Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P08514 and P05106	Pubmed	SVM Score :1.091391
The subunits of the endothelial VnR ( VnR alpha and GPIIIa ) have been studied for their ability to express alloantigens associated with platelet GPIIb and IIIa . 1.091391^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.55574404
To determine how this mutation impaired GPIIb IIIa expression , recombinant GPIIb containing the mutation was coexpressed with GPIIIa in COS 1 cells . 0.55574404^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.52535482
These observations suggest that a domain encompassing 324E of GPIIb is essential for heterodimerization with GPIIIa and its substitution for a positively charged residue precludes normal subunit association . . 0.52535482^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
The non covalent and Ca ( 2+ ) dependent heterodimer GPIIb / IIIa , formed by platelet glycoproteins IIb ( GPIIb ) and IIIa ( GPIIIa ) , also known as the integrin alpha IIb beta 3 , is the inducible receptor for fibrinogen and other adhesive proteins on the surface of activated platelets . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Ser 752 > Pro mutation in the cytoplasmic domain of integrin beta 3 subunit and defective activation of platelet integrin alpha IIb beta 3 ( glycoprotein IIb IIIa ) in a variant of Glanzmann thrombasthenia . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
To better understand the molecular structure of the PlA alloantigen system , located on GPIIIa , and the Bak alloantigen on GPIIb , we used a heterologous mammalian expression system to express these integrin subunits in their known polymorphic forms . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Integrin alpha IIb beta 3 or glycoprotein IIb / IIIa ( GPIIb / IIIa , 228 kDa ) is a Ca ( 2+ ) dependent , noncovalent heterodimer of glycoproteins IIb ( GPIIb or alpha IIb , 136 kDa ) and IIIa ( GPIIIa or beta 3 , 92 kDa ) , which serves as the receptor for fibrinogen and other adhesive proteins at the surface of activated platelets . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Topography of ligand induced binding sites , including a novel cation sensitive epitope ( AP 5 ) at the amino terminus , of the human integrin beta 3 subunit . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Northern blot analysis showed that integrin beta 3 mRNA levels were significantly increased in the HEL cells overexpressing the HOX4A gene , whereas the integrin beta 1 and alpha IIb mRNA levels did not show a distinct correlation with HOX4A mRNA levels . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
A point mutation in the integrin beta 3 cytoplasmic domain ( S 752 > P ) impairs bidirectional signaling through alpha IIb beta 3 ( platelet glycoprotein IIb IIIa ) . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Third , polyclonal antibodies against the GPIIb IIIa complex ( edu 3 and 5B12 ) , human antiserums against platelet alloantigens ( anti Baka / B and anti PLA1 / 2 ) , anti integrin subunits ( anti alpha 5 and anti beta 3 ) , and a wide panel of monoclonal antibodies ( mAbs ) against well known epitopes of GPIIb ( M 3 , M 4 , M 5 , M 6 , and M 95 2b ) and GPIIIa ( P 23 7 , P 33 , P 37 , P 40 , and P 97 ) did not affect platelet LDL binding . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Expression of the mutated integrin beta 3 subunit cDNA in Chinese hamster ovary cells showed that the cDNA gene was transcribed into a full length beta 3 protein with an apparent molecular weight identical to wild type beta 3 and accumulated as a single chain molecule in the cell cytoplasm . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Sequence analysis of PCR amplified genomic DNA and platelet mRNA revealed a C2268T nucleotide substitution in the gene encoding the integrin beta 3 subunit that resulted in an Arg724Ter mutation , producing a truncated protein containing only the first eight of the 47 amino acids normally present in the cytoplasmic domain . ^^^ These studies establish the importance of the membrane distal portion of the integrin beta 3 cytoplasmic domain in bidirectional transmembrane signaling in human platelets , and the role of integrin signaling in maintaining normal hemostasis in vivo . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Phosphorylation sites in the integrin beta 3 cytoplasmic domain in intact platelets . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
A naturally occurring cDNA encoding the Pl ( A 2 ) alloantigen form ( Pro ( 33 ) ) of the integrin beta 3 subunit was subcloned into this construct ( 889Pl ( A 2 ) beta 3 ) and transduced into cells that endogenously synthesized Pl ( A 1 ) beta 3 ( Leu ( 33 ) ) as a marker for detection of provirus derived beta 3 . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Thirdly , polyclonal antibodies against the GPIIb IIIa complex ( edu 3 and 5B12 ) , human antiserums against platelet alloantigens ( anti Bak ( a / B ) and anti PL ( A1 / 2 ) ) , anti integrin subunits ( anti alpha ( 5 ) and anti beta ( 3 ) ) , and a wide panel of monoclonal antibodies ( mAbs ) against well known epitopes of GPIIb ( M 3 , M 4 , M 5 , M 6 , M 8 and M 95 2b ) and GPIIIa ( P 23 7 , P 33 , P 37 , P 40 , and P 97 ) did not affect the binding of HDL ( 3 ) particles to human intact resting platelets . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Both isolated subdomains bound specifically to the integrin beta 3 tail . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Disruption of the long range GPIIIa Cys ( 5 ) Cys ( 435 ) disulfide bond results in the production of constitutively active GPIIb IIIa ( alpha ( IIb ) beta ( 3 ) ) integrin complexes . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Enhanced activation of mitogen activated protein kinase and myosin light chain kinase by the Pro 33 polymorphism of integrin beta 3 . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Critical roles for the COOH terminal NITY and RGT sequences of the integrin beta 3 cytoplasmic domain in inside out and outside in signaling . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
The spatial and temporal association / interaction of DAB 2 and platelet integrin alpha ( IIb ) beta ( 3 ) ( CD 61 ) in both megakaryocytic cells and platelets led us to examine the effect of Ser ( 24 ) phosphorylation on the interaction between DAB 2 and integrin beta ( 3 ) . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Integrin beta 3 was expressed in the grey area . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Southern Blotting was used to analyze the GPIIb and GPIIIa genes in the 11 patients . ^^^ The results showed that there were no major deletions or insertions in either the GPIIb or GPIIIa genes . ^^^ However , a small change in GPIIb gene was demonstrated in two sibling patients and the abnormality of GPIIIa gene was found in another two patients . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
These characteristics are shared by a previously reported defective GPIIb molecule , which is neither assembled with GPIIIa nor transported to the cellular surface . ^^^ Because the precursor GPIIb molecule is assembled with GPIIIa in the endoplasmic reticulum ( ER ) and its processing , as well as stability , is dependent on the GPIIIa subunit , the defective GPIIb molecule may be rapidly degraded by the intrinsic quality control system of the ER due to its inability to form a stable heterodimer complex as a consequence of its misfolded structure . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
To study the relationship between the immunological changes and syndrome ( Zheng , ) groups by TCM of ITP , the T lymphocyte subsets , B lymphocyte , NK cell , platelet associated IgG ( PAIgG , PAIgA , PAIgM ) and antiplatelet autoantibodies ( GPIIb , GPIIIa , GP 1 b ) of 66 patients with ITP were assisted using APAAP and ELISA method separately . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Activated platelets were detected by using a panel of fluoresceinated mAbs specific for activation dependent platelet epitopes , including 1 ) activated GPIIb IIIa complex ( PAC 1 ) ; 2 ) fibrinogen bound to platelet GPIIb IIIa ( 9F9 ) ; 3 ) ligand induced binding sites on GPIIIa ( anti LIBS 1 ) ; and 4 ) P selectin , an alpha granule membrane protein expressed on the platelet surface after secretion ( S 12 ) . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
