LFA 1 in salivary cells and the absence of infiltrating T cells bearing gamma delta receptors exclude their immunopathogenetic role in SS ; moreover , these data demonstrated that the aberrant expression of HLA class 2 molecules on epithelial salivary cells of patients with SS is not a phenomenon correlated with the lymphocytic infiltration . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Kidney sections were stained for monocytes and adhesion molecules ( ICAM 1 , VCAM 1 , LFA 1 , and VLA 4 ) using monoclonals , while potentially O 2 ( ) producing neutrophils ( ie , activated neutrophils ) were quantified using immunohistochemical methods . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Antibody inhibition studies demonstrated that adhesion between T cells and B7 . 1 transfected melanoma cells was mediated by interactions between LFA 1 : ICAM 1 and CD 2 : LFA 3 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The adhesive interaction between CD 18 on lymphocytes and ICAM 1 on alveolar epithelial cells was demonstrated by the use of blocking antibodies specific for both ligands . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The adhesion molecules intercellular adhesion molecule 1 ( ICAM 1 ) , lymphocyte function associated antigen 1 ( LFA 1 ) ( alpha and beta chains ) , and very late activation antigen 4 ( VLA 4 ) have an essential role in cell cell interactions and the initiation of immune responses . ^^^ Overall , ICAM 1 and LFA 1 alpha expression was significantly higher in allografts , although there was variable expression of these molecules in isografted animals . ^^^ There were temporal differences in the expression of ICAM 1 and LFA 1 alpha in that increased ICAM 1 expression was more pronounced in the the early posttransplant period , whereas there was a progressive increase in LFA 1 alpha as rejection developed . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Lymphocytes positive for lymphocyte function associated antigen ( LFA 1 ) , a counter receptor of ICAM 1 , were densely distributed along the invasive margin , and sparsely in the stroma within carcinoma . ^^^ Our study suggests that the invasive margin is an area similar to active inflammation , where the antigen presenting cells ( macrophages ) and lymphocytes may interact via the ICAM 1 / LFA 1 adhesion . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Most inflammatory cells were strongly positive for LFA 1 , VLA 4 , Pgp 1 and ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Furthermore , costimulatory signals were provided by UV Mph ; CTLA 4 Ig and LFA 3 Ig fusion proteins and Abs to CD 2 , LFA 3 , LFA 1 , and ICAM 1 inhibited UV Mph induced T cell proliferation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
A series of cutaneous and mucosal lesions were immunolabeled with monoclonal antibodies to the major histocompatibility complex class 1 ( beta 2 microglobulin ) and 2 ( HLA DR antigens ) , intercellular adhesion molecule ( ICAM 1 ) and glycoprotein CD 36 ( OKM 5 ) as well as CD1a ( Langerhans cells ) , CD 4 , CD 8 ( T cells ) and CD11a ( LFA 1 antigen ) . ^^^ This HLA DR expression may be correlated with an up regulation of ICAM 1 and the presence of LFA 1 positive leukocytes , mainly of CD 8 phenotype , in the epithelium . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Previously , we studied the surface expression of LFA 1 ( CD11a / CD18 ) and ICAM 1 ( CD 54 ) in a panel of 16 human T cell lines and found that those carrying HTLV 1 pro viruses expressed high levels of ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
This study was designated to investigate the expression of three adhesion molecules ICAM 1 ( CD 54 ) , VCAM 1 , and ELAM 1 , and the counter ligands LFA 1 ( CD 18 ) , Mac 1 ( CD 11b / CD 18 ) , and VLA 4 ( CD 49d ) in frozen sections of endomyocardial biopsies ( EMB ) of heart allografts in relation to onset of CMV infection recorded as CMV antigenemia . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To substantiate a mechanism by which these cells may move from the vascular compartment to tissue spaces , we also investigated expression of ELAM ( endothelial leukocyte adhesion molecule ) , ICAM 1 ( intercellular adhesion molecule ) , and CD 18 ( leukocyte function antigen ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Pretreatment of CD4+ T cells with NKI L 16 MoAb , which interact with an activation epitope on LFA 1 alpha chain , enhanced adhesion to ICAM 1 but not B 7 or LFA 3 expressing CHO cells . ^^^ The results suggest that resting naive CD4+ T cells utilize preferentially the CD2 / LFA 3 or CD28 / B7 adhesion pathways upon adhesion to APCs , while memory CD4+ T cells utilize the CD2 / LFA 3 , CD28 / B7 and LFA 1 / ICAM 1 adhesion pathways . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We studied the expression of cell adhesion molecules in seven penetrating keratoplasty specimens with graft failure and in a normal eye bank cornea using immunohistochemical staining and monoclonal antibodies against intercellular adhesion molecule 1 ( ICAM 1 , CD 54 ) , lymphocyte function associated antigen 1 ( LFA 1 , CD11a / CD18 ) , vascular cell adhesion molecule 1 ( VCAM 1 ) , E selectin , and major histocompatibility complex ( MHC ) class 2 antigen ( HLA DR ) . ^^^ LFA 1 is a counter receptor for ICAM 1 , and infiltration with leukocytes expressing either the alpha or beta chain of LFA 1 was found in areas of ICAM 1 expression in four of the seven corneas . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies against the adhesion glycoproteins CD 18 , CD11b , intercellular adhesion molecule 1 ( ICAM 1 ) ( at 10 and 30 minutes ) , and L selectin ( at 10 minutes ) , but not P or E selectin , reduced I / R induced leukocyte adherence and emigration as well as albumin leakage . ^^^ Platelet leukocyte aggregates were formed in postischemic venules ; the number of aggregates was reduced by antibodies against P selectin , CD11b , CD 18 , and ICAM 1 , but not E or L selectin . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
This study investigates the expression of T cell receptor 5 delta 1 chain , interleukin 2 receptor alpha chain ( CD 25 ) and adhesion molecules ICAM 1 ( CD 54 ) , LFA 1 ( CD11a / 18 ) and CD 44 on gamma delta+ T cells by three color flow cytometry on cerebrospinal fluid ( CSF ) and blood cells in patients with multiple sclerosis ( MS ) , other inflammatory neurological diseases ( OIND ) and other neurological diseases ( OND ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
FACS analysis revealed that after systemic infection with lymphocytic choriomeningitis virus a number of cell adhesion molecules , including VLA 4 , LFA 1 , and ICAM 1 , are up regulated on CD8+ cells , whereas the lymph node homing receptor MEL 14 is down regulated during the infection ; only marginal changes were observed for CD4+ cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Furthermore , treatment of the BBECs with antibodies against CD11a ( LFA 1 ) , CD11b ( MAC 1 ) , or ICAM 1 significantly decreased SP induced adherence ( p < 0 . 01 all comparisons ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
This adherence was significantly inhibited by anti ICAM 1 ( P = 0 . 03 ) and anti LFA 1 ( P = 0 . 02 ) , but not by anti VCAM 1 ( P = 0 . 13 ) or by antibody to von Willebrand factor ( P = NS ) . ^^^ The interaction between ICAM 1 on HGEC and LFA 1 on mononuclear cells may be important in the pathogenesis of extracapillary inflammation in glomerulonephritis . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies ( MAbs ) against various adhesion molecules ( LFA 1 , LFA 3 , ICAM 1 , VNR , VLA beta 1 chain ) were unable to block TNF production in co culture of monocytes with a human pancreatic carcinoma ( HPC ) cell line . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Specific amino acids , important for binding VLA 4 include aspartate 40 ( D 40 ) , which corresponds to the acidic ICAM 1 residue glutamate 34 ( E 34 ) previously reported to be essential for binding of ICAM 1 to its integrin counter receptor LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The binding was blocked completely with antibodies to CD 18 and ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The addition of an excess of soluble ICAM 1 to compete for the anti ICAM 1 mAb inhibits the oxidative burst in response to ICAM 1 cross linking but not to CD 18 cross linking . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Using this model , we investigated the release of proinflammatory cytokines ( interleukin IL 1 beta , IL 6 , IL 8 and tumour necrosis factor alpha TNF alpha ) and the expression of cell adhesion molecules ( ELAM 1 , ICAM 1 and LFA 1 ) in two studies involving biopsies as well as lavage and brush techniques . ^^^ These findings correspond to a higher expression of the adhesion receptors ELAM 1 , ICAM 1 and LFA 1 in allergic mucosa . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Freshly isolated LC did not display ICAM 1 ( CD 54 ) , ICAM 2 , LFA 1 ( CD11a ) , and LFA 3 ( CD 58 ) , as detected by double color FACS analysis , using HLA DR expression for LC identification . ^^^ ICAM 2 and LFA 1 were undetectable on cultured LC and attempts to induce this expression by different culture conditions remained unsuccessful . mAb against ICAM 1 , LFA 1 , LFA 3 , and CD 2 , continuously present during culture , inhibited the T cell proliferative response to Candida albicans presented by cultured LC . ^^^ Pretreatment of LC and / or T cells with mAb indicated that anti ICAM 1 and anti LFA 3 inhibited at the LC level , whereas anti LFA 1 and anti CD 2 inhibited at the T cell level . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Specific down regulation of proliferative T cell alloresponsiveness by interference with CD2 / LFA 3 and LFA 1 / ICAM 1 in vitro . ^^^ We found that the presence of a mixture of mAbs specific for CD 2 , LFA 3 , LFA 1 alpha and beta chain , and ICAM 1 during primary MLC leads to down regulation of secondary proliferative responses to Ag from the donor used for priming . ^^^ Interference by mAb with either CD2 / LFA 3 , LFA 1 / ICAM 1 , CD2 / LFA 1 , or LFA 3 / ICAM 1 had a substantial , though less pronounced effect on secondary responsiveness . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Adhesion through the LFA 1 ( CD11a / CD18 ) ICAM 1 ( CD 54 ) and the VLA 4 ( CD49d ) VCAM 1 ( CD 106 ) pathways prevents apoptosis of germinal center B cells . ^^^ Previously , we have shown that the LFA 1 ( CD11a / CD18 ) ICAM 1 ( CD 54 ) and VLA 4 ( CD49d ) VCAM 1 ( CD 106 ) adhesion pathways are involved in FDC B cell interaction . ^^^ Using FDC and B cells derived from human tonsils , we found that only B cells adherent to FDC remain viable : disruption of FDC B cell clusters with mAb against LFA 1 alpha ( CD11a ) , VLA 4 ( CD49d ) , ICAM 1 ( CD 54 ) , or VCAM 1 ( CD 106 ) results in apoptosis of the B cells . ^^^ Together , our data strongly suggest that adhesion of B cells via the LFA 1 ( CD11a / CD18 ) ICAM 1 ( CD 54 ) pathway and VLA 4 ( CD49d ) VCAM 1 ( CD 106 ) pathway contributes to B cell selection . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To determine the role of vascular cell adhesion molecule 1 ( VCAM 1 ) / very late activation antigen 4 ( VLA 4 ) and intercellular adhesion molecule 1 ( ICAM 1 ) / lymphocyte function associated antigen 1 ( LFA 1 ) interactions in causing antigen induced eosinophil and T cell recruitment into the tissue , we studied the effect of the in vivo blocking of VCAM 1 , ICAM 1 , VLA 4 , and LFA 1 by pretreatment with monoclonal antibodies ( mAb ) to these four adhesion molecules on the eosinophil and T cell infiltration of the trachea induced by antigen inhalation in mice . ^^^ The in vivo blocking of VCAM 1 and VLA 4 , but not of ICAM 1 and LFA 1 , prevented antigen induced eosinophil infiltration into the mouse trachea . ^^^ On the contrary , the in vivo blocking of VCAM 1 and VLA 4 , but not of ICAM 1 and LFA 1 , increased blood eosinophil counts after antigen challenge , but did not affect blood eosinophil counts without antigen challenge in sensitized mice . ^^^ The in vivo blocking of VCAM 1 and VLA 4 inhibited antigen induced CD4+ and CD8+ T cell infiltration into the trachea more potently than that of ICAM 1 and LFA 1 . ^^^ In contrast , regardless of antigen challenge , the in vivo blocking of LFA 1 , but not of ICAM 1 , increased blood lymphocyte counts more than that of VCAM 1 and VLA 4 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Constitutive expression of HLA class 2 , LFA 1 alpha ( CD11a ) and ICAM 1 ( CD 54 ) was lower and varied among different cultures , while LFA 2 ( CD 2 ) was absent . ^^^ IFN gamma alone or in combination with TNF alpha strongly enhanced expression of HLA class 1 , HLA DR , DP , DQ , LFA 1 alpha and ICAM 1 , but did not affect expression of LFA 2 ( CD 2 ) and LFA 3 ( CD 58 ) . ^^^ TNF alpha alone induced only HLA class 1 and ICAM 1 , but not HLA class 2 or LFA 1 alpha . ^^^ It could be blocked by antibodies against HLA DR determinants and against the adhesion molecules LFA 1 alpha and ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The expression of cell adhesion molecules ( LFA 1 , LFA 2 , LFA 3 and ICAM 1 ) in skin lesions of leprosy patients was studied , as well as the in vitro expression of these molecules induced with gamma interferon ( IFN gamma ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Sections of 20 nevocellular nevi , 35 dysplastic nevi , 6 melanomas in situ , and 20 malignant melanomas were investigated with respect to their expression of intercellular adhesion molecule 1 ( ICAM 1 ) , inducible cell adhesion molecule 110 ( INCAM 110 ) / vascular cell adhesion molecule 1 ( VCAM 1 ) , E selectin , lymphocyte function associated antigen 1 ( LFA 1 ) , and the integrins for very late antigen ( VLA ) alpha ( alpha ) 2 and VLA alpha 6 ; for these studies , monoclonal antibodies were used and indirect immunoperoxidase and immunofluorescence staining methods were performed . ^^^ The ligand for ICAM 1 , LFA 1 , was confined to mononuclear cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Most clones express Thy 1 , Pgp 1 , ICAM 1 , HSA and B 220 antigen , but are negative for LFA 1 , CD 2 , Mel 14 , Fc receptor , Mac 1 , CD 4 and CD 8 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
ICAM 1 was expressed on ductal epithelial cells , endothelial cells , and the majority of infiltrating lymphocytes as well as its ligand LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The adhesion molecules examined were the leukocyte function antigen ( LFA 1 ) and its ligand intercellular adhesion molecule 1 ( ICAM 1 ) , the receptor alpha 5 beta 3 for vitronectin , its subunits alpha 5 and beta 3 , and the CD 36 receptor for thrombospondin ( TSP ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The morphological evaluation was in each case combined with immunofluorescence ( to detect immunoglobulins and complement fractions ) and immunohistochemistry with a wide panel of monoclonal antibodies for T cells ( CD 2 , CD 3 , CD 4 , CD 8 , gamma delta ) , B cells ( CD 20 , CD 22 ) , macrophages ( CD 68 , MAC 387 ) NK cells ( leu 7 , CD 16 ) , activation markers ( IL 2 R , Ki 67 , transferrin R ) , MHC antigens ( HLA ABC , HLA DR ) , adhesion molecules ( ICAM 1 , VCAM 1 , ELAM 1 , PADGEM , VLA 4 , LFA 1 alpha / beta ) , and growth factors ( EGF , TGF alpha , EGF R ) . ^^^ Of the patients 44 % had acute rejection accompanied by increased infiltration of T cells and macrophages showing enhanced IL 2 R expression , increased tubular and endothelial staining for MHC class 2 , ICAM 1 , and VCAM 1 , and strong leukocytic expression of VLA 4 and LFA 1 alpha / beta . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
With respect to lung vascular injury , dual requirements for both leukocyte function associated antigen 1 and Mac 1 were found as well as for ICAM 1 and E and L selectin but not for VLA 4 and P selectin . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Effect of nonsteroidal anti inflammatory drugs on LFA 1 and ICAM 1 expression in gastric mucosa . ^^^ This study aimed to develop immunohistochemical staining techniques to investigate the distribution and sequence of expression of both leukocyte [ lymphocyte function associated antigen 1 ( LFA 1 ) ] and endothelial [ intracellular adhesion molecule 1 ( ICAM 1 ) ] adhesion molecules in the mucosa after treatment with nonsteroidal anti inflammatory drugs ( NSAIDs ) . ^^^ In control rats there were 803 + / 72 LFA 1 stained cells / mm2 in the deep mucosa , 134 + / 32 cells / mm2 in the superficial mucosa , and 6 . 4 + / 1 . 2 ICAM 1 stained blood vessels / mm2 in the total mucosa . ^^^ We conclude that the adhesion molecules LFA 1 and ICAM 1 are expressed in the normal gastric mucosa and that the number of ICAM 1 stained blood vessels increase rapidly after NSAID treatment . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To study the distribution of intercellular adhesion molecule receptor ( ICAM R , or ICAM 3 ) , a novel ligand for the leukointegrin lymphocyte function associated antigen 1 ( LFA 1 ) , in normal and rheumatoid synovial membranes and to compare this with the distribution of ICAM 1 , ICAM 2 , vascular cell adhesion molecule 1 ( VCAM 1 ) , and endothelial leukocyte adhesion molecule 1 ( ELAM 1 ) . ^^^ Although ICAM R is a ligand for LFA 1 and shares considerable sequence homology with ICAM 1 and ICAM 2 , it does not appear to be expressed by the endothelium of normal or inflamed synovial vessels . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
These results suggest that binding between ICAM 1 and Mac 1 or LFA 1 , VCAM 1 and VLA 4 , not but ELAM 1 and sLe 10 , is mainly involved in eosinophil infiltration into the airway in allergic reaction such as bronchial asthma . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In addition , we demonstrate that cross linking of LFA 1 with immobilized monoclonal antibody , or engagement of LFA 1 with ICAM 1 expressed on the surface of a CHO cell , provide an insufficient costimulus for T cell proliferation initiated by enterotoxin , immobilized alpha CD 3 or phorbol ester . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
CD 18 ( found on leukocytes and dendrocytes ) and its ligand ICAM 1 ( intercellular adhesion molecule ) were also identified in sections to determine if these antigens are operative in lichen planus . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
This effect was only marginally effected by mAb to LFA 1 and ICAM 1 , suggesting that additional interaction molecules play a role in T cell B cell collaboration . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Involvement of the `` 1 ' ' domain of LFA 1 in selective binding to ligands ICAM 1 and ICAM 3 . ^^^ To analyze the binding requirements of LFA 1 for its two most homologous ligands , ICAM 1 and ICAM 3 , we compared the effects of various LFA 1 activation regimes and a panel of anti LFA 1 mAbs in T cell binding assays to ICAM 1 or ICAM 3 coated on plastic . ^^^ A screen of 29 anti LFA 1 mAbs led to the identification of two mAbs specific for the alpha subunit of LFA 1 which selectively blocked adhesion of T cells to ICAM 3 but not ICAM 1 . ^^^ Blocking was not due to a steric interference between anti LFA 1 mAbs and N linked carbohydrate residues present on ICAM 3 but not ICAM 1 . ^^^ A third 1 domain mAb , MEM 83 , has been previously reported to uniquely activate LFA 1 to bind ICAM 1 ( Landis , R . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Morphological aspects of LFA 1 / ICAM 1 and VLA4 / VCAM 1 adhesion pathways in human lymph nodes . ^^^ Monoclonal antibodies specific for the adhesion molecules participating in lymphocyte homing , lymphocyte function associated antigen 1 ( LFA 1 ) and very late antigen 4 ( VLA 4 ) , and their respective ligands , intercellular adhesion molecule 1 ( ICAM 1 ) and vascular cell adhesion molecule 1 ( VCAM 1 ) , were used to characterize their expression pattern in human lymph nodes by immunohistochemical and immunoelectron microscopic techniques . ^^^ The location of LFA 1 positive lymphocytes and selective expression of ICAM 1 on the luminal plasma membrane of high endothelial venule endothelium suggested that the LFA 1 / ICAM 1 adhesion pathway participates only in the initial step of the lymphocyte migration process . ^^^ This suggests that LFA 1 / ICAM 1 and VLA4 / VCAM 1 adhesion pathways play an important role in the lymphocyte recognition of antigen presenting cells . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The staining pattern of a group of adhesion molecules on the immunoreactive cells in the lining layer of lymphocytic infiltrates of the rheumatoid synovial membrane was studied , using monoclonal antibodies by immunoperoxidase staining method against LFA 1 , VLA 4 , VLA 5 , ELAM 1 , and ICAM 1 . ^^^ In germinal center like areas in lymphoid aggregates , most of the cells stained strongly for ICAM 1 and VLA 4 , suggesting that the proliferation of B lymphocytes may be facilitated by LFA 1 and VLA 4 dependent T and B cell interaction . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Differential expression of ICAM 1 , VCAM 1 and their ligands LFA 1 , Mac 1 , CD 43 , VLA 4 , and MHC class 2 antigens in murine Toxoplasma encephalitis : a light microscopic and ultrastructural immunohistochemical study . ^^^ Microglia also expressed Mac 1 and leukocyte function associated antigen 1 ( LFA 1 ) , which are both ligands of ICAM 1 , as well as CD 45 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Aggregation was blocked by monoclonal antibodies to ICAM 1 ( CL18 / 6 ) and CD11a ( R7 . 1 ) and CD 18 ( R15 . 7 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The genes for huICAM 1 ( a ligand for LFA 1 ) and huLFA 3 ( a ligand for CD 2 ) were transfected singly and in combination into a null mouse melanoma background , and clonal populations of cells that stably express ICAM 1 and / or LFA 3 were derived . ^^^ Expression of the introduced ICAM 1 and / or LFA 3 by transfected cells enhanced their ability to bind LAK T cells ; the LFA 1 / ICAM 1 mediated binding was not further enhanced by activation with phorbol 12 myristate 13 acetate . ^^^ This experimental system , which allows ICAM 1 / LFA 1 and CD2 / LFA 3 interactions to occur on the LAK T cell at a site distal from the anti CD 3 signal , extends our understanding of LAK T cell activation by establishing that both LFA 1 / ICAM 1 and CD2 / LFA 3 can mediate co stimulation via adhesion and signaling events . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Positive staining for ICAM 1 , lymphocyte function associated antigen 1 ( LFA 1 ) , and very late antigen 4 ( VLA 4 ) was found on the infiltrating mononuclear cells . ^^^ These results suggest that the LFA 1 / ICAM 1 and ELAM 1 pathways may be responsible for the migration of mononuclear cells into the thyroid glands of patients with Graves ' disease , and that the VLA 4 / VCAM 1 pathway plays a critical role in the cellular interactions that lead to the formation of B memory cells and the excess production of antibodies . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We have previously described the involvement of ICAM 3 in the regulation of LFA 1 / ICAM 1 dependent cell cell interaction of T lymphoblasts . ^^^ We have found that ICAM 3 is also able to mediate LFA 1 / ICAM 1 independent cell aggregation of the leukemic JM T cell line and the LFA 1 / CD18 deficient HAFSA B cell line . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Adhesion was partially blocked by monoclonal antibodies ( mAb ) specific for lymphocyte function associated antigen 1 ( LFA 1 ) and very late antigen 4 ( VLA 4 ) on T cells and ICAM 1 and VCAM 1 on ASM cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The proliferative response of human T cells to allogeneic IFN gamma treated endothelial cells is mediated via both CD2 / LFA 3 and LFA 1 / ICAM 1 and 2 adhesion pathways . ^^^ A distinct inhibition of T cell proliferation was observed with mAbs directed against LFA 1 , CD 2 , or a combination of anti ICAM 1 and 2 . ^^^ Combinations of mAbs directed against T cell adhesion molecules ( LFA 1 , CD 2 , VLA 4 ) or EC adhesion molecules ( ICAM 1 , and 2 , LFA 3 , VCAM 1 ) were able to block T cell proliferation for 100 and 80 % respectively . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We assessed the expression of the adhesion molecules leukocyte function antigen 1 ( LFA 1 , CD11a ) , intercellular adhesion molecule 1 ( ICAM 1 , CD 54 ) , homing associated cell adhesion molecule ( H CAM , CD 44 ) , and c kit ( stem cell factor receptor ) on the CD34+ progenitor population from the leukapheresis products of 23 patients ( LP CD34+ ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Mesothelial cells were also noted to express ICAM 1 , CD 29 , and CD 44 , but not CD 18 or selectins . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Differential expression of two ICAM 1 epitopes and LFA 1 chains in B cell non Hodgkin ' s lymphomas . ^^^ B cell non Hodgkin ' s lymphomas ( B NHL ) and B cell areas of reactive lymphadenopathies were investigated immunohistochemically for expression of two distinct ICAM 1 epitopes , Me14 / D12 and P 3 58 , and the LFA 1 alpha and beta chains . ^^^ Partial or total loss of expression of one or both epitope ( s ) and / or chain ( s ) was evident in all B NHL in function of increasing Working Formulation ( WF ) malignancy grade , with most defects in the high grade tumors , namely the lowest detectability of the ICAM 1 Me14 / D12 and LFA 1 alpha chain , the lowest co expression of ICAM 1 epitopes and LFA 1 chains , and the most frequent simultaneous loss . ^^^ The ICAM 1 and LFA 1 profiles overlapped within the low and intermediate grades , whereas striking differences between the high grade subtypes were detected . ^^^ It is suggested that expression defects of this type may help differentiate malignant from benign lymphoproliferations , and also be involved in the progression of B NHL , since most are observed in high grade tumors , whose ICAM 1 and LFA 1 profiles indicate that their subtypes are the expression of distinct normal B cell differentiation stages . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The biopsy specimens of U NPC showed variable infiltration by leukocytes , which were positive for the integrins lymphocyte function antigen 1 ( LFA 1 ) and alpha 4 / beta 1 , the corresponding ligands for ICAM 1 and VCAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Early hematopoietic CD34+ cells express CD 18 , CD11a , CD49d , CD49e , CD 44 , ICAM 1 , and ICAM 3 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
B and T lymphocyte subsets enter peripheral lymph nodes and Peyer ' s patches without preference in vivo : no correlation occurs between their localization in different types of high endothelial venules and the expression of CD 44 , VLA 4 , LFA 1 , ICAM 1 , CD 2 or L selectin . ^^^ In addition , the expression of CD 44 , VLA 4 , LFA 1 , ICAM 1 , CD 2 and L selectin on B and T lymphocyte subsets of the blood was determined by flow cytometry . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Thus , APC inhibited the production of TNF alpha and prevented down regulation of membrane CD11b , CD 14 , and CD 18 , but had no effect on up regulation of MHC class 2 , ICAM 1 , or IL 2R , down regulation of MO expression of another glycophosphatidylinositol linked protein , CD 59 , or production of reactive oxygen intermediates . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Keratinocytes at sites of cutaneous inflammation have increased expression of intercellular adhesion molecule 1 ( ICAM 1 ) , a cytokine inducible adhesion molecule which binds the leukocyte integrins LFA 1 and Mac 1 . ^^^ In vitro adhesion assays demonstrated that cultured transgenic keratinocytes were superior to normal keratinocytes as a substrate for the LFA 1 dependent binding of mouse T cells , confirming that the transgene encoded ICAM 1 was expressed in a functional form . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Accordingly , stimulated T cell adhesion was markedly inhibited by anti ICAM 1 or anti beta 2 integrin antibody ( 95 97 % inhibition for epithelial cells and 57 67 % inhibition for endothelial cells ) directed against ICAM 1 interaction with lymphocyte function associated antigen 1 ( LFA 1 ; alpha L beta 2 integrin ) . ^^^ The results suggest that 1 ) peripheral blood T cells without exogenous activation exhibit little LFA 1 or VLA 4 dependent adherence except to endothelial or epithelial cells expressing high levels of ICAM 1 and / or VCAM 1 ; and 2 ) differences in endothelial vs . epithelial cell mechanisms to bind activated and unactivated T cells ( e . g . , dependence on a mixed vs . a single ligand system and distinct cytokine responsiveness of ligand levels ) may help to coordinate T cell traffic to epithelial barriers . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In this report , we investigated the role of intercellular adhesion molecule 3 ( ICAM 3 ) , the third identified ligand for the beta 2 integrin leukocyte function associated antigen 1 ( LFA 1 ) , in the regulation of leukocyte adhesion to ICAM 1 , vascular cell adhesion molecule 1 ( VCAM 1 ) , and the 38 and 80 kD fragments of fibronectin ( FN 40 and FN 80 ) . ^^^ T cell adhesion to ICAM 1 , VCAM 1 , FN 40 , and FN 80 was specifically blocked by anti LFA 1 , anti VLA alpha 4 , and anti VLA alpha 5 mAb , respectively . ^^^ We also studied the localization of ICAM 3 , LFA 1 , and the VLA beta 1 integrin , by immunofluorescence microscopy , on cells interacting with ICAM 1 , VCAM 1 and FN 80 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In unstimulated EC layers , anti leukocyte function associated antigen 1 ( LFA 1 ) plus anti intercellular adhesion molecule 1 ( ICAM 1 ) monoclonal antibodies ( mAbs ) inhibited monocyte adhesion and transmigration significantly by 19 % and 20 % , respectively , whereas anti very late antigen 4 ( VLA 4 ) plus anti vascular cell adhesion molecule 1 ( VCAM 1 ) mAbs did not . ^^^ In IL 1 beta stimulated EC layers , anti LFA 1 plus anti ICAM 1 mAbs inhibited the adhesion and transmigration by 32 % and 30 % , respectively and anti VLA 4 plus anti VCAM 1 mAbs did so by 18 % and 27 % , respectively . ^^^ These results suggest that the monocyte EC interaction in unstimulated ECs is mediated , in part , by the LFA 1 ICAM 1 pathway and in IL 1 beta stimulated ECs , in part , by both LFA 1 ICAM 1 and VLA 4 VCAM 1 pathways . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The expression of cell adhesion molecules ( LECAM 1 , LFA 1 , VLA 4 , ICAM 1 and CD 44 ) of lymph nodes from B cell chronic lymphocytic leukaemia ( B CLL ; mature : 16 cases , immature : 8 ) , lymphocytic lymphoma ( LL ; M : 5 , IM : 2 ) and reactive lymph nodes ( 10 ) were investigated in frozen tissue sections . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Immunolocalization for ICAM 1 , the ligand of Mac 1 and LFA 1 , was detected in the endothelial cells of capillaries and venules , in the mononuclear cells in submucosa , and in the basal layer of the epithelium . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Because adhesion properties of leukocytes are important for the influx and localization of leukocytes in sites of inflammation , we studied , the expression of intercellular adhesion molecule 1 ( ICAM 1 ) , lymphocyte function associated antigen 1 ( LFA 1 ) , vascular cell adhesion molecule 1 ( VCAM 1 ) and CD 11b in 14 kidney biopsies of PSGN patients , arbitrarily divided into early biopsies ( less than 15 days after onset of PSGN ) and late biopsies ( 17 90 days ) . ^^^ Our studies demonstrate increased expression of adhesion molecules ICAM 1 and LFA 1 in the kidney of PSGN patients , and these findings were more pronounced in early biopsies ; adhesion molecules are probably involved in the inflammatory infiltration of this disease . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Induction of ICAM 1 and LFA 3 by Tax 1 of human T cell leukemia virus type 1 and mechanism of down regulation of ICAM 1 or LFA 1 in adult T cell leukemia cell lines . ^^^ The present study was undertaken to determine the role of HTLV 1 TaxI in the up regulation of ICAM 1 and LFA 3 in human T cells transformed with HTLV 1 and the mechanism of down regulation of ICAM 1 and LFA 1 in ATL derived cell lines . ^^^ The mechanism of down regulation of ICAM 1 or LFA 1 in 4 ATL cell lines was next examined . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Other ICAM 3 residues critical to adhesive interactions , such as Q 75 , conserved in ICAM 1 and ICAM 2 , but not VCAM 1 , may confer specificity to LFA 1 binding . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The constitutional expression of the L selectins ( LECAM 1 ) , LFA 1 / CD11a , VLA alpha 4 / CDw49d and ICAM 1 / CD54 adhesion molecules was detected , and changes in their density after IFN alpha stimulation were compared to results obtained by the high endothelial venule ( HEV ) binding assay and a carbohydrate ( phosphonomannan core polysaccharide : PPME and fucoidin ) immobilization test . ^^^ The LECAM 1 and ICAM 1 molecules were expressed on the great majority of CLL cells , while the LFA 1 and VLA 4 alpha chains were expressed by only a small number of cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
LFA 1 is also involved in this interaction via its ligand ICAM 1 . ^^^ These results show that the molecular basis of T lymphocyte binding to rheumatoid synovial lining cells is different from that described for T lymphocyte binding to synovial membrane vascular endothelium which involves the VLA 4 / VCAM 1 and VLA 4 / CS 1 pathways , but not the LFA 1 / ICAM 1 pathway . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The expression of other adhesion molecules , LFA 1 alpha ( CD11a ) , LFA 1 beta ( CD 18 ) , Pgp 1 ( CD 44 ) , CD 45 , MEL 14 ( L selectin ) , and VLA 4 alpha ( CD49d ) , were all increased as a result of anti CD 2 treatment , whereas CD 25 ( IL 2R ) , CD 48 ( CD 2 ligand ) , and ICAM 1 ( CD 54 ) remained unchanged . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
T lymphocytes and neutrophils tether and roll on plastic immobilized PNAd and E selectin at 1 . 8 dyn / cm2 wall shear stress , but fail to interact with immobilized ICAM 1 , a ligand for LFA 1 and Mac 1 , at the same flow rate . ^^^ Incubating T lymphocytes on PNAd and ICAM 1 does not result in adhesion strengthening , suggesting that adhesion through PNAd by L selectin does not stimulate lymphocyte LFA 1 avidity for ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Expression of adhesion molecules ICAM 1 , lymphocyte adhesion molecule 1 ( LFA 1 ) , vascular cell adhesion molecule ( VCAM ) , and E selectin was examined together with HLA DR in 16 liver biopsy specimens showing PSC and 12 specimens showing PBC . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
These findings suggest that ICAM 1 / LFA 1 and also VCAM 1 / VLA 4 linkages between the damaged bile ducts and lymphocytes may facilitate antigen specific reactions such as the presentation of antigens , possibly PDC E 2 , to the periductal lymphocytes in PBC . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
METHODS Cell adhesion molecules E selectin , VCAM 1 , ICAM 1 and the receptors LFA 1 and CR 3 were immunolocalised on cryostat sections of the bone implant interface obtained during revision arthroplasty . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
When complexed with CD 18 , all CD11a chimeras bound ICAM 1 at levels comparable to wild type CD11a / CD18 , indicating that the residues in these chimeras which differ in human and murine 1 domains may not play a critical role in LFA 1 binding to ICAM 1 . ^^^ Identification of amino acids in the CD11a 1 domain important for binding of the leukocyte function associated antigen 1 ( LFA 1 ) to intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^ Leukocyte function associated antigen 1 ( LFA 1 ) is a cell surface adhesion receptor for intercellular adhesion molecule 1 , 2 , and 3 ( ICAM 1 , 2 , 3 ) . ^^^ Using human / murine chimeras of the 1 domain of the LFA 1 alpha subunit ( CD11a ) , we recently identified the epitopes recognized by eight monoclonal antibodies against CD11a that inhibit LFA 1 binding to ICAM 1 . ^^^ In this report , we determined that replacement of the entire human 1 domain with the entire murine 1 domain in CD11a completely abrogated LFA 1 binding to human ICAM 1 without affecting the gross conformation or heterodimer formation of LFA 1 , as assayed by antibody binding . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Tumor cells expressing ICAM 1 were frequently detected in squamous cell carcinoma and condyloma and were correlated with a dense infiltration of HLA DR and LFA 1 positive cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Using these methods , various laboratories have reported mutations for a variety of inflammatory cell adhesion molecules including CD 18 , alpha 5 integrin , ICAM 1 , P selectin , and L selectin ; preliminary reports of other mutations are also available . ^^^ Mutations in CD 18 and ICAM 1 cause impaired inflammatory and immune responses , mutations in P selectin and L selectin cause decreased leukocyte rolling and emigration , and a mutation in alpha 5 integrin causes embryonic lethality . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
T cell induced differentiation of B cells has been shown to be dependent on the CD2 / LFA 3 and LFA 1 / ICAM 1 pathways . ^^^ After stimulation of gingival cells in vitro , the per cent CD 2 positive T cells and LFA 3 and ICAM 1 positive B cells remained relatively stable over the six day culture period , although P . gingivalis and F . nucleatum appeared to induce an increase in the percentage of gingival T cells expressing LFA 1 . ^^^ In contrast to the gingival lymphocytes , stimulation of peripheral blood cells resulted in an increase in the per cent CD 2 positive T cells , LFA 3 and ICAM 1 positive B cells , with a decrease in LFA 1 positive T cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Expression of the adhesion molecules LFA 1 , ICAM 1 , and VCAM 1 was studied in five resected colon carcinomas , one villous adenoma , and normal colon mucosa to determine whether expression of these markers is increased in response to tumor . ^^^ Frozen tissue samples were stained by a labeled avidin biotin technique using primary antibodies to LFA 1 ( CD11a ) , CD 2 , CD 4 , CD 8 , CD 20 , CD 68 , HLA DR , ICAM 1 ( CD 54 ) , and VCAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The 5 phosphatase activity , which we previously observed to be defective in HIV disease , was found to correlate linearly with the expression of both LFA 1 and its ligand , ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In comparison with the predominant naive population of mesenteric lymph node T cells , IC T cells express much higher levels of CD 44 , LFA 1 and ICAM 1 , and lower levels of CD45RB , features commonly associated with memory ( previously activated ) cells . ^^^ Consistent with this , in in vitro adhesion assays , brain IC bound better than MLN cells to the alpha 4 beta 1 integrin ligand VCAM 1 and the LFA 1 ligand ICAM 1 but adhered very poorly to the alpha 4 beta 7 ligand MAdCAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In human mycosis fungoides ( MF ) , interactions between LFA 1 ( CD11a / CD18 ) and ICAM 1 ( CD 54 ) are involved in lymphocyte adhesion to keratinocytes . ^^^ The extent of keratinocyte ICAM 1 expression did not correlate with the degree of lymphocyte epithelial infiltration , nor with lymphocyte LFA 1 expression . ^^^ In conclusion , in canine MF , the lack of statistically significant correlations between epithelial lymphocyte infiltration and keratinocyte ICAM 1 expression , and between keratinocyte ICAM 1 and lymphocyte LFA 1 staining , suggests that the LFA 1 / ICAM 1 pathway is not the major adhesion mechanism between lymphocytes and keratinocytes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Bone marrow HPC express the immunoglobulin gene superfamily members of ICAM 1 ( CD 54 ) , PECAM 1 ( CD 31 ) and LFA 3 ( CD 58 ) , the integrins VLA 4 ( CD49d / CD29 ) , VLA 5 ( CD49e / CD29 ) and LFA 1 ( CD11a / CD18 ) , L Selectin ( CD62L ) , HCAM ( CD 44 ) and CD 36 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The majority of them ( 75 95 % ) expressed the CD11b and CD 18 molecules , and 60 70 % expressed CD 54 ( ICAM 1 ) . ^^^ It was found that LFA 1 / CD18 and ICAM 1 play a significant role in the TMF / thymocyte adhesion . ^^^ In addition , a LFA 1 dependent / ICAM 1 independent adhesion pathway was observed , suggesting that LFA 1 might use another ligand . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Furthermore , the aggregation induced by rapamycin was blocked by antibodies to the alpha ( CD11a ) or beta ( CD 18 ) subunits of the integrin , LFA 1 , or to its ligands , ICAM 1 and ICAM 2 , and did not occur in LFA 1 deficient YAC mutants . ^^^ However , the surface expression of LFA 1 , ICAM 1 , or ICAM 2 was not augmented in cells aggregated by rapamycin . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Recently , the malaria binding site on ICAM 1 ( CD 54 ) has been mapped to a site distinct but overlapping with the LFA 1 ( CD11a / CD18 ) site . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
B cell homotypic adhesion through exon A restricted epitopes of CD 45 involves LFA 1 / ICAM 1 , ICAM 3 interactions , and induces coclustering of CD 45 and LFA 1 . ^^^ CD 45 mediated intercellular adhesion was abrogated upon incubation with anti leukocyte function associated antigen 1 ( anti LFA 1 ) , intercellular cell adhesion molecule 1 ( ICAM 1 ) , and ICAM 3 MoAbs , thus indicating that this phenomenon involved both LFA 1 / ICAM 1 and LFA 1 / ICAM 3 cell adhesion pathways . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The inflammatory cellular infiltrates contained varying amounts of cells bearing the VCAM 1 ligand VLA 4 and the ELAM 1 ligand SLeX as well as large amounts of cells expressing LFA 1 alpha and beta , ligands of ICAM 1 . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We investigated the relative contribution of the ICAM 1 : LFA 1 and B 7 : CD28 / CTLA 4 costimulatory pathways in naive T cell activation , using either anti CD 28 Ab or fibroblast cell lines transfected with 1 Ek , which express either no costimulatory molecules , ICAM 1 alone , B 7 1 alone , or ICAM 1 and B 7 1 together . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We obtained the evidence that coadministration in vivo of mAbs against leukocyte function associated antigen 1 ( LFA 1 ) and intercellular adhesion molecule 1 ( ICAM 1 ) suppressed the progression of experimental allergic encephalomyelitis ( EAE ) in rats . ^^^ However , administration of the mAb to LFA 1 alone or ICAM 1 alone did not show such suppressive effects . ^^^ These findings suggest that LFA 1 and ICAM 1 are critically involved in the development of EAE and that the administration together of mAbs against adhesion molecules including LFA 1 and ICAM 1 might provide a new immunotherapeutic approach for the treatment of multiple sclerosis . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The ability of inactivated viruses , bacteria , protozoa and fungi to modulate the expression of CD 14 , CD49d , CD49f , CD11a ( LFA 1 ) , and CD 54 ( ICAM 1 ) molecules in unprimed bone marrow derived mononuclear phagocytes ( BMM phi ) was investigated by means of flow cytometry . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Irradiated EBV B cells showed reduced expression of ICAM 1 ( 30 % ) , LFA 3 ( 25 % ) and B 7 1 ( 35 % ) , while expression of HLA DR , CD 19 and LFA 1 was not affected . ^^^ UVB irradiation of monocytes did result in reduction in the expression of HLA DR ( 30 % ) , LFA 3 ( 40 % ) , ICAM 1 ( 65 % ) AND B 7 1 and B 7 3 ( 90 % ) , but had no effect on CD 14 , LFA 1 and ICAM 3 expression . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The LFA 1 ligands involved in adhesion of resting CD 4 T cells to DC and alloreactive CD4+ T cells to specific DC differed in part , since ICAM 3 on resting T cells and ICAM 1 on alloreactive T lymphocytes preferentially bound LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
It is therefore of interest to evaluate eosinophil activation via intercellular adhesion molecule 1 ( ICAM 1 ) and the beta 2 integrin family , namely CR 3 ( Mac 1 ) , lymphocyte function associated antigen ( LFA ) 1 alpha and LFA 1 beta , which are ligands for ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The present study examined the role of three adhesion molecules , intercellular adhesion molecule 1 ( ICAM 1 ) , lymphocyte function associated antigen 1 ( LFA 1 ) , and very late antigen 4 ( VLA 4 ) , that participate in cellular recruitment , interaction , and lymphocyte activation during in vitro activation of acutely and chronically infected spleen and liver granuloma lymphocytes . ^^^ Blockade of ICAM 1 , LFA 1 , or VLA 4 by rat monoclonal antibody inhibited spleen and granuloma lymphocyte interleukin 2 ( IL 2 ) and IL 4 production as well as lymphoproliferative responses at similar levels ( 66 to 87 % ) . ^^^ Cell sorting analysis demonstrated that acutely and chronically infected splenic and granuloma lymphocytes expressed similar levels of LFA 1 , ICAM 1 , and VLA 4 and that more ICAM 1 was expressed on infected than on uninfected mouse lymphocytes . ^^^ By exposure of cells to paired monoclonal antibodies at suboptimal doses , it was determined that whereas all three adhesion molecules may participate , only ICAM 1 and LFA 1 showed synergistic interactions in determining lymphocyte responsiveness . ^^^ Thus , ICAM 1 , LFA 1 , and VLA 4 play an important accessory role in inflammatory cytokine production and lymphocyte proliferation , and therefore these adhesion molecules may participate in the initiation and maintenance of the granulomatous inflammation . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Antibodies to CD 18 and CD11b reduced migration by 70 to 79 % , suggesting that CD11b / CD18 ( Mac 1 ) was acting as the neutrophil receptor for ICAM 1 in this process . ^^^ In conclusion , E . coli induced ICAM 1 and Mac 1 dependent transepithelial neutrophil migration . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The Western analysis indicate that it does not cross react with antibodies directed against murine ICAM 1 , LFA 1 alpha , VCAM 1 , HSA , and B 7 suggesting the novelty of the protein . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
These results suggest that hepatic GVHD is induced by donor alpha beta T cells through mechanisms that may involve CD 4 : 1Ad and LFA 1 : ICAM 1 interactions . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We have examined sequentially in vitro lymphocyte binding patterns in a kidney transplant model of acute rejection in rats and their relationship to the expression of two representative adhesion molecules , ICAM 1 and LFA 1 . ^^^ Lymphocyte binding could be inhibited ( approx . 40 % ) by monoclonal antibodies directed against LFA 1 and ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Human renal tubular epithelial and renal carcinoma cells express intercellular adhesion molecule 1 ( ICAM 1 ) , a ligand of the leukocyte function associated antigen 1 ( LFA 1 ) . ^^^ ICAM 1 is crucial for the adhesion of LFA 1 expressing lymphocytes and is involved in antigen presentation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To delineate the adhesion proteins involved in the cell cell interactions , assays were performed in the presence of specific blocking monoclonal antibodies to eosinophil CD 18 , CD11a , or CD11b , and REC ICAM 1 molecules . ^^^ In contrast , antibodies to CD 18 , CD11a , and CD11b significantly decreased ( P < 0 . 01 ) eosinophil adhesion , thus demonstrating pivotal roles for the CD11 / CD18 ( beta 2 ) integrins , but not necessarily for ICAM 1 , in interactions between the REC and eosinophils . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Cellular adhesion molecules , such as ICAM 1 , 2 , and 3 ; LFA 1 ; and HLA class 1 and 2 are incorporated into HIV 1 virions during budding from infected cells . ^^^ In this study we investigated the effects of IFN gamma on the incorporation of ICAM 1 , LFA 1 , and HLA DR into mature HIV 1 progeny from chronically infected cells . ^^^ The results indicate that IFN gamma stimulates the expression of ICAM 1 and of HLA DR on HIV 1 infected cells , whereas LFA 1 expression is unaffected . ^^^ The same modifications were also observed on virus progeny , because specific MAbs to ICAM 1 and HLA DR captured infectious HIV 1 from IFN treated cells with higher efficiency as compared to virus from control cells , whereas virus binding to anti LFA 1 MAb was unchanged . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The beta 2 integrin consists of lymphocyte function antigen 1 ( LFA 1 , CD11a / CD18 ) which binds to the major ligand intracellular adhesion molecules 1 and 2 ( ICAM 1 and ICAM 2 ) , Mac 1 ( CD11b / CD18 , ligand iC3b ) and p 150 , 95 ( CD11c / CD18 , ligand iC3b ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Prevention of adoptive transfer of murine Sjgren ' s syndrome into severe combined immunodeficient ( SCID ) mice by antibodies against intercellular adhesion molecule 1 ( ICAM 1 ) and lymphocyte function associated antigen 1 ( LFA 1 ) . ^^^ We have analysed the role of ICAM 1 and LFA 1 during development of autoimmune sialadenitis in MRL / lpr mice by direct analysis of RNA obtained from the salivary gland tissues , and the therapeutic effects with antibody administration on adoptive transfer system into SCID mice . ^^^ Up regulated expression of ICAM 1 mRNA was observed before the onset of inflammatory lesions in the salivary glands at 1 month and 2 months old , and thereafter LFA 1 mRNA was expressed within the typical inflammatory lesions , resembling human Sjgren ' s syndrome in MRL / lpr mice . ^^^ Immunohistochemically , ICAM 1 was localized exclusively in the endothelial cells of varying sized blood vessels before the onset of disease , and LFA 1 expressing inflammatory cells were found within these lesions . ^^^ When the therapeutic effects in vivo were examined , antibodies to ICAM 1 in combination with anti LFA 1 prevented adoptive transfer of Sjgren ' s syndrome in MRL / lpr mice into SCID mice , while no significant effect was found when treated with either antibody . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Increased cellular infiltration and increased expression of E selectin , ICAM 1 , VCAM 1 , PECAM 1 , VLA 4 , and Mac 1 were found in ST from patients with RA compared to ST from patients with OA . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies ( mAbs ) against various adhesion molecules ( LFA 1 , ICAM 1 , VNR ) were unable to block NO production in coculture of monocytes with carcinoma cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We therefore examined the expression of the more recently identified LFA 1 ligand , ICAM 3 ( CD 50 ) , in comparison to ICAM 1 and ICAM 2 on blood DC and sought a functional role for ICAM 3 in DC mediated T lymphocyte responses . ^^^ However , the anti ICAM 3 mAb CAL 3 . 10 inhibited a DC stimulated MLR to a greater extent than anti ICAM 1 or anti ICAM 2 reagents and appeared to act by blocking the DC ICAM 3 T lymphocyte LFA 1 interaction . ^^^ As ICAM 3 is the predominant LFA 1 ligand on resting blood DC , we postulate that DC may utilize ICAM 3 for initial DC T lymphocyte interactions , and that ICAM 1 , which is up regulated upon DC activation , and / or ICAM 2 , may contribute to DC migration or later phases of the T lymphocyte activation process . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
None of these anti CD 44 mAbs induced homotypic cell aggregations was blocked by antibodies against LFA 1 , ICAM 1 , VLA 4 , or L selectin . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The expression of the lymphocyte function associated antigen 1 ( LFA 1 ) and of its ligand , the intercellular adhesion molecule 1 ( ICAM 1 ) has been studied in psoriatic skin lesions of 10 patients with guttate , nummular , and palmoplantar psoriasis . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibody ( Mab ) to ICAM 1 , but not its Fab ' fragment or MAbs to LFA 1 and PECAM 1 , increases HIV 1 infection of HUVEC by enhancing HIV 1 absorption . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Immunohistological studies showed an abundance of intracellular adhesion molecule 1 ( ICAM 1 ) in the germinal centers and thymic medullary epithelium , and the presence of lymphoid function associated antigen 1 ( LFA 1 ) in thymic lymphocytes . ^^^ ICAM 1 and LFA 1 are ligand of adhesion molecules which interact with each other and play an important role in antigen presentation . ^^^ The presence of both ICAM 1 and LFA 1 in the active thymus in this patient suggests that these adhesion molecules may have a role in ACh R antigen presentation , causing myasthenia . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The Ab induces binding of the LFA 1 expressing cell line , JY , to ICAM 1 ( CD 54 ) and ICAM 3 ( CD 50 ) . ^^^ Stimulation of neutrophils with CBR LFA 1 / 2 induces binding to ICAM 1 through activation of both LFA 1 and Mac 1 . ^^^ CBR LFA 1 / 2 lowers by 10 fold the concentration of Mg2+ required to achieve maximal binding of LFA 1 to ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Among various molecules known to provide costimulatory function , the expression of CD 54 ( ICAM 1 ) and CD11a / CD18 ( LFA 1 ) on MAIDS B cells was increased , whereas that of CD 2 , heat stable Ag ( CD 24 ) , CD 80 ( B 7 1 ) , and CD 86 ( B 7 2 ) was unchanged from normal . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Lysis of autologous tumor cells by the clone was inhibited completely by anti 5 delta 2 mAb and partially by mAb against CD 3 , LFA 1 alpha and ICAM 1 molecules , while it was resistant to anti CD 8 , anti HLA ABC and anti HLA DR mAb . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
OBJECTIVES : Intercellular adhesion molecule 1 ( ICAM 1 ) , a 80 110 kD glycoprotein , has been found to be a ligand for the lymphocyte function associated antigen 1 ( LFA 1 ) molecule and has important roles in inflammatory and immune mediated mechanisms . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Further , both the autologous and allogeneic systems could be mediated through adhesion molecules such as ICAM 1 and LFA 3 on melanoma cells and LFA 1 on T cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Anti CD 18 ( beta chain of LFA 1 ) and anti CD 54 ( ICAM 1 ) monoclonal antibodies , by inhibiting LAK cell binding to targets , suppressed the killing activity of mLT+ LAK cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Purified intercellular adhesion molecule 1 ( ICAM 1 ) , a ligand of the LFA 1 , was used to analyze the contribution of ICAM 1 to the activation of CTL . ^^^ This facilitated response is most likely mediated through LFA 1 , since Abs to this molecule significantly inhibit the response , Interestingly , when ICAM 1 and anti CD 3 are immobilized on separate beads and presented to the CTL , no ICAM 1 enhanced degranulation is observed . ^^^ Taken together , these results suggest that LFA 1 is acting principally as an adhesion molecule , with respect to ICAM 1 , in CTL and that this adhesion is regulated through the TCR complex . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies against CD 54 ( ICAM 1 ) and CD11a ( LFA 1 ) prevent and inhibit endotoxin induced uveitis . ^^^ We studied the effect of monoclonal antibodies ( mAbs ) against CD 54 ( intercellular adhesion molecule 1 ; ICAM 1 ) and CD11a ( lymphocyte function associated antigen 1 ; LFA 1 ) on the prevention and treatment of endotoxin induced uveitis ( EIU ) . ^^^ M . on histologic sections was 469 . 2 + / 51 . 9 for controls , 13 . 8 + / 2 . 6 for rats receiving anti ICAM 1 mAb ( P < 0 . 0001 ) , and 195 . 8 + / 48 . 8 for rats receiving anti LFA 1 mAb ( P = 0 . 0003 ) . ^^^ M . was 273 . 0 + / 30 . 7 for controls , 6 . 4 + / 1 . 7 for rats receiving anti ICAM 1 mAb ( P < 0 . 0001 ) , and 54 . 2 + / 7 . 6 for rats receiving anti LFA 1 mAb ( P < 0 . 0001 ) . ^^^ M . was 1867 . 6 + / 321 . 8 for controls , 21 . 7 + / 5 . 3 for rats receiving anti ICAM 1 mAb ( P < 0 . 0001 ) , and 295 . 1 + / 71 . 2 for rats receiving anti LFA 1 mAb ( P < 0 . 0001 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We show that IFN beta downregulates both basal and IFN gamma induced expression of VLA 4 ( very late activation antigen 4 ) antigen on monocytes , but has no effect on the expression of CD11a , CD11b , CD11c , L selectin , CD 18 , ICAM 1 , beta 1 integrin or CD 44 on monocytes or ICAM 1 on EAhy 926 cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Human intercellular adhesion molecule 1 ( ICAM 1 ) , a specific ligand for the leukocyte function associated antigen 1 and for Mac 1 , plays an important role in immune responses . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The 1 domain of the cell surface Mac 1 ( CD11b / CD18 ) integrin has been shown to be the major recognition site for several adhesion ligands , including iC3b , fibrinogen , factor 10 , and ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Blocking of surface ICAM 1 on hKEC or of lymphocyte function antigen 1 ( LFA 1 ) on the blasts significantly inhibited adhesion . ^^^ The combined data suggest the importance of cytokine regulation of LFA 1 / ICAM 1 adhesion molecule in these cellular interactions . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The CD 18 expression on rabbit polymorphonuclears ( PMNs ) and adhesion rate of rabbit PMNs to ICAM 1 coated magnetic beads were determined , and the correlationship was analysed between CD 18 expression and adhesion rate . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We propose that ATL cells proliferate in sequential events : the homotypic and calcium dependent adhesion through LFA 1 / ICAM 1 , the signal transduction through these adhesion molecules , the production of cytokines , and the proliferation . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
A subpopulation of Mac 1 ( CD11b / CD18 ) molecules mediates neutrophil adhesion to ICAM 1 and fibrinogen . ^^^ We report that a subpopulation ( 10 % ) of the Mac 1 ( CD 1 1b / CD18 ) molecules on activated neutrophils mediates adhesion to ICAM 1 and fibrinogen . ^^^ Because CBRM1 / 5 recognizes a fraction of Mac 1 on activated neutrophils , but still blocks Mac 1 dependent adhesion to fibrinogen and ICAM 1 , we suggest that only a small subset of Mac 1 molecules is competent to mediate adhesion . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
General adhesion molecules ( LFA 1 , LFA 3 , ICAM 1 ) are uniformly up regulated concordant with changes in T cell expression of CD45RA / RO in all tissues examined . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The elevated adhesion was decreased with monocytes were preincubated with staurosporine , an inhibitor of intracellular protein kinase activity and was markedly inhibited by mAb against the common beta 2 subunit ( CD 18 ) of the CD11 / CD18 molecules on monocytes and by mAb against ICAM 1 on 24 h rIL 1 alpha stimulated venous EC . ^^^ It has also been observed that the initial adherence of monocytes to cultured cytokine stimulated EC eventually results in an ICAM 1 and LFA 1 ( CD11a / CD18 ) dependent adherence , which coincides with stretching and lateral migration of the monocytes over the surface of EC . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In this report , we use alpha subunit chimeras of Mac 1 ( CD11b / CD18 ) and p 150 , 95 ( CD11c / CD18 ) , and an extended panel of newly generated and previously characterized mAbs specific to the alpha chain of Mac 1 to map the binding sites for four distinct ligands for Mac 1 : iC3b , fibrinogen , ICAM 1 , and the as yet uncharacterized counter receptor responsible for neutrophil homotypic adhesion . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Ui11 / E3 , a natural killer ( NK ) cell resistant murine target cell line genetically engineered to constitutively express human intercellular adhesion molecule 1 ( ICAM 1 ; CD 54 ) was used in a flow cytometric experimental model to evaluate DTT effects on the NK cell integrin adhesion molecule , leukocyte function antigen 1 ( LFA 1 ; alpha L beta 2 , CD11a / CD18 ) . ^^^ DTT and several structurally related dithiol compounds elicited a dramatic elevation in conjugate formation that was dependent on target cell ICAM 1 expression , was blocked by LFA 1 alpha L or beta 2 chain specific antibodies , and occurred in the absence of Ui11 / E3 target cell exposure to DTT or quantitative changes in NK cell membrane LFA 1 expression . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Stromal cells and matrix expressed a wide variety of antigens of potential importance in haemopoietic cell adhesion , but no ICAM 1 , 2 or 3 could be demonstrated to correspond to the strong LFA 1 expression seen in haemopoietic precursor cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Lymphocyte function associated antigen 1 / intercellular adhesion molecule 1 ( LFA 1 / ICAM 1 ) and very late antigen 4 / vascular cell adhesion molecule 1 ( VLA 4 / VCAM 1 ) mediated adhesion of T lymphocytes to endothelial cells ( EC ) can be regulated by increased expression of ICAM 1 and VCAM 1 upon cytokine treatment of EC , or by activation of the integrin molecules LFA 1 and VLA 4 on T cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
FK 506 administration reduced markedly the incidence of infiltrating TCR alpha / beta + , CD5+ , CD4+ , CD8+ , and LFA 1 beta + cells and the expression both of MHC class 2 antigens and ICAM 1 on MNC , endothelial cells and thyrocytes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We have recently demonstrated that the adhesion of lymphocytes to cultured retroocular fibroblasts obtained from patients with Graves ' ophthalmopathy ( GO ) is mediated predominantly by the interaction of lymphocyte function associated antigen 1 ( LFA 1 ) , expressed on lymphocytes , with intercellular adhesion molecule 1 ( ICAM 1 ) , expressed by these cells following exposure to interferon gamma ( IFN gamma ) , tumour necrosis factor alpha ( TNF alpha ) , IL 1 alpha or purified thyroid stimulating immunoglobulins . ^^^ LFA 1 expressing , activated mononuclear cells and memory T lymphocytes ( CD3+ / CD45RO+ ) were only detected in GO retrocular tissues , and were mainly localized around blood vessels and in areas of ICAM 1 expressing connective and perimysial tissue . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
This ICAM 1 binding could be achieved by monovalent Fab fragments of mAb MEM 83 at concentrations equivalent to whole antibody , was associated with appearance of the `` activation reporter ' ' epitope detected by mAb 24 , and was completely inhibited by anti ICAM 1 and LFA 1 blocking mAbs . ^^^ These studies have therefore identified a novel LFA 1 activation epitope mapping to the 1 domain of LFA 1 , thereby implicating this domain in the regulation of LFA 1 binding to ICAM 1 . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The C 3 exoenzyme treatment affected neither protein phosphorylation in JY cells before and after PMA stimulation , nor affected surface expression of LFA 1 and ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
These findings suggest that , in IgG and IgA immune complex models of lung injury , both VLA 4 and ICAM 1 are required , although lymphocyte function associated Ag 1 is the predominant beta 2 integrin requirement in the IgG immune complex induced model of injury and Mac 1 is the predominant requirement for IgA immune complex induced lung injury . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The response to both cAMP and phorbol ester treatment distinguishes the regulation of CD 2 avidity from that of a second major adhesion pathway , LFA 1 ( CD11a / CD18 ) / ICAM 1 ( CD 54 ) and from that of the TCR coreceptor CD 8 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Role of LFA 1 / ICAM 1 and CD2 / LFA 3 adhesion molecules in CD 3 induced proliferative response . ^^^ As cord T cells , a model of antigen ( Ag ) unprimed cell , display a functional defect when stimulated through the CD 3 molecule , the role of lymphocyte function associated antigen 1 ( LFA 1 ) / intercellular adhesion molecule 1 ( ICAM 1 ) and CD2 / lymphocyte function associated antigen 3 ( LFA 3 ) receptor ligand pairs in cord CD 3 triggered T cell activation was analyzed using specific monoclonal antibodies ( mAb ) against each adhesion molecule . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In spite of down regulated expression of the adhesion molecules LFA 1 , LFA 3 , and / or ICAM 1 , peptide sensitized BL cells were recognized and lysed by EBV specific CTL . ^^^ Thus , down regulation of LFA 1 , LFA 3 , and / or ICAM 1 expression on BL cells does not provide an absolute barrier to tumor cell recognition by virus specific CTL . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Three different monoclonal antibodies ( MAb ) were used to assess the relative contributions of CD 18 ( MAb R15 . 7 ) , intercellular adhesion molecule 1 ( ICAM 1 ; MAb RR1 / 1 ) , and P selectin ( MAb PB1 . 3 ) to shear rate dependent leukocyte rolling and adherence . ^^^ The MAbs directed against either CD 18 or ICAM 1 , but not P selectin , significantly attenuated the recruitment of adherent leukocytes normally observed at low shear rates . ^^^ However , the MAb against P selectin , but not CD 18 or ICAM 1 , was effective in reducing the recruitment of rolling leukocytes elicited by low shear rates . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Further migration through the epidermis was mediated by expression on keratinocytes of intercellular adhesion molecule 1 ( ICAM 1 ) and of leukocyte function associated antigen 1 ( LFA 1 ) on infiltrating lymphocytes . ^^^ Further intraepithelial lymphocyte migration was then established , as already shown in the skin , by ICAM 1 / LFA 1 interaction . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
All cell lines from MS and controls expressed increased levels of LFA 1 ( CD11a ) , LFA 2 ( CD 2 ) , LFA 3 ( CD 58 ) , ICAM 1 ( CD 54 ) , and VLA 4 ( CDw49d ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
EXPERIMENTAL DESIGN : We analyzed the immunohistochemical localization of the adhesion molecules ( ICAM 1 , LFA 1 , Mac 1 , VCAM 1 , VLA 4 , P and E selectins ) in IBD , stressing phenotypical changes of endothelial cells . ^^^ RESULTS : In the normal mucosa , ICAM 1 was expressed in capillaries and venules , LFA 1 in some lymphocytes and VLA 4 in most lymphocytes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Proliferation is also inhibited by mAbs to T cell CD 2 and LFA 1 beta chain ( CD 18 ) and by mAbs to LFA 3 ( CD 58 ) and CD 44 , which are expressed by T cells and EC . mAb to ICAM 1 ( CD 54 , a ligand for LFA 1 ) provides inconsistent inhibition , and mAb to ICAM 2 , used with or without anti ICAM 1 , is not inhibitory . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Finally , plasma levels of sICAM 1 , but not sELAM 1 , were inversely correlated to the fraction of peripheral T cells having high surface expression of LFA 1 , the receptor for T cell adhesion to ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Until now , two pathways have been unravelled , i . e . , the LFA 1 / ICAM 1 and the VLA 4 / VCAM 1 pathways . ^^^ These findings suggest that the LFA 1 / ICAM 1 and VLA 4 / VCAM 1 pathways are involved in leucocyte migration in both types of lung disease , while in the active phases of sarcoidosis and UIP , ELAM 1 is also involved . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Cytolysis by CD4+ clones was blocked by antibodies to CD 4 and LFA 1 on T cells , and to ICAM 1 and class 2 molecules on astrocytes . ^^^ The role of LFA 1 in CD4+ cell mediated lysis was greater than that of LFA 1 / ICAM 1 in CD8+ T cell mediated lysis . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The expression and cellular distribution of adhesion molecules CD2 / LFA 3 and ICAM 1 / LFA 1 on mononuclear cells in squamous cell carcinoma of the head and neck . ^^^ The expressions and cellular distributions of two pairs of adhesion molecules CD2 / LFA 3 ( leukocyte function associated antigen 3 ) and LFA 1 / ICAM 1 ( intercellular adhesion molecule 1 ) were examined in inflammatory cellular infiltrates of advanced squamous cell carcinomas of the head and neck by immunohistochemical techniques including double staining methods . ^^^ Thirteen patients were investigated using the following monoclonal antibodies ( mAbs ) : CD 2 , LFA 3 ( CD 58 ) , ICAM 1 ( CD 54 ) , LFA 1 ( CD11a ) , the alpha / beta and gamma / delta T cell receptor , pan T cells and broadly distributed monocyte / macrophage ( m / m phi ) [ Fc gamma RII ( CD 32 ) , 25F9 , RM3 / 1 ] . ^^^ LFA 1 and ICAM 1 were expressed on lymphocytes as well as on m / m phi . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Cells with Langerhans ' cell histiocytosis morphology were stained for ICAM 1 , for the beta 1 integrins alpha 4 ( VLA 4 ) and alpha 5 ( VLA 5 ) , and for the beta 2 integrins LFA 1 , MAC 1 and p 150 , 95 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Adhesion molecules such as CD 2 and its ligand CD 58 ( LFA 3 ) , as well as CD11a / 18 ( LFA 1 ) and CD 54 ( ICAM 1 ) regulate not only cell to cell attachment but also participate in lymphocyte activation , recirculation , and effector function including cytolytic activity towards tumor cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Using flow cytometry , we also studied the expression of the adhesion molecules Mac 1 ( CD11b / CD18 ) and ICAM 1 ( CD 54 ) by monocytes and granulocytes after LPS stimulation . ^^^ Compared with phagocytes from healthy volunteers , CD 14 deficient cells showed poor Mac 1 and ICAM 1 upregulation when whole blood was stimulated with LPS ( 1 ng / ml ) , whereas their response to higher LPS doses ( 100 and 1 , 000 ng / ml ) was essentially normal . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In this study , we have characterized the divalent cation requirements for the adhesion of human peripheral CD4+ T cells to four distinct integrin ligands : the alpha 4 beta 1 and alpha 5 beta 1 ligand fibronectin , the alpha 4 beta 1 ligand VCAM 1 , the LFA 1 ligand ICAM 1 , and the alpha 4 beta 1 bacterial ligand invasin . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
U 937 cells treated with the phorbol ester PMA for 3 days adhere to both HIVEC and HIAEC ; this adhesion is mediated by LFA 1 interaction with ICAM 1 and / or 2 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Moreover , TNF alpha showed a co distribution with ICAM 1 expressed in the membranes of hepatocytes and with the occurrence of CD11b positive polymorphonuclear leukocytes ( neutrophils ) suggesting a possible major role of the beta 2 integrin Mac 1 as a ligand for ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
These results demonstrate that indomethacin induced leukocyte adherence and mucosal injury are dependent on the expression of CD 18 and ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The cytotoxic effect of CIK cells against tumor targets is blocked by antibodies directed against lymphocyte function associated antigen ( LFA 1 ) and its counter receptor , intercellular adhesion molecule 1 ( ICAM 1 ) . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies against CD11a , CD11b and CD 18 , and ICAM 1 , an adhesion molecule in the side of endothelial cells , abolished significantly the endothelial cell injury induced by PMA stimulated leukocytes . ^^^ The monoclonal antibodies against CD11a , CD11b , CD 18 , and ICAM 1 also showed inhibitory effects on the increase in intracellular fluorescence intensity of the endothelial cells exposed to PMA stimulated leukocytes . ^^^ Both the early increase in peroxide production and late cell lysis were abolished by specific antibodies against CD11a , CD11b , CD 18 , and ICAM 1 , but not CD11c . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule 1 ( ICAM 1 ) is a ligand for lymphocyte function associated antigen 1 alpha ( LFA 1 alpha ) and has been shown to be required for leukocyte migration into inflamed area . ^^^ The purpose of this report was to investigate the role of ICAM 1 / LFA 1 alpha pathway in a rat model of extrinsic asthma using monoclonal antibodies ( mAbs ) . ^^^ The administration of mAbs to ICAM 1 and LFA 1 alpha significantly attenuated the ER ( RL , Rti , Raw = 146 + / 9 , 141 + / 11 , 156 + / 8 % baseline ) and LR ( RL , Rti , Raw = 128 + / 8 , 124 + / 5 , 137 + / 1 % baseline ) , indicating that both airway and lung tissues were involved in this mechanism . ^^^ The current observations suggest that ICAM 1 / LFA 1 alpha pathway is involved in both the early and late responses in a rat model of allergic asthma . ^^^ The antagonism of ICAM 1 and LFA 1 alpha may provide a potential therapeutic approach to the early and late responses of bronchial asthma . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In addition , they express Thy 1 . 1 , LFA 1 , ICAM 1 , and the gp 23 epithelial antigen , and synthesize laminin . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The IFN gamma exerted its effect by up regulating both MHC class 2 and intercellular adhesion molecule 1 ( ICAM 1 ) expression by these cells as neutralizing antibodies to Ia , LFA 1 and ICAM 1 inhibited presentation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The binding was inhibited by monoclonal antibodies against LFA 1 and , to a lesser extent , ICAM 1 , thus demonstrating the importance of these molecules in the observed binding ; neither anti VCAM 1 nor anti E selectin antibodies affected the binding . ^^^ From these various data , we conclude that LFA 1 / ICAM 1 interactions are partially responsible for the binding of lymphocytes to endothelial cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To show that inhibition by ECCD 2 was specific for adhesion mediated by alpha M 290 beta 7 , MTC 1 cells were induced to adhere to CMT 93 via the LFA 1 / ICAM 1 pathway . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Furthermore , a batch of other cellular molecules including LFA 1 , ICAM 1 , LFA 3 , MHC 1 , MHC IIDR , and CD 4 became detectable on HTLV 1 binding BW 5147 cells , a mouse T lymphoma cell line , which were able to adsorb HTLV 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Involvement of LFA 1 and ICAM 1 in the herpetic disease resulting from HSV 1 corneal infection . ^^^ These studies investigated the involvement of the adhesion pair LFA 1 / ICAM 1 in the disease that results from HSV 1 corneal infection in mice . ^^^ Both LFA 1 and ICAM 1 appear to be important for protection of the skin from HSV 1 infection . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Antibody blocking studies of the ligands on HUVE indicated that E selectin may be partially involved in this CD 18 independent PMNL migration but that ICAM 1 , VCAM 1 , PECAM 1 , and P selectin are not involved . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
These data suggest that the inhibitory effect of IL 2 activated natural killer cells on clonogenic AML cells is mediated principally via the lymphocyte function associated molecule 1 ( LFA 1 ) / ICAM 1 interaction . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We evaluated the ability of monoclonal antibodies directed against leukocyte adhesion molecules ( intercellular adhesion molecule 1 [ ICAM 1 ] , CD 18 ) to enhance the efficacy of thrombolysis in a rabbit cerebral embolism stroke model . ^^^ Neither alpha CD 18 nor anti ICAM 1 ( alpha ICAM 1 ) improved neurologic outcome at postischemic delays of 15 or 30 minutes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Suppression of corneal allograft rejection after penetrating keratoplasty by antibodies to ICAM 1 and LFA 1 in mice . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Kinetics of human T cell expression of LFA 1 , IL 2 receptor , and ICAM 1 following antigenic stimulation in vitro . ^^^ We show that the well established differences in kinetics of mitogen and antigen induced T cell proliferation in vitro is paralleled by differential kinetics in the expression of the T cell adhesion and activation antigens leukocyte function associated antigen 1 ( LFA 1 ; CD11a / CD18 ) , interleukin 2 receptor ( IL 2R ; CD 25 ) , and intercellular adhesion molecule 1 ( ICAM 1 ; CD 54 ) . ^^^ While this is in agreement with previous reports of the expression kinetics of IL 2R and ICAM 1 , this is the first report indicating that the regulation of T cell surface expression of LFA 1 is bidirectional . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Natural killer ( NK ) cells and CD8+ cytotoxic T lymphocytes ( CTL ) were shown to lyse C 1498 in vitro through the binding of leukocyte function antigen 1 ( LFA 1 ) on effectors and intercellular adhesion molecule 1 ( ICAM 1 ) and ICAM 2 on C 1498 target cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In addition , these cells were weakly positive for CD11b ( Mac 1 ) and CD 54 ( ICAM 1 ) , but were negative for lymphocyte specific CD11a ( LFA 1 ) and CD11c ( p 150 , 95 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The other leukocyte integrins , Mac 1 and p 150 , 95 , also interact with ICAM 1 . ^^^ ICAM 1 and ICAM 3 are highly homologous , and an undefined ligand for Mac 1 is present on neutrophils where ICAM 3 is well expressed . ^^^ In addition , glycosylation has been shown to affect the interaction of ICAM 1 with Mac 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Induction of allogeneic T cell proliferation by NS cells was mediated by HLA DR , leukocyte function associated antigen 1 ( LFA 1 ) , LFA 3 , B 7 1 / CD80 , and CTLA 4 and resulted in the upregulation ( LFA 3 , intercellular adhesion molecule 1 [ ICAM 1 ] , CD 40 ) and induction ( B 7 1 / CD80 , B 7 2 / CD86 , L16 / activated LFA 1 ) of accessory molecules on NS cells . ^^^ In turn , responder T lymphocytes were induced to express B 7 1 / CD80 , B 7 2 / CD86 , CD 40 ligand ( CD40L ) , ICAM 1 , L16 / activated LFA 1 , and HLA DR , reflecting bidirectional signaling between T lymphocytes and B NHL cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Role of anti LFA 1 and anti ICAM 1 combined MAb treatment in the rejection of tumors induced by Moloney murine sarcoma virus ( M MSV ) . ^^^ We investigated the effect of combined treatment with anti LFA 1 and anti ICAM 1 monoclonal antibodies ( MAbs ) in the immune reaction to Moloney murine sarcoma virus ( M MSV ) induced tumors , which spontaneously regress due to the generation of a strong virus specific cytotoxic T lymphocyte ( CTL ) response . ^^^ However , anti LFA 1 MAb , alone or in combination with anti ICAM 1 MAb , prevented lymphocyte homing in tumor draining lymph nodes . ^^^ Cytofluorimetric analysis disclosed a profound down modulation of LFA 1 and ICAM 1 molecule expression on T cells following in vivo MAb treatment . ^^^ Moreover , in anti LFA 1 MAb treated mice , the receptor was coated to saturation , while anti ICAM 1 MAb treatment brought about ICAM 1 molecule coating levels below saturation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Eyes ( three infected , three uninfected ) were embedded in OCT compound , frozen in liquid nitrogen , sectioned on a cryostat , and stained for ICAM 1 and LFA 1 immunoreactivity . ^^^ Conversely , the intensity of immunostaining for LFA 1 , a ligand for ICAM 1 on infiltrating leukocytes , was similar despite animal age . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
These markers consist of T cell specific antigens ( CD 2 , CD 3 , CD 4 , and CD 8 ) , activated T cell antigens ( CD 25 and CD 71 ) , major histocompatibility complex ( MHC ) antigens ( class 1 and 2 ) , and adhesion molecules ( LFA 1 and ICAM 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Expression of intercellular adhesion molecules , such as ICAM 1 and LFA 1 , was higher in PE than in PB . ^^^ It is of interest that the expression of ICAM 1 and LFA 1 on T cells in PB was significantly higher in group B than in group A . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Serial 2 microns sections were stained with chromotrope 2R and MoAbs against ICAM 1 , CD11a / CD18 ( LFA 1 ) and CD 3 . ^^^ Af challenged mice presented significant increases in eosinophil , T lymphocyte and LFA 1 positive cell count and up regulated expression of ICAM 1 in the lung tissue at all the time points examined . ^^^ ICAM 1 expression intensity correlated with the number of T lymphocytes ( r = 0 . 59 , P < 0 . 01 ) , LFA 1 positive cells ( r = 0 . 68 , P < 0 . 001 ) , but not of eosinophils ( r = 0 . 24 , P > 0 . 05 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
MoAbs in immunoperoxidase were used to identify the expression of ICAM 1 and VCAM 1 on endothelial cells ( EC ) , and of their ligands LFA 1 and VLA 4 on lymphocytes and accessory cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
METHODS Using an indirect immunoperoxidase technique we assessed the expression of the cytokines interleukin 1 alpha ( IL 1 alpha ) , interleukin 1 beta ( IL 1 beta ) , interleukin 8 ( IL 8 ) , transforming growth factor beta ( TGF beta ) and granulocyte macrophage colony stimulating factor ( GM CSF ) , of the adhesion molecules intercellular adhesion molecule 1 ( ICAM 1 ) , lymphocyte function associated antigen 1 ( LFA 1 ) , the activated molecular form of LFA 1 ( NKI L 16 ) , CD 2 , and LFA 3 , and of a panel of cellular markers in the minor salivary glands . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We investigated the expression of intercellular adhesion molecule 1 ( ICAM 1 ) and lymphocyte function associated antigen 1 ( LFA 1 ) by cells in the central nervous system ( CNS ) of Lewis rats during acute experimental allergic encephalomyelitis ( EAE ) . ^^^ An increase in either ICAM 1 or LFA 1 positive cells was initially seen in the lumbosacral portion of the spinal cord , which then extended to the thoracic portion . ^^^ The number of either ICAM 1 or LFA 1 positive cells peaked on the day of clinical onset in the lumbosacral portion . ^^^ In contrast , in the thoracic portion , a peak in the number of either ICAM 1 or LFA 1 positive cells was observed on the day after clinical onset . ^^^ This ascending extension of either ICAM 1 or LFA 1 positive cells was correlated with the progression of neurologic signs . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Additionally , rats were passively immunized with neutralizing antibodies to TNF , ENA 78 , MIP 1 alpha , JE / MCP 1 , intercellular adhesion molecule 1 ( ICAM 1 ) , and CD 18 compared with control antibodies . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Follicular thyroid cells of autoimmune thyroiditis may coexpress ICAM 1 ( CD 54 ) and its natural ligand LFA 1 ( CD11a / CD18 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Furthermore , all alternative isoforms of ICAM 1 , except one , retain the ability to bind to the well recognized ICAM 1 counter receptor LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Involvement of adhesion molecules LFA 1 and ICAM 1 in osteoclast development . ^^^ We report here that leukocyte function associated antigen 1 ( LFA 1 ) and intercellular adhesion molecule 1 ( ICAM 1 ) are involved in osteoclast development . ^^^ When either one of the monoclonal antibodies ( MAbs ) to mouse LFA 1 and mouse ICAM 1 was added to the co culture system , the TRACP activity was significantly inhibited . ^^^ These results indicate that adhesion molecules LFA 1 and ICAM 1 may play a role in osteoclast development via interaction between stromal cells and osteoclast progenitors as well as among osteoclast progenitors . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Using a panel of monoclonal antibodies the following results were obtained : ( 1 ) ICAM 1 was expressed on epidermal keratinocytes , dermal inflammatory cells , and endothelial cells in most biopsies , whereas LFA 1 was confined to the dermis . ^^^ Attachments between keratinocytes or endothelial cells and activated T lymphocytes via ICAM 1 / LFA 1 may be a possible mechanism of target / effector recognition in cutaneous LE . ( 2 ) HLA DR was expressed on epidermal keratinocytes and cells of the dermal infiltrate , but not on endothelial cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Residues critical for human ICAM 1 binding to the beta 2 integrin leukocyte function associated antigen 1 ( LFA 1 ) are highly conserved between all species , whereas those residues demonstrated to play an important role in interaction of human ICAM 1 with macrophage activation complex 1 ( Mac 1 ) are not highly conserved . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Fifty to ninety percent of lung and BAL T cells from vehicle sensitized or OA sensitized and challenged mice expressed the adhesion molecules CD11a ( LFA 1 ) , CD 54 ( ICAM 1 ) , and CD49d ( VLA 4 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The upregulation of both adhesion molecules , ICAM 1 and Mac 1 , is necessary for a neutrophil induced liver injury to occur . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Protective effect of monoclonal antibodies against LFA 1 and ICAM 1 on myocardial reperfusion injury following global ischemia in rat hearts . ^^^ The effects of anti LFA 1 and anti ICAM 1 monoclonal antibodies ( MAbs ) on the reperfusion injury of rat cardiac tissues after global ischemia were studied . ^^^ Isolated rat hearts were perfused with blood from an anesthetized support rat with or without anti LFA 1 or anti ICAM 1 monoclonal antibody administration ( n = 10 in each group ) . ^^^ Treatment with anti LFA 1 MAb or anti ICAM 1 MAb resulted in a 77 . 2 + / 1 . 5 % and a 80 . 4 + / 3 . 0 % recovery of LVDP , respectively . ^^^ In addition , increase in coronary vascular resistance was only 23 + / 7 % and 13 + / 6 % in anti LFA 1 and anti ICAM 1 treated groups , respectively . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
This liver associated lymphocyte population also presented a significantly higher percentage of Mac 1 , ICAM 1 , and LFA 3 and an increased surface expression of LFA 1 , LFA 2 , and NCAM in comparison with peripheral blood lymphocytes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Three counter receptors for LFA 1 of the immunoglobulin family have been discovered : ICAM 1 , ICAM 2 , and ICAM 3 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
When the expression pattern of adhesion molecules , including CD 44 , L selectin , LFA 1 and ICAM 1 , was compared among these T cell populations , they displayed quite unique patterns of expression . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies against ICAM 1 and LFA 1 prolong nerve allograft survival . ^^^ After engraftment , recipients were treated with antiintercellular adhesion molecule 1 ( ICAM 1 ) and antilymphocyte function associated antigen 1 ( LFA 1 ) MAbs for 14 days . ^^^ MAbs therapy directed against ICAM 1 / LFA 1 presents a new approach for the management of the peripheral nerve allograft response . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Similarly , blood derived human phagocytes were stimulated to express adhesion molecules ( LFA 1 , MAC 1 , p 150 , 95 , ICAM 1 ) , to synthesize TNF alpha and IL 2 , and to develop a natural killer activity . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We histochemically examined the expression of adhesion molecules , ICAM 1 and LFA 1 , of endothelial cells in the synovial tissues of rheumatoid arthritis . ^^^ ICAM 1 was expressed on the cell surface and in the cytoplasm of the endothelial cells of small veins , and in the germinal center of lymphoid follicle , while LFA 1 was expressed on lymphocytes around the small vessels which expressed the ICAM 1 molecule . ^^^ At one month from onset , faintly positive ICAM 1 molecules were expressed on endothelial cells accompanied by LFA 1 positive spindle cells , called interstitial cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Adhesion was also unaffected by antibodies to LFA 1 , ICAM 1 , ICAM 2 , LFA 2 , or LFA 3 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Immunohistochemical study under light microscopy demonstrated that all of the mononuclear cells infiltrating the islets strongly expressed ICAM 1 and leukocyte function associated antigen 1 ( LFA 1 ) , a counterreceptor of ICAM 1 , whereas ICAM 1 expression on islet cells was not apparent . ^^^ Susceptibility of MIN6N8a cells to lysis by a NOD islet derived CD8+ cytotoxic T cell clone was greatly enhanced by IFN gamma pretreatment , and this enhancement was abolished by anti ICAM 1 and anti LFA 1 mAbs . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