These results suggest that some interaction of GPIIb / IIIa and linked structures like the platelet cytoskeleton may be involved in the reassociation of dissociated GPIIb and GPIIIa , perhaps explaining the failure of reassociation of microparticle GPIIb / IIIa ( i . e . , the fibrinogen binding to microparticles ) . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Thrombasthenic platelets are deficient in GPIIb , GPIIIa and GPIIb / IIIa complex ; however , platelets from few thrombasthenic patients content near normal amounts of functionally abnormal complex . ^^^ Molecular abnormalities include : GPIIb or GPIIIa partial gene deletion , GPIIIa gene insertion , a point mutation resulting in an amino acid substitution within the GPIIIa molecule . ^^^ In spite of the contemporary reduction of both GPIIb and GPIIIa in most cases of Glanzmann ' s thrombasthenia , it appears that a molecular abnormality affecting only one of the glycoprotein genes may result in a thrombasthenic phenotype . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
The authors describe a method to diagnose thrombasthenic patients and identify carrier subjects by using monoclonal antibodies specific for GPIIb and GPIIIa in an immunoblotting technique . ^^^ The immunoreaction patterns of two thrombasthenic patients lacking GPIIb or GPIIIa , respectively , are shown . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
It has been demonstrated that a molecular defect affecting one of the two GP coding genes is sufficient to determine a contemporary deficit of both GPIIb and GPIIIa , and hence the thrombasthenic phenotype . ^^^ Large rearrangements within the GPIIb or GPIIIa coding genes appear to be unusual , whereas small modifications in the nucleotide sequence of the coding regions occur with higher frequency . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
The short segments of cDNA encoding glycoprotein ( GP ) Ib alpha , GPIIb , GPIIIa and platelet factor ( PF ) 4 were amplified using reverse transcriptase polymerase chain reaction ( RT PCR ) in order to characterize various types of megakaryoblasts . ^^^ GPIb alpha , GPIIb and GPIIIa mRNAs were found to be present in K 562 , CMK and HEL cells , while only HEL cells expressed PF 4 or mRNA . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
GPIIb was cleaved to a 58 kDa NH 2 terminal fragment , whereas GPIIIa remained essentially intact . ^^^ These data suggest that : 1 ) purified active and inactive GPIIb IIIa exist in different conformations and have different affinities for RGDV ; 2 ) certain peptidomimetic inhibitors ( Ro 42 1499 and Ro 43 5054 ) alter the conformation of inactive GPIIb IIIa ; 3 ) GPIIIa and a 58 kDa NH 2 terminal fragment of GPIIb alpha form a high affinity fibrinogen binding complex . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Human megakaryocyte GPIIb and GPIIIa cDNAs were used to create a GPIIb mutant coding for the extracellular GPIIb heavy chain alone ( GPIIb delta 1 ) and a GPIIIa mutant lacking the transmembrane and cytoplasmic domains ( GPIIIa delta m ) . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Immunoblotting with subunit specific polyclonal antibodies showed that the 55 and 85 kDa fragments were derived from GPIIb and GPIIIa , respectively . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Localization of GPIIb and GPIIIa sequences putatively involved in the subunit interface and in intrasubunit and intrachain contacts . ^^^ Human platelet glycoproteins IIb ( GPIIb ) and IIIa ( GPIIIa ) form the subunits of the Ca ( 2+ ) dependent heterodimer GPIIb / IIIa , which belongs to the integrin family of phylogenetically related receptors mediating a wide variety of cell cell and cell substratum interactions . ^^^ Three compact domains have been well defined : one in the heavy ( H ) chain of GPIIb [ GPIIbH ( 600 700 ) ] , and two in GPIIIa , the N terminal [ GPIIIa ( 1 52 ) ] and the core [ GPIIIa ( 423 622 ) ] domains . ^^^ Equally susceptible are the extracellular end of the transmembrane segment of both GPIIIa and the light ( L ) chain of GPIIb ( GPIIbL ) , and the N terminal end of GPIIbL . ^^^ This map of GPIIb / IIIa does not fit the current accommodation of the amino acid sequence of GPIIb and GPIIIa in the head / two tails image of the heterodimer obtained by metal rotary shadowing electron microscopy . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
To determine if autoantibodies were directed to the cytoplasmic portion of GPIIIa or to another portion of the GPIIb IIIa molecule , antibody eluted from patient platelets or plasma antibody was tested in an antigen capture assay for binding to GPIIb IIIa obtained from Chinese hamster ovary ( CHO ) cells transfected with GPIIb and either intact GPIIIa or GPIIIa lacking the carboxy terminal 35 residues . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Sequence analysis was performed after polymerase chain reaction amplification of selected patient ' s GPIIIa exons , and of the patient ' s platelet GPIIb and GPIIIa mRNAs . ^^^ This substitution points to an essential amino acid in a region of GPIIIa involved in the binding of fibrinogen and influencing the Ca ( 2+ ) dependent stability of the GPIIb IIIa complex . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Thrombocytopenia was associated with an increase in the incidence of antibodies to GPIIIa and GPIb , whereas the antigen most frequently recognized in patients without thrombocytopenia was GPIIb . ^^^ Anti GPIIb and / or GPIIIa reactivity was also seen in three out of the six anti HIV negative patients . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Analysis of the GPIIb and GPIIIa genes in patients with Glanzmann ' s thrombasthenia ] . ^^^ We analyzed the genes for GPIIb and 3 ' GPIIIa in 3 patients with GT and in 7 control subjects by Southern blot . ^^^ Furthermore , the GPIIb and GPIIIa mRNAs derived from the platelets of patients with GT could be amplified using the reverse transcriptase polymerase chain reaction ( RT PCR ) . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Using monoclonal antibodies to GPIIb , GPIIIa , and the GPIIb IIIa heterodimer , we found that the tails contained the carboxyl termini of each subunit , while the nodular head was composed of amino terminal segments of both subunits . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
In addition , this beta subunit is differently glycosylated according to whether it is associated with GPIIb or VNR alpha , one carbohydrate chain being processed to the complex type on GPIIIa , but not on VNR beta . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Initial Western blot analysis of platelet proteins from this patient did not detect any GPIIb , but did detect small amounts of GPIIIa of normal mobility . ^^^ Unlike the GPIIb gene , the GPIIIa gene appears to be intact by Southern blot analysis . ^^^ Platelet GPIIIa levels were secondarily low presumably due to the known instability of GPIIIa in the absence of GPIIb . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Polyclonal antibodies ( pab ) directed against human platelet GPIIb / IIIa and the vitronectin receptor ( VnR ) , and mouse monoclonal antibodies ( mab ) against human GPIIb , GPIIIa or the GPIIb / IIIa complex were used . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Glycoprotein IIb ( GPIIb ) is a major glycoprotein of the human platelet plasma membrane , which together with glycoprotein IIIa ( GPIIIa ) forms a Ca 2 ( + ) dependent heterodimer , GPIIb / IIIa , which serves as the major fibrinogen receptor in activated platelets . ^^^ This suggests that this region of GPIIb , somewhere between the epitope for M 5 ( alpha 550 558 ) and the epitope for M 2 ( alpha 747 755 ) , may carry the surface of interaction of GPIIb with GPIIIa in the GPIIb / IIIa heterodimer . ^^^ Finally , the sequence where the epitope for M 6 has been located ( alpha 657 667 ) was the only one found to be hydropathically complementary to the gamma 402 411 peptide of fibrinogen within the amino acid sequence of both GPIIb and GPIIIa . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
This leads us to propose that the fibrinogen binding domain of GPIIIa is not involved in the binding to GPIIb to form the Ca 2 ( + ) dependent GPIIb GPIIIa complex . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
To further evaluate the importance of the carboxyterminal region as an antigenic site , the chronic ITP plasmas were tested against Chinese hamster ovary cells transfected with GPIIb and either whole GPIIIa or GPIIIa lacking amino acids 728 to 762 . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Further studies with eight drug dependent antibodies ( DDAb ) specific for GPIIb / IIIa showed that three recognized the GPIIb / IIIa complex only , one recognized GPIIb alone , and three recognized GPIIIa alone . ^^^ The eighth serum appeared to bind to both GPIIIa alone and to an epitope determined by the GPIIb / IIIa complex . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
To determine the site of GPIIb cleavage and its role in the function of the glycoprotein IIb / IIIa heterodimer , we mutated arginine 856 , the di arginine sequence 858 859 , and arginine 871 and coexpressed the mutants with glycoprotein IIIa ( GPIIIa ) in COS 1 cells . ^^^ Because endoproteolysis most often occurs after arginines in dibasic sequences , we next expressed GPIIb mutants containing lysine at 856 or aspartic acid at 855 with GPIIIa . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Using electrophoretic techniques combined with various detection methods we ascribed rat platelet glycoproteins ( GPs ) related to human GPIb , GPIIb and GPIIIa . 2 . ^^^ Rat GPIIb and GPIIIa crossreacted with rabbit polyclonal antibodies against human GPIIb and GPIIIa . 3 . ^^^ Molecular mass of rat major GPs was determined by SDS PAGE ( unreduced , reduced , kDa ) : GPIb ( 200 , 166 / 26 ) , GPIIb ( 140 , 120 / 32 ) and GPIIIa ( 96 , 106 ) . 5 . ^^^ Isoelectric points of rat GPIIb and GPIIIa are shifted to the alkaline region as compared to human related GPs . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
The heterodimer GPIIb / IIIa , formed by the Ca ( 2+ ) dependent association of glycoproteins IIb ( GPIIb ) and IIIa ( GPIIIa ) , is the major integrin at the platelet surface , where it serves as the receptor for fibrinogen and other adhesive proteins and plays a central role in platelet aggregation and in platelet adhesion to the subendothelium . ^^^ Pure GPIIb / IIIa is obtained with a yield of about 48 % , the highest so far reported , calculated with respect to the GPIIb and GPIIIa content in the starting particulate fraction . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
This is accompanied by the appearance of membrane bound chymotryptic fragments of both glycoprotein ( GP ) IIb and GPIIIa , the two subunits of the platelet fibrinogen receptor , the GPIIb IIIa complex . ^^^ We have now evaluated the proteolysis of GPIIb IIIa by CT by Western blot analyses using a panel of polyclonal and monoclonal antibodies against GPIIb or GPIIIa . ^^^ However , incubation at 37 degrees C for longer than 10 min resulted in extensive and simultaneous degradation of both the GPIIb heavy and light chains and of GPIIIa , with the latter being converted into the 60 kDa fragment . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
The Arg Gly Asp ( RGD ) binding domain of GPIIb IIIa has been localized in a fragment of the GPIIIa subunit that includes the sequence between amino acids 109 and 171 . ^^^ Induction of AC 7 binding by D33C , a monoclonal antibody recognizing the GPIIb 426 437 sequence and stimulating fibrinogen binding , indicated that the GPIIb 426 437 and the GPIIIa 109 128 sequences were both involved in a stimulation dependent conformational modification of the receptor . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Human platelet plasma membrane glycoproteins IIb ( GPIIb ) and IIIa ( GPIIIa ) form a Ca ( 2+ ) dependent heterodimer , the integrin GPIIb / IIIa , which serves as the receptor for fibrinogen and other adhesive proteins at the surface of activated platelets . ^^^ The size exclusion chromatographic patterns of GPIIb , GPIIIa and GPIIb / IIIa have been obtained at 0 . 2 % TtX , and the molecular properties of the association products and monomer fractions have been determined by analysis of the detergent bound to the glycoproteins , laser light scattering , sedimentation velocity , and electron microscopy ( TEM ) . ^^^ GPIIIa has a greater propensity to self associate than GPIIb , this tendency being lower below 1 mg GPIIIa / ml , 0 . 1 mM Ca2+ , pH 9 . 0 . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
To determine the consequences of exon 28 deletion on the expression of the GPIIb / IIIa heterodimer , we expressed cDNA for GPIIb 28 in COS 1 cells , either individually or simultaneously with a cDNA for GPIIIa . ^^^ However , when recombinant GPIIb 28 was coexpressed with recombinant GPIIIa , GPIIb / IIIa heterodimers were assembled . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
The platelet fibrinogen receptor , which is composed of glycoproteins IIb ( GPIIb ) and IIIa ( GPIIIa ) , belongs to a large family of receptors that participate in a multitude of biologically important adhesive interactions . ^^^ Platelets from most patients with the autosomal recessive bleeding disorder , Glanzmann ' s thrombasthenia , are deficient in GPIIb and GPIIIa . ^^^ We have used cDNA probes to analyze the GPIIb and GPIIIa genes in four patients from three kindreds with Glanzmann ' s thrombasthenia . ^^^ Southern analysis of their DNA was identical to that observed in normals when probed with a full length GPIIb cDNA or a 3 ' GPIIIa cDNA . ^^^ Furthermore , we have shown that platelets can be used to characterize normal and abnormal GPIIIa and GPIIb mRNA , and RFLPs may be used to determine the carrier status in some families with Glanzmann ' s thrombasthenia . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Glycoprotein IIIa ( GPIIIa or beta 3 subunit ) is the common beta subunit of the beta 3 subfamily of integrins , which , when associated with glycoprotein IIb ( GPIIb ) , constitutes the receptor for fibrinogen and other adhesive proteins at the platelet surface ( the GPIIb IIIa complex ) and , when associated with the alpha 5 subunit , constitutes the vitronectin receptor present in several cell types . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
In this article , we describe molecular genetic studies based on use of the PCR that have allowed us to characterize platelet mRNA sequences encoding GPIIb and GPIIIa from patients in these populations . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
One gene of interest is the glycoprotein IIb ( GPIIb ) gene ; GPIIb , the alpha subunit of the platelet cytoadhesin GPIIb IIIa , is produced in megakaryocytes at an early stage of the differentiation , whereas the other subunit of this complex , GPIIIa , is expressed in other cells . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Platelet plasma membrane glycoproteins IIb ( GPIIb ) and IIIa ( GPIIIa ) form a Ca ( 2+ ) dependent heterodimer . ^^^ Using equilibrium dialysis measurements , it was established that both GPIIb and GPIIIa in solution have low affinity Ca ( 2 ) binding sites ( Kd0 . 2 0 . 3 mM ) , five in GPIIb and two in GPIIIa , and it was confirmed that only the alpha chain of GPIIb ( GPIIb alpha ) binds Ca2+ . ^^^ GPIIb / IIIa in solution has a single high affinity Ca ( 2+ ) binding site ( Kd 1 80 + / 30 nM at 21 degrees C ) , whose degree of saturation regulates the state of association of GPIIb and GPIIIa in the GPIIb / IIIa heterodimer at room temperature , and 3 4 medium affinity Ca ( 2+ ) binding sites ( Kd 2 40 + / 15 microM at 21 degrees C ) . ^^^ It is suggested that the high affinity Ca ( 2+ ) binding site could be related to the putative GPIIIa binding region in GPIIb ( residues 558 747 of the alpha chain ) . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
The phosphorylation of gpIIIa , but not gpIIb , was confirmed by the comparison of reduced and non reduced gels , and this protein was phosphorylated exclusively on tyrosine . ^^^ In contrast , both gpIIb and gpIIIa were phosphorylated when the purified complex was added to immunopurified , immobilised pp60c src . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
In addition , autoradiography of the gels showed a 125I 105 Kd GP , identified by immunoblot as GPIIIa , as well as GPIb , GPIIb , and another band at 87 Kd , probably GPIV . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
The monoclonal antibodies Tab and AP 3 are directed , respectively , against GPIIb and GPIIIa , the subunits of the platelet fibrinogen receptor . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Human platelet glycoprotein IIb ( GPIIb ) and IIIa ( GPIIIa ) form a Ca 2 ( + ) dependent heterodimer , the integrin GPIIb / IIIa , which functions as the fibrinogen receptor at the surface of activated platelets . ^^^ GPIIB and GPIIIa are synthesized as single polypeptides from single messages and their amino acid sequences were derived from their cDNAs . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
As shown by immunoblotting experiments , treatment of such platelets with HNE ( 14 micrograms / ml ) did not provoke a detectable cleavage of GPIIb but resulted in a partial digestion of GPIIIa and appearance of 110 kDa fragment . ^^^ Treatment of partially activated platelets ( spontaneous activation during washing procedure ) with HNE caused a progressive loss of GPIIb and degradation of GPIIIa to 110 kDa and 60 kDa fragments . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