BACKGROUND : Increased expression of intercellular adhesion molecule 1 ( ICAM 1 ) and lymphocyte function associated antigen 1 ( LFA 1 ) has been reported to play a major role in reperfusion injury after ischemia . ^^^ METHODS : Histological changes and expression of ICAM 1 and LFA 1 were investigated in rat partial liver warm ischemia , performed by clamping hepatic hilar vessels distributing to the right lateral lobe for 30 , 45 , and 60 minutes . ^^^ RESULTS : Reperfusion after 60 minutes of warm ischemia induced histological liver injury and strong expression of ICAM 1 and LFA 1 , although 30 and 45 minutes of ischemia did not provoke either histological damages or the expression of ICAM 1 and LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The analyzed molecules included the beta 1 integrin or VLA family VLA 1 6 , the beta 2 integrins or leukocyte integrins LFA 1 , Mac 1 , and p 150 , 95 , the immunoglobulins LFA 3 , CD 2 , ICAM 1 and VCAM 1 and the selectins ELAM 1 and GMP 140 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The effect of treatment with monoclonal antibody ( MoAb ) 1A29 against rat intercellular adhesion molecule 1 ( ICAM 1 ) alone and a mixture of MoAb 1A29 and MoAb WT 3 against rat CD 18 on asthmatic responses of the rats was studied . ^^^ These results show that eosinophil infiltration corresponds closely to bronchoconstriction in LAR and that treatment with MoAbs to ICAM 1 and CD 18 may be effective in reducing asthmatic symptoms . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Although it is known that the neutrophil adhesion receptor ( CD 18 ) plays no role in determining PMN accumulations in the region aspirated , we postulated that this PMN adhesion receptor and its endothelial ligand , intercellular adhesion molecule 1 ( ICAM 1 ) , mediate remote neutrophil sequestration . ^^^ Acid aspiration leads to neutrophil adhesion and edema in regions remote from those aspirated via neutrophil CD 18 and endothelial ICAM 1 . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Neutrophil HT 29 cell adhesion was blocked by monoclonal antibodies to neutrophil CD 18 or to ICAM 1 on the HT 29 cells ( 86 % and 56 % inhibition , respectively , P < 0 . 01 for both ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Recombinant alpha L beta 2 ( LFA 1 , CD11a / CD18 ) and alpha M beta 2 ( MAC 1 , CD11b / CD18 ) were expressed on COS cells , and function was assessed by adhesion to ICAM 1 or iC3b , respectively . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We therefore investigated the roles of CD11a / CD18 ( LFA 1 ) and ICAM 1 in cerebral ischemia reperfusion injury by using monoclonal antibodies , WT 1 ( anti CD11a ) , WT 3 ( anti CD 18 ) , and 1A29 ( anti ICAM 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
ICAM 1 / LFA 1 interactions in T lymphocyte activation and adhesion to cells of the blood retina barrier in the rat . ^^^ Forty to 48 % of unactivated T cells were found to bind to normal REC or RPE by leucocyte function associated antigen 1 / intercellular adhesion molecule 1 ( LFA 1 / ICAM 1 ) independent mechanisms , despite constitutive expression of ICAM 1 by the RPE cells and LFA 1 by the T cells . ^^^ Activated lymphocyte adhesion to unstimulated RPE and REC was significantly blocked by LFA 1 mAb ( 35 % , P < 0 . 0001 ) and ICAM 1 mAb ( 20 % , P < 0 . 001 ) . ^^^ Both ICAM 1 and LFA 1 mAb also significantly ( P < 0 . 05 ) blocked antigen presentation following retinal extract stimulation of lymphocytes from immunized rats in proliferation assay . ^^^ These data suggest that the ICAM 1 / LFA 1 system is important in lymphocyte trafficking into the eye only after lymphocyte activation . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
No inhibition of binding was seen with antibodies to intracellular adhesion molecule 1 ( ICAM 1 ) or lymphocyte function associated antigen 1 ( LFA 1 ) . ^^^ Preincubation of endothelial cells with plasma isolated from the portal or hepatic vein of normal adult mice ( 5 % plasma , 37 degrees for 14 hr ) caused a 1 . 5 2 fold stimulation of binding of MLN / PP to heart endothelium , which was inhibited ( > or = 75 % ) by anti ICAM 1 or anti LFA 1 , and a fourfold stimulation of binding to liver endothelium , which was not inhibited by these monoclonal antibodies ( < or = 25 % inhibition ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
LFA 1 / ICAM 1 interaction is important in facilitating a number of cellular events including antigen specific T cell activation and leukocyte transendothelial migration . ^^^ We are interested in defining residues and contact sites that mediate ICAM 1 interaction with the integrin receptor , LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Using activated umbilical endothelial cells , we showed that LAD 1 neutrophil do not bind to cells expressing ICAM 1 , while LAD 2 cells do not bind to endothelial cells expressing E or P selectin . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
GM CSF augmented the surface expression of ICAM 1 and CD 18 in TAM and in blood monocytes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
HCA occurred at an optimal temperature of 37 C but not 4 C . mAbs to CD 18 ( lymphocyte function associated antigen 1 beta ; LAF 1 beta ) and CD 54 ( intercellular adhesion molecule 1 ; ICAM 1 ) completely inhibited this HCA . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Murine blood DC expressed similar levels of LFA 1 , LECAM 1 and CD 44 compared with lymph node DC but lower levels of MHC class 2 , B 7 and ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
It was found that a number of adhesion molecules , such as CD 2 , CD 4 , CD 8 , LFA 1 , CD 18 , ICAM 1 and Thy 1 , was partly involved in the nursing activity . ^^^ The inhibitory effect of mAb to CD 2 , LFA 1 , CD 18 and ICAM 1 on thymocyte engulfment was higher than their effect on thymocyte binding to the R TNC . 1 line . ^^^ In addition , a LFA 1 / CD18 dependent / ICAM 1 independent adhesion pathway was identified when unstimulated R TNC . 1 cells with minimal expression of ICAM 1 were used . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Our studies demonstrate that CLA+ T cells use LFA 1 / intercellular leukocyte adhesion molecule 1 ( ICAM 1 ) for transmigration but that CLA expression is not required for the LFA 1 / ICAM 1 dependent transmigration because anti CD18 / CD11a mAbs and anti ICAM 1 mAbs were able to block T cell migration regardless of the activation state of HUVEC or the CLA expression by T cells . ^^^ Taken together , our results suggest that CLA has a homing function in conducting the T cell to interact with LFA 1 / ICAM 1 and / or VLA 4 / VCAM 1 ; this results in enhanced adhesion and migration across cytokine activated endothelial cells . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The intercellular adhesion molecule 3 ( ICAM 3 ) has been identified as the third LFA 1 ligand in addition to ICAM 1 and ICAM 2 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Changes of adhesion molecule ( LFA 1 , ICAM 1 ) expression on memory T cells activated with cytomegalovirus antigen . ^^^ The expression of adhesion molecules ( LFA 1 , ICAM 1 , CD 29 ) on T cells activated with cytomegalovirus ( CMV ) antigen was investigated by three color flow cytometry analysis . ^^^ After culture , the expression of LFA 1 , ICAM 1 , and CD 29 on T cells in subpopulations defined as CD45RO+ or CD45RO was analyzed . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
LFA 1 integrin redistribution during T cell hybridoma invasion of hepatocyte cultures and manganese induced adhesion to ICAM 1 . ^^^ For optimal adhesion to ICAM 1 , LFA 1 on T cell hybridomas requires activation , which apparently occurs upon contact with cell layers that are invaded ( G . ^^^ To study LFA 1 redistribution upon ICAM 1 interaction with higher resolution , we performed immuno EM on cells before and after Mn ( 2+ ) induced adhesion and spreading on immobilized ICAM 1 . ^^^ By immune fluorescence , LFA 1 was observed to redistribute to the ICAM 1 adherent surface , and to be concentrated in lamellipodia of spreading TAM8C4 cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In addition to these activities LFA 1 and ICAM 1 are implicated in the cell to cell transmission of human immunodeficiency virus ( HIV 1 ) since antibodies to CD 18 , CD 54 or synthetic peptide analogs of ICAM 1 antagonise the formation of virus induced syncytia ( Fecondo et al . , 1993 ; Gruber et al . , 1991 ; Hildreth and Orentas , 1989 ; Valentin et al . , 1990 ) . ^^^ The intercellular adhesion molecule ( ICAM 1 , CD 54 ) and its counter receptor , the integrin leukocyte function associated antigen 1 ( LFA 1 , CD11a / CD18 ) , have important roles in the immune response . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The addition of NeuAc2en during LPS stimulation did not affect the surface expression of Ia , LFA 1 , ICAM 1 or mB 7 , suggesting that inhibition of LPS mediated enhancement by the sialidase inhibitor was not due to changes in the level of expression of the major B cell adhesion or co stimulatory molecules . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Cardiac allografts in rat recipients with simultaneous use of anti ICAM 1 and anti LFA 1 monoclonal antibodies leads to accelerated graft loss . ^^^ Monoclonal antibodies to rat ICAM 1 or LFA 1 were administered intravenously to WKAH rats receiving Fisher cardiac allografts for 3 7 consecutive days after transplantation . ^^^ Surprisingly , simultaneous use of both anti ICAM 1 and anti LFA 1 antibodies resulted in accelerated graft loss with interstitial edema , diffuse myocardial degeneration , vascular injury and scattered hemorrhage . ^^^ Thus , beneficial effects on allograft survival were partially seen when recipients were given anti ICAM 1 or anti LFA 1 antibody alone . ^^^ However , serious disadvantage in preventing rejection was clearly seen when both anti ICAM 1 and anti LFA 1 antibodies were used together . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
LFA 1 molecules were seen as granules on the surfaces of all infiltrates , most of which also expressed ICAM 1 . ^^^ In LR , ICAM 1 and HLA DR were expressed only sparsely on the undersurface of the epidermis , whereas the infiltrates expressed LFA 1 molecules on their surfaces . ^^^ These findings may explain the differing histological features of MF and LR : ICAM 1 molecules present on the intercellular junctions of MF epidermis lead the LFA 1 bearing cells to migrate into the interspaces of the keratinocytes , thus producing `` epidermotropism ' ' . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Strong expression of the adhesion molecules LFA 1 and ICAM 1 is present on infiltrating cells as well as on resident cells of the organ . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies against rat intercellular adhesion molecule 1 ( ICAM 1 ) and CD 18 of beta 2 integrins administered both 5 min before a period of 45 min renal ischaemia ( 20 micrograms / kg i . v . ) and at the commencement of 1 h reperfusion ( 20 micrograms / kg i . v . ) reduced renal tissue polymorphonucleocyte accumulation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Involvement of ICAM 1 / LFA 1 adhesion molecule set in rat orthotopic liver transplantation ] . ^^^ The distribution of ICAM 1 / LFA 1 positive cells in rat liver grafts was investigated . ^^^ Transplanted liver tissues on days 2 , 4 and 7 were immunohistochemically stained , using mouse anti rat ICAM 1 monoclonal antibody ( 1A29 , IgG 1 ) and mouse anti rat LFA 1 monoclonal antibody ( WT 1 , IgG2a ) . ^^^ ICAM 1 upregulation on target structures of liver graft rejection suggests the importance of ICAM 1 / LFA 1 in the pathogenesis of allograft rejection . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In vitro binding of MNC from naive animals to chronically rejecting allografted kidneys generally confirmed the immunohistological observations , peaking at 12 wk ; this binding was significantly inhibited by mAbs against specific adhesion molecules ( CD11a , CD 18 , and ICAM 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Role of ICAM 1 and LFA 1 in a cardiac xenograft rejection model . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
This adhesion is mediated by the LFA 1 alpha / beta = ICAM 1 , the VLA 4 = VCAM 1 , and the ICAM 1 = C3bi receptor ligand pathways on B cells and on FDC , respectively . ^^^ The loss of LFA 1 alpha / beta and ICAM 1 molecules may enable neoplastic lymphocytes to detach from FDC . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Because a prominent polymorphonuclear infiltrate was observed in the lesions at the time of maximal tissue swelling , we investigated the involvement of polymorphonuclear leukocytes and their adhesion molecules lymphocyte function associated antigen 1 ( LFA 1 ) and intercellular adhesion molecule 1 ( ICAM 1 ) in Th 2 cell mediated inflammation . ^^^ Pretreatment of recipient mice with a depleting monoclonal antibody to neutrophils or with blocking antibodies to LFA 1 or to ICAM 1 completely abrogated tissue swelling in Th 2 cell mediated inflammation . ^^^ Granulocyte infiltration at 6 h was also inhibited by the antibodies to neutrophils and to ICAM 1 , but not by that to LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The myeloma cells were positive for CD 33 , CD 45 , CD45RA , CD 63 , CD 71 , plasma cell associated antigens such as CD 38 , PCA 1 , BL 3 , and various kinds of adhesion molecules : CD11a / CD18 ( LFA 1 ) , CD 29 ( VLA beta 1 ) , CD 44 ( H CAM ) , CD49d ( VLA 4 ) , CD 54 ( ICAM 1 ) , CD 56 ( N CAM ) , CD 58 ( LFA 3 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In vivo , keratinocytes , which are the first target cells , can be directly activated to participate in the local reaction , especially through the expression of the membrane antigen ICAM 1 , a ligand of the leucocyte antigen LFA 1 , and the production of cytokines . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
MHC class 2 antigen ( Ia ) , intercellular adhesion molecule 1 ( ICAM 1 ) and lymphocyte function associated antigen 1 ( LFA 1 ) . ^^^ The phenotypes found in these rats were as follows : T cells ( 70 . 3 % ) ; CD 4 + ( 58 . 3 % ) ; CD 8 + ( 40 . 3 % ) ; Ia ( 51 . 9 % ) ; ICAM 1 ( 62 . 6 % ) ; LFA 1 ( 75 . 8 % ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
ICAM 1 is the natural ligand for the LFA 1 integrin expressed at the surface of lymphoid cells . ^^^ ICAM 1 / LFA 1 interactions favour homotypic and heterotypic cell cell adhesion . ^^^ Since human fibroblasts express an LFA 1 like molecule , we propose that in these cells IL 2 can modify homotypic and heterotypic interactions acting on the surface expression of ICAM 1 protein . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies against ICAM 1 and LFA 1 ( CD11A ) induce specific tolerance to peripheral nerve allograft in rats . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Role of intercellular adhesion molecule 1 ( ICAM 1 ) and lymphocyte function associated antigen 1 ( LFA 1 ) in peripheral blood mononuclear cell activation by human renal carcinoma cells . ^^^ We examined the role of intercellular adhesion molecule 1 ( ICAM 1 ) and lymphocyte function associated antigen 1 ( LFA 1 ) during the activation of peripheral blood mononuclear cells ( PBMCs ) by the human renal carcinoma cell line CaKi 1 . ^^^ Furthermore , the induced PBMC proliferation was inhibited by specific monoclonal antibodies against ICAM 1 and LFA 1 . ^^^ We conclude that PMA can modulate CaKi 1 cells to stimulate allogenic PBMC proliferation in an ICAM 1 / LFA 1 dependent , but HLA A , B , C independent , fashion . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The biological significance of the signals triggered by the interaction of cell surface expressed LFA 1 and ICAM 1 has been investigated in Con A and immobilized anti CD 3 mAb stimulated cultures . ^^^ When added at the beginning of activation in the presence of Con A , soluble anti LFA 1 and anti ICAM 1 mAbs could strongly inhibit cell proliferation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In particular , we evaluated the surface expression and functional activity of intercellular adhesion molecule 1 ( ICAM 1 ) , a cell surface glycoprotein that has been identified as one of the ligands for lymphocyte function associated antigen 1 ( LFA 1 ) , present on almost all leucocyte cell types . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Venular endothelium in the skin lesions expressed intercellular adhesion molecule 1 ( ICAM 1 ) , which is the ligand for LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We investigated immunohistochemically the expression of platelet endothelial cell adhesion molecule 1 ( PECAM 1 ) , intercellular adhesion molecule 1 ( ICAM 1 ) and lymphocyte function associated antigen 1 ( LFA 1 ) on resected specimens taken at a distance of 2 4 cm from the inflamed area and without histological signs of inflammation . ^^^ Compared with normal gut , we found ( 1 ) a significant increase of PECAM 1 positive vessels in the mucosa of uninvolved UC ( 149 . 0 + / 24 . 1 vessels / mm2 ( mean + / s . d . ) ; normal colon = 123 . 1 + / 21 . 6 ; P = 0 . 004 ) ; ( 2 ) a significant decrease of ICAM 1 positive vessels in uninvolved CD ( 111 . 9 + / 22 . 6 vessels / mm2 ; normal ileum = 136 . 9 + / 27 . 6 ; P = 0 . 04 ) ; and ( 3 ) a moderate but statistically insignificant increase of LFA 1 positive cells in the mucosa of uninvolved UC and Crohn ' s ileitis . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The extract induced adhesion was significantly diminished by monoclonal antibodies ( MAb ) directed against either CD11a , CD11b , or CD 18 on neutrophils , and by MAbs against intercellular adhesion molecule 1 ( ICAM 1 ) , but not E or P selectin , on endothelial cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
ICAM 3 interacts with LFA 1 and regulates the LFA 1 / ICAM 1 cell adhesion pathway . ^^^ ICAM 3 mediated cell aggregation was dependent on the LFA 1 / ICAM 1 pathway as demonstrated by blocking experiments with mAbs specific for the LFA 1 and ICAM 1 molecules . ^^^ Furthermore , immunofluorescence studies on ICAM 3 induced cell aggregates revealed that both LFA 1 and ICAM 1 were mainly located at intercellular boundaries . ^^^ Experiments of T cell adhesion to a chimeric ICAM 1 Fc molecule revealed that the proaggregatory anti ICAM 3 HP2 / 19 mAb was able to increase T lymphoblast attachment to ICAM 1 , suggesting that T cell aggregation induced by this mAb could be mediated by increasing the avidity of LFA 1 for ICAM 1 . ^^^ Altogether , our results indicate that ICAM 3 has a regulatory role on the LFA 1 / ICAM 1 pathway of intercellular adhesion . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The expression of ICAM 1 and LFA 1 was increased during activation and anti LFA 1 antibody blocked APC function . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In summary , we postulate that both LFA 1 / ICAM 1 , and CD2 / LFA 3 interactions are involved in EC accessory function , although the role of the EC associated adhesion partners is not fully understood . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Furthermore , ICAM 1 , which is the ligand for LFA 1 molecules , was also expressed on eosinophils from BA patients . ^^^ To extend our understanding of this high intensity expression of adhesion molecules ( CR 3 , LFA 1 alpha , LFA 1 beta , ICAM 1 ) on such eosinophils , we examined whether chemotactic agents might be involved in adhesion molecule expression using an eosinophilic cell line named EoL 3 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule 1 ( ICAM 1 ) is one of 3 major ligands for the beta 2 integrin leucocyte function associated antigen 1 ( LFA 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Role of LFA 1 / ICAM 1 in interleukin 2 stimulated lymphocyte proliferation . ^^^ Major adhesion routes between lymphoid cells involve the receptor / ligand pairs LFA 1 / ICAM 1 and CD2 / LFA 3 , in addition to VLA or CD 44 molecules . ^^^ Selective inhibition of recombinant ( r ) IL 2 induced cell proliferation was observed with mAb directed against the alpha or beta subunit of LFA 1 or to its ligand ICAM 1 . ^^^ By using mAb that specifically could block distinct rIL 2 activation pathways , LFA 1 / ICAM 1 interaction was found to be required for p 55 IL 2 receptor ( IL 2R ) mediated interaction of rIL 2 with its high affinity receptor , but not for p 75 IL 2R mediated responses . ^^^ Furthermore , it was shown that the rIL 2 response of T lymphocytes , but not of natural killer cells , was dependent on LFA 1 / ICAM 1 interaction . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Our results demonstrate that whereas expression of major histocompatibility complex ( MHC ) molecules , CD 45 , Mac 1 , LFA 1 , B cell markers , macrophage markers and some T cell markers ( as CD 2 , CD 4 and CD 8 ) did not undergo changes in culture , the level of expression of the adhesion molecules VLA 4 and ICAM 1 , and the T cell markers CD 5 , CD 25 and Thy 1 increased after 14 h incubation at 37 degrees C . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Antibodies to two different leukocyte molecules , CD11a and CD 18 , and antibodies to the endothelial ligand for CD11a / CD18 , ICAM 1 ( intercellular adhesion molecule 1 , CD 54 ) , were studied in inflammation after intravitreally injected interleukin 1 , intravitreally injected endotoxin , or an ocular reversed passive Arthus reaction . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Requirements for activating LFA 1 adhesion to ICAM 1 are similar to those found for induction of binding to immobilized fibronectin ( FN ) , but distinct from those for activating CD 8 mediated adhesion to class 1 major histocompatibility complex . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Cultures treated with monoclonal antibodies to the ICAM 1 and LFA 1 adhesion molecules showed an impaired release of both IFN types after gp 120 stimulation , suggesting a crucial role of cell to cell interactions in the process leading to IFN production . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
A circulating form of the membrane bound ICAM 1 ( CD 54 ) , a ligand for lymphocyte function associated antigen 1 ( LFA 1 ) , has recently been identified in normal human serum . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To examine the role played by adhesion molecules in thyroid autoimmunity , we have assessed the effect of administering monoclonal antibodies ( mAb ) against intercellular adhesion molecule 1 ( ICAM 1 ) and lymphocyte function associated antigen 1 ( LFA 1 ) in experimental autoimmune thyroiditis , induced by immunizing rats with thyroglobulin in complete Freund ' s adjuvant . ^^^ The antibody against LFA 1 , but not against ICAM 1 , reduced thyroglobulin antibody production ( P < 0 . 01 ) and both antibodies caused a significant reduction ( P < 0 . 002 ) in the severity of the thyroidal lymphocytic infiltration . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Blocking of neuro 2a / LN and neuro 2a / ICAM 1+ lysis with anti ICAM 1 monoclonal antibodies ( mAbs ) did not account for all the LFA 1 dependent killing . ^^^ ICAM 2 accounted for the majority of the LFA 1 dependent killing in the ICAM 1 negative target , neuro 2a / LN , while ICAM 1 played a primary role in the cytolysis of the ICAM 1+ transfectant . ^^^ Inhibition of lysis in the presence of anti ICAM 1 and ICAM 2 mAbs was comparable to that seen with the addition of anti LFA 1 mAb , indicating that other LFA 1 ligands were not involved in this system . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In parallel , an induction in the expression of the adhesion molecules ICAM 1 and LFA 1 was found , and blocking of these molecules by antibodies abolished the effect . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Endothelial and intimal cells express ICAM 1 , leukocytes LFA 1 : Endothelium expresses class 1 , but not class 2 , antigens . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Fourth , all T cell lines tested show an inability to adhere to purified ICAM 1 via LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Blocking experiments confirmed the importance of ICAM 1 on PCI and lymphocyte function associated antigen 1 ( LFA 1 ) on LAK cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Increased expression of adhesion molecules ( ICAM 1 and LFA 1 ) on alveolar macrophages from asthmatic patients . ^^^ We studied the expression of two adhesion molecules ( ICAM 1 and LFA 1 ) on alveolar macrophages recovered by bronchoalveolar lavage from 11 normal subjects and 13 asthmatic patients by using immunocytochemistry . ^^^ The percentage of cells expressing ICAM 1 or LFA 1 was significantly increased in asthmatic patients , as compared with normal subjects ( P < 0 . 001 and P < 0 . 002 , respectively ; Mann Whitney U test ) , and there was a significant correlation with the percentage of cells expressing both markers in asthma ( P < 0 . 03 , Spearman rank test ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The monoclonal antibodies against CD11a , CD11b , CD 18 , and ICAM 1 showed almost complete inhibition of the increase in intracellular peroxide levels of the endothelial cells exposed to PMA stimulated leukocytes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Undifferentiated RN33B cells express class 1 but not class 2 antigens of the major histocompatibility complex ( MHC ) , and intercellular adhesion molecule 1 ( ICAM 1 ) , a ligand for lymphocyte function associated antigen 1 ( LFA 1 ) , expressed on cytotoxic T lymphocytes ( CTLs ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Activation of cells bearing LFA 1 increased adhesiveness for ICAM 3 in parallel to adhesiveness for ICAM 1 . ^^^ Two ICAM 3 mAbs , CBR IC3 / 1 and CBR IC3 / 2 , were required to block LFA 1 dependent adhesion to purified ICAM 3 or LFA 1 dependent , ICAM 1 , ICAM 2 independent homotypic adhesion of lymphoid cell lines . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Pre treatment of A 431 , HT 29 and MDA MB 468 with anti ICAM 1 MAb inhibited cytolysis , further supporting the functional involvement of the LFA 1 / ICAM adhesion pathway in biMAb targeted tumor cell lysis . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
ICAM 1 independent , CD 18 dependent adhesion between neutrophils and human airway epithelial cells exposed in vitro to ozone . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
A role for nonspecific ( cyclosporin A ) or specific ( monoclonal antibodies to ICAM 1 , LFA 1 , and IL 10 ) immunomodulation in the prolongation of skin allografts after antigen specific pretransplant immunization or transfusion . ^^^ When mice were given pretransplant transfusion via the lateral tail vein and a combination of anti LFA 1 and anti ICAM 1 mAbs , increased graft survival was again seen . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
LAL treated peripheral blood leukocytes ( PBL ) adhered strongly to IL 1 beta activated human umbilical vein endothelial cells ( HUVEC ) , and the interaction could be inhibited by antibodies to intercellular adhesion molecule ( ICAM 1 ) or lymphocyte function associated antigen 1 ( LFA 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The cryostat sections were stained with adenosine triphosphatase as well as with the indirect immunoperoxidase technique employing murine monoclonal antibodies to HLA DR , ICAM 1 , CD 1 , CD11a , and CD 18 . ^^^ RESULTS HLA DR and ICAM 1 expression by epidermal cells , combined with perivascular accumulation of mononuclear cells with CD11a and CD 18 expression , was observed in all sites injected with IFN gamma . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To elucidate the functional roles of the intermediate alpha beta TcR cells in host defence against bacterial infection , we examined the effects of depletion of the intermediate alpha beta TcR cells by in vivo administration of monoclonal antibodies ( mAb ) to intercellular adhesion molecule 1 ( ICAM 1 ) / LFA 1 and alpha beta TcR on the bacterial growth in the liver after infection with Salmonella chorelaesuis in mice . ^^^ Pretreatment with mAb to LFA 1 ( 200 micrograms / mouse ) together with mAb to ICAM 1 ( 200 micrograms / mouse ) , which could preferentially deplete the intermediate alpha beta TcR cells and gamma delta TcR cells in the liver , resulted in a severely reduced ability to resolve acute phase of Salmonella infection in the liver . ^^^ These results suggest that intermediate alpha beta TcR cells may play an important role in protection at the early stage after Salmonella infection in liver and that the interaction of ICAM 1 / LFA 1 is critically involved in protective roles of extrathymic T cells bearing intermediate alpha beta TcR in liver at the early stage after Salmonella infection . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Biopsy specimens taken from skin before , during , and after cyclosporin treatment were stained immunohistochemically for CD 54 ( ICAM 1 ) , CD 29 ( beta 1 integrins ) , and CD 18 ( beta 2 integrins ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Antinephritis effect of anti ICAM 1 monoclonal antibody and anti LFA 1 monoclonal antibody on accelerated type anti GBM nephritis in rats ] . ^^^ Therefore we examined the effect of ICAM 1 and lymphocyte function associated antigen 1 ( LFA 1 ) in the pathogenesis of this nephritis . ^^^ These results suggested that the ICAM 1 / LFA 1 pathway is critically involved in the pathogenesis of accelerated type anti GBM nephritis in rats . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We recently reported that cross linking the leukocyte common antigen ( CD 45 ) can rapidly induce aggregation of human peripheral blood mononuclear cells via lymphocyte function associated antigen 1 ( LFA 1 ) and intercellular adhesion molecule 1 ( ICAM 1 ) interactions . ^^^ We conclude that cross linking the leukocyte common antigen on T cells induces LFA 1 / ICAM 1 dependent T cell monocyte aggregation through a unique signaling pathway independent of PKC , which involves instead cAMP / cGMP dependent protein kinases . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We have examined whether shedding intercellular adhesion molecule 1 ( ICAM 1 ) antigen from cultured tumors is able to inhibit the leukocyte function associated antigen 1 ( LFA 1 ) / ICAM 1 interaction between cytotoxic effector cells and ICAM 1+ target tumor cells . ^^^ These findings suggest that shedding of ICAM 1 antigen could be involved in binding of LFA 1 to LAK cells , resulting in reduced cytolytic activity . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The chicken beta 2 can associate on the cell surface with the human alpha subunit of LFA 1 and yields a hybrid molecule capable of binding to purified ICAM 1 and ICAM 3 . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The role of intercellular adhesion molecule 1 ( ICAM 1 ) and its ligand , leukocyte function associated antigen 1 ( LFA 1 ) , in the interaction between bladder cancer cells and lymphokine activated killer ( LAK ) cells was investigated . ^^^ Monoclonal antibodies to ICAM 1 and LFA 1 significantly decreased the binding of LAK cells to the cell lines that express ICAM 1 ( 37 to 75 % reduction , p < 0 . 05 ) , and cytokine treatment ( IFN gamma , TNF alpha ) of these cells enhanced ICAM 1 dependent adherence ( 18 to 39 % increase , p < 0 . 05 ) . ^^^ Monoclonal antibodies to LFA 1 decreased LAK cell mediated cytolysis of the bladder cancer cells from 27 to 65 % ( p < 0 . 05 ) , but anti ICAM 1 antibodies were much less effective ( 0 to 25 % decrease in cytolysis ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Suppression of experimental uveitis with monoclonal antibodies to ICAM 1 and LFA 1 . ^^^ Intercellular adhesion molecule 1 ( ICAM 1 ) , which is one of the ligands for lymphocyte function associated antigen 1 ( LFA 1 ) , plays an important role in immune responses . ^^^ To examine whether ICAM 1 and LFA 1 are involved in the pathogenesis of experimental autoimmune uveoretinitis ( EAU ) , the authors investigated the therapeutic effect of anti ICAM 1 monoclonal antibody ( mAb ) 1A29 and anti LFA 1 alpha chain mAb WT . 1 on retinal soluble antigen ( S Ag ) and Freund ' s complete adjuvant ( FCA ) induced EAU in rats . ^^^ After immunization with S Ag and FCA , rats were intraperitoneally injected with a monoclonal antibody , anti ICAM 1 mAb 1A29 or anti LFA 1 alpha chain mAb or control Ab , at 1 . 0 mg / kg body weight , according to the treatment schedule . ^^^ These findings indicate that ICAM 1 , LFA 1 dependent pathways are involved in the pathogenesis of EAU . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Lymphocyte function associated antigen 1 ( LFA 1 ) is a beta 2 integrin that participates in a broad range of leukocyte functions through binding to its ligand intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^ The location of the ICAM 1 binding site on LFA 1 is not known . ^^^ In this report , we show that the LFA 1 1 domain contains a binding site for ICAM 1 , which can be expressed as an isolated functional unit . ^^^ The 1 domain contains the epitopes for 18 out of 20 anti LFA 1 monoclonal antibodies , many of which interfere with the interaction between LFA 1 and ICAM 1 . ^^^ The 1 domain binds directly to purified recombinant ICAM 1 and also inhibits LFA 1 dependent T cell adhesion to ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We have examined the expression of intercellular adhesion molecule 1 ( ICAM 1 ) and lymphocyte function associated antigen 1 ( LFA 1 ) using monoclonal antibodies and immunohistochemistry in very early murine cGVHD . ^^^ Adherence of leukocytes to ICAM 1 positive cells is mediated by LFA 1 and this binding is thought to play an important role in a number of cell adhesion events in immune reactions . ^^^ By Day 14 , there are increasing numbers of ICAM 1 expressing cells and increased epidermal reactivity for ICAM 1 ; and finally , an increased number of LFA 1 positive infiltrating MNC . ^^^ These results support the concept that ICAM 1 / LFA 1 interactions play a role in the immune regulation of cGVHD . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The increased adhesion was inhibited by monoclonal antibodies directed against the beta 2 integrin CD 18 and endothelial cell adhesion molecule ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Cytolysis of the sensitive cells was inhibited by monoclonal antibodies against the surface proteins LFA 1 and ICAM 1 ; however , surface expression of ICAM 1 was similar on the resistant and sensitive cell lines . ^^^ Prevention of post translational addition of N linked oligosaccharides by treatment of the resistant cells with tunicamycin resulted in a dramatic enhancement in LAK cell cytotoxicity which was partially inhibited by antibodies against LFA 1 and ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Integrin LFA 1 alpha subunit contains an ICAM 1 binding site in domains 5 and 6 . ^^^ In order to identify a binding site for ligand intercellular adhesion molecule 1 ( ICAM 1 ) on the beta 2 integrin lymphocyte function associated antigen 1 ( LFA 1 ) , protein fragments of LFA 1 were made by in vitro translation of a series of constructs which featured domain sized deletions starting from the N terminus of the alpha subunit of LFA 1 . ^^^ The two regions critical for the interaction of LFA 1 with ICAM 1 lie adjacent to each other , the first next to the non functional EF hand in domain 5 and the second coinciding with the potential divalent cation binding loop in domain 6 . ^^^ In short , a critical motif for ICAM 1 binding to the alpha subunit of LFA 1 is shared between two regions of domains 5 and VI . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Autoreactive T cell proliferation was suppressed by monoclonal antibodies directed against ICAM 1 or lymphocyte function antigen 1 ( LFA 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The expression of intercellular adhesion molecule 1 ( ICAM 1 ) and lymphocyte function associated antigen 1 ( LFA 1 ) in the livers of 11 patients with chronic hepatitis B was studied immunohistochemically by light and electron microscopy to clarify the role of these adhesion molecules in tissue damage in chronic hepatitis B . ^^^ In contrast , when the liver inflammation was severe , ICAM 1 was distributed on the entire surface of the hepatocyte , including the sinusoidal and lateral membranes ; lymphocytes which were mostly positive for LFA 1 , were often observed invading deeply among these hepatocytes . ^^^ These results suggest that the ICAM 1 / LFA 1 pathway is involved in the immunological mechanism responsible for liver cell damage in chronic hepatitis B . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
T cells were also activated , as indicated by the induction of IL 2 receptor ( IL 2R ) and the enhanced expression of leucocyte function associated molecule 1 ( LFA 1 ) and ICAM 1 , a pair of adhesion molecules which have also been regarded as T cell activation markers . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Cell surface distribution of high avidity LFA 1 detected by soluble ICAM 1 coated microspheres . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In the current study we used immunogold ultrastructural techniques to identify the exact sites of expression of ICAM 1 ( CD 54 ) in the glomerulus and the expression of CD11a and CD 18 by infiltrating glomerular leukocytes in the first 24 hours of accelerated anti GBM disease in rats . ^^^ The ICAM 1 / LFA 1 interaction in glomerular leukocytic accumulation in anti GBM glomerulonephritis . ^^^ In contrast PMNL expressed only very sparse surface CD11a , suggesting that another beta 2 integrin , Mac 1 , which shares a common beta chain with LFA 1 may be the more important PMNL counter receptor for ICAM 1 in the glomerulus . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
SZ induced insulitis and blood glucose concentrations were reduced by treatment with anti intercellular adhesion molecule 1 ( ICAM 1 ) and anti lymphocyte function associated antigen 1 ( LFA 1 ) monoclonal antibodies . ^^^ These results suggest that the expression of ICAM 1 in islets and LFA 1 on mononuclear cells may be important in the development of SZ induced insulitis . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Lymphocyte migration into the CNS modelled in vitro : roles of LFA 1 , ICAM 1 and VLA 4 . ^^^ The ligand for LFA 1 could not be determined with certainty , but if it is ICAM 1 , the levels of ICAM 1 on brain endothelium are not critical . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
TAM2D2 cells did not adhere to surfaces coated with the LFA 1 counterstructure ICAM 1 , but Mn2+ induced adhesion . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule 1 ( ICAM 1 ) is one of three immunoglobulin superfamily members that bind to the integrins lymphocyte function associated 1 ( LFA 1 ) and Mac 1 on leukocytes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Furthermore , the effect of nedocromil was evaluated on the surface expression of the following markers expressed by PMNCs upon activation : ICAM 1 ( CD 54 ) , LFA 1 and alpha 1 acid glycoprotein ( alpha 1 AGP ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The role of the adhesion glycoproteins CD 18 and intercellular adhesion molecule 1 ( ICAM 1 ) in inflammatory responses produced during a reversed passive Arthus ( RPA ) reaction and induced by zymosan and zymosan activated plasma ( ZAP ) were studied in rabbit skin . 2 . ^^^ We conclude that oedema formation and haemorrhage associated with RPA reactions and in responses to zymosan and ZAP are completely CD 18 dependent , and are mediated , at least in part , via ICAM 1 . ^^^ Responses to the neutrophil independent oedema forming mediators , PAF and BK are not dependent upon CD 18 or ICAM 1 . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In contrast , addition of anti LFA 1 beta ( CD 18 ) mAb , directed against the T cell ligand of ICAM 1 , inhibited T cell proliferation almost completely . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The NG2B12 induced homotypic adhesion is mediated by LFA 1 . mAb against ICAM 1 completely inhibited the induced adhesion of activated cells but inhibited only partially and in a time dependent manner the adhesion of resting thymocytes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
These slides were also stained for ICAM 1 and for leucocyte function associated antigen 1 ( LFA 1 ) . ^^^ Many neutrophils expressing LFA 1 and some lymphocytes were also noted in the cytospin slides and some of these were conjugated to tumour cells expressing ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Increased ICAM 1 and LFA 1 expression with developing rejection following rat small bowel transplantation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Pulmonary expression of ICAM 1 and LFA 1 in experimental Goodpasture ' s syndrome . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To determine the role of cell adhesion molecules , we evaluated the in vivo effects of anti intercellular adhesion molecule 1 ( ICAM 1 ) monoclonal antibody ( 1A29 ) and anti leukocyte function associated molecule 1 ( LFA 1 ) monoclonal antibody ( WT 1 ) on ovalbumin ( OA ) sensitized rats . ^^^ These results suggest some pathophysiological roles of ICAM 1 and LFA 1 in nasal allergy . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In our previous work , we demonstrated that the presence of ICAM 1 on the surface of cardiac myocytes is required for neutrophils to directly damage them ; blocking monoclonal antibodies to either ICAM 1 on cardiac myocytes or Mac 1 on activated neutrophils completely precluded neutrophil induced myocyte injury . ^^^ We also demonstrated that postischemic cardiac lymph ( cardiac extracellular fluid ) contained leukotactic factors ( primarily C5a ) and cytokines present in concentrations sufficient to maximally induce Mac 1 on the surface of neutrophils and ICAM 1 on the surface of isolated dog cardiac myocytes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
MHC class 2 molecules , but lack the capacity to bind to T cells and do not express the crucial receptor / counter receptor lymphocyte function associated antigen 1 ( LFA 1 ) / intracellular adhesion molecule 1 ( ICAM 1 ) pair , also induced non allospecific T cell proliferative hyporesponsiveness ; this was not due to any direct inhibitory effect on the T cells . ^^^ Secondly , monoclonal antibodies ( mAb ) directed to LFA 1 and ICAM 1 were used to disrupt T cell APC adhesion specifically , while allowing for T cell receptor MHC interaction to occur . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibody ( mAb ) blocking experiments and simultaneous determination of MHC class 1 , class 2 , lymphocyte function associated antigen 1 ( LFA 1 ) and intracellular adhesion molecule 1 ( ICAM 1 ) density by quantitative radioimmunometric methods and flow cytometry on target cells demonstrated that lysis was restricted by MHC class 1 and dependent upon the constitutive MHC class 1 antigen expression . ^^^ None of the cytokines tested in this analysis , however , increased the expression of MHC class 1 , class 2 , ICAM 1 and LFA 1 on PBMC . ^^^ The mechanism of cytokine induced enhanced lytic susceptibility to TCL was not explained by increased MHC class 1 , LFA 1 or ICAM 1 expression , since no correlation was found between surface expression of these molecules and lytic susceptibility to TCL . ^^^ These data demonstrate that : ( 1 ) the constitutive density of MHC class 1 antigens determines the extent of TCL lysis ; ( 2 ) IFN gamma , and not IFN alpha or TNF alpha controls the amount of K target cell lysis by increasing their MHC class 1 antigen expression ; and ( 3 ) IFN gamma and IFN alpha control the amount of PBMC target cell lysis by a mechanism independent of MHC class 1 , ICAM 1 or LFA 1 expression . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Role of LFA 1 / ICAM 1 in the activation of murine thymocytes stimulated by Con A ] . ^^^ The biological significance of the signals triggered by the interaction of cell surface expressed LFA 1 and ICAM 1 has been investigated in Con A stimulated murine thymocytes . ^^^ Addition of anti LFA 1 or / and anti ICAM 1 McAb to the thymocyte culture in the presence of Con A , the cell proliferation was profoundly inhibited . ^^^ Such inhibitory effect was more significant by the addition of anti LFA 1 than by the addition of anti ICAM 1 McAb . ^^^ Furthermore , neither anti LFA 1 nor anti ICAM 1 McAb could block PMA+A23187 induced thymocyte proliferation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Effect of LFA 1 and ICAM 1 antibody treatment on murine corneal allograft survival . ^^^ To examine the effect of anti LFA 1 and anti ICAM 1 antibody treatment on orthotopic corneal graft survival in a mouse model . ^^^ Anti LFA 1 and anti ICAM 1 antibodies were administered intraperitoneally before and shortly after orthotopic corneal transplantation . ^^^ Both CTL and DTH responses to donor alloantigens were severely depressed in hosts treated with either anti LFA 1 or anti ICAM 1 antibody . ^^^ However , neither anti ICAM 1 nor anti LFA 1 antibody treatment prevented the rejection of orthotopic corneal grafts in previously immunized mice . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
However , the mean fluorescence associated with ICAM 1 on monocytes and CD11a and CD 18 expression on lymphocytes was related to clinical stage of disease . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
LFA 1 alpha ( lymphocyte function associated antigen 1 alpha ) positive cells were also significantly more numerous in the chronic phase than in the early one , consistent with the expression of HLA DR antigens and ICAM 1 on keratinocytes mentioned above . ^^^ Third , the LFA 1 / ICAM 1 pathway may play an important role with regard to cell adhesion of the infiltrating cells in the psoriatic lesions in disease exacerbation or prolongation , whereas the VLA 4 / VCAM 1 ( vascular cell adhesion molecule 1 ) pathway may be more important in disease onset . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Characterization of CTLL 2 Lck cells revealed markedly elevated levels of both the alpha ( CD11a ) and beta ( CD 18 ) chains of the cell adhesion molecule lymphocyte function associated 1 ( LFA 1 ) and increased binding of these T cells to a recombinant protein representing the extracellular domain of the LFA ligand , intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Prolongation of hepatic allograft survival with antibodies to ICAM 1 and LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Prevention of autoimmune diabetes by treatment with anti LFA 1 and anti ICAM 1 monoclonal antibodies . ^^^ One such pair of molecules thought to be of importance in IDDM is lymphocyte function related antigen 1 ( LFA 1 ) and intercellular adhesion molecule 1 ( ICAM 1 ) because culture of islet cells with cytokines results in the expression of ICAM 1 on islet cells in vitro . ^^^ To understand the importance of this interaction in the development of autoimmune diabetes , we have studied the ability of monoclonal antibodies ( mAbs ) against LFA 1 and / or ICAM 1 to prevent disease in multidose streptozotocin induced diabetes mellitus ( MDSDM ) . ^^^ Treatment with both anti LFA 1 and anti ICAM 1 mAbs reduced the development of hyperglycemia and insulitis in this model . ^^^ Treatment with the anti LFA 1 and anti ICAM 1 mAbs caused modulation of LFA 1 and ICAM 1 expression on splenocytes , respectively . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule 1 ( ICAM 1 ) is the natural ligand of the T lymphocyte adhesion molecule LFA 1 ( lymphocyte function associated antigen 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
This process is inhibited by antibodies to CD 18 and ICAM 1 . 5 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Prevention of autoimmune insulin dependent diabetes in non obese diabetic mice by anti LFA 1 and anti ICAM 1 mAb . ^^^ Here we present several lines of evidence suggesting that leukocyte function associated antigen 1 ( LFA 1 ) and its counter receptor intercellular adhesion molecules ( ICAM 1 ) , one of the most important pairs among these adhesion molecules , are involved in the development of autoimmune diabetes in the non obese diabetic ( NOD ) mouse . ^^^ In vivo administration of anti LFA 1 or anti ICAM 1 mAb from 5 to 30 ( or 12 ) weeks of age exerted a very strong preventative effect on the development of spontaneous diabetes with a marked reduction of insulitis , whereas both antibodies , even combined to use simultaneously , could not prevent cyclophosphamide induced diabetes . ^^^ The present study , therefore , provides the first evidence that immunointervention to LFA 1 ICAM 1 interaction has a strong prophylactic effect on autoimmune diabetes in NOD mice . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Peritoneal mast cells also were found to express the accessory molecules ICAM 1 ( CD 54 ) and LFA 1 beta ( CD 18 ) but not LFA 1 alpha ( CD11a ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Distinct binding of T lymphocytes to ICAM 1 , 2 or 3 upon activation of LFA 1 . ^^^ LFA 1 ( CD11a / CD18 ) mediates leukocyte adhesion by binding to one of its ligands : ICAM 1 , ICAM 2 or ICAM 3 . ^^^ Here , we investigated whether stimuli known to induce adhesion to ICAM 1 were also capable of inducing LFA 1 mediated adhesion of T lymphocytes to ICAM 2 and 3 transfectants . ^^^ We observed that phorbol 12 myristate 13 acetate , Mn2+ , cross linking of CD 3 or activating antibodies against LFA 1 enhanced LFA 1 mediated T cell adhesion to ICAM 2 and 3 , although to a lesser extent than to ICAM 1 . ^^^ These results indicate that , similar to what has been reported for adhesion to ICAM 1 , activation of LFA 1 is also required for adhesion to ICAM 2 and 3 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
These results indicate that the interaction between LFA 1 and ICAM is a critical step in HIV mediated syncytia formation , and that ICAM 1 , ICAM 2 and ICAM 3 are the receptor molecules for the LFA 1 dependent syncytia formation . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
On AM from normal subjects , the effects of histamine were studied on the expression of adhesion molecules ( LFA 1 , ICAM 1 ) and CD23b as well as on the release of fibronectin . ^^^ The expression of LFA 1 , ICAM 1 , and CD23b was examined by immunocytochemistry using the alkaline phosphatase anti alkaline phosphatase technique . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
These results confirm that leukocyte adhesion molecules can promote gp 160 mediated syncytium formation and demonstrate , for the first time , that adhesive interactions mediated by ICAM 1 and its contrareceptor , LFA 1 , attenuate the syncytium inhibiting activity of virus neutralizing monoclonal antibodies and soluble CD 4 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Expression of LFA 1 alpha and LFA 1 beta was normal on resting cord T cells ; however , they expressed significantly less ICAM 1 ( CD 54 ) than did adult PBMC . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In vivo treatment with anti ICAM 1 and anti LFA 1 antibodies inhibits contact sensitization induced migration of epidermal Langerhans cells to regional lymph nodes . ^^^ Since ICAM 1 and LFA 1 play important roles in leukocyte migration , we sought to determine whether in vivo administration of anti ICAM 1 and anti LFA 1 antibodies would inhibit contact sensitization induced migration of epidermal Langerhans cells to regional lymph nodes . ^^^ Dendritic lymph node cells from mice treated with anti ICAM 1 and anti LFA 1 mAb were devoid of brightly FITC stained cells and cells capable of stimulating the FITC specific T cell hybridoma . ^^^ The combination of anti ICAM 1 and anti LFA 1 mAb completely inhibited the induction of contact hypersensitivity to FITC . ^^^ Animals treated with anti ICAM 1 or anti LFA 1 monoclonal antibodies alone had significantly reduced ( by 79 and 36 % , respectively ) numbers of brightly stained cells capable of stimulating the hybridomas . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The Fc gamma RII ( CD 32 ) , the MHC class 2 antigen HLA DR and the adhesion molecules CD11a ( LFA 1 alpha ) , CD 18 and CD 54 ( ICAM 1 ) showed an unimodal distribution . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In contrast to controls , the retrobulbar adipose tissue showed an increase of HLA DR expression , an activation of intercellular adhesion molecule 1 ( ICAM 1 , CD 54 ) , as well as a marked infiltration of activated T cells ( especially CD 4 ) and macrophages ( CD 68 ) , whereas a positive staining for interleukin 2 receptors ( CD 25 ) , T cells ( CD 2 ) , B cells ( CD19 / 22 ) , complement factors ( C3b ) , and LFA 1 ( CD11a ) was present only in a few patients ' specimens . ^^^ The perimuscular connective tissue sections of GO patients showed an accumulation of T cells ( CD4 / CD8 ) , an enhanced display of ICAM 1 , LFA 1 , HLA DR positive cells , and B cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In this study we investigated the presence of lymphocyte function associated antigen 1 ( LFA 1 ) and intercellular adhesion molecule 1 ( ICAM 1 ) on renal cell cancer in 16 patients who underwent nephrectomy . ^^^ Our results suggest that the expression of ICAM 1 and LFA 1 on the surface of malignant cells may explain the unusual clinical aspects of renal cell carcinoma , its association with the immune system , and clarify certain aspects of immunotherapy . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Expression of leukocyte function associated antigen 1 ( LFA 1 ) and cluster of differentiation 44 ( CD 44 ) was observed mainly on T cells and macrophages and expression of intercellular adhesion molecules 1 ( ICAM 1 ) was observed on sinusoidal endothelial cells ( SEC ) . ^^^ Expression of LFA 1 and CD 44 was mainly observed on macrophages and expression of ICAM 1 was found on SEC . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The TGF beta induced cell adhesion phenomena are not dependent on LFA 1 , intercellular adhesion molecule 1 ( ICAM 1 ) , very late Ag 4 ( VLA 4 ) , or very late Ag 5 ( VLA 5 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Interleukin 4 and human immunodeficiency virus stimulate LFA 1 ICAM 1 mediated aggregation of monocytes and subsequent giant cell formation . ^^^ Monocyte expression of ICAM 1 but not LFA 1 was significantly enhanced by IL 4 although substrate adherence was a more potent stimulus . ^^^ Incubation with anti LFA 1 ( anti CD11a , anti CD 18 ) and anti ICAM 1 ( anti CD 54 ) monoclonal antibodies reduced IL 4 stimulated aggregation in HIV infected and uninfected monocytes and subsequently reduced MGC formation . ^^^ Hence IL 4 markedly enhanced monocyte aggregation in both HIV infected and uninfected monocytes , probably through enhanced LFA 1 ICAM 1 interactions in all cultures and LFA 1 ICAM 2 interactions in infected monocytes , leading subsequently to MGC formation in some cultures . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
CD14+ macrophages from MM , MGUS and normal donor BM are CD11a+ ( alpha L ) , CD11b+ ( alpha M ) , CD11c+ ( alpha 10 ) , CD54+ ( ICAM 1 ) , CD56+ ( N CAM ) , beta 1 and beta 2 ( CD 18 ) integrin positive . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
For instance , several studies have shown that in vivo anti LFA 1 plus anti ICAM 1 mAb treatment results in prolongation of allograft survival . ^^^ Only mAbs specific for the LFA 1 binding epitope of ICAM 1 were found to inhibit a mixed islet / lymphocyte reaction in vitro and block graft rejection in vivo . ^^^ These results suggest that the LFA 1 / ICAM 1 interaction is a critical component for xenograft rejection and , more important , that pretreatment of islet tissue with anti adhesion molecule antibodies can profoundly alter graft recognition and rejection in the absence of any systemic drug therapy . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Role of Mac 1 and ICAM 1 in ischemia reperfusion injury in a microcirculation model of BALB / C mice . ^^^ The leukocyte beta 2 integrin Mac 1 ( CD11b / CD18 ) and its endothelial ligand intercellular adhesion molecule 1 ( ICAM 1 ) are involved in leukocyte adhesion to and macromolecular leakage from postcapillary venules during inflammatory reactions . ^^^ Using intravital fluorescence microscopy and a microcirculation model in awake BALB / C mice , we investigated the effects of monoclonal antibodies ( MAb ) and Fab fragments to Mac 1 and MAb to ICAM 1 on leukocyte endothelium interaction and macromolecular leakage of fluorescein isothiocyanate dextran ( 1 . 5 10 10 ( 5 ) mol wt ) in striated skin muscle after 3 h of ischemia followed by reperfusion . ^^^ We demonstrated that administration of MAb and Fab to Mac 1 before reperfusion was as effective as administration of MAb to ICAM 1 , which was found to be significantly upregulated in the postischemic tissue by immunohistochemical analysis , in preventing postischemic leukocyte adhesion to and macromolecular leakage from postcapillary venules , whereas postischemic leukocyte rolling was not affected after MAb administration . ^^^ Postischemic capillary perfusion was efficiently preserved in animals treated with anti Mac 1 and anti ICAM 1 MAb compared with animals receiving the isotype matched control antibodies . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Thus there may be a pathogenetic role for ICAM 1 expression and for intraglomerular accumulation of leucocytes , especially PMNs , which express LFA 1 in the development of GN . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To investigate the significance of intercellular adhesion molecule 1 ( ICAM 1 ) and leukocyte function associated antigen 1 ( LFA 1 ) in host defense against infection with intracellular parasites , we examined the effects of in vivo pretreatment with mAbs to ICAM 1 ( 1A29 ) and LFA 1 alpha ( WT 1 ) on the protection against infection with Listeria monocytogenes in Fisher F344 / N rats . ^^^ Expression of ICAM 1 and LFA 1 alpha molecules on T cells in spleen , liver and peritoneal cavity of rats was down regulated after i . p . administration with daily doses of 300 micrograms of either 1A29 or WT 1 for 10 days . ^^^ Thus , the resistance against listerial infection was severely impaired by combinational pretreatment with mAbs in ICAM 1 and LFA 1 alpha . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Indirect immunoperoxidase staining patterns indicated that intratracheal challenge with beads activated BAL cells and upregulated expression of CD11b , ICAM 1 , and CD 18 antigenic markers on macrophages and neutrophils when compared with BAL cells from saline controls . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Expression of adhesion molecules in transplanted lung allografts immunohistochemical study using the rat lung transplantation model ] . 1 have carried out orthotopic left lung transplantation in rats in order to investigate role of adhesion molecules and the time course of lung allograft rejection . 1 used an RT 1 incompatible rat lung allograft model and examined the time course of organ rejection and the expression of ICAM 1 and LFA 1 molecules in two combinations of inbred rat strains , WKAH / Hkm and F344 / NS1c . ^^^ ICAM 1 detectable in the normal lung , was also detected on the pneumocyte cell surface at all stages of acute rejection , suggesting that pneumocytes were target cells for LFA 1 positive cells . ^^^ These data suggested that ICAM 1 and LFA 1 participate in the course of acute lung allograft rejection . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To examine the role of adhesion molecules in T cell recruitment and activation during allergen induced late asthmatic response ( LAR ) , we evaluated the expression of lymphocyte function associated antigen 1 alpha ( LFA 1 alpha ) and intercellular adhesion molecule 1 ( ICAM 1 ) on peripheral blood T lymphocyte subsets from atopic asthmatic patients and their changes following allergen inhalation challenge . 12 atopic asthmatic patients were studied . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule 1 ( ICAM 1 ) binds circulating leukocytes through interactions with beta 2 integrins , LFA 1 , and macrophage Ag 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Whether antibody blockage of leukocyte ligands , CD 18 , for intercellular adhesion molecule ( ICAM 1 ) would prevent allograft rejection was studied in a rabbit heterotopic transplant model . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The expression of intercellular cell adhesion molecule 1 ( ICAM 1 ) , LFA 1 and HLA DR was investigated by immunohistochemistry . ^^^ The effect of anti ICAM 1 , anti LFA 1 and anti HLA DR monoclonal antibodies on IFN gamma induced MGC generation was also examined . ^^^ Finally , anti LFA 1 and anti ICAM 1 monoclonal antibodies variably inhibited IFN gamma induced MGC generation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Neutralizing antibodies against cell adhesion molecules ( ICAM 1 and LFA 1 ) regulate neutrophil : endothel adhesion and monoclonal antibodies against these adhesion molecules reduced the size of infarction . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Furthermore , ICAM 1 , which is the ligand for LFA 1 molecules , was also expressed on eosinophils with hypereosinophilia . ^^^ To extend our understanding of high intensity expression of adhesion molecules ( CR 3 , LFA 1 alpha , LFA 1 beta , ICAM 1 ) on hypodense eosinophils , we examined whether chemotactic agents or eosinophilopoietic growth lymphokines ( IL 3 , GM CSF , IL 5 ) are involved in adhesion molecule expression using an eosinophilic cell line , EoL 3 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
SEB induced proliferation of T cells was inhibited by monoclonal antibodies to CD 2 , CD11a , CD 18 , CD 28 , CD 44 , CD 58 and ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Two ligand receptor pairs may play a role in the adhesion of cytotoxic T cells to their targets : 1 ) intercellular adhesion molecule 1 ( ICAM 1 ) and lymphocyte function associated antigen 1 ( LFA 1 ) , and 2 ) LFA 3 and CD 2 . ^^^ Autoaggressive inflammatory cells close to invaded muscle fibers showed an increased expression of ICAM 1 and LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
These principles are illustrated with two CAMs : firstly the interaction of the homotypic epithelial / myeloid adhesins CD 66 , and the endothelial adhesin , CD 31 , and secondly the heterotypic adhesins ICAM 1 , 2 and 3 , which interact with the leukocyte integrin LFA 1 . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
This adhesion was blocked by preincubation of CaKi 1 cells with monoclonal antibody against ICAM 1 or by preincubation of PBMC with either monoclonal antibody against leucocyte function associated antigen 1 alpha ( LFA 1 alpha ) , a major receptor for ICAM 1 , supernatants from treated cultures or purified sICAM 1 molecules . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
No changes in the expression of vascular addressins , VCAM 1 , E selection or ICAM 1 could be observed but the binding of monocytes could be blocked with an antibody against Mac 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The other surface markers including classical sialyl Le ( 10 ) antigen defined by SNH 3 or FH 6 and LFA 1 , VLA 4 , CD 4 , CD 25 , ICAM 1 , Leu 8 , and HLA DR did not show a significant difference regardless of skin involvement . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In vitro binding of the monocytes was inhibited by mAbs against ICAM 1 , LFA 1 , CD 18 , and MAC 1 ; MAC 1 did not influence lymphocyte binding , although the other mAbs were effective . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In multiple sclerosis and experimental allergic encephalomyelitis ( EAE ) , endothelial cells of active lesions increase expression of the adhesion molecules such as ICAM 1 , VCAM 1 and inflammatory cells including memory T cells and macrophages express high levels of adhesion molecules such as LFA 1 , VLA 4 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies to the adhesion molecule LFA 1 and its ligand ICAM 1 reduce lysis of control targets but are less effective in blocking lysis of kifunensine treated cells . ^^^ K 562 cells bind anti ICAM 1 but not anti LFA 1 , and this binding does not change after kifunensine treatment . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The cell surface expression of CD 23 , CD 21 , ICAM 1 and LFA 1 was monitored on transfected cells . ^^^ LMP 1 also induced increased CD 21 , ICAM 1 and LFA 1 surface expression on transfected primary T cells , and CD 21 and ICAM 1 in four of five B cell chronic lymphocytic leukemias tested . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The adhesion molecule ICAM 1 mediates leucocyte adhesion to target structures and other immune cells by binding with the leucocyte adhesion receptors LFA 1 and MAC 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Repeated injection of mAb against rat ICAM 1 or alpha subunit of LFA 1 ( LFA 1 alpha ) dose dependently prevented the development of proteinuria , the influx of these cells , and subsequent crescent formation in this nephrotoxic serum nephritis model . ^^^ Furthermore , simultaneous administration of anti ICAM 1 mAb and anti LFA 1 alpha mAb exerted additive protective effects against urinary protein excretion and crescentic glomerulonephritis . ^^^ These results indicate that adhesion of CD 8 positive cells and monocytes to glomerular endothelial cells through the LFA 1 / ICAM 1 pathway is crucial for the initiation and subsequent progression of nephrotoxic serum nephritis in WKY rats . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We performed this study to emphasize the difference in the expression of certain cell adhesion molecules ( CAMs ) ( ICAM 1 , ELAM 1 , LFA 1 , VLA 3 , VLA 6 ) in lesional and perilesional skin of 10 patients with psoriasis , before and after treatment with topical Calcipotriol . ^^^ We noticed reduced levels of infiltrating cells along with the expression of ICAM 1 , LFA 1 , ELAM 1 and of CAMs VLA 3 , VLA 6 in basal and suprabasal keratinocytes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We distinguish four categories of markers , which are characteristic of : ( 1 ) macrophage precursors and immature macrophages ( ER MP 12 , ER MP 20 , ER MP 54 , ER MP 58 ) ; ( 2 ) mature macrophages in general ( F4 / 80 , BM 8 , Mac 1 , Mac 2 , ER BMDM 1 ) ; ( 3 ) macrophage subsets ( ER HR 3 , ER MP 23 , ER TR 9 , Forssman antigen , MOMA 1 , MOMA 2 , Monts 4 , SER 4 ) , and ( 4 ) IFN gamma stimulated macrophages ( H 2Ia , LFA 1 , ICAM 1 , 158 . 2 , MBR 2 , TM 2 , TM 4 , and TM 5 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The three families of adhesion molecules that participate in this vital processes are the selectins , namely L selectin , E selectin and P selectin , the integrins : LFA 1 , CR 3 , p 150 . 95 and very late antigen 4 , and the immunoglobulin ( Ig ) superfamily which includes intercellular adhesion molecule 1 ( ICAM 1 ) , ICAM 2 and vascular cell adhesion molecule 1 ( VCAM 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Immunophenotypic study revealed that leukemic cells in her peripheral blood were positive for ICAM 1 ( CD 54 ) but negative for LFA 1 alpha ( CD11a ) and LFA 1 beta ( CD 18 ) , whereas infiltrating leukemic cells in the subcutaneous plasmacytoma of the left thigh were positive for LFA 1 alpha and LFA 1 beta but negative for ICAM 1 . ^^^ Discordant LFA 1 / ICAM 1 expression in a case of secondary plasma cell leukemia associated with subcutaneous plasmacytoma . ^^^ In the present case , however , the discordant expression of LFA 1 / ICAM 1 adhesion molecules may have accounted for the distinct patterns of growth and the spread of the subcutaneous plasmacytoma through homing of the LFA 1 alpha+ , LFA 1 beta+ leukemic cells to ICAM 1+ endothelial cells . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Since the lymphocytes presumably must adhere to the bile ducts to initiate a cell to cell mediated destruction , we have studied the expression of the lymphocyte function associated antigen 1 ( LFA 1 ) together with its ligand , the intercellular adhesion molecule 1 ( ICAM 1 ) , and the expression of HLA DR , using immunoperoxidase staining of cryostat sections from patients with PBC ( n = 10 ) , PSC ( n = 13 ) , and normal healthy controls ( n = 6 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To clarify the activation of peripheral blood T cells in Kawasaki disease ( KD ) patients , we investigated whether expression of lymphocyte function associated antigen 1 ( LFA 1 , CD11a / CD18 ) and / or intercellular adhesion molecule 1 ( ICAM 1 , CD 54 ) on peripheral blood T cells increases during the acute stage . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Despite higher ICAM 1 levels , cell aggregation assays revealed that LFA 1 ICAM 1 adhesive interactions were not involved in the homotypic adhesion of B9 / BM1 cells but rather that binding of CD 44 to endogenously synthesized hyaluronan was responsible . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We performed the present study to determine the effects of mouse monoclonal antibodies to the adhesion molecules intercellular adhesion molecule 1 ( ICAM 1 ) and lymphocyte function associated antigen 1 ( LFA 1 ) in a model of crescentic glomerulonephritis in Wistar Kyoto rats , induced by immunization with bovine glomerular basement membrane ( GBM ) . ^^^ The administration of monoclonal antibodies to rat ICAM 1 and LFA 1 markedly decreased the severity of the renal disease . ^^^ The kidneys of rats treated from days 2 to 14 with antibodies to ICAM 1 and LFA 1 showed bright linear staining for rat IgG along the GBM , which did not differ in intensity from that seen in untreated rats . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Prolongation in murine cardiac allograft survival with monoclonal antibodies to LFA 1 , ICAM 1 , and CD 4 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Clonal anergy induction by monoclonal antibodies to CD 4 , LFA 1 , and ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Role of ICAM 1 and LFA 1 in cardiac allograft rejection of the rat . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The interactions between T and B lymphocytes are mediated by several antigen independent adhesion molecules including LFA 1 / ICAM 1 and CD2 / LFA 3 . ^^^ This method also seems to be applicable to recombinant cells bearing a single adhesion molecule such as LFA 1 and ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Immunohistochemical staining was used to document the serial expression of Mac 1 , intercellular adhesion molecule 1 ( ICAM 1 ) , and lymphocyte function associated antigen 1 ( LFA 1 ) in eyes of C3H / HeN mice with endotoxin induced uveitis . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Factor XIIIa has previously been used to identify a subgroup of dermal dendritic cells which co express class 2 MHC antigens , intercellular adhesion molecule 1 ( ICAM 1 ) and lymphocyte function associated antigen 1 ( LFA 1 ) , leading to the suggestion that they play a central role in skin immune responses . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule 1 ( ICAM 1 ) functions as a ligand for lymphocyte function associated antigen 1 ( LFA 1 ) , and thereby plays a crucial role in mediating cell cell interactions in inflammatory reactions . ^^^ To gain more insight into the capacity of eosinophils to physically interact with LFA 1 positive inflammatory leukocytes , in the present study ICAM 1 expression in eosinophils was investigated . ^^^ In summary , these studies indicate that eosinophils may be activated to express the adhesion molecule ICAM 1 upon stimulation with selected inflammatory cytokines , which may allow adhesion mediated cross talk between eosinophils and LFA 1 positive cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The involvement of LFA 1 and ICAM 1 in CD 45 mediated adhesion was supported by the observation that CD11a ( LFA 1 alpha ) mAb R7 . 1 , CD 18 ( LFA 1 beta ) mAb R3 . 3 , and CD 54 ( ICAM 1 ) mAb R6 . 1 or RR / l all strongly inhibited CD 45 and PMA induced aggregation . ^^^ CD 45 mAb induces cell adhesion in peripheral blood mononuclear cells via lymphocyte function associated antigen 1 ( LFA 1 ) and intercellular cell adhesion molecule 1 ( ICAM 1 ) . ^^^ These results indicate that triggering human PBMCs via CD 45 can cause strong cell aggregation , largely through LFA 1 / ICAM 1 interactions . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Differential regulation of LFA 1 and ICAM 1 on human primary B lymphocytes . ^^^ To better understand the regulation of interactions between B cells and other cells during the immune response , we have studied surface expression of the adhesion molecules LFA 1 ( CD11a / CD18 ) and ICAM 1 ( CD 54 ) . ^^^ However , upon stimulation with anti IgM and IL 4 , ICAM 1 levels started to increase within 12 hr , while LFA 1 levels did not start to increase until after 36 hr . ^^^ When B cells were stimulated with the PKC activator PDB and a calcium ionophore , ICAM 1 levels , but not LFA 1 levels , increased . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
After staining with specific fluorescent monoclonal antibodies , flow cytometric analysis was performed to evaluate the expression of CD11b ( = Mac 1 , CR 3 , or C3bi receptor ) , CD11a ( = leukocyte function antigen 1 or LFA 1 , or gp 180 / 95 ) , CD 54 ( = intercellular adhesion molecule 1 or ICAM 1 ) , and CD 45 ( = leukocyte common antigen ) on circulating leukocytes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Homotypic adhesion involves mainly the interaction between the adhesion molecules Leukocyte Function Antigen 1 ( LFA 1 ) and its ligand CD 54 ( ICAM 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In addition , lysis of melanoma clones by any effector was similarly inhibited by anti ICAM 1 and anti LFA 1 antibodies . ^^^ This indicates that by pass of low lysability of ICAM 1low melanoma clones by CTL clones , after ICAM 1 up regulation , is possible only if simultaneous LFA 1 and TcR engagement takes place . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Cell cell contact and adhesion is required in immune responses , and intercellular adhesion molecule 1 ( ICAM 1 ) , which is a ligand for lymphocyte function associated antigen 1 ( LFA 1 ) , plays an important role in this process . ^^^ To investigate the essential role of the ICAM 1 / LFA 1 pathway in the cell mediated cytotoxicity involved in viral myocarditis , we examined by immunofluorescence the expression of ICAM 1 in murine hearts with acute myocarditis caused by CVB 3 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Leukocyte function associated molecule 1 ( LFA 1 [ CD11a / CD18 ] ) and intercellular adhesion molecule 1 ( ICAM 1 [ CD 54 ] ) are one such critical adhesive receptor counter receptor combination . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Adhesion molecules such as intercellular adhesion molecule 1 ( ICAM 1 ) and its counter receptor , lymphocyte function associated antigen 1 ( LFA 1 ) , play very important roles in immune responses . ^^^ Pretreatment of IFN gamma treated MMG 2 with 84H10 ( anti ICAM 1 antibody ) or pretreatment of lymphocytes with either SPV L 7 ( anti LFA 1 alpha antibody ) or IOT 10 ( anti LFA 1 beta antibody ) inhibited the lymphocyte adhesion to MMG 2 . ^^^ These results suggest that ICAM 1 molecules induced on melanoma cells by IFN gamma can interact with LFA 1 molecules on lymphocytes . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule , a ligand for the leucocyte integrins CD11a / CD18 ( LFA 1 ) and CD11b / CD18 ( Mac 1 ) , that plays an important role in a variety of inflammatory and immune mediated mechanisms , is strongly expressed in retroocular connective tissue from patients with Graves ' ophthalmopathy ( GO ) and involved in lymphocyte attachment to cultured retroocular fibroblasts via the ICAM 1 / LFA 1 mediated pathway . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
On the other hand , EoL 3 cells show constitutive expression of Fc epsilon RII , Fc gamma RII , LFA 1 and ICAM 1 on their cell surface . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
This enhancement was inhibited by a combination of CD11a , CD 18 , and ICAM 1 specific mAbs implicating a role of CD11a / CD18 in leukocyte adhesion to endothelium and diapedesis as part of the inflammatory process . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
DC express a range of adhesion molecules which are relevant to their interaction with T lymphocytes including ICAM 1 , a ligand for T lymphocyte LFA 1 . ^^^ This study details the expression of ICAM 1 and ICAM 2 , a ligand for LFA 1 , on cells of the DC lineage and examines their contribution to the DC stimulated MLR response . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Overexpression of LFA 1 and ICAM 1 in Down syndrome thymus . ^^^ The interaction between lymphocyte function associated antigen 1 ( LFA 1 ) on thymocytes and intercellular adhesion molecule 1 ( ICAM 1 ) on the thymic stroma plays an important role in thymocyte maturation . ^^^ Consequently , we examined the constitutive expression of LFA 1 and ICAM 1 in postnatal human thymus from children with Down syndrome ( trisomy 21 , DS ) and age matched control children . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies against ICAM 1 ( CD 54 ) and LFA 1 ( CD11a / CD18 ) inhibit experimental autoimmune uveitis . ^^^ We studied the expression of two cell adhesion molecules : intercellular adhesion molecule 1 ( ICAM 1 , CD 54 ) and lymphocyte function associated antigen 1 ( LFA 1 , CD11a / CD18 ) in mice with experimental autoimmune uveitis . ^^^ ICAM 1 was expressed on the vascular endothelium of the ciliary body and retina by 7 days after immunization , and LFA 1 was first expressed on some infiltrating inflammatory cells 9 days after immunization . ^^^ We then studied the effect of monoclonal antibodies against ICAM 1 and LFA 1 on the development of experimental autoimmune uveitis . ^^^ Three groups of mice were immunized and treated for 21 days with daily intraperitoneal injections of rat monoclonal antibody against murine ICAM 1 or LFA 1 or with rat IgG as control . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
ICAM 1 is also a ligand or counter receptor for the leukocyte integrin lymphocyte function associated antigen 1 ( LFA 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
There was no difference in the level of expression of CD 4 , CD 2 , or LFA 1 molecules between these Th cell clones , and anti CD 4 , CD 2 , LFA 1 or ICAM 1 mAb did not differentially affect Ag induced proliferation among the clones . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
WBC EC adherence is largely dependent on interaction between the WBC CD 18 complex and the endothelial ligand , intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^ Administration of monoclonal antibodies directed against CD 18 and / or ICAM 1 inhibit WBC EC adherence and have been reported to modulate ischemia reperfusion and inflammatory injury . ^^^ We asked the question , does inhibition of WBC EC adherence by administration of monoclonal antibody directed against either CD 18 ( R15 . 7 ) or against ICAM 1 ( R6 . 5 ) increase susceptibility to infection . ^^^ Administration of the anti CD 18 antibody , R15 . 7 , resulted in significantly increased rates of abscess formation following innoculation with S . aureus and with P . aeruginosa , compared to controls and to the animals given the antibody to ICAM 1 , R6 . 5 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies against CD11a and CD 18 inhibited the binding of lymphocytes to untreated and TNF treated HUVEC and EA . hy 926 cells and ICAM 1 expression was increased on both monolayers following treatment with TNF . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We found , that : ( 1 ) the number of TCR / CD3 receptors on uremic helper inducer ( CD 4 ) T lymphocytes is decreased and correlated well with the blunted lymphocyte proliferation induced by anti CD 3 mAb ; ( 2 ) these findings were associated with diminished binding capacity of IL 1 beta and IL 6 to their receptors ( IL 1R , IL 6R ) on helper inducer T cells , whereas ( 3 ) the IL 2 receptor ( IL 2R ) and molecule expression of CD 4 and lymphocyte function antigen 1 ( LFA 1 ) were increased , and ( 4 ) uremic monocytes displayed a decreased density of intercellular adhesion molecule 1 ( ICAM 1 ) expression , which interacts as receptor ligand pair with LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
An intimate association of T cells with macrophages , and the expression of leukocyte function associated antigen 1 ( LFA 1 ) and intercellular adhesion molecule 1 ( ICAM 1 ) , accessory molecules in antigen presentation , were observed in each cell in the region of macrophage clusters . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
HUVEC and melanoma cells did not express LFA 1 and increased ICAM 1 expression by TNF alpha , IL 1 and IFN gamma treatment in FACS analysis did not coincide with minimal increase of melanoma cells adhesion to cytokine treated HUVEC . ^^^ These results suggest that adhesion between melanoma cells and HUVEC is probably mediated by molecular interaction other than ICAM 1 / LFA 1 . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Both the aggregate formation and the proliferative effects of LZ 8 were blocked by addition of monoclonal antibody to either CD 18 or CD11a , the counterreceptor complex components for ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
This type of cell aggregation depends on the participation of LFA 1 and ICAM 1 molecules . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Dendritic cells expressed major histocompatibility ( MHC ) class 1 and class 2 molecules , the leucocyte common antigen CD 45 , the rat leucocyte antigen OX 44 , the rat macrophage marker ED 1 , and the adhesion molecules Mac 1 , LFA 1 and ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
LFA 1 and ICAM 1 in homotypic aggregation of rat alveolar macrophages : organic dust mediated aggregation by a non protein kinase C dependent pathway . ^^^ Treatment with monoclonal antibodies to lymphocyte function associated antigen 1 ( LFA 1 ) alpha and beta and intercellular adhesion molecule 1 ( ICAM 1 ) resulted in the inhibition of aggregate formation in a dose dependent manner . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The intercellular adhesion molecule 1 ( ICAM 1 ) is the ligand for the lymphocyte function associated antigen 1 ( LFA 1 ) . ^^^ The ICAM 1 / LFA 1 complex mediates cell cell and cell matrix interactions and is believed to be crucial for several immunological functions , including non MHC restricted cytotoxicity . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Antibodies to ICAM 1 , CD 18 , and HLA DR abolished the cytotoxicity , and a substantial reduction was seen with antibody to CD72 . ( ABSTRACT TRUNCATED AT 400 WORDS ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The mAbs selected were directed against intercellular adhesion molecule 1 ( ICAM 1 ) and lymphocyte function associated antigen 1 ( LFA 1 ) and were administered at different doses ( 50 micrograms 1 mg ) , as single and multiple injections , and at various time points post transfer of myelin basic protein specific lymphocytes . ^^^ This is the first report suggesting that alpha ICAM 1 and alpha LFA 1 mAbs might have opposite effects ( i . e . ameliorating or worsening ) upon murine EAE and the different effect of alpha LFA 1 might be related to this molecule being involved in more cell signalling mechanisms than ICAM 1 . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The leucocyte adhesion molecules lymphocyte functional antigen 1 ( LFA 1 ; CD11a / CD18 ) and intercellular adhesion molecule 1 ( ICAM 1 ; CD 54 ) facilitate the cell to cell interactions which are required for the initiation of immune responses . ^^^ In contrast to the well documented inhibitory effects of anti ICAM 1 or anti LFA 1 antibodies on mixed lymphocyte responses , we were unable to block these responses with the soluble form of ICAM 1 ( sICAM 1 ) . ^^^ These results suggest that antibodies which block ICAM 1 / LFA 1 not only block the adhesion which is required to stabilize cell to cell contact , but also block the costimulatory signal which is required for T cell activation . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Here we describe a novel in vitro flow cytometric adhesion assay ( FCAA ) which , based on scatter and fluorescence properties , was used to analyse the contribution of the LFA 1 / intercellular adhesion molecule 1 ( ICAM 1 ) adhesion pathway in the binding of bone marrow ( BM ) derived LFA 1 positive primary tumour cells of patients with MM to interferon gamma ( IFN gamma ) activated , ICAM 1 positive , human venous umbilical endothelial cells ( huVEC ) in vitro . ^^^ The results prove that LFA 1 expressed on bone marrow derived plasma cells from MM patients can be used for cellular adhesion to ICAM 1 expressed on adherent growing cells , and are suggestive for a role of the LFA 1 / ICAM 1 adhesion pathway in the pathophysiology of MM . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Both cells were lymphocyte function associated antigen 1 ( LFA 1 ) + / CR3 counter receptors for ICAM 1 . ^^^ However , homotypic cell adhesion was not completely inhibited by antibodies against ICAM 1 and LFA 1 , suggesting that the homotypic cell aggregation of P 39 ( + ) cells is due only in part to ICAM 1 and LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Using cell conjugation assays , we demonstrated that ICAM 1 and lymphocyte function associated antigen 1 ( LFA 1 ) interactions were important for effector to target cell conjugate formation and demonstrated that tumor cell pretreatment with rIFN gamma enhanced cell conjugate formation . ^^^ Whereas anti LFA 1 blocked all BFA mediated tumor lysis and conjugate formation , anti ICAM 1 blocked only the enhancing effects of rIFN gamma for both cytolysis and conjugate formation . ^^^ ICAM 1 , which was augmented by rIFN gamma , appears to be only one of several ligands interacting with LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Mononuclear leukocytes exhibiting strong surface LFA 1 ( CD11a and CD 18 ) expression were seen adherent to the endothelium of interstitial capillaries , with ICAM 1 and LFA 1 antigens present at sites of contact . ^^^ Recent studies of rat anti GBM disease have demonstrated a functional role of the ICAM 1 / LFA 1 interaction in the entry of leukocytes into the glomerulus and an association between interstitial ICAM 1 expression , leukocyte infiltration and tubulointerstitial damage . ^^^ In the current study , we used immunogold ultrastructural techniques to identify ICAM 1 / LFA 1 interactions in the initiation of interstitial leukocyte infiltration during the first 24 hours of rat accelerated anti GBM disease . ^^^ In addition , mononuclear cells migrating into the interstitium showed areas of close apposition to interstitial fibroblast like cells , and here ICAM 1 and LFA 1 expression were also prominent at the sites of contact . ^^^ This is the first study to demonstrate sites of ICAM 1 / LFA 1 interaction in mononuclear cell migration and localization in glomerulonephritis . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Among MoAbs to these CAMs , only Abs to CD11a / CD18 ( LFA 1 ) and CD 54 ( ICAM 1 ) decrease both target binding and cytolysis by LAK cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The VLA interaction appear to be involved in pannus attachment , whereas LFA 1 and ICAM 1 are involved in cell cell interaction and may upregulate molecules such as VLA that are involved in attachment . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
IFN induced enhanced lysis was associated with an increased binding between effectors and targets , and antibodies to ICAM 1 as well as its counter receptor LFA 1 , blocked the increased binding and lysis . ^^^ These post binding alterations , were also sensitive to blocking with anti ICAM 1 and anti LFA 1 antibodies . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies to lymphocyte function associated ( LFA 1 ) adhesive protein and to intercellular adherence molecule 1 ( ICAM 1 ) suppressed significantly the HA of Raji cells induced by VIP and PMA . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Anti CD11a ( LFA 1 ) and anti CD 54 ( ICAM 1 ) monoclonal antibodies prevented both platelet localization and hemorrhagic necrosis , and these determinants were detected on mouse platelets by immunofluorescence . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
On the basis of reports demonstrating possible roles for leukocyte function associated antigen 1 ( LFA 1 ) and intercellular adhesion molecule 1 ( ICAM 1 ) , the ligand for LFA 1 , in human immunodeficiency virus type 1 ( HIV 1 ) infection , we have explored the involvement of the ICAM 1 molecule by using selected synthetic peptides derived from the protein sequence . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Adhesion of peripheral blood mononuclear cells to lung tumour cell lines could be inhibited by monoclonal antibodies ( MAb ) ( CD11a ; CD 18 ) against the receptor of ICAM 1 , the leukocyte function associated antigen 1 ( LFA 1 ) , but not by a MAb ( CD 54 ) against ICAM 1 itself . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
No obvious correlation was found between the leukemic phenotype and the amount of MEL 14 , LFA 1 , ICAM 1 , Pgp 1 / CD44 and THAM / CD26 antigen expression . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
It was blocked by inhibitors of cellular metabolism and cytoskeletal function but not by antibodies to leucocyte function associated antigen 1 ( LFA 1 ) or intracellular adhesion molecule 1 ( ICAM 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
This syndrome may be due to an increase of production of tumor necrosis factor alpha which upregulates the endothelial expression of ICAM 1 and thus leads to adhesion of CD11a / CD18 ( LFA 1 ) bearing cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Fetal human cardiac myocytes ( FHCM ) and a cell line derived from FHCM , termed W 1 , constitutively express low levels of MHC class 1 antigens and significant levels of ICAM 1 ( CD 54 ) , and LFA 3 ( CD 58 ) but do not express LFA 1 alpha ( CD11a ) , LFA 1 beta ( CD 18 ) , GMP 140 ( CD 62 ) , BB 1 B7 , VCAM 1 , and ELAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To extend our understanding of the high intensity expression of adhesion molecules ( CR 3 , LFA 1 alpha , LFA 1 beta , ICAM 1 ) on hypodense eosinophils , which was observed in our previous study , we examined whether the supernatant of lymphocytes from mite allergic asthmatic patients is involved in adhesion molecule expression using an eosinophilic cell line ( EoL 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Leukocyte endothelial cell adhesion and shear rate were monitored in rat mesenteric venules during superfusion with either LTB 4 or PAF in the presence or absence of monoclonal antibodies ( MAbs ) directed against either leukocyte ( CD 18 , CD11b ) or endothelial cell [ intercellular adhesion molecule 1 ( ICAM 1 ) , E selectin , P selectin ] adhesion glycoproteins . ^^^ The LTB 4 and PAF induced leukocyte adherence and emigration were significantly attenuated by pretreatment with MAbs directed against CD 18 , CD11b , ICAM 1 , and E selectin , but not P selectin . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Our results indicate that infection with M . intracellulare can increase the expression of adhesion molecules , ICAM 1 and LFA 1 , only at the site of injection ( peritoneum ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Phenotypic analysis revealed in all the lines a high frequency of cells positive for CD 45 , asialo GM 1 ( ASGM 1 ) , lymphocyte function associated antigen 1 ( LFA 1 ) , intercellular adhesion molecule 1 ( ICAM 1 ) and CD 44 , but negligible expression of L selectin ( LAM 1 ) and very late antigen 4 ( VLA 4 ) ; 2 lines expressed the vitronectin receptor ( VN R ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In contrast to untreated LP lesions , in biopsies obtained after PUVA therapy from healed involved skin we found markedly diminished to completely absent expression of CD 54 ( ICAM 1 ) , HLA DR , CD11a / 18 ( LFA 1 ) and CD49f ( VLA 6 ) on keratinocytes and an effective loss of CD49a ( VLA 1 ) , CD49c ( VLA 3 ) , CD49d ( VLA 4 ) , CD49f ( VLA 6 ) , CD 3 and CD 8 positive infiltrating cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Most interestingly , the LW protein was found to exhibit sequence similarities ( with approximately 30 % identity ) with intercellular adhesion molecules ICAM 1 , 2 , and 3 , which are the counter receptors for the lymphocyte function associated antigens LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We have studied in thirty renal biopsies ( from 30 cadaver allograft patients ) the expression of both LFA 1 and VLA 4 leukocyte adhesion receptors and their respective ICAM 1 and VCAM 1 endothelial cell ligands , during early allograft dysfunction ( 24 + / 5 days after transplantation ) , reversed either by antirejection therapy ( n = 14 ) or by reduction in CsA dose ( n = 16 ) . ^^^ On the other hand , we found that the mean percentage of infiltrating cells bearing LFA 1 molecules and the intensity of ICAM 1 ( a LFA 1 ligand ) expression on endothelium were closely similar in both rejection and CsA nephrotoxicity . ^^^ Our results strongly suggest that in rejection the interstitial cell infiltrate seems to be facilitated by the contribution of both LFA 1 / ICAM 1 and VLA 4 / VCAM 1 cell adhesion mechanisms , and also that VLA 4 / VCAM 1 leukocyte interaction does not play a role in cases with CsA nephrotoxicity . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Hypoxia induced adhesion was inhibited by LFA 1 specific ( CD11a / CD18 integrin ) antibodies , but not by antibodies directed against the ICAM 1 ligand for the LFA 1 receptor . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Concurrently , we observed a reduced expression of IL 2 alpha chain receptors on surfactant treated CD56+ / CD3 cells and a dramatic reduction in the expression of adhesion molecules including CD 2 , LFA 1 , LFA 3 , and ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The second involves the lysis of these infected cells by cytotoxic effector CD4+ T cells , which is class 2 MHC restricted , requires cell to cell contact mediated by the ICAM 1 and LFA 1 ligand pair , and appears to be independent of lymphotoxins and through the direct cell interaction by adhesion molecules such as LFA 1 and ICAM 1 but not cytokines . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
However , there is no difference of staining pattern of ICAM 1 or LFA 1 antigen in any type of leprosy . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Flowcytometry demonstrated that murine endothelial cell line F 2 expresses MHC class 1 antigen , FcR 2 , Mac 1 and vascular cell adhesion molecule 1 ( VCAM 1 ) , but not intercellular adhesion molecule 1 ( ICAM 1 ) and class 2 antigen . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Treatment of transplant recipients with monoclonal antibodies against accessory molecules , such as leukocyte function associated antigen 1 ( LFA 1 ) and intercellular adhesion molecule 1 ( ICAM 1 ) , has resulted in either a prolongation of transplant survival or the induction of tolerance in some models . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The panel of adhesion molecules studied included HLA ABC , HLA DR , CD 3 , LFA 1 , MAC 1 , ICAM 1 , PECAM 1 , VCAM 1 , and E selectin 1 . ^^^ RESULTS : The adhesion molecules ICAM 1 , HLA DR , PECAM 1 , CD 3 , VCAM 1 , LFA 1 , and MAC 1 were selectively expressed in areas of corneal inflammation . ^^^ The ICAM 1 ligand was , in general , more diffusely distributed than its receptor LFA 1 , a beta 2 integrin found on leukocyte cell membranes . ^^^ The localization of the integrin MAC 1 , present on macrophages , neutrophils , and some lymphocytes , did not always parallel the staining pattern of ICAM 1 , suggesting promiscuity in its binding to other ligands besides ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Expression of LFA 1 and ICAM 1 on peripheral blood T cells and soluble CD 2 levels in serum during acute Kawasaki disease ] . ^^^ To clarify the activation of peripheral blood T cells in Kawasaki Disease ( KD ) patients , we investigated whether the expression of lymphocyte function associated antigen 1 ( LFA 1 ) and / or intercellular adhesion molecule 1 ( ICAM 1 ) on peripheral blood T cells and serum levels of soluble CD 2 ( sCD 2 ) increase during the acute stage . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Cells from BZSLL displayed a higher rate of expression and / or a stronger intensity of LFA 1 , LFA 3 , ICAM 1 , and BL CAM and a lower density of H CAM and LAM 1 homing receptors , as opposed to SLL or CLL . ^^^ A lower intensity of H CAM along with a stronger expression of LFA 1 , LFA 3 , ICAM 1 , and BL CAM were also detected by comparing BZSLL with ILL / MZL . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Within such plaques , microglial aggregates were stained intensely for leukocyte function associated antigen 1 ( LFA 1 ) , the adhesion molecule for ICAM 1 . ^^^ The LFA 1 / ICAM 1 system appears to play an important role in the interaction of astrocytes and microglia in several neurological diseases . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The molecules we analyzed included the VLA family VLA 1 6 , the leukocyte integrins LFA 1 , Mac 1 and p 150 , 95 , the immunoglobulins LFA 3 , CD 2 , ICAM 1 and VCAM 1 and the selectin ELAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In this study we report the expression of adhesion molecules ( CD11a , CD11c , CD 18 and ICAM 1 ) , lymphocyte surface markers ( CD 3 , CD 4 , CD 8 and CD 22 ) and HLA DR molecules in 25 epiretinal membranes obtained from eyes undergoing vitrectomy for the treatment of retinal detachment complicated by epiretinal membrane formation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Farage cells do not express surface immunoglobulins ( sIg ) but display a high level of CD 19 , CD 21 , CD 22 , CD 23 , CD 39 , CD 40 B cell antigens , and various adhesion proteins , such as CD11a ( LFA 1 ) , CD 29 ( VLA 4 ) , CD 44 , CD 54 ( ICAM 1 ) , and CD 58 ( LFA 3 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
MNC adherence to endothelial cells was not blocked by antibodies against the intercellular adhesion molecule 1 ( ICAM 1 ) , but was blocked by lymphocyte function associated antigen 1 ( LFA 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The stationary adhesions and migration are heavily dependent on two members of the CD 18 ( beta 2 ) integrin family , CD11a / CD18 and CD11b / CD18 , on the neutrophil surface , intercellular adhesion molecule 1 ( ICAM 1 ) on ECs , and endothelial derived chemoattractants such as interleukin 8 ( IL 8 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In fact , a receptor ligand interaction occurs between CD11a / CD18 ( LFA 1 ) and CD 2 molecules , expressed on the effector cells , and the respective counterparts on target cells , i . e . , ICAM 1 , ICAM 2 , or LFA 3 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Finally , both LFA 1 ( CD11a / CD18 ) and Mac 1 ( CD11b / CD18 ) beta 2 integrins were required as well as the endothelial adhesion molecules E selectin and ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The leukocyte specific integrins CD11a / CD18 ( lymphocyte function associated antigen 1 ( LFA 1 ) ) and CD11b / CD18 ( complement type 3 receptor ( Mac 1 ) ) , which bind to the intercellular adhesion molecules ICAM 1 and ICAM 2 , play a key role in adhesion . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
AML cells from the majority of patients with leukaemia at diagnosis or relapse expressed the ligand pair LFA 1 and ICAM 1 , the CD 2 ligand LFA 3 , alpha and beta chains of the integrins VLA 4 , VLA 5 and VLA 6 , and the hyaluronate receptor CD 44 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The LFA 1 integrin mediates its function in leukocyte intercellular interactions by recognition of at least one of its three identified counter receptors , intercellular adhesion molecules 1 ( ICAM 1 ) , 2 , and 3 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
However , a decrease in CD 23 , CD11a ( lymphocyte function associated antigen LFA 1 ) , and CD 58 ( LFA 3 ) expression and an increase in CD 38 ( a plasma cell associated antigen ) , CD 54 ( intracellular adhesion molecule ICAM 1 ) , and HLA class 1 in the tumor cells relative to the LCLs was observed . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The adhesion pathway triggered by the IFN inducible protein Leu 13 required metabolic energy and an intact cytoskeleton but was not dependent on : 1 ) new protein synthesis ; 2 ) protein kinase C , protein kinase A , or tyrosine kinase activities ; or 3 ) the function of known adhesion molecules including LFA 1 , ICAM 1 , CD 44 , or VLA 4 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Superantigen driven proliferation in the presence of IFN gamma treated keratinocytes was significantly inhibited ( 70 90 % reduction ) by mAb against the LFA 1 alpha or beta chain or ICAM 1 . ^^^ In addition to previous defined roles for class 2 MHC Ag , stimulation of LFA 1 on the T cells by ICAM 1 on the keratinocytes also plays an important costimulatory role in this superantigen mediated response . ^^^ These data demonstrate that 1 ) keratinocytes can serve as accessory cells for T cell proliferation using a variety of different stimuli , 2 ) the LFA 1 / ICAM 1 interaction plays a major role in keratinocyte mediated costimulation , and 3 ) previous reports in which IFN gamma treated keratinocytes failed to support T cell proliferation to nominal or alloantigens , may reflect impaired Ag presentation via class 2 MHC molecules , rather than lack of necessary costimulatory signals . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Cell surface molecules LFA 1 , ICAM 1 , HLA DR , CD 28 , and B 7 were also involved in the T B cell interaction since mAb to any of these molecules inhibited the gp 160 induced B cell differentiation response . gp 160 also induced IL 6R and CD 23 molecule expression on B cells when added to cultures of T plus B cells ; there was CD 23 expression only in cells that formed conjugates with T cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
These indices of myocardial stunning were reversed after blocking neutrophil endothelial cell interaction with monoclonal antibodies against CD 18 or ICAM 1 receptors , and significant improvements were also obtained after either thromboxane or leukotriene receptor blockade . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
This adherence was likely mediated by the beta 2 integrin , Mac 1 , and could be specifically inhibited with monoclonal antibodies to ICAM 1 and to the integrin beta 2 subunit . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The composition of inflammatory cells as well as the expression of cell adhesion molecules ( LFA 1 / CD11a , ICAM 1 / CD54 , CD 36 ) in jejunal biopsies from children with active , untreated coeliac disease ( CD ) has been analyzed and compared with control biopsies . ^^^ ICAM 1 was found on most cells in the lamina propria ; many of them expressed LFA 1 as well . ^^^ The results indicate that adhesion molecules other than LFA 1 , ICAM 1 and CD 36 may be involved in the cellular interactions occurring in the intraepithelial compartment in CD ; the lack of ICAM 1 and the reduced expression of LFA 1 on intraepithelial lymphocytes might reflect a defective activation of these cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Preincubation of the IL 3 treated cultures with anti IL 3 , anti leukocyte function associated antigen 1 ( LFA 1 ) alpha chain ( CD11a ) , and anti intercellular adhesion molecule 1 ( ICAM 1 ) inhibited cell fusion . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Both immunohistochemical analysis and the reverse transcripts polymerase chain reaction ( RT PCR ) were used to analyze the expression of VCAM 1 , ICAM 1 , ELAM 1 , very late antigen 4 ( VLA 4 [ alpha 4 , beta 1 ] ) , lymphocyte function associated antigen 1 ( LFA 1 ) , interferon gamma ( IFN gamma ) , tumor necrosis factor ( TNF ) , and interleukin 1 beta ( IL 1 beta ) . ^^^ Cytokine mediated up regulation of VCAM 1 and ICAM 1 that facilitates the recruitment of VLA 4 and LFA 1 expressing T cells might contribute to lymphoid cell infiltration in the salivary and lacrimal glands in SS . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Transendothelial migration in vitro is markedly inhibited by monoclonal antibodies against CD 18 integrins or ICAM 1 . ^^^ Monoclonal antibodies against the selectins , CD 18 , CD11a , CD11b , and ICAM 1 have all been shown to significantly reduce the influx of neutrophils into sites of inflammation in various animal models . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Furthermore , ICAM 1 , which is ligand for LFA 1 molecules , was also expressed on eosinophils with hypereosinophilia . ^^^ To extend our understanding of high intensity expression of adhesion molecules ( CR 3 , LFA 1 alpha , LFA 1 beta , ICAM 1 ) on hypodense eosinophils , we examined whether chemotactic agents or eosinophilopoietic growth lymphokines ( IL 3 , GM CSF , IL 5 ) is involved in adhesion molecule expression using an eosinophilic cell line named EoL 3 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
On peripheral blood lymphocytes slightly reduced expression of HLA DR , LFA 1 , CD 23 and ICAM 1 was seen after 100 days . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The immunopathogenesis of EAAU was studied by immunohistochemical detection of immune cells and the expression of Ia , ICAM 1 and LFA 1 antigens . ^^^ Immunohistology of the enucleated eyes was performed with monoclonal antibodies W3 / 25 ( CD 4 ) , OX 8 ( CD 8 ) , ED 2 ( macrophage ) , OX 33 ( B cell ) , OX 6 ( Ia ) , IA 29 ( ICAM 1 ) and WT . 1 ( LFA 1 ) . ^^^ An increase in ICAM 1 expression was first noted on the epithelial cells of the uveal tract and RPE on day 9 post immunization and preceded LFA 1 and Ia upregulation by approximately 2 days . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The role of the cell adhesion molecules , LFA 1 and ICAM 1 , in intraocular tumor rejection was examined using four different syngeneic intraocular regressor tumors and four different inbred mouse strains . ^^^ The effect of anti LFA 1 and anti ICAM 1 antibody treatment on the rejection of metastases arising from intraocular P 91 tumors was also examined and found to be highly dependent upon normal LFA 1 function since antibody treatment with anti LFA 1 prevented the rejection of metastases . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Control intestinal macrophages seemed to poorly express the adhesion molecules required for cell to cell adhesion and fusion , namely ICAM 1 and LFA 1 . ^^^ CD macrophages seemed to highly express both ICAM 1 and LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
METHODS : A panel of four human colon tumor cell lines ( HT 29 , SW 620 , HCT 15 , and LoVo ) , A 375 human melanoma cells , and human umbilical vein endothelial cells ( HUVEC ) were cultured in the presence of levamisole and examined by solid phase enzyme immunoassay to determine the level of expression of MHC class 1 , intercellular adhesion molecule 1 ( ICAM 1 ) , vascular cell adhesion molecule 1 ( VCAM 1 ) , leukocyte integrin VLA 4 , and lymphocyte functional antigen ( LFA 1 ) molecules . ^^^ The HCT 15 and LoVo colon tumor cells had very low basal MHC expression Levamisole ( 1 micrograms / mL ) increased VCAM 1 expression on HT 29 and SW 620 colon tumor cells to 4 . 3 and 2 . 4 times ( P < . 05 in each case ) control levels , respectively , doubled ICAM 1 expression on HT 29 cells ( P < . 05 ) , and increased LFA 1 expression on HT 29 , LoVo , and A 375 cells to 2 . 1 , 3 . 2 , and 1 . 8 ( P < . 05 in each case ) times control levels , respectively . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The ability of IFN gamma to induce the expression of adhesion molecules such LFA 1 and ICAM 1 on peripheral blood leukaemic cell surfaces may suggest its use in the induction therapy of CML patients . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Conversely , the first domain of ICAM 1 appears sufficient to bind activated LFA 1 . ^^^ The second domain of MAdCAM 1 can also support activation dependent LFA 1 binding to domain 1 of ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
ICAM 1 expressing pocket epithelium , LFA 1 expressing T cells in gingival tissue and gingival crevicular fluid as features characterizing inflammatory cell invasion and exudation in adult periodontitis . ^^^ In an attempt to clarify the relationship between the ICAM 1 ( CD 54 ) expression of pocket epithelium in gingiva and the infiltrating lymphocyte population , we carried out an analysis of CD11a+ ( LFA 1 alpha ) , CD25+ ( IL 2R alpha ) and CD4+ ( Th ) cells subjacent to ICAM 1 expressing pocket epithelia and CD11a+CD25+CD4+ cells in gingival crevicular fluid ( GCF ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In this study the expression of CAMs ( LFA 1 , LFA 3 , ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To determine the role of various adhesion molecules during the processes of lymphocyte binding and transmonolayer migration ( diapedesis ) , lymphocytes were treated with monoclonal antibody ( mAb ) specific for CD11a ( alpha L subunit of leucocyte functional antigen 1 ; LFA 1 ) , CD 18 ( beta 2 subunit of leucam family ) and CD49d ( alpha 4 subunit of very late activation antigen 4 ; VLA 4 ) and EC with mAb specific for CD 54 ( intercellular adhesion molecule 1 ; ICAM 1 ) and CD 106 ( vascular cell adhesion molecule 1 ; VCAM 1 ) . ^^^ Binding of the highly adhesive but non migratory Con A activated lymphocytes was inhibited by mAb to CD11a ( reduced to 73 % and 65 % of control lymphocyte adhesion ) and CD 18 ( 42 % and 54 % ) on non activated and IL 1 beta treated EC , respectively , but not by mAb to ICAM 1 or VCAM 1 . ^^^ Diapedesis of the highly migratory T cell line lymphocytes was also blocked by antibodies to CD11a ( reduced to 11 % and 10 % of control T cell migration ) , CD 18 ( 29 % and 43 % ) but in addition was also inhibited by anti ICAM 1 ( 17 % and 53 % ) on non activated and IL 1 beta treated EC , respectively . ^^^ Lymphocyte adhesion and transendothelial migration in the central nervous system : the role of LFA 1 , ICAM 1 , VLA 4 and VCAM 1 . off . ^^^ These studies indicate that lymphocyte adhesion to central nervous system ( CNS ) vascular EC is largely dependent on LFA 1 but not through its interaction with ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Phorbol ester ( TPA ) induced increase in cell surface expression of adhesion structures , i . e . intercellular adhesion molecule 1 ( ICAM 1 , CD 54 ) , beta 2 integrin LFA 1 ( CD11a ) , complement regulatory cell membrane protein protein ( CD 59 ) and leukocyte common antigen ( CD 45 ) in human erythroid / myeloid leukemia cell line K 562 was inhibited by staurosporine , an inhibitor with broad , non selective protein kinase inhibitory profile , but not by CGP 41 , 251 , a benzoylated staurosporine derivative with the selective protein kinase C ( PKC ) inhibitory activity . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Among the mAb against counter receptors for ICAM 1 tested , anti CD11a mAb suppressed IgE synthesis , while anti CD 18 mAb and anti CD 43 mAb had little effect . ^^^ These results indicate that B cell B cell interaction through ICAM 1 and one of its counter receptors , LFA 1 , regulates IgE synthesis by modulating C epsilon germ line transcription . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Anti PECAM Abs added to NK cells caused a 2 . 5 to 4 fold increase in the binding of these cells to monolayers of EC or 3T3 cells transfected with ICAM 1 , and this was inhibited by a mAb against CD 18 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Blocking of adhesion molecules ICAM 1 , LFA 1 alpha , and / or LFA 1 beta reduced adhesion up to 34 % . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
OBJECTIVE : The aim of this study was to see whether tolerance could be induced by simultaneous administration of monoclonal antibodies ( MoAbs ) to intercellular adhesion molecule 1 ( ICAM 1 ) and leukocyte function associated antigen 1 ( LFA 1 ) after transplantation of fetal small bowel between fully incompatible mice strains . ^^^ In the other allogeneic group ( n = 13 ) , both anti LFA 1 and anti ICAM 1 MoAbs ( 50 micrograms each / mouse / day ) were given intraperitoneally after transplantation for the first 4 weeks . ^^^ CONCLUSIONS : Specific tolerance for fetal bowel allografts could be induced by a relatively short term treatment with anti ICAM 1 and anti LFA 1 MoAbs . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Phenotypic analysis revealed that the T cells were positive for CD3+ , CD4+ , Leu 8 , CD45RO+ and T cell receptor ( TCR ) alpha beta+ , as well as showing strong surface expression of HLA DR , CD 25 , LFA 1 and ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
After whole blood lysis preparation , leukocytes were incubated with fluorescent antibodies to label LAM CD 11A / 18 ( LFA 1 ) , CD 11B / 18 ( Mac 1 ) , CD 11C / 18 ( p150 / 95 ) , and CD 54 ( ICAM 1 ) ( Becton Dickinson , San Jose , CA ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
This study showed that the alpha M beta 2 integrin , CR 3 ( Mac 1 , CD11b / CD18 ) served as the beta glucan receptor through one or more lectin sites located outside of the CD11b 1 domain that contains the binding sites for iC3b , ICAM 1 , and fibrinogen . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule ( ICAM 1 ) , a ligand for lymphocyte function associated antigen 1 ( LFA 1 ) , plays an important role in a variety of immune mediated mechanisms such as lymphocyte attachment to cultured Graves ' thyroid cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Allergen provocation reduced the proportion of T cells expressing the beta 2 integrin lymphocyte functional antigen 1 ( LFA 1 ) from 72 . 5 + / 30 to 43 . 9 + / 9 . 1 mean fluorescent units ( p < 0 . 01 ) and a similar trend in intercellular adhesion molecule 1 ( ICAM 1 ) ( p = 0 . 08 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Quantitative polymerase chain reaction Mac 1 analysis shows that LPS induced transcription of E selectin mRNA was dramatically suppressed by both 25 and 50 mg / kg of tepoxalin , whereas the level of ICAM 1 was only affected by 50 mg / kg of tepoxalin . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Leukocyte CD 18 receptors may be a better target than ICAM 1 ligands for reducing histologic evidence of cellular and vascular rejection in the rabbit . ^^^ Treatment with monoclonal antibody ( mAb ) to CD 18 was compared to antibody to ICAM 1 in a rabbit heterotopic heart transplant model to determine whether blocking the leukocyte receptor for ICAM 1 , CD 18 , was more effective than antibody targeting of the ligand for ICAM 1 . ^^^ Following transplantation , 28 recipient rabbits were randomized to receive either placebo , mAb to CD 18 , or mAb to ICAM 1 for 7 days until sacrifice . ^^^ The cellular rejection grade and percentage of arteries with vascular rejection were significantly lower in animals treated with anti CD 18 than with anti ICAM 1 . ^^^ As assessed by histology , antibody treatment was more effective in reducing both cellular and vascular rejection when directed at the leukocyte receptor CD 18 than the ICAM 1 ligand . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The effect of treatment with monoclonal antibody against ICAM 1 and CD 18 was studied . ^^^ Finally , the expression of ICAM 1 and CD 18 in the airway was investigated . ^^^ Our results show that eosinophil infiltration is important in LAR development and the treatment with antagonists of ICAM 1 and CD 18 may provide a therapeutic approach to reducing asthmatic symptoms . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Immunohistological examination revealed that the expression of ICAM 1 in sinusoidal endothelial cells and LFA 1 in hepatic macrophages was greater in both models than in normal rats preceding the development of hepatic necrosis . ^^^ We conclude that adhesion of activated macrophages to endothelial cells via LFA 1 and ICAM 1 in the hepatic sinusoids is essential for endothelial cell destruction in the C . parvum model , but can act protectively against provocation of fibrin deposition in the hepatectomy model . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Since binding of TK 1 to TNF alpha treated eEnd . 2 is mediated by the interaction of ICAM 1 and VCAM 1 ( on EC ) with their receptors LFA 1 and VLA 4 ( on T cells ) , we further investigated whether PGE 1 would modulate the expression of these molecules . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Blocking experiments using monoclonal antibodies directed against various adhesion molecules demonstrated that interactions between matched activated T lymphocytes and OECs were mediated by integrin dependent ( ICAM 1 / leukocyte function associated antigen 1 ( LFA 1 ) : VCAM 1 / very late antigen 4 ( VLA 4 ) ) and integrin independent ( CD 44 ) pathways , and revealed marked differences when comparing GO OECs and normal OECs . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
T cell adhesion to intercellular adhesion molecule 1 ( ICAM 1 ) is controlled by cell spreading and the activation of integrin LFA 1 . ^^^ We how that treatment with high concentrations of the divalent cation Mg2+ induces a high affinity state of LFA 1 , which is reflected in the binding of soluble ICAM 1 and correlates with the expression of the epitope recognized by mAb 24 . ^^^ These low affinity LFA 1 receptors are responsible for the initial contact with immobilized ligand because , unlike the Mg2+ stimulated receptors , adhesion is not blocked by soluble ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
They also compare the density of integrins ( CD 29 , CD49e , CD 41 , CD 51 and CD 61 ) and adhesive proteins from the group of `` homing ' ' receptors ( CD 44 ) and IgG `` superfamily ' ' ( LFA 1 , LFA 3 , ICAM 1 , N CAM ) and their changes after a single oral dose of a mixture of proteolytic enzymes ( Wobe Mugos , Wobenzym , MUCOS Pharma , FRG ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The expression of different types of adhesion molecule ( CD 44 , alpha 4 integrins , LFA 1 , ICAM 1 , CD 2 and L selectin ) on lymphocytes , NK cells , monocytes and granulocytes of rat blood was determined by flow cytometry . ^^^ Lymphocytes , NK cells , monocytes and granulocytes each expressed CD 44 , alpha 4 integrins , LFA 1 , ICAM 1 , CD 2 and L selectin in a pattern characteristic to cell type , but this did not correlate with the different ability of the leucocyte subsets to interact with the two types of HEV . ^^^ In conclusion , determining the expression of CD 44 , alpha 4 integrins , LFA 1 , ICAM 1 , CD 2 and L selectin on blood leucocytes alone is not sufficient to predict leucocyte / endothelium interaction in vivo . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
It does not cross react with antibodies directed against ICAM 1 , LFA 1 alpha , B 7 , HSA and VCAM 1 , suggesting the novelty of the protein . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We utilized a model of myelin basic protein ( MBP ) activation in vivo and MBP stimulated cultures in vitro to study the influence of TGF beta 1 on glial cell proliferation and ICAM 1 / leucocyte function associated antigen 1 ( LFA 1 ) expression , and to observe the antagonistic effects of TGF beta 1 and IFN gamma . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Immunohistochemical hepatic expression of intercellular adhesion molecule 1 ( ICAM 1 ) and lymphocyte function associated antigen 1 alpha ( LFA 1 alpha ) and the effect of monoclonal antibodies against both adhesion molecules on liver necrosis provoked after endotoxin administration was studied in these rats . ^^^ There were increased stains of ICAM 1 in endothelial cells and LFA 1 alpha in macrophages in the hepatic sinusoids in Propionibacterium acnes pretreated rats compared with normal rats . ^^^ Thus , adhesion between endothelial cells and activated macrophages in the hepatic sinusoids via ICAM 1 and LFA 1 alpha is essential for the initiation of massive hepatic necrosis of this type . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
ICAM 1 and LFA 1 were strongly expressed in lympho monocytic vasculitis . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
ICAM 1 and LFA 1 were strongly expressed . ^^^ ICAM 1 and LFA 1 were strongly expressed in the late phase , while gamma / delta T cells and the expression of 72 kD heat shock protein were significantly present only in leukocytoclastic CNV with documented infective etiology . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Integrin involvement in parotid interactions was indicated by inhibitory effects of anti HEBF ( PP ) , LFA 1 , ICAM 1 , and alpha 4 integrin antibodies as well as by the PMA enhanced adherence . ^^^ FACS analysis showed differences in parotid and submandibular endogenous lymphocyte adhesion molecule expression with greater percentages of L selectin , HEBF ( PP ) , alpha 4 integrin , LFA 1 , ICAM 1 , CD 44 , and Thy 1 positive cells present in parotid gland . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In contrast , antibodies to LFA 1 and ICAM 1 blocked CIK cell mediated tumor cell lysis . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
AH66F cells expressed leukocyte function associated antigen 1 ( LFA 1 ) and intercellular adhesion molecule 1 ( ICAM 1 ) , a ligand of LFA 1 , on the cell surface , while AH 130 cells had ICAM 1 alone . ^^^ The adhesion of M cells of AH66F cells was inhibited to the adhesion level of AH 130 cells by anti rat LFA 1 alpha and / or beta chain monoclonal antibody ( mAb ) and also by anti rat ICAM 1 mAb . ^^^ These results indicate that a part of the adhesion of AH66F cells to M cells is due to LFA 1 / ICAM 1 interaction , and we suggest that this characteristic feature of AH66F cells may be related to the malignant properties . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In contrast , the expression of VLA 6 , ( alpha ( 5 ) beta 3 ) , E cadherin , ICAM 1 and LFA 1 was reduced in this high metastatic line . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Expression of the adhesion molecules ICAM 1 , VCAM 1 , and E selectin and their ligands VLA 4 and LFA 1 in chronic venous leg ulcers . ^^^ In other systems , such binding is mediated by the interaction of adhesion molecules such as intracellular adhesion molecule 1 ( ICAM 1 ) , vascular cell adhesion molecule ( VCAM 1 ) and E selectin ( on ECs ) , and leukocyte function associated antigen 1 ( LFA 1 ) and very late activated antigen 4 ( VLA 4 ) ( on Leukocytes ) . ^^^ METHODS : Twenty seven biopsy specimens of inflamed dermatoliposclerotic skin adjacent to venous leg ulcers were stained immunohistochemically with monoclonal antibodies against ICAM 1 , VCAM 1 , LFA 1 , VLA 4 , and E selectin . ^^^ RESULTS : Specimens of leg ulcers caused by chronic venous insufficiency showed increased expression of ICAM 1 and VCAM 1 but not of E selectin on The expression of LFA 1 and VLA 4 on perivascular leukocytes was increased dramatically in comparison to healthy skin . ^^^ CONCLUSION : Upregulation of ICAM 1 and VCAM 1 on ECs may contribute to the increased adherence and extravasation of LFA 1 and VLA 4 positive leukocytes in chronic venous insufficiency . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The initial phase of this increased rate of egression was inhibited by antibodies to inter cellular adhesion molecule 1 ( ICAM 1 ) or CD 18 by 100 and 71 % , respectively . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Most TDC were LFA 1 ( CD11a ) + , CD18+ , ICAM 1 ( CD 54 ) + and CD 53 ( OX 44 ) + , but only certain subsets expressed CD11b and thymocyte markers Thy 1 , CD 2 , CD 4 and CD 8 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
METHODS : Cultured murine ISMCs were exposed to IFN gamma for 72 hours and analyzed for Mac 1 ( CD11B CD 18 ) antigen , MHC 2 , and ICAM 1 expression using enzyme linked immunosorbent assay and fluorescence activated cell sorter scan . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Neutrophil induced liver injury during endotoxemia is dependent on the adhesion molecules Mac 1 ( CD11b / CD18 ) on neutrophils and its counterreceptor on endothelial cells and hepatocytes , intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
ICAM 1 ( or CD 54 ) expression on epithelial cells is relevant for several reasons : ( a ) healthy individuals and patients with pollen allergy out of the pollen season do not express this molecule ; ( b ) ICAM 1 is the natural ligand of LFA 1 ( an integrin expressed on granulocytes ) , and ( c ) ICAM 1 is also receptor for rhinoviruses . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Lymphocytes activate adhesion to intracellular adhesion mlecule 1 ( ICAM 1 ) via leukocyte function associated antigen 1 ( LFA 1 ) , their major beta 2 integrin , in response to PMA ( phorbol 12 myristate 13 acetate ) without an increase in the number of receptors expressed . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The majority of the infiltrating lymphocytes expressed VLA 4 , LFA 1 , CD 44 and ICAM 1 , a variable fraction expressed Leu 8 and VLA 5 , and few or no cells were positive for VLA 1 , VLA 2 and VLA 6 . ^^^ However , at the single cell level , the expression of VLA antigens , LFA 1 , CD 44 and ICAM 1 was similar in both groups . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In the present study , we used a series of human / murine CD11a 1 domain chimeras , to localize a fourth 1 domain epitope ( IdeD ) , recognized by three independently derived anti CD11a antibodies that selectively block the binding of LFA 1 to ICAM 3 , but not to ICAM 1 . ^^^ Taken together , these data indicate that distinct regions of the CD11a 1 domain contain epitopes for antibodies that either selectively inhibit binding of LFA 1 to ICAM 3 , or interfere with both ICAM 1 and ICAM 3 binding of LFA 1 . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
It has been proposed that the extent of N linked glycosylation at Asn 240 and Asn 269 in the third domain of ICAM 1 may regulate the binding avidity of ICAM 1 to Mac 1 [ Diamond , M . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The B lymphoblastoid cell line JY undergoes homotypic aggregation in a lymphocyte function associated antigen 1 ( LFA 1 ) mediated , intracellular adhesion molecule 1 ( ICAM 1 ) dependent manner when stimulated with phorbol 12 myristate 13 acetate or anti LFA 1 antibodies . ^^^ Phosphorylation of p130cas was dependent on binding of LFA 1 to its ligand , ICAM 1 , as demonstrated by the use of anti ICAM 1 antibodies . ^^^ Several observations suggest that this event may be an important step in the signaling pathway initiated by LFA 1 . p130cas phosphorylation was rapidly reversible upon disengagement of the LFA 1 ICAM 1 complex and required cell adhesion since binding of phorbol 12 myristate 13 acetate stimulated JY cells to purified ICAM 1 or cross linking of either LFA 1 or ICAM 1 was not sufficient to induce phosphorylation of p130cas . ^^^ This observation suggests that adhesion mediated by the interaction of LFA 1 and ICAM 1 initiates a signaling cascade that involves the activation of protein tyrosine kinases and leads to the regulation of protein protein interaction via SH 2 domains , a key process shared with growth factor signaling pathways . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Enhanced T lymphocyte expression of LFA 1 , ICAM 1 , and the TNF receptor family member OX 40 in HgCl 2 induced systemic autoimmunity . ^^^ The levels of LFA 1 expression as well as the fractions of ICAM 1 + cells were significantly increased in all CD45RC defined subsets of CD4+ and CD8+ cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
METHODS : Immunohistochemical analysis was used to determine the distribution of the following adhesion molecules : lymphocyte function associated antigen 1 ( LFA 1 ; CD11a / CD18 ) , Mac 1 and p150 / 95 ( CD11bc / CD18 ) , intercellular adhesion molecule 1 ( ICAM 1 ) , and CD 44 in tissue sections from the ankle joints of rats with AIA . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Preincubation of ATRA treated NB 4 cells with MoAbs anti VLA 4 and anti LFA 1 , the VCAM 1 and ICAM 1 counter receptors respectively , resulted in a significant inhibition of adhesion . ^^^ Cytofluorimetric analysis of the NB 4 cell membrane molecules confirmed the increase under ATRA of VLA 4 , LFA 1 , MAC 1 and ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We investigated the expression of adhesion molecules ( CD 44 , VLA 5 , VLA 4 , LFA 1 , ICAM 1 , L selectin and c kit ) on bone marrow CD34++ cells from 16 CML patients by three colour flow cytometry . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The interaction of leukocyte function associated antigen 1 ( LFA 1 ) and intercellular adhesion molecules 1 ( ICAM 1 ) mediates cell adhesion and aggregation , which facilitate leukocyte trafficking to inflamed tissues and augment effector functions . ^^^ We investigated how LFA 1 / ICAM 1 mediated adhesion and aggregation are regulated in HL 60 cells induced to differentiate into neutrophils by retinoic acid ( RA ) . ^^^ Uninduced HL 60 cells did not bind to ICAM 1 even with stimulation by 12 0 tetradecanoyl phorbol 13 acetate , although they express LFA 1 on the cell surface . ^^^ The HL 60 cells transfected with the active form of Ras ( Val 12 ) exhibited LFA 1 / ICAM 1 dependent aggregation by RA stimulation without change in the avidity of LFA 1 . ^^^ These results suggest that distinct regulatory mechanisms control LFA 1 / ICAM 1 dependent adhesion and aggregation in HL 60 cells differentiating into neutrophils . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The adhesion molecule intercellular adhesion molecule 1 ( ICAM 1 ) , in addition to its membrane associated form ( mICAM 1 ) , also exists as a soluble form ( sICAM 1 ) . sICAM 1 is capable of binding to lymphocyte function associated antigen 1 ( LFA 1 ) molecules , and production of sICAM 1 is therefore thought to have immunomodulatory consequences . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Formalin fixed and paraffin embedded samples were serially sectioned and stained with antibodies against nm 23 , integrin beta 1 subfamily members ( alpha2beta1 , alpha3beta1 and alpha4beta1 ) , LFA 1 , ICAM 1 , sialyl Lewis ( 10 ) ( sLex ) and CD44H , V 3 , and V 6 . ^^^ The percent of tumors expressing each adhesion molecule was as follows : alpha2beta1 , 27 . 3 % ; alpha3beta1 , 20 . 0 % ; alpha4beta1 , 14 . 5 % ; LFA 1 , 14 . 5 % ; ICAM 1 , 12 . 7 % ; sLex , 67 . 3 % ; CD44H , 55 . 5 % ; CD44V3 , 20 . 0 % ; and CD44V6 , 4 . 5 % . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
An aspartic acid located at position 137 , which is essential to ICAM 1 / LFA 1 interactions ( Edwards , C . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In this study , we examined expressions of several adhesion molecules ( AdMs ) , i . e . leukocyte function antigen 1 ( LFA 1 : CD11a / CD18 ) , Hermes homing receptor ( CD 44 ) and intercellular adhesion molecule 1 ( ICAM 1 : CD 54 ) , on leukemia cells from 51 adult patients with newly diagnosed acute myeloid leukemias ( AMLs ) to elucidate clinical significance of these AdM expressions . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Ctx , but not Ptx , inhibited the LFA 1 dependent adhesion of NK cells to tumor target cells which constitutively express the intercellular cell adhesion molecule 1 ( ICAM 1 ) and to NIH / 3T3 mouse fibroblasts stably transfected with human ICAM 1 . ^^^ These findings , together with the absence of any detectable effect of Ctx on the constitutive phosphorylation of LFA 1 alpha , suggests that this toxin modifies the avidity of LFA 1 for ICAM 1 by acting on LFA 1 cytoskeletal protein association . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Our purpose was to assess the expression of cell membrane antigens ( CD 3 , CD 4 , CD1a , CD 36 ) , of adhesion receptors ( leukocyte function adhesion 1 , LFA 1 , endothelial leukocyte adhesion molecule 1 , ELAM 1 ) intercellular adhesion molecule 1 , ICAM 1 ) , and the intercellular relationships in the early phase of the disease . ^^^ In the very early phase of the disease the adhesion receptors LFA 1 and ICAM 1 were expressed intensely by all infiltrating cells ; the adhesion receptors ICAM 1 and ELAM 1 were expressed by endothelial cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The ability of soluble ICAM 1 ( sICAM 1 ) to inhibit ICAM 1 / LFA 1 adhesion events has been reported previously by numerous investigators . sICAM 1 has been demonstrated to inhibit various in vitro assays at concentrations ranging from 2 nM to greater than 40 microM . ^^^ Considering the potential clinical importance of the interaction between ICAM 1 and its receptor , LFA 1 , it is necessary to understand this receptor ligand interaction at a molecular level . ^^^ These results indicate that while the monomeric sICAM 1 / LFA 1 interaction may involve only a moderate binding affinity , multimeric ICAM 1 present on a cell surface may bind cell surface immobilized LFA 1 with very high avidity . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Despite this difference , NEUT and EOS adhesion was reduced by blocking Abs to epithelial ICAM 1 or granulocyte CD 18 adhesion proteins ( p < 0 . 01 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Our results have demonstrated that first , the PMN endothelial cell interaction mediated by leukocyte function associated antigen 1 ( LFA 1 ) and intercellular adhesion molecule 1 ( ICAM 1 ) was crucial for the generation of 6 19 RF mAb induced skin leukocytoclastic vasculitis , but not for glomerular lesions ; second , PMN played an active role in the development of `` wire loop ' ' glomerular lesions ; in the absence of the PMN glomerular infiltration , 6 19 RF mAb induced a different type of glomerular lesions , characterized by voluminous intracapillary thrombi and mesangial deposits , but not subendothelial deposits ; and third , the activation of the complement system did not appear to play a major role in both skin and glomerular lesions . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The treatment with monoclonal antibodies against intercellular adhesion molecule 1 ( ICAM 1 ) and lymphocyte function associated antigen 1 ( LFA 1 ) prevented the development of insulitis with abnormal glucose tolerance in a dose dependent manner . ^^^ These results suggest that ICAM 1 and LFA 1 molecules may , at least in part , participate in islet cell damage , resulting in reovirus type 2 induced diabetes like syndrome . ^^^ The role of ICAM 1 and LFA 1 molecules on the development of insulitis is discussed . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
T lymphocytes can be drawn to the area of macrophage activity by the expression of ICAM 1 and LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Blocking of ICAM 1 and LFA 1 in rat liver transplantation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Difference in immunosuppressive effects of antibodies to ICAM 1 and LFA 1 between hepatic and cardiac allografts . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Dose dependent immunosuppressive effects of antibodies to ICAM 1 and LFA 1 on hepatic allografts . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
A large proportion of murine fetal thymus ( FT ) cells obtained at Day 13 of gestation , which are c kit+ , express the adhesion molecules Pgp 1 , VLA 4 , LFA 1 , and ICAM 1 on their surface at high levels . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Using immunofluorescence analysis we detected CD 8 , LFA 1 , and ICAM 1 on the surface of 10BK . 1 cells , but no CD 3 or T cell receptor ( TCR ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Here we show that LFA 1 and Mac 1 can bind to ICAM 1 in the mouse as well . ^^^ Interestingly , we observed that binding of murine LFA 1 dominates over Mac 1 for binding to ICAM 1 . ^^^ Using three different murine macrophage cell lines that express distinct levels of LFA 1 and Mac 1 on their cell surface , we could only detect Mac 1 dependent adhesion to ICAM 1 when little or no LFA 1 is expressed on the cell surface . ^^^ When LFA 1 and Mac 1 are expressed at similar levels , the LFA 1 / ICAM 1 interaction dominates over Mac 1 / ICAM 1 interaction , indicating that there is a competition of LFA 1 and Mac 1 for ICAM 1 binding . . ^^^ Both human integrin receptors Mac 1 ( CD11b / CD18 , CR 3 ) and lymphocyte function associated antigen ( LFA ) 1 ( CD11a / CD18 ) have been demonstrated to bind human intercellular adhesion molecule 1 ( ICAM 1 ) ( CD 54 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To determine whether direct cell to cell interaction involving adhesion molecules was involved in increased NO induction , the ability of neutralizing monoclonal antibodies directed against intercellular adhesion molecule type 1 ( ICAM 1 ) and leukocyte function antigen 1 ( LFA 1 ) to suppress NO production and lower intracellular chlamydial inhibition was investigated . ^^^ It was found that monoclonal antibodies against ICAM 1 / LFA 1 could significantly reduce the capacity of a protective CD4+ type 1 ( Thl ) clone ( clone 2 . 14 0 ) to inhibit the intracellular growth of the C . trachomatis agent of mouse pneumonitis ( MoPn ) . ^^^ Also , paraformaldehyde fixed clone 2 . 14 0 could enhance NO induction and chlamydial inhibition mediated by IFN gamma , and this effect could be reversed by anti ICAM 1 / LFA 1 antibodies . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The mNI 11 induced aggregation was blocked by the addition of ethylenediaminetetraacetate , and also when incubated at 4 degrees C . mAbs to CD11a / CD18 ( lymphocyte function associated antigen 1 ; LFA 1 ) and CD 54 ( intercellular adhesion molecule 1 ; ICAM 1 ) completely blocked the LPS U 937 cell aggregation induced by mNI 11 . ^^^ The mNI 11 induced LPS U 937 cell adhesion to HUVECs was not reduced in the presence of LFA 1 ( CD11a / CD18 ) or ICAM 1 ( CD 54 ) mAbs . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We show that , after a primary proliferative response to SEB , LFA 1 / ICAM 2 adhesive interactions can play a part in AICD following SEB rechallenge , while B 7 and ICAM 1 mediated interactions are not essential for this process . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In addition , groups of mice received intravenous ( i . v . ) infusion following transplantation with a mixture of monoclonal antibodies ( mAb ) to vascular adhesion molecule 1L : very late activation antigen 4 ( VCAM 1 : VLA 4 ) or intracellular adhesion molecule 1 : lymphocyte function associated antigen 3 ( ICAM 1 : LFA 1 ) . ^^^ The mAb treatment protocol which produced optimum increases in graft survival in naive versus immune mice was different , with anti LFA 1 : ICAM 1 superior for naive mice compared with anti VLA 4 : VCAM 1 , and vice versa for immune animals . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
This study was designed to investigate the role of LFA 1 / ICAM 1 interactions in homotypic adhesion and proliferation of CD 40 activated human B lymphocytes . ^^^ The addition to the culture medium of LFA 1 and ICAM 1 antibodies inhibited homotypic B lymphocyte adhesion . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Thus , the interaction between lymphocyte function associated antigen 1 ( LFA 1 ) and intercellular adhesion molecule 1 ( ICAM 1 ) was crucial for the F 2 dependent T cell mediated cytotoxicity against oral epithelial cells , while HLA DR and CD 2 molecules are not necessarily involved in the cytotoxicity observed . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
At this time there was a substantial increase in the number of T lymphocytes and eosinophils expressing ICAM 1 and the integrins VLA 4 and LFA 1 , implicating these adhesion molecules in inflammatory cell recruitment to the airways . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The distribution of RCMV , intercellular adhesion molecule 1 ( ICAM 1 ) and its leucocyte receptor CD11a ( LFA 1 ) were investigated by immunohistochemistry . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
ICAM 1 , VCAM 1 , and MAdCAM 1 expressed in choroid plexus epithelial cells mediated binding of lymphocytes via their known ligands LFA 1 and alpha 4 integrin , respectively . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Their splenic microarchitecture is disorganized , characterized by the loss of the clearly defined marginal zone , ill defined T and B cell areas , and absence of MAdCAM 1 and reduced ICAM 1 , VCAM 1 and Mac 1 expression . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The ratios of LFA 1 alpha , beta , ICAM 1 , HLA DP among SLE patients were significantly higher when compared with normal healthy controls , and the significant correlation between the ratio of LFA 1+ T cells and HLA DP+ T cells , and LFA 1+ T cells and ICAM 1+ B cells was recognized . ^^^ These results may suggest that LFA 1 , ICAM 1 is related to the mutual action between activated T cells and B cells . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Immunohistochemical studies of tumor infiltrating lymphocytes in renal cell carcinoma showed a significantly high incidence of CD 3 , CD 8 , CD11b and ICAM 1 in the administration group , while CD 4 , LFA 1 and Ber MAC 3 were increased without significance ( p < 0 . 05 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
METHODS : The production and distribution of proteins that may be implicated in inflammation was examined by immunohistochemistry of sections of left ventricles from 1 month and 4 month renovascular hypertensive and age matched control rats using antibodies against ICAM 1 , LFA 1 , TGF beta 1 , PDGF A , T and H kininogens , IgG , IgM , C 3 , and C5b 9 . ^^^ There were more ICAM 1 and LFA 1 in the ventricles of hypertensive than in control rats at 1 month , but the ICAM 1 expressions in hypertensive and control rats were similar at 4 months . ^^^ CONCLUSION : Thus , inflammatory cells infiltrate the cardiac tissue of renovascular hypertensive rats as in the case of spontaneously hypertensive rats and these cells may use the ICAM 1 / LFA 1 system to infiltrate , but neither TGF beta 1 and PDGF A , nor the kininogen system seem to be associated with cardiac fibrogenesis . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Antibody blocking studies demonstrated that the vascular adhesion molecules ICAM 1 , VCAM 1 and E selectin and the leucocyte integrin LFA 1 all contributed to lymphocyte binding to endothelium activated by IL 6 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Peripheral blood lymphocyte phenotype ( CD 3 , CD 4 , CD 8 , CD 20 , CD45RA and CD45RO ) and adhesion molecule phenotype ( CD11a / LFA 1 alpha , CD18 / LFA 1 beta and CD54 / ICAM 1 ) were investigated in 35 patients with systemic lupus erythematosus ( SLE ) by using flow cytometric analysis and dual immunofluorescence staining method . ^^^ These data suggest that adhesion molecule LFA 1 and ICAM 1 may play an important role in the pathogenesis of SLE . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule 1 ( ICAM 1 ) is a member of the immunoglobulin superfamily that interacts with two integrins , LFA 1 and Mac 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Glomerular expression of intercellular adhesion molecule 1 ( ICAM 1 ) ( CD 54 ) and membrane cofactor protein ( MCP ; CD 46 ) and positive infiltrating cells in leukocyte function associated antigen 1 ( LFA 1 ) alpha ( CD11a ) and C3bi receptors ( CR3 / CD11b , CR4 / CD11c ) were examined by the indirect immunoperoxidase method on 43 sets of repeated renal biopsy specimens from patients with immunoglobulin A nephropathy . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Patients ' sera induced an increase in surface expression of CD11a and CD 18 on normal neutrophils and intercellular adhesion molecule 1 ( ICAM 1 ) expression on HUVECs . ^^^ Monoclonal antibodies to CD11a , CD11b , CD 18 , and ICAM 1 caused varying degrees of inhibition of neutrophil adhesion . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Fibrinogen is a ligand for Intercellular Adhesion Molecule 1 ( ICAM 1 ) , and enhances monocyte endothelial cell interaction by coupling Mac 1 on monocytes to ICAM 1 on endothelial cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Furthermore , LFA 1 and ICAM 1 , but not ICAM 3 ( the third ligand for LFA 1 ) were observed in the interstitial foam cells . ^^^ LFA 1 and ICAM 3 were observed mainly in interstitial infiltrating cells , but ICAM 1 was observed to a much lesser extent in these cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
LFA 1 ( CD11a / CD18 ) and ICAM 1 ( CD 54 ) antibodies attenuate superoxide anion release from polymorphonuclear leukocytes in rats with experimental acute pancreatitis . ^^^ The inhibitive effects of anti CD11a / CD18 ( LFA 1 ) and anti CD 54 ( ICAM 1 ) antibodies on the generation of superoxide anion ( O 2 ) by polymorphonuclear leukocytes ( PMNs ) was elucidated in rats induced with experimental acute pancreatitis . ^^^ In normal rats , although LFA 1 antibody attenuated the generation of O 2 , ICAM 1 antibody did not . ^^^ However , in pancreatitis rats , both LFA 1 and ICAM 1 antibodies reduced the generation of O 2 by PMNs isolated from blood and the peritoneal cavity . ^^^ These results showed not only that both LFA 1 and ICAM 1 antibodies have a protective effect on the generation of O 2 , but also that LFA 1 has a direct inhibitive effect on the generation of O 2 by PMNs in this model . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The injection of a MoAb ( 1 mg / kg ) , 15 min prior to the LPS challenge , resulted in ( 1 ) total inhibition of LA , when MoAb to rat L section , LFA 1 beta and VLA 4 were used , ( 2 ) a moderate effect with LFA 1beta and Mac 1 MoAb , and ( 3 ) only a weak influence on LA by the MoAb to rat ICAM 1 ( 1 mg / kg ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The decrease in adhesion was not mediated by a change in adhesion molecule expression as LFA 1 and VLA 4 expression on T cells and ICAM 1 and VCAM 1 expression on endothelial cells were not changed by catecholamine stimulation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Combined use of antibodies against lymphocyte function associated antigen 1 ( LFA 1 ) and intercellular adhesion molecule 1 ( ICAM 1 ) strongly inhibited the migration of T lymphocytes to the peripheral LNs . ^^^ These results indicate that L selectin plays an essential role in migration of both T and B lymphocytes into peripheral LNs but LFA 1 / ICAM 1 and VLA 4 / VCAM 1 play different roles in compartmentalization of T and B lymphocytes in the peripheral LNs . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Binding of neutrophils could be blocked with antibodies against L selectin , LFA 1 , CR 3 and anti ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In the present study , we examined the role of ICAM 1 / LFA 1 adhesion molecules in corneal allograft rejection and evaluated the immunological specificity of monoclonal antibodies ( mAbs ) in preventing allograft rejection in mice . ^^^ METHODS : The allografted mice were intraperitoneally injected with 100 micrograms / day of the following mAbs : a control mAb , anti ICAM 1 mAb , anti LFA 1 mAb , or a mixture of anti ICAM 1 and anti LFA 1 mAbs from 1 day before to 7 days after surgery . ^^^ The expression of ICAM 1 and LFA 1 molecules in the grafted cornea was studied immunohistochemically . ^^^ The corneas from a syngeneic donor or a third party strain were transplanted 4 weeks after the initial keratoplasty onto the mice treated with both anti ICAM 1 and anti LFA 1 mAbs . ^^^ RESULTS : The allografts treated with anti LFA 1 mAb alone or both anti ICAM 1 and anti LFA 1 mAbs remained transparent for more than 2 weeks , and the survival rate at 8 weeks was 40 % in both groups . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In contrast , 3A9 cells peeled from the ICAM 1 bilayer , and held firmly on LFA 1 bilayers ; in a manner dependent on filamentous actin . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The aim of this study was to investigate the effects of intra arterial infusion of low doses of monoclonal antibodies ( Mabs ) against adhesion molecules ( the neutrophil CD 18 integrins , and the endothelial adhesion molecule , ICAM 1 ) on reperfusion injury in skeletal muscle . ^^^ The results of the viability assessment ( control 20 . 9 ( 0 47 . 5 ) % [ median ( range ) ] , anti CD 18 30 . 5 ( 3 . 0 89 . 4 ) % , anti ICAM 1 27 . 9 ( 7 . 8 78 . 1 ) % and anti CD 18 combined with anti ICAM 1 45 . 2 ( 15 . 6 92 . 3 ) % ) showed no significant differences between groups , while analysis of MPO in the postischaemic muscle showed that the anti ICAM 1 Mab reduced neutrophil infiltration significantly . ^^^ These results suggest that low doses of anti CD 18 and anti ICAM 1 Mabs do not reduce reperfusion injury in skeletal muscle but may help to protect against systemic effects of severe trauma . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
These results show that slow cluster formation shares the LFA 1 and phorbol ester requirements of the rapid adhesion of T cells requiring LFA 1 and ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Immunosuppressive effect of monoclonal antibodies to ICAM 1 and LFA 1 on peripheral nerve allograft in mice . ^^^ After nerve allografting , 18 recipients were treated with both anti intercellular adhesion molecule 1 ( ICAM 1 ) and anti lymphocyte function associated molecule 1 ( LFA 1 ) monoclonal antibodies in low or high dose . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Our results also indicate that viral pathogenicity is associated with increased migration of inflammatory cells expressing VLA alpha 4 , LFA 1 alpha , Mac 1 alpha , ICAM 1 , and beta 2 integrin into the intestinal mucosa . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule 1 ( ICAM 1 ) is a ligand for lymphocyte function associated antigen 1 alpha ( LFA 1 alpha ) , and it has been shown to be required for leukocyte migration into inflamed areas . ^^^ The purpose of this report was to investigate the role of the ICAM 1 / LFA 1 alpha pathway in a rat model of acid aspiration induced injury . ^^^ Animals received 3 . 0 ml / kg HCI ( 0 . 1N ; pH , 1 . 0 ) intratracheally pretreated with control monoclonal antibodies ( mAbs ) ( HCI group ) or anti ICAM 1 and LFA 1 alpha mAbs ( Test group ) . ^^^ The administration of mAbs to ICAM 1 and LFA 1 alpha after HCI instillation partially attenuated HCI induced responses . ^^^ These observations suggest that the ICAM 1 / LFA 1 alpha pathway might be involved in the pathogenesis of adult respiratory distress syndrome caused by acid aspiration . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
This decrease was blocked by anti ICAM 1 anti CD 18 and the inhibition of nitric oxide synthesis . ^^^ TNF alpha but not IFN gamma was detected in the supernatant of co culture between KC and AH 70 cells , and this production was partially inhibited by anti ICAM 1 and anti CD 18 . ^^^ The activity of inducible nitric oxide synthase in Kupffer cells and the levels of nitrites and nitrates in the co culture supernatant increased over time , and this increase was attenuated either by addition of NO synthesis inhibitors , anti ICAM 1 or anti CD 18 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
METHOD : 15 limbal conjunctival specimens from 14 patients with the progressive Mooren ' s ulcer were examined by using immunohistochemical stainings for T lymphocyte and its subsets ( Pan T , TH and TS ) , B lymphocyte , intercellular adhesion molecule 1 ( ICAM 1 ) and lymphocyte function associated antigen 1 ( LFA 1 ) . ^^^ RESULT : The ratio of T helper to T suppressor cells ( TH / TS ) in Mooren ' s ulcer group was significantly higher than that in the normal group , ICAM 1 was strongly expressed on conjunctival cells , whereas LFA 1 was strongly expressed in infiltrating leukocytes . ^^^ Simultaneously , the mutual action of ICAM 1 and LFA 1 might play an important role in the development of the disease . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The resultant low density cell fraction consisted of a nonadherent cell population expressing different intensities of CD11c that could at least be characterized by typical DC morphology ( e . g . abundant cytoplasma with veil like cytoplasmatic dendrites , irregularly shaped nuclei , multivesicular and multilamellar bodies ) , constitutive levels of surface MHC class 2 , the presence of macrophage specific markers , such as F4 / 80 , Mac 1 and Fc receptors , respectively , on subpopulations of CD11c+ sorted cells and expression of adhesion and co stimulatory receptors like ICAM 1 and CD 44 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We have assessed the pattern of expression of the selectins ( E , P and L ) , and the counter receptors ( LFA 1 and ICAM 1 , and VLA 4 and VCAM 1 in 119 renal biopsies using sequential sections , and have correlated this with the degree of histological damage ( tubular atrophy and interstitial fibrosis ) and the intensity of the macrophage infiltrate . ^^^ There were strong correlations between the following : ( 1 ) expression of LFA 1 , VLA 4 , and L selectin in the periglomerular region , interstitium and in focal interstitial infiltrates and the presence of macrophages in these regions ; ( 2 ) de novo tubular expression of ICAM 1 and VCAM 1 ; ( 3 ) staining for ICAM 1 and VCAM 1 on focal cellular infiltrates within the interstitium ; and ( 4 ) staining for E and P selectin on extraglomerular endothelium . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
With the use of monoclonal antibodies against CD 18 and ICAM 1 , it was demonstrated that the increased adhesion was mainly mediated by the ICAM 1 / beta 2 integrin interaction . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Cell interaction molecules lymphocyte function associated antigen 1 ( LFA 1 ) ( CD11a ) , intracellular adhesion molecule 1 ( ICAM 1 ) ( CD 54 ) , ICAM 3 ( CD 50 ) and B7 . 2 ( CD 86 ) were up regulated on HL 60 R 7 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Lymphocytes were treated with antibodies specific for CD11a ( alpha L subunit of LFA 1 ) , CD 18 ( beta 2 subnit of the leucam family ) and CD 49 d ( alpha 4 subnit of very late activation antigen 4 , VLA 4 ) , and the RPE with antibodies specific for CD 54 ( intracellular adhesion molecule 1 , ICAM 1 ) and CD 106 ( vascular cell adhesion molecule 1 , VCAM 1 ) . ^^^ Role of LFA 1 , ICAM 1 , VLA 4 and VCAM 1 in lymphocyte migration across retinal pigment epithelial monolayers in vitro . ^^^ Migration across unstimulated RPE was inhibited by antibodies to ICAM 1 ( 48 . 6 + / 3 . 5 % reduction ) , leucocyte functional antigen 1 ( LFA 1 ) alpha ( 61 + / 5 . 2 % ) and LFA 1 beta ( 63 . 2 + / 4 . 7 % ) , but not by antibodies to VLA 4 . ^^^ Following activation of the RPE monolayers for 72 hr with IFN gamma , antibodies to LFA 1 alpha , LFA 1 beta and ICAM 1 inhibited migration by 49 . 9 + / 9 . 4 % , 63 . 6 + / 5 . 5 % and 47 . 7 + / 4 . 2 % respectively . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule 1 ( ICAM 1 , CD 54 ) , an adhesion molecule of the immunoglobulin superfamily , is an endothelial cell surface ligand for such leukocyte integrins as lymphocyte function associated molecule 1 ( LFA 1 , CD11a / CD18 ) , Mac 1 ( CD11b / CD18 ) and CD 43 . ^^^ We examined the expression of ICAM 1 in renal tissues of Masugi nephritis rats and directly examined the role of ICAM 1 by administration of neutralizing monoclonal antibodies ( MAbs ) to rat ICAM 1 , LFA 1 alpha subunit ( LFA 1 alpha ) , beta subunit ( LFA 1 beta ) and Mac 1 alpha subunit ( Mac 1 alpha ) . ^^^ Proteinuria was significantly suppressed by the MAbs against ICAM 1 , Mac 1 alpha and LFA 1 beta . ^^^ Neutrophil infiltration into the glomeruli was significantly prevented by injection of the MAbs against ICAM 1 , LFA 1 alpha and LFA 1 beta . ^^^ These results indicate that both ICAM 1 / LFA 1 and ICAM 1 / Mac 1 pathways are involved in neutrophil infiltration into the glomeruli . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Increased protection also occurred when mAb to E selectin was combined with mAb to either ICAM 1 or CD 18 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
An LFA 1 ICAM 1 mechanism of PMNL migration through HSF and HDF monolayers could be detected after treatment ( 4 hr ) of the monolayers with TNF alpha plus IFN gamma , which upregulated ICAM 1 on the fibroblasts . ^^^ The results demonstrate the beta 2 integrin dependent PMNL migration in connective tissue may involve primarily Mac 1 , with little involvement of LFA 1 or ICAM 1 , a situation in marked contrast to PMNL migration across endothelium . ^^^ However , in inflammatory conditions in which TNF alpha and / or IFN gamma may be generated , a role for LFA 1 ICAM 1 may be induced . . ^^^ Intercellular adhesion molecule 1 ( ICAM 1 ) was not required for Mac 1 mediated migration through HSF or HDF , because treatment of the fibroblasts with mAb R6 . 5 ( F ( ab ) 2 ) to ICAM 1 , which blocks the Mac 1 binding site on ICAM 1 , did not inhibit PMNL migration . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Although a short term blockade of intercellular adhesion molecule 1 ( ICAM 1 ) and leukocyte function associated antigen 1 ( LFA 1 ) adhesion allows induction of specific tolerance to solid organs , their effect on primary skin allografts is not yet known . ^^^ Effects of anti ICAM 1 and anti LFA 1 monoclonal antibodies ( mAb ) on primary skin allograft survival were investigated . ^^^ Recipient mice were treated with either a combination of anti ICAM 1 and anti LFA 1 mAbs or a control mAb for the first 22 days . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The cytotoxicity was inhibited by anti intercellular adhesion molecule 1 ( ICAM 1 ) / LFA 1 antibodies , but not by anti VCAM 1 / VLA 4 antibodies , both in vitro and in vivo . ^^^ In conclusion , Con A induced hepatitis is thought to be dominantly mediated by a perforin dependent pathway through ICAM 1 / LFA 1 interaction . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Localization of activated natural killer ( A NK ) cells in the microvasculature of growing tumors is the result of recognition of the intracellular and vascular cell adhesion molecules ICAM 1 and VCAM 1 on the tumor endothelium , mediated by lymphocyte function associated protein LFA 1 and vascular lymphocyte function associated protein VLA 4 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
OBJECTIVE : Because recruitment and retention of lymphoid cells appear to be critical components of the pathogenesis of lichen planus , we have compared the expression and distribution of a panel of vascular adhesion molecules ( ELAM 1 , P selectin , ICAM 1 , VCAM 1 , PECAM 1 , CD 34 ) and leukocyte adhesion molecule ligands ( LFA 1 , Mac 1 , VLA 4 , L selectin ) in biopsies of this disease . ^^^ Almost all cells in the lichen planus infiltrates stained positive for ICAM 1 , L selectin , LFA 1 , and VLA 4 , and large numbers of cells also exhibited VCAM 1 , PECAM 1 , and Mac 1 immunoreactivity . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The roles of HPMC ICAM 1 and the PMN counter receptor LFA 1 ( CD11a / CD18 ) in the adherence of PMN to HPMC were confirmed by using anti CAM Fab 2 ' fragments , and anti integrin antibodies , all of which significantly reduced the adherence of PMN to both control and cytokine treated HPMC . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
For LFA 1 , a similar method has previously been established using an ICAM 1 fusion protein . ^^^ We propose that the adhesiveness of the alpha 4 integrins for VCAM 1 and of LFA 1 for ICAM 1 is regulated by similar or identical protein kinases and phosphatases . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We investigated the expression of adhesion molecules including LFA 1 alpha ( CD11a ) , Mac 1 ( CD11b ) , LFA 1 beta ( CD 18 ) , VLA beta 1 ( CD 29 ) , H CAM ( CD 44 ) , VLA 4 ( CD49d ) , VLA 5 ( CD49e ) , ICAM 1 ( CD 54 ) , N CAM ( CD 56 ) , LFA 3 ( CD 58 ) , VNR beta ( CD 61 ) , and LECAM 1 ( CD62L ) on fresh myeloma cells and human myeloma cell lines . ^^^ These results suggest that VLA 4 , VLA 5 , ICAM 1 , LFA 3 , and H CAM are involved in cellular interaction and migration in MM , and that the expression of N CAM and LFA 1 varies with disease activity in MM . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
These experiments were carried out in the presence of neutralising antibodies to CD 18 , CD11b , LFA 1 , E selectin , P selectin , intracellular adhesion molecule 1 ( ICAM 1 ) , and ICAM 2 ; a novel CD11b / CD18 antagonist , neutrophil inhibitory factor ( rNIF ) ; adenosine receptor agonists ( 5 ' N ethycarboxamido adenosine / N6 cylopentyladenosine ( NECA / CPA ) ) and a platelet activating factor ( PAF ) receptor antagonist lexipafant . ^^^ Adhesion of stimulated neutrophils to resting monolayers was Mac 1 , CD 18 dependent and ICAM 1 , ICAM 2 , E selectin , P selectin , PAF independent . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To further examine the immune mechanisms which potentially are involved in alcoholic cirrhosis and the relationship to ethanol , we have determined the expression of surface antigens CD 4 , CD 8 , and of adhesion molecules CD 25 , LFA 1 , ICAM 1 and LFA 3 in patients and in response to stimulation with OKT 3 , IL 2 and with ethanol in vitro . ^^^ Lymphocytes from patients showed increased basal and stimulated expression of CD 4 , CD 25 , LFA 1 , ICAM 1 and LFA 3 molecules and increased TNF alpha production in comparison to controls . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Phosphatase inhibitors promote ICAM 1 and LFA 1 mediated homotypic aggregation of Jurkat and U 937 cells . ^^^ Monoclonal antibodies to LFA 1 completely inhibited the OA and CA induced aggregation in both cell lines , whereas the monoclonal antibody to ICAM 1 only inhibited U 937 cell aggregation . ^^^ These data suggest that phosphatase activity is important in the regulation of ICAM 1 and LFA 1 mediated homotypic aggregation in leukocytes . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We found up regulation of local cytokine genes ( IL 1 beta , TNF alpha , IL 2 , IFN gamma , IL 6 , IL 10 , IL 12p40 ) and cell adhesion molecule genes ( ICAM 1 , LFA 1 , CD 44 , Mel 14 ) in the salivary glands from mice with EAS by RT PCR , which were supported by immunohistochemistry . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
These findings suggest that ICAM 1 production is induced by endotoxins and cytokines produced in excess by inflammatory reactions and that endotoxins and cytokines are involved in qualitative , but not quantitative changes in LFA 1 ( CD11a / CD18 ) and Mac 1 ( CD11b / CD18 ) . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Intrathecal administration of antibodies against LFA 1 and against ICAM 1 suppresses experimental allergic encephalomyelitis in rats . ^^^ The effects of intracerebroventricular administration of mAbs against LFA 1 and ICAM 1 on both active and passive experimental allergic encephalomyelitis ( EAE ) in rats were examined . ^^^ LFA 1 specific mAbs and / or ICAM 1 specific mAbs or a control mAb or PBS were injected into the lateral ventricles via implanted needles . ^^^ In conclusion , intrathecal administration of antibodies against LFA 1 and ICAM 1 may be useful for the treatment of human demyelinating diseases , such as multiple sclerosis . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Cyclosporine A regulates T cell epithelial cell adhesion by altering LFA 1 and ICAM 1 expression . ^^^ We have examined the effect of CsA on the expression of the beta 2 integrin , LFA 1 , and its counter receptor , ICAM 1 , on a renal Ag specific murine T cell clone and Ag expressing renal tubular epithelial cells . ^^^ Although CsA does not inhibit ICAM 1 on T cells , it does inhibit surface expression of LFA 1 . ^^^ TGF beta 1 has similar effects on ICAM 1 and LFA 1 expression as high dose CsA , but the CsA effects are not mediated through induced TGF beta 1 expression . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Development of the mucosal immune system was examined in mice with partial loss of expression of ICAM 1 or CD 18 . ^^^ Normal expression of CD 18 integrins and ICAM 1 was essential for development of the CD 8 ( alpha beta ) TCR ( alpha beta ) LP and IEL compartment and for the generation of normal PP lymphocyte populations . ^^^ The presence of a subset of Thy1+TCR ( gamma delta ) IEL was also dependent on CD 18 integrins and ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Double immunofluorescence and indirect immunoperoxidase techniques were applied to muscle biopsies to define T cell phenotype and LFA 1 / ICAM 1 expression . ^^^ It is proposed that in PM , in contrast to DM , LFA 1 / ICAM 1 interactions enable activated CD8+ T cells to migrate selectively into the inflamed muscle and to adhere to myofibres , leading to tissue injury . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Ligation of the T cell receptor modulated the phenotypic expression of LFA 1 and ICAM 1 on both HA1 . 7 and DE 9 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
FK 506 treatment suppressed the accumulation of ED 1 positive cells , CD 4 positive cells , CD 8 positive cells , interleukin 2 ( IL 2 ) receptor positive cells , leukocyte function associated antigen 1 ( LFA 1 ) positive cells and intercellular adhesion molecule 1 ( ICAM 1 ) expression in nephritic glomeruli . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Anti intercellular adhesion molecule 1 ( anti ICAM 1 ) and anti lymphocyte function associated antigen 1 ( anti LFA 1 ) monoclonal antibodies ( mAbs ) were injected into mice and their effects on tumor metastasis were investigated using two murine models . ^^^ Depending on the dose , the anti ICAM 1 mAb ( KAT 1 ) expressed both inhibitory and promoting effects on liver metastases of ICAM 1 + LFA 1 + P 815 mastocytoma cells , whereas it enhanced lung metastases of the ICAM 1 LFA 1 Meth A fibrosarcoma cells at any doses . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Strategies targeting lymphocyte function associated antigen 1 ( LFA 1 , CD11a / CD18 ) and intercellular adhesion molecule 1 ( ICAM 1 ) have previously been shown to produce long term survival of solid organ allografts in animals only when both CD11a and ICAM 1 are targeted for a brief ( 6 7 days ) time or when extended ( 14 weeks ) treatment with anti CD11a monoclonal antibody ( mAb ) is administered . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Although differences between specific autoimmune diseases exist , key interactions facilitating the development of autoimmune inflammation appear to include L selectin / P selectin / E selectin , lymphocyte function associated antigen 1 ( LFA 1 ) / intercellular adhesion molecule 1 ( ICAM 1 ) , very late antigen 4 ( VLA 4 ) / vascular cell adhesion molecule 1 ( VCAM 1 ) , and alpha 4B7 / MadCAM or VCAM 1 adhesion . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
CD 2 , LFA 1 , and CD 28 are the major costimulatory and adhesive molecules on T cells and bind to the LFA 3 , ICAM 1 and B 7 ligands , respectively , on APC . ^^^ The LFA 1 / ICAM 1 pathway stimulates adhesion and cell proliferation more efficiently in memory T helper cells than in naive cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The lymphocyte function associated antigen ( LFA 1 ) / intercellular adhesion molecule 1 ( ICAM 1 ) pathway has been shown to be involved in the homing of lymphocytes to arthritic joints in animal models . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
For immunohistochemistry , monoclonal antibodies directed against CD 3 , CD 4 , CD 8 , CD10a , CD 36 , and HLA DR antigens , were used , as well as antibodies directed against adhesion molecules ICAM 1 , ELAM 1 and LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Blocking antibodies against the adhesion molecule ICAM 1 and leukocyte counterreceptors ( LFA 1 , VLA 4 ) prevented the proliferative response , which could not be duplicated with the conditioned media of U 937 alone or co cultured with mesangial cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Activated polymorphonuclear leukocytes induce constriction of canine coronary artery via Mac 1 , but not LFA 1 , and ICAM 1 . ^^^ The PMN induced vasoconstriction ( 5 10 10 ( 6 ) cells / ml : 98 + / 8 % of KCl induced constriction ) was inhibited when PMNs were pretreated with the monoclonal antibody against Mac 1 ( 54 + / 4 % of KCl induced constriction ) but not against LFA 1 against ICAM 1 ( 31 + / 2 % of KCl induced constriction ) . ^^^ These results suggest that activated PMNs may mediate adherent dependent constriction of canine coronary artery via Mac 1 ( CD11b / CD18 ) , but not LFA 1 ( CD11a / CD18 ) , with ICAM 1 . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Here we review data ( a ) on the expression of some costimulatory ( MHC class 2 , CD 80 , sialoadhesin ) and adhesion ( LFA 1 , ICAM 1 , VLA 4 ) molecules on both metastasized tumor cells and host cells and ( b ) on the production of a cytotoxic molecule , nitric oxide , by in situ activated Kupffer and endothelial cells in the process of liver metastasis . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
This study examined the effects of hypercarbic acidosis ( 10 or 20 % CO 2 ) and of hypocarbic alkalosis ( 0 % CO 2 ) on human neutrophil CD 18 and human aortic endothelial cell intercellular adhesion molecule 1 ( ICAM 1 ) , vascular cell adhesion molecule 1 ( VCAM 1 ) , and E selectin expression quantified by flow cytometry . ^^^ The results suggest that acidosis generated during tissue ischemia / inflammation may induce CD 18 mediated neutrophil adhesion despite a decrease in ICAM 1 expression . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Blocking of ICAM 1 , LFA 1 , VCAM 1 , E selectin , and P selectin did not reduce TNF enhanced metastasis even though tumor cell adhesion in vitro was reduced . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In a skin delayed type hypersensitivity model analyzed for comparison , mAbs against LFA 1 / ICAM 1 and alpha 4 integrin showed the largest effects on ear swelling . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To investigate the mechanism of synovial pannus formation in rheumatoid arthritis , immunohistochemical and immunoelectron microscopic studies with monoclonal antibodies against the adhesion molecules , CD 54 ( ICAM 1 ) , CD11a ( LFA 1 ) , CDw49a ( VLA 1 ) , CDw49b ( VLA 2 ) , CDw49c ( VLA 3 ) , Cdw49d ( VLA 4 ) and CDw49e ( VLA 5 ) , were carried out to determine the pattern of distribution of these molecules at the rheumatoid synovial cartilage junction . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Distribution of immunoglobulin superfamily members ICAM 1 , 2 , 3 , and the beta 2 integrin LFA 1 in multiple sclerosis lesions . ^^^ These results suggest a role for ICAM 1 , 2 , and LFA 1 in the transendothelial migration of leukocytes into MS brain and a role for ICAM 3 / LFA 1 interactions in the activation of lymphocytes , monocytes , and microglia in MS lesions . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In vivo effects of monoclonal antibody against ICAM 1 and LFA 1 on antigen induced nasal symptoms and eosinophilia in sensitized rats . ^^^ We applied anti ICAM 1 and anti LFA 1 monoclonal antibody ( mAb ) to ovalbumin sensitized rats and examined the effects on nasal eosinophilia and nasal symptoms following topical antigen challenge . ^^^ These findings suggest that the ICAM 1 / LFA 1 system is important in topical allergic inflammation in rats . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
T cells infiltrating the CNS are recently activated / memory lymphocytes typified by their high expression of CD 44 , LFA 1 and ICAM 1 and low expression of CD45RB in the mouse . ^^^ Following the induction of EAE in susceptible mice , ICAM 1 and VCAM 1 are dramatically upregulated on CNS vessels ; lymphocytes bind to such vessels via the interaction of their known ligands , LFA 1 / Mac 1 and alpha 4 integrins , at least in vitro . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The expression of the intercellular adhesion molecule 1 ( ICAM 1 ) and its ligand lymphocyte function associated antigen 1 ( LFA 1 or alpha L ) , the vascular cell adhesion molecule 1 ( VCAM 1 ) , endothelial leukocyte adhesion molecule 1 ( ELAM 1 ) , and the cellular receptors for extracellular matrix , alpha 1 , alpha 2 , alpha 3 , alpha 5 , alpha 6 , alpha 5 , beta 1 , and beta 3 integrin subunits , was studied in 28 patients with crescentic glomerulonephritis ( GN ) related to several mechanisms : four patients with anti glomerular basement membrane antibodies or anti GBM disease ; 16 with immune complex mediated GN ; and eight with pauci immune GN , associated with vasculitis in four cases . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Immunohistochemistry was performed for CD 18 , CD11a , CD11b , CD11c , alpha d , ICAM 1 , ICAM 2 , and ICAM 3 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
METHODS : The expression of the integrin VLA beta 1 , alpha subunits of the beta 2 integrins LFA 1 , Mac 1 , and p 150 , 95 , the members of the immunoglobulin family ICAM 1 and VCAM 1 , and the selectin ELAM 1 on corneas with bullous keratopathy ( BK ) secondary to intraocular surgery was studied immunohistochemically using an APAAP method . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The present study demonstrated that a short term administration of mAbs against leukocyte function associated antigen 1 ( LFA 1 ) and intercellular adhesion molecule 1 ( ICAM 1 ) at critical periods resulted in complete protection of autoimmune diabetes in non obese diabetic ( NOD ) mice . ^^^ It appears that the tolerance against beta cell Ag ( s ) was induced by this transient blockade of the LFA 1 / ICAM 1 pathway . ^^^ These results suggest that a unique peripheral tolerance was induced by the transient blockade of the LFA 1 / ICAM 1 pathway in an early age of NOD mice . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
ICAM 1 , 2 and LFA 1 independent homotypic T cell aggregation induced by a novel activating monoclonal antibody targeting the murine Thy 1 molecule . ^^^ Thy 1 induced HTA can not be blocked with MAb targeting intercellular adhesion molecule 1 and 2 ( ICAM 1 , ICAM 2 ) or LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Adherence of peripheral blood mononuclear cells ( PBMC ) and cytotoxicity mediated by adherent activated T cells were blocked by treatment of CMV infected fibroblasts with anti ICAM 1 antibody and by treatment of leukocytes with anti LFA 1 antibody . ^^^ These data suggest that an interaction of ICAM 1 and LFA 1 is responsible for the adherence of leukocytes and for adherent activated T cell mediated cytotoxicity against CMV infected fibroblasts . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
E . coli enhanced the expression of intercellular adhesion molecule 1 ( ICAM 1 ) on urinary tract epithelial cells , and neutrophil migration across urinary tract epithelial layers in vitro was dependent on epithelial ICAM 1 and neutrophil Mac 1 ( CD11b / CD18 ) expression . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Antibodies against both ICAM 1 and LFA 1 partially inhibited the chemotactic activity , but this was not the case with anti TNF alpha antibody , anti GM CSF antibody , fibronectin RGDS . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Aggregation was independent of intercellular adhesion molecule 1 ( ICAM 1 ) / lymphocyte function associated antigen 1 ( LFA 1 ) , but could be inhibited by monoclonal antibodies ( mAb ) against the MV hemagglutinin glycoprotein ( MV H ) . mAb against the MV receptor , CD 46 , also blocked aggregation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Infection of A 549 cells with a recombinant adenovirus vector induces ICAM 1 expression and increased CD 18 dependent adhesion of activated neutrophils . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
By both immunohistochemistry and lymphocyte adhesion assays , the predominant interaction identified was between intercellular adhesion molecule 1 ( ICAM 1 ) and its cognate integrin , leukocyte functional antigen 1 ( LFA 1 ) . ^^^ A role for ICAM 1 in recruiting lymphocytes to the hepatic granuloma was also supported by the observation that lymphocytes preincubated with anti LFA 1 antibody did not bind to granulomas in tissue sections . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