To understand this apparently nonfunctional hyalomere GPIIb IIIa further , correlative immunocytochemistry was performed using polyclonal and monoclonal antibodies for GPIIb and GPIIIa simultaneously . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Glycoproteins GPIb , GPIIb , GPIIIa , and other 25 260 kilodalton ( kDa ) proteins were identified as target antigens , suggesting a transient autoimmune mechanism causing the thrombocytopenia . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
This location was found with three monospecific polyclonal antibodies : one anti GPIIb IIIa antibody , the second specific for GPIIb , and the third specific for GPIIIa . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Knowledge of the sequence and organization of the GPIIb and GPIIIa genes will help in understanding evolutionary relationships and functional homologies of this family of adhesion protein receptors and will facilitate analysis of molecular defects responsible for thrombasthenia . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Glycoproteins IIb ( GPIIb ) and IIIa ( GPIIIa ) form the Ca 2 ( + ) dependent GPIIb / IIIa complex , which acts as the fibrinogen receptor on activated platelets . ^^^ GPIIb and GPIIIa are synthesized as single peptide chains . ^^^ The GPIIb / IIIa complex has two membrane attachment sites located at the C termini of GPIIb beta and GPIIIa . ^^^ The short cytoplasmic tails of GPIIb beta and / or GPIIIa become most likely associated to the cytoskeleton of activated platelets . ^^^ In the present work the C terminal amino acid residues of platelet GPIIb beta and GPIIIa have been analyzed by protein chemical methods and compared with those predicted from cDNA analysis . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
GPIIb and GPIIIa amino acid sequences deduced from human megakaryocyte cDNAs . ^^^ The sequence for GPIIb and GPIIIa have recently been derived from cDNAs obtained from HEL cells . ^^^ This study describes full length cDNAs for GPIIb and GPIIIa isolated from megakaryocyte cDNA libraries . ^^^ The mRNA species for GPIIb ( 3 . 4 kb ) and GPIIIa ( 6 . 1 kb ) were of the same size in HEL cells and human megakaryocytes . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
The earliest marker of the megakaryocyte lineage in human bone marrow is a heterodimeric complex of glycoproteins IIb ( GPIIb ) and IIIa ( GPIIIa ) . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
These antibodies recognized GPIIb in two cases , GPIIIa in one case , and an unidentified platelet protein of 150 kDa in the three other cases . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
With purified GPIIb : IIIa as an antigen , we have produced monoclonal antibody CS 1 , which immunoblotting demonstrates to be specific for native GPIIIa ; disulfide bond reduction of GPIIIa resulted in loss of immunoreactivity . ^^^ Since CS 1 inhibits fibrin ( ogen ) interactions with GPIIb : IIIa , we propose that the conformationally dependent epitope on GPIIIa recognized by CS 1 constitutes a region of the receptor which is involved in fibrin ( ogen ) binding . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
None of them reacted with dissociated GPIIb or GPIIIa . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Polyclonal antibodies from human origin directed against PLA 1 , Leka antigens , and the GPIIb IIIa complex ( IgGL ) , or murine monoclonal antibodies specific for GPIb ( AN 51 , 6D1 ) , GPIIIa ( AP 3 ) , or GPIIb IIIa ( AP 2 ) were studied using platelet suspension immunofluorescence tests . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
The GPIIb and GPIIIa subunits were both present and were of similar molecular weight in these cell types . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
By ELISA wherein monoclonal antibodies specific for GPIIb ( Tab ) and specific for GPIIIa ( AP 3 ) were used to capture and hold antigens from a platelet lysate prepared under conditions that generate free GPIIb and GPIIIa , anti Pena reacted with GPIIIa held by AP 3 but not with GPIIb held by Tab . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
In addition , we have determined the chromosomal location of the gene for the beta subunit of the GPIIb / IIIa heterodimer , GPIIIa . ^^^ In contrast to the chromosomal dispersion of the alpha subunits , the genes for GPIIb and GPIIIa are physically close , with the gene for GPIIIa also located on chromosome 17q21 23 . ^^^ These studies indicate that the genes for the alpha subunits of the integrin family have been dispersed during evolution while GPIIb and GPIIIa are in close physical proximity . ^^^ This physical proximity of GPIIb and GPIIIa may be involved in the concurrent expression of these proteins by megakaryocytes , and may result in linkage disequilibrium between these two genes , which would limit the use of restriction length polymorphisms in linkage studies of GPIIb / IIIa abnormalities in small kindreds . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
In contrast , in the presence of 2 mM Ca2+ and 1 mM Mg2+ , 5G11 precipitated a complex of five radiolabeled proteins , among which GPIIb , GPIIIa and GPIV were the most prominent . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Amino acid analysis showed values comparable with those deduced from the cloning data for GPIb , GPIIb , and GPIIIa . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Biosynthesis and assembly of platelet GPIIb IIIa in human megakaryocytes : evidence that assembly between pro GPIIb and GPIIIa is a prerequisite for expression of the complex on the cell surface . ^^^ The platelet membrane glycoproteins GPIIb and GPIIIa form a calcium dependent heterodimer that functions as a receptor for adhesive proteins on stimulated platelets . ^^^ Pulse chase experiments analysis performed on human megakaryocytes obtained from liquid cultures of chronic myelogenous leukemic patients with antibodies specific for GPIIIa or GPIIb demonstrated the existence of a pro GPIIb GPIIIa complex and of a large pool ( 60 % ) of unassociated GPIIIa ; nearly all the GPIIb and the pro GPIIb molecules were found associated with GPIIIa . ^^^ The expression of GPIIIa in PMA treated cells resulted in the production of the mature GPIIb form and the expression of the GPIIb IIIa complex on the cell surface . ^^^ These results indicate that assembly between the early forms of pro GPIIb and GPIIIa is an obligatory step for the maturation of the heterodimer and its expression on the cell surface . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Both GPIIb and GPIIIa were synthesized as single chain precursors to which high mannose N linked oligosaccharides were added in the endoplasmic reticulum ( ER ) . ^^^ Assembly of the GPIIb and GPIIIa precursors required 4 6 h for completion . ^^^ All GPIIIa molecules were eventually assembled ; the excess GPIIb precursors were degraded without reaching the cell surface . ^^^ These studies suggest that 1 ) assembly of the precursors of GPIIb and GPIIIa in the ER is a slow process requiring conformational maturation of one or both subunits , and 2 ) only heterodimers assembled in the ER are transported to the Golgi apparatus for additional processing and , ultimately , expression on the cell surface . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
As measured by monoclonal antibody binding in the presence of divalent cations , the platelets contained about 15 % of the normal amount of GPIIb and GPIIIa and only 6 % of the normal amount of intact GPIIb / IIIa complex . ^^^ This form of thrombasthenia is characterized by a defective platelet function , a marked reduction of GPIIb and GPIIIa , decreased stability of the residual GPIIb / IIIa complex and structural heterogeneity of GPIIb . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
The platelet receptor for fibrinogen is a macromolecular complex , GPIIb IIIa , made of two transmembrane glycoproteins , GPIIb ( Mr = 142 , 000 ) and GPIIIa ( Mr = 99 , 000 ) , assembled into a heterodimer whose conformation depends upon the binding of calcium ( Ca2+ ) to GPIIb . ^^^ Binding of fibrinogen to GPIIb IIIa occurs through interactions between peptide sequences within the receptor and particular adhesive sites within the ligand : thus , GPIIIa can bind to a tetrapeptide sequence of the A alpha chain of fibrinogen , whereas a dodecapeptide of the gamma chain can be preferentially bound to GPIIb . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
To determine whether GPIIb ( H ) or GPIIb ( L ) remained complexed with GPIIIa , reduced platelet lysates were immunoprecipitated with AP 3 , a monoclonal anti GPIIIa antibody , resulting in the coimmunoprecipitation of GPIIb ( H ) but not GPIIb ( L ) . ^^^ Conversely , the monoclonal anti GPIIb ( H ) antibody PMI 1 immunoprecipitated GPIIIa with GPIIb ( H ) . ^^^ Thus GPIIb ( H ) maintains its association with GPIIIa . ^^^ These findings indicate that GPIIb ( H ) contains sufficient information for maintenance of a complex with GPIIIa and support of the binding of the heterodimer to RGD peptides . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