FDCs were immunochemically positive for Mac 1 ( CD11b ) , sialyl Lex ( CD15s ) , CD 22 , integrin beta 1 ( CD 29 ) , CD 40 , very late activation antigen ( VLA ) alpha 3 ( CD49c ) , VLA alpha 5 ( CD49e ) , VLA alpha 6 ( CD49f ) , intercellular adhesion molecule ( ICAM ) 3 ( CD 50 ) , ICAM 1 ( CD 54 ) , B 7 ( CD 80 ) , and vascular cell adhesion molecule ( VCAM ) 1 ( CD 106 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Role of ICAM 1 and LFA 1 in endotoxin induced uveitis in mice . ^^^ Monoclonal anti ICAM 1 antibody or anti LFA 1 antibody was intraperitoneally administered to C3H / HeN mice simultaneously with endotoxin , and the effect on the development of EIU was studied . ^^^ Moreover , there were no differences between these two antibodies in the effect on EIU , whereas anti LFA 1 antibody was more effective than anti ICAM 1 antibody in IL 1 enhanced EIU . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Immunosuppression by anti ICAM 1 and anti LFA 1 monoclonal antibodies of free and vascularized skin allograft rejection . ^^^ Our results suggest that interaction with ICAM 1 and LFA 1 is more important in the rejection of vascularized skin allografts than that of free skin allografts . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Monocyte derived macrophages prime peripheral T cells to undergo apoptosis by cell cell contact via ICAM 1 / LFA 1 dependent mechanism . ^^^ The addition of blocking antibodies against LFA 1 , ICAM 1 and CD 2 to the cocultures significantly inhibited the SEB induced T cell apoptosis . ^^^ We concluded therefore that direct contact of macrophages with T cells is critical in SEB induced T cell apoptosis , and that adhesion molecules such as LFA 1 / ICAM 1 and CD 2 may be involved in the mechanism of this effect . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Integrin CR 3 ( CD11b / CD18 , Mac 1 , alpha M beta 2 ) mediates the transient adhesion of polymorphonuclear leukocytes ( PMN ) to surfaces coated with fibrinogen , C3bi , ICAM 1 , and other ligands . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The adhesion between FDCs and B cells is mediated by ICAM 1 ( CD 54 ) LFA 1 ( CD11a ) and VCAM VLA 4 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Frozen brain sections were prepared and stained with monoclonal antibodies to complement receptor type 3 ( OX 42 ) , major histocompatibility complex ( MHC ) class 1 and class 2 antigens ( OX 18 and OX 6 , respectively ) , a pan macrophage / monocyte marker ( ED 1 ) , intercellular adhesion molecule 1 ( ICAM 1 ) , LFA 1 alpha chain ( CD11a ) and beta chain ( CD 18 ) , and T cells ( CD 5 ) . ^^^ The invading leukocytes expressed CD 11 and CD 18 adhesion molecules on their cell surface while ICAM 1 was expressed on endothelial cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Fibre samples were processed for scanning electron microscopy ( SEM ) , or for immunofluorescence labelling of the MAC 1 and ICAM 1 cell surface markers . ^^^ Confocal microscopy demonstrated a difference between materials in the number of MAC 1 and ICAM 1 positive cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
By flow cytometry , it could be shown that among several surface adhesion molecules ( ICAM 1 / CD54 , VLA 4 / CD49d , Mac 1 / CD11b , LFA 1 / CD11a , LFA 2 / CD2 , LFA 3 / CD58 , VCAM 1 ) , only the constitutively expressed immunoglobulin family member intercellular adhesion molecule 1 ( ICAM 1 ) is modulated by proinflammatory cytokines on HMC 1 mast cells . ^^^ This cytokine induced upregulation of ICAM 1 expression might reveal a putative regulatory mechanism of mast cell interaction with effector cells bearing the counterparts of ICAM 1 ( CD 54 ) , the molecules Mac 1 ( CD11b / CD18 ) and leukosialin ( CD 43 ) , and the principal ligand LFA 1 ( CD11a / CD18 ) . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
There were no significant differences , however , in the expression of CD 23 , CD 32 , Mac 1 and adhesion molecules ( ICAM 1 , leukocyte function associated antigen 1 alpha ( LFA 1 alpha ) , LFA 1 beta , very late antigen 4 ) on eosinophils of patients with strongyloidiasis positive for anti HTLV 1 antibodies in comparison with those negative for these antibodies . . ^^^ The expression of CD 23 , Mac 1 and intercellular adhesion molecule 1 ( ICAM 1 ) on eosinophils of patients with strongyloidiasis was augmented in comparison with normal subjects and HTLV 1 carriers . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
LFA 1 and ICAM 1 are crucial for the induction of hyperreactivity in the mouse airways . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The expression of intracellular adhesion molecule 1 ( ICAM 1 ) ( CD 54 ) and lymphocyte function associated antigen 1 ( LFA 1 ) ( CD11a / CD18 ) are important for the interaction of activated leukocytes with the brain . ^^^ Therefore , the expression of ICAM 1 in the cerebral vessels and LFA 1 on leukocytes were examined after reperfusion in a rat four vessel occlusion model . ^^^ Immunohistological staining for ICAM 1 and LFA 1 was performed in each subgroup . ^^^ Increased expression of ICAM 1 and LFA 1 were induced by reperfusion , and this may be important in reperfusion injury of the brain . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
A major factor in cellular cytotoxicity is the interaction between LFA 1 on leukocytes and ICAM 1 on targets . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Application to ICAM 1 / LFA 1 mediated cellular adhesion . ^^^ The binding of lymphocyte function associated antigen ( LFA 1 ) to intercellular adhesion molecule ( ICAM 1 ) is responsible for several types of cellular adhesion . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The human lymphatic leukemia cell line HPB ALL was chemically mutagenized and rare cells expressing constitutively active beta 2 integrin LFA 1 ( alpha L beta 2 , CD11a / CD18 ) were selected from the pool of mutagenized cells , by repeated cycles of binding to immobilized ICAM 1 . ^^^ In contrast to the original cell line , HAP 4 had constitutively active LFA 1 and adherence to ICAM 1 was insensitive to inhibition by okadaic acid and cytochalasin B or to inhibition of ATP synthesis . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Most of these cells also expressed HLA DR and LFA 1 and ICAM 1 antigens . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
A similar effect was observed by pretreatment of the effector ( LAK ) cells with antibodies directed against LFA 1 , the receptor for ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Moreover , they express a high level of adhesion molecules such as Lfa 1 , Icam 1 and Lpam 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To help define the role of adhesion molecules in HAM / TSP , we studied the expression of lymphocyte function associated antigen 1 ( LFA 1 ) , Mac 1 , very late antigen 4 ( VLA 4 ) , Sialyl Lewisx ( SLex ) , intercellular adhesion molecule 1 ( ICAM 1 ) , vascular cell adhesion molecule 1 ( VCAM 1 ) , endothelial leukocyte adhesion molecule 1 ( ELAM 1 ) and monocyte chemoattractant protein 1 ( MCP 1 ) in the spinal cord lesions of HAM / TSP . ^^^ Although the expression of ICAM 1 in the spinal cords was not distinctive between HAM / TSP and controls , infiltrating mononuclear cells in the spinal cords of HAM / TSP strongly expressed LFA 1 and Mac 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The autologous MECLR was inhibited in samples from all patients by mAb to CD 2 ( LFA 2 ) , CD11a ( LFA 1alpha ) , CD 18 ( LFA 1beta ) , ICAM 1 , CD 58 ( LFA 3 ) and an antiserum to IL 1beta . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The appearance of cytokine producing cells in the hearts correlated with an increased local expression of class 1 and class 2 MHC molecules and adhesion molecules ( ICAM 1 , LFA 1 , VLA 4 , and VCAM 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Differential expression of ICAM 1 and LFA 1 versus L selectin and VCAM 1 in autoimmune insulitis of NOD mice and association with both Th 1 and Th 2 type infiltrates . ^^^ NOD mouse islets did not show the expression of ICAM 1 , LFA 1 , L selectin and VCAM 1 prior to infiltration by mononuclear cells . ^^^ ICAM 1 and LFA 1 were first demonstrable in islets with strong periinsular infiltrates ( insulitis grade 2 ) while L selectin and VCAM 1 were only seen in islets with mild or strong intraislet infiltration ( grade 3 4 ) . ^^^ ICAM 1 and LFA 1 expression is seen prior to L selectin and VCAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The interaction of LFA 1 on T lymphocytes with ICAM 1 on antigen presenting cells ( APCs ) is critical in determining conjugate formation between the APCs and T cells as well as activation of T cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies ( 2 mg / kg ) to ICAM 1 and the alpha chain ( CD11a , CD11b ) or the beta chain ( CD 18 ) of beta 2 integrins had no significant effect on hepatic neutrophil count after endotoxin . ^^^ In contrast , these antibodies inhibited peritoneal neutrophil infiltration in response to glycogen administration by 28 % ( CD11b ) , 60 % ( CD11a , ICAM 1 ) , and 92 % ( CD 18 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The expression of LFA 1 was higher on all leucocyte subsets of the marginal pool except the granulocytes , whereas no differences were seen for the expression of other adhesion molecules ( alpha 4 integrins , ICAM 1 , CD 2 , L selectin , and CD 44 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To investigate the mechanisms of mononuclear cell infiltration , we examined the expression of the intercellular adhesion molecule 1 ( ICAM 1 ) and its counterpart lymphocyte function associated antigen 1 ( LFA 1 ) in the progression of hypertensive renal injury . ^^^ Increased ICAM 1 was associated with an accumulation of LFA 1 positive mononuclear cells in the perivascular region ( day 14 : 15 + / 4 versus 2 + / 0 . 2 cells / mm2 in sham , P < . 005 ) and intertubular region ( 127 + / 11 versus 32 + / 3 cells per millimeter squared in sham , P < . 005 ) . ^^^ In addition , the number of interstitial LFA 1 positive infiltrating cells was related to the degree of interstitial and tubular ICAM 1 expression and correlated with blood pressure ( r = . 75 , P < . 001 , n = 18 ) . ^^^ Our data suggest that ICAM 1 is involved in the recruitment of macrophages / lymphocytes via specific interaction of ICAM 1 and LFA 1 in this model of hypertensive target organ damage . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
A peptide isolated by phage display binds to ICAM 1 and inhibits binding to LFA 1 . ^^^ This is the region of the ICAM 1 that is bound by LFA 1 . ^^^ The phage bound to two ICAM 1 mutants , which contained amino acid substitutions that dramatically decreased or eliminated the binding to LFA 1 . ^^^ The linear form of the synthetic EWCEYLGGYLRCYA peptide was found to inhibit LFA 1 binding to immobilized ICAM 1 ( 1 453 ) in a protein protein binding assay . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Among the six adhesion molecules examined ( LFA 1 , ICAM 1 , CLA , VLA 4 , L selectin and CD 44 ) , L selectin increased the incidence of lymph node metastasis , and CD 44 expression was related to both lymph node and organ ( hematogenous ) metastasis . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Addition of TMB 8 , a calcium inhibitor , or monoclonal antibody directed against ICAM 1 or CD 18 prevented the increases in fluo 3 fluorescence and NO production of Kupffer cells and Kupffer cell induced mitochondrial dysfunction in AH 70 cells , suggesting the involvement of calcium mobilization and CD18 / ICAM 1 . ^^^ The NO production and calcium mobilization of Kupffer cells and reduction of the mitochondrial membrane potential in cocultured hepatoma cells were diminished in the case of Kupffer cells isolated from chronically ethanol fed rats , while CD 18 and ICAM 1 expression was still observed . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The sections were also stained immunohistochemically for the vascular endothelial adhesion molecules ICAM 1 , ELAM 1 ( E selectin ) , and VCAM 1 , and for the leukocyte ligands for ICAM 1 : LFA 1 ( CD11a / CD18 ) and Mac 1 ( CD11b / CD18 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Evidence that the LFA 1 binding sites for ICAM 1 , 2 , and 3 are distinct . ^^^ The same was true for adhesion of LFA 1 to ICAM 1 . ^^^ As previously described , mouse LFA 1 does not bind to human ICAM 1 but we show here that mouse LFA 1 does bind to human ICAM 2 and 3 . ^^^ These results indicate that the LFA 1 binding sites for ICAM 1 and ICAM 2 and 3 , although in close vicinity , are distinct . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Four different combinations of monoclonal antibodies against rat ICAM 1 , CD 11a , and CD 18 were utilized to determine the relative importance of LFA 1 , Mac 1 , and ICAM 1 in a rat model of severe lung allograft reperfusion injury . ^^^ We found that the combination of anti ICAM 1 ( 1 mg / kg ) added to the donor lung flush , and anti CD11a , anti CD 18 , and anti ICAM 1 ( 2 mg / kg i . v . of each ) given to the recipient prior to reperfusion , resulted in significantly improved lung graft pAO 2 by ABG , and decreased alveolar protein , cell count , and myeloperoxidase activity compared with control animals . ^^^ We conclude that LFA 1 , Mac 1 , and ICAM 1 are all important adhesion molecules in lung allograft reperfusion injury yet even with antibody blockade of all three there are additional mechanisms allowing for neutrophil influx into the lungs . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In this study , the expression of vascular adhesion molecules ICAM 1 , VCAM 1 and ELAM 1 in the liver allografts , the number of leukocytes positive for their ligands LFA 1 , VLA 4 and SLex , and activation markers ( class 2 , IL 2 receptor ) were investigated in liver allografts during CMV infection and acute rejection and compared to grafts with normal function and histology . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
T cell mediated immunity to lymphocytic choriomeningitis virus in beta 2 integrin ( CD 18 ) and ICAM 1 ( CD 54 ) deficient mice . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The aims of this study were to measure the immunosuppressive ability of castanospermine ( CAST ) in a rat heart allograft model , to measure its effect on membrane expression of adhesion molecules ( LFA 1 alpha , LFA 1 beta , ICAM 1 ) , class 1 and class 2 MHC antigens and on other T cell associated molecules ( CD 4 , CD 8 , CD 39 , CD 45 , W3 / 13 ) , to test its tolerogenic potential and its toxicity . ^^^ By contrast , in non grafted rats CAST significantly upregulated expression of class 1 MHC and CD 45 in the thymus , lymph node and spleen , ICAM 1 and CD 4 on lymphoid cells in the spleen , but reduced expression of LFA 1 alpha on lymphoid cells in the thymus . ^^^ In conclusion , CAST is an immunosuppressive molecule which may work by downregulation of the ligand receptor adhesion molecule pair , LFA 1 alpha ICAM 1 although subtle downregulation of class 1 and 2 MHC , CD 4 and CD 8 molecules could also contribute to its immunosuppressive activity . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The expression of CAM ( CD 18 , ICAM 1 ) appears important for the initiation of otitis media . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Substance P promotes lymphocyte endothelial cell adhesion preferentially via LFA 1 / ICAM 1 interactions . ^^^ The increased adhesiveness was associated with enhanced expression of ICAM 1 on endothelial cells and increased avidity of LFA 1 on lymphocytes . ^^^ These data suggest that SP modulates lymphocyte endothelial cell interactions by preferentially upregulating LFA 1 and ICAM 1 interactions . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The up regulation of intercellular adhesion molecule 1 ( ICAM 1 ) expression and increase in leukocyte function associated antigen 1 ( LFA 1 ) positive cells in nephritic glomeruli significantly declined with butein treatment . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Functional studies with the parental cell lines , the J ( beta 2 ) . 7 and SK ( beta 2 ) . 7 cells , and the transfectants showed that all binding of Jurkat and SKW 3 cells to purified ICAM 1 is mediated by LFA 1 , and the reconstituted LFA 1 expressed by the J ( beta 2 ) . 7 and SK ( beta 2 ) . 7 transfected cells is regulated normally . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Neutrophil CD 18 dependent arrest on intercellular adhesion molecule 1 ( ICAM 1 ) in shear flow can be activated through L selectin . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We focused our attention on ICAM 1 because it is the natural ligand of leukocyte functional associated antigen 1 and Mac 1 , expressed on eosinophils . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
These results indicate that LFA 1 ( CD11a / CD18 ) , Mac 1 ( CD11b / CD18 ) , and ICAM 1 ( CD 54 ) adhesion molecules are involved in natural killer ( NK ) cell induced DNA fragmentation in CMV infected cells . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
CT treatment of LB cells did not alter the expression of class 2 MHC molecules , CTLA 4 counter receptors , LFA 1 or ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Pretreatment with a monoclonal antibody directed against either CD 18 or intercellular adhesion molecule 1 ( ICAM 1 ) significantly prevented the ethanol induced increase in leukocyte adherence and decrease in barrier function of endothelium . ^^^ These results suggest that ethanol induced leukocyte adherence is mediated by CD 18 on leukocytes and ICAM 1 on endothelial cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies ( mAb ) against CD 18 , CD11b , and ICAM 1 inhibited PMN EC adhesion . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
With respect to other ligands , mAbs 1G12 and 2D5 completely inhibited attachment of Plasmodium falciparum infected erythrocytes to immobilized recombinant ICAM 1 Fc , whereas they had no effect on LFA 1 dependent T cell binding to ICAM 1 Fc . ^^^ Conversely , mAbs 3D6 and 6E6 completely abolished LFA 1 binding to ICAM 1 Fc . ^^^ By recognizing a site distinct from that of beta 2 integrins Mac 1 or LFA 1 , fibrinogen binding to ICAM 1 may provide an alternative pathway of intercellular adhesion and / or modulate integrin dependent adherence during inflammation and vascular injury . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Furthermore , we observed lymphocyte function associated antigen 1 ( LFA 1 ) mediated adhesion of HL 60 cells on recombinant TLN protein , as well as on ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Combined treatment with anti leukocyte function associated antigen 1 ( LFA 1 ) and anti intercellular adhesion molecule 1 ( ICAM 1 ) monoclonal antibodies leads to allograft tolerance in murine cardiac transplantation . ^^^ When heart and skin grafts from the same donor ( BALB / c [ H2d ] ) were simultaneously transplanted to C3H mice ( H2k ) , both were rejected within 29 days , even though the mice were similarly treated with anti LFA 1 and anti ICAM 1 monoclonal antibodies . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In the normal conjunctiva , intercellular adhesion molecule 1 ( ICAM 1 ) was expressed only on the vascular endothelium , lymphocyte function associated antigen 1 ( LFA 1 ) and intercellular adhesion molecule 3 ( ICAM 3 ) on epithelial and stromal mononuclear cells , and very late activation antigen 4 ( VLA 4 ) on a few stromal mononuclear cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The MM 46 cells expressed ICAM 1 while the PMNs expressed both ICAM 1 and LFA 1 as determined by immunohistochemical staining and immunoelectron microscopy using anti ICAM 1 and anti LFA 1 antibodies . ^^^ Immunoelectron microscopic findings suggested that the adhesion molecules were LFA 1 on the PMNs and ICAM 1 on the MM 46 tumor cells , but cell fusion between the PMNs and tumor cells was not observed . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
This study tested the hypothesis that prolonged consumption of alcohol directly or indirectly , through endotoxin influx in the circulation , stimulates the Kupffer cells to produce macrophage inflammatory protein 2 ( MIP 2 ) and up regulates the expression of adhesion molecules , i . e . , CD 18 on PMNs and its counter receptor , intercellular adhesion molecule 1 ( ICAM 1 ) , on hepatic cells . ^^^ At the same time , the expression of CD 18 and ICAM 1 on polymorphonuclear neutrophils ( PMNs ) and hepatic cells was increased more than twofold . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Cross linking of B cell immunoglobulin ( Ig ) receptors by antigen , stimulation of the integrin adhesion molecules LFA 1 and VLA 4 on the B cell through interaction with their counter receptors ICAM 1 and VCAM 1 on the FDC and cross linking of CD 40 on the B cells through interaction with the CD 40 ligand ( CD40L ) on T cells have been shown to prevent entry into apoptosis of GC B cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
These sIg+ GC B cells co express B 7 2 , ICAM 1 , and LFA 1 , and have potent antigen presenting activity which results in T cell activation in vitro . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Here we report that tepoxalin selectively inhibits intercellular adhesion molecule 1 ( ICAM 1 , CD 54 ) / MAC 1 ( CD11b / CD18 ) dependent adhesion of polymorphonuclear cells to IL 1 activated human umbilical vein endothelial cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
MoAb binding to adhesion molecules intercellular adhesion molecule 1 ( ICAM 1 ) and lymphocyte function associated antigen 1 ( LFA 1 ) also inhibited C . neoformans induced cell proliferation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The expression of the intercellular adhesion molecule 1 ( ICAM 1 ) , and the integrins CD 49 , CD11b / c , and CD11a ( LFA 1 alpha chain ) was analyzed in an experimental model of pulmonary fibrosis . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
They are classified in four families , each having an individual interest : antibody directed against the CD3 / T receptor complex ; anti interleukine 2 receptor antibodies , specifically blocking activated T lymphocytes ; antibodies against adhesion molecules , mainly LFA 1 and ICAM 1 , that , by preventing the reperfusion injury syndrome , should have many clinical applications in the future ; antibodies against co receptor molecules , such as CD 4 , inducing indefinite allograft tolerance in models of transplantation in rodents , that could be reproducible in human . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To analyze these migratory mechanisms of immune cells , we immunohistochemically examined the expression of the following : cellular adhesion molecules , lymphocyte function associated antigen 1 ( LFA 1 ) very late activation antigen 4 ( VLA 4 ) , and the alpha 5 ( alpha 5 ) subunit and their respective ligands , intercellular adhesion molecule 1 ( ICAM 1 ) , vascular cell adhesion molecule 1 ( VCAM 1 ) , and fibronectine ; the examination was carried out in pulmonary tissue from days 0 , 2 , 6 and 12 following intratracheal administration of sheep red blood cells ( SRBC ) as an antigen to previously sensitized mice . ^^^ The blood vessel endothelium was also stained positive for VCAM 1 and ICAM 1 , while many of the infiltrating lymphocytes were positive for VLA 4 and LFA 1 immunolabelings . ^^^ VLA 4 bearing lymphocytes conspicuously increased in number among the perivascular leukocytes , while immunoreactivity for LFA 1 , VCAM 1 , ICAM 1 and fibronectin was unchanged from that for post challenge day 2 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We investigated the effects of the antibodies against the adhesion molecules leucocyte function associated antigen 1 ( LFA 1 ) and intercellular adhesion molecule 1 ( ICAM 1 ) on mouse allergic contact hypersensitivity . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Sections were stained with specific antibodies against ( 1 ) L selectin and ( 2 ) LFA 1 on leukocytes , ( 3 ) ICAM 1 on endothelial cells , ( 4 ) PMNs , and ( 5 ) monocytes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
A photobleaching energy transfer analysis of CD8 / MHC 1 and LFA 1 / ICAM 1 interactions in CTL target cell conjugates . ^^^ Two examples , as possible application areas of this approach , are presented here : interaction between CD 8 and MHC 1 molecules in point contacts and interaction between LFA 1 and ICAM 1 molecules in focal contacts of CTL target conjugates . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Human intercellular adhesion molecule 1 ( ICAM 1 ) plays an important role in immune responses as the major specific ligand for the beta 2 integrins LFA 1 and Mac 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Particularly , we focused our attention on ICAM 1 expression on epithelial cells , since it is the natural ligand of LFA 1 , an adhesion molecule expressed by leucocytes , including eosinophils . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
A flow cytometric analysis revealed that SN12C MM 3 showed a strong expression of sialyl Lewis 10 ( sialyl Le ( 10 ) ) carbohydrate antigen and a slightly stronger expression of CD11a ( LFA 1 alpha chain ) and CD 54 ( ICAM 1 ) compared with the other three cell lines . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Blocking experiments utilizing monoclonal antibodies specific to various cell adhesion molecules revealed that very late antigen ( VLA ) integrins , lymphocyte function associated antigen ( LFA 1 ) / intercellular adhesion molecule 1 ( ICAM 1 ) , CD44 / hyarulonate are , at least in part , involved in lymphocyte fibroblast interactions . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In this study , we investigated the involvement of the adhesion molecules intercellular adhesion molecule 1 ( ICAM 1 ) , vascular cell adhesion molecule 1 ( VCAM 1 ) , very late activation antigen 4 ( VLA 4 ) , lymphocyte function associated antigen 1 ( LFA 1 ) and macrophage antigen 1 ( Mac 1 ) on eosinophil infiltration in the cutaneous late phase response ( LPR ) in OVA sensitized Balb / c mice by two approaches , immunostaining and inhibition assays with each monoclonal antibody . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
On the other hand , it has been reported that AH66F cells are unique in having leukocyte function associated antigen 1 ( LFA 1 ) on the outer cell membrane , adhering through interaction with intercellular adhesion molecule 1 ( ICAM 1 ) on M cells , although AH 130 cells are not so efficient as other hepatoma cells . ^^^ Consequently , the adhesion of AH66F cells to M cells was inhibited by anti LFA 1 beta chain monoclonal antibody and anti ICAM 1 antibody . ^^^ When cells were cultured separately with FCS or ASF , and the adhesion molecules were analysed using flow cytometry , the expression of LFA 1 molecules on AH66F cells was not changed by eitherv FCS or ASF , but the ICAM 1 molecule on M cells was increased time dependently by ASF . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The expression of a series of adhesion receptors : L selectins ( CD62L ) : Leu 8 , several integrins ( LFA 1 : CD11a / CD18 , VLA 4 : CD49d / CD29 and VLA 5 : CD49e / CD29 ) , ICAM 1 ( CD 54 ) and the ' homing receptor ' ( CD 44 ) were investigated by a dual color flow cytometry in 56 cases of B cell disorders namely , 39 chronic lymphocytic leukemias ( CLL ) , four hairy cell leukemia ( HCL ) , seven splenic lymphoma with villous lymphocytes ( SLVL ) and six other non Hodgkin ' s lymphoma ( NHL ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies directed against rat CD 18 and ICAM 1 , as well as TMB 8 , a calcium inhibitor , prevented the calcium mobilization , active oxygen production , and NF kappaB activation in addition to the increased production of NO . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Upregulation of intercellular adhesion molecule 1 ( ICAM 1 ) on follicular epithelium adjacent to lymphocyte function associated antigen 1 ( LFA 1 ) positive folliculotropic lymphoma cells in this report provides insight into lymphocyte homing mechanisms in folliculotropic MF . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Role of the ICAM 1 / LFA 1 pathway during the development of autoimmune dacryoadenitis in an animal model for Sjgren ' s syndrome . ^^^ We detected an up regulation of local cell adhesion molecule genes ( ICAM 1 , LFA 1 , CD 44 and Mel 14 ) in the course of autoimmune lacrimal gland diseases . ^^^ Immunohistochemically , ICAM 1 was localized exclusively in the endothelial cells of variously sized blood vessels before the onset of disease , and LFA 1 , CD 44 and Mel 14 , expressing infiltrating cells , were found within these lesions . ^^^ When the therapeutic effects of blocking cell adhesion molecules in vivo were examined , antibodies to ICAM 1 in combination with anti LFA 1 prevented the development of autoimmune lacrimal gland diseases in NFS / sld mice . ^^^ These data suggest that in Sjgren ' s syndrome like autoimmune dacryoadenitis in NFS / sld mutant mice , the ICAM 1 / LFA 1 pathway may play a crucial role in the development and subsequent progression of T cell mediated autoimmunity in the lacrimal glands . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Bacterial cell wall products increase monocyte HLA DR and ICAM 1 without affecting lymphocyte CD 18 expression . ^^^ The expression of surface class 2 major histocompatibility antigens and the costimulatory receptors CD 18 and CD54 / ICAM 1 ( intercellular adhesion molecule ) was used to evaluate the comparative influence of these immunostimulators . ^^^ On monocytes , both LPS and MDP increased the expression of human leukocyte antigen ( HLA ) DR ( maximal at 6 hr ) , CD 18 ( maximal at 1 3 hr ) , and ICAM 1 ( maximal at 18 24 hr for LPS and 12 hr for MDP ) without increasing the production of superoxide . ^^^ On lymphocytes , only LPS increased ICAM 1 ( after 18 hr ) without affecting CD 18 , and a differential analysis demonstrated a generalized ICAM 1 upregulation in lymphocyte subsets after 18 hr : the most pronounced effect was measured in natural killer cells , followed by CD 8 ( + ) T cells , B cells , and CD 4 ( + ) T cells . ^^^ MDP did not alter ICAM 1 or CD 18 expression on lymphocytes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The effect of p BQ appeared to be specific for IL 2 production , since de novo induction of the IL 2 receptor alpha chain ( CD 25 ) and ICAM 1 ( CD 54 ) and upregulation of LFA 1 alpha / beta ( CD11a and CD 18 ) were unaffected . ^^^ The cigarette tar component p benzoquinone blocks T lymphocyte activation by inhibiting interleukin 2 production , but not CD 25 , ICAM 1 , or LFA 1 expression . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The mAbs reactive with CD11a / CD18 ( leukocyte function associated antigen 1 ; LFA 1 ) and CD 54 ( intercellular adhesion molecule 1 ; ICAM 1 ) completely blocked the IM 9 cell aggregation induced by mNKES , and induction of IM 9 cell aggregation by mNKES was significantly blocked in the presence of the protein kinase C inhibitors sphingosine and H 7 and completely blocked by cytochalasin B and cytochalasin D , which inhibit microfilament formation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Activation of G proteins with AIF 4 induces LFA 1 mediated adhesion of T cell hybridoma cells to ICAM 1 by signal pathways that differ from phorbol ester and manganese induced adhesion . ^^^ We show here that LFA 1 mediated adhesion of TAM2D2 T cell hybridoma cells to ICAM 1 can in fact be induced by direct activation of G proteins using AIF 4 , to the same extent as by using PMA or Mn2+ . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Fluvastatin at 0 . 1 microM or more inhibited the expression of lymphocyte function associated antigen 1 ( LFA 1 ) on U 937 and intercellular adhesion molecule 1 ( ICAM 1 ) on U 937 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Treatment with monoclonal antibodies to ICAM 1 and LFA 1 in rat heart allograft rejection . ^^^ Treatment with monoclonal antibodies to ICAM 1 and LFA 1 ( CD 11 a ) induces specific tolerance after murine heart transplantation . ^^^ Treatment with monoclonal antibodies to ICAM 1 and LFA 1 alone or in combination had no effect on allograft survival after heart transplantation between fully incompatible rat strains . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We investigated the expression of intercellular adhesion molecule 1 ( ICAM 1 ) and lymphocyte function associated antigen 1 ( LFA 1 ) on alveolar macrophages and on lung tissue in the early stage of radiation induced lung injury . ^^^ ICAM 1 and LFA 1 expression on alveolar macrophages was significantly increased starting 1 week after irradiation . ^^^ The expression of ICAM 1 and LFA 1 on lung tissue was not elevated up to 8 weeks after irradiation . ^^^ In conclusion , the increased expression of ICAM 1 and LFA 1 on alveolar macrophages as early as 1 week after irradiation suggests that adhesion molecules play a role in the development of radiation induced lung injury . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The addition of specific MoAbs against ICAM 1 and lymphocyte function associated antigen 1 ( LFA 1 ) produced a high blockage of adhesiveness induced by exposure to PAN ( inhibition 58 + / 3 % ; non stimulated cells 40 + / 7 % ) . ^^^ Simultaneous administration to PAN treated rats of anti LFA 1 and anti ICAM 1 MoAbs reduced the number of interstitial cells by 70 % compared with the 30 % of reduction obtained when anti very late antigen 4 ( VLA 4 ) MoAb and anti vascular cell adhesion molecule 1 ( VCAM 1 ) antibodies were used . ^^^ Our results suggest that the LFA 1 / ICAM 1 pathway plays a principal role in the interstitial nephritis occurring in rats with PAN nephrosis . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Immunohistochemical detection of leucocyte integrins LFA 1 and VLA 4 , their endothelial counter receptors intercellular adhesion molecule 1 ( ICAM 1 ) and vascular cell adhesion molecule 1 ( VCAM 1 ) , and the endothelial cell markers CD 31 and von Willebrand factor related antigen ( vWFAg ) was performed using specific MoAbs and an alkaline phosphatase anti alkaline phosphatase technique . ^^^ These data suggest that leucocyte / endothelial cell interactions mediated by the receptor / ligand pairs LFA 1 / ICAM 1 and VLA 4 / VCAM 1 actively participate in the development of muscle inflammatory infiltrates in the major inflammatory myopathies . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Involvement of leukotrienes , TNF alpha , and the LFA 1 / ICAM 1 interaction in substance P induced granulocyte infiltration . ^^^ Participation of tumor necrosis factor alpha ( TNF alpha ) and LFA 1 / ICAM 1 interaction was confirmed by inhibition of SP induced leukocyte migration by pretreatment of mice with monoclonal antibodies to TNF alpha , LFA 1 , and ICAM 1 . ^^^ These results support the concept that SP modulates in vivo neuroinflammatory responses , as measured by granulocyte migration , initiating a cascade of events that includes LT production , TNF alpha secretion , and engagement of LFA 1 and ICAM 1 . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Circulating leukocytes from MS patients expressed higher levels of LFA 1 , a ligand of intercellular adhesion molecule 1 ( ICAM 1 ) , than did normal leukocytes . ^^^ The data presented here suggest that the ICAM 1 / LFA 1 interaction may determine cytoadherence of leukocytes to brain MVEC in MS . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Flow cytometry analysis of the phenotypic distribution of MNC revealed age related differences in the expression of CD 3 , CD 4 , CD 8 , CD 14 , CD 19 , CD45RA , CD45R0 , CD 2 , LFA 1 , ICAM 1 and LFA 3 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To investigate the effects of monoclonal antibodies ( MAbs ) on IH , we examined the intima / medial ratio of arteries at 2 weeks after injury in five treatment groups : nonimmune IgG , anti membrane glycoprotein MAb , anti lymphocyte function associated antigen 1 ( LFA 1 ) MAb , anti ICAM 1 MAb , and anti ICAM / LFA 1 MAb . ^^^ The intima / media ratios in anti ICAM 1 MAb treated and anti ICAM 1 / LFA 1 MAb treated animals were significantly less than those in nonimmune IgG treated and anti membrane glycoprotein MAb treated animals ( P < . 05 ) . ^^^ MAb against ICAM 1 or ICAM 1 / LFA 1 attenuated IH . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The expression of ICAM 1 or ICAM 2 on RT10 . 3 cells significantly increased the stimulation of T cells in an LFA 1 ( CD11a / CD18 ) dependent manner as determined by thymidine incorporation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
E3 monoclonal antibody directed against the platelet glycoprotein IIb / IIIa cross reacts with the leukocyte integrin Mac 1 and blocks adhesion to fibrinogen and ICAM 1 . ^^^ In this study we explored the binding of 7E3 to monocytic cells and the functional effect of 7E3 and c7E3 Fab on Mac 1 mediated adhesion to fibrinogen ( FGN ) and intercellular adhesion molecule 1 ( ICAM 1 ) , ligands abundant in the injured vessel wall . ^^^ Flow cytometry demonstrated that 7E3 bound to THP 1 monocytic cells and identified a subpopulation ( approximately 10 % ) of Mac 1 that was qualitatively similar to that recognized by CBRM1 / 5 , a mAb directed to an activation specific neoepitope present on a subset of Mac 1 molecules . mAb 7E3 bound to K 562 cells transfected with just the alpha subunit ( CD11b ) of Mac 1 but not to nontransfected cells , confirming a direct interaction between 7E3 and Mac 1 . mAb 7E3 and c7E3 Fab blocked the adhesion of Mac 1 bearing cells to FGN ( 80 + / 11 % and 78 + / 9 % inhibition , respectively ) and ICAM 1 ( 62 + / 14 % and 62 + / 17 % ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Adhesion of monocytes to STs was inhibited > 80 % by antibody to ICAM 1 or its cognate ligand LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
On unstimulated endothelia antibody blockade of LFA 1 or ICAM 1 inhibited T cell line migration to 8 . 0 % and 6 . 8 % of control values , respectively , whereas blocking VLA 4 and VCAM 1 had no effect . ^^^ On IL beta activated endothelium blocking LFA 1 and ICAM 1 was less effective ( 24 . 9 % and 27 . 3 % of control values , respectively ) and blockade of VLA 4 and VCAM 1 brought about a reduction to 63 . 0 % and 68 . 3 % of controls respectively . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Additional studies showed that the cell recruitment mediated by uropods was abrogated with antibodies to ICAM 1 , 3 , and LFA 1 , whereas mAb to CD 43 , CD 44 , CD 45 , and L selectin did not have a significant effect , thus indicating that the interaction of LFA 1 with ICAM 1 and 3 appears to be responsible for this process . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Interleukin 4 induces homotypic aggregation of human mast cells by promoting LFA 1 / ICAM 1 adhesion molecules . ^^^ Flow cytometric analysis of the cultured mast cells showed that IL 4 increases the expression of lymphocyte function associated antigen 1 ( LFA 1 ) and intercellular adhesion molecule 1 ( ICAM 1 ) , but not of very late antigen ( VLA ) family adhesion molecules or vascular cell adhesion molecule 1 ( VCAM 1 ) . ^^^ Neutralizing monoclonal antibodies specific for LFA 1alpha , LFA 1beta , or ICAM 1 inhibited the IL 4 induced homotypic aggregation of the mast cells , indicating that the aggregation was mediated mainly by LFA 1 / ICAM 1 interaction . ^^^ This binding was also inhibited by anti LFA 1 or anti ICAM 1 . ^^^ These results show that IL 4 promotes expression of ICAM 1 and LFA 1 molecules on mast cells , and suggest that IL 4 may contribute to the migration of mast cells into the inflamed tissue and to the cellular interaction with other inflammatory cells by upregulating adhesion molecules . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Lymphocyte function associated antigen 1 ( LFA 1 ) is able to bind selectively to its ligands intercellular adhesion molecules 1 and 3 ( ICAM 1 and ICAM 3 ) , suggesting that LFA 1 can exist in distinct ligand specific binding states . ^^^ In the case of ICAM 1 , apart from ligand itself and the recently cloned molecule cytohesin 1 , the natural physiological regulators of LFA 1 mediated binding to ICAM 1 are unknown . ^^^ We have investigated the role of ligands ( ICAM 1 and ICAM 3 ) in LFA 1 activation by using ICAM blocking monoclonal antibodies and a fixation protocol for `` freezing ' ' LFA 1 on the surface of cells after prior exposure to ICAM 1 and ICAM 3 . ^^^ These studies not only confirm that LFA 1 exists in distinct ICAM specific activation states , but also demonstrate that ICAM 1 plays a role in the activation of LFA 1 binding to ICAM 3 . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
CD11b / CD18 ( Mac 1 ) and CD49d / CD29 ( VLA 4 ) are involved in eosinophil endothelial adhesion through their counterligands , intercellular adhesion molecule 1 ( ICAM 1 ; CD 54 ) and vascular cell adhesion molecule 1 ( VCAM 1 ) , respectively . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In the present investigation , the authors studied the expression and regulation of the beta 2 integrins LFA 1 ( CD11a / CD18 ) , Mac 1 ( CD11b / CD18 ) , p 150 , 95 ( CD11c / CD18 ) and of the LFA 1 / Mac 1 counter receptor intercellular adhesion molecule 1 ( ICAM 1 ; CD 54 ) on leukaemic ( HMC 1 cell subclone 5C6 ) and on normal mature human skin mast cells . ^^^ Increased expression of LFA 1 / ICAM 1 in response to PMA was accompanied by strong enhancement of homotypic cell aggregation , suggesting that newly synthesized LFA 1 / ICAM 1 is functionally active . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
T cell responses to TEC presented Sags were dependent on the presence of the adhesion molecules ICAM 1 , ICAM 2 , LFA 1 , and LFA 3 , but not on CD 4 and CD 8 molecules . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Pretreatment of rats with mAb directed against either CD 18 or ICAM 1 also attenuated the IFN alpha induced microvascular alterations . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We also provide evidence that GCS inhibit cell aggregate formation induced by TCR ligation , which directly correlates with the down modulation of LFA 1 and CD 2 , but not LFA 3 or ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Treatment of target PBMCs with an antibody against LFA 1 , a principal ICAM 1 receptor , was able to nullify the ICAM 1 mediated enhancement . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The intercellular adhesion molecules ICAM 1 , 2 and 3 are , like VCAM 1 , members of the immunoglobulin superfamily ( IgSF ) , but they are recognized by an 1 domain containing integrin , lymphocyte function associated antigen 1 ( LFA 1 , or CD11a / CD18 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Neither LFA 1 nor Mac 1 positive cells were positively correlated with ICAM 1 expression . ^^^ In this study , we could not conclude that LFA 1 / ICAM 1 or Mac 1 / ICAM 1 interaction was involved in the leukocyte accumulation in this disease . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The inflammatory reaction of the glomeruli of the kidneys was studied by immunohistochemical methods using 4 microm cryostat sections stained with specific monoclonal antibodies against neutrophils ( polymorphonuclear cells , PMNs ) and monocytes ( MOs ) , and against the adhesion molecules ICAM 1 and VCAM 1 on the endothelium , and LFA 1 , MAC 1 , and VLA 4 on the leukocytes . ^^^ In C rats there was no increased adhesion molecule expression after endotoxin treatment , in Pr rats there was increased expression of both the combinations ICAM 1 / LFA 1 and VCAM 1 / VLA 4 . ^^^ DEC rats did not express either of these combinations ( although there was expression of ICAM 1 ) ; PSP rats expressed the combination ICAM 1 / MAC 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule 3 ( ICAM 3 ) and possibly ICAM 1 participate as LFA 1 ligands . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In order to better understand the role of lymphocyte function associated antigen 1 ( LFA 1 ) and intercellular adhesion molecule 1 ( ICAM 1 ) molecules in thymocyte maturation , mice were treated from birth with saturating doses of non cytolytic specific monoclonal antibodies . ^^^ Our data demonstrated that the effective saturation of LFA 1 alpha chain in the thymus , but not ICAM 1 or LFA 1 beta chain , selectively interfered with the maturation of CD8+ T cells , as manifested by a marked reduction in the frequency of CD 4 8+ thymocytes expressing high levels of CD 3 and TCR alpha beta . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Temporal analysis of the expression of the adhesion molecules ICAM 1 , LFA 1 , VLA 4 , and VLA 6 was performed . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In HT 29 cells , there was constitutive expression of intercellular adhesion molecule 1 ( ICAM 1 ) and lymphocyte function associated antigen 3 ( LFA 3 ) at a low level , but no constitutive expression of HLA DR , LFA 1 , B 7 1 and B 7 2 molecules . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We have investigated the role of the cytoplasmic domains of LFA 1 in binding to ICAM 1 and in postadhesion events . ^^^ Transfected fibroblasts expressing wild type LFA 1 adhered only weakly to ICAM 1 immobilized on plastic , and phorbol ester pretreatment enhanced this adhesion significantly . ^^^ In contrast , transfected cells expressing LFA 1 lacking both the alpha and the beta cytoplasmic domains , the beta cytoplasmic domain alone , or GPI anchored LFA 1 adhered to immobilized ICAM 1 without prior activation . ^^^ The presence of manganese dramatically enhanced the binding to ICAM 1 of LFA 1 lacking the alpha cytoplasmic domain or both cytoplasmic domains , whereas it had relatively little effect on wild type LFA 1 or the mutant lacking the beta cytoplasmic domain . ^^^ Although doubly truncated or GPI anchored LFA 1 mediated cell adhesion to immobilized ICAM 1 , cells expressing these mutants , as well as those expressing individual alpha and beta chain truncations , failed to spread out following this adhesion , whereas the wild type transfectants did so readily . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Up regulation of ICAM 1 was also observed following stimulation with other mAb specific for CD11a ( S6F1 ) , CD11b ( JML H 11 ) , and CD 18 ( IB 4 ) ; all of these mAb recognized members of the beta 2 integrin family , but not with isotype matched control mAb . ^^^ Intracellular cross talk between LFA 1 and its counter receptor , intercellular adhesion molecule 1 ( ICAM 1 ) , on human monocytic cell line THP 1 was analyzed . ^^^ Stimulation with mAb YH 384 specific for LFA 1 alpha ( CD11a ) up regulated ICAM 1 expression on THP 1 cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In order to study adhesion molecule expression and its consequences for cellular recognition , the presence of adhesion molecules ICAM 1 , VCAM 1 , VLA 4 , LFA 1 , alpha , LFA 1 beta , LFA 3 , beta 1 integrin and beta 3 integrin was studied on specimens from breast tissue by immunohistochemistry and on cells from breast cell lines propagated in vitro . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
They expressed low but positive levels of the CD 14 marker , and intermediate levels of both CD11b ( Mac 1 ) and CD 54 ( ICAM 1 ) adhesion molecules . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The expression of two adhesion molecules , ICAM 1 ( CD 54 ) and ICAM 3 ( CD 50 ) , infiltrating cells positive for their ligand , LFA 1 ( CD11a ) , and the markers of total leukocytes ( CD 45 ) , T cells ( CD 3 ) , granulocytes / monocytes ( CD 15 ) , and macrophages ( CD 68 ) in renal interstitium were examined by an indirect immunoperoxidase method . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule 1 ( ICAM 1 , CD 54 ) is a member of the Ig superfamily and is a counterreceptor for the beta 2 integrins : lymphocyte function associated antigen 1 ( LFA 1 , CD11a / CD18 ) , complement receptor 1 ( MAC 1 , CD11b / CD18 ) , and p 150 , 95 ( CD11c / CD18 ) . ^^^ In this report , we describe a cell free assay using purified recombinant extracellular domains of LFA 1 and a dimeric immunoadhesin of ICAM 1 . ^^^ The binding of recombinant secreted LFA 1 to ICAM 1 is divalent cation dependent ( Mg2+ and Mn2+ promote binding ) and sensitive to inhibition by antibodies that block LFA 1 mediated cell adhesion , indicating that its conformation mimics that of LFA 1 on activated lymphocytes . ^^^ Thirty five point mutants of the ICAM 1 immunoadhesin were generated and residues important for binding of monoclonal antibodies and purified LFA 1 were identified . ^^^ These mutants , along with modeling studies , define the LFA 1 binding site on ICAM 1 as residues E 34 , K 39 , M 64 , Y 66 , N 68 , and Q 73 , that are predicted to lie on the CDFG beta sheet of the Ig fold . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Intracellular signals are required to activate the leukocyte specific adhesion receptor lymphocyte function associated molecule 1 ( LFA 1 ; CD11a / CD18 ) to bind its ligand , intracellular adhesion molecule 1 ( ICAM 1 ) . ^^^ In contrast to interleukin 2 / phytohemagglutinin activated peripheral blood lymphocytes , resting lymphocytes , which display a homogenous cell surface distribution of LFA 1 , respond poorly to intracellular signals to bind ICAM 1 , unless the actin cytoskeleton is disrupted . ^^^ On resting peripheral blood lymphocytes , uncoupling of LFA 1 from the actin cytoskeleton induces clustering of LFA 1 and this , along with induction of a high affinity form of LFA 1 , via `` inside out ' ' signaling , results in enhanced binding to ICAM 1 , which is dependent on intact intermediate filaments , microtubules , and metabolic energy . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
When we compared the FP group with the placebo group , we observed a significant decrease only in the number of cells expressing intracellular adhesion molecule 1 ( ICAM 1 ) and MAC 1 ( p < 0 . 04 and p < 0 . 03 , respectively ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We assessed the expression pattern of intercellular adhesion molecule 1 ( ICAM 1 ) and lymphocytefunction associated antigen 1 ( LFA 1 ) in the livers of mice experimentally infected with Schistosoma mansoni and in synchronous hepatic granulomas induced by injection of soluble egg antigen ( SEA ) coupled Sepharose beads in a mesenteric vein of mice . ^^^ ICAM 1 and LFA 1 immunoreactive cells were present in the granuloma as early as at 3 days after injection of SEA coupled beads and persisted with time . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
DLC stimulated MLR was inhibited by monoclonal antibodies ( mAbs ) to B 7 1 , B 7 2 , intracellular adhesion molecule 1 ( ICAM 1 ) , vascular cell adhesion molecule 1 ( VCAM 1 ) , leucocyte function associated antigen 1 ( LFA 1 ) or very late activation antigen 4 ( VLA 4 ) by 30 55 % . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
These data suggest that decreased expression of adhesion molecules including VLA 4 , LFA 1 , ICAM 1 and LFA 3 play a role in PC mobilization . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The decreased adhesion rate after treatment with NA 382 for 48 hr was not further inhibited by addition of monoclonal antibodies of leukocyte function associated antigen 1 ( LFA 1 ) alpha and beta chains and intercellular adhesion molecule 1 ( ICAM 1 ) ( WT . 1 , WT . 3 , and 1A29 , respectively ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
RESULTS : PCFCCLs of the head and trunk expressed ICAM 1 ( 80 % ) and LFA 1 ( 50 % ) much more frequently than PCFCCLs of the legs ( 40 % and 20 % , respectively ) and SCFCCLs ( 14 % and 14 % , respectively ) . ^^^ Absence of both ICAM 1 and LFA 1 on the neoplastic B cells correlated with a poor prognosis ( seven of nine patients died of systemic lymphoma ) . ^^^ In contrast , none of the patients with expression of LFA 1 or ICAM 1 have died of lymphoma thus far . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The present study was undertaken to determine whether polymorphonuclear neutrophils ( PMNs ) can contribute to hepatic ischemia reperfusion injury , and pretreatment with monoclonal antibodies ( mAbs ) to intercellular adhesion molecule 1 ( ICAM 1 ) , lymphocyte function associated antigen 1 ( LFA 1 ) , and CD 18 could improve energy metabolism and prolong the viability of the organ . ^^^ Monoclonal antibodies to ICAM 1 , LFA 1 , or CD 18 were injected intravenously 5 minutes before inducing ischemia . ^^^ RESULTS : Although ischemia of the liver for 90 minutes did not permit survival of the animals , pretreatment with mAbs to ICAM 1 plus LFA 1 increased the survival rate to 57 % . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The expression of intercellular adhesion molecule 1 ( CD 54 or ICAM 1 ) on epithelial cells during acute or chronic inflammation may favor the interaction between epithelial cells and leukocytes expressing the natural ligands of ICAM 1 , LFA 1 ( CD11a / CD18 ) , and Mac 1 ( CD11b / CD18 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
These same LGL cell lines also expressed high levels of lymphocyte function associated antigen 1 ( LFA 1 ) and intracellular adhesion molecule 1 ( ICAM 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We found that the integrin adhesion molecules leukocyte function antigen 1 ( LFA 1 ) , very late antigen 4 ( VLA 4 ) , and the ligand for LFA 1 , intercellular adhesion molecule 1 ( ICAM 1 ) , were expressed on microglial cells accumulating in the outer molecular layer of the deafferented dentate gyrus . ^^^ Double fluorescence labeling demonstrated that microglial cells engaged in the phagocytosis of axonal debris expressed LFA 1 , VLA 4 , and the LFA 1 ligand ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Cyclic peptide 1 inhibits homotypic adhesion of T cells ( Molt 3 ) mediated by ICAM 1 and the leukocyte function associated antigen 1 ( LFA 1 ) on the surface of T cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Lack of in vitro synergy of clinically used antibodies specific for ICAM 1 and LFA 1 . ^^^ In a murine model a combined though not a single application of antibodies against LFA 1 and ICAM 1 can induce tolerance towards heart transplants . ^^^ To fill this blank we tested the immunosuppressive capacity of ANTILFA ( alpha LFA 1 ) and BIRR 1 ( alpha ICAM 1 ) on human cells in vitro alone and in combination . ^^^ Most assays showed a similar immunosuppressive effect of LFA 1 and ICAM 1 blocking while the combination of both antibodies was not significantly more effective than each antibody alone . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To study the adhesive properties of the LFA 1 1 domain , we stably expressed a GPI anchored form of this 1 domain ( 1 GPI ) on the surface of baby hamster kidney cells . 1 GPI cells bound soluble ICAM 1 ( sICAM 1 ) with a low avidity and affinity . ^^^ The LFA 1 activating antibody MEM 83 , or its Fab fragment , decreased the rolling velocity of 1 GPI cells on ICAM 1 containing membranes . ^^^ This suggests that the 1 domain acts as a transient , Mg2+ dependent binding module that cooperates with another Mn2+ stimulated site in LFA 1 to give rise to the stable interaction of intact LFA 1 with ICAM 1 . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Expression of adhesion molecules ( leucocyte functional associated antigen 1 ( LFA 1 ) , intercellular adhesion molecule 1 ( ICAM 1 ) ) and CD 30 were evaluated in patient subgroups . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibody ( mAb ) R6 . 5 against ICAM 1 and mAb CLB LFA1 / 1 against CD 18 both reduced the migration of PMNs across mesothelial monolayers with a predominant inhibitory effect of CLB LFA1 / 1 , indicating a significant role of the beta ( 2 ) integrins of PMNs in this process . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The blocking effects of anti intercellular adhesion molecule 1 ( ICAM 1 ) and anti lymphocyte function associated antigen 1 ( LFA 1 ) monoclonal antibodies ( Mabs ) were also studied . ^^^ Anti ICAM 1 and anti LFA 1 Mabs inhibited LAK mediated cytotoxicity . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The intensity and extent of HLA DR and ICAM 1 LFA 1 staining were closely correlated with the degree of tubulointerstitial damage of HFRS . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
CD 99 ( MIC 2 ) regulates the LFA 1 / ICAM 1 mediated adhesion of lymphocytes , and its gene encodes both positive and negative regulators of cellular adhesion . ^^^ Here we report that the CD 99 gene encodes two distinct proteins with opposite functions in the LFA 1 / intercellular adhesion molecule 1 ( ICAM 1 ) mediated cell adhesion process . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Using flow cytometry , high concentrations of ICAM 1 , VCAM 1 , and NCAM antigens were detected on undifferentiated ES cells , but their specific receptors , Mac 1 , LFA 1 , and VLA 4 , were not detected . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
ICAM 1 / LFA 1 expression in acute osteodestructive joint lesions in collagen induced arthritis in rats . ^^^ Indirect immunohistochemistry was performed on undecalcified cryostat sections for intercellular adhesion molecule 1 ( ICAM 1 , clone 1A 29 ) and leukocyte function associated antigen 1 ( LFA 1 , clone WT . 1 ) expression , for CD4+ lymphocytes ( clone W3 / 25 ) , B cells ( clone HIS 14 ) , and macrophages ( clone ED 2 ) . ^^^ The results suggest that LFA 1 and ICAM 1 mediate important inflammatory events in this model . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Because adhesion molecules are proposed to play a crucial role in T cell development , we investigated CD 44 , LFA 1 , and ICAM 1 expression in such complexes . ^^^ With regard to ROS , all subsets expressed CD 44 , and macrophage and dendritic cell ROS were also ICAM 1 positive and LFA 1 positive . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The LAK and ADCC activities could be effectively distinguished based on our observation that monoclonal anti rat ICAM 1 and anti rat LFA 1 antibodies inhibited only LAK activity , but not ADCC activity , in addition , anti AK 5 antiserum , but not purified anti AK 5 antibody , inhibited LAK activity , suggesting that soluble ICAM 1 present in hyperimmune serum could be responsible for inhibition of LAK activity . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule 1 ( ICAM 1 ) is a major ligand for 2 members of the CD 18 family of leukocyte integrin adhesion molecules and mediates adhesion between leukocytes and stimulated endothelial cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Lymphocyte function associated antigen 1 ( LFA 1 ) on the surface of activated T cells did not bind soluble ICAM 1 dimers with high affinity . ^^^ This threshold was eliminated , and all forms of ICAM 1 bound to LFA 1 when LFA 1 was converted to a high affinity form with an activating antibody . ^^^ We show that high affinity LFA 1 is generated only as a consequence of an initial low affinity interaction of LFA 1 with ICAM 1 under physiological conditions . ^^^ Therefore , the selectivity of cell surface LFA 1 for cell sized particles bearing ICAM 1 appears to be due to the maintenance of low affinity LFA 1 on the surface of activated T cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In addition to these immunosuppressive agents , monoclonal antibodies against functional cell surface molecules , such as LFA 1 , ICAM 1 , Fas , and Fas ligand , must be effective for treatment of TA GVHD . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Leukocyte and endothelial cell adhesion molecules ( CAMs ) are essential for emigration of leukocytes , with the selectins mediating the initial step of leukocyte `` rolling ' ' and the beta 2 ( CD 18 ) integrins and intercellular adhesion molecule 1 ( ICAM 1 ) being important for firm adhesion and emigration . ^^^ Using C57BL / 6 mice fed a high fat diet , we observed a 50 % to 75 % reduction in atherosclerotic fatty streaks in mice with homozygous mutations for ICAM 1 , P selectin , CD 18 , both ICAM 1 and CD 18 , or both ICAM 1 and P selectin . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The stable adhesion of these cell types to endothelial cells is due to the molecular interaction of the integrins VLA 4 and LFA 1 with their ligands vascular cell adhesion molecule ( VCAM ) and ICAM 1 present on the endothelial cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
RESULTS : In patients , we found an increased expression of several adhesion molecules ICAM 1 , LFA 3 and MAC 1 in lymphocytes , LFA 3 in monocytes and surface activation markers CD 71 and DR in lymphocytes , as well as increased concentrations of the serum parameters measured : IgA , IgG , IgM , interleukin 1 beta , interleukin 6 , tumor necrosis factor alpha , soluble ICAM 1 and neopterin , in comparison with controls . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The effect of antibodies against cell surface adhesion molecules ( LFA 1 alpha and ICAM 1 ) on the migration and localization of 99mTc labeled leukocytes in acute infection . ^^^ We investigated the effects of blocking anti LFA 1 alpha and ICAM 1 antibody treated 99mTc labeled leukocytes on the migration and localization to the site of E . coli induced acute infection in CBA / J mice . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Anti LFA 3 Abs , in the absence or presence of IL 12 , inhibit EC costimulation of IFN gamma production by up to 50 % , while Abs to intercellular adhesion molecule 1 ( ICAM 1 ) , vascular cell adhesion molecule 1 ( VCAM 1 ) , LFA 1 , and very late antigen 4 ( VLA 4 ) have relatively little effect . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Notably , antibodies directed against VLA 4 , other beta 2 integrins ( Mac 1 and p 150 ) , or other potential LFA 1 counterstructures that were expressed on the cell surface ( ICAM 1 and ICAM 3 ) did not block aggregation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The panel included probes specific for the CD 45 , CD 34 , P glycoprotein ( mdr 1 ) , Ly 6A / E ( Sca 1 ) , heat stable antigen ( CD 24 ) , Fc receptor for IgG FcgammaRII ( CD 32 ) , CD 44 , CD 22 , and ICAM 1 ( CD 54 ) genes , as well as alphaL ( CD11a ) , alphaM ( CD11b ) , beta 2 ( CD 18 ) , alpha 4 ( CD49d ) , alpha 5 ( CD49e ) , beta 1 ( CD 29 ) , and beta 7 integrin subunit sequences . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We investigated the effects of monoclonal antibodies ( mAbs ) against lymphocyte function associated antigen 1 ( LFA 1 ) and intercellular adhesion molecule 1 ( ICAM 1 ) on intrasplenic allogenic and xenogenic hepatocyte transplantation ( HCTx ) to analbuminemic rats . ^^^ The experimental groups were as follows : group 1 , allo HCTx ( n = 7 ) ; group 2 , allo HCTx + antirat ICAM 1 / antirat LFA 1 mAbs ( 1 . 0 mg / kg / day , for 7 days , respectively ) ( n = 6 ) ; group 3 , xeno HCTx ( n = 5 ) ; group 4 , xeno HCTx + mAbs ( antimouse LFA 1 / antirat ICAM 1 ) ( n = 5 ) . group 5 , xeno HCTx + mAbs ( antirat LFA 1 / antimouse ICAM 1 ) ( n = 5 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Stable adhesion and transmural migration predominantly involve integrins ( LFA 1 , etc . ) and members of the immunoglobulin supergene family ( ICAM 1 , etc ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
VCAM 1 and ICAM 1 were significantly ( P < 0 . 05 ) increased after 4 d of G CSF administration as was neutrophil cell surface expression of CD11b and CD 18 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Regulation by differential development of Th 1 and Th 2 cells in peripheral tolerance to cardiac allograft induced by blocking ICAM 1 / LFA 1 adhesion . ^^^ BACKGROUND : Specific immune tolerance to cardiac allografts is induced by anti ICAM 1 and anti LFA 1 MAbs . ^^^ Although the expression of the Th 1 cytokines IL 2 and IFN gamma is shown to increase in association with acute rejection , the roles of cytokines in the induction of peripheral tolerance by anti ICAM 1 and anti LFA 1 MAbs are not yet known . ^^^ The MAbs to ICAM 1 and LFA 1 were injected for 3 days after transplantation in some recipients , and others were treated with FK 506 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In this paper , we examined the effects of monocyte isolation , adherence during culture and endotoxin exposure on the expression of these molecules and the ligand for LFA 1 , ICAM 1 ( CD 54 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
This investigation is concerned with the nurse cell expression of the intercellular adhesion molecule ICAM 1 , the ligand for thymocyte LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Mean transcript levels of the adhesion molecules P selectin , intercellular adhesion molecule 1 ( ICAM 1 ) , and leukocyte function associated antigen 1 ( LFA 1 ) and the cytokines interleukin 4 ( IL 4 ) , interferon gamma ( IFN gamma ) , inducible nitric oxide synthase ( iNOS ) , allograft inflammatory factor 1 ( AIF 1 ) , and monocyte chemoattractant protein 1 ( MCP 1 ) were measured with reverse transcription polymerase chain reaction [ RT PCR ] assays using deoxycytidine triphosphate radiolabeled with phosphorus 32 [ 32P dCTP ] . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Since leukocyte function associated antigen 1 ( LFA 1 ) binds leukocytes to cells through ICAM 1 , initiating inflammation , Zn2+ is expected to block LFA 1 / ICAM 1 binding and thereby suppress inflammation . ^^^ Zinc ions may be an important anti inflammatory factor because they can block docking of both HRV and LFA 1 with ICAM 1 . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
It was induced by TNF and interferon gamma and depended on LFA 1 expressed on the platelet surface and ICAM 1 expressed on MVECs . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The number of backcrosses ranged between 8 ( P selectin ) and 10 ( CD 18 and ICAM 1 ) generations . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
This work investigates the expression of three different cell surface markers [ macrophages MAC 1 , MAC 3 and intercellular adhesion molecule 1 ( ICAM 1 ) ] on macrophages in vitro using confocal microscopy and shows that ICAM 1 is also a marker of macrophage activation in this model . ^^^ We observed increased amounts of ICAM 1 on activated macrophages compared to unactivated macrophages , whereas MAC 1 and MAC 3 were either expressed constitutively or demonstrated no quantitative change in expression after activation under the same experimental conditions . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NF kappa B activation correlated with the ability of CD 40 to induce Ab secretion and the up regulation of ICAM 1 and LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The basis for a cellular immune response is formed by the interaction between T lymphocytes and antigen presenting cells amplified by adhesion molecule LFA 1 , 2 , 3 to ICAM 1 binding . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Kinetics of the expression of LFA 1 alpha / CD11a , LFA 1 beta / CD18 , Mac 1 / CD11b , VLA 4 / CD49d , ICAM 1 / CD54 and L selectin / CD62L was determined on splenic CD4+ and CD8+ T cells and macrophages by flow cytometry . ^^^ In the spleen , a sustained increase in LFA 1 expression and a temporary increase at day 7 in the expression of VLA 4 , Mac 1 and ICAM 1 were detected on CD8+ T cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Role of CD 18 ICAM 1 in the entrapment of stimulated leukocytes in alveolar capillaries of perfused rat lungs . ^^^ Pretreatment with monoclonal antibodies against intercellular adhesion molecule 1 ( ICAM 1 ) or CD 18 attenuated these changes . ^^^ These results suggest that selectin independent , CD 18 ICAM 1 dependent capillary sequestration is one of the major mechanisms by which activated leukocytes accumulate in the lungs . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Adhesion molecule LFA 1 / ICAM 1 influences on LPS induced megakaryocytic emperipolesis in the rat bone marrow . ^^^ To investigate the effect of adhesion molecules on the occurrence of megakaryocytic emperipolesis of neutrophils , we examined the expression of lymphocyte function associated antigen 1 ( LFA 1 ) and intercellular adhesion molecule 1 ( ICAM 1 ) in the bone marrow of lipopolysaccharide ( LPS ) treated rats ( experiment 1 ) and the occurrence of megakaryocytic emperipolesis in anti LFA 1 antibody treated rats ( experiment 2 ) . ^^^ These findings suggest that the occurrence of megakaryocytic emperipolesis is partly dependent on adhesion molecules via LFA 1 / ICAM 1 . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The absence of Mac 1 interactions with obvious ligands , intercellular adhesion molecule 1 ( ICAM 1 ) , and C 3 complement , is not responsible for the decrease in neutrophil accumulation in Mac 1 deficient mice since glomerular PMN accumulation in mice deficient in these ligands was comparable to those in wild type mice . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Inhibition of mononuclear cell recruitment in aortic intima by treatment with anti ICAM 1 and anti LFA 1 monoclonal antibodies in hypercholesterolemic rats : implications of the ICAM 1 and LFA 1 pathway in atherogenesis . ^^^ To investigate the mechanism ( s ) for mononuclear cell recruitment in the arterial wall during the development of atherosclerosis , we studied intercellular adhesion molecule 1 ( ICAM 1 ) and lymphocyte function associated antigen 1 ( LFA 1 ) expression in aortic intima from diet induced hypercholesterolemic rats . ^^^ To examine the participation of ICAM 1 and LFA 1 in adherence and migration of mononuclear cells , we administered monoclonal antibodies ( mAb ) against either ICAM 1 or LFA 1 into hypercholesterolemic rats after they were fed a cholesterol rich diet for 2 weeks . ^^^ Two weeks after the mAb treatment , the number of macrophages adhering to the intima was significantly inhibited by 42 % ( p < 0 . 001 ) with anti ICAM 1 mAb and by 31 % ( p < 0 . 001 ) with anti LFA 1 mAb compared to controls injected with mouse IgG . ^^^ These results suggest that the ICAM 1 and LFA 1 pathway is involved in mononuclear endothelial cell interaction during cholesterol rich diet induced atherogenesis . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
These data suggest that recruitment of CD 4 cells to the GT is mediated through LFA 1 : ICAM 1 and alpha4beta7 : MAdCAM 1 VCAM 1 interactions . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
METHODS : We have assessed interstitial and glomerular leucocytes by avidin biotin peroxidase with monoclonal antibodies ( MA ) against leucocytes ( CD 45 ) , beta 2 integrin ( CD 18 ) , monocyte macrophages ( CD 14 ) , T ( CD 3 ) and T cell subsets ( CD 4 , CD 8 ) , and intercellular adhesion molecule 1 ( ICAM 1 ) ( CD 54 ) , and analysed their relation with the abnormal expression of ICAM 1 on proximal tubule epithelium in sequential renal sections from 48 patients with IgAN stratified according to the severity of the interstitial cellular infiltration observed by light microscopy . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
LFA 1 , the main ICAM 1 counter receptor , was found to be highly expressed on lymphocytes and monocytes , especially at the vicinity of damaged fibers . ^^^ These findings suggest that in IPM and HIV PM , enhanced ICAM 1 and LFA 1 expression possibly induced by TNF alpha , may regulate the homing process of selected lymphocyte clones in muscle tissue . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
RESULTS : Lymphoid cells in CVS derived decidua expressed CD 56 , PECAM , intracellular adhesion molecule 1 ( ICAM 1 ) , and the integrins LFA 1 , alpha E beta 7 , and alpha 4 beta 1 , and co receptors for these molecules were expressed by decidual stromal cells , invasive trophoblasts , and venule endothelium . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The degenerating nerves were invaded by Mac 1 , LFA 1 , and F4 / 80 positive macrophages ; significantly lower numbers of macrophages were present in ICAM 1 deficient nerves . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We found that antiintercellular adhesion molecule 1 ( ICAM 1 ) and antilymphocyte function associated antigen 1 ( LFA 1 ) monoclonal antibodies ( mAbs ) induce immunologic tolerance to mice with cardiac allografts . ^^^ METHODS : To evaluate the effects of short term administration of anti ICAM 1 plus anti LFA 1 mAbs in preventing graft arteriopathy , we treated C3H / He mice that received cardiac allografts from BALB / c mice with anti ICAM 1 plus anti LFA 1 mAbs for the first 5 days after transplantation . ^^^ RESULTS : Allografts from mice that received FK 506 treatment daily showed significant neointimal thickening with increased expression of ICAM 1 , VCAM 1 , and PDGF B mRNA , whereas there was almost no intimal thickening and ICAM 1 , VCAM 1 , and PDGF B mRNA expression in the mice that received anti ICAM 1 plus anti LFA 1 mAbs . ^^^ CONCLUSION : Short term blockade of ICAM 1 and LFA 1 adhesion not only induces immunologic tolerance to cardiac allografts but also prevents graft arteriopathy . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Assessment of tolerance induction to cardiac allograft by anti ICAM 1 and anti LFA 1 monoclonal antibodies . ^^^ BACKGROUND : Transplantation tolerance is induced by selective manipulation of intercellular adhesion molecule 1 ( ICAM 1 ) and leukocyte function associated antigen 1 ( LFA 1 ) molecules in the adult mouse . ^^^ METHODS AND RESULTS : C3H / He mice were heterotopically transplanted with BALB / c hearts ; recipients were injected with anti ICAM 1 and anti LFA 1 monoclonal antibodies ( mAbs ) . ^^^ CONCLUSIONS : These results demonstrate the unique character of the peripheral tolerance induced by anti ICAM 1 and anti LFA 1 mAbs . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Increased expression of neutrophil and monocyte adhesion molecules LFA 1 and Mac 1 and their ligand ICAM 1 and VLA 4 throughout the acute phase of myocardial infarction : possible implications for leukocyte aggregation and microvascular plugging . ^^^ METHODS : We measured the expression of neutrophil and monocyte lymphocyte function associated antigen 1 ( LFA 1 ) , Mac 1 , very late after activation antigen 4 ( VLA 4 ) and intercellular adhesion molecule 1 ( ICAM 1 ) by flow cytometry throughout the acute phase of acute myocardial infarction in 25 patients and 10 age matched control subjects . ^^^ The expression of LFA 1 , Mac 1 , VLA 4 and ICAM 1 on the monocyte cell membrane in patients with an acute myocardial infarction was increased compared with that in control subjects by 22 % ( on day 7 ) , 67 % , 13 % and 44 % ( all on day 4 ) , respectively ( all p < 0 . 001 ) . ^^^ In addition , the manifestation of increased density of LFA 1 and Mac 1 by activated leukocytes with monocytes also expressing ICAM 1 suggests that leukocytes may form microaggregates that could cause microvascular plugging . ^^^ RESULTS : Expression of Mac 1 on neutrophils increased significantly , whereas no expression of VLA 4 and ICAM 1 was detected . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Significantly higher expression of L selectin and CD 44 , and significantly lower expression of VLA 4 , LFA 1 , ICAM 1 , Sialyl Lewis ( 10 ) , Sialyl Lewis ( A ) , and Thy 1 were observed on PBSCs compared with BM CD34+ cells . ^^^ The log of the number of reinfused CD34+ cells , colony forming units granulocyte / macrophage ( CFU GM ) , and CD34+ cells coexpressing VLA 4 , VLA 5 , LAF 1 , Mac 1 , LFA 3 , or CD 38 but not ICAM 1 , Sialyl Lewis ( 10 ) , Sialyl Lewis ( A ) , or Thy 1 correlated with the time required to reach an absolute neutrophil count ( ANC ) of > or =0 . 5 10 10 ( 9 ) / L . ^^^ The log of the number of reinfused CD34+ cells , CFU GM , and CD34+ cells coexpressing CD 44 , L selectin , VLA 5 Mac 1 , or CD 38 , but not VLA 4 , LAF 1 , ICAM 1 , LAF 3 , Sialyl Lewis ( 10 ) , Sialyl Lewis ( A ) , or Thy 1 , correlated with the time required to reach a platelet count of > 20 10 10 ( 9 ) / L . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The absence of changes in the expression of vascular cell adhesion molecule , integrin ligands ( CD11a , CD 18 , integrin alpha 4 ) , and sialyl Lewis 10 indicates a specific effect of soluble E selectin on ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Among a large panel of antibodies tested only mAbs against CD 19 induced homotypic adhesion of B CLL cells via the adhesion molecules integrin alphaL ( leukocyte function antigen 1 ( LFA 1 ) ) , intercellular adhesion molecule 1 ( ICAM 1 ) and CD 21 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule 1 ( ICAM 1 ) is an inducible glycoprotein that interacts with the leukocyte beta 2 integrins , LFA 1 and Mac 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Antibodies to other adhesion receptors such as the integrin beta 2 subunit , ICAM 1 or CD 44 have no effect on human cell binding to these stromas . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
METHODS : With flow cytometric analysis we have investigated the expression of the costimulatory molecules B 7 1 ( CD 80 ) , B 7 2 ( CD 86 ) , and CD 18 and the intercellular adhesion molecule 1 ( ICAM 1 ) on peripheral blood monocytes and the expression of the activation markers HLA DR and IL 2R ( CD 25 ) on peripheral blood T lymphocytes from 31 CD patients , 17 ulcerative colitis ( UC ) patients , and 10 healthy controls . ^^^ Most monocytes from all three groups expressed B 7 2 , CD 18 , and ICAM 1 molecules ( all > 79 % ) , but only a few positive cells expressed B 7 1 molecules ( < 5 % ) . ^^^ However , in CD patients there was a significantly increased MFI of B 7 2 , CD 18 , and ICAM 1 molecules compared with UC and controls ( P < 0 . 05 ) . ^^^ CONCLUSIONS : Expression of the costimulatory molecules B 7 2 , CD 18 , and ICAM 1 on peripheral blood monocytes of CD patients is increased . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
All patients had a pre ECP biopsy with a grade 2 or 3 rejection ( Banff ) with a diffuse infiltrate CD 8 , CD 14 , LFA 1 ( 166 cells positive / 0 . 048 mm 2 ) , and VLA 4 ( 51 cells positive / 0 . 048 mm 2 ) positive , as well as a tubular expression of HLA DR ( 6 . 2 sections of tubule positive / 0 . 048 mm 2 ) , ICAM 1 , and VCAM 1 ( 3 . 1 and 2 . 9 sections of tubule positive / 0 . 048 mm 2 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Blocking studies with anti LFA 1 and anti ICAM 1 monoclonal antibodies caused an inhibition of the attachment to baseline levels , demonstrating that the enhancement of S 20 attachment was dependent upon the LFA 1 / ICAM 1 interaction . ^^^ These results suggest that LFA 1 expressing leukocytes may act as a bridge between the endothelium and tumor cells which express ICAM 1 and , thereby , enhance the potential for hematogenous metastasis . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The 1 domain of integrin LFA 1 interacts with ICAM 1 domain 1 at residue Glu 34 but not Gln 73 . ^^^ Using a solid phase assay , we show that isolated LFA 1 1 domain binds ICAM 1 in a Mg2+ dependent manner and is blocked by anti 1 domain monoclonal antibodies . ^^^ In ICAM 1 , domain 1 residues Glu 34 and Gln 73 have been identified as critical for binding of LFA 1 as an intact receptor ( Staunton , D . ^^^ Thus , we describe the interaction between the LFA 1 1 domain and ICAM 1 , an event that controls the function of the intact receptor but includes only part of the complete ligand binding site . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The aim of the present investigation was to study the distribution of CD11b , CD 18 , and ICAM 1 over the various cell populations present in the margin zone of the actively spreading psoriatic lesion and distant uninvolved psoriatic skin . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Dithiothreitol ( DTT ) and other dithiol antioxidants with closely spaced thiol pairs strongly activate leukocyte function antigen 1 ( LFA 1 , alphaLbeta 2 integrin ) to bind intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^ Phenylarsine oxide ( PAO ) , an oxidant selectively reactive with closely spaced pairs of thiol groups , inhibited LFA 1 dependent adhesion of human natural killer and HSB 2 T leukemia cells to murine cells expressing human ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The interactions of intercellular adhesion molecules 1 and 3 ( ICAM 1 and ICAM 3 ) with lymphocyte function associated antigen 1 ( LFA 1 ) have been characterized and compared on the molecular and cellular level . ^^^ Enzyme linked immunosorbent based molecular assays have been utilized to calculate the binding affinities of soluble ICAM 1 ( sICAM 1 ) and soluble ICAM 3 ( sICAM 3 ) for LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In order to understand the mechanism of unresponsiveness towards Mycobacterium leprae antigens in leprosy , we evaluated the role of M . leprae sonicate antigens in regulating the expression of the costimulatory molecules B 7 1 , CD 28 , intercellular adhesion molecule 1 ( ICAM 1 ) , LFA 1alpha , LFA 1beta and Mac 1 on the lymphocytes of both leprosy patients and healthy subjects . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Both BCP 1 and HBL 6 cells lack expression of important cytoadhesion molecules including CD11a and CD 18 ( LFA 1 alpha and beta chains ) , CD 29 , CD 31 , CD 44 , CD 54 ( ICAM 1 ) , and CD62L and E ( L and E selectins ) . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Immunohistochemistry was performed for CD 18 , CD11a , CD11b , CD11c , alphad , ICAM 1 , ICAM 2 , and ICAM 3 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Immunohistochemical study showed hyper expression of ICAM 1 on vascular endothelial cells and expression of LFA 1 on mononuclear infiltrating cells in the spinal cords of TMEV infected mice . ^^^ Treatment with mAb to ICAM 1 and / or LFA 1 molecules resulted in significant suppression of the development of demyelinating disease , both clinically and histologically , with down regulation in the CNS of the respective adhesion molecules after treatment . ^^^ These data suggest that ICAM 1 / LFA 1 interaction is critically involved in the pathogenesis of TMEV IDD and that antibodies to these adhesion molecules could be a novel therapeutic approach to the treatment of demyelinating diseases such as human multiple sclerosis . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Kupffer cells constitutively expressed LFA 1 and smaller amounts of MAC 1 , and high levels of ICAM 1 were constitutively expressed by liver sinusoidal lining cells , portal tract , and central vein endothelia . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Recent studies from our laboratory and by others indicate that administration of antibodies directed against P selectin , ICAM 1 , or the common subunit ( CD 18 ) of CD11 / CD18 was as effective as neutrophil depletion in attenuating ischemia / reperfusion ( I / R ) induced microvascular barrier disruption and edema formation in skeletal muscle . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Adhesion molecules such as ICAM 1 , Mac 1 , and LFA 1 may help regulate interactions of immune cells and inflammatory cells . ^^^ The increased expression of ICAM 1 is linked with massive infiltration of inflammatory cells that express LFA 1 and Mac 1 , and also with APCs that express HLA DR , suggesting that ICAM 1 exerts a key role in immuno inflammatory responses in gastric mucosa of patients with H . pylori associated gastritis . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To elucidate this process , the present work analyzes the kinetics of the expression of intercellular adhesion molecule 1 ( ICAM 1 ) and the accumulation of inflammatory leukocyte function antigen 1 ( LFA 1 ) positive cells in relation to the appearance of hepatocellular necrosis in the model of acute carbontetrachloride ( CCl 4 ) induced liver injury . ^^^ ICAM 1 and LFA 1 immunoreactivity was analyzed in normal livers and in livers obtained 3 , 6 , 9 , 12 , 18 , 24 , 48 , and 72 hours after CCl 4 administration , as well as in liver cells isolated 3 , 6 , 9 , 12 , 18 , and 24 hours after CCl 4 administration . ^^^ These data demonstrate an early up regulation of ICAM 1 in liver cells and the accumulation of LFA 1 expressing cells prior to the development of necrotic areas . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Therefore , we investigated whether alimemazine applied at pharmacologic doses would modify the expression of thyroid antigens , and simultaneously , the expression of intercellular adhesion molecule 1 ( ICAM 1 ) , B 7 , and LFA 1 co receptors in human TEC in culture . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Peptides or small molecules that can block the interaction of the integrin Mac 1 with its receptor , intercellular adhesion molecule 1 ( ICAM 1 ) , have not previously been developed . ^^^ We studied this interaction by measuring the adherence of ICAM 1 expressing Chinese hamster ovary ( CHO ) cells to immobilized , purified Mac 1 . ^^^ We have recombinantly produced Mac 1 1 domain , and show that it is also capable of supporting the adherence of ICAM 1 expressing CHO cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We used a panel of specific monoclonal antibodies ( mAbs ) directed against intercellular adhesion molecule 1 ( ICAM 1 ) , intercellular adhesion molecule 3 ( ICAM 3 ) , lymphocyte function associated antigen 1 ( LFA 1 ) , very late activation antigen 4 ( VLA 4 ) , vascular cell adhesion molecule 1 ( VCAM 1 ) , and endothelial leucocyte adhesion molecule 1 ( ELAM 1 ) In addition , a panel of mAbs were used to characterise the composition of the inflammatory infiltrate . ^^^ RESULTS : In the normal conjunctiva , ICAM 1 was expressed on the vascular endothelium only , LFA 1 and ICAM 3 on epithelial and stromal mononuclear cells , and VLA 4 on stromal mononuclear cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The objective of the present study is to apply a novel side view imaging technique to investigate T leukemic Jurkat cell adhesion to a surface immobilized ICAM 1 in shear flow , a ligand for leukocyte LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
PAF induced upregulation of CD11b and CD 18 on neutrophils and IL 1 beta increased surface expression of ICAM 1 and VCAM 1 on HUVEC . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The marker was not toxic for T cells or endothelial cells nor did it interfere with the expression or function of adhesion molecules on T cells ( LFA 1 , VLA 4 ) or endothelial cells ( ICAM 1 , VCAM 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Dominant negative effect of the lymphocyte function associated antigen 1 beta ( CD 18 ) cytoplasmic domain on leukocyte adhesion to ICAM 1 and fibronectin . ^^^ The CD4 / 18 , but not CD4 / 11a , chimera profoundly inhibited LFA 1 mediated cell adhesion to ICAM 1 , as well as cell spreading following cell adhesion . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NiSO 4 or pPD pretreatment of proliferative HaCaT cells enhanced T cell binding , which was abolished by neutralizing antibodies to ICAM 1 or CD 18 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To investigate the dynamics of activated leukocytes and the roles of CD 18 ICAM 1 pathway , we examined the effects of rat IL 8 and monoclonal antibodies ( mAbs ) against CD 18 and ICAM 1 on the behavior of leukocytes in microvessels of perfused rat lungs . ^^^ Anti CD 18 and anti ICAM 1 mAbs attenuated these changes . ^^^ These results suggest that unique features exist in the interaction between activated leukocytes and pulmonary microvessels , and that CD 18 ICAM 1 dependent capillary sequestration is one of the major mechanisms by which activated leukocytes accumulate in lungs . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Adhesion molecules identified were intercellular adhesion molecule 1 ( ICAM 1 ) , ICAM 2 , ICAM 3 , vascular cell adhesion molecule 1 ( VCAM 1 ) , platelet endothelial cell adhesion molecule 1 ( PECAM 1 ) , E selectin , P selectin , L selectin , lymphocyte function associated antigen 1 ( LFA 1 ) , and very late activation antigen 4 ( VLA 4 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The cell adhesion molecules CD 44 , CD 18 , L selectin , CD11a c as well as VLAalpha 4 , dramatically increase from the first week of long term marrow culture , while ICAM 1 and a new beta 2 cell adhesion molecule , alpha dbeta 2 , increased slightly from the third week on . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The structure of the two amino terminal domains of human ICAM 1 suggests how it functions as a rhinovirus receptor and as an LFA 1 integrin ligand . ^^^ ICAM 1 binds to leukocyte function associated antigen ( LFA 1 ) or macrophage 1 antigen ( Mac 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Mac 1 ( CD11b / CD18 ) , a monocytic counter receptor for intercellular adhesion molecule 1 ( ICAM 1 ) , that also binds to heparin , is present on Mono Mac 6 but not on U 937 cells , and may thus explain these differences in adhesion . ^^^ This suggests that two distinct endothelial ligands for Mac 1 , inducible ICAM 1 and carbohydrate decorated heparin like proteoglycan structures mediate monocytic cell interaction with ox LDL treated HUVEC . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In contrast , the expression of the PKA R ( G324D ) subunit has no effect on CD 40 mediated growth inhibition in M 12 cells , nor on CD 40 mediated induction of B 7 1 , CD 23 , Fas , ICAM 1 , or LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
METHODS : To investigate the mechanisms of intraglomerular leukocyte infiltration in IgA nephropathy ( IgA N ) and membranoproliferative glomerulonephritis type 1 ( MPGN 1 ) , we immunohistochemically examined the intraglomerular expression of leukocyte function associated antigen 1 ( LFA 1 , CD11a / CD18 ) , macrophage 1 ( Mac 1 , CD11b / CD18 ) and intercellular adhesion molecule 1 ( ICAM 1 , CD 54 ) together with glomerular deposition of C3c and fibrinogen . ^^^ The LFA 1 / ICAM 1 pathway may play an important role in glomerular leukocyte infiltration in IgA N , while the Mac 1 / complement pathway may be important in MPGN 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
B 7 expressing melanoma cells were excellent inducers of T cell proliferation , cytokine production , and cytolytic activity in allogeneic mixed lymphocyte cultures through a process dependent on the function of the T cell receptor as well as interactions between B 7 : CD 28 , CD 2 : LFA 3 , and LFA 1 : ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In the present study on idiopathic membranous glomerulonephritis ( MGN ) the expression of adhesion molecules ( ICAM 1 , VCAM 1 , PECAM 1 , E selectin , LFA 1 , Mac 1 ) was analyzed in different cellular compartments of the kidney using an indirect immunoperoxidase technique and monoclonal antibodies . ^^^ The number of LFA 1 expressing inflammatory cells in the interstitium correlated with peritubular capillary E selectin ( r = 0 . 8 , P < 0 . 001 ) and interstitial ICAM 1 ( r = 0 . 61 , P = 0 . 009 ) expression , but histological alterations did not correlate with the expression of adhesion molecules . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Thus our data suggest that the VAP 1 / VAP 1 receptor and ICAM 1 / LFA 1 pathways are important in the recruitment of T cells to HCC . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule 1 ( ICAM 1 ) is a ligand for the integrins lymphocyte function associated antigen 1 ( LFA 1 ) and complement receptor 3 ( Mac 1 ) , making it an important participant in many immune and inflammatory processes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Cord blood and mobilized blood CD34+ cells had a lower expression of VLA 2 , VLA 3 and VLA 5 and a higher expression of LFA 1 , ICAM 1 and L selectin than bone marrow CD34+ cells . ^^^ A small subset was VLA 2 , VLA 3 , ICAM 1 or Mac 1 positive . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Differential requirements for LFA 1 binding to ICAM 1 and LFA 1 mediated cell aggregation . ^^^ Our results demonstrate that activation of LFA 1 binding to ICAM 1 by CBR LFA 1 / 2 , in contrast to inside out signaling mechanisms , does not require protein kinase C activation or protein phosphatase 2A activity nor is it affected by agents that interfere with reorganization of the cytoskeleton . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The kinetics of lymphocyte function associated antigen 1 ( LFA 1 ) expression on spleen cells ( SPC ) and liver non parenchymal cells ( NPC ) , and intercellular adhesion molecule 1 ( ICAM 1 ) expression on hepatocytes ( HC ) was examined in acute liver injury mice induced by a DTH reaction to picryl chloride ( PCl ) . ^^^ The peak expression of LFA 1 on SPC was seen at 6 hr after eliciting liver injury , and then that of LFA 1 on NPC and ICAM 1 on HC appeared at 12 hr . ^^^ These results suggest that PCl elicitation in liver may trigger an increased expression of LFA 1 on SPC and NPC and ICAM 1 on HC . ^^^ LFA 1 / ICAM 1 interaction between liver infiltrating NPC , mainly including CD4+ and CD8+ T cells , and HC may be an essential step for the hepatocyte damage in PCl DTH liver injury . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We investigated the expression of adhesion molecules mediating cell cell [ intercellular adhesion molecule 1 ( ICAM 1 ) and lymphocyte function associated antigen 1 ( LFA 1 ) ] and cell matrix interactions [ very late antigen 4 ( VLA 4 ) , VLA 6 , and syndecan 1 ] in intestinal granulomas of mice infected with the parasite Schistosoma mansoni . ^^^ Up regulation of ICAM 1 , LFA 1 , and VLA 4 was seen in ileal and colonic granulomas , at both the acute ( 8 weeks postinfection ) and the chronic stage ( 13 16 weeks postinfection ) . ^^^ Organ related differences between hepatic and intestinal granulomas exist ( e . g . , granuloma volume ) , but these differences are not morphologically reflected in a differential expression of the adhesion molecules ICAM 1 , LFA 1 , and VLA 4 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Cellular interactions that lead to graft rejection were examined in a rat to mouse xenogeneic combination using species specific monoclonal antibodies ( mAbs ) against donor and recipient intercellular adhesion molecule 1 ( ICAM 1 ) and lymphocyte function associated antigen 1 ( LFA 1 ) molecules , respectively . ^^^ Although both mAbs displayed moderate blocking activity in an in vitro mixed lymphocyte response assay , strong suppression was observed when anti donor ( rat ) ICAM 1 mAb was combined with anti recipient ( mouse ) LFA 1 mAb . ^^^ Thus , 0 . 05 mg of anti mouse LFA 1 mAb and anti rat ICAM 1 mAb given on days 0 and 1 produced significant prolongation of graft survival over the control ( 51+ / 20 days vs . 10+ / 3 days , P < 0 . 0001 ) , but not when anti mouse ICAM 1 mAb was combined with anti mouse LFA 1 mAb ( 13+ / 3 days ) . ^^^ The binding assay showed that LFA 1 molecules on mouse T cells can bind immobilized rat ICAM 1 molecules . ^^^ These results suggest that rat ICAM 1 molecules on APCs can interact with mouse LFA 1 molecules on T cells across a species barrier and that this binding generates the consequent immune responses leading to rejection . mAb treatment against these adhesion molecules of recipient as well as donor is crucial for preventing rejection in a xenogeneic transplantation model . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule 1 ( ICAM 1 ) , leucocyte function associated antigen 1 ( LFA 1 ) and leucocyte infiltration in proliferative human glomerulonephritis . ^^^ The expression of the intercellular adhesion molecule 1 ( ICAM 1 ) , infiltrating cells positive for its ligand leucocyte adhesion molecule 1 ( LFA 1 ) , and the markers of total leucocytes ( CD45RB ) , T cells ( CD45RO ) , and monocytes / macrophages ( CD 68 ) were examined by an indirect immunoperoxidase method on renal biopsy specimens from 20 patients with mesangial proliferative glomerulonephritis IgA negative ( MesProGN ) and 20 with IgA nephropathy ( IgAN ) . ^^^ In both types of glomerulopathies a significant increase in the number of glomerular and interstitial LFA 1 positive cells correlated positively with the expression of ICAM 1 . ^^^ The strong correlation between expression of ICAM 1 and LFA 1 and between LFA 1 positive cells and immune cells makes these adhesion molecules useful markers of activity and suggest that they are involved in recruitment of leucocytes in the studied types of proliferative glomerulopathies . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
One of the most important interactions is between the integrin lymphocyte function associated antigen 1 ( LFA 1 ) on the T cell and intracellular adhesion molecule 1 ( ICAM 1 ) on an antigen presenting cell . ^^^ The use of peptide / MHC complexes and ICAM 1 on a supported lipid bilayer to stimulate T cells also indicates a major role for ICAM 1 / LFA 1 in T cell activation but , surprisingly , not for adhesion , as even in the absence of ICAM 1 the morphological changes and adhesive characteristics of an activated T cell are seen in this system . ^^^ We suggest that T cell antigen receptor mediated recognition of a very small number of MHC / peptide complexes could trigger LFA 1 / ICAM 1 clustering and avidity regulation , thus amplifying and stabilizing the production of second messengers . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Analysis of surface expression of the adhesion molecules LFA 1 , VLA 4 , and ICAM 1 show no significant differences in lymphocytes from alcohol consuming mice , and lymphocyte chemotaxis in vitro is also unaltered . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Antibodies against CD 69 , LFA 1 or ICAM 1 significantly inhibited the ability of T cells to activate macrophages by cell contact . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In this system , T cell adhesion and subsequent transmigration were blocked in both directions by monoclonal antibodies ( mAbs ) against lymphocyte function associated antigen 1 ( LFA 1 ) or intercellular adhesion molecule 1 ( ICAM 1 ) ( induced by interferon gamma [ IFN gamma ] treatment of epithelial cells ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Short term administration of monoclonal antibodies to ICAM 1 and leukocyte function associated antigen 1 ( LFA 1 ) results in an indefinite acceptance of cardiac allografts by induction of antigen specific tolerance , as evidenced by acceptance of the secondary skin allografts . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In fact , during the budding process , the HIV envelope captures a number of cell membrane proteins , including cytokine receptors such as IL 2R , adhesion molecules such as LFA 1 , ICAM 1 , 2 , HLA Class 1 and 2 , as well as cell lineage markers . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Using a culture system in which Abs directed to B cell surface markers are immobilized on the tissue culture plastic , we find that cell spreading can be mediated by a variety of Abs , including anti CD 44 , CD 23 , LFA 1 , VLA 4 , ICAM 1 , and Ig . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
ICAM 1 CD 18 interaction mediates neutrophil cytotoxicity through protease release . ^^^ This study examined interaction of the CD11a , CD11b , and CD 18 subunits of the beta 2 integrin with ICAM 1 , transfected into Chinese hamster ovarian ( CHO ) cells to avoid effects of other adhesion molecules . ^^^ Blockade with a monoclonal antibody ( MAb ) to ICAM 1 or MAbs to CD11a , CD11b , or CD 18 reduced PMN mediated cytotoxicity to baseline . ^^^ To examine the role of adhesion in cytotoxicity , we studied beta 2 integrin mediated PMN adhesion to ICAM 1 transfected CHO cells and found that MAbs for CD11a , CD11b , and CD 18 all abrogated PMN cytotoxicity despite disparate effects on adhesion . ^^^ This soluble ICAM 1 provoked elastase release , similar to PMA , which could be inhibited by MAbs to CD 18 but not MAbs to other beta 2 integrin subunits . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Major histocompatibility complex ( MHC ) class 1 , CD 29 , CD49d , and CD 51 were present on all stromal cell lines , MHC class 2 and CD 34 were consistently absent , and CD11a ( LFA 1 ) , CD 18 ( ICAM 1R ) , CD 54 ( ICAM 1 ) , CD 58 ( LFA 3 ) CD 56 ( N CAM ) , CD 106 ( 5 CAM ) , laminin , and collagen 4 were diversely expressed . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Lysis of syngeneic bystander cells was always FasL Fas dependent and required effector bystander contact and , in particular , an interaction between CTL LFA 1 and bystander ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Serial frozen sections of grafts and controls were stained by immunoperoxidase for rat MAC 1 , class 2 MHC , CD 2 , CD 4 , CD 8 , B cells , VLA 4 , LFA 1 , L selectin , ICAM 1 , and VCAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Monocyte and U 937 lymphocyte function associated antigen 1 ( CD11a / CD18 ) and macrophage antigen 1 ( CD11b / CD 18 , alpham / beta2 ) were both upregulated upon MP stimulation , but an increase in p 150 , 95 ( CD11c / CD18 ) , very late antigen 1 , or ICAM 1 expression was not observed . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The expression and distribution of the adhesion molecules ICAM 1 , VCAM 1 and E selectin by microvascular endothelial cells and tumor cells , together with their leukocyte receptors LFA 1 , VLA 4 and CLA respectively , were studied in 33 BCCs of different histological subtypes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The expression of ICAM 1 , ICAM 3 , LFA 3 , CD 2 , LFA 1 , VLA 4 , CD1a , VCAM 1 , CD 69 , and activated b 1 integrins were assessed by immunostaining . ^^^ An increased expression of LFA 1 , LFA 2 , ICAM 3 , VLA 4 , and activated b 1 integrins was observed in the cell infiltrate of actinic prurigo lesions and an up regulated expression of ICAM 1 was detected in keratinocytes from these specimens . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Eosinophil adhesion was E selectin , ICAM 1 and VCAM 1 dependent , as assessed by the inhibitory activity of ENA 2 and the ability of a mAb to the ICAM 1 ligand , CD 18 , and a mAb to the VCAM 1 ligand , VLA 4 , to attenuate adhesion . 5 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
This is followed by neutrophil adhesiveness to ICAM 1 via the LFA 1 antigen on its surface and the extravasation of neutrophils into the tissue . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
METHODS : The presence of adhesion molecules was investigated in iris biopsy specimens from 11 patients with uveitis and eight controls ( patients with primary open angle glaucoma ) immunohistochemically with a panel of monoclonal antibodies : LECAM ( CD 62L ) , ICAM 1 ( CD 54 ) , LFA 1 ( CD 11a / 18 ) , VCAM 1 ( CD 106 ) , VLA 4 ( CD 49d ) , and HECA 452 , a marker for high endothelial venules . ^^^ RESULTS : Positive staining for ICAM 1 , LFA 1 and VCAM 1 was found in the iris in a significantly higher number of uveitis patients than in controls . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
LFA 1 ( CD18 / CD11a ) and ICAM 1 ( CD 54 ) antigens were almost undetectable . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Here we investigated in parallel the role of beta 2 integrin family members , LFA 1 ( CD11a / CD18 ) and Mac 1 ( CD11b / CD18 ) on monocytes , and the immunoglobulin supergene family members , ICAM 1 and ICAM 2 on HSF and on human umbilical vein endothelial cells ( HUVEC ) , in monocyte migration through HSF and HUVEC monolayers . ^^^ Based on results of mAb blockade , ICAM 1 appeared to be the major ligand for LFA 1 dependent migration through the HSF . ^^^ In contrast , both ICAM 1 and ICAM 2 mediated LFA 1 dependent monocyte migration through HUVEC . ^^^ However , neither ICAM 1 nor ICAM 2 was required for Mac 1 dependent monocyte migration through either cell barrier , indicating that Mac 1 can utilize ligands distinct from ICAM 1 and ICAM 2 on HSF and on HUVEC during monocyte transmigration . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Expression of a soluble form of LFA 1 and demonstration of its binding activity with ICAM 1 . ^^^ The interaction between lymphocyte function associated antigen 1 ( LFA 1 ) and intercellular adhesion molecule 1 ( ICAM 1 ) is of importance in a number of cellular events , including antigen specific T cell activation and emigration of leukocytes into sites of inflammation . ^^^ We describe here the first use of a recombinant soluble form of human LFA 1 ( sLFA 1 ) for the measurement of the binding between LFA 1 and ICAM 1 . sLFA 1 has been successfully expressed and purified . ^^^ These results suggest that the soluble constructs would be useful tools for molecular analysis of ICAM 1 / LFA 1 interaction as well as in screening for ICAM 1 / LFA 1 antagonists . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Effects of blocking reagents ( injected intravenously ) to rat E , L and P selectins , beta 1 ( VLA 4 ) and beta 2 integrins ( LFA 1 and Mac 1 ) , ICAM 1 , and the cytokines TNFalpha , IL and IL 8 were assessed . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
After vaccination and challenge , CD4+ T cells from the pulmonary interstitium have reduced levels of ICAM 1 and LFA 1 expression , compared to wild type animals , which coincides with a reduced cohesiveness of foci . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Various adhesion molecules , including LFA 1 , ICAM 1 , CD 43 , CD 44 , P selectin , and E selectin , were up regulated . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The effect of Pseudomonas aeruginosa ( PA ) exotoxin A ( P ExA ) on CD 3 induced T cell activation was studied on the level of T cells ( proliferation , synthesis of interleukin ( IL ) 2 , expression of IL 2R complex , ICAM 1 , 2 and LFA 1 molecules ) , and on the level of monocytes ( expression of ICAM 1 , 2 , LFA 1 molecules , as well as FcRI and CD 14 receptors ) . ^^^ Action of P ExA on monocytes resulted in : ( 1 ) inhibition of the expression of ICAM 1 , 2 molecules and their ligand LFA 1 molecule ; ( 2 ) low expression of FcRI receptor ( a ligand for Fc part of CD 3 mAb ) ; and ( 3 ) inhibition ( over 90 % ) of the expression of CD 14 molecule . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
T lymphocyte populations , including CD 3 , CD 4 , CD 28 , and adhesion molecules ( L selectin , LFA 1 , VLA 4 , and ICAM 1 ) were measured by flow cytometry in a cross sectional study of 100 HIV infected and 49 HIV seronegative adults . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Surface plasmon resonance ( SPR ) was used to investigate and characterize the interaction between LFA 1 and sICAM 1 ( a soluble form of ICAM 1 ) . ^^^ Since the LFA 1 / ICAM 1 interaction is highly sensitive to the presence of metal cations , SPR was also used to probe the affinity of the metal binding sites . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In contrast , extensive mutagenesis analysis of ICAM 1 has shown that only residues on the GFC face interact with LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The ICAM 1 / LFA 1 interaction has been clearly demonstrated to play an active role in syncytium formation induced by human immunodeficiency virus type 1 ( HIV 1 ) . ^^^ Since it is known that a high affinity state of LFA 1 for ICAM 1 can be induced through conformational change , such a high affinity state may also contribute to the process of syncytium formation . ^^^ Inhibition could also be observed with specific blocking agents for the chemokine receptor CXCR 4 , as well as with soluble ICAM 1 , anti LFA 1 , anti ICAM 1 , and anti ICAM 2 blocking antibodies , indicating the requirement for the LFA 1 / ICAM interaction . ^^^ Based on the demonstration that an increase in ICAM 1 binding is induced by T cell activation , these data suggest an in vivo involvement of the high affinity state of LFA 1 in HIV 1 induced syncytium formation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In binding assays to immobilized ICAM 1 , the anti idiotype 10 anti LFA 1 was highly active in blocking 38C 13 cell adhesion . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To address this phenomenon , we used the air pouch ( AP ) model in control and infected Swiss webster mice , analyzing the cellular , tissue response and local expression of adhesion molecules [ CD 18 ( beta ( 2 ) chain ) , CD 44 , ICAm 1 ( CD 54 ) , L selectin ( CD62L ) , CD49d ( alpha ( 4 ) chain ) , LFA 1 ( CD 11 alpha ) ] . ^^^ In the AP wall of infected mice , a significant decrease in the expression of ICAM 1 and CD 44 in fibroblastic like cells and a reduction in the number of CD 18 and CD 11 alpha in migratory cells were observed . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
For ICAM 1 and ICAM 2 , this glutamic acid residue is located on a flat surface , complementary to the flat surface of the 1 domain of the integrin to which they bind , lymphocyte function associated antigen 1 ( LFA 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In addition , the response was independent of the two major lymphocyte adhesion receptors , LFA 1 and intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^ These findings suggest that serum and redox sensitive intracellular events regulate cellular responsiveness to MHC 2 triggered LFA 1 / ICAM 1 independent cell adhesion independently of differentiation . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Induction of donor specific tolerance to rat nerve allografts with portal venous donor alloantigen and anti ICAM 1 / LFA 1 monoclonal antibodies . ^^^ Recipients were treated with anti intercellular adhesion molecule 1 ( anti ICAM 1 ) and anti leukocyte function associated antigen 1 ( anti LFA 1 ) monoclonal antibodies ( mAbs ) , intraportally administered ultraviolet B ( UVB ) irradiated donor splenocytes , a combination of mAbs and UVB irradiated donor splenocytes , or no immunosuppression . ^^^ CONCLUSIONS : The combined use of mAbs directed against ICAM 1 and LFA 1 adhesion molecules with intraportally administered UVB modified donor antigen prevents nerve allograft rejection and synergistically improves nerve regeneration . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The expression of intercellular adhesion molecule 1 ( ICAM 1 ) , the ligand leucocyte function antigen 1 ( LFA 1 , CD11a ) , and complement receptor type 3 ( CR 3 , or Mac 1 , CD11b ) has been studied in murine kidneys acutely infected with the fatal malaria parasite Plasmodium berghei ANKA . ^^^ Thirty six kidney sections from five groups of C57BL / 6J mice on day 5 , 10 , 15 , and 20 post infection , and normal controls , were stained with monoclonal antibodies against ICAM 1 , LFA 1 , and Mac 1 . ^^^ Mesangial cells and inflammatory cells in the cortical tubulointerstitium showed positive staining for ICAM 1 , LFA 1 , and Mac 1 at the later stages of infection . ^^^ There were strong correlations between ICAM 1 expression on endothelial cells of glomerular / peritubular capillaries with inflammatory cells positive for LFA 1 and Mac 1 , which correlated with proteinuria . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Flow cytometric analysis revealed that 99 . 1 + / 0 . 9 % of the subcloned cells were positive for CD11b and CD 18 , both of which are ligands of the intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The fish oil diet decreased the level of expression of CD 2 , CD11a , CD 18 and CD 44 on the surface of freshly prepared lymphocytes and of CD 2 , CD11a , CD 18 , CD 54 ( intercellular adhesion molecule 1 ; ICAM 1 ) and CD62L ( L selectin ) on the surface of concanavalin A ( Con A ) stimulated lymphocytes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
This experiment was performed to study the roles of intercellular adhesion molecule 1 ( ICAM 1 ) , lymphocyte function associated antigen 1 ( LFA 1 ) , and another adhesion molecule , selectin , in the development of cationized antigen induced in situ immune complex glomerulonephritis ( CAICGN ) . ^^^ Treatment with monoclonal antibodies ( mAbs ) to both ICAM 1 and LFA 1 , as well as with a sulfatide , a ligand of L and P selectin , started within 2 days after CaIgG perfusion completely suppressed the development of proteinuria without affecting the glomerular deposition of immunoreactants . ^^^ Although sulfatide attenuated the PMN response 1 hour after CaIgG perfusion , ICAM 1 and LFA 1 mAb treatment did not alter PMN infiltration . ^^^ Treatment with ICAM 1 and LFA 1 mAbs started on day 5 , or treatment with sulfatide started on day 4 , after CaIgG perfusion did not affect albuminuria . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We recently demonstrated that different CD 45 monoclonal antibodies ( mAb ) are able to induce cellular aggregation in human peripheral blood mononuclear cells ( PBMC ) through LFA 1 / ICAM 1 interactions . ^^^ Importantly , pretreatment of T lymphocyte / monocyte mixtures with LFA 1 mAb and / or ICAM 1 mAb down regulated CD 45 mAb induced cAMP synthesis . ^^^ Based on the data presented , we propose that CD 45 signaling in T lymphocytes subsequently activates cAMP accumulation and PKA activation in monocytes via LFA 1 / ICAM 1 dependent cellular interactions . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In addition , in 19 cases the expression of adhesion molecules ( LFA 1 ( CD 18 ) , ICAM 1 ( CD 54 ) , alpha4beta1 integrin ( CD49d / CD29 ) , L selectin ( CD62L ) and CD 44 ) was studied . ^^^ Most LCL showed expression of LFA 1 , ICAM 1 and alpha4beta1 integrin . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Among these cell surface antigens , lymphocyte function associated antigen 1 ( LFA 1 ) and intercellular adhesion molecule 1 ( ICAM 1 ) were highly expressed on mononuclear cells in the cultures for forming preosteoclast like mononuclear cells . ^^^ The double detection of these two antigen molecules with osteoclast specific antigen and with calcitonin receptor , using a fluorescence activated cell sorter or autoradiography technique , revealed that LFA 1 and ICAM 1 were expressed on the preosteoclasts . ^^^ The expression of ICAM 1 was detected on both preosteoclasts and osteoclast like MNCs , whereas the expression of LFA 1 was restricted to preosteoclasts . ^^^ We designed a peptide with the sequence of the binding site of ICAM 1 against the ligand LFA 1 . ^^^ These results strongly suggest the involvement of an LFA 1 / ICAM 1 interaction in osteoclastogenesis . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
PAF was administered alone or in combination with either the PAF antagonist WEB 2086 ( 250 microg . kg 1 . min 1 ) , a monoclonal antibody ( MAb ) to CD 18 , or a MAb to intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^ CLI was performed in rats given either saline , WEB 2086 , anti CD 18 , or anti ICAM 1 MAb . ^^^ These increases were significantly attenuated by WEB 2086 , anti CD 18 MAb , and anti ICAM 1 MAb in both PAF and CLI treated rats . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule 1 ( ICAM 1 ) , a molecule bound to the cell surface , is a ligand for leukocyte function antigen 1 ( LFA 1 ) , and the ICAM 1 / LFA 1 system mediates various cell cell interactions involved in immunity . ^^^ Soluble ICAM 1 ( sICAM 1 ) is a circulating substance and binds with LFA 1 of leukocytes , thus , making leukocytes less available for binding with cell surface ICAM 1 on target cells . ^^^ These results suggest that both sICAM 1 and anti ICAM 1 monoclonal antibody act as immunosuppressive factors by inhibiting the ICAM 1 / LFA 1 system . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
PMN adherence directly stimulated increases in [ Ca2+ ] 1 in histamine or LPS treated EC . mAbs directed against leukocyte CD 18 or PECAM 1 , the leukocyte counter receptors for endothelial ICAM 1 and PECAM 1 , respectively , did not inhibit PMN induced EC activation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
METHODS : We assessed interstitial and glomerular leukocytes using an avidin biotin peroxidase method with monoclonal antibodies ( MAbs ) against leukocytes ( CD 45 ) , beta 2 integrin ( CD 18 ) , macrophages ( CD 14 ) , T cells ( CD 3 ) and T cell subsets ( CD 4 , CD 8 ) , intercellular adhesion molecule 1 ( ICAM 1 ) ( CD 54 ) , and HLA class 2 antigens . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies ( mAb ) against intercellular adhesion molecule 1 ( ICAM 1 ) and its CD 18 leukocyte integrin ligands have been shown to inhibit airway eosinophilia in animal models of asthma , although the role of this pathway in eosinophil epithelial adhesion is not fully understood . ^^^ To investigate the role in vitro of CD 18 and ICAM 1 , we measured adhesion of fluorescently labeled human eosinophils to normal human bronchial epithelial cell ( NHBEC ) monolayers pretreated for 24 h with culture medium ( low constitutive ICAM 1 ) or tumor necrosis factor alpha ( TNF alpha ; 1 ng / ml ) and interferon gamma ( IFN gamma ) ( 10 ng / ml ; increased ICAM 1 ) . ^^^ Stimulation of eosinophils with C5a ( 10 ( 7 ) M ) increased adhesion measured at 30 min to unactivated NHBEC from 11 . 4 + / 0 . 7 to 15 . 5 + / 0 . 4 % ( n = 4 ) , and this increase was CD18 / ICAM 1 independent , whereas phorbolmyristate acetate ( PMA ) ( 10 ( 8 ) M ) induced adhesion ( 20 . 7 + / 1 . 7 % ) was abolished by anti CD 18 and reduced by anti ICAM 1 . ^^^ In contrast , C5a and PMA induced adhesion to TNF alpha / IFN gamma activated NHBEC ( increased from 11 . 1 + / 1 . 3 % to 21 . 9 + / 1 . 0 % and 27 . 6 + / 1 . 9 % , respectively ) was CD 18 and ICAM 1 dependent . ^^^ In the absence of added chemoattractants , eosinophil adhesion to NHBEC increased with time and , at 120 min , was significantly greater ( P < 0 . 01 ) to activated NHBEC ( 37 . 3 + / 2 . 4 % , n = 5 ) than to unactivated monolayers ( 24 . 3 + / 1 . 9 % ) ; mAb against CD 18 or ICAM 1 abolished increased , but not basal , adhesion . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies against ICAM 1 and CD 18 attenuate cerebral vasospasm after experimental subarachnoid hemorrhage in rabbits . ^^^ Two of the key adhesion molecules responsible for the attachment of leukocytes to endothelial cells are intercellular adhesion molecule 1 ( ICAM 1 ) and the common beta chain of the integrin superfamily ( CD 18 ) . ^^^ This study examined the effects of monoclonal antibodies on ICAM 1 and the effects of CD 18 on cerebral vasospasm after SAH . ^^^ METHODS : A rabbit model of SAH was utilized to test the influence of intracisternally administered antibodies to ICAM 1 and CD 18 on cerebral vasospasm . ^^^ RESULTS : Treatment with antibodies to ICAM 1 or CD 18 inhibited vasospasm by 22 % and 27 % , respectively . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Migration of TAM2D2 cells through filters coated with the LFA 1 ligand ICAM 1 , induced by 1 ng / ml of the chemokine SDF 1 , was blocked by anti LFA 1 mAb and also abrogated by dominant negative ZAP 70 and piceatannol . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In this study , the expression of CD 44 during liver transplant rejection was compared with the expression of lymphocyte function associated antigen 1 ( LFA 1 ) and intercellular adhesion molecule 1 ( ICAM 1 ) , which play an essential role in cell interactions and the initiation of immune responses . ^^^ Immunohistochemical staining was used to investigate the expression of CD 44 , ICAM 1 , and LFA 1 . ^^^ CD 44 was strongly expressed in portal areas of the rejected liver , and LFA 1 and ICAM 1 were expressed mainly on sinusoids and hepatocytes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Fluorescence activated cell sorting ( FACS ) analysis was used to examine expression of cell adhesion molecules with antibodies to ICAM 1 and LFA 1 . ^^^ FACS analysis demonstrated that ICAM 1 and LFA 1 expression increased during EMIU , with ICAM 1 expression higher than that of LFA 1 . ^^^ Immunopathogenesis appeared to be mediated by cell expressing ICAM 1 and LFA 1 . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
This alteration in co stimulatory molecule expression was selective , because UV Mphi express equal levels of ICAM 1 and ICAM 3 , and increased levels of LFA 1 , relative to LC . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
LFA 1 , Mac 1 , CD 44 , ICAM 1 and P selectin were strongly expressed in the acute phase and to a lesser degree in the chronic phase of arthritis . ^^^ LFA 1 , Mac 1 , CD 44 , alpha 4 integrin , ICAM 1 and the selectins were found expressed on cells of the synovial infiltrate , LFA 1 , Mac 1 and ICAM 1 on the synovial lining layer , and VCAM 1 and P selectin on endothelial cells . ^^^ LFA 1 , Mac 1 , CD 44 , ICAM 1 and P selectin were strongly expressed in the acute phase and to a lesser degree in the chronic phase of arthritis . ^^^ LFA 1 , Mac 1 , CD 44 , alpha 4 integrin , ICAM 1 and the selectins were found expressed on cells of the synovial infiltrate , LFA 1 , Mac 1 and ICAM 1 on the synovial lining layer , and VCAM 1 and P selectin on endothelial cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The i . p . injection of LPS in vivo induces a systemic inflammatory reaction in a mouse model with generalized activation of both endothelial cells ( up regulation of adhesion molecules ICAM 1 , VCAM 1 , E selectin ) and neutrophils ( up regulation of Mac 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We measured production of monokines ( IL 6 and tumour necrosis factor alpha ( TNF alpha ) ) , active NO and reactive oxygen intermediates ( ROIs ) , as well as expression of several cell surface adhesion molecules ( Mac 1 , 2 and 3 , intercellular adhesion molecule 1 ( ICAM 1 ) and Fc gammaRII ) , by thioglycollate medium induced peritoneal macrophages of normoglycaemic and alloxan diabetic CBA / J mice ( blood glucose level in the range 300 or 500 mg / dl ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
On T cells the leukocyte integrin leukocyte function associated antigen 1 ( LFA 1 ) ( CD11a / CD18 ) can be induced to bind its ligand intercellular adhesion molecule 1 ( ICAM 1 ) ( CD 54 ) either by increasing the affinity of the receptor with Mg2+ and EGTA or by receptor clustering following activation with phorbol ester . ^^^ The existence of these two adhesion inducing pathways implies that alternative mechanisms might exist by which LFA 1 engages ICAM 1 . ^^^ The LFA 1 alpha subunit 1 domain contains a major binding site for ICAM 1 . ^^^ In this study we show that soluble LFA 1 1 domain blocks ICAM 1 binding of the high affinity Mg2+ induced form of LFA 1 but not the phorbol ester induced form . ^^^ In addition , the binding of Mg2+ activated LFA 1 to ICAM 1 is blocked by peptides covering the alpha 4 beta3 loop , the beta 3 alpha5 loop , and the alpha 5 helix of the 1 domain , whereas none of the peptides tested blocks phorbol ester mediated adhesion . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We also observed that target cells treated with NKI L 16 , an anti LFA 1 antibody known to increase the affinity of LFA 1 for ICAM 1 , were markedly more susceptible to infection with HIV 1 particles bearing on their surfaces large numbers of host derived ICAM 1 proteins . ^^^ Given that cellular activation of leukocytes is known to modify the conformational state of LFA 1 and induce ICAM 1 surface expression , it is tempting to speculate that activation of virus infected cells will lead to the production of HIV 1 particles bearing more host ICAM 1 on their surfaces and that such progeny virions will preferentially infect and replicate more efficiently in activated cells which are prevalent in lymphoid organs . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The increased binding induced by both cytokines and the TNF alpha induced sensitivity of SR 91 cells to NK 92 cell killing were abrogated by anti LFA 1 mAb as well as by a combination of antibodies against the three ligands of LFA 1 ( CD11a / CD18 ) , ICAM 1 ( CD 54 ) , ICAM 2 ( CD 102 ) and ICAM 3 ( CD 50 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
IL 18 induced heterotypic aggregation between KG 1 and Peer T cells , which was blocked by anti ICAM 1 and / or LFA 1 antibodies . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The ICAM 1 induced IL 2 production could efficiently be abrogated with monoclonal antibody ( mAb ) against ICAM 1 or LFA 1 , showing that the activation is dependent of a functional ICAM 1 LFA 1 pathway . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The most important adhesion molecule pairs are the selectins ( E , L and P ) , the immunoglobulins ICAM 1 and VCAM 1 , and the beta 2 and beta 1 integrins ( e . g . , LFA 1 and VLA 4 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
An immunohistological study revealed that cells infiltrating the dermis expressed LFA 1 , whereas ICAM 1 , the ligand of LFA 1 , was detected on endothelial cells and keratinocytes . ^^^ These results suggest that tonsillar lymphocytes may have an affinity for the skin of PPP expressing adhesion molecules such as LFA 1 , ICAM 1 , and E selectin . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We investigated the expression of some adhesion molecules ( E and L selectin , VLA 4 , ICAM 1 , PECAM 1 , VCAM 1 and CD 18 and CD11c chains of beta 2 integrins ) on endothelial and inflammatory cells by immunohistochemistry on cryostat sections of erythema nodosum lesions taken from 15 patients with BD and 12 patients with erythema nodosum of unknown cause . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
By extensive mutagenic analysis of the inserted domain ( 1 domain ) of the alpha chain ( CD11a ) of the leukocyte function associated antigen 1 ( LFA 1 ) , we have defined a putative binding surface for intercellular adhesion molecules 1 and 2 ( ICAM 1 and 2 ) . ^^^ This analysis showed that individually mutating Leu 205 or Glu 241 to alanine completely abolished LFA 1 binding to ICAM 1 or 2 without affecting 1 domain structure , as assayed by antibody binding . ^^^ Mutating Thr 243 to alanine also had a profound effect on LFA 1 binding to ICAM 1 and 2 , as seen by complete loss of binding to ICAM 1 and a significant reduction ( 70 % decrease ) in binding to ICAM 2 . ^^^ Mutating Glu 146 to alanine reduced LFA 1 binding to ICAM 1 or 2 by 70 % , and mutating His 264 or Glu 293 to alanine reduced binding to ICAM 1 or 2 by about 30 40 % . ^^^ Using these data , we have generated a model of the interface between the LFA 1 1 domain and residues in the first domain of ICAM 1 that have been shown to be critical for this interaction . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
METHODS : Binding and non binding monoclonal antibodies ( mAb ) were radiolabeled and injected into wild type mice and mice deficient of P selectin , CD 18 , or ICAM 1 . ^^^ An equivalence of PECAM 1 expression was observed in unstimulated tissues among wild type mice and mice that are genetically deficient in either CD 18 , ICAM 1 , or P selectin . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
HgCl 2 was previously found to enhance expression of LFA 1 , ICAM 1 and CD 134 ( OX 40 ) on T cells in BN rats . ^^^ Ex vivo experiments demonstrated that HgCl 2 exposure of BN rats , but not of Lewis rats , significantly enhances PMA [ phorbol 12 myristate 13 acetate ] induced lymphocyte aggregation , mediated by LFA 1 and ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Loss of surface ICAM 1 on antisense KM 102 transfectants did not significantly affect adhesion to LFA 1 bearing JY hematopoietic cells . ^^^ However , KM 102 ICAM 1 overexpressors demonstrated enhanced binding ( 2 . 5 fold ) to phorbol ester treated , but not untreated , LFA 1 bearing JY cells . ^^^ These findings suggest that while ICAM 1 is not required for basal adhesion between stromal and hematopoietic cells , stromal ICAM 1 may contribute to stromal : leukemic cellular interaction when bound to the phorbol ester dependent high avidity state of hematopoietic LFA 1 . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Myocardial ischemia reperfusion injury in CD 18 and ICAM 1 deficient mice . ^^^ We investigated the effects of MI / R in wild type , CD 18 , and ICAM 1 deficient ( / ) mice . ^^^ Wild type ( n = 6 ) , CD 18 / ( n = 6 ) , and ICAM 1 / ( n = 6 ) mice were subjected to 30 min of myocardial ischemia and 120 min of reperfusion to determine the extent of PMN infiltration and myocardial cell necrosis . ^^^ In contrast , the extent of myocardial infarction was significantly ( P < 0 . 05 ) reduced in the CD 18 ( 19 . 3 + / 5 . 1 % ) and ICAM 1 ( 17 . 9 + / 3 . 2 % ) deficient mice . ^^^ Similarly , PMN infiltration into the ischemic reperfused myocardium was attenuated by 54 % in the CD 18 / mice and by 32 % in ICAM 1 / mice compared with wild type hearts . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The major leukocyte adhesion molecules , such as leukocyte function associated antigen 1 ( LFA 1 ) , intercellular adhesion molecule 1 ( ICAM 1 ) , and CD 44 , retain their biological functions when expressed on the virion surface , and have been shown to increase virus cell interaction , enhance virus infectivity , and extend the host cell range of the virus . ^^^ Hence , LFA 1 , ICAM 1 , and other cellular adhesion molecules are involved in different stages of HIV 1 infection and profoundly affect HIV 1 neutralization by virus specific antibodies . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Shedding of L selectin and PECAM 1 and upregulation of Mac 1 and ICAM 1 on neutrophils in RSV bronchiolitis . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
LFA 1 interaction with ICAM 1 and ICAM 2 regulates Th 2 cytokine production . ^^^ By comparison , blocking CD28 / B7 costimulation inhibited IL 4 and IL 5 ( Th 2 cytokine ) production , whereas blocking LFA 1 / ICAM 1 or LFA 1 / ICAM 2 led to a significant increase ( 15 to 40 fold ) of Th 2 cytokines . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Treatment with mAbs against the adhesion molecules leukocyte function associated antigen 1 ( LFA 1 ) and intercellular adhesion molecule 1 ( ICAM 1 ) ( both known for their involvement in PMN endothelial cell interaction ) inhibits the development of skin vascular lesions . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Although it is clear that LFA 1 must undergo affinity or avidity changes to bind its cellular ligand ICAM 1 , the intracellular signaling pathways involved are not well characterized . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Since HLE has been shown to bind to Mac 1 , and ICAM 1 plays a key role during the recruitment and the activation of leukocytes at inflamed sites , we investigated the capacity of HLE to cleave ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Furthermore , a downregulation of the endothelial expression of the adhesion molecules ICAM 1 at the target site and LFA 1 on lymphocytes could also contribute to the absence of inflammatory cells in the neuraxis . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We applied an established microassay in which constitutive and tumour necrosis factor alpha ( TNF alpha ) induced binding of mouse T lymphoma cells ( TK 1 ) to mouse endothelioma ( eEND . 2 ) cells is mediated by the interaction of intercellular adhesion molecule 1 ( ICAM 1 ) and vascular cell adhesion molecule 1 ( VCAM 1 ) on EC with their counter receptors the LFA 1 heterodimer ( CD11a / CD18 ) and VLA 4 on T cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
METHODS : The expression of very late activation antigen 4 ( VLA 4 ) and vascular cell adhesion molecule 1 , lymphocyte function associated antigen 1 ( LFA 1 ) , and intercellular adhesion molecule 1 ( ICAM 1 ) was analyzed immunocytologically on lymphocytes and monocytes from blood and CSF of 23 patients and 11 healthy control subjects . ^^^ RESULTS : After treatment , the mean proportions of VLA 4 , LFA 1 , and ICAM 1 on blood lymphocytes ( p < 0 . 0003 , p < 0 . 00001 , p < 0 . 01 ) and monocytes ( p < 0 . 0001 , p < 0 . 0002 , p < 0 . 007 ) of 23 patients decreased . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Improvement in nerve regeneration by monoclonal antibodies to ICAM 1 and LFA 1 in allogeneic mice . ^^^ We examined whether giving monoclonal antibodies ( MoAb ) to intercellular adhesion molecule 1 ( ICAM 1 ) and leucocyte function associated antigen ( LFA 1 ) , which have important roles in the initial stage of rejection after allografts , can improve nerve regeneration in allogeneic mice . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We have also found that CD 18 , ICAM 1 , or P selectin deficient mice are less sensitive to the ulcerogenic effects of orally administered indomethacin . ^^^ We conclude from these studies that indomethacin activates NFKB ( possibly via TNF synthesis ) in gastric microvascular endothelial cells , thereby enhancing surface expression of ICAM 1 which binds the CD 18 on polymorphonuclear leukocytes ( PMNs ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Expression of ICAM 1 , VCAM 1 , and LFA 1 in adenocarcinoma of the lung with observations on the expression of these adhesion molecules in non neoplastic lung tissue . ^^^ Intercellular adhesion molecule ( ICAM 1 ) , vascular cell adhesion molecule ( VCAM 1 ) , and the lymphocyte function associated antigen ( LFA 1 ) are cell adhesion molecules thought to play an important role in the complex process of airway inflammation and tumor cell growth . ^^^ The aim of this study was to examine the distribution of ICAM 1 , VCAM 1 , and LFA 1 in adenocarcinoma of lung and in major cellular compartments of non neoplastic lung tissue . ^^^ Upregulation of ICAM 1 in pulmonary adenocarcinoma might foster binding by LFA 1 bearing lymphocytes , with a possible impact on the vulnerability of tumor cells to host defense mechanisms . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
THP 1wt adherence to ICAM 1 was LPS dose related , time dependent , and inhibited by antibodies to either CD 14 or leukocyte function associated antigen ( LFA ) 1 , but was independent of any change in the number of surface expressed LFA 1 molecules . ^^^ This converts low avidity LFA 1 into an active form capable of increased binding to ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We found that activation of B cells with the mitogenic stimuli induced an increased avidity of LFA 1 for its ligand , intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Liver metastases are enhanced in homozygous deletionally mutant ICAM 1 or LFA 1 mice . ^^^ The interaction of intercellular adhesion molecule 1 ( ICAM 1 ) with lymphocyte function antigen 1 ( LFA 1 ) is important for the adhesion of leukocytes , monocytes and lymphocytes to endothelial cells in vitro and in vivo . ^^^ In order to explore the role of the ICAM 1 / LFA 1 interaction in liver metastases , we utilized homozygous deletionally mutant ( gene knockout ) mice for ICAM 1 or LFA 1 which had been derived from the C57BL6 / J background . ^^^ LFA 1 deficient mice developed 13 times the number of metastases compared to wild type controls and ICAM 1 deficient mice developed 7 times that number [ 13 . 5 ( n = 17 ) vs 1 . 0 ( n = 19 ) and 36 ( n = 10 ) vs 5 . 0 ( n = 16 ) , P values of 0 . 0003 and 0 . 0002 by Wilcoxon Rank Sum Test , respectively ] . ^^^ Liver metastases of the B 16 melanoma are markedly enhanced in ICAM 1 null and LFA 1 null mice . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The present study evaluated the role of MoAb to LFA 1 ' s ligand , intercellular adhesion molecule 1 ( ICAM 1 ) in GVHD . ^^^ Saline treated SBTx GVHD and sham operated control animals were compared to animal groups treated with MoAb to ICAM 1 or MoAb to ICAM 1 and LFA 1 . ^^^ Treatment with MoAb to both ICAM 1 and LFA 1 appeared to have a synergistic beneficial effect on GVHD ( range 19 29 days , P < 0 . 001 vs untreated animals ) . ^^^ MoAb to ICAM 1 alone or in combination with MoAb to LFA 1 ameliorates GHVD after SBTx and prolongs survival . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Strong cellular upregulation of the adhesion molecules ICAM 1 , LFA 1 , syndecan 1 , VLA 4 , but not VLA 6 is seen , both in acute and chronic granulomas . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Increased adhesion of TMM cells was impaired neither by antibodies to VLA 4 , LFA 1 , or Mac 1 nor by antibodies to their counter receptors VCAM 1 or ICAM 1 , suggesting that adhesion molecules distinct from VCAM 1 or ICAM 1 are involved in enhanced adhesiveness of the fibroblasts to myeloid leukemic cells . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We evaluated the relative contribution of ICAM 1 and ICAM 2 , known ligands on endothelium for LFA 1 and Mac 1 , in spontaneous neutrophil ( PMN ) transendothelial migration ( TEM ) across IL 1 activated HUVEC monolayers or TEM induced by C5a or IL 8 across unstimulated HUVEC grown on polycarbonate filters . ^^^ Adhesion blocking mAb to ICAM 1 [ R6 . 5 F ( ab ) 2 ] or ICAM 2 [ CBR IC2 / 2 F ( ab ) 2 ] tended to inhibit TEM under each condition but , in general , inhibition was significant only with both ICAM 1 and ICAM 2 blockade . mAb to LFA 1 partially inhibited migration to C5a or IL 8 across unstimulated HUVEC and inhibition was not altered by additional treatment of HUVEC with mAbs to ICAM 1 and 2 . ^^^ In contrast , with IL 1 HUVEC , mAb to ICAM 1 significantly inhibited this LFA 1 independent TEM . mAb to Mac 1 alone partially inhibited TEM and , when combined with mAb to LFA 1 , migration was almost completely blocked with all TEM conditions tested . ^^^ The contribution of alternate ligands for Mac 1 in mediating Mac 1 dependent but ICAM 1 / 2 independent C5a induced TEM was examined using anti LFA 1 treated PMN and anti ICAM treated resting HUVEC . ^^^ The results indicate that : ( 1 ) LFA 1 mediates PMN TEM primarily by interaction with ICAM 1 and ICAM 2 ; ( 2 ) ICAM 2 may function in concert with ICAM 1 in this role , especially on unstimulated endothelium , and ( 3 ) Mac 1 on PMN also plays a major role in TEM and can utilize yet to be identified ligands distinct from ICAM 1 or 2 , especially on unstimulated endothelium . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The expression of intercellular adhesion molecule 1 ( ICAM 1 ) by endothelial cells is important for the regulation of adhesion and transendothelial migration of a variety of leukocytes that express the integrins lymphocyte function associated antigen 1 ( LFA 1 ) and / or Mac 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule 1 ( ICAM 1 ) is a member of the immunoglobulin superfamily and a ligand for lymphocyte function associated antigen 1 alpha ( LFA 1 alpha ) . ^^^ Clinical and experimental investigations have demonstrated a close relationship between the ICAM 1 / LFA 1 alpha pathway and the pathogenesis of asthmatic responses and acute respiratory distress syndrome . ^^^ Further progress in research on the antagonism of ICAM 1 and LFA 1 alpha may provide new therapeutic approaches to inflammatory lung diseases . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Integrins support cell function by providing co stimulatory second signals that are a precondition for full cell activation first described for ICAM 1 binding to LFA 1 in lymphocytes . ^^^ Expression of the LFA 1 counter receptor ICAM 1 on endothelial cells occurs only at the site of inflammation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
This effect of SOD treatment after endotoxin infusion was associated with a significant inhibition of glomerular monocyte influx and a significant inhibition of adhesion molecule expression ( glomerular ICAM 1 and VCAM 1 and leukocyte LFA 1 and VLA 4 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We have investigated the contribution of TCR , CD 28 , and LFA 1 signaling to T cell cytoskeletal polarization by assaying the response of an Ag specific Th 1 clone toward a panel of transfected APCs expressing MHC class 2 alone or in combination with ICAM 1 or B 7 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Immunoprecipitation studies showed markedly elevated ICAM 1 expression but > 10 fold reduction in LFA 1 expression on MDDC compared with monocytes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Further , we show that it is likely to be upregulation of ICAM 1 and its subsequent engagement of LFA 1 rather than loss of cell surface sialic acid that in part regulates access to CD 86 and other costimulatory molecules . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Furthermore , using KL / 4 cells ( LFA 1 expressing ) and Jurkat cells ( VLA 4 expressing ) , we demonstrated specific LFA 1 / ICAM 1 and VLA 4 / VCAM 1 mediated interactions within glomerular and tubulointerstitial compartments . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Selective eosinophil transendothelial migration triggered by eotaxin via modulation of Mac 1 / ICAM 1 and VLA 4 / VCAM 1 interactions . ^^^ Taken together , our results suggest that eotaxin induced eosinophil transendothelial migration in vivo and in vitro relies on Mac 1 / ICAM 1 and VLA 4NCAM 1 interactions , the latter ones becoming more relevant at later time points of the eotaxin induced recruitment process . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Atherosclerosis and inflammation mononuclear cell recruitment and adhesion molecules with reference to the implication of ICAM 1 / LFA 1 pathway in atherogenesis . ^^^ Among these adhesion molecules , ICAM 1 , a protein of the Ig superfamily , and one of the ligands for LFA 1 have been suggested to play an important role in atherogenesis . ^^^ Further immunohistochemical studies have demonstrated that LFA 1 is expressed by more than 85 % of macrophages in the lesions , and their presence therefore may point toward the involvement of the LFA 1 / ICAM 1 receptor ligand pathway in the recruitment of mononuclear cells in the lesions . ^^^ In order to verify this hypothesis , systemic administration of blocking antibodies was attempted ; injection of anti ICAM 1 / LFA 1 monoclonal antibodies significantly reduced macrophage adherence and their emigration into the intima . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Expression of the adhesion molecules ICAM 1 , VCAM 1 , LFA 1 and VLA 4 in the skin is modulated in progressing stages of chronic venous insufficiency . ^^^ In this study , we determined how the expression of ICAM 1 / VCAM 1 on endothelial cells and their ligands LFA 1 / VLA 4 on leukocytes is modulated in skin of progressing stages of chronic venous insufficiency . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In this study , the requirement of cell adhesion molecules , CD11a / CD18 ( LFA 1 ) , CD 54 ( ICAM 1 ) , CD49d / CD29 ( VLA 4 ) , and CD 106 ( VCAM 1 ) , for T cell infiltration into tumors and antitumor function was investigated . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Additionally , the expression of activation markers such as B 7 1 , B 7 2 , CD 71 , ICAM 1 , and LFA 1 on Mac1+ fraction in both spleen and lung and the concentration of TNFalpha in bronchoalveolar lavage fluid were not significantly suppressed . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule 1 ( ICAM 1 ) is expressed abnormally on the bile duct epithelium during the course of primary biliary cirrhosis ( PBC ) , but the importance of ICAM 1 and its lymphocyte function associated antigen 1 ( LFA 1 ) receptor during the course of nonsuppurative destructive cholangitis ( NSDC ) has not been defined . ^^^ We also determined the effects of anti ICAM 1 and anti LFA 1 treatments on NSDC , intrahepatic lymphokine production , and the homing of lymphocytes to the livers of GVHD mice . ^^^ Treatment with anti ICAM 1 ( but not anti LFA 1 ) decreased both the mean grades of portal inflammation ( P = . 003 ) and NSDC ( P = . 002 ) lesions compared with control immunoglobulin G ( IgG ) treatments . ^^^ Combined treatment with anti ICAM 1 and anti LFA 1 caused a further decrease in the amount of portal inflammation and bile duct damage compared with anti ICAM 1 , alone ( P = . 02 ) . ^^^ Neither anti ICAM 1 nor anti LFA 1 prevented lymphocytes from homing to the liver . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Immunohistochemistry was performed using the APAAP method and directing specific mouse anti human monoclonal antibodies against ICAM 1 ( CD 54 ) , VCAM 1 ( CD 106 ) , E selectin ( CD62E ) , alpha LFA 1 ( CD11a ) , alpha Mac 1 ( CD11b ) , alpha p150 / 95 ( CD11c ) and the beta 2 integrin chain ( CD 18 ) . ^^^ The expression of ICAM 1 , VCAM 1 and E selectin in human myocardium is variable and little is known about the expression of LFA 1 and Mac 1 during allograft rejection . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Although both subsets infiltrated the pancreas and elicited multiple adhesion receptors ( peripheral lymph node addressin , mucosal addressin cell adhesion molecule 1 , LFA 1 , ICAM 1 , and VCAM 1 ) on vascular endothelium , entry / accumulation of Th 1 cells was more rapid than that of Th 2 cells , and only Th 1 cells induced diabetes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
It has been well documented that LMP 1 causes B cells to up regulate adhesion molecules , such as LFA 1 and ICAM 1 , and coactivation molecules , such as B 7 1 and CD 23 , as well as to activate NF kappaB . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Surprisingly , up regulation of Fas , CD 23 , and ICAM 1 was partially independent , and up regulation of LFA 1 was completely independent , of CD 40 induced NF kappaB activation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
A Ca2+ binding cyclic peptide derived from the alpha subunit of LFA 1 : inhibitor of ICAM 1 / LFA 1 mediated T cell adhesion . ^^^ This peptide has been shown to inhibit ICAM 1 LFA 1 mediated T cell adhesion . ^^^ There is evidence that alterations in the conformation of LFA 1 upon binding to divalent cations cause LFA 1 to bind to ICAM 1 . ^^^ The peptide can inhibit T cell adhesion by directly binding to ICAM 1 or by disrupting the interaction of the alpha and beta subunits of LFA 1 protein . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
FDCs could capture the viral particles on their surface ; however , the binding of HIV 1 to FDCs was strongly inhibited by the presence of anti CD 54 ( ICAM 1 ) monoclonal antibody ( MAb ) and anti CD11a ( LFA 1 ) MAb , suggesting that the adhesion molecules play an important role in the interaction between HIV 1 and FDCs . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Using mice deficient for LFA 1 , CD 44 , and ICAM 1 , we examined the role of these adhesion molecules in NK1 . 1+TCR alpha beta+ ( NKT ) cell development . ^^^ Although no defect in NKT cell development was observed in CD 44 / and ICAM 1 / mice , a dramatic reduction of liver NKT cells was observed in LFA 1 / mice . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The ICAM 1 induced enhancement we observed has differential sensitivity to LFA 1 blockade , depending on the T cell subsets and cytokine evaluated . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
A vaccinia virus based RNA expression system enabled high level cytoplasmic expression of RNA aptamers directed against the intracellular domain of the beta 2 integrin LFA 1 , a transmembrane protein that mediates cell adhesion to intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Using flow cytometry , we observed a marked increase in the expression of MHC class 1 and 2 , M CSF receptor and adhesion molecules LFA 1 , LFA 3 , ICAM 1 ( P < 0 . 05 ) on HPS+ splenic monomacrophages compared to HPS , which was independent of their HIV status . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
However , it is unclear whether the binding of LFA 1 to its ligand , ICAM 1 , functions through intercellular adhesion alone , resulting in an augmentation of the TCR signal , or involves an additional LFA 1 mediated cellular signal transduction pathway . ^^^ To determine whether LFA 1 / ICAM 1 interactions were simply enhancing the presentation of low concentrations of specific MHC / peptide complexes generated from exogenously added peptide , we transfected cells with class 2 that is covalently coupled to peptide , alone or in combination with ICAM 1 . ^^^ LFA 1 / ICAM 1 interactions are not required for effective conjugate formation and TCR engagement because presentation of class II / peptide complexes in the absence of ICAM 1 does induce up regulation of CD 25 and CD 69 . ^^^ Thus , high numbers of engaged TCR can not compensate for the lack of LFA 1 / ICAM 1 interactions in the activation of naive CD4+ T cells . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Liver CD4+NKT cells expressed ICAM 1 and high levels of LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
LFA 1 binding to ICAM 1 can enhance TCR dependent proliferation of T cells , but it has been difficult to distinguish contributions from increased adhesion , and thus TCR occupancy , versus costimulatory signaling . ^^^ Thus , under conditions in which ICAM 1 provides costimulation of proliferation , LFA 1 ligation activates some of the same signaling pathways as does CD 28 ligation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
However , studies using blocking antibodies against the common beta 2 integrin subunit on the PMN , the counterligand for ICAM 1 ( CD 18 ) , have demonstrated equivocal efficacy . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Wild type SHIP and a phosphatase deficient mutant SHIP were overexpressed in the murine myeloid cell line , DA ER , and the effects on LFA 1 mediated cell adhesion to ICAM 1 ( CD 54 ) were tested . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
DXM also directly down regulates the expression of ICAM 1 that is the high affinity ligand of LFA 1 , by which the self aggregation of MC 9 is inhibited . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
B CLL lymphocytes and normal B cells expressed CD 23 but this molecule was down regulated following transendothelial migration , whereas the expression of VLA 4 , ICAM 1 , LFA 1 and CD 44 was unchanged . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Our group has recently demonstrated that an antibody mediated induction of the high affinity state of LFA 1 for ICAM 1 renders target T cells more prone to HIV 1 dependent syncytium formation and infection by ICAM 1 bearing virions . ^^^ To further substantiate these results , we made use of mutant cell lines expressing LFA 1 in either a low ( parental HPB ALL and HAmut ) or a high affinity state for ICAM 1 ( HAP 4 ) and compared syncytium formation and virus infection . ^^^ Cells expressing the activated form of LFA 1 were found to be more susceptible to HIV 1 induced syncytium formation and to infection by ICAM 1 bearing HIV 1 particles . ^^^ The observed increase was solely due to the LFA 1 activation state because it was abrogated by anti LFA 1 or anti ICAM 1 antibodies and not due to variations in surface expression of LFA 1 , CD 4 , or the chemokine coreceptor CXCR 4 . ^^^ However , a linear relation was seen between the level of CXCR 4 surface expression and susceptibility to syncytium formation / virus infection when ICAM 1 LFA 1 interaction was either absent ( i . e . , infection with ICAM 1 negative virions ) or abrogated ( treatment with anti LFA 1 or anti ICAM 1 antibodies ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The adhesion induced by either IFN gamma or TNF alpha was inhibited by monoclonal antibodies against ICAM 1 or lymphocyte function associated antigen 1 ( LFA 1 ) . ^^^ These results indicate that IFN gamma and TNF alpha induce the expression of ICAM 1 on parenchymal hepatocytes and that the LFA 1 ICAM 1 pathway plays an important role in the interaction between hepatocytes and neutrophils or lymphocytes . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The expression of LFA 1 , ICAM 1 , CD 80 and CD 86 molecules in lupus patients : implication for immunotherapy . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Here , Minke Binnerts and Yvette van Kooyk discuss evidence from mutational and crystallographic studies showing that binding of LFA 1 to its ligands intercellular adhesion molecule 1 ( ICAM 1 ) , 2 and 3 might be structurally distinct . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Although MMF did not interfere with the synthesis of T cell adhesion ligands , the binding activity of lymphocytic leucocyte function associated antigen 1 ( LFA 1 ) , very late antigen 4 ( VLA 4 ) and PSGL 1 ( P selectin glycoprotein ligand 1 ) to immobilized intercellular adhesion molecule 1 ( ICAM 1 ) , vascular cell adhesion molecule 1 ( VCAM 1 ) and P selectin was impaired . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Exposure of endothelial cells to oxidized low density lipoprotein ( oxLDL ) induces upregulation of ICAM 1 and increases adhesion of monocytic cells expressing Mac 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Similarly , anti CD 28 injections affected expression of the adhesion molecules LFA 1 and ICAM 1 by splenocytes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
CT treatment did not affect the expression of B 7 1 , B 7 2 , ICAM 1 , LFA 1 or MHC class 2 on , or the internalization of OVA into A 20 HL cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In a retrospective immunohistochemical study based on 27 patients with stage 4 follicle center lymphoma ( FCL ) the expression of CD44standard ( CD44s ) , LFA 1 ( CD11a , CD 18 ) , VLA 4 ( CD49d , CD 29 ) and ICAM 1 ( CD 54 ) was analysed on lymphoma cells in bone marrow infiltrates . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
CD 147 monoclonal antibodies induce homotypic cell aggregation of monocytic cell line U 937 via LFA 1 / ICAM 1 pathway . ^^^ The CD 147 mAb induced cell aggregation was inhibited by leucocyte function antigen 1 ( LFA 1 ) and intercellular adhesion molecule 1 ( ICAM 1 ) mAbs . ^^^ However , the expression of LFA 1 and ICAM 1 molecules on U 937 was not altered by CD 147 mAb treatment . ^^^ We therefore propose that the binding of CD 147 mAb to CD 147 molecule , which mimics the natural ligand binding , may generate intracellular signals that activate LFA 1 / ICAM 1 intercellular adhesion pathway . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The main AM studied either by immunohistochemistry on lymph node sections or by flow cytometry in blood and bone marrow specimens are L selectin , CD11a / CD18 ( LFA 1 ) , CD 54 ( ICAM 1 ) , CD 44 ( HCAM ) , CD11c / CD18 ( gp150 / 95 ) , and CD49d / CD29 ( VLA 4 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Human T cell leukaemia / lymphoma virus type 1 syncytium formation is regulated in a cell specific manner by ICAM 1 , ICAM 3 and VCAM 1 and can be inhibited by antibodies to integrin beta 2 or beta 7 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Ubiquitin proteasome system is involved in induction of LFA 1 / ICAM 1 dependent adhesion of HL 60 cells . ^^^ Membrane permeable proteasome inhibitors , lactacystin ( LC ) and N acetyl Leu Leu norleucinal ( ALLN ) , but not calpain inhibitor Z Leu leucinal ( ZLL ) , prevented LFA 1 / ICAM 1 dependent cellular adhesion of TPA stimulated HL 60 cells . ^^^ They suppressed the increase in the avidity of LFA 1 to ICAM 1 without changing the expression of these molecules . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
CONCLUSIONS : These observations indicate that exposure of HT 29 cells to bile acids or ammonia induces CD11b / CD18 ( Mac 1 ) dependent but CD11a / CD18 ( LFA 1 ) independent neutrophil adhesion to intestinal epithelial cells , and ICAM 1 is unlikely involved in the interactions . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We exploited this setup to investigate which part of the cell adhesion protein LFA 1 forms a connection to the cytoskeleton after binding to its ligand ICAM 1 . ^^^ Interestingly , single particle tracking measurements using beads coated with the ligand ICAM 1 indicate that this mutant form of LFA 1 does not move freely within the cell membrane , suggesting that LFA 1 is still connected to the cytoskeleton network . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The peptide hinders T cell adhesion by inhibiting the leukocyte function associated antigen 1 ( LFA 1 , CD11a / CD18 ) interaction with ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
While the virus particle gained several molecules when passed through the different cell lines ( e . g . , ICAM 3 , LFA 1 , ICAM 1 , or MHC class 2 ) , BZ 167 never incorporated significant levels of CXCR 4 , CCR 5 , or CCR 3 into its envelope even though some or all of the cell lines in which it was grown expressed them . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Many of the infiltrating glomerular M phi expressed lymphocyte function associated antigen 1 ( LFA 1 ) and very late antigen 4 ( VLA 4 ) , which are ligands for ICAM 1 and VCAM 1 , respectively . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Differential Th 1 and Th 2 cell regulation of murine cardiac allograft acceptance by blocking cell adhesion of ICAM 1 / LFA 1 and VCAM 1 / VLA 4 . ^^^ Each combination of anti VCAM 1 plus anti VLA 4 mAbs ( 100 microg each / day , i . p . ) or anti ICAM 1 plus anti LFA 1 mAbs ( 50 microg each / day , i . p . ) was administered for 5 days . ^^^ Th 2 cytokines ( IL 4 and IL 10 ) were markedly enhanced and Th 1 cytokines ( IFN gamma and IL 2 ) were suppressed in recipients treated with anti ICAM 1 mAb plus anti LFA 1 mAb , while poor Th 2 cytokine expression allowed persistent Th 1 cytokine expression in recipient mice with anti VCAM 1 mAb plus anti VLA 4 mAb treatment . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In particular , attention has been drawn to the role exerted by those cytokine ( TNF alpha and IL 1 beta ) and adhesion molecules ( LFA 1 , Mac 1 , ICAM 1 ) that regulate interactions between leukocyte and endothelial cells leading to gastric microvessels occlusion and ischaemic / hypoxic endothelial epithelial cell damage . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
METHODS : Reverse transcription polymerase chain reaction and immunohistochemistry were used to detect and localize expression of adhesion molecules : P selectin , intercellular adhesion molecule 1 ( ICAM 1 ) , and lymphocyte function associated antigen 1 ( LFA 1 ) . ^^^ ICAM 1 and P selectin protein expression was increased within the muscularis microvasculature , and ICAM 1 and LFA 1 were expressed on infiltrating cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Pressure delivery of AS ICAM 1 ODN with LFA 1 mAb reduces reperfusion injury in cardiac allografts . ^^^ BACKGROUND : The goal of this study is to determine the effects of ex vivo hyperbaric pressure administration of AS ICAM 1 ODN and systemic anti LFA 1 mAb treatment on reperfusion injury in the rat cardiac allograft model . ^^^ Combination of pressure administration of AS ICAM 1 ODN and anti LFA 1 mAb decreased wet weight ratios ( 77 . 1+ / 0 . 93 % , p < 0 . 05 vs saline ) , myeloperoxidase activity ( 2 . 88+ / 0 . 44 , p < 0 . 01 vs saline ) , and contraction band necrosis ( 6 . 75+ / 5 . 67 % , p < 0 . 05 vs saline ) . ^^^ CONCLUSIONS : Ex vivo pressure mediated delivery of AS ICAM 1 ODN decreases ICAM 1 protein expression , reduces reperfusion injury in rodent cardiac allografts , and is more effective than anti LFA 1 mAb treatment alone . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The LFA 1 adhesion molecule is involved in cell adhesion events of leukocytes through binding to ICAM 1 , ICAM 2 and ICAM 3 . ^^^ We demonstrated that LFA 1 co stimulation under suboptimal concentrations of anti CD 3 monoclonal antibodies resulted in high , intermediate and weak proliferation of T cells on ICAM 1 , 2 , and 3 , respectively , which correlates with the distinct affinities of LFA 1 for these ligands . ^^^ Interestingly , we observed that LFA 1 / ICAM 1 co stimulation of T cells resulted in high production of the Th 2 cytokine IL 10 compared to ICAM 2 or ICAM 3 co stimulation . ^^^ In contrast , ICAM 2 and ICAM 3 induced a much stronger secretion of the Th 1 cytokine TNF alpha compared to LFA 1 / ICAM 1 induced co stimulation , despite the lower proliferation rate . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Furthermore , most of interstitial foam cells were positive for both leukocyte function associated antigen 1 ( LFA 1 ) and intercellular adhesion molecule 1 ( ICAM 1 ) , but not for ICAM 3 ( the third ligand for LFA 1 ) . ^^^ By contrast , most of infiltrating nonfoamy M phi s were positive for ICAM 3 and LFA 1 , however , ICAM 1 was observed on only some of them . ^^^ The accumulation of foam cells , which have characteristics of mature M phi , may be related to ICAM 1 as a ligand of LFA 1 , whereas infiltration of nonfoamy M phi s has a close relationship with ICAM 3 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In the present study the changes expression of adhesion molecules CD11a , CD 18 ( LFA 1 ) , CD 54 ( ICAM 1 ) and L selectin ( CD62L ) and VLA 4 ( CD49d ) were analysed by flow cytometry . ^^^ The most significant finding was a significant decrease of ICAM 1 expression on monocytes and CD49d on monocytes and lymphocytes as well as an increase of L selectin expression on monocytes after treatment with DSCG , without any associated effect on CD11a and CD 18 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies directed against the adhesion glycoproteins CD 18 , P selectin , or ICAM 1 showed similar patterns of protection against the I / R induced inflammatory responses in WKY and SHR . ^^^ The enhanced albumin extravasation noted in postischemic venules of SHR was prevented by immunoneutralization of either CD 18 on leukocytes or ICAM 1 on endothelial cells . ^^^ These results suggest that , whereas long term arterial hypertension does not significantly modify the leukocyte and platelet recruitment normally elicited in venules by I / R , it does result in an exaggerated albumin leakage response , which is mediated by an interaction between beta ( 2 ) ( CD 18 ) integrins on leukocytes and ICAM 1 on endothelial cells . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Time course of expression of ICAM 1 and LFA 1 in rat lung allografts . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Preincubation of interleukin 4 ( IL 4 ) stimulated human umbilical vein endothelial cells ( HUVECs ) with anti CD 31 monoclonal antibodies ( MoAbs ) enhanced eosinophil adhesion to the IL 4 stimulated HUVECs , and the endothelial CD 31 induced enhancement of eosinophil adhesion to IL 4 stimulated HUVECs was prevented by anti vascular cell adhesion molecule 1 ( VCAM 1 ) MoAb and anti very late activation antigen 4 ( VLA 4 ) MoAb , but not by anti intercellular adhesion molecule 1 ( ICAM 1 ) MoAb , anti lymphocyte function associated antigen 1 ( LFA 1 ) MoAb , anti P selectin MoAb , or anti E selectin MoAb . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The trafficking of leukocytes through tissues is supported by an interaction between the beta 2 ( CD 18 ) integrins CD11a / CD18 ( LFA 1 ) and CD11b / CD18 ( Mac 1 ) and their ligand ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Pretreatment of T cells with rolipram or cAMP analogues inhibited the IL 15 stimulated increases in proliferation , expression of cell surface molecules CD 69 , ICAM 1 , and LFA 1 , and release of TNF alpha from macrophages . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
A matrix metalloproteinase ( MMP ) inhibitor and monoclonal antibodies to lymphocyte function antigen 1 ( LFA 1 ) or intercellular adhesion molecule 1 ( ICAM 1 ) , but not to very late antigen 4 or to vascular cell adhesion molecule 1 significantly inhibited the transendothelial migration of stimulated leukocytes , suggesting that this phenomenon involves the LFA 1 / ICAM 1 interaction and MMP . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Ten patients with multiple sclerosis and treated with interferon beta1b ( IFN beta1b ) were followed up for 1 year with quantitation of serum VCAM 1 and ICAM 1 levels , mean fluorescence intensity of HLA DR , VLA 4 , CD11a , and CD 18 on peripheral blood monocytes and lymphocytes , and adhesion of peripheral blood monocytes and CD45+ cells on endothelial cell monolayers . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
High glucose induces enhanced monocyte adhesion to valvular endothelial cells via a mechanism involving ICAM 1 , VCAM 1 and CD 18 . ^^^ Using a battery of specific monoclonal antibodies molecules it was found that the increased adhesion of monocytes to VEC grown in high glucose was specifically inhibited ( p < 0 . 05 ) by anti ICAM 1 , anti VCAM 1 and anti CD 18 monoclonal antibodies . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We determined whether intercellular adhesion molecule 1 ( ICAM 1 ) mediated adhesion of LFA 1 , lacking parts of its cytoplasmic tails , is regulated through receptor diffusion / clustering and / or by altered ligand binding affinity . ^^^ Surprisingly , whereas these mutants showed a clustered cell surface distribution of LFA 1 , the ligand binding affinity as measured by titration of soluble ligand ICAM 1 remained unaltered . ^^^ The notion that redistribution of LFA 1 does not alter ligand binding affinity is further supported by the finding that disruption of the cytoskeleton by cytochalasin D did not alter the binding affinity nor adhesion to ICAM 1 of these mutants . ^^^ Most cytoplasmic deletion mutants that spontaneously bound ICAM 1 were not capable to spread on ICAM 1 , demonstrating that on these mutants LFA 1 is not coupled to the actin cytoskeleton . ^^^ From these data we conclude that LFA 1 mediated cell adhesion to ICAM 1 is predominantly regulated by receptor clustering and that affinity alterations do not necessarily coincide with strong ICAM 1 binding . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
When indomethacin was administered to mice deficient in CD 18 , intercellular adhesion molecule 1 ( ICAM 1 ) , or P selectin , there were significant decreases in lesion scores compared with their C57BL / 6 controls . ^^^ In addition , mucosal permeabilities were found to be significantly lower in CD 18 or ICAM 1 deficient mice observed at 12 hours . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Together these observations suggest that short exposure of HT 29 cells to TNF alpha and histamine induces CD11b / CD18 ( Mac 1 ) dependent but CD11a / CD18 ( LFA 1 ) independent neutrophil adhesion to intestinal epithelial cells , and ICAM 1 is not likely to be involved in the interactions . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
PATIENTS AND METHODS : LFA 1 and VLA 4 mediated adhesion to ICAM 1 and VCAM 1 coated beads was investigated using freshly isolated monocytes and T lymphocytes from patients with homozygous FH , heterozygous FH ( before and after cholesterol lowering treatment ) , and from controls . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Adhesion of IL 12 activated PBL was totally abolished by anti lymphocyte associated function antigen type 1 ( anti LFA 1 ) antibodies but was unaffected by anti alphaebeta 7 antibodies indicating that adhesion of the CLA positive subset is mediated via LFA 1 interaction with intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Additionally , we studied the effect of local IFN alpha expression on the cellular adhesion molecules , VLA 4 , Mac 1 , ICAM 1 , and L selectin in K 562 cells , and human umbilical endothelial vein cells . ^^^ K 562 cells transduced with the IFN alpha gene expressed a significantly elevated level of VLA 4 , Mac 1 , and ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We analyzed the role of ICAM 1 , CD 18 , CD11a , CD11b , and CD11c during episodes of rejection or infection in patients after lung transplantation and compared the results to episodes without apparent complication . ^^^ ICAM 1 , CD 18 , CD11a , CD11b , and CD11c expressions were detected immunocytochemically on alveolar macrophages . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
CONCLUSION : AS ODN hyperbaric transfection proved highly efficient , effective at ICAM 1 blockade , and induced cardiac allograft tolerance when combined with LFA 1 monoclonal antibody . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NK cell lysis of breast cancer targets is variable and is partially dependent on recognition through ICAM 1 and CD 18 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
METHODS : Monoclonal antibodies to CD 4 , CD 8 , Ia ( k ) , Ia ( d ) , lymphocyte function associated antigen 1 ( LFA 1 ) , and intercellular adhesion molecule 1 ( ICAM 1 ) were used in this study . ^^^ RESULTS : Proliferative response of IRBP sensitized lymph node cells was inhibited strongly by anti CD 4 or anti LFA 1 monoclonal antibody and moderately by anti Ia ( k ) or anti ICAM 1 monoclonal antibody . ^^^ CONCLUSIONS : CD 4 , Ia , LFA 1 , and ICAM 1 molecules play important roles in the antigen specific immune response of lymphocytes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
ICAM 1 binds to leukocyte function associated antigen ( LFA 1 ) or macrophage 1 antigen ( Mac 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Peptides derived from ICAM 1 and LFA 1 modulate T cell adhesion and immune function in a mixed lymphocyte culture . ^^^ METHODS : Short peptides representing counter receptor contact domains of LFA 1 and ICAM 1 were examined for their ability to inhibit T cell adhesion and T cell function . ^^^ RESULTS : Peptides encompassing amino acids Q 1 C21 and D 26 K50 of ICAM 1 , I 237 I261 and G 441 G466 of the LFA 1 alpha subunit , and D 134 Q159 of the LFA 1 beta subunit inhibited LFA 1 / ICAM 1 dependent adhesion in a phorbol 12 , 13 dibutyrate induced model of tonsil T cell homotypic adhesion . ^^^ CONCLUSIONS : Our data suggest that these peptides or their derivatives may be useful as therapeutic modulators of LFA 1 / ICAM 1 interaction during organ transplants . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We have investigated the intercellular cross talk between lymphocyte function associated antigen 1 ( LFA 1 ) and its counter receptor , intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^ Repeated systemic administration of anti cell adhesion molecule ( anti CAM ) monoclonal antibodies ( MoAbs ) , i . e . anti LFA 1 and anti intracellular adhesion molecule 1 ( anti ICAM 1 ) , in AK 5 tumour bearing animals enhanced tumour growth and caused delayed regression . ^^^ Marked reduction in the lymphokine activated killer ( LAK ) activity of NK cells was also observed after in vitro blocking of LFA 1 and ICAM 1 molecules . ^^^ These results underline the importance of ligand binding between LFA 1 and ICAM 1 , which could provide co stimulation for the effector cell functions via signalling events to induce cytotoxicity , apoptosis and cytokine production , resulting in the efficient regression of AK 5 tumour in syngeneic hosts . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
After the administration of mAbs directed against LFA 1 , ICAM 1 , murine VCAM 1 , VLA 4 , MadCAM or alpha 4 , beta 7 integrin prior to the cell transfer we could demonstrate a significant decrease of donor lymphocyte adherence in islets ( P < 0 . 01 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Blocking ICAM 1 ( CD 54 ) and LFA 1 ( CD11a ) inhibits experimental allergic conjunctivitis . ^^^ We investigated the role of ICAM 1 and LFA 1 in a previously described experimental model of ragweed ( Rw ) induced allergic conjunctivitis . ^^^ SWR / J mice were treated intraperitoneally 6 and 1 h prior to topical challenge with Rw with injections of anti ICAM 1 monoclonal antibody ( mAb ) , anti LFA 1 mAb , both anti ICAM 1 and anti LFA mAbs , or rat IgG . ^^^ Blocking ICAM 1 or LFA 1 reduced the clinical signs of allergic conjunctivitis . ^^^ Treatment with anti ICAM 1 or anti LFA 1 mAbs also significantly inhibited cellular infiltration into the conjunctiva . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
After zymosan activated serum stimulation , LFA 1 deficient neutrophils demonstrated activation , evidenced by up regulation of surface Mac 1 , but did not show increased adhesion to purified ICAM 1 or endothelial cells , similar to CD 18 deficient neutrophils . ^^^ Genetic deficiencies demonstrate that both LFA 1 and Mac 1 contribute to adhesion of neutrophils to endothelial cells and ICAM 1 , but adhesion through LFA 1 overshadows the contribution from Mac 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Evaluation of functional nerve recovery shows that allogeneic nerve graft treated with ICAM 1 and LFA 1 mAbs can be good alternative to syngeneic graft . ^^^ The objective of the study is to establish recovery results of tibial nerve defects reconstructed using allogeneic and xenogeneic graft , in host immunosuppressed with Intercellular Adhesion Molecule 1 ( ICAM 1 ) and Lymphocyte Function Antigen 1 ( LFA 1 ) monoclonal antibodies ( mAbs ) . ^^^ The main study included forty eight rats allocated to the following groups ( n = 12 / group ) : 1 ) Allograft without treatment as control group . 2 ) Allograft with intraperitoneal ICAM 1 and LFA 1 mAbs treatment . 3 ) Allograft preserved in Belzers ' solution including ICAM 1 mAbs plus standard intraperitoneal treatment . 4 ) Syngraft as benchmark . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
A pair of correspondent adhesion molecules : LFA 1 ( CD11aCD18 ) and ICAM 1 ( CD 54 ) was shown to be involved in autoimmune insulitis in animal models . ^^^ Anti LFA 1 or anti ICAM 1 monoclonal antibodies administered in vivo had a very strong preventive effect on the development of spontaneous diabetes with a marked reduction of insulitis . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To our knowledge , this is the first demonstration that SDF 1 expressed on vascular endothelium is crucial for translating rolling adhesion of CD 34 ( + ) progenitors into firm adhesion by increasing the adhesiveness of the integrins VLA 4 and LFA 1 to their respective endothelial ligands , VCAM 1 and ICAM 1 . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We anlayzed the role of the ICAM 1 and leukocyte function associated antigen 1 ( LFA 1 ) interaction in human T cell alloreactivity in vitro . ^^^ RESULTS : Alloantigen induced T cell proliferation and cytotoxic T lymphocyte activity were markedly inhibited by antibodies to ICAM 1 and LFA 1 . ^^^ Inhibition was not associated with altered cell surface expression of receptors such as CD 3 , CD 4 , ICAM 1 , LFA 1 , CD 25 , and HLA DR however , these antibodies did impede the ability of generation of functionally active T cells . ^^^ CONCLUSIONS : These data demonstrate that blockade of ICAM 1 / LFA 1 binding at the time of allorecognition potently blocks initial T cell effector functions that could be due to lack of effective T cell / APC engagement . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In this paper we report the discovery and characterization of ( R ) 5 ( 4 bromobenzyl ) 3 ( 3 , 5 dichlorophenyl ) 1 , 5 dimethylimidazolidine 2 , 4 dione ( BIRT 377 ) , an orally bioavailable small molecule that interacts specifically with LFA 1 via noncovalent binding to the CD11a chain and prevents LFA 1 from binding to its ligand , ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Development of a cell free binding assay for rat ICAM 1 / LFA 1 interactions using a novel anti rat LFA 1 monoclonal antibody and comparison with a cell based assay . ^^^ The importance of the interaction between lymphocyte function associated antigen 1 ( LFA 1 ) and intercellular adhesion molecule 1 ( ICAM 1 ) in the progression of inflammatory responses in vivo has been demonstrated mainly in rats . ^^^ The present study was undertaken to develop binding assays suitable for measuring the rat ICAM 1 / LFA 1 interaction in vitro . ^^^ We first examined binding of rat T lymphoma FTL 43 cells , which express LFA 1 , to immobilized rat ICAM 1 . ^^^ Although FTL 43 cells bound avidly to immobilized ICAM 1 and the binding was abolished with anti LFA 1 monoclonal antibodies ( mAbs ) , the binding was not completely inhibited by most anti ICAM 1 mAbs . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We investigated the induction of adhesion molecules such as L selectin , E selectin , ICAM 1 , VCAM 1 and Mac 1 on Schwann cells by proinflammatory cytokines . ^^^ E selectin , an adhesion molecule classically upregulated during inflammation , as well as Mac 1 , a ligand for ICAM 1 , were not expressed on human Schwann cells at basal condition or after treatment with cytokines . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The first is to allow leukocyte infiltration of respiratory mucosa , since they express LFA 1 and Mac 1 , which are ligands of ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Staining intensities for major histocompatibility complex ( MHC ) class 2 molecules and for the adhesion molecules leukocyte function associated antigen 1 ( LFA 1 ) and intercellular adhesion molecule 1 ( ICAM 1 ) were significantly decreased in ramified microglia 5 days after exposure to ACM . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We have used papiovirus transformed pigtailed macaque B cells as immunogen to generate murine MAbs against macaque adhesion molecules including ICAM 1 , VCAM 1 , and LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Previously , we have shown that Ag presentation in the context of LFA 1 costimulation by fibroblasts transfected with class 2 and ICAM 1 ( ProAd ICAM ) can drive naive CD 4 positive T cells into cell cycle , but these T cells die by apoptosis 4 5 days after stimulation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