However , the addition of agonistic concentrations of either monoclonal antibody before cross linking results in the coprecipitation of proteins corresponding in mobility and peptide composition to GPIIb , and GPIIIa . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
The two major lipoprotein binding membrane glycoproteins were purified to apparent homogeneity and identified by their mobilities in sodium dodecyl sulfate polyacrylamide gel electrophoresis , both in the nonreduced and reduced state , by specific antibodies against glycoproteins IIb ( GPIIb ) and IIIa ( GPIIIa ) , respectively , including the alloantibody anti PlA 1 and monoclonal antibodies . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
GPIb ( four ) , GPIIb ( one ) and GPIIIa ( five ) . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Coexpression of both subunits resulted in a proteolytically processed form of GPIIb that associated with GPIIIa as a heterodimeric complex as the cell surface . ^^^ When expressed alone , the GPIIb subunit remained intracellular , while the GPIIIa subunit was found to complex with endogenous proteins and was mobilized to the cell surface . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Binding of a murine monoclonal antibody directed at GPIIb and / or GPIIIa to intact thrombasthenic platelets averaged less than 2 % of the control value . ^^^ These studies indicate that the thrombasthenics in Israel have the severe form of the disease ( type 1 ) and that the platelets of heterozygotes have significantly reduced amounts of both total and surface exposed GPIIb and / or GPIIIa . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Metabolic labeling of endothelium with [ 35S ] methionine demonstrated that both GPIIb and GPIIIa were actively synthesized in culture . ^^^ Using the technique of crossed immunoelectrophoresis , evidence was obtained that the endothelial cell forms of GPIIb and GPIIIa may exist complexed to one another after solubilization in Triton 10 100 . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Using platelets from patients with Glanzmann ' s thrombasthenia or Bernard Soulier syndrome and purified GPIIIa , these proteins were shown to be GPIb , GPIIb , GPIIIa , GPIX , and an unidentified 57 Kd protein missing in Bernard Soulier syndrome . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Considerable evidence indicates that the glycoprotein ( GP ) IIb / IIIa complex on human platelets functions as a receptor for fibrinogen , but little is known about the mechanism of receptor `` exposure . ' ' To investigate this mechanism , our previously described murine monoclonal antibody ( 10E5 ) and a new monoclonal antibody ( 7E3 ) , both of which block the binding of fibrinogen to platelets and bind to GPIIb and / or GPIIIa , were radiolabeled and their rates of binding to native and ADP activated platelets were studied . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
The quantity of iodine 125 labeled glycoprotein IIb ( GPIIb ) and glycoprotein IIIa ( GPIIIa ) on the platelets of these patients was considerably less than normal but was detectable by immunoprecipitation , sodium dodecyl sulfate polyacrylamide gel electrophoresis , and autoradiography . ^^^ The amount of residual GPIIb and GPIIIa as measured by binding studies with radiolabeled monoclonal antibodies was between 3 % and 12 % of the normal value . ^^^ Monoclonal antibodies specific for the GPIIb GPIIIa complex blocked both the fibrinogen binding and the aggregation of enzyme treated platelets . ^^^ However , the level of GPIIb and GPIIIa on these platelets as measured by a Western blot technique and by monoclonal antibody binding amounted to less than 0 . 35 % to 0 . 5 % of normal values . ^^^ In conclusion , fibrinogen binding sites exposed on thrombasthenic platelets by chymotrypsin are derived from GPIIb GPIIIa molecules . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
We conclude that surface proteolysis by low concentrations of elastase can expose fibrinogen binding sites associated with GPIIb and GPIIIa on the platelet surface , resulting in spontaneous aggregation upon the addition of fibrinogen . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Congenital absence of platelet glycoproteins IIb and IIIa ( GPIIb and GPIIIa ) results in a severe bleeding disorder characterized by defective platelet aggregation and failure of fibrinogen to bind to platelets . ^^^ GPIIb and GPIIIa are present as a heterodimeric , noncovalent complex in the platelet plasma membrane and function as the fibrinogen receptor . ^^^ To characterize synthesis of these two proteins , RNA isolated from a human leukemia cell line that contains GPIIb and GPIIIa was translated in a wheat germ cell free system . ^^^ Polyclonal antibodies specific for each protein immunoprecipitated distinct [ 35S ] methionine labeled precursors , indicating that GPIIb and GPIIIa are translated from separate mRNAs . ^^^ These results suggest that precursors of GPIIb and GPIIIa may be encoded by separate genes and that each precursor is processed before delivery to the plasma membrane . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Monoclonal antibodies , specific for GPIIIa and GPIIb / IIIa , respectively , bound normally to the P1A1 positive platelets from the patient . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Although neither AP 3 , which is directed against GPIIIa , nor Tab , which is specific for GPIIb , were individually able to inhibit adenosine diphosphate ( ADP ) induced fibrinogen binding , platelet aggregation , or secretion , the combination of AP 3 and Tab completely abolished platelet aggregation and the release reaction . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
The polypeptide backbones of the GPIIb and the GPIIIa have molecular masses of 120 and 90 kD , respectively . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
A murine monoclonal antibody specific for glycoprotein ( GP ) IIIa was prepared by immunization with a GPIIb and GPIIIa enriched Triton 10 114 extract of platelet membranes . ^^^ The epitope on GPIIIa recognized by AP 3 is expressed on dissociated GPIIIa as well as on Ca+2 dependent complexes of GPIIb and GPIIIa , as shown by crossed immunoelectrophoresis in the presence or absence of EDTA . ^^^ Thus , data derived using AP 3 indicate that significant amounts of free GPIIIa are not present , thereby supporting the hypothesis that GPIIb and GPIIIa exist complexed in a 1 : 1 stoichiometry in the plasma membrane of intact , nonactivated platelets . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Platelet aggregation requires the binding of adhesive proteins such as fibrinogen to the heterodimer of membrane glycoproteins IIb ( GPIIb ) and IIIa ( GPIIIa ) . ^^^ Human erythroleukemia ( HEL ) cells synthesize both GPIIb and GPIIIa . ^^^ We localized the gene for GPIIIa to chromosome 17 , the same chromosome to which we had previously mapped the gene for GPIIb . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Most patients affected by Glanzmann thrombasthenia have low levels of GPIIb and GPIIIa ; however , the separate mechanisms responsible for the deficiency in each remain to be determined . cDNA clones coding for the GPIIb and GPIIIa have been recently isolated , and their corresponding genomic sequences have been colocalized to the long arm of chromosome 17 . ^^^ The similarity of banding patterns among probands , family members , and controls indicated that there were no major insertions or deletions in either the GPIIb or GPIIIa genes . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
The fibrinogen receptor on human platelets is a prototypic member of the integrin family and is composed of subunit glycoproteins IIb ( gpIIb ) and IIIa ( gpIIIa ) in a 1 : 1 stoichiometric ratio . ^^^ We have isolated cDNA clones for gpIIb and gpIIIa and localized both genes to chromosome 17 . ^^^ In the current study , several approaches were used to localize and map the genes for gpIIb and gpIIIa . ^^^ Finally , nonoverlapping portions of the cDNAs for both gpIIb and gpIIIa were used to probe Sfi 1 digests of genomic DNA separated by field inversion gels . ^^^ This confirmed that the genes are physically linked within the same 260 kilobase Sfi 1 fragment and suggests that the gene for gpIIb is located on the 3 ' side of the gene for gpIIIa . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