ICAM 1 ( CD 54 ) , the ligand for LFA 1 and Mac 1 , is up regulated during inflammatory reaction on the activated vascular endothelium . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Here we investigated major adhesion molecules as predisposition factors via common polymorphisms in or in the vicinity of the candidate genes ICAM 1 , e selectin , PLAUR , CD11b , and CD 18 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In comparison , stimulation of T cells in the absence of lymphocyte function associated antigen ( LFA ) 1 intercellular adhesion molecule ( ICAM ) 1 interaction shifted the dose response curve by a factor of 10 , whereas absence of both CD 2 CD48 and LFA 1 ICAM 1 interactions shifted the response by a factor of approximately 100 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The chemokine induced T cell adhesion could be mimicked by expression of small G proteins , fully activated H RasV 12 , or H RasV12Y40C mutant , which selectively binds to PI 3 kinase , in T cells , inducing activated form of LFA 1alpha and LFA 1 dependent adhesion to ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Our recent finding that resistance to lymphoma cell metastasis in intercellular adhesion molecule 1 ( ICAM 1 ) deficient mice was manifested after homing suggested that the mechanism could involve the capacity of ICAM 1 to induce , via leukocyte function associated antigen 1 ( LFA 1 ) signaling , the expression of new genes necessary for migration and survival of lymphoma cells after homing . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We found that an HLA DR+ subclone ( HEL DR+ ) expresses LFA 1 , LFA 3 , ICAM 1 , ICAM 3 , but neither CD 80 nor CD 86 on the surface . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
ICAM 1 interactions with the beta 2 integrins CD11a / CD18 ( LFA 1 ) and CD11b / CD18 ( MAC 1 ) on the surface of leukocytes are important for their transendothelial migration to sites of inflammation and their function as costimulatory molecules for T cell activation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Taken together , these results suggest that lymphocytes infiltrating GD thyroid induce proliferation of GD thyrocyte by the cellular adhesion to thyrocytes via ICAM 1 / LFA 1 , which may lead to the development of a goiter . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Low affinity LFA 1 / ICAM 3 interactions augment LFA 1 / ICAM 1 mediated T cell adhesion and signaling by redistribution of LFA 1 . ^^^ Here we investigated the role of ICAM 3 in supporting LFA 1 mediated ICAM 1 binding and subsequently cell signaling . ^^^ We observed that although ICAM 3 binds poorly to LFA 1 expressed on resting T cells , it specifically facilitates and increases LFA 1 mediated adhesion to the high affinity ligand of LFA 1 , ICAM 1 . ^^^ We demonstrate that low affinity binding of LFA 1 to ICAM 3 together with ICAM 1 alters the cell surface distribution of LFA 1 dramatically , inducing large clusters of LFA 1 that facilitate ICAM 1 binding after LFA 1 activation . ^^^ We found that LFA 1 mediated ICAM 1 cell cell interactions such as T cell proliferation greatly depend on low affinity LFA 1 / ICAM 3 interactions that enhance stable LFA 1 / ICAM 1 cell cell contact . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Adhesion molecules identified were intercellular adhesion molecule 1 ( ICAM 1 ) , ICAM 2 , ICAM 3 , vascular cell adhesion molecule 1 ( VCAM 1 ) , platelet endothelial cell adhesion molecule 1 ( PECAM 1 ) , E selectin , P selectin , L selectin , lymphocyte function associated antigen 1 ( LFA 1 ) , very late activation antigen 4 ( VLA 4 ) , Mac 1 ( CD18 / CD11b ) , and gp 150 , 95 ( CD18 / CD11c ) . ^^^ Concurrent analysis of leukocyte receptors indicated a preferential use of VLA 4 / VCAM 1 and LFA 1 / ICAM 1 at the adventitia and Mac 1 / ICAM 1 at the intima media junction . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The purpose of this study was to evaluate the augmented protection when anti rat ICAM 1 and anti rat LFA 1 antibodies are combined with sulfatide in the ischemia reperfusion model of rat skin flaps . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Effect of anti CD11b ( alphaM MAC 1 ) and anti CD 54 ( ICAM 1 ) monoclonal antibodies on indomethacin induced chronic ileitis in rats . ^^^ This study investigated the effect of monoclonal antibodies against CD 54 ( ICAM 1 ) and CD11b ( alphaM chain of MAC 1 ) on intestinal inflammation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
CAS 1609 also attenuated both P selectin and ICAM 1 expression on mesenteric venules and decreased CD 18 expression on isolated leukocytes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The interaction between lymphocyte function associated antigen 1 ( LFA 1 ) and intercellular adhesion molecule 1 ( ICAM 1 ) is an essential process for hepatocyte ( HC ) damage . ^^^ Expression of LFA 1 on NPC and ICAM 1 on HC were examined by FACScan and ELISA , respectively . ^^^ In this case , an increased expression of both LFA 1 on NPC and ICAM 1 on HC was also observed . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
These results suggested that nedocromil suppressed the influx of eosinophils to inflammatory lesions by inhibiting not only the expression of the Mac 1 on eosinophils and of E selectin and ICAM 1 molecules on HUVEC , but also the eosinophil chemotactic activities . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The T cells expressed lymphocyte function associated antigen ( LFA ) 1 and CD 2 , and the synovial cells expressed intracellular adhesion molecule ( ICAM ) 1 and LFA 3 , suggesting possible interactions via LFA 1 / ICAM 1 and CD2 / LFA 3 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
RESULTS : Peripheral blood eosinophils constitutively expressed low levels of ICAM 1 and ICAM 2 ( < 10 FMI ) , moderate levels ( 10 50 FMI ) of CD 29 and L selectin , and high levels ( > 50 FMI ) of ICAM 3 , LFA 1 , and Mac 1 . ^^^ Calcium ionophore ( 1 microM ) significantly increased Mac 1 and ICAM 1 expression at 6 and 24 h . ^^^ CONCLUSIONS : These findings suggest that some AM , such as ICAM 1 , Mac 1 , and L selectin , may be involved in adhesion during eosinophil activation and that glucocorticoids may prevent airway inflammation by regulating the expression of AM in eosinophils . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
MAC 1 ( CD11b / 18 ) positive cells adhering to the surface of ICAM 1 / artery grafts were significantly less as identified by en face immunofluorescence , and these positive cells were more abundant in intimal lesions of artery grafts in wild type mice . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
LFA 1 and ICAM 1 dependent homotypic aggregation of human thymocytes induced by JL 1 engagement . ^^^ It required an intact cytoskeleton and the interaction between leucocyte function associated antigen 1 ( LFA 1 ) and intercellular adhesion molecule 1 ( ICAM 1 ) since it was blocked by cytochalasin B and D , and mAb against LFA 1 and ICAM 1 which are known to be involved in the aggregation of thymocytes . ^^^ In summary , JL 1 is a cell surface molecule that induces homotypic adhesion mediated by the LFA 1 and ICAM 1 interaction and cytoskeletal reorganization . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The accumulation of dendritic cells in the lung is impaired in CD 18 / but not in ICAM 1 / mutant mice . ^^^ To identify factors facilitating their emigration to the lung , mutant mice deficient in E selectin , P selectin , E / P selectin , ICAM 1 , or CD 18 and their respective controls were examined . ^^^ Increased numbers of arteries exhibited continuous luminal VCAM 1 staining in both CD 18 / and ICAM 1 / mutants . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Studies with mAbs in vivo and cellular studies in vitro suggest that PMN induced injury of the cardiac myocyte involve Mac 1 adhesion to myocyte ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To identify the intracellular signals which increase the adhesiveness of leukocyte function associated antigen 1 ( LFA 1 ) , we established an assay system for activation dependent adhesion through LFA 1 / intercellular adhesion molecule 1 ICAM 1 using mouse lymphoid cells reconstituted with human LFA 1 and then introduced constitutively active forms of signaling molecules . ^^^ We found that the phorbol myristate acetate ( PMA ) responsive protein kinase C ( PKC ) isotypes ( alpha , betaI , betaII , and delta ) or phosphatidylinositol 3 OH kinase ( PI 3 kinase ) itself activated LFA 1 to bind ICAM 1 . ^^^ Rap 1 induced changes in the conformation and affinity of LFA 1 and , interestingly , caused marked LFA 1 / ICAM 1 mediated cell aggregation . ^^^ Furthermore , a dominant negative form of Rap 1 ( Rap1N17 ) inhibited T cell receptor mediated LFA 1 activation in Jurkat T cells and LFA 1 / ICAM 1 dependent cell aggregation upon differentiation of HL 60 cells into macrophages , suggesting that Rap 1 is critically involved in physiological processes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Though levels of total LFA 1 and ICAMs as well as the active form of LFA 1 on T cell blasts were comparable in the presence and absence of LOK , clustering of active LFA 1 detected by binding of soluble ICAM 1 was accelerated in the absence of LOK . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
MAC 1 ( CD11b / 18 ) positive cells that adhered to the surface of vein grafts in ICAM 1 / mice were significantly less as identified with en face immunofluorescence , and these positive cells were more abundant in the intimal lesions of vein grafts in wild type mice . ^^^ When tumor necrosis factor alpha stimulated SMCs were incubated with mouse spleen leukocytes , the number of cells that adhered to ICAM 1 / SMCs was significantly lower than the number that adhered to ICAM 1 + / + SMCs , which was markedly blocked through pretreatment of leukocytes with the anti MAC 1 antibody . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Roles of alpha ( 4 ) integrins / VCAM 1 and LFA 1 / ICAM 1 in the binding and transendothelial migration of T lymphocytes and T lymphoblasts across high endothelial venules . ^^^ Antibodies to alpha ( 4 ) integrins , VCAM 1 or LFA 1 maximally inhibited T lymphocyte adhesion by 40 50 % , whereas antibodies to ICAM 1 were less effective ( < 20 % inhibition ) . ^^^ Antibodies to alpha ( 4 ) integrins , VCAM 1 , LFA 1 or ICAM 1 had little effect on T lymphoblast adhesion ( maxima of 10 30 % inhibition ) and T lymphoblasts transmigrated normally in the presence of either alpha ( 4 ) integrin or LFA 1 antibodies . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
It has been reported that fibrinogen may act as a bridging ligand , binding to intercellular adhesion molecule 1 ( ICAM 1 ) on human umbilical vein endothelial cells and to Mac 1 on THP 1 cells ( a monocytic cell line ) to increase adhesion . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Different expression of ICAM 1 and LFA 1 alpha by peripheral leukocytes during respiratory syncytial virus and influenza virus infection in young children . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Increased infectivity of HIV type 1 particles bound to cell surface and solid phase ICAM 1 and VCAM 1 through acquired adhesion molecules LFA 1 and VLA 4 . ^^^ In this study we have examined the effect on infectivity of immobilization or margination of HIV 1 through acquired integrins LFA 1 and VLA 4 onto nonsusceptible cells and solid phase adhesion ligands ( ICAM 1 and VCAM 1 , respectively ) . ^^^ LFA 1 and VLA 4 mediated HIV 1 binding was supported by ICAM 1 and VCAM 1 immunoglobulin Fc chimeras , respectively . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In the presence of different concentrations of budesonide ( 0 . 1 100 nM ) , we tested : a ) eosinophil migration induced by C5a through HBEC monolayers ; b ) ICAM 1 expression on HBECs , stimulated with C5a and c ) LFA 1 and Mac 1 expression on eosinophils , stimulated with C5a or with ah CD 23 mabs plus GM CSF . ^^^ Preincubation of the cells with different concentrations of budesonide was also effective in down regulating the C5a induced ICAM 1 expression on HBECs and the ah CD 23 and GM CSF induced LFA 1 and Mac 1 expression on eosinophils . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
However , the ability of the F 105 anti gp 120 monoclonal antibody ( specific for the CD 4 binding site ) to neutralize ICAM 1 bearing virions was diminished when target cells were pretreated with an lymphocyte function associated antigen 1 ( LFA 1 ) activating antibody . ^^^ Interestingly , ICAM 1 / POS progeny viruses were found to be slightly more resistant to neutralization by individual human sera in target cells expressing a low affinity form of LFA 1 than viruses devoid of host encoded ICAM 1 proteins . ^^^ These results suggest that the interaction between virally embedded host ICAM 1 and target cell surface LFA 1 should be considered a factor modulating neutralization sensitivity of HIV 1 by human sera from HIV 1 infected individuals . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
RESULTS : Human ICAM 1 was able to adhere to ligands on porcine cells , including one or more ligand that contains CD 18 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The hyperadhesivity of A / R exposed HUVEC was inhibited by monoclonal antibodies directed against either LFA 1 , VLA 4 , ICAM 1 , or VCAM 1 , indicating a contribution of these adhesion molecules and their ligands . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
By using a newly established CD 99 monoclonal antibody , MT99 / 3 , we show here that LFA 1 / ICAM 1 independent cell adhesion pathways are activated via CD 99 . ^^^ The Jurkat cell aggregation was not blocked by addition of CD11a ( LFA 1 ) and CD 54 ( ICAM 1 ) mAbs . ^^^ Furthermore , MT99 / 3 treatment did not alter the expression of LFA 1 and ICAM 1 molecules . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
At the site of contact between T cells and antigen presenting cells ( APCs ) , T cell receptor ( TCR ) peptide major histocompatibility complex ( MHC ) interaction is intensified by interactions between other molecules , notably by CD 28 and lymphocyte function associated antigen 1 ( LFA 1 ) on T cells interacting with B 7 ( B 7 1 and B 7 2 ) , and intracellular adhesion molecule 1 ( ICAM 1 ) , respectively , on APCs . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Cell arrest was dependent on application of shear and activation of Mac 1 and LFA 1 to bind ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
While addition of adhesion molecule blocking antibodies ( anti intercellular adhesion molecule 1 ( ICAM 1 ) , vascular cell adhesion molecule 1 ( VCAM 1 ) , CD 18 and very late antigen 4 ( VLA 4 ) ) strongly inhibited adherence of U 1 cells to endothelial monolayers , such treatment resulted in only a partial reduction in p 24 release . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The aim of our study was to assess the percentage of T lymphocytes expressing LFA 1 , Mac 1 and ICAM 1 in bronchoalveolar fluid ( BALF ) and blood of patients with sarcoidosis , atopic bronchial asthma and chronic bronchitis . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Both types of implants undergoing rejection expressed intercellular adhesion molecule 1 ( ICAM 1 ) and leukocyte function antigen 1 ( LFA 1 ) at high density ; BD and the underlying kidney parenchyma also expressed class 1 and 2 major histocompatibility complex ( MHC ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Minimal ICAM 1 and leukocyte function associated ( LFA 1 ) protein and mRNA expression is observed during early anagen and maximal expression during late anagen and catagen . ^^^ Keratinocytes of the distal outer root sheath , fibroblasts of the perifollicular connective tissue sheath , and perifollicular blood vessels exhibit maximal ICAM 1 immunoreactivity during catagen , which corresponds to changes of LFA 1 expression on perifollicular macrophages . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
METHOD OF STUDY : Pregnant CBA / J female mice mated with DBA / 2 male mice were injected with monoclonal antibodies ( MAbs ) to intercellular adhesion molecule 1 ( ICAM 1 ) and leukocyte function associate antigen 1 ( LFA 1 ) . ^^^ RESULTS : Significant suppression of fetal resorption was observed by the injection of MAb to ICAM 1 and LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
DESIGN : The expressions of very late activation antigen 4 ( VLA 4 ) , lymphocyte function associated antigen 1 ( LFA 1 ) , vascular cell adhesion molecule 1 ( VCAM 1 ) , and intercellular adhesion molecule 1 ( ICAM 1 ) were determined immunocytochemically , and levels of soluble VCAM 1 , ICAM 1 , and E selectin , by means of enzyme immunoassay technique . ^^^ In RRMS , LFA 1 and ICAM 1 expression in blood was more up regulated than in SPMS ( P = . 03 and P = . 01 , respectively ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Rats were injected with anti neutrophil serum or F ( ab ' ) 2 fragments of irrelevant mouse IgG2a ( control ) , anti ICAM 1 antibody , or anti CD 18 antibody . ^^^ By 6 hr , vascular congestion had disappeared , and ICAM 1 expression had markedly increased in venules in deep portions of the mucosa and submucosa , accompanied by an increase in the number of CD 18 positive neutrophils . ^^^ Neutrophil depletion , and immunoneutralizing of ICAM 1 and CD 18 inhibited the increase in MPO activity , and decreased ulcer sizes measured at 48 hr . ^^^ In conclusion , ET 1 causes ischemia reperfusion injury , and neutrophil accumulation after reperfusion mediated by the ICAM 1 CD 18 pathway may be important in the development of ET 1 induced gastric ulcer . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To support these functions , LFA 1 must convert from a resting to an activated state that avidly binds its ligands such as intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^ Substitution of residues in the core of this second 1 domain site resulted in constitutively active LFA 1 binding to ICAM 1 . ^^^ These results indicate that LFA 1 binding to ICAM 1 is regulated by an 1 domain allosteric site ( IDAS ) and that this site is structurally linked to the MIDAS . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Neutrophil surface expression of CD11b and CD 18 and endothelial cell intercellular adhesion molecule 1 ( ICAM 1 ) and vascular adhesion molecule 1 ( VCAM 1 ) were assessed by flow cytometry and an enzyme immunoassay , respectively . ^^^ RESULTS : The expression of CD11b and CD 18 on neutrophils and neutrophil dependent adhesion to endothelial cells elicited by HPE were inhibited by lansoprazole and omeprazole at clinical relevant doses , and the expression of ICAM 1 and VCAM 1 on endothelial cells and endothelial dependent neutrophil adherence induced by IL 1beta were also inhibited by lansoprazole and omeprazole at similar doses . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Expression of LFA 1alpha / CD11a , LFA 1beta / CD18 and ICAM 1 / CD56 were rapidly elevated and remained high whereas MAC 1 , CD 44 and VCAM 1 / CD106 were less widely expressed . ^^^ To identify the importance of VLA 4 , CD 44 , ICAM 1 and MAC 1 in the pathogenesis of SFV infection , monoclonal antibodies that block these adhesion molecules were administered in vivo during infection . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Intrinsic ICAM 1 / LFA 1 activation mediates altered responsiveness of atopic asthmatic airway smooth muscle . ^^^ To elucidate the mechanism of CAM related mediation of altered airway responsiveness in the atopic asthmatic state , the expressions and actions of intercellular adhesion molecule 1 ( ICAM 1 ) and its counterreceptor ligand lymphocyte function associated antigen 1 ( LFA 1 ; i . e . , CD11a / CD18 ) were examined in isolated rabbit airway smooth muscle ( ASM ) tissues and cultured human ASM cells passively sensitized with sera from atopic asthmatic patients or nonatopic nonasthmatic ( control ) subjects . ^^^ Collectively , these observations provide new evidence demonstrating that activation of the CAM counterreceptor ligands ICAM 1 and LFA 1 , both of which are endogenously expressed in ASM cells , elicits autologously upregulated IL 5 release and associated changes in ICAM 1 expression and agonist responsiveness in atopic asthmatic sensitized ASM . . ^^^ These proasthmatic changes in agonist responsiveness were ablated by pretreating the atopic sensitized tissues with a monoclonal blocking antibody ( MAb ) to either ICAM 1 or CD11a , whereas a MAb directed against the related beta ( 2 ) integrin Mac 1 had no effect . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Induction of unresponsiveness to islet xenograft by MMC treatment of graft and blockage of LFA 1 / ICAM 1 pathway . ^^^ Two different modalities [ direct graft treatment by mitomycin C ( MMC ) and blockage of the lymphocyte function associated antigen 1 / intracellular adhesion molecule 1 ( LFA 1 / ICAM 1 ) pathway in recipients by species specific mAbs ] were tested for their ability to produce unresponsiveness to secondary islet xenografts . ^^^ Recipient mice were divided into experimental groups according to anti rat ICAM 1 and / or anti mouse LFA 1 mAb treatment and transplantation of MMC treated or nontreated islets . ^^^ One of six , four of nine , and six of eight animals accepted MMC treated islet xenografts over 100 days after treatment with anti rat ICAM 1 , anti mouse LFA 1 , or both mAbs treatments , respectively , whereas none of the animals accepted nontreated islets under the same treatment . ^^^ When the mice bearing long term functioning xenografts were challenged with the secondary graft from the original donor strain , the animals previously treated with anti recipient LFA 1 and anti donor ICAM 1 mAbs were more prone to accept it than animals given anti recipient LFA 1 mAb alone ( MST : 55 . 8+ / 25 . 7 vs . 15+ / 2 . 4 respectively ; P < 0 . 001 ) , although they rejected the third party xenograft and allograft acutely . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The role of LFA 1 , Mac 1 , ICAM 1 and Ia in the induction of Th 2 type of immune response in spleen during murine syngeneic pregnancy . ^^^ Immunochemical localization of ICAM 1 , LFA 1 , Mac 1 and Ia in spleen have been carried out at different stages of pregnancy and formation of cell clusters and natural cell adhesion assay with splenocytes were carried out on day 1 ( D 1 ) pregnancy and compared with control . ^^^ Upregulation of ICAM 1 , LFA 1 , Mac 1 and Ia was observed during early pregnancy . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
One of them , cytohesin 1 , a 47 kDa cytoplasmic protein acts as an inside out signaling molecule and regulates binding of the beta 2 integrin leukocyte function antigen 1 ( LFA 1 ) to its ligand intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To this effect , we have analysed the expression of LFA 1 ; VLA 4 , ICAM 1 ; CD44H and CD44v6 ( haematopoietic and variant form respectively ) on freshly isolated lymphocytes obtained from bone marrow ( BM ) , peripheral blood ( PB ) and lymph node ( LN ) by flow cytometry . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Preincubation of cells with CD11b or CD 18 antibody specifically decreased adhesion to either BSA or ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In conclusion , this unique study has demonstrated for the first time the important role of the active form of LFA 1 on the lymphocyte cell surface for conjugate formation with an ICAM 1 expressing tumor cell ; also , two pathways of cell signaling were identified for conjugate formation to occur . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Activation of Stat 3 leads to homotypic cell aggregation , increased expression of intercellular adhesion molecule 1 ( ICAM 1 ) , CD 18 , and CD11b , and activation of signaling through CD 18 containing integrins . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The LFA 1 integrin supports rolling adhesions on ICAM 1 under physiological shear flow in a permissive cellular environment . ^^^ The LFA 1 integrin is crucial for the firm adhesion of circulating leukocytes to ICAM 1 expressing endothelial cells . ^^^ In the present study , we demonstrate that LFA 1 can arrest unstimulated PBL subsets and lymphoblastoid Jurkat cells on immobilized ICAM 1 under subphysiological shear flow and mediate firm adhesion to ICAM 1 after short static contact . ^^^ However , LFA 1 expressed in K 562 cells failed to support firm adhesion to ICAM 1 but instead mediated K 562 cell rolling on the endothelial ligand under physiological shear stress . ^^^ LFA 1 mediated rolling required an intact LFA 1 1 domain , was enhanced by Mg2+ , and was sharply dependent on ICAM 1 density . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Cleaved high molecular weight kininogen binds directly to the integrin CD11b / CD18 ( Mac 1 ) and blocks adhesion to fibrinogen and ICAM 1 . ^^^ The functional significance of direct interaction of HKa to Mac 1 was investigated by examining the effect of HKa on cellular adhesion to fibrinogen and intercellular adhesion molecule 1 ( ICAM 1 ) , molecules abundant in the injured vessel wall . ^^^ HKa blocked the adhesion of Mac 1 transfected cells to fibrinogen and ICAM 1 in a dose dependent manner . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
However , the single and double mutants R52E and T91Q on the CFG face of domain D 1 , which correspond to the key residues Glu ( 34 ) and Gln ( 73 ) for ICAM 1 binding to LFA 1 , had no effect on LFA 1 binding . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In this study , the early inflammatory response and time related expression of intercellular adhesion molecule 1 ( ICAM 1 ) and vascular cell adhesion molecule 1 ( VCAM 1 ) and their ligands , leukocyte function antigen 1 ( LFA 1 ) and very late antigen 4 ( VLA 4 ) , in the grafts were investigated in RCMV infected rats and compared to noninfected rats developing chronic rejection . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To isolate LFA 1 / ICAM 1 interactions from Mac 1 / ICAM 1 interactions , full length chimeric ICAM 1 was developed and expressed in L cells with domains 1 and 2 from canine ICAM 1 and domains 3 5 from human ICAM 1 ( C 1 , 2 ; H 3 5 ) . ^^^ We demonstrated that chimeric ICAM 1 C 1 , 2 ; H 3 5 supported only LFA 1 dependent adhesion of canine neutrophils and that such adhesion triggered rapid onset of H2O2 production from canine neutrophils . ^^^ The following seven experimental conditions distinguished LFA 1 dependent H2O2 production from Mac 1 dependent production : It did not require exogenous chemotactic stimulation ; H2O2 release was more rapid , but the amount released was < 40 % of that mediated by Mac 1 adhesion ; it was inhibited by anti CD11a and anti ICAM 1 antibodies ; in contrast to that mediated by Mac 1 , it was not inhibited by anti CD11b antibody , neutrophil inhibitory factor ( NIF ) , or cytochalasin B or H 7 . ^^^ Thus , canine neutrophils seem to be able to utilize two members of the beta 2 integrin family to interact with ICAM 1 and signal H2O2 production , with LFA 1 at an early stage without prior chemotactic stimulation and Mac 1 at a later stage requiring chemotactic stimulation . . ^^^ The respiratory burst of neutrophils stimulated by chemotactic factors is markedly augmented by Mac 1 dependent adhesion such as the interaction of Mac 1 ( CD11b / CD18 ) with intercellular adhesion molecule 1 ( ICAM 1 ; CD 54 ) expressed on the surface of parenchymal cells ( e . g . , cardiac myocytes ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
T595B1 expressed CD 3 , T cell receptor ( TCR ) beta , TCR Vbeta 2 , CD 4 , CD 25 , CD45RB , CD 44 , LFA 1 , and ICAM 1 molecules on its cell surface and showed MHC class 2 1 E ( k ) restricted tumor antigen specific proliferation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Expression of intercellular adhesion molecule 1 ( ICAM 1 ) and lymphocyte function associated antigen 1 ( LFA 1 ) on TIL cells were analyzed on FACScan following incubation with FITC conjugated antibody . ^^^ The interaction of TIL with FN increased expression of ICAM 1 and LFA 1 on TIL cells as well . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Semiquantitative immunohistochemistry was performed for CD8+ T cells , ICAM 1 , VCAM 1 , LFA 1 , and class 1 HLA in frozen sections of myocardial biopsies from patients presenting chronic chagasic cardiomyopathy ( group 1 , n = 12 ) , heart allograft rejection ( group 2 , n = 9 ) or dilated cardiomyopathy ( group 3 , n = 9 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Expression of LFA 1 ( CD11a / CD18 ) and ICAM 1 ( CD 54 ) in an animal model of renal interstitial fibrosis induced by unilateral ureteral obstruction . ^^^ In this study , the development of fibrosis in the UUO model of the rat was examined and its relation to the time course of LFA 1 and ICAM 1 expression was assessed by immunohistochemistry . ^^^ This study shows that LFA 1 expression and ICAM 1 expression are concordant and that this process is associated with increasing interstitial fibrosis . ^^^ ICAM 1 interstitial tissue may facilitate the homing and persistence of an interstitial infiltrate by ICAM 1 / LFA 1 interactions , thereby preceding the development of renal interstitial fibrosis . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To investigate the effect of LFA 1 and CD 44 on the adhesion of lymphocytes to the pulmonary endothelium , lymphocytes preincubated with an anti LFA 1 MoAb , which blocks the interaction of LFA 1 and intercellular adhesion molecule 1 ( ICAM 1 ) , or lymphocytes preincubated with an anti CD 44 MoAb , were injected . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
METHODS AND RESULTS : PECAM 1 , CD 34 , ICAM 1 , VCAM 1 , VLA 4 , P and E selectin , the macrophage antigens Ki M1P and Mac 1 , and lymphocyte function antigen LFA 1 were identified immunohistochemically . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Serial colonic sections ( 10 UC , 10 CD , and 10 controls ) were stained immunohistochemically for ICAM 1 , ICAM 2 , ICAM 3 , CD11a , CD11b , CD 18 , and PECAM 1 . ^^^ In conclusion , CD11b , CD 18 , and ICAM 2 seem to be important for PMN transepithelial migration in UC , whereas CD11a , CD11c , ICAM 1 , and ICAM 3 seem central in leukocyte locomotion and aggregation in CD . ^^^ Comparative studies of the colonic in situ expression of intercellular adhesion molecules ( ICAM 1 , 2 , and 3 ) , beta 2 integrins ( LFA 1 , Mac 1 , and p 150 , 95 ) , and PECAM 1 in ulcerative colitis and Crohn ' s disease . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
On monocytes , LPS and MDP increased surface expression of human leukocyte antigen DR ( HLA DR ) , CD 18 , CD 54 ( intercellular adhesion molecule 1 , ICAM 1 ) , and CD 86 ( B 7 2 ) . ^^^ The early increase ( 1 6 h ) of CD 18 and ICAM 1 expression led us to hypothesize that CD 18 dependent costimulatory signals were involved in the later ( 6 h ) increase of monocyte HLA DR expression . ^^^ However , blocking studies using monoclonal antibodies against CD 18 ( IB 4 , 15 microg / ml ) demonstrated that the LPS and MDP induced increase of HLA DR and ICAM 1 expression on monocytes was not mediated through CD 18 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The intercellular adhesion molecule 1 ( ICAM 1 , CD 54 ) serves as a counter receptor for the beta 2 integrins , LFA 1 and Mac 1 , which are expressed on leukocytes . ^^^ This adhesion was inhibited by antibodies specific for either ICAM 1 or LFA 1 / Mac 1 , suggesting a role for these molecules in adhesion . ^^^ Nonetheless , within each cell line , blocking of ICAM 1 or LFA 1 / Mac 1 reduced LAK cell killing , suggesting that ICAM 1 is involved in mediating this killing . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The surface expressed LFA 1 variants are constitutively active with respect to ICAM 1 adhesion and these variants express the activation reporter epitope of the mAb 24 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Strong expression of LFA 1 and ICAM 1 was observed in the skin after the TL transplantation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Adherence of adoptively transferred alloreactive Th 1 cells in lung : partial dependence on LFA 1 and ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In this sequential study , the expression of ICAM 1 and VCAM 1 and their ligands LFA 1 and VLA 4 as well as major histocompatibility complex class 2 antigens ( MHC class 2 ) , and interleukin 2 receptor ( IL 2R ) were investigated during the development of chronic renal allograft rejection in a rat model . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To elucidate the key molecules involved in bone resorption and cell infiltration associated with cholesteatoma , we examined the in vivo levels of IL 1 alpha and TNF alpha , intercellular adhesion molecule 1 ( ICAM 1 ) and lymphocyte functional antigen 1 ( LFA 1 ) in acquired and congenital cholesteatomas , by reverse transcriptase polymerase chain reaction , immunohistochemistry , and ELISA . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The mRNA expression of tumor necrosis factor alpha ( TNFalpha ) , interleukin 1beta ( IL 1beta ) , inducible and endothelial nitric oxide synthase ( iNOS and eNOS ) , endothelin 1 ( ET 1 ) , E selectin ( CD62E ) , intercellular adhesion molecule 1 ( ICAM 1 ) and its ligand CD 18 , and CD 25 was evaluated with semiquantitative reverse transcription polymerase chain reaction ( RT PCR ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The more potent compounds were highly selective and did not affect U 937 cell adhesion to fibronectin ( VLA 5 ) , phorbolmyristate acetate or PMA differentiated U 937 cell adhesion to intercellular cell adhesion molecule 1 ( ICAM 1 ) expressing Chinese hamster ovary cells ( LFA 1 ) and adenosine diphosphate ( ADP ) induced platelet aggregation ( GPIIb / IIIa ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Antibodies to both ICAM 1 and LFA 1 do not protect the kidney against toxic ( HgCl 2 ) injury . ^^^ METHODS : Female Wistar rats were injected with monoclonal antibodies ( mAbs ) directed to both the leukocyte function associated antigen 1 ( LFA 1 ) and the intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^ RESULTS : Injected antibodies were demonstrated on peripheral blood leukocytes ( flow cytometrical detection of mouse anti LFA 1 ) and on endothelium ( immunohistochemical staining of mouse anti ICAM 1 ) and were measured in serum ( enzyme linked immunosorbent assay ) . ^^^ Kidneys from ischemia exposed rats were , however , functionally protected by identical anti ICAM 1 / anti LFA 1 treatment . ^^^ CONCLUSION : Prevention of cellular infiltration by mAbs to LFA 1 and ICAM 1 has no effect on renal morphology , function , or regeneration following mercuric chloride induced ARF in the rat . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The bile ducts express intercellular adhesion molecule 1 ( ICAM 1 ) and vascular cellular adhesion molecule 1 ( VCAM 1 ) , and infiltrating lymphocytes express LFA 1 and VLA 4 , facilitating their interaction . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Further , the changes of these markers and costimulatory molecules [ LFA 1 ( CD11a ) , ICAM 1 ( CD 54 ) , CD 40 and CD 40 ligand ( CD 154 ) ] were also examined in the responsive patients . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Immunohistochemical studies on liver sections from CCl ( 4 ) treated animals indicated , that in the areas of necrosis 24 48 h after a single administration of the toxin , there was an accumulation of LFA 1 , ED 1 ( marker for rat monocytes ) and ICAM 1 positive , but ED 2 ( marker for tissue macrophages ) negative inflammatory cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The second domain of intercellular adhesion molecule 1 ( ICAM 1 ) maintains the structural integrity of the leucocyte function associated antigen 1 ( LFA 1 ) ligand binding site in the first domain . ^^^ The first domain of intercellular adhesion molecule 1 ( ICAM 1 ) binds to the leucocyte function associated antigen 1 ( LFA 1 ) 1 domain , which contains the principal ligand binding site of this leucocyte integrin . ^^^ LFA 1 on T cells also binds with reduced affinity to this ICAM 1 mutant . ^^^ In summary our data suggest that the second domain of ICAM 1 has a role in maintaining the structure of the LFA 1 ligand binding site in the first domain of ICAM 1 but does not appear to have a direct role in ligand binding . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
OBJECTIVE : This study examined the effects of acute psychological stress and exhaustive exercise on the expression and density of adhesion molecules ( L selectin , lymphocyte function antigen 1 [ LFA 1 ] , and intracellular adhesion molecule 1 [ ICAM 1 ] ) on monocytes , granulocytes , and lymphocytes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
CD 18 and ICAM 1 dependent corneal neovascularization and inflammation after limbal injury . ^^^ Using a relevant mouse model of limbal injury , we examined the role of CD 18 and intercellular adhesion molecule 1 ( ICAM 1 ) in limbal injury induced neovascularization . ^^^ The results show that CD 18 and ICAM 1 deficient mice developed 35 % ( n = 5 , P = 0 . 003 ) and 36 % ( n = 5 , P = 0 . 002 ) less neovascularization than strain specific normal controls , respectively . ^^^ Taken together , these data identify CD 18 and ICAM 1 as mediators of the inflammatory and VEGF dependent corneal neovascularization that follows limbal injury . ^^^ The targeting of CD 18 and ICAM 1 may prove useful in the treatment of inflammation associated neovascularization in the cornea and elsewhere . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We thereby propose that the higher affinity adhesion via LFA 1 / ICAM 1 is prerequisite for efficient function of membrane bound ODF during osteoclast maturation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The leukocyte specific beta 2 integrin LFA 1 ( alphaLbeta 2 ) is the most important integrin expressed by leukocytes that regulate lymphocyte migration and the initiation of an immune response through binding to ICAM 1 , 2 or 3 . ^^^ Although alterations in the affinity of LFA 1 , which leads to enhanced ICAM 1 binding , have been proposed , evidence is emerging that dynamic reorganisation of LFA 1 into microclusters is the major mechanism that regulates its binding capacity . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Renal function and morphology were determined ; frozen sections were immunostained for P selectin , L selectin , intercellular adhesion molecule 1 ( ICAM 1 ) , vascular cell adhesion molecule 1 ( VCAM 1 ) , platelet endothelial cell adhesion molecule 1 ( PECAM 1 ) , leukocyte function associated molecule 1 ( LFA 1 ) , very late antigen 4 ( VLA 4 ) , as well as for neutrophils and monocytes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We determined the extent to which these molecules contribute to PMN infiltration by utilizing mice deficient ( / ) in P selectin , E selectin , ICAM 1 , and CD 18 . ^^^ Deficiencies of P selectin , E selectin , ICAM 1 , or CD 18 resulted in significant ( P < 0 . 05 ) attenuation of PMN infiltration into the I / R myocardium ( MI / R ) . ^^^ In addition , P selectin , E selectin , ICAM 1 , and CD 18 / mice exhibited significantly ( P < 0 . 05 ) smaller areas of necrosis after MI / R compared with wild type mice . ^^^ Furthermore , genetic deficiency of P selectin , E selectin , ICAM 1 , or CD 18 attenuates PMN sequestration and myocardial injury after in vivo MI / R . ^^^ We conclude that P selectin , E selectin , ICAM 1 , and CD 18 are involved in the pathogenesis of MI / R injury in mice . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The interaction between leukocyte function associated antigen 1 ( LFA 1 ) , a member of the beta ( 2 ) integrin family of adhesion molecules , and intracellular adhesion molecule ICAM 1 ( cd 54 ) is thought to play a critical role in the inflammatory process . ^^^ In conjunction with fine tuning of the diaryl substituents , we discovered a novel series of potent , nonpeptide inhibitors of LFA 1 / ICAM 1 interaction , exemplified by A 286982 ( 28h ) , which has IC ( 50 ) values of 44 and 35 nM in an LFA 1 / ICAM 1 binding assay and LFA 1 mediated cellular adhesion assay , respectively . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Adhesion of the monocytic cell line THP 1 and of peripheral monocytes and granulocytes to immobilized ICAM 1 was impaired by unfractionated heparin , to the same extent as with inhibition of Mac 1 by monoclonal antibodies such as c7E3 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Mac 1 expression , as well as ICAM 1 expression , was higher in control SHRSP than in WKY . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The leukocyte integrins LFA 1 and Mac 1 bind to endothelial intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
RESULTS : In cholesterol treated rats , endotoxin significantly increased glomerular numbers of PMN and MO , glomerular expression of ICAM 1 and VCAM 1 and glomerular numbers of LFA 1 and VLA 4 positive cells as compared with saline . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Linear and cyclic LFA 1 and ICAM 1 peptides inhibit T cell adhesion and function . ^^^ Here , several 10 amino acid peptides derived from the contact domains of LFA 1 and ICAM 1 were evaluated for their ability to interfere with intercellular adhesion by T cells and to inhibit a more biologic , mixed lymphocyte reaction . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Extended studies demonstrated that : 1 ) ASM cells express mRNAs and proteins for the cell adhesion molecules ( CAMs ) / costimulatory molecules , CD 40 , CD40L , CD 80 , CD 86 , ICAM 1 ( CD 54 ) , and LFA 1 ( CD11a / CD18 ) ; 2 ) apart from LFA 1 , ASM cell surface expression of the latter molecules is up regulated in the presence of activated T cells ; and 3 ) pretreatment of ASM cells and tissues with mAbs directed either against CD11a or the combination of CD 40 and CD 86 completely abrogated both the activated T cell induced changes in expression of the above CAMs / costimulatory molecules in ASM cells and altered ASM tissue responsiveness . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Vehicle cells that expressed the ICAM 1 ligands , CD11b and CD 18 , were obtained from bone marrow cells of DBA / 2j mice and adenovirally transduced with the IL 1ra gene or glucocerebrosidase ( GC ) gene ex vivo . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
LFA 1 and its ligands , ICAM 1 , ICAM 2 , and ICAM 3 , can be expressed on the cells infected by HIV , as well as on the HIV virions themselves . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Expression of adhesion molecules LFA 1 ( CD11a and ICAM 1 ( CD 54 ) on lymphocytes and chemokines IL 8 and MCP 1 concentrations in bronchoalveolar lavage of patients with asthma or chronic obstructive pulmonary disease ] . ^^^ The aim of this study was to determine the numbers and percentages of lymphocytes expressing adhesion molecules : LFA 1 , ICAM 1 together with assessment of chemokines concentrations : IL 8 and MCP 1 in bronchoalveolar lavage fluid ( BAL ) of patients with asthma or chronic obstructive pulmonary disease ( COPD ) . 12 patients with asthma , 14 patients with COPD , and 6 subjects of control group took part in this study . ^^^ The expression of LFA 1 and ICAM 1 was assessed on lymphocytes by using immunohistochemistry ( streptavidyn biotin , DAKO , Denmark ) . ^^^ The percentage of lymphocytes expressing LFA 1 and ICAM 1 were : 33 . 9 + / 23 . 8 % and 25 . 8 + / 12 . 2 % in COPD patients , 23 . 9 + / 12 . 1 % and 15 . 3 + / 4 . 42 % in asthma patients , and 14 . 2 + / 10 % and 5 . 2 + / 1 . 6 % in the control group respectively . ^^^ There was observed significant difference between the percentage of lymphocytes expressing LFA 1 and ICAM 1 of COPD and the control group . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In addition to the TCR ligand interaction , other receptor ligand pairs , such as LFA 1 and ICAM 1 , play a major role in the activation of T cells . ^^^ Recent studies of T cell activation suggest a coordinated movement of LFA 1 and ICAM 1 in forming a defined zone in the immunological synapse . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
LFA 1 , CD45RO , chemokine receptors , focal adhesion kinase ) , to a central polarizing compartment ( MTOC , PKC , MARCKS ) , or into the uropod ( CD 44 , CD 43 , ICAM 1 and 3 , beta 1 integrins ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
At both ages , similar mRNA levels were seen in the hippocampus across genotypes for ICAM 1 , A 20 , and MAC 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In this report , we studied monocyte / macrophage infiltration and expression of intercellular adhesion molecule 1 ( ICAM 1 ) and macrophage antigen 1 ( Mac 1 ) in weanling rats with UUO . ^^^ Monocyte / macrophage infiltration and expression of ICAM 1 and Mac 1 were evaluated immunohistochemically , and results were compared with those of sham operated control rats ( SOK ) . ^^^ CONCLUSIONS : Adhesion molecules , ICAM 1 and Mac 1 , are expected to recruit monocyte / macrophage infiltration into OBK of weanling rats with UUO . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Structurally , exosomes were found to harbor immunologically relevant molecules such as MHC class 2 , CD 86 , LFA 1 , and ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Due to the elongated , twig resembling shapes and numerous negatively charged rests of sialic acid CD 43 can act in an anti adhesive fashion e . g . , disturbing ICAM 1 ( CD 54 ) LFA 1 ( CD11A / CD18 ) interaction . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Expression of intercellular adhesion molecule 1 ( ICAM 1 ) and its ligand , leukocyte function antigen 1 ( LFA 1 ) , after pancreatic islet transplantation may affect both nonspecific and alloantigen specific phases of graft destruction . ^^^ We examined the effects of ICAM 1 / LFA 1 blockade on the survival of islet allografts . ^^^ However , dual ICAM 1 / LFA 1 blockade with either ICAM 1 oligo / alphaLFA 1 mAb ( 78 . 3 + / 16 . 5 days ) or ( alphaICAM 1 mAb / aLFA 1 mAb ( 65 . 2 + / 31 . 3 days ) was the most effective therapy . ^^^ Furthermore , ICAM 1 / LFA 1 blockade improved islet function as evaluated by glucose tolerance test , and decreased inflammation in comparison with untreated controls . ^^^ Thus , ICAM 1 / LFA 1 blockade prolongs the survival of pancreatic islet allografts and improves their early function . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Infiltrating host cells and their products were assessed in chronically rejecting grafts by histology and immunostaining using mAbs for monocyte / macrophages , T cells , ICAM 1 , LFA 1 and cytokines . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Specific pretreatment with monoclonal antibodies against ICAM 1 , LFA 1 , or other soluble mediators may be useful in down regulating hyperacute rejection in trans species transplantation . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
A panel of monoclonal antibodies specific for inflammatory cells ( T lymphocytes , polymorphonuclear leukocytes , macrophages , dendritic cells ) and different adhesion molecules ( E selectin , ICAM 1 , VCAM 1 , LFA 1 , VLA 4 ) was used . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Adhesion molecules such as ICAM 1 , LFA 1 and VLA are not involved . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Binding of a novel CD 18 activation epitope mAb to LFA 1 in response to soluble ICAM 1 binding was also blocked by inhibitory and was enhanced by activating IDAS mutations . ^^^ Binding of the LFA 1 ligand , ICAM 1 to the metal ion dependent adhesion site is regulated by the 1 domain allosteric site ( IDAS ) . ^^^ We demonstrate here that intracellular signaling leading to activation of LFA 1 binding to ICAM 1 is regulated at the IDAS . ^^^ In addition , mutational activation at the IDAS greatly increases the binding of lymphocyte expressed LFA 1 to ICAM 1 in response to PMA , but does not result in constitutive binding . ^^^ Surface plasmon resonance using soluble wild type LFA 1 and an IDAS mutant of LFA 1 indicate that the IDAS can regulate a 6 fold change in the K ( d ) of ICAM 1 binding . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Monocytic cell adhesion to intact and plasmin modified fibrinogen : possible involvement of Mac 1 ( CD11b / CD18 ) and ICAM 1 ( CD 54 ) . beta ( 2 ) integrin Mac 1 and immunoglobulin like ICAM 1 adhesion molecules are expressed by monocytes and both known to bind fibrinogen and its degradation products . ^^^ Using several cell types , characterized by different patterns of Mac 1 and ICAM 1 expression , and monoclonal antibodies against beta ( 2 ) integrins and ICAM 1 we demonstrate , that fibrinogen cleavage evokes gradual decrease in beta ( 2 ) integrin dependent cell adhesion . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Since these mAbs have distinct properties in terms of epitope expression and effect on LFA 1 binding to ICAM 1 , we conclude that the beta ( 2 ) NTR and mid region interact extensively . ^^^ Finally , the mAbs H 52 and 7E4 , abrogated KIM 185 but not Mg / EGTAinduced LFA 1 / ICAM 1 binding and the epitope of MEM 148 is expressed on Mg / EGTA activated but not resting LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In this work , the changes in expression of the adhesion molecules ICAM 1 / LFA 1 on inflammatory cells of the liver were studied by immunohistochemistry . ^^^ A significant upregulation of ICAM 1 and LFA 1 in both the SEA group and the infected control group started shortly after egg deposition at day 35 and persisted up to day 56 p . i . ^^^ Notably , both ICAM 1 and LFA 1 expression peaks were shifted earlier to day 38 p . i . in the SEA group compared to day 40 in the infected control group . ^^^ The distribution of ICAM 1 and LFA 1 in both groups was comparable . ^^^ At the early phase of infection before granuloma formation , both ICAM 1 and LFA 1 were detected along the sinusoidal wall of small blood vessels . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Cholesterol depletion made cells resistant to SDF 1 induced binding to ICAM 1 through LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We found that antibodies against VCAM 1 ( CD 106 ) , VLA 4 ( CD49d / CD29 ) , ICAM 1 ( CD 54 ) , and LFA 1 ( CD11a / CD18 ) did not influence the increased number of antigen specific T cells in the CSF However , upon intravenous ( i . v . ) injection , anti PECAM 1 ( CD 31 ) antibody or PECAM Ig chimeric molecule inhibited the trafficking of alpha / beta TCR+ Vbeta3+ Mac 1 cells into the CNS . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Inhibition of Rho ( either by treatment with C 3 exoenzyme or transfection with a dominant negative form of Rho ( N19Rho ) ) or ROCK ( by treatment with Y 27632 ) results in the following : ( a ) partial disorganization and aggregation of cortical filamentous actin ( F actin ) ; ( b ) induction of LFA 1 mediated cellular adhesion to the LFA 1 ligand intercellular adhesion molecule 1 ( ICAM 1 ) through a mechanism involving clustering of LFA 1 molecules , rather than alterations in the level of expression or in the affinity state of this integrin ; and ( c ) induction of cellular polarization and activation of the tyrosine kinase PYK 2 . ^^^ Importantly , the activation of PYK 2 caused by inhibition of Rho or ROCK takes place only when the T cells are plated onto ICAM 1 but not when they are either prevented from interacting with ICAM 1 with anti LFA 1 blocking antibodies or when they are plated on the nonspecific poly l lysine substrate . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
LFA 1 is normally expressed in a quiescent state on the surface of leukocytes and interacts weakly with its ligands ICAM 1 , 2 , and 3 . ^^^ We have recently described a small molecule antagonist of LFA 1 , BIRT 377 , that demonstrates selective in vitro and in vivo inhibition of LFA 1 / ICAM 1 mediated binding events . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
TNFalpha in both a dose and time dependent fashion induced expression of CD11a ( LFA 1 ) , CD 54 ( intercellular adhesion molecule 1 , ICAM 1 ) , CD 106 ( vascular cell adhesion molecule 1 , VCAM 1 ) , CD49d ( very late activating antigen 4 , VLA 4 ) , and / or MUC 1 on MM cell lines ; as well as CD 106 ( VCAM 1 ) and CD 54 ( ICAM 1 ) expression on BMSCs . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We determined the time course of these responses to Gram negative sepsis in the mouse lung and addressed the specific contributions of CD 18 integrins and ICAM 1 . ^^^ Saturating concentrations of anti CD 18 and anti ICAM 1 mAbs reduced lung tissue PMN sequestration and migration ; however , peak responses at 3 and 6 h were inhibited by 40 % , indicating that only a small component of PMN sequestration and migration was CD 18 dependent at these times . ^^^ In contrast to the time dependent decreased role of CD 18 integrins in mediating PMN sequestration and migration , CD 18 and ICAM 1 blockade prevented the increase in lung microvascular permeability and edema formation at all times after E . coli challenge . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Blood samples taken 24 h preoperatively from 49 patients ( 3 18 years old ) were stained with monoclonal antibodies for adhesion molecules ( ICAM 1 , LFA 1 , Mac 1 ) or constitutive / activation markers ( CD 4 , CD 14 , CD 16 , CD 25 , CD 54 , CD 69 , HLA DR ) and measured on a microbead calibrated FCM . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
IL 18 time and concentration dependently upregulated the expression of intercellular adhesion molecule 1 ( ICAM 1 ) in a monocyte population in human PBMC as determined by FACS analysis while the expression of CD11a , CD 18 , CD 29 , CD 44 , and CD62L in monocytes and that of ICAM 1 , CD11a , CD 18 , CD 29 , CD 44 , and CD62L in T cells was not influenced by IL 18 . ^^^ IL 18 also induced the aggregation of PBMC , which was prevented by anti ICAM 1 and anti LFA 1 Abs . ^^^ On the other hand , anti ICAM 1 and anti LFA 1 Abs inhibited IL 18 induced production of three cytokines , IL 12 , IFN gamma , and TNF alpha , by 60 and 40 % , respectively . ^^^ These results strongly suggested that the IL 18 induced upregulation of ICAM 1 and the subsequent adhesive interaction through ICAM 1 on monocytes and LFA 1 on T / NK cells generate an additional stimulatory signaling as well as an efficient paracrine environment for the IL 18 initiated cytokine cascade . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The interaction of LFA 1 and ICAM 1 plays an important role in the cell adhesion process . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
This was due to lymphocyte function associated antigen ( LFA ) 1 intercellular adhesion molecule ( ICAM ) 1 interaction , because 2D6 ( IL 12 ) expressed both LFA 1 and ICAM 1 and cell aggregation was inhibited by anti ICAM 1 monoclonal antibody . ^^^ Despite comparable levels of LFA 1 and ICAM 1 expression , 2D6 ( IL 2 ) cells did not aggregate in culture with IL 2 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Leukocyte adherence mediated by intercellular adhesion molecule 1 ( ICAM 1 ) binding to leukocyte function associated antigen ( LFA 1 ) is required for proper inflammatory and immune function . ^^^ Inhibition of ICAM 1 \ LFA 1 binding using monoclonal antibodies ( mAb ) has been shown to be efficacious at inhibiting lymphoma metastasis as well as leukocyte emigration into tissue in a number of inflammatory diseases such as ischemia reperfusion injury , septic shock and rheumatoid arthritis . ^^^ Thus , we identified a unique molecule that inhibits ICAM 1 dependent cell adhesion , but is not related to the primary sequence of the ICAM 1 ligand LFA 1 . ^^^ Due to the small size and ability to block cell cell adhesion , IP 01 may serve as a useful tool for study of ICAM 1 and LFA 1 biology as well as for the development of small molecule therapeutics . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
These results suggest that ESM 1 and ICAM 1 interact with LFA 1 on binding sites very close to but distinct from the 1 domain of CD11a . ^^^ Through this mechanism , ESM 1 could be implicated in the regulation of the LFA 1 / ICAM 1 pathway and may therefore influence both the recruitment of circulating lymphocytes to inflammatory sites and LFA 1 dependent leukocyte adhesion and activation . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
SDF 1alpha triggered a transient regulation of adhesion to intercellular adhesion molecule 1 ( ICAM 1 ) and vascular cell adhesion molecule 1 mediated by lymphocyte function antigen 1 ( LFA 1 ) and very late antigen 4 ( VLA 4 ) , respectively , and a rapid increase in LFA 1 binding to soluble ICAM 1 . ^^^ Inhibition of PI 3 K blocked the up regulation of Jurkat cell adhesion to ICAM 1 by SDF 1alpha , whereas inhibition of mitogen activated protein kinase kinase impaired the subsequent down regulation and blocking both pathways abrogated LFA 1 regulation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Inhibition of leukocyte sticking and infiltration , but not rolling , by antibodies to ICAM 1 and LFA 1 in murine endotoxin induced uveitis . ^^^ Each mouse concurrently received an intraperitoneal injection of monoclonal neutralizing antibodies for ICAM 1 , LFA 1 , or both or control irrelevant antibodies . ^^^ Mice that received anti ICAM 1 or anti LFA 1 had significantly fewer cells infiltrating their irises at 6 and 24 hours . ^^^ Detailed analysis of the 6 hour time point recordings revealed that neither anti ICAM 1 nor anti LFA 1 significantly reduced the number of leukocytes rolling on venule endothelial surfaces , but the treatments reduced the number of firmly adherent cells . ^^^ CONCLUSIONS : These data confirm previous reports that ICAM 1 and LFA 1 are important mediators of EIU . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Expressions of L selectin ( 93 . 0 + / 5 . 7 % , p < 0 . 05 vs arterial ) , CD11a ( 98 . 8 + / 3 . 8 % , p=0 . 06 ) , CD 18 ( 96 . 9 + / 4 . 0 % , p < 0 . 05 vs arterial ) , and ICAM 1 ( 89 . 1 + / 7 . 7 % , p < 0 . 05 ) on polymorphonuclear neutrophils ( PMN ) , and arteriovenous leukocyte counts ( arterial : 103 . 5 + / 5 . 4 % , venous : 91 . 1 + / 3 . 3 % , p < 0 . 05 ) decreased . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The cecal peritoneum 24 hr after perforation was stained with specific antibodies against LFA 1 , Mac 1 , VLA 4 , ICAM 1 , VCAM 1 , and fibronectin diluted with cold University of Wisconsin ( UW ) solution in conjunction with immuno gold labeling . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
METHODS : The expression of ICAM 1 and its natural ligand , lymphocyte function associated antigen 1 ( LFA 1 ) , in normal nasal mucosa from 6 controls and in nasal polyp tissue from 19 patients with nasal polyposis were determined with immunohistochemistry . ^^^ RESULTS : The expression of ICAM 1 and LFA 1 was stronger in the nasal polyp tissue than in normal nasal mucosa . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Combination of anti ICAM 1 and anti LFA 1 monoclonal antibody therapy prolongs allograft survival in rat hind limb transplants . ^^^ In this study , the authors investigated the effects of anti ICAM 1 and anti LFA 1 therapy in the rat hind limb cremaster transplantation model . ^^^ Group 1 animals received only vehicle solution ; Groups 2 and 3 received monoclonal antibodies against ICAM 1 and LFA 1 , respectively ; Group 4 received a combination dose . ^^^ The activation of adhering leukocytes was significantly lower in rats treated with anti ICAM 1 , anti LFA 1 , and combination than in controls ( p < 0 . 05 ) . ^^^ The first clinical signs of rejection were noted between the 5 ( th ) and 9 ( th ) days in the control group , on the 9 ( th ) day in the anti ICAM 1 or anti LFA 1 groups , and on the 13 ( th ) day with combination therapy . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
A fourfold higher density of ICAM 1 on beads markedly increased the rate of binding to LFA 1 but did not change the early and narrow time window for the optimal rate of adhesion . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Therefore , we examined the impact of LFA 1 : ICAM 1 interactions on transcriptional and posttranscriptional IL 2 gene regulation , using a costimulation negative cell line transfected with MHC class 2 alone , or in combination with ICAM 1 or B 7 1 . ^^^ These results indicate that the primary pathway whereby engagement of LFA 1 through its ligand ICAM 1 up regulates IL 2 gene expression is through enhanced IL 2 transcription , in the absence of any effect on IL 2 mRNA stabilization . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In a high cholesterol / cholate model , a role of ICAM 1 and CD 18 integrins was also shown , but this awaits confirmation in more physiologic models . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Lack of age associated LFA 1 up regulation and impaired ICAM 1 binding in lymphocytes from patients with Down syndrome . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
LFA 1 and ICAM 1 antibody idarubicin conjugates separately prolong murine cardiac allograft survival . ^^^ It has been reported that , in mice , antibodies to ICAM 1 and LFA 1 have to be used together to induce tolerance to cardiac allografts ; here we show that these monoclonal antibodies , conjugated to idarubicin , can lead to tolerance induction to cardiac allografts when used alone . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In a purified system , HK and HKa inhibited the binding of soluble fibrinogen and ICAM 1 to immobilized Mac 1 , but not the binding of ICAM 1 to immobilized LFA 1 ( CD11a / CD18 , alphaLbeta 2 ) . ^^^ Accordingly , HK , HKa , and domain 5 inhibited the adhesion of Mac 1 but not LFA 1 transfected K 562 human erythroleukemic cells to ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Agglutination induced by A 80 was inhibited by stromal cell derived factor 1 , T 22 , and phorbol 12 myristate 13 acetate but was not significantly altered by pretreatment of cells with pertussis toxin , wortmannin , or MAbs to LFA 1 , ICAM 1 , ICAM 2 , and ICAM 3 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Because surface molecules are involved in the regulation of migration and proliferation , their expression ( lymphocyte function associated antigen 1 [ LFA 1 ] , intercellular adhesion molecule 1 ( ICAM 1 ) , L selectin , alpha 4 integrins , CD 44 , major histocompatability complex class 2 , interleukin 2 receptor alpha chain ) was determined on B and T cell subsets of both groups . ^^^ B cells , but not T cells , showed a significantly reduced LFA 1 and ICAM 1 expression in blood and lymph nodes , whereas the expression of the other surface molecules analyzed remained unchanged . ^^^ Thus , the presence of one organ is necessary for appropriate expression of LFA 1 and ICAM 1 on B cells in other , distant organs . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We also find that these neoepitopes are present on rLFA 1 with high affinity for ICAM 1 and their binding is modulated in parallel with the affinity of LFA 1 for ICAM 1 . ^^^ Collectively , these neoepitope Abs identify a subpopulation of LFA 1 most likely with high affinity for ICAM 1 and necessary for LFA 1 function . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We studied this regulation by selecting a Jurkat T cell clone , J lo1 . 3 , that expresses LFA 1 yet fails to bind to purified ICAM 1 despite treatment of the cells with PMA or Mn2+ . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The expression of ICAM 1 protein and the number of Mac 1 positive cells were measured by immunohistochemical staining . ^^^ RESULTS : The expression of ICAM 1 protein and the number of Mac 1 positive cells were reduced markedly in the obstructed kidneys of the mice treated with the ICAM 1 ASON ( 110 + / 14 [ symbol : see text ] / mm2 vs 270 + / 13 [ symbol : see text ] / mm2 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We have shown that p arylthio cinnamides can inhibit the interaction of LFA 1 and ICAM 1 , which is involved in cell adhesion and the inflammatory process . ^^^ Compounds 39 and 40 which contain two CF 3 groups have IC ( 50 ) values of 0 . 5 and 0 . 1 nM , respectively , in inhibiting JY 8 cells expressing LFA 1 on their surface , from adhering to ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Subsequent challenge with LPS i . p . was associated with upregulation of Mac 1 and ICAM 1 on PMN , but not of VLA 4 or LFA 1 ( FACS analysis ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
OBJECTIVE : To study the distribution of cytoskeletal proteins ( actin , alpha actinin , vinculin , beta tubulin , keratin , vimentin , desmin ) , adhesion molecules for cell matrix interations ( very later antigens [ VLA 1 61 , beta 1 , beta 2 [ CD 18 ] , vitronectin receptor [ alphavbeta 3 ] , CD 11b ) , leukocyte adhesion molecules ( ICAM 1 ) and extracellular matrix proteins ( collagen 4 , fibronectin , laminin , vitronectin ) in human and rat kidneys by using a superior processing and immunohistochemical staining technique . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
By using HUT 78 T cell lines , CD 4 ligands were shown to induce a dissociation of LFA 1 from cytohesin , a cytoplasmic protein known to bind LFA 1 and to enhance the affinity / avidity of LFA 1 for its ligand ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
OBJECTIVE : To study the role of intercellular adhesion molecule 1 ( ICAM 1 ) and lymphocyte function associated antigen 1 ( LFA 1 ) in the pathogenesis of viral hepatitis B . ^^^ METHODS : ICAM 1 , LFA 1 and CD ( 8 ) expression in the livers of 11 healthy persons and 70 patients with HBV infection were studied by using immunohistochemical techniques . ^^^ Hepatic coexpression of ICAM 1 and LFA 1 were studied using double staining immunohistochemical techniques . ^^^ RESULTS : In the areas of inflammation and necrosis , intensive infiltration of LFA 1 positive lymphocytes were often observed around hepatocytes with enhanced ICAM 1 expression , with some of these lymphocytes in close contact with hepatocytes expressing ICAM 1 . ^^^ CONCLUSION : The interaction of ICAM 1 and LFA 1 is involved in mediating the adhesion of lymphocytes and hepatocytes , and it plays an important part in the immunopathogenesis of chronic viral hepatitis B and severe viral hepatitis B . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha ( TNF alpha ) , which is produced by some MM cells , induces only low level MM proliferation and MAPK activation in MM cells , but markedly upregulates IL 6 secretion from BMSCs and upregulates expression of adhesion molecules ( VLA 4 and LFA 1 ) on MM cells and their receptors ( VCAM 1 and ICAM 1 ) on BMSCs , with resultant increased binding of MM cells to BMSCs . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
RESULTS : 1 ) Thrombin activated platelets adhered to resting HUVEC at dynamic flow conditions through platelet glycoprotein IIb / IIIa and RGD proteins . 2 ) Neutrophils tethered to P selectin induced on thrombin activated platelets , which were immobilized on HUVEC . 3 ) Activated neutrophils adhered , via LFA 1 , to ICAM 1 on HUVEC . 4 ) Activated platelets induced interleukin ( IL ) 8 secretion by HUVEC . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Whereas the alphaL / beta2RB7 LFA 1 variant is wildtype like with respect to ICAM 1 adhesion , the alphaLbeta2BN1 and alphaLbeta2BN7 , as well as the alphaLbeta2RN7 , variants are more adhesive than the wildtype . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Conversion of LFA 1 to a high affinity high avidity state for ICAM 1 further augmented the susceptibility of human tonsillar histocultures to infection by ICAM 1 bearing virions . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Synergistic inhibitory activity of alpha and beta LFA 1 peptides on LFA 1 / ICAM 1 interaction . ^^^ Interactions of cell adhesion molecule LFA 1 and its ligand ICAM 1 play important roles during immune and inflammatory responses . ^^^ Critical residues of LFA 1 for ICAM 1 binding are known to be in the 1 domain of the alpha subunit and the 1 like domain of the beta subunit . ^^^ L on LFA 1 / ICAM 1 interaction , here we have explored the activity of 1 like domain peptide LBE on the binding mechanism of cLAB . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Generation of an LFA 1 antagonist by the transfer of the ICAM 1 immunoregulatory epitope to a small molecule . ^^^ The protein protein interaction between leukocyte functional antigen 1 ( LFA 1 ) and intercellular adhesion molecule 1 ( ICAM 1 ) is critical to lymphocyte and immune system function . ^^^ Here , we report on the transfer of the contiguous , nonlinear epitope of ICAM 1 , responsible for its association with LFA 1 , to a small molecule framework . ^^^ These LFA 1 antagonists bound LFA 1 , blocked binding of ICAM 1 , and inhibited a mixed lymphocyte reaction ( MLR ) with potency significantly greater than that of cyclosporine A . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Role of LFA 1 / ICAM 1 , CLA / E selectin and VLA 4 / VCAM 1 pathways in recruiting leukocytes to the various regions of the chronic leg ulcer . ^^^ The multiple region biopsy approach , which enables to study the different development phases of the ulcer at one time point , was employed to immunohistochemically identify the vascular adhesion molecules E selectin , intercellular adhesion molecule 1 ( ICAM 1 ) and vascular cell adhesion molecule 1 ( VCAM 1 ) , and their counter ligands on extravasated leukocyte cutaneous lymphocyte associated antigen ( CLA ) , lymphocyte function associated antigen ( LFA 1 ) and very late activation antigen 4 ( VLA 4 ) , respectively . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Quantitative expression of the adhesion receptors VLA 4 , VLA 5 , L selectin , MAC 1 , and ICAM 1 on the surface of CD34+ cells ] . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule 1 ( ICAM 1 ) was up regulated by p 43 and mediated p 43 induced cell cell adhesion via the interaction with LFA 1 or Mac 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule 1 ( ICAM 1 ) and the leukocyte integrin CD 18 are required for these processes . ^^^ Following one week of diabetes , neutrophil CD11a , CD11b , and CD 18 expression was increased significantly , as were retinal ICAM 1 levels . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In the present study , the role of LFA 1 / ICAM 1 ligation in human Th cell polarization was investigated . ^^^ Triggering of LFA 1 on anti CD3 / CD28 stimulated naive Th cells with immobilized Fc ICAM 1 , in the absence of DC and exogenous cytokines , induced a marked shift toward Th 1 cell development , accompanied by a dose dependent decrease in GATA 3 expression and a dose dependent increase in T bet expression . ^^^ Blocking the ICAM 1 / LFA 1 interaction in cocultures of these DC with naive Th cells attenuated their Th 1 driving capacity . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
LFA 1 / ICAM 1 and alpha ( 4 ) integrins / VCAM 1 supported rolling , but were not relevant to rolling velocity . ^^^ Firm arrest was mainly mediated by LFA 1 and ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Synthetic peptides derived from the P ( 0 ) protein and leukocyte function associated antigen 1 ( LFA 1 ) were investigated as potential ligands for targeting liposomes to intercellular adhesion molecule 1 ( ICAM 1 ) expressing melanoma cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Down regulation of cell adhesion molecules LFA 1 and ICAM 1 after in vitro treatment with the anti TNF alpha agent thalidomide . ^^^ The present study investigates down regulation of cell adhesion molecules ( ICAM 1 and LFA 1 ) and decreases in cell cell contacts between human T leukemic ( CEM ) cells and human umbilical vein endothelial cells ( HUVEC ) after thalidomide exposure . ^^^ CEM cells were cultured in RPMI 1640 medium with 0 , 10 or 50 microg / ml thalidomide , stained with fluorescent monoclonal antibodies specific to ICAM 1 and LFA 1 and expression was measured with flow cytometry . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Previous work has shown that intercellular adhesion molecule 1 ( ICAM 1 ) and CD 18 are required for these processes . ^^^ However the relevant in vivo stimuli for ICAM 1 and CD 18 expression in diabetes remain unknown . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Enhanced T lineage acute lymphoblastic leukaemia cell survival on bone marrow stroma requires involvement of LFA 1 and ICAM 1 . ^^^ Lympho cyte function associated antigen 1 ( LFA 1 ) on T ALL cell lines bound intercellular adhesion molecule 1 ( ICAM 1 ) on HS 5 monolayers , and survival was inhibited 85 98 % with monoclonal antibodies directed against LFA 1 or ICAM 1 . ^^^ We compared these results with patient derived T ALL lymphoblasts co cultured on either HS 5 BM or normal BM monolayers and found that LFA 1 and ICAM 1 were required , but not alone sufficient for ex vivo leukaemic cell survival . ^^^ This is the first report to demonstrate the importance of LFA 1 / ICAM 1 mediated adhesion as a critical event in a cascade of cell surface receptor ligand interactions that regulate T ALL survival in the BM microenvironment . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Adhesion molecules were of great importance in contra suppression , as antibody treatment to LFA 1 or ICAM 1 blocked this activity . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Inhibition of LFA 1 / ICAM 1 and VLA 4 / VCAM 1 as a therapeutic approach to inflammation and autoimmune diseases . ^^^ LFA 1 is exclusively expressed on leukocytes and interacts with its ligands ICAM 1 , 2 , and 3 to promote a variety of homotypic and heterotypic cell adhesion events required for normal and pathologic functions of the immune systems . ^^^ Linear and cyclic peptides derived from the sequences of LFA 1 , ICAM 1 , ICAM 2 , VCAM 1 , and FN C 1 have been shown to have inhibitory effects in vitro and in vivo . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Furthermore , lymphocytes preincubated with monoclonal antibodies to lymphocyte function associated antigen 1 ( LFA 1 ) or HB MVEC preincubated with monoclonal antibodies to intercellular adhesion molecule 1 ( ICAM 1 ) significantly down regulated the adhesion probability between lymphocytes and endothelial cells , indicating that the adhesion probability is related to the expression of LFA 1 on lymphocytes and to the expression of ICAM 1 on endothelial cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Herein , we show that Ca ( 2 ) ( + ) mobilizing agents A 23187 , thapsigargin , and ionomycin induce an increase in adhesion to the LFA 1 ligand intercellular adhesion molecule 1 ( ICAM 1 ) and activation and redistribution of the proline rich tyrosine kinase 2 ( PYK 2 ) to the microtubule organizing center ( MTOC ) in T lymphoblasts . ^^^ Most importantly , Ca ( 2 ) ( + ) mobilization did not induce activation of PYK 2 when the LFA 1 / ICAM 1 interaction was prevented with function blocking mAb , implying that the Ca ( 2 ) ( + ) induced activation of PYK 2 requires integrin engagement . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
METHODS : Rats were assigned to one of six groups including sham operation , intestinal IR ( 60 / 120 min ) and IR plus treatment with one of the following monoclonal antibodies against CD11a , CD11b , CD 18 , and ICAM 1 . ^^^ The increase in pulmonary microvascular permability and neutrophil accumulation elicited by intestinal reperfusion was effectively prevented by administration of blocking antibodies against ICAM 1 , CD 11 , and CD 18 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
METHODS : By using immunohistochemical techniques , the expression of CD ( 4 ) ( + ) cells , CD ( 8 ) ( + ) cells , macrophages , IL 2 receptor , MHC 2 antigen , intercellular adhesion molecule 1 ( ICAM 1 ) and lymphocyte function associated antigen 1 ( LFA 1 ) in rejected corneal grafts were studied . ^^^ RESULTS : Immunohistochemical studies showed diffuse infiltration of CD ( 4 ) ( + ) cells , CD ( 8 ) ( + ) cells and macrophages , and strong expression of IL 2 receptor , MHC 2 antigen , ICAM 1 and LFA 1 in rejected corneal grafts , especially in the acute rejection stage . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The role of Mac 1 and ICAM 1 molecules in adhesion of cells on fibronogen and its degradation products ] . ^^^ Monocytic cell adhesion to immobilized fibrinogen and fibrinogen degradation products , and involvement of integrins Mac 1 and immunoglobulin like ICAM 1 adhesion molecules in these processes were investigated . ^^^ Fibrinogen cleavage with plasmin down regulated adhesion of cells with predominant Mac 1 expression ; in contrast , the attachment of ICAM 1 expressing was up regulated . ^^^ By means of function blocking anti Mac 1 and anti ICAM 1 antibodies , and immobilization of known fibrinogen degradation products , it was shown that Mac 1 molecules mediated cell adhesion predominantly to fibrinogen , and its early degradation products , fragments 10 and Y , while ICAM 1 participated in cell attachment to 10 and Y fragments , rather than to intact fibrinogen or late degradation products , fragments D and E . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Essential role of ICAM 1 / LFA 1 interaction in synergistic effect of IL 18 and IL 12 on IFN gamma production in human PBMC . ^^^ In the present study , we examined whether the synergistic upregulation of ICAM 1 occurred after the stimulation with the combination of IL 18 and IL 12 and whether the synergistic production of IFN gamma was dependent on the interaction between ICAM 1 on monocytes and LFA 1 on NK / T cells . ^^^ Anti ICAM 1 and anti LFA 1 Abs exhibited significant inhibitory effect on enhanced production of IFN gamma by the combination of two cytokines , in particular , anti ICAM 1 showing the complete inhibition . ^^^ These results as a whole indicated that synergistic effect of IL 18 and IL 12 on IFN gamma production in human PBMC is ascribed to the synergism of the effect of two cytokines on ICAM 1 expression on monocytes and that the subsequent ICAM 1 / LFA 1 interaction plays an important role in the enhanced production of IFN gamma . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Tethering of neutrophils to brain endothelium is mediated by selectins , and subsequent adhesion and migration by endothelial intercellular adhesion molecule 1 ( ICAM 1 ) and neutrophil CD 18 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In wild type mice , antibodies against CD 18 , ICAM 1 ( an endothelial ligand for CD 18 ) , or VCAM 1 given 30 minutes before ischemia did not block neutrophil emigration at 3 hours reperfusion . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Expression of adhesion molecules LFA 1 and ICAM 1 on osteoclast precursors during osteoclast differentiation and involvement of estrogen deficiency . ^^^ The adhesion molecules , leukocyte function associated antigen 1 ( LFA 1 ) and intercellular adhesion molecule 1 ( ICAM 1 ) , are necessary for osteoclast formation . ^^^ In this study , the expression of LFA 1 and ICAM 1 on osteoclast precursors during osteoclast differentiation , and the involvement of ovariectomy in the expression , were investigated . ^^^ On days 3 , 5 and 7 of culture , the expression of LFA 1 and ICAM 1 on osteoclast precursors was quantitated using indirect immunofluorescence and confocal laser cytometry , and , on day 7 , the number of formed osteoclasts was measured by tartrate resistant acid phosphatase ( TRAP ) stain . ^^^ RESULTS : The level of ICAM 1 expression on osteoclast precursors gradually increased with osteoclast differentiation , whereas that of LFA 1 did not change . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The expression of lymphocyte function associated antigen 1 ( LFA 1 ) and its ligand , intercellular adhesion molecule 1 ( ICAM 1 ) , was most frequent on ATLL cells infiltrating the stomach and was enhanced substantially on vascular endothelium in H . pylori infected gastric mucosa . ^^^ CONCLUSIONS : ATLL cells infiltrate gastric tissues infected with H . pylori , probably through the interaction of adhesion molecules on these cells and their ligands on the vasculature , i . e . , through the LFA 1 / ICAM 1 pathway . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In this work we compared the expression of VLA 4 , VLA 5 , Mac 1 , ICAM 1 and VCAM 1 adhesion molecules , at ultrastructural level , between blood monocytes and alveolar macrophages obtained from BAL , from patients with pulmonary sarcoidosis . ^^^ Cells obtained from blood and BAL were fixed , embedded in LRWhite and then ultrathin sections were incubated with monoclonal antibodies against VLA 4 , VLA 5 , Mac 1 , ICAM 1 and VCAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
MATERIAL / METHODS : 140 EMBs from dilated cardiomyopathy ( DCM ) patients and 14 autopsy heart samples ( controls ) were immunostained for T lymphocytes ( CD 2 , CD 3 , CD 4 , CD 8 ) , beta ( 2 ) integrin+ infiltrates ( CD 18 , LFA 1 , Mac 1 ) and CAMs ( immunoglobulin superfamily : ICAM 1 , HLA class 1 , HLA DR , VCAM 1 , CD 58 ; selectins : CD62E and CD62P ; and the beta ( 1 ) integrin chain CD 29 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Structural and ICAM 1 docking properties of a cyclic peptide from the 1 domain of LFA 1 : an inhibitor of ICAM 1 / LFA 1 mediated T cell adhesion . ^^^ We found that cyclic peptide 1 can bind to the D 1 domain of ICAM 1 and inhibit ICAM 1 / LFA 1 mediated homotypic and heterotypic T cell adhesion . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
A conformational switch in the 1 domain allosteric site ( IDAS ) and in CD 18 regulates LFA 1 affinity for endothelial ligands including intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^ We examined the dynamics of CD 18 activation in terms of the efficiency of neutrophil capture of ICAM 1 , and we correlated this with the membrane topography of 327C , an antibody that recognizes the active conformation of CD 18 1 like domain . ^^^ A threshold dose of approximately 75 pm IL 8 , corresponding to ligation of only approximately 10 100 receptors , was sufficient to activate approximately 20 , 000 CD 18 and a rapid boost in the capture efficiency on ICAM 1 . ^^^ Shear resistant arrest on a monolayer of ICAM 1 was reversed within minutes of chemotactic stimulation correlating with a shift from high to low affinity CD 18 and dispersal of patches of active CD 18 . ^^^ Dynamic regulation of LFA 1 activation and neutrophil arrest on intercellular adhesion molecule 1 ( ICAM 1 ) in shear flow . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
METHODS : Frozen sections of 9 normal nasal mucosa and 19 nasal polyp samples were studied with immunohistochemical technique and immunohistochemical and MGG dual staining method with ICAM 1 and LFA 1 monoclone antibodies . ^^^ RESULTS : Expression of ICAM 1 and LFA 1 were stronger in nasal polyps tissue than in the normal nasal mucosa , and there is positive relationship between the expression of ICAM 1 and the expression of LFA 1 on eosinophils . ^^^ CONCLUSION : High expression of ICAM 1 and LFA 1 in nasal polyps tissue showed that interaction of ICAM 1 / LFA 1 may participate in the inflammatory reaction and prompt adhesion and migration of eosinophils . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Monocyte and neutrophil expression of adhesion molecules Mac 1 , LFA 1 and 3 , ICAM 1 , and VLA 4 were measured at 0 , 120 , and 240 minutes by using laser flow cytometry . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The immunological synapse formed during mature T cell activation consists of a central cluster of TCR and MHC molecules surrounded by a ring of LFA 1 and ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
A second signal also can be delivered through LFA 1 residing on the T cell , responding to its counter receptor ICAM 1 residing on a different cell . ^^^ Finally , the combination of anti CD 3 plus anti ICAM 1 ( but not anti CD 3 alone ) caused prolonged proliferation of naive T cells in a manner similar to costimulation through LFA 1 or CD 28 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Using flow cytometry , 98 % of the B16F1 melanoma cells expressed VLA 4 , the counter receptor of VCAM 1 , and approximately 1 . 5 % expressed LFA 1 , the counter receptor of ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
This binding is dependent on the applied stress and is independent of Galalpha 1 , 3 gal structures , ICAM 1 , or its counter ligands LFA 1alpha and Mac 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
This study analyzes the consequences of signaling events initiated by the interaction of the leukocyte integrin LFA 1 with its ligand , dimeric ICAM 1 . ^^^ We show that the active form of LFA 1 regulates its own function on primary human T cells by directing the remodeling of the F actin cytoskeleton to strengthen T cell adhesion to ICAM 1 . ^^^ The study went on to show that clustering of conformationally altered LFA 1 is essential for the changes in F actin , and a model is proposed in which clustered , high avidity T cell LFA 1 , interacting with multivalent ICAM 1 , causes LFA 1 signaling , which results in F actin polymerization and higher order F actin bundling . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The expression of adhesion molecules ( CD11a , CD11b , CD 18 , CD49d , CD 54 , CD62L ) on peripheral blood mononuclear cells ( PBMCs ) of asthmatics and the expression of E and P selectins , ICAM 1 , VCAM 1 on cultured human umbilical vein endothelial cells ( HUVEC ) after stimulation with PBMCs from CDAs before and after 3 and 6 months of SeS were assessed by standard monoclonal antibodies and analyzed by flow cytometry . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Pretreatment with blocking antibodies to intercellular adhesion molecule 1 ( ICAM 1 ; CD 54 ) , a ligand of LFA 1 and Mac 1 , did not result in an effect on G CSF induced mobilization , suggesting that the enhancing effect required an interaction of the beta 2 integrins and one of their other ligands . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
These results suggest that LSP 1 regulates the function of Mac 1 ( CD11b / CD18 ) , which binds only to fibrinogen and ICAM 1 among the substrates we tested . fMLP induced filamentous actin polarization is also increased in the absence of LSP 1 when cells were layered on fibrinogen , but not on fibronectin . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Interactions between leukocyte function associated antigen 1 ( LFA 1 ) and intercellular adhesion molecule 1 ( ICAM 1 ) influence the development of osteoclasts . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
All the cytokines tested ( interleukin 1beta ( IL 1beta ) , IL 2 , IL 4 , IL 7 , IL 10 , interferon gamma ( IFN gamma ) ) as well as CAMs ( ICAM 1 , ICAM 2 , VCAM 1 , LFA 1 and CD 44 ) were expressed at substantial levels in both spleen and LNs . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Neither TAT dnRas nor PD 98059 blocked eosinophil adhesion to ICAM 1 , up regulation of CBRM1 / 5 , or focal surface clustering of Mac 1 caused by PMA . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Here we show that MZ B cells express elevated levels of the integrins LFA 1 ( alphaLbeta 2 ) and alpha4beta1 and that they bind to the ligands ICAM 1 and VCAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The aim of this study was to evaluate the association of the expression of the adhesion molecules CD11a , CD11b , CD 18 , CD 54 , CD49d , and CD 62 L in white blood cells and levels of soluble adhesion molecules ICAM 1 and L selectin in serum with occupational exposure to styrene . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Strikingly , stimulation of LFA 1 avidity to intercellular adhesion molecule 1 ( ICAM 1 ) by the same chemokines , although G ( 1 ) dependent , was insensitive to raft disruption . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