GPIIb IIIa is a heterodimer complex composed of GPIIb ( the alpha subunit ) , which consists of two disulfide linked heavy and light chains , and GPIIIa ( the beta subunit ) , which is a single polypeptide chain . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
We have investigated the target antigens recognized by anti platelet antibodies in patients with chronic idiopathic thrombocytopenic purpura ( ITP ) using an immunoblot procedure which could electrically separate the glycoprotein ( GP ) IIb / IIIa complex into GPIIb and GPIIIa . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Cross linking of the labeled RGD peptides to GPIIb and GPIIIa on stimulated and nonstimulated platelets exhibited structural specificity and was inhibited by excess nonlabeled RGD peptides . ^^^ These results indicate that the RGD binding sites on platelets reside in close proximity to both subunits of GPIIb IIIa and that platelet stimulation alters the topography of these sites such that the peptides become more efficiently cross linked to GPIIIa . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Crossed immunoelectrophoresis of the patient ' s platelets demonstrated the formation of a GPIIb / IIIa complex that was mainly composed of normal mol wt GPIIb and GPIIIa . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
With the use of a polyclonal antiplatelet membrane antibody , the glycoproteins IIb ( GPIIb ) , IIIa ( GPIIIa ) , and a 60 , 000 Da ( 60 kDa ) protein ( 66 kDa in a reduced system ) derived from GPIIIa were immunoprecipitated from the surface of detergent extracts of human 125I radiolabeled platelets pretreated with increasing concentrations of human granulocyte elastase . ^^^ GPIIIa appears to be the preferred substrate of elastase , since GPIIb was not degraded by human granulocyte elastase . ^^^ We conclude that 1 ) the proteolytic action of human granulocyte elastase on platelet GPIIIa results in the formation of two major hydrolytic products , and 2 ) human granulocyte elastase exposes active fibrinogen binding sites associated with the GPIIb / GPIIIa complex , resulting in direct platelet aggregation by fibrinogen . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Both GPIIb and GPIIIa are synthesized by a human erythroleukemia ( HEL ) cell line . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Human and bovine cultured cell lines and circulating leukocytes were examined for the presence of surface proteins similar to platelet glycoproteins IIb ( GPIIb ) and IIIa ( GPIIIa ) . ^^^ Human endothelial cells , smooth muscle cells , and MG 63 fibroblast like cells were found to have surface proteins that cross reacted with platelet GPIIb and GPIIIa antibodies , existed as complexes , and had molecular weights similar to those of the corresponding platelet glycoproteins . ^^^ Bovine endothelial cells and smooth muscle cells also expressed GPIIb and GPIIIa like surface proteins . ^^^ The GPIIIa like protein in human endothelial and smooth muscle cells had the same isoelectric point as platelet GPIIIa , whereas their GPIIb alpha like protein was slightly more acidic than platelet GPIIb alpha ( pI = 5 . 2 5 . 3 versus 5 . 5 ) . ^^^ Platelet and endothelial cell GPIIb alpha ( but not GPIIIa ) showed an increased electrophoretic mobility in Ca2+ containing versus EDTA containing gels , implying a Ca2+ GPIIb alpha interaction . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
The platelet membrane glycoproteins IIb ( GpIIb ) and GpIIIa form calcium dependent heterodimers containing binding sites for fibrinogen , von Willebrand factor , and fibronectin . ^^^ Although GpIIb and GpIIIa are distinct proteins , both GpIIb and GpIIIa are deficient in platelets from individuals with the recessive disorder Glanzmann ' s thrombasthenia . ^^^ To gain a better understanding of the genetic basis for GpIIb and GpIIIa synthesis , we studied their synthesis by two human leukemia cell lines , HEL and K 562 . ^^^ HEL cells contained complexes of GpIIb and GpIIIa , and K 562 cells expressed GpIIIa , but not GpIIb , when stimulated with phorbol 12 myristate 13 acetate ( PMA ) . ^^^ RNA from HEL cells directed the in vitro synthesis of a 110 , 000 Mr precursor for GpIIb and a 92 , 000 Mr precursor for GpIIIa , which indicates that the synthesis of GpIIb and GpIIIa by HEL cells is directed by separate mRNAs . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Sedimentation equilibrium and low angle laser light scattering were used to determine the molar mass of the glycoprotein moieties in the complexes of sodium dodecyl sulphate with the human platelet membrane glycoproteins IIb ( GPIIb ) , IIIa ( GPIIIa ) , and the alpha ( GPIIb alpha ) and beta ( GPIIb beta ) subunits of GPIIb . ^^^ The values obtained by both procedures , except those for GPIIb , agree within experimental error with those calculated from their chemical composition : GPIIb alpha ( 114 , 000 g mol 1 ) , GPIIb beta ( 22 , 200 g mol 1 ) , and GPIIIa ( 91 , 500 g mol 1 ) . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
The four antibodies also promoted drug dependent precipitation of GPIIb and GPIIIa from lysates of radioiodinated Bernard Soulier platelets . ^^^ Absorption of a quinine induced antibody with Bernard Soulier platelets in the presence of drug eliminated its ability to precipitate GPIIb and GPIIIa . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
The number of 125I trigramin binding sites on thrombasthenic platelets amounted to 2 . 7 5 . 4 % of control values obtained for normal platelets and correlated with the reduced number of GPIIb GPIIIa molecules on the platelet surface . ^^^ EDTA , monoclonal antibodies directed against the GPIIb GPIIIa complex , and synthetic peptides ( Arg Gly Asp Ser and Tyr Gly Gln Gln His His Leu Gly Gly Ala Lys Gln Ala Gly Asp Val ) blocked both 125I fibrinogen binding and 125I trigramin binding to platelets . ^^^ Monoclonal antibodies directed either against GPIIb or GPIIIa molecules did not block the interaction of either ligand with platelets . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Effect of charge on association and dissociation of the glycoprotein GPIIb GPIIIa membrane complex . ^^^ The mechanism of association of the human platelet membrane GPIIb GPIIIa Ca2+ complex was studied by treating solubilized membranes with various enzymes and cationic peptides and by studying the binding of 45Ca2+ and 125I fibrinogen before and after dissociation with EGTA and association with Ca2+ . ^^^ Thus , critical neuraminidase sensitive components of the complex ( sialic acid residues ) are not exposed on the surface of the platelet membrane of resting platelets , but do become accessible following platelet stimulation with ADP or membrane solubilization with Triton 10 100 . 45Ca2+ bound to the associated complex , to GPIIb of the dissociated complex ( not to GPIIIa ) , to the Ca2+ reassociated complex , and to the neuraminidase associated complex which had been dissociated with EGTA . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Human platelet membrane glycoproteins IIb ( GPIIb ) and IIIa ( GPIIIa ) , which have been proposed to be subunits of a receptor for fibrinogen , were purified from Triton 10 100 solubilized platelet membranes by affinity chromatography on a concanavalin A ( Con A ) Sepharose column followed by preparative sodium dodecyl sulfate polyacrylamide gel electrophoresis . ^^^ Compositional analyses of the purified glycoproteins showed that GPIIb and GPIIIa contain 15 % and 18 % carbohydrate by weight , respectively , which consists of galactose , mannose , glucosamine , fucose , and sialic acid . ^^^ The neutral oligosaccharides , which comprised about 14 % of the total oligosaccharides released from GPIIb and about 52 % of that from GPIIIa , were found to be of the high mannose type , in that they contained 5 or 6 mannose residues . ^^^ In conclusion , we found that GPIIb contained mainly complex type sugar chains , whereas high mannose type sugar chains were the predominant carbohydrate units in GPIIIa , and that the detected differences in the carbohydrate moieties of GPIIb and GPIIIa were quantitative but not qualitative . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
We describe a new procedure for isolation of glycoproteins IIb ( GPIIb ) and IIIa ( GPIIIa ) from human platelet plasma membrane with high yields ( 2 . 7 mg of GPIIb and 3 . 3 mg of GPIIIa per 100 mg of starting platelet membrane proteins ) , equivalent to a recovery of 35 % and 55 % respectively of the total GPIIb and GPIIIa of the membrane . ^^^ The weight percentage of sugar is 15 . 7 % for GPIIb and 12 . 5 % for GPIIIa . ^^^ Neuraminic acid is present in both glycoproteins , representing 30 % and 15 % respectively of the total sugar weight of GPIIb and GPIIIa . ^^^ The molar percentages of half cystine and methionine are 4 fold and 2 fold higher respectively in GPIIIa than in GPIIb . ^^^ The Mr values obtained , 136 , 500 for GPIIb and 91 , 500 for GPIIIa , are in very good agreement with those obtained by physical methods . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