METHODS : The 18 OLP and 10 normal oral mucosa biopsies were immunostained for CD 4 , CD 8 , CD1a , LFA 1 , VCAM 1 and ICAM 1 . ^^^ Those cells through ICAM 1 and LFA 1 promote epithelial destruction . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
After I / R , lack of enteral feeding increases organ expression of ICAM 1 , CD11b levels on myeloid cells , and lung of CD 18 positive leukocytes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Histomorphological evaluation of the vessels was performed , and endothelial expression of ICAM 1 , VCAM 1 , and E selectin was also evaluated , as well as distribution of LFA 1 , Mac 1 , VLA 4 , RM3 / 1 , 27E10 , and 25F9 bearing cells in the subepithelial tissue . ^^^ RESULTS : The expression of ICAM 1 was downregulated after radiotherapy , whereas the percentage of LFA 1 and VLA 4 bearing cells increased . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The results have been compared to those obtained after target cells treatment with monoclonal antibodies recognizing adhesion molecules ICAM 1 ( CD 54 ) and LFA 3 ( CD 58 ) ( which are able to block some of the receptors implicated in conjugation ) , as well as with cholera toxin ( CTX ) that can modify the state of affinity of some adhesion molecules such as LFA 1 ( CD11a / CD18 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Administration of monoclonal antibodies directed against the leukocyte function associated antigen 1 ( LFA 1 ) intercellular adhesion molecule 1 ( ICAM 1 ) pathway showed that these costimulatory molecules play a key role in allograft rejection . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The cell aggregation of U 937 cells was completely blocked , even in the presence of the 70 kDa fragment , by mAb against leukocyte function associated antigen 1 ( LFA 1 ) or intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^ We therefore propose that TSP 1 may regulate LFA 1 / ICAM 1 mediated cell adhesion of monocytes / macrophages by either the inhibitory effect through CD 47 or the promoting effect through CD 36 depending on which domain / fragment is functional in a given biological setting . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Briefly , we discuss three means by which LFA 1 is adapted to bind more efficiently to its chief ligand , ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In this report the authors confirm this hypothesis with mAbs directed against lymphocyte function associated antigen 1 ( [ LFA 1 ] CD11a / CD18 ) , the molecule on the surface of leukocytes that interacts with ICAM 1 . ^^^ Twenty nine animals were then randomized into three groups and received intraperitoneal injections of anti LFA 1 mAb ( 10 rats ) , anti ICAM 1 mAb ( 10 rats ) , or an isotype matched control mAb ( nine rats ) . ^^^ Results of FACS analysis demonstrated saturation of both LFA 1 and ICAM 1 binding sites in animals treated with the respective mAb . ^^^ The mean ratios of blood exposed to saline exposed luminal cross sectional areas ( expressed as the percentage of lumen patency ) were 90 . 1 + / 5 . 8 % ( mean + / standard error of the mean ) for animals treated with the anti LFA 1 mAb ( p = 0 . 0218 ) , 94 . 2 + / 3 . 3 % for animals treated with the anti ICAM 1 mAb ( p = 0 . 0067 ) , and 62 + / 7 . 4 % for animals treated with the isotype matched control mAb . ^^^ Macrophage and granulocyte counts in the periadventitial region were 39 . 5 + / 3 . 2 / hpf for animals treated with anti LFA 1 mAb ( p = 0 . 001 ) , 42 + / 3 . 7 / hpf for animals treated with anti ICAM 1 mAb ( p = 0 . 003 ) , and 72 . 2 + / 6 . 2 / hpf for control animals . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Interactions between leukocyte function associated antigen 1 ( LFA 1 ) with its cognate ligand , intercellular adhesion molecule 1 ( ICAM 1 ) play a crucial role in leukocyte adhesion . ^^^ Because the cell and its adhesive components are subject to external perturbation from the surrounding flow of blood , it is important to understand the binding properties of the LFA 1 / ICAM 1 interaction in both steady state and in the presence of an external pulling force . ^^^ Here we report on atomic force microscopy ( AFM ) measurements of the unbinding of LFA 1 from ICAM 1 . ^^^ The single molecule measurements revealed the energy landscape corresponding to the dissociation of the LFA 1 / ICAM 1 complex and provided the basis for defining the energetic determinants of the complex at equilibrium and under the influence of an external force . ^^^ The AFM force measurements were performed in an experimental system consisting of an LFA 1 expressing T cell hybridoma , 3A9 , attached to the end of the AFM cantilever and an apposing surface expressing ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
A recruitment of B 220 ( + ) and MAC 1 ( + ) cells with an up regulated expression of MHC class 1 , CD 80 ( B7 . 1 ) and CD 54 ( ICAM 1 ) was observed in nasal associated lymphoid tissues from MALP 2 treated mice . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The kinetics of G CSF stimulated adhesion to ICAM 1 peaked at 11 min without detectable surface upregulation of Mac 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Because B ALL cells can evade T cell / natural killer cell recognition by down regulation of cell surface accessory molecules that participate in the formation of a functional immunologic synapse , we compared the CD 19 specific effector function of genetically modified CD 8 ( + ) CTLs toward CD 19 ( + ) cells with disparate levels of intercellular adhesion molecule 1 ( ICAM 1 ) , leukocyte function associated antigen 1 ( LFA 1 ) , and LFA 3 . ^^^ We observed that recognition of B lineage tumor lines by CD 19 specific CTLs was not impaired by low levels of ICAM 1 , LFA 1 , and LFA 3 cell surface expression , a functional attribute that is likely a consequence of our high affinity CD 19 specific chimeric immunoreceptor . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The expression of the following cell adhesion molecules , their beta 1 and beta 2 integrin ligands and the cytokine tumour necrosis factor alpha ( TNF alpha ) was investigated by light and electron microscope immunohistochemistry in the liver tissue in 20 patients with colorectal and gastric cancer also presenting with liver metastases : intercellular adhesion molecule 1 ( ICAM 1 ) , vascular endothelial adhesion molecule 1 ( VCAM 1 ) , E selectin , leucocyte function associated antigen 1 ( LFA 1 ) , macrophage antigen 1 ( Mac 1 ) , and very late antigen 4 ( VLA 4 ) . ^^^ Tumour infiltrating host cells sowing positive immunoreactivity for ICAM 1 , VCAM 1 , LFA 1 , Mac 1 , and VLA 4 were located mainly at the boundary between liver parenchyma and the metastasis . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Mycobacterium tuberculosis infected human macrophages exhibit enhanced cellular adhesion with increased expression of LFA 1 and ICAM 1 and reduced expression and / or function of complement receptors , FcgammaRII and the mannose receptor . ^^^ Quantitative transmission electron microscopy studies revealed that macrophage adhesion was accompanied by increases in levels of LFA 1 and its counter receptor ( ICAM 1 ) , decreases in surface levels of the phagocytic receptors CR 3 , CR 4 and FcgammaRII , and an increase in major histocompatibility complex Class 2 ( MHC 2 ) molecules at 72 h post infection . ^^^ The modulation of macrophage adhesion and phagocytic proteins may influence the trafficking of Mtb infected macrophages within the host , with increases in levels of LFA 1 and ICAM 1 enhancing the adhesive properties of the macrophage and decreases in phagocytic receptors diminishing the phagocytic capacity of an already infected cell , potentially allowing for maintenance of the intracellular niche of Mtb . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In addition , rolling velocities of lymphocytes from CD 18 hypomorphic mice were 48 % faster on 926 FtVII / ICAM 1 cells , with a similar reduction in rolling frequency relative to wild type lymphocytes . ^^^ Thus , in addition to mediating leukocyte firm adhesion , CD 18 integrin / ICAM 1 interactions regulate leukocyte rolling velocities and thereby optimize L selectin mediated leukocyte rolling . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Secondly , statins can inhibit LFA 1 adhesion to ICAM 1 and thus regulate T cell activation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To gain a profound insight into the underlying mechanisms , we phenotypically characterized pan leukocytes ( CD 18 ) , naive T lymphocytes ( CD 3 , CD 2 , CD 4 , CD 8 ) , activated lymphocytes ( LFA 1 , LFA 3 , VLA 4 , ICAM 1 , CD 69 , CD45R0 ) , macrophages ( Mac 1 , 27E10 ) , B lymphocytes ( CD 19 ) and NK cells ( CD 57 ) in DCM and control hearts . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Antibodies against LFA 1 and ICAM 1 as well as CD 44 markedly blocked the TEM of Th 1 cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Adhesion molecules ( ICAM 1 , VCAM 1 , ELAM 1 ) and their ligands ( LFA 1 , VLA 4 , sLeX ) and lymphoid activation markers ( MHC class 2 , IL 2R ) were demonstrated in liver biopsies by immunohistochemistry . ^^^ HHV 6 significantly ( P < or = 0 . 05 ) increased the vascular expression of ICAM 1 and VCAM 1 , and the number of graft infiltrating lymphocytes positive for LFA 1 , VLA 4 and class 2 antigens . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
IL 1beta is a co stimulator in activating Th cells , which manifests its activity together with LFA 1 , ICAM 1 and ICAM 2 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In resorption assays measuring either 45Ca release from bone organ cultures or pit formation in bone cell cultures , 1 , 25D , PTH and IL 1alpha stimulated resorption was inhibited by anti ICAM 1 monoclonal antibody ( mAb ) and / or anti LFA 1 mAb , while basal and PGE 2 stimulated bone resorbing activities were not affected by these mAbs . ^^^ Furthermore , in a mouse bone marrow culture system , stimulation of osteoclast like ( OCL ) cell formation by 1 , 25D ( 10 nM ) , PTH ( 10 ng / ml ) or IL 1alpha ( 10 ng / ml ) was inhibited by the addition of anti ICAM 1 mAb and / or anti LFA 1 mAb . ^^^ In a coculture system of murine spleen cells and osteoblasts , the ICAM 1 / LFA 1 interaction was also involved in 1 , 25D , PTH and IL 1alpha stimulated TRAP positive MNC formation . ^^^ However , anti ICAM 1 mAb and anti LFA 1 mAb did not alter either 1 , 25D or PTH stimulated receptor activator of NF kappaB ligand ( RANKL ) mRNA transcription in bone marrow cultures . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
METHOD : Samples of turbinate mucosa were from 20 cases of chronic hypertrophic rhinitis , 24 cases of non provocation period allergic rhinitis and 20 cases of provocation period allergic rhinitis and their paraffin sections were studied with immunohistochemical technique staining with ICAM 1 and LFA 1 monoclone antibodies . ^^^ RESULT : Expression of ICAM 1 and LFA 1 were higher in turbinate mucosa of provocation period allergic rhinitis than in chronic hypertrophic rhinitis and non provocation period allergic rhinitis turbinate mucosa ( P < 0 . 01 ) . ^^^ CONCLUSION : High expression of ICAM 1 and LFA 1 in turbinate mucosa of allergic rhinitis shows that the interaction of ICAM 1 / LFA 1 may participate in the inflammatory reaction of allergic rhinitis and may play an important role in the pathogenesis and development of allergic rhinitis . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecules ( ICAM 1 , VCAM 1 , and LFA 1 ) in adenocarcinoma of lung . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
It suppressed the accumulation of ICAM 1 positive and Mac 1 positive cells in the lamina propria ( LP ) and the expression of ICAM 1 and VCAM 1 by vascular endothelial cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Conversely , Jurkat cell LFA 1 H AFN adhesion to ICAM 1 was not associated with uPAR / LFA 1 complexes , any requirement for GPI anchored glycoproteins , or inhibition by beta glucan . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
RESULTS : Specific cation dependent binding of lymphocytes to bronchial endothelium was observed which was significantly inhibited by antibodies against P selectin , PSGL 1 , L selectin , LFA 1 , ICAM 1 and ICAM 2 but not E selectin , VLA 4 , VCAM 1 or Mac 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
HDACI induced homotypic aggregation was blocked by exposure to a monoclonal antibody specific for the beta chain ( CD 18 ) of an ICAM 1 ligand , LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Expression of the adhesion molecules , ICAM 1 , VCAM 1 , NCAM , CD 44 , CD49d ( VLA 4 , alpha chain ) , and CD11a ( LFA 1 , alpha chain ) on mouse oocytes , and pre and peri implantation stage embryos was examined by quantitative indirect immunofluorescence microscopy . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In splenectomized rats , the surface expression of LFA 1 and ICAM 1 is selectively reduced on B cell subsets in blood and lymph node , which is accompanied by a selective increase in the number of all B cell subsets in the blood . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
LFA 1 and intercellular adhesion molecule 1 ( ICAM 1 ) expression were analyzed by flow cytometry . ^^^ RESULTS : 5T33MMvt cells had a heterogeneous LFA 1 expression and all cells were positive for the LFA 1 ligand ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Costimulation through ICAM 1 resulted in a greater percentage of cells having undergone more than three divisions when compared to costimulation through leucocyte function associated antigen 1 ( LFA 1 ) . ^^^ Costimulation through ICAM 1 provided greater protection from apoptosis than costimulation through LFA 1 , especially in cells having divided more than three times . ^^^ Finally , costimulation through ICAM 1 or CD 28 produced a greater number of T cells with a memory phenotype than costimulation through LFA 1 . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In this study , we examined the distribution and kinetics of expression of E selectin , VCAM 1 , LFA 1 , and ICAM 1 in the NOD H2h4 mouse , a model of spontaneous autoimmune thyroiditis accelerated by dietary iodine . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The endothelial expression of ICAM 1 , VCAM 1 and E selectin was evaluated , as was the cellular expression of LFA 1 , VLA 4 and SLex . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Furthermore , inhibition of 9L cytotoxicity in assays that included blocking antibodies to MHC class 1 or to ICAM 1 revealed that T cell receptor ( TCR ) interactions with MHC class 1 and that ICAM 1 interactions with lymphocyte function associated 1 ( LFA 1 ) antigen account for a portion of the glioma lysis by aCTL . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Therefore , the role of the adhesion molecules intercellular adhesion molecule 1 ( ICAM 1 ) , LECAM 1 , CD 18 , leukocyte function associated antigen 1 ( LFA 1 ) and platelet endothelial cell adhesion molecule 1 ( PECAM 1 ) on the BjV induced neutrophil accumulation and the correlation with release of LTB 4 , TXA 2 , tumor necrosis factor alpha , interleukin ( IL ) 1 and IL 6 have been investigated . ^^^ This results suggest that ICAM 1 , LECAM 1 , LFA 1 and PECAM 1 , but not CD 18 , adhesion molecules are involved in the recruitment of neutrophils into the inflammatory site induced by BjV . ^^^ Anti mouse LECAM 1 , LFA 1 , ICAM 1 and PECAM 1 monoclonal antibody injection resulted in a reduction of 42 % , 80 % , 66 % and 67 % , respectively , of neutrophil accumulation induced by BjV ( 250 microg / kg , intraperitoneal ) injection in male mice compared with isotype matched control injected animals . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
This entry is facilitated by the up regulation of VCAM 1 and ICAM 1 on HBMECs and a corresponding increase of LFA 1 , VLA 4 , and MAC 1 on monocytes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
All starches regulate the expression of adhesion molecules LFA 1 and ICAM 1 , as well as receptor Mac 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
IL 3 , IL 5 , and GM CSF could enhance p 38 MAPK and NF kappaB activity and induce ICAM 1 , CD11b , and CD 18 expressions on eosinophils . ^^^ Therefore , the expression of ICAM 1 might involve both p 38 MAPK and NF kappaB activities , whereas the regulation of CD11b , CD 18 , and ICAM 3 expressions might be mediated through p 38 MAPK but not NF kappaB . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Our results show that whereas ICAM 1 and CD 18 molecules are both involved in homing to lymph nodes , neither is crucial for T cell bone marrow colonization . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
TCDD treatment modulated the expression of major histocompatibility complex ( MHC ) class 2 , CD 24 , ICAM 1 , CD 40 , and LFA 1 on splenic DC from C57Bl / 6 mice injected with allogeneic tumor cells ; however , the effects of TCDD were identical to changes seen previously in nonimmune mice , suggesting that these effects were not antigen dependent . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In addition , expression of the adhesion molecules leukocyte function associated antigen 1 ( LFA 1 , n = 6 ) , intercellular adhesion molecule 1 ( ICAM 1 , n = 11 ) , and L selectin ( n = 11 ) on CD34+ UCB cells was analyzed by flow cytometry after incubating the samples with either dimethyl sulfoxide ( DMSO ) 5 . 5 % , rhDNase 10 or 50 U / mL , or a combination of DMSO 5 . 5 % and rhDNase 50 U / mL . ^^^ The expression of adhesion molecules on untreated CD34+ UCB cells ( L selectin : 64 . 6 + / 18 . 8 % ; LFA 1 : 62 . 6 + / 7 . 5 % ; ICAM 1 : 14 . 8 + / 4 . 1 % ) did not show any significant difference compared with cells that were incubated with up to 50 U / mL rhDNase ( L selectin : 62 . 2 + / 19 . 3 % ; LFA 1 : 63 . 1 + / 5 . 9 % ; ICAM 1 : 17 . 5 + / 6 . 7 % ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The cells were exposed to 1 nm FMLP , 5 pm free ICAM 1 , or antibodies binding ICAM 1 or the beta 2 integrins CD11a , CD11b , CD11c and CD 18 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
A peptide derived from LFA 1 protein that modulates T cell adhesion binds to soluble ICAM 1 protein . ^^^ Leukocyte function associated antigen 1 ( LFA 1 ) and intercellular adhesion molecule 1 ( ICAM 1 ) have been shown to be critical for adhesion process and immune response . ^^^ Modulation or inhibition of the interaction between LFA 1 / ICAM 1 interactions can result in therapeutic effects . ^^^ Our group and others have shown that peptides derived from ICAM 1 or LFA 1 inhibit adhesion in a homotypic T cell adhesion assay . ^^^ It is likely that the peptides derived from ICAM 1 bind to LFA 1 and peptides derived from LFA 1 bind to ICAM 1 and inhibit the adhesion interaction . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The association of ICAM 1 with LFA 1 plays a critical role in several autoimmune diseases . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Flow cytometric analysis of paclitaxel or vinblastine treated P 815 cells revealed reduced cell surface expression of the adhesion molecules LFA 1 ( CD11a / CD18 ) and ICAM 1 ( CD 54 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
SKAP 55 enhances adhesion to fibronectin and intercellular adhesion molecule 1 ( ICAM 1 ) , colocalizes with actin at the T cell APC synapse and promotes the clustering of lymphocyte associated antigen 1 ( LFA 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In particular , they exhibit variable expression of the low affinity and non specific adhesion molecules LFA 1 and ICAM 1 , variable , clonally restricted and skewed expression of the TCR repertoire ( implying repeated antigenic stimulation possibly by CLL antigens ) , reduced CD 28 and CD 152 expression ( implying impairment of ability to start or stop an immune response ) and reduced IL 2 and CD 25 ( IL 2 R ) expression ( critical for positive feed back in maintenance and expansion of the T cell response to antigen presentation ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The expression of adhesion molecules ( CAMs ) such as VLA 4 , VLA 5 , LFA 1 , ICAM 1 , HCAM , L selectin and PECAM 1 , and the adhesion activity of the expanded CD ( 34 ) ( + ) cells were evaluated and compared with those of precultured fresh CD ( 34 ) ( + ) cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Cbl b / T cells also display increased adhesion and cell surface binding to ICAM 1 , a finding that is supported by the enhanced clustering of LFA 1 in Cbl b / T cells in response to TCR stimulation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
On various postoperative days , grafted rats were killed and mid eye sections were stained for expression of ICAM 1 ( CD 54 ) and beta ( 2 ) integrins ( CD 18 and CD11b / c ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Blocking LFA 1 or intercellular adhesion molecule 1 ( ICAM 1 ) significantly inhibited adhesion of D 4 cells to lung vascular endothelium and parenchyma of lung sections . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
AIM : Lymphocytes transiently express an active form of the beta _ 2 integrin LFA 1 ( LFA 1Af ) which has conformational changes in extracellular domains enabling higher affinity binding to the ligand ICAM 1 . ^^^ Furthermore , blocking studies demonstrate that a range of pathways are involved in this phenomenon ( LFA 1 / ICAM 1 , VLA4 / VCAM 1 , L selectin / E selectin ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To investigate whether TGZ acts by affecting the ICAM 1 / LFA 1 pathway and / or the Th1 / Th2 cytokine balance in NOD mice , we analysed the IL 1beta induced ICAM 1 expression on islet cells and the LFA 1 , CD 25 , IL 2 , IFN gamma , IL 4 , and IL 10 expression on splenocytes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Surface expression of leukocyte adhesion molecules Mac 1 ( CD11b / CD18 ) , LFA 1 ( CD11a / CD18 ) , L Selectin ( CD62L ) , ICAM 1 ( CD 54 ) , and MHC 2 ( HLA DR ) were assessed by flow cytometry . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Key among these molecules are P and E selectin , intercellular adhesion molecule 1 ( ICAM 1 ) and vascular cell adhesion molecule 1 ( VCAM 1 ) on the endothelial cells , and their respective counter receptors , P selectin glycoprotein ligand 1 ( PSGL 1 ) , leukocyte function associated antigen 1 ( LFA 1 ) and very late antigen 4 ( VLA 4 ) , on the leukocytes . ^^^ Clinical studies with humanised monoclonal antibodies which interfere with LFA 1 / ICAM 1 or VLA 4 / VCAM 1 interactions have shown significant efficacy and good safety profiles in autoimmune disease , including psoriasis , multiple sclerosis and inflammatory bowel disease . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Similarly , B cells showed no change in the expression of class 2 major histocompatibility antigens , LFA 1 , ICAM 1 , CD 19 , CD 40 , CD 80 , CD 86 , CD 95 ( Fas ) , or CD 178 ( FasL ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Targeting ICAM 1 / LFA 1 interaction for controlling autoimmune diseases : designing peptide and small molecule inhibitors . ^^^ This review describes the role of modulation of intracellular adhesion molecule 1 ( ICAM 1 ) / leukocyte function associated antigen 1 ( LFA 1 ) interaction in controlling autoimmune diseases or inducing immunotolerance . ^^^ ICAM 1 / LFA 1 interaction is essential for T cell activation as well as for migration of T cells to target tissues . ^^^ Therefore , blocking ICAM 1 / LFA 1 interaction can suppress T cell activation in autoimmune diseases and organ transplantation . ^^^ Many types of inhibitors ( i . e . antibodies , peptides , small molecules ) have been developed to block ICAM 1 / LFA 1 interactions , and some of these molecules have reached clinical trials . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The interaction of leukocyte function associated antigen 1 ( LFA 1 ) and intercellular adhesion molecule 1 ( ICAM 1 ) is central to the regulation of adhesion in leukocytes . ^^^ Enhanced adhesion of PMA stimulated cells to immobilized ICAM 1 stemmed from an increase in the number of LFA 1 ICAM 1 complexes formed between the two apposing surfaces on contact , rather than by affinity modulation of LFA 1 . ^^^ Single molecule force measurements revealed that the force spectrum of the LFA 1 ICAM 1 complex formed by PMA stimulated cells is identical to the force spectrum of the complex formed by resting cells . ^^^ Thus , PMA stimulation does not modify the mechanical strength of the individual LFA 1 ICAM 1 interaction . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Targeted disruption of the CD 18 or ICAM 1 gene inhibits choroidal neovascularization . ^^^ METHODS : Laser photocoagulation was used to induce CNV in wild type C57BL / 6J mice and species specific counterparts with targeted homozygous disruption of the CD 18 or ICAM 1 gene . ^^^ Expression of CD 18 and ICAM 1 after laser injury was assessed by immunostaining . ^^^ RESULTS : The site of laser injury showed upregulation of ICAM 1 and invasion by CD 18 positive leukocytes within 1 day of laser injury . ^^^ Significantly fewer lesions exhibited fluorescein leakage defined to be pathologically significant in CD 18 deficient mice at weeks 1 , 2 , and 4 weeks and in ICAM 1 deficient mice at 1 and 4 weeks , compared with the control . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The beta ( 2 ) integrin LFA 1 ( CD11a / CD18 ) is a leukocyte specific adhesion molecule that mediates leukocyte extravasation , antigen presentation , and T cell mediated cytolysis through its interaction with its counter receptors , ICAM 1 , ICAM 2 , and ICAM 3 . ^^^ We have recently described a small molecule antagonist of LFA 1 ( BIRT 377 ) that inhibits LFA 1 / ICAM 1 molecular interactions , LFA 1 dependent adhesion assays , antigen induced proliferation of T cells , and superantigen induced production of IL 2 in vivo in mice . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
LFA 1 induced T cell migration on ICAM 1 involves regulation of MLCK mediated attachment and ROCK dependent detachment . ^^^ This study analyzes signaling events initiated through binding of the leukocyte integrin LFA 1 to ICAM 1 , which leads to T cell attachment , polarization and random migration . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Expression of the integrins LFA 1 , VLA 4 and Mac 1 and of members of the immunoglobulin superfamily of adhesion molecules ICAM 1 and VCAM 1 was suppressed in the central nervous system ( CNS ) following rEPF treatment . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The regulation of ICAM 1 and LFA 1 interaction by autacoids and statins : a novel strategy for controlling inflammation and immune responses . ^^^ Some of the inhibitors of HMG CoA reductase were shown to bind to the ICAM 1 binding domain of LFA 1 , reducing the interaction mediated by ICAM 1 / LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
One day old DBA / 1 mice infected with Reo 2 intraperitoneally ( i . p . ) were treated i . p . with monoclonal antibodies ( mAbs ) against ICAM 1 and LFA 1 ( ICAM 1 / LFA 1 ) at 5 days post infection ( d . p . i . ) . ^^^ The present study suggests that ICAM 1 / LFA 1 may be required for the differentiation of Th 0 cells to Th 1 cells , which mediate insulitis with IGT in Reo 2 infected suckling mice . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
ICAM 1 ( intercellular adhesion molecule 1 ) , CD 18 ( beta chain of lymphocyte function associated antigen ) , LCA ( leukocyte common antigen ) , ED 1 ( monocyte / macrophage marker ) , and proliferating cell nuclear antigen expressions were evaluated in renal tissues 1 , 2 , and 7 weeks after injection . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Liver function parameters , blood cytokines ( IL 6 , IL 8 , and TNF alpha ) , expression of adhesion molecules ( ICAM 1 , ELAM 1 , LFA 1 and SleX ) , and immunoglobulins deposition in the graft were examined to evaluate the degree of immunological reaction . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The aim of this study was to investigate adhesion molecule expression ( ICAM 1 , ICAM 3 , VCAM 1 , LFA 1 and ELAM 1 ) by using an immunophosphatase alkaline ( APAAP ) technique in a double blind controlled study . ^^^ In group A , ICAM 1 positive cells decreased from 75 . 8 ( SE + / 15 . 12 ) to 38 . 8 ( SE + / 7 . 57 ) ICAM 3 positive cells decreased from 61 . 7 ( SE + / 12 . 72 ) to 45 . 2 ( SE + / 9 . 44 ) and LFA 1 decreased from 103 . 9 ( SE + / 17 . 34 ) to 66 . 5 ( SE + / 8 . 63 ) after cetirizine treatment ( p = 0 . 02 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Blocking ICAM 1 on C 8161 cells or Mac 1 on PMNs significantly inhibited C 8161 PMN adhesion and subsequent C 8161 migration through the endothelium under flow conditions . ^^^ These results suggest that transmigration of C 8161 cells under flow conditions can be influenced by PMNs , mediated by Mac 1 / ICAM 1 adhesive interactions and enhanced by altered cytokine production . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Therefore , binding of LFA 1 to ICAM 1 on target cells may initiate an early signaling cascade in NK cells through activation of Vav 1 , leading to cytoskeleton reorganization and amplification of signals from other activation receptors . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Human RAPL stimulated lymphocyte polarization and the patch like redistribution of lymphocyte function associated antigen 1 ( LFA 1 ) to the leading edge , resulting in enhanced adhesion to intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We have produced by phage display a disulfide linked cyclic nonapeptide ( inhibitory peptide 01 , IP 01 ) , CLLRMRSIC , that binds to intracellular adhesion molecule 1 ( ICAM 1 ) and blocks binding to its counter structure , leukocyte functional antigen 1 ( LFA 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
OBJECTIVE : To evaluate the adhesion of acute myeloid leukemia ( AML ) blasts to human umbilical vein endothelial cells ( HUVECs ) and the roles of intercellular adhesion molecule 1 ( ICAM 1 ) and its ligand lymphocyte function associated antigen 1 ( LFA 1 ) in binding of leukemic blasts to HUVECs . ^^^ We also observed the adhesion of leukemic blasts in the presence of the adhesion blocking mAbs anti ICAM 1 and anti LFA 1 . ^^^ Treatment of HUVECs with AML blasts supernatant for 24 hours increased the expression of ICAM 1 ( 65 . 36 + / 5 . 97 ) % , ( 1 . 424 + / 0 . 469 ) microg / L respectively ( n = 21 , P < 0 . 05 ) and anti ICAM 1 and anti LFA 1 significantly inhibited the adhesion of AML blasts attachment to TNF alpha activated endothelial cell monolayers ( 20 . 12 + / 1 . 73 ) % , ( n = 10 , P < 0 . 001 ) . ^^^ CONCLUSION : Our results indicate that leukemic blasts have the ability to generate some factors that stimulate endothelial cell to secrete ICAM 1 ane to promote their own adhesion to vascular endothelium , interaction of ICAM 1 and its ligand LFA 1 has a key role in adhesion of leukemic blasts and HUVECs . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The present study attempts to determine if APC inactivation induces changes in the cross talk between IEC and large intestine IEL ( LI IEL ) through intercellular adhesion molecule ( ICAM 1 ) / leukocyte function associated ( LFA 1 ) interactions . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Antibodies specific for ICAM 1 and LFA 1 inhibited both cooperative islet destruction and cross stimulation of CD8+ T cells and macrophages . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Function blocking anti CD 18 and anti intercellular adhesion molecule 1 ( ICAM 1 ) antibodies significantly reduced activated monocyte induced TUNEL positive HRPE cells by 48 % ( p = 0 . 0051 ) and 38 % ( p = 0 . 046 ) , respectively . ^^^ Anti CD 18 and anti ICAM 1 antibodies significantly inhibited caspase 3 activity by 56 % ( p < 0 . 0001 ) and 45 % ( p < 0 . 0001 ) , respectively . ^^^ The intracellular HRPE cell ROM production was inhibited by the anti CD 18 and anti ICAM 1 antibodies , but not by superoxide dismutase , presumably due to its failure to penetrate into HRPE cells . ^^^ Our results suggest that activated monocytes may induce ROM in HRPE cells through cell to cell contact , in part via CD 18 and ICAM 1 , and promote HRPE cell apoptosis . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Virion bound ICAM 1 and activated LFA 1 : a combination of factors conferring resistance to neutralization by sera from human immunodeficiency virus type 1 infected individuals independently of the disease status and phase . ^^^ The role of the supplementary interaction between virion bound host ICAM 1 and LFA 1 on target cells in sensitivity to neutralization of human immunodeficiency virus type 1 ( HIV 1 ) is poorly studied . ^^^ We found that virus neutralization sensitivity to the studied sera is not modified by the additional interaction between virally embedded ICAM 1 and LFA 1 under an inactive state . ^^^ However , expression on the target cell surface of an activated LFA 1 form renders ICAM 1 bearing virus particles , but not viruses devoid of ICAM 1 , more refractory to neutralization by sera from three out of four LTNPs and all sequential sera from the person who has experienced a progression of the HIV 1 associated disease . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Expression of ICAM 1 and LFA 1 molecules in relation to renal allograft rejection in rats . ^^^ OBJECTIVE : The purpose of this study was to assess the renal graft expression of ICAM 1 ( intercellular adhesion molecule 1 ) and LFA 1 ( lymphocyte function associated antigen 1 ) molecule with relation to graft rejection . ^^^ The sections of renal graft were stained for monoclonal antibody ICAM 1 and LFA 1 , and then quantification of ICAM 1 and LFA 1 expression was accomplished by computer image analysis . ^^^ RESULTS : ICAM 1 and LFA 1 increased significantly in the renal allograft rejection group as compared with the non rejection groups ( P < 0 . 05 ) . ^^^ CONCLUSION : Both biopsy of renal graft and monitoring of ICAM 1 and LFA 1 are useful tools in diagnosing and treating acute rejection . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The killing requires the binding of MM to VSMC via Mac 1 ( CD11b / CD18 ) on MM and intracellular adhesion molecule 1 ( ICAM 1 ) on VSMC . ^^^ CONCLUSIONS : MM and M CSF induced VSMC killing requires MM binding to VSMC mediated by Mac 1 and ICAM 1 , and Fas FasL interaction . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
These cells were also ICAM 1 ( CD 54 ) and LFA 1 ( CD11a ) positive . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Selective immunosuppression with monoclonal antibodies against ICAM 1 and LFA 1 with FK 506 after experimental small intestine transplantation in the rat ] . ^^^ In this study anti ICAM 1 ( 1A29 ) and anti LFA 1 ( WT 1 ) mabs were used in combination with a subtherapeutic dose of FK 506 for 13 days after allogeneic SBT in the rat . ^^^ The results of this study indicate that in contrast to the induction of allospecific tolerance after liver transplantation , the addition of anti ICAM 1 and / or of anti LFA 1 mabs after SBT reverses the FK 506 effect and results in early graft rejection . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Furthermore , high ICAM 1 expression may be associated with the presence of multiorgan manifestations of CMA , whereas high LFA 1 expression may reflect the IgE mediated disease . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Trapping of lymphocytes in the lung after i . v . injection is partly mediated by LFA 1 / ICAM interactions ; the data presented in this study show an exclusive role of ICAM 1 in LFA 1 dependent lung trapping . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In conclusion , this study revealed a significant association between the G / R241 ICAM 1 polymorphism and serum sICAM 1 levels , probably due to the impairment in binding of ICAM 1 to leukocyte integrin Mac 1 protein . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
It is well known that the inhibition of adhesion is very effective in immunotherapy and that the peptides derived from leukocyte function associated antigen ( LFA 1 ) and intercellular adhesion molecule ( ICAM 1 ) modulate cell adhesion interaction . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Immunoneutralization of LFA 1 and ICAM 1 abolished leukocyte adhesion , whereas inhibition of VLA 4 had no effect in this in vitro assay . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
An immunophenotype analysis of the plasma cells showed positivity of cytoplasmic IgM , 10 , CD 5 , CD 38 , CD11a ( LFA 1 ) , CD 44 ( HCAM ) , CD49d ( VLA 4 ) and CD 54 ( ICAM 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The purpose of this study was to evaluate the effect of hyperbaric oxygen on a rat musculocutaneous flap versus ischemia reperfusion injury , focusing on the mechanism involving the expression of adhesion molecules such as intercellular adhesion molecule 1 ( ICAM 1 ) of endothelial cells and CD 18 of neutrophils . ^^^ The study consisted of gross examination for flap survival , histology , immunohistochemical staining , myeloperoxidase assay , flow cytometric study of CD 18 , and Northern blot analysis on ICAM 1 messenger ribonucleic acid expression . ^^^ These results suggests that hyperbaric oxygen reduces the accumulation of leukocytes in the TRAM flap , but not enough to prevent adhesion of neutrophils on endothelial cells ; ischemia reperfusion injury increases the expression of CD 18 and ICAM 1 and causes increased adhesion of leukocytes on the endothelium ; hyperbaric oxygen does not alter the expression of CD 18 but decreases the expression of ICAM 1 ; and the point of application for hyperbaric oxygen , whether applied before or after reperfusion , did not show any differences in outcome . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In addition , the level of ICAM 1 expression on sinusoidal endothelial cells greatly increased from 8 h after exposure to endotoxin , and these cells were adhered to neutrophils that expressed both LFA 1 and Mac 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Using flow cytometry analysis , the co addition of Fe citrate significantly suppressed CD 18 ( beta 2 integrin ) and CD 54 ( ICAM 1 ) but not CD11a ( alpha integrin ) expression on Con A stimulated monocytes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
OBJECTIVE : This study examined the role of intercellular adhesion molecule 1 ( ICAM 1 ) and lymphocyte function associated antigen 1 ( LFA 1 ) in the autoimmune process of bile duct destruction in the early stages of primary biliary cirrhosis ( PBC ) . ^^^ Immunohistochemical studies of ICAM 1 and LFA 1 , and Western blot of ICAM 1 were performed . ^^^ Immunoelectron microscopy localized ICAM 1 on the luminal and basal surfaces as well as on lymphocytes around damaged bile duct epithelial cells , and LFA 1 on lymphocytes around damaged bile ducts . ^^^ Messenger RNA expression of ICAM 1 was demonstrated in bile ducts , and LFA 1 in lymphocytes around bile ducts . ^^^ CONCLUSION : De novo expression of ICAM 1 and LFA 1 at protein and mRNA levels in PBC may imply an inductive role of ICAM 1 through binding with its ligand LFA 1 in the extravasation of activated lymphocytes and lymphocyte mediated bile duct destruction . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Lymphocyte function associated antigen 1 ( LFA 1 ) , macrophage 1 molecule ( Mac 1 ) and very late appearing antigen 4 ( VLA 4 ) , all ligands of ICAM 1 and VCAM 1 , were present on the transmigrated neutrophils and macrophages . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Thus , we investigated , with confocal microscopy , the distribution dynamics of the central adhesion molecules ICAM 1 and LFA 1 in this context . ^^^ Monolayers of activated HUVECs stained with fluorescent anti ICAM 1 Fabs or Chinese hamster ovary K 1 cells expressing ICAM 1 green fluorescent protein were allowed to bind LFA 1 bearing monocytes , neutrophils , or K 562 LFA 1 transfectants . ^^^ ICAM 1 was rapidly relocalized to newly formed microvilli like membrane projections in response to binding LFA 1 on leukocytes . ^^^ These ICAM 1 enriched projections encircled the leukocytes extending up their sides and clustered LFA 1 underneath into linear tracks . ^^^ These data show that in response to LFA 1 engagement the endothelium proactively forms an ICAM 1 enriched cup like structure that surrounds adherent leukocytes but is not important for firm adhesion . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We examined the expression and dynamics of interleukin 1beta ( IL 1beta ) , tumor necrosis factor alpha ( TNF alpha ) , monocyte chemotactic protein 1 ( MCP 1 ) , macrophage inflammatory protein 1alpha ( MIP 1alpha ) , MIP 2 , GRO / cytokine induced neutrophil chemoattractant 2alpha ( CINC 2alpha ) , intercellular adhesion molecule 1 ( ICAM 1 ) , leukocyte function associated antigen 1 ( LFA 1 ; CD11a / CD18 ) , and Mac 1 ( CD11b / CD18 ) in rat chronic reflux esophagitis . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Mechanism of binding and internalization of ICAM 1 derived cyclic peptides by LFA 1 on the surface of T cells : a potential method for targeted drug delivery . ^^^ PURPOSE : Peptides derived from the Domain 1 of the adhesion molecule ICAM 1 ( 1 21 ) are being developed as targeting ligands for LFA 1 receptors expressed on activated T cells . ^^^ This work aims to elucidate the binding and internalization of ICAM 1 derived cyclic peptides ( cIBL , cIBC , and cIBR ) to LFA 1 . ^^^ Inhibition of ICAM 1 binding to LFA 1 by peptides was evaluated using a Biacore assay . ^^^ However , the binding properties of the soluble forms of LFA 1 and ICAM 1 may not correlate to their interaction at the cell surface . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In this study , we used mice deficient in ICAM 1 ( ICAM 1 ( null ) ) to study the function of ICAM 1 as an endothelial ligand for Mac 1 and LFA 1 . ^^^ Similarly , leukocyte adhesion efficiency in ICAM 1 ( null ) mice ( 0 . 11 + / 0 . 01 mm ) was similar to that in Mac 1 ( / ) ( 0 . 12 + / 0 . 03 mm ) mice but significantly increased compared with that in LFA 1 ( / ) ( 0 . 08 + / 0 . 01 mm ) mice and significantly reduced from that in wild type ( 0 . 26 + / 0 . 04 mm ) . ^^^ When both LFA 1 and ICAM 1 were blocked , rolling velocity increased , and adhesion efficiency and arrest decreased . ^^^ We conclude that endothelial ICAM 1 is the main ligand responsible for slow leukocyte rolling mediated by Mac 1 , but not LFA 1 . . ^^^ However , blocking both Mac 1 and ICAM 1 had no greater effect than either blockade alone . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In immature DC , failure to form podosomes or selective inhibition of the CD 18 component of podosomes resulted in a similarly reduced ability to adhere to ICAM 1 , indicating that podosomes , through CD 18 , are necessary for tight adhesion to this ligand . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
METHODS AND RESULTS : The expression of leukocyte ( ie , neutrophil , monocyte and lymphocyte ) surface CD11a , CD 18 , intracellular adhesion molecule 1 ( ICAM 1 ) , very late antigen 4 alpha ( VLA 4 alpha ) and L selectin was investigated by flow cytometry in 20 patients who were initially diagnosed with CAD ( CAD group ) , 15 patients with coronary re stenosed vessels ( RESTE group ) , 20 undergoing HD ( HD group ) and 20 without CAD ( CONT group ) . ^^^ CONCLUSIONS : Because CD11a and CD 18 are expressed on the cell surface as a heterodimer and ICAM 1 is a ligand for CD11a / CD18 , this increased expression of CD11a and ICAM 1 may affect the development of initial atherosclerotic coronary stenosis , but not re stenosis . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NPC transplantation attenuated the clinical severity of EAE and the brain inflammation , indicated by reduction in perivascular infiltrates and decreased expression of ICAM 1 and LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Immunohistochemical stains using the MoAbs HMB 45 , CD 4 , CD 8 , ICAM 1 , and LFA 1 were performed in all biopsies and the labelled cells were counted by a digital image analyser . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The absence of ICAM 1 expression on infected keratinocytes may reduce their antigen presentation capacities to LFA 1 ligand bearing T cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
AIM : To investigate the anti inflammatory mechanism of esculentoside A ( EsA ) and to observe the effects of EsA on cellular adhesion between human umbilical vein endothelial cell ( VEC 304 ) and human neutrophil and to further observe the mRNA expression of intercellular adhesion molecule 1 ( ICAM 1 ) and cluster of differentiation 18 ( CD 18 ) . ^^^ The RT PCR method was used for measuring the mRNA expression of ICAM 1 and CD 18 . ^^^ The results of RT PCR from VEC 304 and human neutrophil treating with LPS showed that ICAM 1 and CD 18 mRNA expressions were higher than those of normal cells , while this increased expression of ICAM 1 and CD 18 mRNA was remarkably attenuated by the addition of EsA . ^^^ Moreover , LPS induced high expression of ICAM 1 and CD 18 was inhibited with treatment of EsA . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Rolling cells sample the vascular surface for chemoattractant compounds ( e . g . , thymus and activation regulated chemokine / CCL17 interacting with CCR 4 on the leukocyte surface ) and , if successfully stimulated , progress to firm arrest and transmigration mediated by LFA 1 and vascular ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Comparison of the kinetics of virus replication and B and T lymphocyte infiltration in mice lacking key adhesion molecules ( L selectin ( L sel ( / ) ) , P selectin ( P sel ( / ) ) , intracellular adhesion molecule 1 ( ICAM 1 ( / ) ) , or leukocyte function associated antigen 1 ( LFA 1 ( / ) ) ) demonstrates a role for individual adhesion molecules in recruitment of immune cells into central nervous system ( CNS ) , but the role is not significant to prevent eventual virus clearance . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
CONCLUSION : These data suggest that SOM treatment of spleen cells , especially in CD8+ T cells , leads to downregulation of LFA 1 mRNA translation , inside out signaling molecules for integrins ( Ras , Rap 1 and PI 3 kinase , but not PKC ) , and consequently to a decrease in the LFA 1 mediated adhesion to ICAM 1 . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We provide evidence that the prevalence of a certain T cell subset inside the inflamed heart reflects the differential profile of the adhesion molecules VLA 4 , LFA 1 , and ICAM 1 displayed on a large proportion of this particular T cell population in peripheral blood during the early phase of inflammation . ^^^ Further , the expression of VCAM 1 , ligand for VLA 4 , and ICAM 1 , counter receptor for LFA 1 , was up regulated on vascular endothelium and paralleled the entrance of inflammatory cells into the cardiac tissue . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Anti CD 18 and anti ICAM 1 antibodies significantly reduced activated monocyte induced TUNEL positive HRPE cells , by 48 % ( P = . 0051 ) and 38 % ( P = . 046 ) , respectively , and caspase 3 activity by 56 % ( P < . 0001 ) and 45 % ( P < . 0001 ) , respectively . ^^^ Overlay of monocytes induced HRPE cell ROM that was inhibited by anti CD 18 and anti ICAM 1 antibodies , but not by superoxide dismutase ( SOD ) or nitric oxide ( NO ) inhibitors . ^^^ CONCLUSIONS : We demonstrated that IFN gamma activated monocytes may induce ROM in HRPE cells through cell to cell contact , in part via CD 18 and ICAM 1 , and promote HRPE cell apoptosis via caspase 3 activation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We suggest that random trafficking of activated T cells ( both Th 1 and Th 2 ) across the blood retina barrier is mediated by CD 44 : CD44R and LFA 1 : ICAM 1 , whereas preferential recruitment of Th 1 cells is mediated by PSGL 1 : P / E selectin . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Effect of LFA 1 expression on neutrophils and serum level of soluble ICAM 1 in patients with chronic myelogenous leukemia ] . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In a comparison of LFA 1 ligands , intercellular adhesion molecule 2 ( ICAM 2 ) and ICAM 3 promoted LFA 1 directed perforin release , whereas ICAM 1 had little effect . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Immunohistochemical staining showed that P 1 and P 2 could mimic the binding of ICAM 1 to its receptor LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
ICAM 1 on endothelial cells mediates leukocyte adhesion by binding to leukocyte function associated antigen 1 ( LFA 1 ) and macrophage antigen 1 ( Mac 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Compounds 1 and 2 inhibited PMA induced ICAM 1 / LFA 1 mediated homotypic aggregation of the HL 60 cells without cytotoxicity with MIC values of 1 . 0 and 5 . 5 nM , respectively . ^^^ Even though 1 and 2 did not affect the adhesion of ICAM 1 to LFA 1 , these compounds inhibited PMA induced ICAM 1 expression in HL 60 cells in a dose dependent fashion . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We pulsed cells of monocytoid lineage ( established and primary ) and CD 4 negative epithelial cells transiently expressing DC SIGN or LFA 1 with isogenic HIV 1 particles either devoid or bearing host derived ICAM 1 or ICAM 3 before incubation with an indicator cell line . ^^^ To our surprise , the ICAM 1 / LFA 1 association was a more efficient transmission factor than the combined gp120 / DC SIGN and ICAM 3 / DC SIGN interactions . ^^^ The involvement of the association between virus bound ICAM 1 and its natural ligand LFA 1 in virus binding and carriage was confirmed when using more physiological cellular targets , i . e . , human lymphoid tissues cultured ex vivo . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Retinal leukostasis increases within days of developing diabetes and correlates with the increased expression of retinal intercellular adhesion molecule 1 ( ICAM 1 ) and CD 18 . ^^^ Mice deficient in the genes encoding for the leukocyte adhesion molecules CD 18 and ICAM 1 were studied in two models of diabetic retinopathy with respect to the long term development of retinal vascular lesions . ^^^ CD 18 / and ICAM 1 / mice demonstrate significantly fewer adherent leukocytes in the retinal vasculature at 11 and 15 months after induction of diabetes with STZ . ^^^ These changes are significantly reduced in CD 18 and ICAM 1 deficient mice . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Direct quantification of the modulation of interaction between cell or surface bound LFA 1 and ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Directly fluorescence conjugated antibodies that were directed against adhesion molecules ( LFA 1 ( CD11a ) , HCAM ( CD 44 ) , VLA 2 ( CD49b ) , ICAM 1 ( CD 54 ) , NCAM ( CD 56 ) , LECAM 1 ( CD62L ) and CD 86 ) were used . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Mouse cremaster venules are predisposed to light / dye induced thrombosis independent of wall shear rate , CD 18 , ICAM 1 , or P selectin . ^^^ Similarly , in animals deficient in CD 18 , P selectin , or ICAM 1 , the time to form platelet thrombi in arterioles was > 3 . 0 fold higher than in venules . ^^^ The data suggest that functional differences between arteriolar and venular endothelial cells ( independent of wall shear rate and of CD 18 , P selectin , and ICAM 1 ) account for the venular predisposition to thrombosis . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We demonstrate that peptide / MHC ( pMHC ) tetramers rapidly enhance LFA 1 dependent adhesion of OT 1 TCR transgenic CD 8 ( + ) T cells to purified ICAM 1 . ^^^ The Ag thresholds required for T cell adhesion to ICAM 1 resemble those of early T cell activation events , because optimal LFA 1 activation occurs at tetramer concentrations that fail to induce maximal T cell proliferation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
By using monoclonal antibodies and recombinant intercellular adhesion molecule 1 ( ICAM 1 ) to engage lymphocyte function associated antigen 1 ( LFA 1 ) on monocytic cells , we found that the cellular adhesion to vascular cell adhesion molecule 1 ( VCAM 1 ) mediated by very late antigen 4 ( VLA 4 ) was suppressed after this treatment and the suppression depended on the presence of reactive oxygen species ( ROSs ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Similar aberrant patterns of LFA 1 / ICAM 1 engagement in live T B couples correlate with reduced calcium flux and IL 2 secretion . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Effect of ligustrazine on the expression of LFA 1 , ICAM 1 following bone marrow transplantation in mice . ^^^ The effects of ligustrazine on the expression of LFA 1 , ICAM 1 in bone marrow tissue and the mechanism promoting hematopoietic reconstitution following bone marrow transplantation ( BMT ) were investigated . ^^^ At the 7th , 14th , 21st and 28th day after BMT , survival rate , colony forming unit of spleen ( CFU S ) , peripheral blood cells and bone marrow mononuclear cells ( BMMNC ) were measured , histological changes in bone marrow tissue were observed and the expression level of LFA 1 , ICAM 1 was detected . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Interaction between ICAM 1 , present on endothelial cells , and LFA 1 facilitates leukocyte adhesion and migration across the endothelium . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Neutrophil attachment was mainly CD 18 integrins mediated and depended on P selectin , but not on the endothelial adhesion molecules E selectin , ICAM 1 or PECAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In the present work we have investigated , by flow cytometric techniques , the expression of the two major integrins CD11a ( LFA 1 ) and CD49d ( VLA 4 ) that are the homing receptor cognate for ICAM 1 and VCAM 1 on human cord blood CD 34 hematopoietic progenitor and stem cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The role of LFA 1 was evaluated by exposing NK cells to human ICAM 1 that was either expressed on a Drosophila insect cell line , or directly coupled to beads . ^^^ Expression of ICAM 1 on insect cells was sufficient to induce lysis by NK cells through LFA 1 . ^^^ Coexpression of peptide loaded HLA C with ICAM 1 on insect cells blocked the LFA 1 dependent cytotoxicity of NK cells that expressed HLA C specific inhibitory receptors . ^^^ Polarization of cytotoxic granules in NK cells toward ICAM 1 and ICAM 2 coated beads showed that engagement of LFA 1 alone is sufficient to initiate activation signals in NK cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We have previously described a disulfide linked cyclic nonapeptide ( inhibitory peptide 01 , IP 01 ) , with the sequence CLLRMRSIC , which binds to intercellular adhesion molecule 1 ( ICAM 1 ) , and blocks binding to its counter structure , the integrin alphaLbeta 2 ( leukocyte functional antigen 1 , LFA 1 ) ( Sillerud et al . , J . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The increase in costimulatory ( CD 80 and CD 86 ) and adhesion ( ICAM 1 , PGP 1 , LFA 1 , and Mac 1 ) in peripheral blood mononuclear cells was also attenuated by food restriction and to a lesser extent by fish oil alone . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Recent advance on LFA 1 / ICAM 1 costimulatory signal of T cell review ] . ^^^ LFA 1 / ICAM 1 costimulation plays an important role in immunologic reaction of many different T cell populations . ^^^ After TCR / CD3 complex cross linking MHC / peptide , LFA 1 , expressed on T cell increases a higher affinity and avidity for ICAM 1 rapidly . ^^^ LFA 1 / ICAM 1 costimulation can engage various signaling events of T cell by up regulating the activities of PI 3 kinase , sphingomyelinase , and c Jun NH 2 terminal kinase . ^^^ With the costimulation of LFA 1 / ICAM 1 , engagement of TCR molecules results in a significant increase of T cell activities , including higher Th 1 cytokines production , strongly proliferative response and higher T cell cytotoxicity . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Our previous study showed that the integrin beta ( 2 ) specific monoclonal antibody 7E4 abrogates monoclonal antibody KIM 185 activated but not Mg ( 2+ ) / EGTA activated leukocyte function associated antigen 1 ( LFA 1 ; alpha ( L ) beta ( 2 ) ) mediated adhesion to ICAM 1 . ^^^ Two sets of constitutively active LFA 1 variants were used to examine the effect of 7E4 on LFA 1 / ICAM 1 binding . 7E4 attenuated the binding of variants that have modifications to regions membrane proximal with respect to the beta ( 2 ) hybrid domain . ^^^ Furthermore , 7E4 abrogated LFA 1 / ICAM 1 adhesion of phorbol 12 myristate 13 acetate treated MOLT 4 cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Examples include the integrin intercellular adhesion molecule 1 ( ICAM 1 ) , which , once acquired by HIV 1 , promotes virus infectivity via ligation to LFA 1 . ^^^ We tested the ability of statins to diminish HIV 1 replication , based on the idea that these compounds have been shown to block ICAM 1 LFA 1 interactions . ^^^ Our data indicate that statins diminish HIV 1 attachment to target cells by suppressing ICAM 1 LFA 1 interactions . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Expression of CD 8 , B 7 2 ( CD 86 ) , and to lesser extent MHCII , LFA 1 ( CD11a ) , and ICAM 1 ( CD 54 ) , was significantly enhanced at the invasion front of control tumors after LITT compared to hepatic resection . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Co expression of E selectin and ICAM 1 boosted adhesion efficiency threefold more than either receptor alone over the range of active CD 18 expression . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
A number of receptor / ligand pairs have been shown to mediate costimulation , including CD28 / B7 molecules ( CD 80 and CD 86 ) , CD40 / CD40 ligand ( CD40L , CD 154 ) , and LFA 1 ( CD 18 ) / ICAM 1 ( CD 54 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
BIS20x3 mediated T cell B cell interaction resulted in a significant up regulation of ICAM 1 on B cells and the activation of T cells was found to be dependent on the interaction of ICAM 1 with LFA 1 and trans activation by the NF kappaB pathway . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
It increases the expression of adhesion molecules LFA 1 , ICAM 1 , and costimulatory molecule B 7 1 of B cells , and regulates the antibody and cytokine secreted by B cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The list of such targets includes the ligand pairs CD 28 : B 7 , CD 154 : CD 40 , LFA 1 : ICAM 1 , ICOS : B7RP 1 , and VLA 4 : VCAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Our results suggest that transmigration of C 8161 cells under flow conditions can be influenced by PMNs , mediated by Mac 1 and ICAM 1 adhesive interactions and enhanced by altered cytokine production . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
RESULTS : Good mobilizing patients , defined as those who collected their target in one apheresis procedure , had significantly fewer cells that expressed CD11a ( LFA 1 ) and CD 54 ( ICAM 1 ) and borderline fewer that expressed CD11c , CD49d ( VLA 4 ) , and CD49d ( VLA 5 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The expression of adhesion molecules ( alpha4beta1 integrin , LFA 1 , ICAM 1 ) on T cells , measured by flow cytometry , was compared in different subtypes of multiple sclerosis ( MS ) and related to future lesion development as seen as delta T 1 and T 2 lesion load per year on magnetic resonance imaging ( MRI ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We recently reported that activated T cells induce mast cell degranulation with the release of TNF alpha , and that this activation pathway is mediated by lymphocyte function associated antigen 1 ( LFA 1 ) / intercellular adhesion molecule 1 ( ICAM 1 ) binding . ^^^ RESULTS : Co culturing HMC 1 with activated , but not with resting T cells resulted in marked stimulation of mast cell adhesion to vascular cell adhesion molecule 1 and ICAM 1 in a very late antigen 4 and LFA 1 dependent fashion . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Early effect of Mycobacterium tuberculosis infection on Mac 1 and ICAM 1 expression on mouse peritoneal macrophages . ^^^ Effect of M . tuberculosis infection was studied on the expression of intercellular adhesion molocule 1 ( ICAM 1 ) and Mac 1 markers on murine peritoneal macrophages . ^^^ Intraperitoneal administration of M . tuberculosis resulted in a marked increase in the proportion of Mac 1 ( + ) cells whereas the proportion of ICAM 1 ( + ) cells declined sharply 4 h post infection . ^^^ Absolute numbers of Mac 1 ( + ) and ICAM 1 ( + ) cells however increased at all time points after the infection . ^^^ Comparison of kinetics of changes observed in Mac 1 ( + ) and ICAM 1 ( + ) cell populations with differential leukocyte counts in peritoneal cells indicated that these alterations could be due to cellular influx , especially that of neutrophils , or up regulation of these markers on macrophages and other peritoneal cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Blood lymphocytes , monocytes and NK cells modulate their expression of CD 44 , ICAM 1 , LFA 1 and MHC class 2 after arrival into lymphoid organs . ^^^ The helper and cytotoxic subsets of T cells , as well as B cells , display the increased expression of CD 44 , ICAM 1 and LFA 1 , whereas B cells additionally show the augmented expression of MHC class 2 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Reduction of ICAM 1 and LFA 1 positive leukocytes in the perivascular space of arteries under mycophenolate mofetil therapy reduces rat heart transplant vasculopathy . ^^^ BACKGROUND : The interaction of lymphocyte function associated antigen 1 ( LFA 1 ) positive host leukocytes with intercellular adhesion molecule 1 ( ICAM 1 ) on graft endothelium may play a key role in allograft recognition , triggering the development of transplant vasculopathy ( TVP ) . ^^^ We investigated the correlation between TVP and ICAM 1 expression and accumulation of LFA 1 positive leukocytes in the perivascular space ( PVS ) of arteries under different immunosuppressive drugs . ^^^ RESULTS : Accumulation of LFA 1 positive leukocytes in the PVS of arteries and the myocardium correlated with expression of ICAM 1 on graft endothelium . ^^^ All immunosuppressive drugs significantly reduced ICAM 1 expression , LFA 1 accumulation and extent of TVP , compared with controls . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Coordinated redistribution of leukocyte LFA 1 and endothelial cell ICAM 1 accompany neutrophil transmigration . ^^^ Endothelial ICAM 1 and JAM A both colocalized with the ringlike LFA 1 cluster . ^^^ Further analysis of PMA stimulated neutrophils , which increase mobility of LFA 1 , showed a rapid redistribution of LFA 1 and ICAM 1 , but not endothelial JAM A . ^^^ This is the first demonstration that neutrophil LFA 1 rapidly redistributes to form a ringlike structure that coclusters with endothelial ICAM 1 as the neutrophil transmigrates . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Binding of monomeric intercellular adhesion molecule 1 ( ICAM 1 ) induced profound changes in the conformation of LFA 1 but did not alter clustering , whereas binding of ICAM 1 oligomers induced significant microclustering . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The fluorescence of CD 18 and CD11b reappeared on the cell membrane 1 h after re treatment with PMA , suggesting the recycling of non degraded Mac 1 . ( 2 ) The adhesion rate of CHO Mac 1 FP to magnetic beads coupled ICAM 1 increased within 4 h after their initial interaction , accompanied by the clustering of Mac 1 FP . ^^^ The pattern of change in fluorescence in CHO Mac 1 FP cells elicited by ICAM 1 beads was similar to that elicited by PMA , suggesting that endocytosis and degradation of Mac 1 occurred after the interaction with ICAM 1 . ^^^ These changes are in parallel with the adhesion of CHO Mac 1 FP cells with ICAM 1 , and may be involved in the adhesion and detachment of leukocytes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Rolling of Th 1 cells via P selectin glycoprotein ligand 1 stimulates LFA 1 mediated cell binding to ICAM 1 . ^^^ In this study , we show that Ab mediated cross linking of the PSGL 1 on Th 1 cells enhances LFA 1 dependent cell binding to ICAM 1 . ^^^ PSGL 1 cross linking did not enhance soluble ICAM 1 binding but induced clustering of LFA 1 on the cell surface , suggesting that an increase in LFA 1 avidity may account for the enhanced binding to ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
A plausible model was proposed , in which all polar atoms of ( d ) 1 are linked by hydrogen bonds or electrostatic interactions with the functional residues of ICAM 1 , that have been supposed to be necessary for the binding with LFA 1 ( leukocyte function associated antigen 1 ) . ^^^ This suggests the potent inhibitory activity of 1 for the ICAM 1 / LFA 1 adhesion and would be important on developing the clinically usable drugs for the inflammatory diseases . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Little is known about the cross talk between ICAM 1 , expressed on epithelium , endothelium , and APC , and its ligand , LFA 1 , at the feto maternal interface . ^^^ However , based on the role of ICAM 1 / LFA 1 in allograft acceptance or rejection upon transplantation , adhesion molecules are likely to interfere with successful pregnancy outcome . ^^^ In this study , we tested the hypothesis that ICAM 1 / LFA 1 pathways may be involved in pregnancy rejection in murine models . ^^^ By blocking ICAM 1 / LFA 1 mediated intercellular adhesion events , we show that fetal immune acceptance is restored in challenged pregnancies ( e . g . , upon exposure to sound stress ) , and adoptive transfer of LFA 1 cells into pregnant mice induces rejection only in abortion prone mouse models . ^^^ ICAM 1 / LFA 1 cross talk leads to increased recruitment of proinflammatory cells to the implantation site , promotes dendritic cell maturation in the decidua , and subsequently induces additional local Th 1 polarization via mature dendritic cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
SGHPL 4 cells were shown to express HLA G , alpha4beta1 and ICAM 1 at high levels and LFA 1 and VCAM 1 at lower levels . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Trophozoite adherence to endothelial cells expressing ICAM 1 ( CD 54 ) following activation with TNF alpha or ICAM 1 transfected CHO cells was specifically inhibited with anti CD 18 or anti CD 54 MAbs . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
RESULTS : I / R plasma ( withdrawn 15 min following tourniquet release ) increased isolated neutrophil CD11b ( P = 0 . 03 ) , CD 18 ( P = 0 . 01 ) and endothelial intercellular adhesion molecule 1 ( ICAM 1 ) ( P = 0 . 008 ) expression compared to baseline . ^^^ CD11b , CD 18 and ICAM 1 expression on lidocaine ( 0 . 005 mg mL ( 1 ) ) treated neutrophils was similar to control . ^^^ CD11b ( P < 0 . 001 ) , CD 18 ( P = 0 . 03 ) and ICAM 1 ( P = 0 . 002 ) expression on lidocaine ( 0 . 05 mg mL ( 1 ) ) treated neutrophils and HUVECs was less than that on controls . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
METHODS : Isolated human neutrophil CD11b and CD 18 , and human umbilical vein endothelial cell ( HUVEC ) ICAM 1 expression and supernatant IL 1beta concentrations in response to hypoxia reoxygenation were studied in the presence or absence of different concentrations of lidocaine ( 0 . 005 , 0 . 05 and 0 . 5 mg mL ( 1 ) ) . ^^^ RESULTS : Exposure to hypoxia reoxygenation increased neutrophil CD11b ( 94 . 33+ / 40 . 65 vs . 34 . 32+ / 6 . 83 mean channel fluorescence ( MCF ) , P = 0 . 02 ) , CD 18 ( 109 . 84+ / 35 . 44 vs . 59 . 05+ / 6 . 71 MCF , P = 0 . 03 ) and endothelial ICAM 1 ( 146 . 62+ / 16 . 78 vs . 47 . 29+ / 9 . 85 MCF , P < 0 . 001 ) expression compared to normoxia . ^^^ CONCLUSIONS : Lidocaine at clinically relevant concentrations decreased neutrophil CD 18 and endothelial ICAM 1 expression but not endothelial IL 1beta concentrations . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
A semi quantitative RT PCR method was used to determine intracellular adhesion molecule 1 ( ICAM 1 ) , vascular cell adhesion molecule 1 ( VCAM 1 ) , lymphocyte function associated antigen ( LFA 1 ) , LFA 3 , CD 40 , CD 40 ligand ( CD40L ) and RANTES ( regulated upon activation , normal T cell expressed and secreted ) gene expression . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Surprisingly , initial binding of gp 120 to DC SIGN did not result in increased adhesion levels of LFA 1 to its ligand ICAM 1 in both immature DC and Raji DC SIGN cells . ^^^ This triggers binding of ligands to LFA 1 that are shared with DC SIGN , such as ICAM 3 , but not of ligands that are not shared with DC SIGN , such as ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The effects of immunoblockade of P selectin , ICAM 1 , and CD 18 on leukocyte and platelet recruitment were evaluated in obese septic animals . ^^^ These enhanced responses were attenuated by blocking P selectin , CD 18 , or ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In bone , ICAM 1 is expressed at the surface of osteoblasts ( Obs ) and its counter receptor , leukocyte function associated antigen 1 ( LFA 1 ; CD11a ) , at the surface of osteoclast ( Oc ) precursors . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
BACKGROUND : Neutrophil mediated tissue injury in acute pancreatitis includes a severe reduction of the functional microcirculation via interaction of adhesion molecules on leukocytes ( MAC 1 ) and endothelium ( ICAM 1 ) . ^^^ The expression of ICAM 1 or MAC 1 was evaluated by flow cytometry . ^^^ RESULTS : Up regulation of MAC 1 and ICAM 1 expression requires the presence of serum . ^^^ The maximal increase of MAC 1 and ICAM 1 expression was found at concentrations of trypsin or elastase characteristic for acute pancreatitis . ^^^ FUT or FOY significantly reduced protease induced expression of MAC 1 and ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We evaluated the inflammatory response and the expression of cellular adhesion molecules ( ICAM 1 , VCAM 1 , CD 18 , LFA 1 and Mac 1 ) in lungs of BALB / c mice infected with Paracoccidioides brasiliensis conidia . ^^^ These findings suggest that during these early stages , up regulation of ICAM 1 , VCAM 1 , CD 18 and Mac 1 expression occurs , participating in the inflammatory process and as such , in the pathogenesis of paracoccidioidomycosis ( PCM ) . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In addition , T cells require a costimulatory signal delivered through contact with other ligands on APC , especially B 7 and ICAM 1 which bind to CD 28 and LFA 1 , respectively , on T cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Pulmonary microvascular permeability , various cytokine levels ( tumor necrosis factor ( TNF ) alpha , interleukin ( IL ) 1beta , and IL 6 ) , mRNA expressions ( cytokine induced neutrophil chemoattractant ( CINC ) , P selectin , CD 11b / CD18 ( Mac 1 ) , and intercellular adhesion molecule 1 ( ICAM 1 ) ) , and activities of nuclear factor ( NF ) kappaB and activator protein ( AP ) 1 were determined in each group . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We have investigated the distribution , activity , and cytoskeletal linkage of the integrin lymphocyte function associated antigen 1 ( LFA 1 ) on human T lymphocytes migrating on endothelial cells and on ligand intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^ However , high affinity , clustered LFA 1 is restricted to a mid cell zone that remains stable over time and over a range of ICAM 1 densities . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We demonstrate in this study that activation of primary human CD 4 ( + ) T lymphocytes increases LFA 1 affinity and avidity states , two events promoting the early events of the HIV 1 replication cycle through interactions between virus embedded host ICAM 1 and LFA 1 clusters . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
AIM : To examine the expression of intercellular adhesion molecule 1 ( ICAM 1 ) and lymphocyte function associated antigen 1 ( LFA 1 ) expression on canals of Hering ( CoH ) and bile ductules associated with the autoimmune process of bile duct destruction in primary biliary cirrhosis ( PBC ) . ^^^ Immunohistochemistry was performed using anti ICAM 1 and anti LFA 1 mouse mAbs . ^^^ In situ hybridization demonstrated ICAM 1 mRNA expression in bile ductules and LFA 1 mRNA in lymphocytes infiltrating the bile ductules . ^^^ Cells with intermediate morphology resembling progenitor cells in CoH were not labeled with ICAM 1 and LFA 1 . ^^^ CONCLUSION : De novo expression of ICAM 1 both on mature cholangiocytes in CoH and epithelial cells in bile ductules in PBC implies that lymphocyte induced destruction through adhesion by ICAM 1 and binding of LFA 1 expressing activated lymphocytes takes place not only in bile ductules but also in the CoH . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
IL 27 rapidly up regulated the expression of ICAM 1 on naive CD4+ T cells , but not on APCs , and blocking Abs against ICAM 1 and LFA 1 inhibited the IL 27 induced Th 1 differentiation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Adhesion molecules LFA 1 and ICAM 1 , 3 GFP as well as MHC class 2 GFP molecules were very rapidly concentrated at the DC contact area . ^^^ Binding of ICAM 3 , and ICAM 1 to a lesser extent , to LFA 1 expressed by mature but not immature DC , induced MHC 2 clustering into the immune synapse . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Engagement of the beta 2 integrin Mac 1 through its adhesion to its ligands , intercellular adhesion molecule 1 ( ICAM 1 ) and fibrinogen , signals survival cues in neutrophils . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Leukocyte cell adhesion molecules ( LFA 1 , Mac 1 and VLA 4 ) mobilization in circulating granulocytes were analysed by flow cytometry and endothelial cell adhesion molecules ( ICAM 1 and VCAM 1 ) were detected by immunohistochemistry on lung sections . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
RESULTS : In actinic prurigo patients , there was a significant increase in the number of CD 3 , CD 4 , CD 8 , CD45RA , CD45RO , and CD45RB lymphocytes and Langerhans cells , as well as in the level of human leukocyte antigen DR ( HLA DR ) expression and cell adhesion molecules lymphocyte functional antigen 1 ( LFA 1 ) , intercellular adhesion molecule 1 ( ICAM 1 ) , and endothelial leukocyte adhesion molecule 1 ( ELAM 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Experiments were repeated in the presence of blocking antibodies against LFA 1 , PECAM 1 , VCAM 1 , and ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In this study , we report the surprising finding that CTLA 4 engagement by soluble antibody or CD 80 potently up regulates lymphocyte function associated antigen 1 ( LFA 1 ) adhesion to intercellular adhesion molecule 1 ( ICAM 1 ) and receptor clustering concurrent with IL 2 inhibition . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We used immunohistochemistry on cryopreserved human conjunctiva with monoclonal antibodies to T lymphocytes ( CD 3 , CD 4 and CD 8 ) , B lymphocytes ( CD 19 ) , macrophages ( CD 14 ) , natural killer cells ( NK , CD 57 ) , intercellular adhesion molecule 1 ( ICAM 1 ) , E selectin , vascular cell adhesion molecule 1 ( VCAM 1 ) , lymphocyte function associated antigen 1 ( LFA 1 ) , very late antigen 4 ( VLA 4 ) , interleukin 2 receptor ( IL2r , CD 25 ) and HLA DR . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Consequently , we compared the efficacy of anti leukocyte function associated antigen 1 ( LFA 1 ) / anti intercellular adhesion molecule 1 ( ICAM 1 ) monoclonal antibody therapy for facilitating cardiac vs islet long term allograft acceptance in mice . ^^^ Monoclonal anti body therapy with anti LFA 1 , anti ICAM 1 , the combination , or control rat immunoglobulin ( Ig ) was administered intraperitoneally on Days 0 to 5 . ^^^ Mixed lymphocyte assays were performed to assess proliferation of CD 4 and CD 8 T cells under conditions of stimulator cell ICAM 1 and / or LFA 1 deficiency . ^^^ Surprisingly , combined anti LFA 1 / anti ICAM 1 therapy abrogated long term islet , but not cardiac , allograft acceptance relative to either monotherapy . ^^^ Mixed lymphocyte reactions demonstrated complete blockade of CD 4 and CD 8 T cell proliferation under conditions of ICAM 1 deficiency alone or in combination with anti LFA 1 therapy . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
ICAM 1 / LFA 1 interactions are known to enhance T cell / APC interactions and to promote T cell activation and cytokine secretion . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Because this chemokine did not influence the strength of naive T cell adhesion to DCs , enhanced LFA 1 affinity for ICAM 1 was not the main mechanism by which CCL 19 increased Ag independent calcium transients . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The expression of the following cell adhesion molecules and their beta 1 and beta 2 integrin ligands was investigated in the liver tissue from 3 patients with non bacillar peliosis using light and electron microscope immunohistochemistry : intercellular adhesion molecule 1 ( ICAM 1 ) , vascular cell adhesion molecule 1 ( VCAM 1 ) , E selectin , platelet endothelial cell adhesion molecule 1 ( PECAM 1 ) , leukocyte function associated antigen 1 ( LFA 1 ) , macrophage antigen 1 ( Mac 1 ) , and very late antigen 4 ( VLA 4 ) . ^^^ These cells were mainly ICAM 1 , LFA 1 , and VLA 4 positive . ^^^ The prevalence of the ICAM 1 / LFA 1 and VCAM 1 / VLA 4 patterns of mononuclear blood cell / sinusoidal cell interactions could support the macrophage induced or lymphocyte induced type of liver injury . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In addition , mAb against intercellular adhesion molecules 1 ( ICAM 1 / CD54 ) or CD 18 ( beta subunit of ICAM 1 counter receptor ) also blocked gammadelta T cell mediated killing of MDA MB 231 cells . ^^^ Ex vivo expanded human gammadelta T cells are thus able to innately recognize and kill human breast cancer cells in a gammadelta TCR dependent manner ; ICAM 1 and CD 18 also appear to be involved in the interactions between sensitive breast cancer cells and cytolytic gammadelta T cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We also investigated the participation of beta 2 integrin chain , l selectin , LFA 1 , ICAM 1 and PECAM 1 adhesion molecules in the BaV induced leukocyte accumulation . ^^^ Anti mouse l selectin , LFA 1 , ICAM 1 , PECAM 1 and beta 2 integrin chain monoclonal antibodies resulted in a reduction of neutrophil accumulation induced by BaV injection compared with isotype matched control injected animals . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
This paper more particularly focuses on the specific inhibition of the binding of leukocyte function associated antigen 1 ( LFA 1 ) to intercellular adhesion molecule ( ICAM 1 ) by statins , independently of the inhibition of HMGCoA reductase . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The ICAM 1 / LFA 1 complex mediates cell cell interaction . ^^^ To analyze ICAM 1 expression in H / RS cells , we examined the expression of ICAM 1 , LFA 1 , CD 30 and CD30L in HL derived cell lines . ^^^ All cell lines expressed ICAM 1 and CD 30 , but not LFA 1 or CD30L . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Monocyte adhesion was inhibited by antibodies to ICAM 1 and its ligand leukocyte function associated molecule ( LFA 1 ) on monocytes . ^^^ Blocking monocyte binding with ICAM 1 and LFA 1 antibodies reduced the rate of apoptosis to that of the infected culture . ^^^ We conclude that HCMV stimulates ST culture expression of ICAM 1 , which binds to LFA 1 on monocytes that release TNFalpha , thereby inducing apoptosis of neighbouring uninfected trophoblasts . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Interestingly , while the activation of fibroblasts is mediated by a soluble factor , a cognate interaction ICAM 1 mediated was demonstrated to be responsible for the modulation of LFA 1 , CD 28 and CD 69 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
IL 3 , IL 5 , and GM CSF could augment the phosphorylation of p 38 MAPK and nucleus translocation of NF kappaB in eosinophils . cDNA expression arrays demonstrated that the gene expression levels of several adhesion molecules including intercellular adhesion molecule 1 ( ICAM 1 ) , alpha 6 , beta 2 integrin ( CD 18 ) , and CD 44 were upregulated by these cytokines . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Early intrahepatic antigen specific retention of nave CD8+ T cells is predominantly ICAM 1 / LFA 1 dependent in mice . ^^^ This process was largely dependent on LFA 1 and ICAM 1 , but was independent of blocking with antibodies against VCAM 1 , alpha 4 integrin , P selectin , VAP 1 , and beta 1 integrin . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Cell to cell contact is required to support progenitor activity , but can be replaced if receptor ligand engagement of the VLA 4 and LFA 1 complexes is provided through the use of recombinant ligands ( fibronectin , ICAM 1 , VCAM 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
LFA 1 ( CD11a / CD18 ) affinity correlates with specific adhesion functions because a shift from low to intermediate affinity supports rolling on ICAM 1 , whereas high affinity is associated with shear resistant leukocyte arrest . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Interactions between virus associated host ICAM 1 and cell surface LFA 1 under a cluster formation seem to be responsible for the preferential HIV 1 infection of the memory cell subset . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Although 1 3 did not affect the direct binding of LFA 1 to ICAM 1 , these compounds markedly inhibited ICAM 1 expression in HL 60 cells in a dose dependent fashion . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To analyze the expression of adhesion molecules , eosinophils from healthy donors were cultured in the presence of interferon gamma and tumor necrosis factor alpha with various concentrations of fexofenadine , incubated with monoclonal antibodies anti ICAM 1 and LFA 1 and then analyzed by flow cytometry . ^^^ In this study , fexofenadine did not cause any significant changes in the expression of LFA 1 but was shown to be able to inhibit the expression of ICAM 1 at concentrations between 10 ( 3 ) and 10 ( 4 ) M . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Production of these proinflammatory cytokines was dependent on ligation of ICAM 1 expressed on BMSC and its receptor LFA 1 on NK cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Taxifolin also prevented Mac 1 and ICAM 1 expression , two key counter receptors involved in firm adhesion / transmigration of leukocytes to the endothelium , which partially accounted for the limited leukocyte infiltration . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Indeed , T cells of mice treated with hCDR 1 showed a TGF beta induced suppression of adhesion to the LFA 1 and CD 44 ligands , hyaluronic acid and ICAM 1 , respectively , induced by stromal cell derived factor 1alpha and PMA . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
High structural similarity to Dl domains of ICAM 1 and ICAM 2 , and common features in amino acid sequence between X 4 and ICAM 1 , suggest X 4 to possess binding activity for the alpha ( L ) integrin 1 domain of LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
ICAM 1 with its counter receptor , the lymphocyte function associated antigen 1 ( LFA 1 ) is known to play a key role in inflammatory processes and in T cell mediated host defense mechanisms . ^^^ LFA 1 positive reactive microglia gathered in areas of intense ICAM 1 expression , and LFA 1 positive leukocytes were identified infiltrating the tissue . ^^^ Double immunostaining for ICAM 1 and LFA 1 revealed aggregates of reactive microglia embedded in areas of diffuse ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The interaction between integrin lymphocyte function associated antigen 1 ( LFA 1 ) and its ligand intercellular adhesion molecule 1 ( ICAM 1 ) is critical in immunological and inflammatory reactions but , like other adhesive interactions , is of low affinity . ^^^ Here , multiple rational design methods were used to engineer ICAM 1 mutants with enhanced affinity for LFA 1 . ^^^ Five amino acid substitutions 1 ) enhance the hydrophobicity and packing of residues surrounding Glu 34 of ICAM 1 , which coordinates to a Mg2+ in the LFA 1 1 domain , and 2 ) alter associations at the edges of the binding interface . ^^^ The affinity of the most improved ICAM 1 mutant for intermediate and high affinity LFA 1 1 domains was increased by 19 fold and 22 fold , respectively , relative to wild type . ^^^ Thus , rational design can be used to engineer novel adhesion molecules with high monomeric affinity ; furthermore , the ICAM 1 mutant holds promise for targeting LFA 1 ICAM 1 interaction for biological studies and therapeutic purposes . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Tissue abundance of Mac 1 and its ligands ICAM 1 , RAGE and JAM C was examined by Western blot and immunoprecipitation . ^^^ Besides ICAM 1 , an upregulation of two novel Mac 1 ligands , RAGE and JAM C were observed , however , with distinct time courses . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
All three molecules antagonized LFA 1 binding to ICAM 1 Immunoglobulin G fusion ( ICAM 1 Ig ) in a competition ELISA , but only compound 3 and sICAM 1 inhibited the binding of a fluorescein labeled analog of compound 3 to LFA 1 . ^^^ Their antagonism of LFA 1 was surmountable by both ICAM 1 Ig and a fluorescein labeled compound 3 analog . ^^^ The competition of both sICAM 1 and compound 3 with ICAM 1 Ig for LFA 1 resulted in equivalent and linear Schild plots with slopes of 1 . 24 and 1 . 26 , respectively . ^^^ In addition , cells transfected with a variant of LFA 1 lacking this 1 domain showed no significant binding of a fluorescein labeled analog of compound 3 or ICAM 1 Ig . ^^^ These results demonstrate that compound 3 inhibits the LFA 1 / ICAM 1 binding interaction in a directly competitive manner by binding to a high affinity site on LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Adhesion blocking Abs for ICAM 1 , VCAM 1 , CD 18 , and the alpha 4 and beta 1 integrins had no effect on HLMC adhesion . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Efalizumab , a targeted inhibitor of T cell interactions , binds to the CD11a subunit of lymphocyte function associated antigen 1 ( LFA 1 ) , thereby preventing LFA 1 binding to intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The binding site for P . falciparum infected erythrocytes on ICAM 1 has been mapped in some detail and is distinct from the site for lymphocyte function associated antigen 1 ( LFA 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
According to previous report , adhesion of CD 8 positive cells and macrophages to glomerular endotherial cells through the lymphocyte function associated antigen 1 ( LFA 1 ) / intercellular adhesion molecule 1 ( ICAM 1 ) pathway is crucial for the initiation and subsequent progression of anti glomerular basement membrane ( anti GBM ) antibody induced glomerulonephritis ( anti GBM nephritis ) in WKY rats . ^^^ In the present study glomerular inflammatory cell infiltration and LFA 1 / ICAM 1 expression were examined in anti GBM nephritis induced in WKY rats with monoclonal anti GBM antibodies of different subclasses : IgG 1 , IgG2a , and IgG2b . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Induction of LFA 1 dependent neutrophil rolling on ICAM 1 by engagement of E selectin . ^^^ Treating mice with antibodies to the beta 2 integrins LFA 1 and Mac 1 showed that LFA 1 was primarily responsible for mediating rolling on ICAM 1 in this model . ^^^ CONCLUSION : The data show that neutrophil rolling on E selectin leads to partial activation of LFA 1 , enabling LFA 1 dependent rolling on ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