The use of this technique should aid in defining the structural and functional properties of GPIIb and GPIIIa . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Microtiter wells , coated with a monoclonal antibody ( 2G12 ) specific for GPIIb / GPIIIa were reacted with GPIIb / GPIIIa contained in a platelet extract . ^^^ Control wells containing the same antibody were reacted with a cell extract containing no GPIIb / GPIIIa . ^^^ Assay values were expressed as binding ratios ( cpm GPIIb / GPIIIa wells / cpm control wells ) . ^^^ Autoradiographs from SDS PAGE electrophoresis of the Staph A bound proteins shows two radioactive bands consistent in size with GPIIb and GPIIIa . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
In addition , we find that thrombasthenic platelets , which are deficient in glycoproteins IIb and IIIa ( GPIIb and GPIIIa ) contain normal amounts of alpha actinin , confirming the recent finding that alpha actinin and GPIIIa are different proteins in human platelets . ^^^ Thus , the thrombasthenic defect in GPIIb and GPIIIa does not diminish the content of either alpha actinin or vinculin . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Glycoprotein IIb ( GPIIb ) and glycoprotein IIIa ( GPIIIa ) form a macromolecular complex on the activated platelet surface which contains the fibrinogen binding site necessary for normal platelet aggregation . ^^^ To identify the specific region of the fibrinogen molecule responsible for its interaction with the GPIIb GPIIIa complex , purified fragment D 1 ( Mr = 100 , 000 ) and fragment E ( Mr = 50 , 000 ) were prepared from plasmin digests of purified human fibrinogen . ^^^ Using an enzyme linked immunosorbent assay we have demonstrated that isolated fragment D 1 in a solid phase system forms a complex with a mixture of GPIIb and GPIIIa . ^^^ The binding of the GPIIb GPIIIa mixture to fragment D 1 coated plates reached saturation at 8 nM and to fibrinogen coated plates at 24 nM . ^^^ Fragment E coated directly on plastic plates or immobilized on antibody coated plastic plates did not form a complex with GPIIb GPIIIa . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
With 3B2 the mean of the OD ratios of five patients with autoimmune thrombocytopenic purpura in remission ( greater than 150 , 000 platelets per mm 3 ) compared to controls was 0 . 80 + / 0 . 14 . 3B2 did not react with platelets from a patient with Glanzmann ' s thrombasthenia , in which membranes lack glycoproteins IIb and IIIa ( GPIIb and GPIIIa ) . ^^^ Platelet membranes were run on crossed immunoelectrophoresis against a rabbit polyclonal anti human platelet membrane antibody with 125I labeled purified 3B2 in an intermediate spacer gel . 3B2 reacted with the GPIIb GPIIIa Ca2+ complex in the presence of excess Ca2+ and with GPIIb alone in the presence of excess EGTA . ^^^ Thus , the reactivity of monoclonal antibody 3B2 with GPIIb or the GPIIb GPIIIa Ca2+ complex appears to be inhibited by the presence of autoantibody on platelets . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Using these monospecific antisera in an enzyme linked immunosorbent assay system , it is no demonstrated that glycoprotein IIb ( GPIIb ) and glycoprotein IIIa ( GPIIIa ) form a complex with purified human fibrinogen . ^^^ The formation of this GPIIb GPIIIa fibrinogen complex is calcium dependent , fibrinogen specific , saturable , and inhibited by specific amino sugars and amino acids . ^^^ These observations suggest that the GPIIb GPIIIa macromolecular complex on the platelet surface acts under the proper physiologic circumstances as the fibrinogen binding site required for normal platelet aggregation . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
The major antigen consists of a complex of glycoproteins , GPIIb and GPIIIa , held together by Ca2+ and missing in Glanzmann ' s thrombasthenia . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Cotransfection of Asp 418 GPIIb and GPIIIa plasmid constructs into COS 7 cells resulted in the accumulation of a pre GPIIb IIIa complex that failed to reach the cell surface , in effect recreating the thrombasthenic phenotype . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Of the two candidate GPIIIa sequences , 118 131 and 208 221 , the former bound Tb3+ and divalent cations with an affinity similar to that of the GPIIb peptides , whereas the latter peptide was not functional . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
The proband ' s platelets bound more antibodies against the vitronectin receptor alpha 5 beta 3 than normal platelets , suggesting a normal GPIIIa ( beta 3 ) gene and a defect in the GPIIb gene . ^^^ Cotransfection of COS 7 cells with expression vectors containing wild type GPIIIa cDNA and the mutated GPIIb cDNA failed to produce detectable amounts of GPIIb / IIIa on the surface of the cells . ^^^ The observed mutation is in a region that is conserved from rodents to humans and has been suggested to be involved in the interaction between GPIIb and GPIIIa when these GPs are complexed in solution . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Furthermore , synthesis of mRNA for GPIIb and GPIIIa by CD34+ cells was shown using PCR analysis . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
We describe a solid phase assay that specifically detects antibodies to platelet membrane associated alloantigens by measuring the ability of patient antisera to inhibit the binding of glycoprotein GPIIb or GPIIIa monoclonal antibodies to intact platelets . ^^^ In conclusion , the platelet monoclonal antibody inhibition assay : ( 1 ) allows determination of platelet specific alloantibodies in the presence of contaminating HLA antibodies and / or in sera containing multiple platelet alloantibodies ; ( 2 ) allows accurate platelet phenotyping for the GPIIIa associated PLA and GPIIb associated Bak antigen systems ; and ( 3 ) may be applicable to the detection of other known or even novel platelet glycoprotein associated antigens . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Antibodies were preincubated with purified intact GPIIb / IIIa , EDTA dissociated GPIIb / IIIa , GPIIIa , or GPIIb for 2 hours and then residual antibody was measured in an antigen capture assay . ^^^ Little inhibition was noted using either GPIIb or GPIIIa . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Preincubation of normal PRP with monoclonal anti GPIIb / IIIa or anti GPIb antibodies completely or partially inhibited the Apt 4 induced aggregation , whereas anti GPIIIa antibodies have no effects on this activation . ^^^ Immunoprecipitation of 125I labeled platelet membrane lysate by Apt 4 IgG showed two protein bands with a molecular weight of 145 , 000 and 95 , 000 daltons respectively under non reducing condition , which are corresponding to GPIIb and GPIIIa . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Flow cytometric as well as immunoblot analysis of patient platelets showed significantly reduced levels of GPIIb and GPIIIa compared with a normal control . ^^^ The recombinant His 327 form of GPIIb was found to be fully capable of associating with GPIIIa , therefore the role of the calcium binding domains in intersubunit association was further examined by constructing amino terminal segments of GPIIb that ended before the first , second , and third calcium binding domains . ^^^ All three fragments were found to associate with GPIIIa , demonstrating that the calcium binding domains of GPIIb are not necessary for initial complex formation . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
This is non radioactive PCR SSCP ( single strand conformation polymorphism ) , which we illustrate for two different HPA systems , one on GPIIIa ( HPA 1 ) and the other on GPIIb ( HPA 3 ) . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Further , while GPIIIa is expressed in other tissues as part of the vitronectin receptor , GPIIb is only expressed on maturing megakaryocytes and the platelets derived from them . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Chem . 266 , 13627 13633 ) . 8 azido ATP has now been shown to have similar binding parameters ( Kd 8 nM , 20 , 000 sites / platelet ) but , in this case , photoincorporation occurred equally in GPIIb and GPIIIa . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Immunoprecipitation of radiolabeled platelets using polyclonal anti FXIII A chain antibody identified two proteins corresponding to GPIIb and GPIIIa . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