ICAM 1 and LFA 1 play critical roles in LPS induced neutrophil recruitment into the alveolar space . ^^^ In many tissues endothelial intercellular adhesion molecule 1 ( ICAM 1 ) interacts with lymphocyte function associated antigen 1 ( LFA 1 ) on neutrophils , and this interaction plays a critical role in neutrophil recruitment . ^^^ Neutrophil recruitment was reduced by 54 % in ICAM 1 null mice and by 45 % in LFA 1 null mice . ^^^ In wild type mice treated with anti ICAM 1 and anti LFA 1 antibodies , there was 51 and 58 % reduction in the neutrophil recruitment , respectively . ^^^ Neither the treatment of ICAM 1 null mice with a function blocking antibody to LFA 1 nor the treatment of LFA 1 null mice with anti ICAM 1 antibody resulted in further reduction in the recruitment compared with untreated ICAM 1 null and LFA 1 null mice . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
This increase was associated with the up regulated expression of activation dependent epitopes of the beta ( 2 ) integrin LFA 1 and was prevented by antibodies that selectively block cell adhesion mediated by ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
METHODS : T cells were stimulated through the leukocyte function associated molecule 1 ( LFA 1 ) receptor by incubating with either intracellular adhesion molecule 1 ( ICAM 1 ) Fc fusion protein or anti LFA 1 immobilized on 8 well chamber slides . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
However , LFA 1 was shed in a ligand binding conformation , since it expressed KIM 127 and 24 activation epitopes and bound to solid phase ICAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Corticotropin releasing hormone induced a significant time and concentration dependent increase of cell adhesion as well as monocytic MAC 1 expression ; endothelial ICAM 1 and VCAM 1 expression was not altered . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Density functional theory and a polarizable continuum model are used to ( 1 ) understand the affinity modulating mechanisms of the interaction between the metal ion dependent adhesion site ( MIDAS ) of a selected integrin , lymphocyte function associated antigen 1 ( LFA 1 ) and a ligand mimetic acetate molecule and to ( 2 ) propose a new , promising family of inhibitors to block the interaction of the integrin with intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The expression of various adhesion molecules ( intercellular adhesion molecule 1 [ ICAM 1 ] , vascular cell adhesion molecule 1 [ VCAM 1 ] , endothelial leukocyte adhesion molecule 1 [ ELAM 1 ] ) , their ligands ( leukocyte function antigen 1 [ LFA 1 ] , very late antigen 4 [ VLA 4 ] , Sialyl LewisX molecule [ SLeX ] ) , and lymphoid activation markers ( major histocompatibility complex [ MHC ] Class 2 , interleukin 2 receptor [ IL 2R ] ) was demonstrated by immunohistochemistry . ^^^ CMV significantly ( P < 0 . 05 ) increased ICAM 1 and VCAM 1 expression and the number of LFA 1 , VLA 4 , and Class 2 positive lymphocytes in the graft . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Leukocyte mediated killing could be blocked by desferoxamine or by antibodies to CD 18 , which prevent attachment of leukocytes to ICAM 1 expressing chondrocytes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
When stimulated with phorbol ester , nonlytic TIL bind purified ICAM 1 equivalently as lytic TIL , suggesting that LFA 1 can be activated if proximal TCR signaling is bypassed . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
By means of immunocytochemical staining and enzyme immunoassay , the expression of adhesion molecules ( ICAM 1 , VCAM 1 , and CD 44 ) and counterparts ( LFA 1 and VLA 4 ) was investigated . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Biopsies from 10 patients with post transplant HCV were examined for expression of adhesion molecules ICAM 1 , VCAM 1 , and ELAM 1 , number of lymphocytes positive for their ligands LFA 1 , VLA 4 , and SLeX , and activation markers MHC class 2 antigens and IL 2 R by immunohistochemistry . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NPC transplantation down regulated the inflammatory brain process at the acute phase of disease , as indicated by a reduction in the number of perivascular infiltrates and of brain CD3+ T cells , an increase in the number and proportion of regulatory T cells and a reduction in the expression of ICAM 1 and LFA 1 in the brain . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The effects of intensive , moderate and downhill treadmill running on human blood lymphocytes expressing the adhesion / activation molecules CD 54 ( ICAM 1 ) , CD 18 ( beta 2 integrin ) and CD 53 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Following T cell activation , a rapid conformational change of LFA 1 to an `` adhesive ' ' state occurs , allowing LFA 1 binding to intracellular cell adhesion molecule type 1 ( ICAM 1 ) expressing targets , such as antigen presenting cells . ^^^ Experimental controls indicated that the T cell bead attachment was LFA 1 and ICAM 1 specific . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Among them , CD 18 and its ligand ICAM 1 are particularly intriguing because CD 18 and its heterodimer binding chains CD11a and CD11b were correspondingly expressed in ex vivo expanded EPCs isolated from rat and murine bone marrows . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Immunohistochemistry , after three infusions , revealed a marked decrease in the expression of TNF alpha , HLA DR , CD 3 , CD 15 , ICAM 1 and LFA 1 positive cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Cell to cell contact through ICAM 1 LFA 1 and TNF alpha synergistically contributes to GM CSF and subsequent cytokine synthesis in DBA / 2 mice induced by 1 , 3 beta D Glucan SCG . ^^^ Cell cell contact involving intercellular adhesion molecule 1 ( ICAM 1 ) and lymphocyte function associated antigen 1 ( LFA 1 ) was an essential step for the induction of GM CSF and IFN gamma by SCG but not for the induction of TNF alpha or IL 12p70 by SCG . ^^^ We concluded that induction of GM CSF production by SCG was mediated through ICAM 1 and LFA 1 interaction , GM CSF subsequently contributed to further cytokine induction by SCG , and reciprocal actions of the cytokines were essential for enhancement of the overall response to SCG in DBA / 2 mice . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
These differences were not due to soluble factors , viral capture differences , or the expression of integrins ICAM 1 , 2 , 3 , or LFA 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Several classes of small molecule antagonists have been developed to block LFA 1 interaction with intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Effect of ICAM 1 and LFA 1 in hyperleukocytic acute myeloid leukaemia . ^^^ However , the interaction of intercellular adhesion molecule 1 ( ICAM 1 ) and lymphocyte function associated antigen 1 ( LFA 1 ) plays an important role in the adhesion and migration of normal leukocytes and AML cells . ^^^ Therefore , effects of ICAM 1 and LFA 1 were studied in hyperleukocytic AML . ^^^ Finally , we determined the ICAM 1 on the surface of HUVECs treated with the supernatant of hyperleukocytic AML blasts and LFA 1 on hyperleukocytic AML blasts by flow cytometry . ^^^ It showed that the ICAM 1 expression on the surface of the HUVECs treated with hyperleukocytic AML blast supernatant for 24 h could be increased , and the expression of LFA 1 on hyperleukocytic AML was also increased significantly . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Eosinophils are principal effector cells of allergic inflammation and their adhesion onto human bronchial epithelial cells is mediated by a CD 18 intracellular adhesion molecule 1 ( ICAM 1 ) dependent interaction . ^^^ Surface expression of CD 18 and ICAM 1 on eosinophils was greatly increased by Der p 1 ; such inductive effect on ICAM 1 was also found to be more prominent on BEAS 2B cells from the co culture than BEAS 2B cells alone . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Activated lymphocyte function associated antigen 1 ( LFA 1 , alphaLbeta 2 integrin ) found on leukocytes facilitates firm adhesion to endothelial cell layers by binding to intercellular adhesion molecule 1 ( ICAM 1 ) , which is up regulated on endothelial cells at sites of inflammation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The dissociation rates were calculated under low force conditions for ICAM 1 interactions with both beta 2 integrins , LFA 1 ( CD11a / CD18 ) and Mac 1 ( CD11b / CD18 ) , together and separately by using functional blocking antibodies on PMNs . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In regard to the characteristics of adhesive molecules , the endothelial cells were blocked by monoclonal antibodies ( mAbs ) to intracellular adhesion molecule 1 ( ICAM 1 ) , vascular cell adhesion molecule 1 ( VCAM 1 ) ; the NK cells were blocked by mAbs to leukocyte function associated antigen ( LFA 1 ) and very late antigen 4 ( VLA 4 ) before co incubation . ^^^ The blocking percentages of mAbs on NK adhesion in the severe pre eclampsia group were 49 + / 4 % to LFA 1 , 61 + / 48 % , 67 + / 39 % to VLA 4 , ICAM 1 , and 68 + / 7 % to VCAM 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Other cell adhesion peptides derived from ICAM 1 and LFA 1 sequences were found to block T cell adhesion to vascular endothelial cells and epithelial cells ; these peptides are being investigated for treating autoimmune diseases . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
OBJECTIVE : Leukocyte diapedesis is misregulated in inflammatory disease and depends on the binding of monocytic LFA 1 and VLA 4 to endothelial ICAM 1 and VCAM 1 , respectively . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Studies showed that LF elevates the number and increases the activity of T and B lymphocytes and NK cells , stimulates the release of a number of cytokines ( IL 1 , 6 , 8 , 18 , IFN gamma , TNF alpha ) , increases phagocytic activity and cytotoxicity of monocytes / macrophages , accelerates the maturation of T and B cells , and elevates the expression of several types of cellular receptors , such as CD 4 , zeta chain of the CD 3 complex , LFA 1 , CD 11 , ICAM 1 , and selectin P . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In autopsy brains of PD , the number of MHC class 2 ( CR3 / 43 ) positive activated microglia , which were also ICAM 1 ( CD 54 ) , LFA 1 ( CD 11a ) , TNF alpha , and IL 6 positive , increased in the SN and putamen during progress of PD . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
A growing body of data strongly supports the in vivo role of lymphocyte function associated antigen 1 ( CD 18 ) expression by T cells in the binding to intercellular adhesion molecule 1 ( CD 54 ) expressing keratinocytes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Besides facilitating cell to cell adhesion , the molecular interactions between CD 2 and its ligand CD 58 ( lymphocyte function associated antigen 3 [ LFA 3 ] ) , as well as between CD11a / 18 ( LFA 1 ) and CD 54 ( intercellular adhesion molecule 1 ) have recently been recognized to participate in lymphocyte activation , recirculation , and effector function , including cytolytic activity towards tumor cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Also , both lymphocyte function associated antigen 1s , CD11a and CD 18 , and intercellular adhesion molecule 1 were demonstrated on the tumor cells and vascular walls in the lesions . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
A competitive ELISA was developed to measure serum concentrations of immunoreactive R6 . 5 , a mouse IgG2a monoclonal antibody which binds to and inhibits the interactions of human intercellular adhesion molecule 1 with glycoproteins of the CD 18 complex and with rhinoviruses . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Although antibodies to intercellular adhesion molecule 1 ( CD 54 ) and the beta chain ( CD 18 ) of lymphocyte function associated Ag 1 ( CD11a / CD18 ) partially inhibited T cell adhesion to unstimulated EC , they did not affect the increase in adhesion due to IL 4 stimulation , indicating that the increased binding resulted from the generation of an alternative binding receptor ( s ) on the EC membrane . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The adhesiveness of C5a stimulated endothelium for neutrophils was blocked by anti P selectin , but not by antibodies to intercellular adhesion molecule 1 , E selectin , or CD 18 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
This adherence was inhibited by mAb to either LFA 1 subunit and by mAb to the counter receptor intercellular adhesion molecule 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Human microglia expressed other costimulatory molecules , such as intercellular adhesion molecule 1 , LFA 1 and LFA 3 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
These results suggest that neutrophil endothelial adhesive interactions , which occur via CD11a / CD 18 and CD11b / CD18 dependent interactions with intercellular adhesion molecule 1 , and oxygen derived free radicals produced by neutrophils are implicated in the production of aspirin induced gastric mucosal injury . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
There were differences in adhesion molecule expression between CNS 5 beta 4+ T cells and peripheral 5 beta 4+ T cells , although both populations were in activated state . 5 beta 4+ T cells at the site of Ag expression ( the spinal cord ) demonstrated higher levels of LFA 1 and CD 44 , but lower levels of VLA 4 and intercellular adhesion molecule 1 , than did 5 beta 4+ T cells in the spleen . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Pretreatment with monoclonal antibodies against the leukocyte adhesion molecule CD 18 or the endothelial cell adhesion molecules intercellular adhesion molecule 1 and P selectin significantly attenuated the I / R induced permeability increase and lung sequestration of neutrophils , mononuclear leukocytes , and eosinophils . ^^^ We conclude that leukocyte adherence in the pulmonary , microvasculature and subsequent permeability increase after I / R is dependent on the integrin CD 18 , its endothelial cell ligand intercellular adhesion molecule 1 , and the endothelial cell rolling factor P selectin but not the leukocyte rolling factor L selectin . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Y 24180 had no effect on the expression of intercellular adhesion molecule 1 or endothelial leukocyte adhesion molecule 1 on endothelial cells activated with interleukin 1 beta , while it suppressed the expression of CD 18 antigen on human peripheral blood polymorphonuclear cells activated with leukotrien B 4 , inhibiting their adhesion to endothelial cells activated with interleukin 1 beta . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In addition , CD 40 mAb induced increased surface expression of intercellular adhesion molecule 1 ( CD 54 ) , a ligand for CD11a / CD18 ( LFA 1 ) , and CD 18 mAb blocked aggregation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Lymphocytes ( CD 2 positive ) that infiltrated the iris were predominantly helper T cells ( 70 % ) and strongly expressed the lymphocyte function associated antigen 1 ( CD11a , CD 18 ) molecule , the ligand for intercellular adhesion molecule 1 , in four of the seven biopsy specimens . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Cytokine mRNA expression was correlated with TNF alpha bioactivity , renal intercellular adhesion molecule 1 expression , and CD 18 positive leukocyte infiltration by immunohistochemistry . ^^^ The infiltrating leukocytes expressed the leukocyte beta 2 integrin ( CD 18 ) and were found in areas with increased intercellular adhesion molecule 1 expression . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Both CD 3 and T cell receptor cross linking have been associated with lymphocyte activation and expression of the LFA 1 adhesion ligand , which leads to adhesion to intercellular adhesion molecule 1 immobilized on artificial surfaces . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
However , sICAM 1 in solution , at concentrations up to 100 micrograms / ml , was incapable of inhibiting the phorbol ester induced homotypic aggregation of lymphocytes , the adhesion of T cells to plastic immobilized sICAM 1 , and CTL effector function , all of which are mediated by intercellular adhesion molecule 1 : LFA 1 interaction . ^^^ These results suggest that although monomeric sICAM 1 can not competitively inhibit cell adhesion mediated by intercellular adhesion molecule 1 and LFA 1 , microspheres coated with sICAM 1 can inhibit such cell adhesion . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
These studies indicate that , in the cobra venom factor model of acute lung injury in rats , engagement of Mac 1 , lymphocyte function associated Ag 1 , and intercellular adhesion molecule 1 are essential , whereas , in contrast to other models of neutrophil mediated lung injury , cytokines ( TNF alpha and IL 1 ) and very late Ag 4 are not required for the full development of injury . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Furthermore , pretreatment with individual monoclonal antibodies against cell adhesion molecules ( CD11a , CD11bc , CD 18 , and intercellular adhesion molecule 1 ) reduced not only the infiltration of leukocytes but also the area of infarction in the reperfused hearts . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
However , as determined by flow cytometry , pentoxifylline did not alter TNF alpha induced upregulation of intercellular adhesion molecule 1 or vascular cellular adhesion molecule 1 on endothelioma cells nor did it affect constitutive CD11a , CD 18 , or alpha 4 integrin expression on T lymphoma cells , suggesting that rather than affecting quantitative expression of these adhesion molecules , pentoxifylline might modulate their avidity . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Hi and lo CD 43 MO expressed comparable amounts of CD 14 , CD 45 , and intercellular adhesion molecule 1 ( CD 54 ) ; hi CD 43 MO expressed somewhat more lymphocyte function associated antigen 1 alpha chain ( CD11a ) and CD 18 than their lo CD 43 MO counterparts . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Some animals were treated with monoclonal antibodies against leukocyte ( CD 18 ) or endothelial cell ( intercellular adhesion molecule 1 , P selectin ) adhesion molecules before radiation and 5 hours thereafter . ^^^ Treatment with antibodies against either CD 18 or intercellular adhesion molecule 1 , but not P selectin , significantly attenuated leukocyte adherence , emigration , and the increase in permeability induced by radiation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Peripheral blood monocytes were cultured for 1 day , treated with various concentrations of immunosuppressants , and subsequently analyzed for expression of macrophage differentiation antigen RM3 / 1 , intercellular adhesion molecule 1 ( CD 54 ) , beta 2 integrin ( CD 18 ) , and HLA DR using flow cytometry and indirect immunofluorescence microscopy . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Consistent with this interpretation , CD 28 8+ T cells were morphologically large , granular lymphocytes and expressed CD 54 ( intercellular adhesion molecule 1 ) , CD 58 ( lymphocyte function associated Ag 3 ( LFA ) ) , and high levels of CD11a ( LFA 1 ) , but did not express Ag associated with acute activation ( e . g . , HLA DR , CD 25 , CD 69 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
This increase in PMN adherence was almost completely blocked by MAb R15 . 7 , a monoclonal antibody against CD 18 of PMNs , and was significantly attenuated by MAb RR1 / 1 , a monoclonal antibody against intercellular adhesion molecule 1 of ECs ( p < 0 . 01 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Furthermore , an antibody against a ligand for CD 18 complexes , intercellular adhesion molecule 1 , also prevented TNF induced shock as well as endotoxin shock in rabbits . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We have investigated whether hapten specific unresponsiveness could be induced if the interaction between LFA 1 ( CD11a / CD18 ) and intercellular adhesion molecule 1 ( CD 54 ) was disrupted by blocking mAbs given to mice during sensitization with 2 , 4 dinitro 1 fluorobenzene . ^^^ The contact sensitivity response was significantly reduced ( p < 0 . 001 ) when a combination of mAb FD441 . 8 against LFA 1 and mAb YNI / 1 . 7 . 4 against intercellular adhesion molecule 1 was given during the sensitization phase compared with normal rat IgG treated control animals . ^^^ Together these data indicate that inhibition of LFA 1 / intercellular adhesion molecule 1 mediated interactions between APCs and T cells during sensitization induced long term , Ag specific , hyporesponsiveness of mice to the hapten 2 , 4 dinitro 1 fluoro benzene . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
These data suggest that ischemia and reperfusion injury of rat lower extremities causes systemic changes that result in neutrophil dependent lung injury that is beta 2 integrin ( leukocyte function antigen 1 , Mac 1 ) and intercellular adhesion molecule 1 dependent . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
It becomes increasingly clear that adhesion systems such as CD2 / LFA 3 ( lymphocyte function associated antigen 3 ) , LFA 1 / ICAM 1 ( intercellular adhesion molecule 1 ) and Thy 1 / putative Thy 1 ligand participate in the association between murine thymocytes and thymic epithelial cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The aspirin induced increase in PMN adherence to HUVEC was significantly reduced by monoclonal antibodies against CD 18 , CD11b , CD11a , and intercellular adhesion molecule 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In this study , the effects of CPB on the expression of neutrophil CD11b and CD 18 ( the components of the Mac 1 adhesion molecule ) were examined ; the effects of membrane versus bubble oxygenators on the expression of neutrophil CD11b and CD 18 were compared ; and the plasma levels of the intercellular adhesion molecule 1 ( cICAM 1 ) , an inducible endothelial adhesion molecule , were measured . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We previously reported that the action as well as the production of TNF requires the adhesion of leukocytes to the endothelium through integrin beta 2 and intercellular adhesion molecule 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We investigated the hypothesis that CD 54 ( intercellular adhesion molecule 1 ) expressed on hepatocytes will support beta 2 integrin ( CD 18 ) dependent adhesion of neutrophils . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Keratinocytes were activated and expressed CD 54 ( intercellular adhesion molecule 1 ) ; perivascular lymphocytes were mostly CD2+ , CD3+ , and CD4+ and exhibited CD11a through CD 18 ( leukocyte function associated antigen 1 ) and often HLA DR and / or CD62L ( leukocyte endothelial cell adhesion molecule 1 ) ; and endothelial cells were activated with a strong expression of CD 54 ( intercellular adhesion molecule 1 ) , CD62E ( endothelial leukocyte adhesion molecule 1 ) , and CD 31 ( platelet endothelial cell adhesion molecule 1 ) in lesser amounts . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To examine the mechanism of inhibition by protein kinase C ( PKC ) inhibitors of the adhesion of highly malignant hepatoma AH66F cells to the mesentery derived mesothelial cell ( M cell ) layer through leukocyte function associated antigen 1 ( LFA 1 ) / intercellular adhesion molecule 1 , the effects of a PKC inhibitor , NA 382 , on the expression of LFA 1 molecules in AH66F cells were examined and compared with those in thymocytes from normal rats . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Furthermore , an antibody to a ligand for CD 18 , intercellular adhesion molecule 1 , also prevented TNF induced shock as well as endotoxin shock in rabbits . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To investigate the molecular basis of such reversibility in human T lymphocytes , we developed a model of alpha L / beta 2 ( LFA 1 ) dependent adhesion that uses a heterologous cell line expressing human intercellular adhesion molecule 1 as a selected ligand . ^^^ Cells costimulated via the CD 3 and LFA 1 receptors by specific Abs or by binding to intercellular adhesion molecule 1 display gradual and persistent intracellular cAMP elevations due to the synergistic induction of a protein kinase C dependent adenylyl cyclase isoform . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Soluble intercellular adhesion molecule 1 provokes polymorphonuclear leukocyte elastase release by CD 18 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Addition of monoclonal antibody directed against either rat CD 18 or intercellular adhesion molecule 1 also attenuated the increased NO production of Kupffer cells and the alterations of AH 70 cells . ^^^ The CD 18 intercellular adhesion molecule 1 dependent adhesive interaction with hepatoma cells triggers NO production by Kupffer cells . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In contrast , chemoattractants stimulated a prolonged increase in the adhesiveness of Mac 1 ( alpha M beta 2 , CD11b / CD18 ) for intercellular adhesion molecule 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
These findings implicate CD11 / CD 18 , L selectin , intercellular adhesion molecule 1 , and P selectin in the leukocyte recruitment elicited by oxLDL and invoke a role for adherent leukocytes in the accompanying increase in mast cell degranulation and albumin leakage . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Using lymphocyte function associated antigen 1 ( LFA 1 ) on T cells as a model , we have investigated adhesion to ligand intercellular adhesion molecule 1 induced by the Ca2+ mobilizers , ionomycin , 2 , 5 di t butylhydroquinone , and thapsigargin , and the well studied stimulators such as phorbol ester and cross linking of the antigen specific T cell receptor ( TCR ) CD 3 complex . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Thus we sought to determine whether endothelial expression of intercellular adhesion molecule 1 ( a ligand for CD 18 ) in pulmonary capillaries mediates neutrophil adhesion in this setting . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The aim of the study was to evaluate the soluble form of intercellular adhesion molecule 1 ( sICAM 1 ) , a ligand of LFA 1 , in the serum of patients with spondylarthropathies ( SpA ) and to look for a correlation with several inflammatory parameters . sICAM 1 levels were measured by ELISA in 25 SpA patients , 20 healthy controls and 20 patients with rheumatoid arthritis ( RA ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Hydrodynamic shear shows distinct roles for LFA 1 and Mac 1 in neutrophil adhesion to intercellular adhesion molecule 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Bronchial epithelial cells express the intercellular adhesion molecule 1 that mediates binding of activated neutrophils via interaction with Mac 1 and / or leukocyte function associated antigen 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Mice deficient in the counterreceptor for intercellular adhesion molecule 1 , the leukocyte integrin alphaMbeta 2 ( Mac 1 ) , showed a similar obesity phenotype . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We observed that LFA 1 expressing K 562 can not bind to intercellular adhesion molecule 1 coated surfaces when stimulated by phorbol 12 myristate 13 acetate ( PMA ) , whereas the LFA 1 activating antibody KIM 185 markedly enhanced adhesion . ^^^ Both deletion of the beta 2 cytoplasmic tail or replacement by the beta 1 cytoplasmic tail alters the localization of LFA 1 into clusters , thereby regulating LFA 1 activation and LFA 1 mediated adhesion to intercellular adhesion molecule 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Platelet aggregation was reduced by antibodies directed against either P selectin , CD 18 , or intercellular adhesion molecule 1 , as well as a GPIIb IIIa antagonist . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The extent of neutrophil migration across IL 1 treated ( 10 U / ml for 4 h ) HUVEC monolayers was the same whether tight junctions were present or absent , and the molecular requirements for neutrophil transmigration ( CD 18 and intercellular adhesion molecule 1 ) were unaffected by culturing in astrocyte conditioned medium . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
PMN activation was measured cytometrically using the following antibodies against PMN surface membrane receptors : L selectin ( CD 62 L ) , beta 2 integrin Mac 1 ( CD 11b ) and Intercellular Adhesion Molecule 1 ( CD 54 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Since it is not fully understood how LFA 1 mediates transmigration , we studied the effects of alpha L and beta 2 cytoplasmic domain mutants that alter LFA 1 adhesiveness for intercellular adhesion molecule 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The number of eosinophils , the level of eosinophil cationic protein ( ECP ) , the bindings of soluble intercellular adhesion molecule 1 ( sICAM 1 ) and fibronectin ( FN ) , and the expressions of CD11b ( alpha chain of Mac 1 ) and CD49d ( alpha chain of VLA 4 ) on eosinophils were evaluated in peripheral blood ( n=4 ) and BALF ( n=3 ) before and after the administration of Y 24180 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We report here that LFA 1 mediated binding of mouse thymocytes or activated T lymphocytes to intercellular adhesion molecule 1 can be rapidly induced by clustering of membrane rafts using antibodies to the glycosylphophatidylinositol anchored molecule CD 24 or cholera toxin ( CTx ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Intercellular adhesion molecule 1 , EB 22 , Mac 1 , and glial fibrillary acidic protein mRNA levels were elevated within the first 3 days of exposure in the absence of increased inducible nitric oxide synthetase and interferon gamma mRNA . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We investigated the expression of adhesion molecules in circulating neutrophils ( lymphocyte function associated antigen 1 [ LFA 1 ] , Mac 1 , and L selectin ) and endothelial cells ( soluble intercellular adhesion molecule 1 [ sICAM 1 ] ) in 23 patients with stable chronic obstructive pulmonary disease ( COPD ) , 18 subjects with exacerbated COPD , and 23 healthy volunteers . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
OBJECTIVE : To analyze the expression and cellular distribution of intercellular adhesion molecule 1 , E selectin ( endothelial leukocyte adhesion molecule ) , vascular cell adhesion molecule 1 , very late antigen 4 , and lymphocyte function associated antigen 1 ( LFA 1 ) in diseased and normal human sclera . ^^^ METHODS : Monoclonal antibodies to vascular cell adhesion molecule 1 , very late antigen 4 , intercellular adhesion molecule 1 , LFA 1 , and E selectin were used to perform immunohistochemical staining on frozen sections of 16 cryopreserved human sclera specimens and 5 conjunctival specimens . ^^^ Intercellular adhesion molecule 1 , the ligand for LFA 1 , was expressed in 7 of 12 scleral specimens . ^^^ Furthermore , the expression of LFA 1 and intercellular adhesion molecule 1 were focally present in areas of inflammatory infiltrate . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Auranofin ( AF ) interacted with HUVEC and PMN adhesiveness but in opposite directions : this drug hampered IL 1beta induced HUVEC hyperadhesiveness and expression of E selectin and intercellular adhesion molecule 1 , but augmented PMN adherence and CD 18 expression . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We show that CC chemokines induced a sustained increase in monocyte adhesion to intercellular adhesion molecule 1 that was mediated by Mac 1 ( alphaMbeta 2 ) but not lymphocyte function associated antigen 1 ( LFA 1 ; alphaLbeta 2 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We report here that lovastatin , a drug clinically used for lowering cholesterol levels , inhibits the interaction of human LFA 1 with its counter receptor intercellular adhesion molecule 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Immunosuppressive potentials of the blockade of intercellular adhesion molecule 1 ( ICAM ) 1 / leukocyte function associated antigen 1 ( LFA 1 ) were examined in a murine islet allotransplantation model by using blocking monoclonal antibodies ( MAbs ) against these molecules . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Although lymphocyte function associated antigen 1 ( LFA 1 ) is critical for entry into lymph nodes , we show here that in the absence of functional LFA 1 molecules , lymphocytes can still enter the white pulp , in spite of the high expression of intercellular adhesion molecule 1 on sinus lining cells in the marginal zone . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Inhibition of Rap 1 either by Rap GTPase activating protein ( RapGAP ) or the Rap 1 binding domain of RalGDS abolished both Mn ( 2+ ) and KIM 185 ( anti LFA 1 ) induced LFA 1 mediated cell adhesion to intercellular adhesion molecule 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Binding to iC3b and factor 10 by 1 less Mac 1 was inhibited by the function blocking antibody CBRM1 / 32 , which binds to the beta propeller domain of the alpha ( M ) subunit . 1 less LFA 1 did not bind its ligands intercellular adhesion molecule 1 and 3 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Systemic IL 3 vaccine treatment increased intratumoral levels of intercellular adhesion molecule 1 , Mac 1 , EB22 / 5 . 3 , tumor necrosis factor alpha , and IL 1 mRNA in irradiated tumors , indicating that cellular infiltration was part of the response . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Moreover , the antibody based neutralization of intercellular adhesion molecule 1 and CD 18 is shown to prevent both leukocyte adhesion and retinal endothelial cell injury and death . ^^^ They also underscore the potential utility of anti intercellular adhesion molecule 1 and anti CD 18 based therapies in the treatment of diabetic retinopathy , a newly recognized inflammatory disease . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Blockade of alpha ( 4 ) beta ( 1 ) integrin ( VLA 4 ) or vascular cell adhesion molecule 1 , but not CD 18 or intercellular adhesion molecule 1 , almost completely inhibited the arrest of monocytes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
METHODS : By using the erythrocyte deformability apparatus , platelet and thrombus adhesion dual purpose apparatus and enzyme linked immunosorbent assay ( ELISA ) , We measured the leukocyte deformability ( LD ) , leukocyte adhesion function ( LAF ) , leukocyte CD 18 expression , soluble intercellular adhesion molecule 1 ( sICAM 1 ) and soluble vascular cell adhesion molecule ( sVCAM 1 ) concentration in 36 MOF patients , 31 trauma patients , and 35 to be controls . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Peripheral blood was collected at the day prior to surgery and the morning of postoperative days 1 , 2 , 5 , and 7 for determination of the expression of leukocyte CD11a , CD 18 , CD11a / CD18 , CD11b , CD11b / CD18 ( immunofluorescent flow cytometry ) and the plasma soluble intercellular adhesion molecule 1 ( sICAM 1 ) , soluble E selectin ( sE selectin ) ( enzyme linked immunoabsorbent assay ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Second , chemokine induced lymphocyte function associated antigen 1 ( LFA 1 ) activation was assayed by measuring the adhesion of cells to microculture plates coated with purified intercellular adhesion molecule 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The LFA 1 ligand , intercellular adhesion molecule 1 is critical for lymphocyte entry and both hematopoietic cells and radiation resistant cells contribute to this requirement . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In a cross sectional study , we investigated platelet leukocyte aggregate formation and markers indicative for platelet , leukocyte , and endothelial activation ( CD 62 , activated fibrinogin receptor glycoprotein IIb / IIIA [ PAC 1 ] , CD11b / CD18 [ MAC 1 ] , and soluble intercellular adhesion molecule 1 ) in 44 patients with atherosclerotic vascular disease and peripheral occlusions receiving clopidogrel ( n = 12 ) , aspirin ( n = 17 ) , their combination ( n = 8 ) , or no treatment ( n = 7 ) , as well as in a group of healthy subjects ( n = 9 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Moreover , induction of IP 10 was mediated via specific adhesion molecules , as indicated by the finding that both anti integrin ( CD11b and CD 18 ) and intercellular adhesion molecule 1 antibodies significantly inhibited IP 10 induction . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Infected dimethyl sulfoxide differentiated HL 60 cells and neutrophils showed reduced binding to cultured brain and systemic endothelial cells and lost expression of P selectin glycoprotein ligand 1 ( PSGL 1 , CD 162 ) and L selectin ( CD62L ) ( to 33 and 5 % of control values , respectively ) , at a time when the levels of beta ( 2 ) integrin and immunoglobulin superfamily adhesion molecules and activation markers Mac 1 and intercellular adhesion molecule 1 increased ( 5 to 10 times that of the control ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Soluble intercellular adhesion molecule 1 and soluble vascular cell adhesion molecule 1 were measured by enzyme linked immunosorbent assay and expression of the monocyte integrins CD11b ( Mac 1 ) and CD49d ( VLA 4 ) was measured by direct immunofluorescence using flow cytometry . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Up regulation of CD 54 and the subsequent apoptosis of B CLL cells depend on the initial LFA 1 / ICAM 1 ( intercellular adhesion molecule 1 ) interaction . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Also , PRL promotes the adhesion of PBMC to immobilized intercellular adhesion molecule 1 and fibronectin , ligands for LFA 1 and VLA 4 , respectively . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To support lymphocyte adhesion , this extended LFA 1 conformation required immediate activation by its ligand , intercellular adhesion molecule 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
LFA 1 mediated adhesion to glass supported bilayers containing intercellular adhesion molecule 1 was characterized by a much larger junction area , marked by LFA 1 segregation , and a more compact cell shape compared with those observed for CD 2 mediated adhesion to bilayers containing CD 58 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Co crystals of several integrin ligand complexes have been published , including alpha 5 beta 3 with ligand fragments , alpha IIb beta 3 with multiple therapeutic ligands and alpha L beta 2 ( leukocyte function associated antigen 1 [ LFA 1 ] ) with its cell based ligand intercellular adhesion molecule 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Lymphocytes from Lfa 1 ( d / d ) mutant mice showed constitutive activation of LFA 1 mediated cell adhesion and impaired de adhesion from intercellular adhesion molecule 1 that resulted in defective cell migration . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
This study concerns the role of the adhesive molecules CD 18 and CD 54 for the LTB 4 ( as well as thrombin and PAF ) induced endothelial hyperadhesiveness . ^^^ The MoAbs 60 . 3 ( to the CD 18 molecule on PMN ) and 84H10 ( to one epitope of CD 54 on the HUVEC ) blocked the adherence of PMN to LTB 4 treated HUVEC , whereas MoAb LB 2 ( directed at another CD 54 epitope ) failed to do so . ^^^ Thus , LTB 4 induced HUVEC hyperadhesiveness may therefore be related to a specific domain on the CD 54 ( or on an antigenically related molecule ) as well as being dependent on CD 18 , whereas the involvement of CD 54 was much less or non existent for the thrombin and PAF responses , respectively . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In addition , the presence at the membrane of some cellular adhesion molecules ( CD 54 , CD 58 , CD 29 , CD 18 , CD 56 ) was examined in anthracyclin treated cells and in the four clones . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
These nonphagocytic cells appear to be related to follicular dendritic cell ( FDC ) as they do not have properties of monocytic lineage cells or dendritic cells and because , like FDC , 1 ) they express CD11b , CD 14 , CD 29 , CD 40 , CD 54 , CD 73 , CD 74 , and VCAM 1 , and do not express CD11c , CD 22 , T cell markers , CD 18 , CD 25 and CD 45 ; and 2 ) they bind human B lymphocytes and B cell lines , but not T lymphocytes by an adhesion blocked in part by mAb to VLA 4 ( CD49d ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Large cell lymphomas often involve the stomach ; commonly express the lymphocyte adhesion molecules CD 44 , LFA 1 ( CD11a and CD 18 ) , and CD 54 ; and may express monotypic cytoplasmic immunoglobulin in approximately one third of cases . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Among the mAb that inhibited B cell DNA synthesis and / or Ig production , only mAb to CD11a , CD 18 , or CD 54 inhibited initial B cell activation as assessed by RNA synthesis . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Antibody to CD 18 exerted the most significant inhibition of adhesion and cytotoxicity of endogenous NK cells to MOLT 4 lymphoma cells , followed by antibodies to CD 2 and CD 54 . ^^^ Antibodies against CD 18 , CD 54 and CD 2 exerted an additive effect on the inhibition of adhesion . ^^^ The RGD peptide substantially inhibited the binding of IL 2 stimulated killer cells to COLO , and the combination of this peptide with MAbs to CD 18 , CD 54 and CD 2 practically blocked the adhesion . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The following molecules were detected in patients before culture : CD 18 ( mean percentage 94 % ) , CD11a ( 94 % ) , CD11b ( 39 % ) , CD 54 ( 23 % ) , CD 58 ( 62 % ) , CD 44 ( Hermes 1 ; 82 % ) , CD 49 d ( VLA 4 ; 80 % ) , CD 25 ( 11 % ) and LECAM 1 ( 62 % ) . ^^^ After stimulation with PHA , there was an increase in the levels of CD 18 ( 2 . 5 fold ) , CD11a ( 2 . 3 fold ) , CD 54 ( 10 . 2 fold ) , CD 58 ( 2 . 5 fold ) , CD 44 ( 2 . 4 fold ) , CD49d ( 3 . 4 fold ) and CD 25 ( 62 fold ) on lymphocytes from both patients and controls . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies ( MoAbs ) against CD11a , CD11b , CD 18 , and CD 54 and a polyclonal anti human fibronectin antibody do not inhibit attachment of either B or T lymphoblastic cells to MSC . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Homotypic adhesion could be inhibited by mAb to CD11b , CD 18 and CD 54 , indicating an involvement of the beta 2 integrin cyto adhesion pathway . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Treatment with Abs against CD11a , CD 18 , or CD 54 inhibited not only the homotypic adhesion but also the inhibition of anti IgM Ab induced apoptosis by anti CD 40 Ab . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
CD 29 and CD49d were detected on cells from day 0 to 4 weeks of culture ; CD49b , CD49c , CD49f , CD 18 , and CD 54 expression was negligible . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
With regard to surface marker profile , the established cells were positive for CD11a , CD 13 , CD 18 , CD 33 , CD 34 , CD41b , CD42b , CD 54 , and c kit antigens , but negative for HLA class 2 antigen and glycophorin . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Electroassociation of glycophorin A did not cause a decrease in the expression of either `` positive signals ' ' for NK cells ( such as CD 71 , CD 15 , and CD 32 antigens ) or cellular adhesion molecules ( CD 18 , CD 29 , CD 54 , and CD 58 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In comparison with unstimulated cell lines , incubation of the three lines with various amounts of IL 2 over 3 and 14 days did not increase their growth or change message expression of IL 2R , IL 10 , and TGF beta and surface expression of the adhesion molecules CD 11 , CD 18 , CD 29 and CD 54 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Ia , CD 54 , CD11a and CD 18 ) were not affected . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
On day 6 , most T cells coexpressed the CD11a , CD 18 , CD 54 , CD45R0 antigens and consisted of activated ( CD25+ ) CD4+ and CD8+ cells in nearly equal proportions . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Analysis of the expression of adhesion receptors on the canine peripheral lymph DC revealed the presence of CD 54 , CD 58 , CD 18 as well as CD49d and CD49e molecules and cell surface fibronectin . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The level of expression of CD 9 , but not that of CD11a , CD 18 , CD 19 , CD 44 , or CD 54 , was found to correlate with the level of aggregation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Epstein Barr virus ( EBV ) negative and EBV carrying Burkitt lymphoma ( BL ) lines that remain phenotypically similar to the in vivo tumor cells ( operationally defined group 1 BLs ) express high levels of CD 10 and CD 77 , and lack immunoblastic markers such as CD 23 and CD 39 , and the cell adhesion molecules CD11a , CD 18 , CD 54 and CD 58 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Immunohistochemistry showed that BDC were strongly positive for antibodies against HLA DR , CD11c , CD 18 , CD 44 and CD 54 , and moderately positive for anti CD11a , CD 31 , CD 43 and CD 58 . ^^^ Preincubation of HUVEC with antibodies against E selectin ( 10 micrograms / ml ) significantly inhibited ( P < 0 . 02 ) the binding of radiolabelled BDC to activated HUVEC ( 32 . 2 + / 1 . 3 % ) whereas preincubation of BDC with antibodies against CD 54 , CD 18 , CD11b , CD11c and Sialyl Lewis 10 did not produce any significant inhibition . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Of note , anti CD 14 mAb decreased the expression of CD 54 , but not that of CD11a or CD 18 , on monocytes , suggesting that inhibition of the soluble anti CD 3 induced IFN gamma production by anti CD 14 mAb might be a result from decrease in CD11a / CD18 CD 54 mediated interactions between CD4+ T cells and monocytes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In CDC stage B / C patients , whose monocytes ' ability to phagocytose bacteria and migrate across confluent endothelial monolayers was diminished , surface expression of CD49e and CD62L and the percentage of monocytes expressing CD 18 , CD11a , CD 29 , CD49e , CD 54 , CD 58 , CD 31 , and HLA 1 were significantly decreased . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The expression of adhesion molecules in vitro was differential : the number of cells carrying CD11c , CD 29 , CDw49b , CDw49d , CDw49e , CDw49f , CD 54 , CD 56 and ELAM 1 being significantly overrepresented among the nickel induced lymphoblasts whereas the number of blasts carrying CD 44 was underrepresented and those of CD11a , CD 18 , CD 58 and LAM 1 remained unchanged . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
IL 8 selectively induced expression of HLA DR antigen , but had no effect on the expression of other cell surface antigens ( CD11a , CD 18 , CD 36 and CD 54 ) . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
MoAb staining and flow cytometric analysis were used to follow changes in surface expression of LeuCAMs and the LFA 1 ligand CD 54 during maturation of peripheral blood monocytes ( BM ) in vitro . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Surface expression of the beta 2 integrin chains ( CD11a , CD11b , CD11c and CD 18 ) , and the beta 1 integrin chains ( CD49b , CD49d , CD49f and CD 29 ) , as well as that of members of the immunoglobulin superfamily ( CD 2 , CD 54 , CD 56 and CD 58 ) , were analyzed by one or two color flow cytometry . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The mAbs to CD 18 and CD 54 completely inhibited the LPS U 937 cell aggregation induced by mNI 11 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In 68 patients with different leukemias the expression of the following adhesion molecules was examined : CD11a , CD 18 , CD 54 , CD 44 , CD 58 , and CD 59 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
U 266 and IM 9 myeloma cell lines express mainly CD 29 , CD49d , VLA 1 , CD 18 , CD 54 , ICAM 2 and ICAM 3 . ^^^ The combination of these MoAbs ( anti CD 29 , CD 18 , CD11a , CD49d , VLA 5 , CD 44 , CD 54 , ICAM 2 , ICAM 3 MoAbs ) decreased but did not completely abrogate binding of IM 9 to BMSCs . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Surface expression of the beta 2 integrin chains ( CD 18 , CD11a , CD11b , CD11c ) , as well as that of members of the immunoglobulin superfamily ( CD 2 , CD 54 , CD 56 , CD 58 ) , were analysed by one or two colour flow cytometry . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Six or 7 days after RLV infection DC expressed lower levels of Iad but not H2Dd molecules in parallel with lower expression of some adhesion molecules ( CD 18 , CD 54 , CD 44 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Adhesion of PBMC to synoviocytes was increased by IL 1 beta or TNF alpha and reduced by MAb to CD 54 or CD 18 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Furthermore , mAb to CD 2 , CD 3 , CD 8 , Class 1 , Class 2 , CD 54 and LFA 1 did not block TC mediated perforin mRNA down regulation although lysis of TC was inhibited . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
This reversal of the IFN alpha mediated suppression of B cell function was not blocked by any of the mAb to CD11a , CD 18 , CD 54 , or monomorphic determinants of HLA DR . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We investigated 49 acquired immunodeficiency syndrome related lymphomas ( ARLs ) for Epstein Barr virus ( EBV ) by Southern blotting and in situ hybridization and , in positive cases , used cryostat immunohistology to compare EBV latent gene expression ( EBV encoded small RNA 1 [ EBER 1 ] , EBV nuclear antigen 2 [ EBNA 2 ] , latent membrane protein 1 [ LMP 1 ] and host cell immunophenotype ( CD11a , CD 18 , CD 54 , CD 58 , CD 21 , CD 23 , CD 30 , CD 39 , CDw 70 , immunoglobulin ) patterns with those reported in other EBV infections . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Except for CD11b , all other leukocyte integrins examined ( CD11a , CD11c and CD 18 ) and their ligand ( CD 54 ) were highly expressed in non CLL cases . ^^^ Adhesion activity of leukemic cells to HUVECs after co culture with HUVECs was well correlated with the expression of CD11b , CD 18 and CD 54 , but showed no predilection for any leukemia subtype . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
METHOD : In this study , PBM and PM from fertile women ( Group 1 ) , women with unexplained infertility ( Group 2 ) , and women with limited ( Group 3 ) or severe ( Group 4 ) endometriosis were isolated in order to analyze these cells for the expression of CD 54 , CD 58 and HLA DR ( immunoglobulin supergene antigens ) CD 18 and CD 29 ( integrins ) and CD 44 ( an addresin ) . ^^^ RESULTS : No differences were detected in the percentage of PBM expressing CD 18 , CD 44 , CD 54 , CD 58 , or HLA DR among the four groups of subjects . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
This binding was increased when platelets were activated by thrombin and was decreased by an anti CD11a , CD 18 and anti CD 54 mAb . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Analysis of expression of other adhesion molecules demonstrated that CD11a , CD 18 , CD 44 , CD 45 , CD 48 , CD 54 , and CD62L were significantly increased by either anti CD 2 or anti CD 3 mAbs while the combination was not synergistic . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We examined the expression of adhesion molecules of the integrin family ( CDw49b , CDw49d , CDw49e , CDw49f , CD 18 , CD11a CD11c , and CD11b ) , the immunoglobulin superfamily ( CD 54 , CD 56 , CD 58 , and CD 31 ) , the selectin family ( ELAM 1 , LECAM 1 , and CD 62 ) , and CD 44 , CD41b , and CD42b on platelets , megakaryocytes , and megakaryocytes with CP . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In a retrospective study based on 107 B CLL patients , the expression of the adhesion molecules CD 44 , CD11a , CD11b , CD11c , CD 18 , CD 25 and CD 54 was analysed in bone marrow cryostat sections by immunohistochemistry . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
To examine the effect of cuprophane hemodialysis on expression of cell surface proteins involved in leukocyte adhesiveness , we monitored CD11b , CD 18 , CD 14 , CD 54 , and plasma soluble CD 54 in 10 patients during hemodialysis with cuprophan dialyzers . ^^^ Leukocyte expression of CD11b and CD 18 increased and CD 14 decreased with hemodialysis , while CD 54 remained unaltered . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The increased adhesion of granulocytes to hsp 65 stimulated EC was inhibited completely by blocking Ab against CD62E . mAb against endothelial CD62E , CD 106 , or CD 54 or against the monocyte adhesion molecules CD 14 or CD49d ( very late Ag 4 ) did not inhibit the increased adhesion of monocytes to hsp 65 stimulated EC ; however , mAb against the monocyte adhesion molecule CD 18 ( beta 2 integrin ) inhibited monocyte adhesion to hsp 65 stimulated EC to the same extent as monocyte adhesion to nonstimulated EC . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Incubation of CLC with monoclonal antibodies against HLA DR , CD11b , CD 18 , CD 50 , CD 54 , CD 58 or CD 80 , but not anti major histocompatibility complex class 1 ( MHC 1 ) , inhibited antigen specific T cell proliferation to varying degrees . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Furthermore , SA 1042 does not interfere with the binding of monoclonal antibodies directed to other molecules , gp 41 , CD 4 , CD11a , CD 18 , CD 26 , CD 44 or CD 54 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Expression of other integrin , selectin , or immunoglobulin superfamily molecules ( CD11a , CD 18 , CD 44 , CD 45 , CD 48 , CD 54 and CD62L ) were all significantly increased by anti CD 2 or anti CD 3 mAbs . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
When the reactive patterns between mNI 58A and the mAbs to various human differentiation antigens ( CD11a , CD11b , CD11c , CD 14 , CD 16 , CD 18 , CD 23 , CD 28 , CD 29 , CD 31 , CD 43 , CD 44 , CD45RA , CD 50 , CD 54 , CD 58 , CD 80 , CD 102 , CD 106 , HLA class 1 and class 2 antigen ) were compared , that of mNI 58A was found to be similar to those of the leukocyte function associated antigen 1 ( LFA 1 ) mAbs . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
DLGL are CD56bright and express the CD 2 , CD11a , CD 18 , CD 38 and CD 50 molecules , dimly the CD 54 molecule , and poorly the CD 102 and CD 69 molecules . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In this study , we investigated the role of adhesion molecules , such as CD 2 , CD11a , CD 18 , and CD 54 in the recruitment of pit cells to the liver . ^^^ Immunostaining for electron microscopy , and two color flow cytometry showed that most pit cells expressed CD 2 , CD11a , CD 18 , and CD 54 . ^^^ These results indicate that blocking of CD 2 , CD11a , CD 18 , and CD 54 antigens on blood LGL and / or liver endothelium decreased the number of pit cells in the liver . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
No changes in HLA DR or in other adhesion protein ( CD11a , CD11c , CD 18 , CD 54 ) expression were detected , and levels were similar in both patients and normal controls . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Moreover , CD11a and CD 18 were among two adhesion molecules which showed a sharp increase in expression , whereas no changes on expression of CD11b , CD 44 and CD 54 were detected . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In conclusion , CD 54 expression and low CD 18 expression are both significantly associated with poor prognostic features . . ^^^ Expression of surface adhesion molecules of the integrin family ( CD11a / CD18 or LFA 1 , CD11c / CD18 ) , of the immunoglobulin related family ( CD 54 ) , of the selectin family ( CD62L or LAM 1 ) and the lymphocyte homing receptor ( CD 44 ) were analyzed in peripheral cells from 113 B CLL patients . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Efforts now are directed to development of monoclonal antibodies ( MAB ) to receptors of T cells , B lymphocytes , MAB to CD 18 and CD 54 , cytokines , search for natural inhibitors of cytokines , introduction of cytokines . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
OBJECTIVE : The purpose of this study was to examine the effects of physical exercise on the expression of clusters of differentiation ( CD ) 11b , CD 18 and CD 54 on granulocytes and complement 3bi ( C3bi ) dependent functions in rats . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Nucleosomes impaired neither the conjugation step nor the expression of adhesion molecules at the effector ( CD11a , CD 18 , CD 2 ) or target ( CD 54 , CD 58 ) cell surface . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Analyzing the effect of Pro alpha 1 on IL 2 activated lymphocytes by flow cytometry revealed an increase of CD 16 , CD 56 , and CD 18 surface marker expression , whereas CD 3 , CD11a / b , CD49d , and CD 54 were not affected . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Cells were analyzed from the arterial line before , at 5 , 10 , 15 , 30 , 90 and 240 min with a panel of 33 surface markers including antibodies towards CD 3 , CD 4 , TCR alpha / beta , CD 7 , CD 8 , CD11a c , CD 14 , CD 18 , CD 19 , CD 25 , CD 28 , CD 29 , CD 38 , CD 44 , CD49a e , CD51 / 61 , CD 54 , CD 56 , CD 58 , CD 62 , CD 106 , and HLA class 2 using single fluorescence analysis . ^^^ Our results suggest a substantial difference in the expression of various surface markers and cell adhesion molecules ( lymphocytes : CD 4 , CD 25 , CD 28 , CD 51 , CD 54 ; monocytes : CD 49 , CD 54 , CD 58 , CD62E , CD62P ) compared to healthy persons , and a distinct modulation during hemodialysis ( lymphocytes : CD 11 , CD 18 , CD 49 , CD 51 , CD 61 ; monocytes : CD 29 , CD 49 , CD 51 , CD 54 , CD 61 ) which might affect the immune response beyond the single dialysis session . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
A significantly lower expression of CD 18 and CD 54 as well as a decrease in CD 44 expression level were observed at the surface of infected monocytes when compared with mock infected cultures . ^^^ Except for CD 18 , the expression of which at the cell surface is decreased , no modification of CD 44 and CD 54 expression was observed after heat inactivated HIV 1 treatment of monocytes . ^^^ In addition , pretreatment of infected monocytes with a TNF alpha synthesis inhibitor , RP 55778 , or with MAbs directed against IL 1beta , confirmed the role of TNF alpha and IL 1beta in the regulation of CD 18 , CD 44 , and CD 54 expression . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The homotypic cell aggregation was blocked by another anti CD 53 mAb , MEM 53 , in all the examined cell lines and by anti LFA 1 ( CD11a / CD18 ) or anti ICAM 1 ( CD 54 ) mAbs in the cell lines except for the LAD line , but it was not blocked by anti CD 44 or anti CD49d mAb . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The expression of CD 18 , CD49d / CD29 , CD 44 , CD 54 and CD 106 was studied in the testis of normal mice at various ages , in the cryptorchid testis , in the testis of estrogen treated mice and in the testis of non obese diabetic ( NOD ) mice , using immunocytochemistry to see which of these lymphocyte and endothelial adhesion proteins may be involved in lymphocyte regulation in the testis . ^^^ CD 18 , CD49d / CD29 , CD 44 and CD 54 expressing cells were not found in the normal > 10 week old BALB / c mouse testis . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The phenotype of intraepithelial and lamina propria lymphocytes was assessed by FACS analysis using markers for CD 3 , TCR alpha beta , TCR gamma delta , CD 4 , CD 8 alpha , CD 8 beta , CD 5 , CD 18 , CD 54 , and CD49d . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
It could be reduced with monoclonal antibodies ( MoAbs ) against CD11a and CD 18 on IFN gamma or TNF alpha activated HUVEC but was generally enhanced after treatment of HUVEC with MoAbs against CD 54 , CD62E , or CD 106 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Feeding the FO diet significantly reduced both the proportion of CD 2 positive L DC and the level of CD 2 expression on L DC compared with feeding each of the other diets ; the proportions of L DC staining positive for CD 40 , CD 18 , CD 54 , CD11a , and MHC 2 were unaffected by diet . ^^^ However , FO feeding reduced the level of expression of CD 18 , CD11a , MHC 2 , and CD 54 on L DC compared with feeding the other two diets ; the level of expression of CD 40 was unaffected by diet . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Inclusion of an inhibitor of protein glycosylation and exocytosis ( brefeldin A ) at all stages of separation partially prevented an increase in the percentage of DCs bearing CD 18 , C 29 and C 54 whereas the inclusion of cycloheximide ( an inhibitor of polypeptide synthesis ) interfered with increases in the percentage of cells bearing CD 29 and CD 54 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The levels of expression of HLA class 1 molecules , LFA 1 ( CD11a / CD18 ) , CD 54 and CD 58 were also studied and found to be expressed very heterogeneously . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In this paper we describe the age dependent development of components involved in major maturational processes , including the appearance and varying expression of adhesion receptors ( CD11a , CD 18 , CD 28 , CD 29 , CD 44 , CD49d and CD 54 ) on CD4+ `` helper ' ' cells and CD8+ `` suppressor and cytotoxic ' ' cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The expression of CAM was analyzed by flow cytometry using monoclonal antibodies against CD49d , CD 29 , CD11a , CD11b , CD11c , CD 18 , CD62L , CD 54 and CD 44 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
MATERIAL AND METHODS : Using immunofluorescence phenotyping and flow cytometry , the expression of leukocyte adhesion molecules CD 54 , CD11a , CD11b and CD 18 in peripheral blood were measured within 12 h after onset of ischemia in 20 patients with stroke . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
IEL and LPL were isolated from the small intestine and the phenotype was assessed by FACS analyses , using antibodies for detection of T cell markers CD 3 , TCR alpha beta , TCR gamma delta , CD 4 , CD 8 alpha , CD 8 beta , CD 5 , CD 18 , CD 54 , and CD49d . ^^^ The adhesion molecules CD 18 , CD 54 , and CD49d , essential for cellular communication of lymphocytes , showed an expression peak at weaning . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Altered expression of adhesion molecules CD 44 , CD 54 ( case 1 ) and CD11a and CD 18 ( case 2 ) may help to explain the poor outcome of these patients . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
CBDC expressed the CD 4 , CD11a , CD 18 , CD45RA , CD 50 and CD 54 antigens but revealed no expression of the CD1a , CD11c , CD 40 , CD45R0 , CD 58 , CD 83 , CD 86 and CD 102 antigens . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We studied by simple and double immunolabeling techniques on tissue sections of normal human skin the phenotype of these cells and found them to express vimentin and Te 7 but none of the remaining markers sought ( factor XIIIa , von Willebrand factor , CD1a , CD 3 , CD 4 , CD 8 , CD 14 , CD 25 , CD 36 , CD 45 , CD 54 , CD 56 , LFA 1 , EGF R , S 100 protein , Mac 387 , and muscle specific actin ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We studied the expression adhesion molecules CD11a , CD 18 , CD 44 , CD 54 and CD62L on the thoracic duct lymphocytes of rats treated with CsA . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Glomerular volume , sclerosis in glomeruli ( segmental and global ) and interstitium ( semiquantitative scale ) , infiltrating lymphocytes and macrophages ( CD 43 and ED 1 positive cells ) , and expression of adhesion molecules ( CD 54 , CD 18 , and CD11b ) were analyzed . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Despite the high level of cellular activation and proliferation induced by PMVEC coculture , the surface expression of adhesion molecules CD11a ( LFA 1 ) , CD11b , CD15s ( sialyl Lewis 10 ) , CD 43 , and CD 44 ( HCAM ) on the total CD34+ population was maintained , and the surface expression of CD49d ( VLA 4 ) , CD 54 ( ICAM ) , CD 58 , and CD62L ( L selectin ) increased after ex vivo expansion . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We therefore studied the expression of CD 54 and its ligands CD 18 and CD11b / c in CSS in 10 rats with CSS , 6 rats immunized similarly who did not developed proteinuria ( no CSS group ) , and 10 normal rats ( control group ) . ^^^ The CSS group over expressed CD 54 in glomeruli and interstitium in association with increments in CD 18 and CD11b / c positive cells ranging 2 . 5 to 7 times the number found in controls . 75 % of infiltrating leukocytes expressed CD 18 and 87 % expressed CD11b / c . ^^^ We concluded that over expression of CD 54 and its ligands CD 18 and CD11b / c in infiltrating leukocytes occur in CSS . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
CBDC expressed CD 4 , CD11a , CD 18 , CD45RA , CD 50 , CD 54 , and CD 123 , but showed no expression of CD1a , CD11c , CD 33 , CD 40 , CD45R0 , CD 80 , CD 83 , and CD 86 and only limited expression of CD 58 , CD 102 , and CD 116 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The CD1a+ cells reacted with antibodies against protein S 100 , HLA DR , the Lag antigen , CD 4 , CD11a , CD11b , CD 18 , CD 25 , CD 40 , CD 54 , CD 80 and CD 86 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Cells expressing CD 4 , CD 8 , CD 18 , CD49d / CD29 , CD 44 , CD 54 , CD 80 , CD 86 , CD 106 , CD11b / CD18 or DNA breaks were stained in the pancreases of female or male scid / scid mice after adoptive transfer of lymphocytes from older than 12 week female nonobese diabetic ( NOD ) or Balb / c mice . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We have also found a significant increased expression of CD11a , CD 18 and CD 54 antigens on granulocytes , and higher percentage CD11b+CD18 ( + ) granulocytes out of all granulocytes in MS patients compared with control . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We have studied the immune response of the host testis against experimental testicular teratoma in mouse by localizing adhesion molecules ( CD 106 , CD 54 , CD49d / CD29 , CD 44 , CD 18 , CD 8 and CD 4 ) , cytokines ( IL 2 , IL 4 , IL 6 , IL 10 and IL 12 ) , T cell costimulators ( CD 80 , CD 86 ) and the lipid antigen presenting molecule CD1d in the testis of 129 / SvJ mice with and without experimental testicular teratoma . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies ( mAb ) to the known accessory molecules CD11a , CD 18 , CD 54 and CD 45 , all inhibited T cell proliferation . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies against CD 54 , CD11a , and CD 18 significantly inhibited the cytolysis , indicating that the killing involves the engagement of CD 54 with its ligands . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The ProTalpha effect on PBMC was demonstrated to involve stimulation of adhesion molecules ( CD 2 , CD 18 , CD 54 , CD49f ) and CD 25 expression , up regulation of perforin mRNA transcription , increased numbers of perforin positive ( + ) cells and elevated production of interleukin 2 ( IL 2 ) , interleukin 1beta ( IL 1beta ) and tumor necrosis factor alpha ( TNFalpha ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
RESULTS : Flow cytometric analysis showed that pit cells expressed CD 2 , CD11a , CD 18 and CD 54 . ^^^ The combination of mAbs to CD11a , CD 18 and CD 54 further increased the inhibition of pit cell mediated CC531s cytolysis and apoptosis . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Flow cytometry was used to determine PMN receptor expression ( CD11b , CD 14 , CD 16 , CD 18 , CD 54 ) , phagocytosis , and apoptosis . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Using flow cytometry , we found an increased expression of adhesion molecules CD62L , CD 18 , CD11a , CD11b , CD49d and CD 54 in the exposed workers as compared with the control group ( P < 0 . 05 ) . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The blood was incubated with neutralizing monoclonal antibodies to CD 18 , CD11a , CD11b , CD 29 , CD49d , CD 54 , alpha4beta7 , or isotype matched control antibodies , respectively , at 4 degrees C for 30 min . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
METHODS : Whole blood and SF cells were stained with monoclonal antibodies against CD11a ( LFA 1 ) , CD 15 s ( sialyl Lewis 10 ) , CD 44 , CD 54 , VLA 4 , and HLA DR counterstained with anti CD 14 antibodies as a Mphi marker for dual fluorescence analysis by flowcytometry . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The studies revealed that the integrins CD11a , CD11b , CD 18 , CD49d , and CD 61 , as well as the additional adhesion receptors CD 54 , CD62L , and the transferrin receptor CD 71 were expressed on day 11 of gestation to various degrees on these embryonic cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Extracellular release of free fatty acids by rat T lymphocytes is stimulus dependent and is affected by dietary lipid manipulation . [ ( 3 ) H ] Arachidonic acid labelled rat T lymphocytes released radioactivity extracellularly when stimulated by the calcium ionophore A 23187 or by monoclonal antibodies to some cell surface structures ( CD 2 , CD 5 , CD11a , CD 18 , CD 54 , T cell receptor ) but not to others ( CD49d , CD62L ) ; release was greater with the calcium ionophore . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The antigens recognized by R 1 73 , R 2 40 and R 1 34 mAbs were defined by immunoprecipitation and Western blot analyses as CD11a , CD 18 and CD11b , respectively ; and the allografts expressed CD 54 , a ligand of CD11a or CD11b , suggesting leukocyte integrin dependent killing . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
CI resulted in an increase in CD 18 cells and an increase in the expression of CD62P on vessels , and CD 54 and CD 44 on tubules . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
These markers included CD 2 , CD11b , CD11c , CD 18 , CD 16 , CD 54 , CD 56 , CD 98 , CD 25 , CD 122 , HLA class 1 , HLA class 2 , CD 94 , ILT 2 , p58 . 1 , p 70 , and NKp 46 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Eighteen metastatic nodes derived from the wild type ( GR 9 ) and from 4 different clones ( G 2 , D 8 , B 10 , and B 9 ) obtained from a fibrosarcoma were analyzed for H 2 class 1 and 2 expression , as well as for adhesion molecules ( CD 44 , CDIIb , CD 18 , CD 49 , and CD 54 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In addition , we analysed ( by flow cytometry ) whether this function correlated with modifications of the CD 2 , CD11a , CD11b and CD 18 molecules , which are involved in the adhesion of effector cells to the counter receptors ( CD 54 and CD 58 ) on osteosarcomas . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In contrast , the expressions of Toll like receptor 4 , and CD 18 , CD 54 , CD 59 , and HLA A , B , C on monocytes were slightly increased and decreased , respectively , by 0 . 3 microM HRGP and KGP . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The lipopolysaccharide ( LPS ) mediated stimulatory effects of tumor necrosis factor alpha ( TNF alpha ) cytokine production and cell adhesion molecule ( CD11c , CD 18 , CD 14 , CD 54 ) expression in human monocytic THP 1 cells were modulated by treatments of the TW extracts or tacrolimus ( FK 506 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Additionally , the appearance of several activation ( CD 25 , CD 69 , HLA DR ) and adhesion ( CD11a , CD11b , CD11c , CD 18 , CD49b , CD49d , CD 54 ) receptors on CD 4 cells were analyzed . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In addition , the surface markers alpha 2 , alpha 3 , alpha 4 , CD11a , CD11b , CD 18 , CD 54 , and CD 8 found on lymphocytes and alpha 3 , alpha 4 , CD11a , CD 18 , and CD 8 surface markers found on granulocytes were also decreased in the patient population . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Monoclonal antibodies against CD11a , CD 18 and CD 54 , but not CD11b , restored eosinophil suppression of T lymphocyte proliferation which was chymostatin resistant in coculture . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
An optimal antigen presentation requires the expression of MHC class 2 HLA DR on monocytes surface and of co stimulatory molecules such as CD 54 on monocytes and LFA 1 on lymphocytes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Immunostaining techniques were used to follow CSS and to study the expression of CD 54 , CD 18 , CD11b / c , IFN gamma , TNF alpha and MHC class molecules . ^^^ The renal expression of CD 54 and its ligands ( CD 18 and CD11b / c ) was reduced by 50 % to 75 % . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
METHODS : MFI expression and percentage of CD 18 , CD11b , CD62L and CD 54 were evaluated resting and activated by 30 min . , fMLP preincubation using monoclonal antibodies anti CD11b and anti CD62L , employing Flow Cytometer FACSCalibur with computer program CELLquest . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We now extend our observations in 71 DLCL patients by analyzing malignant B cell expression of the following molecules important in T cell activation : ( a ) recognition molecules [ MHC 1 ( MAS and MCA ) and MHC 2 ( HLA DR , DP , DQ ) ] ; ( b ) CAMs [ leukocyte function antigen 1 ( CD11a and CD 18 ) and intracellular adhesion molecule 1 ( CD 54 ) ] ; and ( c ) CSMs [ B7 . 1 ( CD 80 ) and B7 . 2 ( CD 86 ) ] . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Cell surface markers ( CD 3 , CD 4 , CD 8 ) and adhesion molecules ( CD 18 , CD 54 , CD 2 ) were analyzed using specific monoclonal antibodies ( MoAbs ) and flow cytometry on the PBMC , freshly isolated and treated for 72 hours with medium alone , PHA , r alpha 2b IFN and their combination . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
All expressed high levels of CD11a ( LFA 1 ) , CD11b ( Mac 1 ) , and CD 54 antigens , with low levels of class 2 major histocompatibility complex . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We analyzed the phenotype of CB monocytes in the presence or absence of an inflammatory signal ( rIFN gamma ) and compared them to adult blood ( AB ) ; the expression of HLA DR and 17 different markers ( CD11a , CD11b , CD11c , CD 18 , CD 29 , CD 40 , CD 44 , CD49a , CD49d , CD49e , CD49f , CD 54 , CD 58 , CD62L , CD 80 , CD 86 and CD 102 ) was measured by flow cytometry . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
At 14 weeks of culture , more mature CHMCs expressed CD11b , CD11c , CD 29 , CD49b , CD49c , CD49d , CD49e , CD 51 , CD 61 , and CD 54 and weakly expressed CD 18 and CD11a . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
An optimal antigen presentation requires the expression of MHC class 2 HLA DR on monocytes surface and of costimulatory molecules such as CD 54 on monocytes and LFA 1 on lymphocytes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
On the other hand , CD11a , CD 18 , CD 31 , CD49d , or CD 54 was not markedly enhanced on the U 937 cells by culture with cytochalasin E , although the mean fluorescence intensities ( MFIs ) of CD11a , CD 18 , and CD 54 on U 937 was partially up regulated . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The expression of CD 18 , CD44s , CD 54 , CD62L were investigated with flow cytometry by using monoclonal antibodies . ^^^ Two year overall survival was 83 and 29 % in CD 18 ( + ) and CD 18 ( ) cases ; 55 and 36 % in CD44s ( + ) and CD44s ( ) cases ; 46 and 42 % in CD 54 ( + ) and CD 54 ( ) cases ; 42 and 50 % in CD62L ( + ) and CD62L ( ) cases . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In fact , depending on the environment ( organ ) , stage of cell maturation , type of disease , and other factors , MC express variable amounts of activation linked antigens ( CD 25 , CD 63 , CD 69 , CD 88 ) , cell recognition molecules ( CD 2 , CD 11 , CD 18 , CD 50 , CD 54 ) , or cytokine receptors . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We explored the role of CD 18 ( beta 2 integrin ) in platelet physiology , using mice genetically deficient in CD 18 ( CD 18 / ) , or its main ligand CD 54 ( ICAM 1 , CD 54 / ) . ^^^ The life span of platelets was significantly shorter in CD 18 / than in + / + or CD 54 / mice , as seen by in vivo biotinylation . ^^^ The content of Bcl 3 was about 20 fold higher in platelet from CD 18 / , than in those from + / + or CD 54 / donors , as seen on Western blots or by immunofluorescence and flow cytometry , while the amount of pro caspase 3 was decreased . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
We assessed the effect of CD 54 on eosinophils and neutrophils in recombinant granulocyte macrophage colony stimulating factor ( rGM CSF ) or phorbol myristate acetate ( PMA ) induced superoxide production through CD 18 . ^^^ The increase in CD 18 and CD 54 expression on neutrophils caused by rGM CSF stimulation was partially inhibited by theophylline . ^^^ These data demonstrated that CD 54 and CD 18 interaction of eosinophils or neutrophils is involved in superoxide production and that the inhibition of superoxide production by theophylline may be at least partly due to the inhibition of CD 54 and CD18 . . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Nevertheless , due to a quenching problem when using CD 20 and CD 54 together , and because CD 18 showed a statistically different expression between classical and atypical CLL , the combination of CD18 / CD54 has been preferred and showed a different pattern in the three entities . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
RESULTS : The results show that incubation of CU membranes with the proteins CD11b , CD 18 , and CD 54 or the blockade of these cell surface molecules with specific monoclonal antibodies inhibited the CU induced aggregation and apoptosis in a dose dependent manner . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
They expressed CD 4 , CD11a , CD11b , CD11c , CD 18 , CD 32 , CD 33 , CD45R0 , CD 48 , CD 50 , CD 54 , CD 58 , CD 64 , CD 86 , CD 102 , CD 116 , CD 123 , and HLA DR ; showed no expression of CD1a and CD 83 ; and weak expression of CD 16 , CD45RA , and CD 80 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Comparing to control group adhesion molecules CD11b , CD 18 , CD 31 , CD49d , CD 54 and CD62L were increased on the surface of patient neutrophils whereas no differences were observed in the expression of CD11a abd CD 15 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
This study showed that presentation of SEA by RPE cells to resting T cells was dependent on the presence of the molecules CD 2 , CD 58 and CD 18 , CD 54 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Using an array of function blocking monoclonal antibodies ( mAb ) ( anti CD11a , CD11b , CD 18 , CD49d , CD 54 , and CD 106 ) , we characterized the adhesive interactions underlying the transendothelial and transepithelial leukocyte traffic in intact animals . ^^^ Comigrating neutrophils were found to primarily utilize CD11b , CD 18 , and CD 54 , but not CD49d , CD 106 , or , surprisingly , CD11a . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
OBJECTIVE : To explore the protein expression level of the lymphocyte function related antigen CD11a , CD 18 , CD 54 of breast cancer patients ' blood , cancer tissue and drainage lymph node , and the effect of chemotherapy as well as Yiqi Yangxue Granule on its expression level . ^^^ METHODS : The immunohistochemical assay and flow cytometry were used to determine the change of the protein expression level of CD11a , CD 18 , CD 54 on the lymphocyte of blood , cancer tissue and lymph node between before and after chemotherapy and chemotherapy plus Yiqi Yangxue Granule in 72 patients suffering from breast cancer . ^^^ RESULTS : In breast cancer patients , the level of CD 54 was increased more obviously than that of control group , the CD11a , CD 18 , CD 54 of lymphocytes in cancer tissue increased remarkably , while the CD 54 expression positive rate reached 100 % , the expression level of CD11a , CD 18 , CD 54 which increased significantly in negative lymph node obviously higher than that of positive lymph node . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The CD11b , CD 18 , and CD 54 receptors are implicated in CD 34 ( + ) cell adhesion to BM microenvironment . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Compared to healthy controls , there was decreased expression of CD11a , CD11b , CD 18 , CD49b , CD49c , CD49d , CD 54 , and CD 8 in patients ' lymphocyte populations and CD11a , CD 18 , CD49c , CD49d , and CD 8 in patients ' granulocyte populations . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Antibodies to CD 31 , CD 54 , CD62E , and CD62P on EC or CD11a , CD 18 , and CD 162 on NK cells had only minor effects on rolling adhesion . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The adhesion molecule expression pattern was consistent in all patients : L Selectin and CD11c were negative , CD11alpha and CD 18 weakly positive and CD 54 strongly positive . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In the cryptorchid cryptepididymis CD 4 , CD 8 , CD 18 , CD 44 , CD 54 and CD 106 expressing cells were occasionally present in the connective tissue surrounding the epididymal tubule . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
The importance of cell surface molecules was examined by application of a panel of blocking antibodies ( CD 2 , CD 18 , CD 40 , CD40L , CD 54 , CD 58 ) in addition to use of TCR negative T cell lines . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Resveratrol ( 10 microM ) enhanced the expression of adhesion molecules ( CD11a , CD11b , CD 18 , CD 54 ) in each of the cell lines except for Kasumi 1 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Fluorescence activated cell sorting analyses showed that neither tumors nor ascitic cells grown in SCID mice expressed leukocyte function associated antigen ( LFA ) 1alpha ( CD11a ) , LFA 1lbeta ( CD 18 ) , LFA 2 ( CD 2 ) , LFA 3 ( CD 58 ) , intercellular adhesion molecule ( ICAM ) 1 ( CD 54 ) , ICAM 2 ( CD 102 ) , ICAM 3 ( CD 50 ) , or leukocyte endothelial adhesion molecule ( LECAM ) 1 ( CD62L ) , suggesting that these cytoadhesion molecules are not involved in tumor formation of RM P 1 cells in vivo . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
No significant changes could be found in the cell surface expression of CD 80 , CD 86 , MHC 1 , MHC 2 , CD 18 , CD11b , CD 40 , CD 25 , and CD 54 in antigen presenting cells ( APC ) from control or DSG treated animals . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Cells phenotypes were identified using three color flow cytometry analysis for the following receptors HLA DR , CD 80 , CD 83 , CD 86 , CD11c , CD 18 , CD 54 , CD 58 , CD 3 , CD 4 , CD 8 , CD 19 , CD 20 , CD 14 , CD 16 , CD 56 . ^^^ HLA DR molecules , co stimulatory receptors CD 80 , CD 86 , CD 83 and adhesion molecules CD 18 , CD11c , CD 54 , CD 58 , were analyzed by two color immunofluorescence microscopy on ST serial sections . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
METHODS : In this comparative study , serum immunoglobulins , IgG subclasses , secretory IgA , serum complement components , lymphocyte subsets and CD11a , CD 18 , CD 54 molecules on lymphocytes were determined in 44 hospitalized patients of chronic HBV infection and 20 cases of healthy control subjects . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
When adherence was disrupted by pipetting , by a combination of Abs specific for CD11b , CD 18 , and CD 54 , or by an antisense oligonucleotide targeting CD 18 mRNA , both cell apoptosis and bacterial killing induced by IFN gamma were inhibited . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Likewise , none of the neutralizing mAbs against CD11a , CD 18 , CD 44 , CD49e , or CD 54 showed significant effects . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Cell surface expression of CD 18 , CD11a , CD11b , CD11c , CD 50 , and CD 54 was not affected by Na ( 2 ) SO ( 3 ) treatment . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
ICAM ( CD 54 ) is a ligand for the cell adhesion receptor LFA 1 , shown to be important in immune stimulation that is upgraded in inflammatory cutaneous disorders . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
METHODS AND RESULTS : Nicotine dose dependently ( 10 ( 8 ) to 10 ( 4 ) mol / L ) induced DC expression of costimulatory molecules ( ie , CD 86 , CD 40 ) , MHC class 2 , and adhesion molecules ( ie , LFA 1 , CD 54 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
After 6 weeks of treatment , peripheral blood mononuclear cells ( PBMCs ) expressed lower amounts of CD 54 , CD 18 , and CD11a mRNA compared with pretreatment values . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Immunofluorescence reactions were carried out on cells , to study the distribution of activation ( HLA DR , CD 25 , CD 69 ) and adhesion ( CD11a , CD 18 , CD 54 ) molecules ; the flow cytometry technique was used to analyse cells . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
METHODS : Trace cytotoxicity test plates were utilized in the detection of CD 3 , CD 4 , CD 8 , CD11a , CD11b , CD 18 and CD 54 on lymphocytes by APAAP immunocytochemical staining . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Analysis with Kruskal Wallis test showed no statistical significant differences in the expression of CD 54 , CD11a , CD11b and CD 18 in three groups examined . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Following an additional 3 days of culture in the presence of GM CSF , IL 4 , IL 1beta , IL 6 , TNFalpha , and PGE ( 2 ) , cells ( 82 . 0 + / 5 . 8 % CD83+ ) displayed a mature DC morphology and phenotype , including expression of CD11b , CD11c , CD 18 , CD 25 , CD 40 , CD 54 , CD 58 , CD 80 , CD 86 , HLA class 1 , and HLA DR as well as expression of CCR 7 but not CCR 5 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Most of the DCs express immunocytochemically detectable antigens like : S 100 , CD1a , CD 40 receptor , adhesion molecules ( ICAM 1 or CD 54 , LFA 1 and LFA 3 ) , integrins ( CD11a , CD11c and CD 18 ) , CD 45 , CD 54 , co stimulatory molecules ( B 7 1 or CD 80 , B 7 2 or CD 86 ) , F 418 , MHC class 1 and 2 and DEC 205 , multilectin receptor , immunostimulatory cytokine ( IL 12 ) and , of course , Fc and complement receptors . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Expression of adhesion receptors CD11a , CD11b , CD 18 , CD49d , and CD 54 on blood and peritoneal leukocytes was measured using flow cytometry . ^^^ RESULTS : In macrophages from the uninfected effluents , expression of both subunits of Mac 1 integrin receptor ( CD11b and CD 18 ) and intercellular adhesion molecule ( ICAM ) 1 receptor ( CD 54 ) was upregulated compared to peripheral blood monocytes from the same patients . ^^^ The median value of mean fluorescence intensity in blood and effluent was 760 . 3 versus 1085 . 8 for CD11b ( p = 0 . 013 ) , 288 . 8 versus 448 . 6 for CD 18 ( p = 0 . 003 ) , and 186 . 1 versus 365 . 7 for CD 54 ( p = 0 . 001 ) . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
IL 15 significantly increased neutrophil cell surface expression of CD11b and CD 18 and up regulated A 549 cell surface expression of CD 54 . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
In healthy subjects , particle exposure with exercise reduced expression of adhesion molecules CD 54 and CD 18 on monocytes and CD 18 and CD49d on granulocytes . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
Altered expression of CD 18 and CD 54 , respectively , in blood and peritoneal lymphocytes was also noted . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
TRL 01 had the same immunophenotype as the original leukemia cells : positive for CD 13 , CD 33 , CD11a , CD 18 , CD 29 , CD49d , CD49e , CD 54 , CD62L , and CD 117 , and negative for CD 3 , CD 4 , CD 8 , CD 19 , CD 34 , CD41a , CD41b , CD 135 , and myeloperoxidase . ^^^
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA
Interacting proteins: P05362 and P05107	Pubmed	SVM Score :0.0
NA