In addition , they included the major surface glycoproteins GPIIb and GPIIIa without noticeable contamination of soluble cytosol proteins . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
In this study employing a sensitive immunoblot assay , we have characterized GPIIb and GPIIIa in thrombasthenic platelets from seven type 2 and four type 1 patients from 10 unrelated families . ^^^ The amounts of GPIIb and GPIIIa were both markedly reduced in all these patients , and abnormal molecular weight GPIIb or GPIIIa was not detected . ^^^ In all of four type 1 patients the amount of GPIIb was much lower than that of GPIIIa . ^^^ Immunodepletion of patients ' platelets with AP 2 ( a monoclonal antibody specific for the GPIIb IIIa complex ) , AP 3 ( specific for GPIIIa ) or AMF 7 ( specific for alpha 5 ) further confirmed that GPIIIa existed in excess , and demonstrated that excess GPIIIa were mostly in free form and not associated with GPIIb or alpha 5 . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
We identified the 80 100 , 130 150 and 150 170 KD surface proteins that comigrate with GPIIIa , GPIIb and GPIb and a 50 70 KD cytoplasm band by immunoblot . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
The defective expression on the surface of DNA cotransfected COS 1 cells with GPIIb 28 and GPIIIa cDNAs was described in an earlier report . ^^^ Western blotting showed that the patients ' platelets had defective GPIIb and have trace amounts of GPIIIa . ^^^ Sequence analysis was performed after polymerase chain amplification of the patients ' GPIIb and GPIIIa mRNAs . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Comparison of their fragmentation patterns shows that , whereas in the non RGD binding form of GPIIb / IIIa the N terminal half of the heavy chain of GPIIb ( GPIIbH ) and the central region of GPIIIa are cleaved by endoproteinase Arg C , these domains associate tightly with one another in the RGD binding GPIIb / IIIa and are thus protected from proteolysis . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Through the use of immunofluorescence light microscopy and immunogold electron microscopy in conjunction with monospecific , polyclonal antibodies , GPIIb and GPIIIa cross reacting antigens were identified on membranes of monocyte / macrophages cultured from White Carneau pigeons . ^^^ Immunoblotting of membranes isolated from pigeon monocyte / macrophages identified a protein of 132 , 000 M ( r ) that was recognized by anti GPIIb and a protein of 114 , 000 M ( r ) that was recognized by anti GPIIIa . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
This study shows the retention of GPIIb and GPIIIa on immobilized fibrinogen after Triton 10 100 ( Sigma Chemical Co , St Louis , MO ) lysis of adherent platelets . ^^^ Platelet exposure to adenosine diphosphate or thrombin , but not A 23187 or chymotrypsin , markedly enhanced GPIIb and GPIIIa recovery relative to that observed with unstimulated platelets , or prostaglandin E 1 treated platelets . ^^^ Moreover , lysis of adherent platelets in the presence of 10 mmol / L EDTA , under conditions promoting GPIIb IIIa complex dissociation ( pH 8 . 1 , 60 minutes , 37 degrees C ) , had no effect on GPIIb or GPIIIa subunit recovery . ^^^ Platelet activation with Zn+2 also enhanced GPIIb and GPIIIa recovery on fibrinogen coated surfaces over that observed with unstimulated platelets , but GPIIb and IIIa retention was EDTA sensitive . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Circulating immune complexes ( CIC ) were the main factor in the inhibition of GPIIb / IIIa binding to fibrinogen in HIV related ITP , whereas in non HIV ITP , inhibition was only partially due to CIC ; anti platelet antibodies specific to GPIIIa were also shown to play a role . ^^^ We also observed a decrease and / or disappearance of the antibodies specific to GPIIb and / or GPIIIa after IVIG administration . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
We have examined how this actin interacts with the membrane skeletal fraction and find that the actin is not associated with the membrane directly or indirectly through any of the major transmembrane glycoproteins ( GpIb , GpIIb , and GpIIIa ) , or any known specific linker proteins such as actin binding protein ( ABP ) , alpha actinin , or spectrin . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
The platelet fibrinogen receptor is a heterodimer composed of glycoproteins IIb ( GPIIb ) and IIIa ( GPIIIa ) . ^^^ Platelets from patients with GT have a quantitative or qualitative abnormality in GPIIb and GPIIIa and can neither bind fibrinogen nor aggregate . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
The platelet glycoproteins GPIIb and GPIIIa are integral membrane proteins and form calcium dependent heterodimers in the endoplasmic reticulum ( ER ) . ^^^ In the absence of heterodimer formation , GPIIb and GPIIIa are retained in the ER and degraded . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Using double labeling studies with polyclonal anti GPIIb antibodies coupled to 10 nm gold particles and polyclonal anti GPIIIa antibodies coupled to 20 nm gold particles , we observed that while both 10 and 20 nm particles were present in the dilated portions of the canaliculi almost only the small particles , coupled to the anti GPIIb antibodies , labeled the collapsed portions of the SCCS . ^^^ On Lowicryl thin sections , polyclonal antibodies against GPIIb labeled the central striated zone while both GPIIb and GPIIIa were found in the dilated portions of the SCCS . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Western blotting gave an apparent molecular weight for GPIb of 160 , 000 , while GPIIb was 135 , 000 and GPIIIa was 88 , 000 . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Peptides derived from a sequence within the loop structure of human platelet glycoprotein ( GP ) IIIa ( integrin beta 3 ) were previously shown to inhibit fibrinogen binding to purified GPIIb IIIa . ^^^ These GPIIIa peptides also inhibited the binding of two monoclonal antibodies , pl 55 and PAC 1 , which are directed against the activated conformer of GPIIb IIIa . ^^^ Only GPIIb IIIa was retained on the column , and it could be specifically eluted by GPIIIa peptide or RGDV but not by an irrelevant peptide . ^^^ Additionally , we observed that the binding of GPIIIa peptides to purified GPIIb IIIa induced exposure of a neoepitope on GPIIb that was recognized by the monoclonal antibody pl 80 . ^^^ These data suggest that sequences within the loop structure of GPIIIa can interact with the ligand binding domain of GPIIb IIIa . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
The Western blot analysis showed that GPIIIa at the platelet surface was apparently deficient , it may be ascribed to the rapid turn over of GPIIIa uncomplexed with the truncated GPIIb . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Flow cytometry and immunoblotting revealed normal amounts of GPIIb and diminished GPIIIa ( 50 % of control ) . ^^^ The parents had moderately reduced GPIIb / IIIa function in this assay , but they did not demonstrate a reduced GPIIIa as was noted in the patient . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Moreover , preincubation of r 293 clone B cells with a monoclonal antibody ( mAb ) specific for GpIIIa ( 4B12 ) resulted in a dose dependent decrease of binding to fibrinogen while a mAb specific for GPIIb ( 2D2 ) had no effect . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
In most of the patients , the presence of small amounts of either GPIIb or GPIIIa was detected in their platelets . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
On everted rabbit subendothelium platelet , adhesion was abnormal in BSS whichever shear stress and at high shear stress in vWD . ( 4 ) The molecular biology era ( 1984 1995 ) with the exquisite recognition of gene or protein anomalies in the above mentioned disorders together with the cloning of the 3 genes [ for vW factor ( vWF ) ( GPIb 9 , GPIIb , and GPIIIa ] . ( 5 ) We are at the dawn of the more crucial era , the antithrombotic therapy acting either on the GPIb vWF complex or on the GPIIb IIIa . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
In an immunoblot assay using polyclonal anti GpIIb IIIa antibodies , some GPIIIa ( 15 % of normal amount ) could be detected in the patient ' s platelets , whereas GPIIb could not ( < 2 % of normal amount ) . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Interestingly , several adhesion molecules expressed on platelets and endothelium also localize to chromosome 17 : the GP1BA locus ( glycoprotein ( GP ) Ibalpha ) has been provisionally mapped to the region 17p12 pter , the ITGA2B ( GPIIb ) and the ITGB 3 ( GPIIIa ) loci have been confirmed to the region 17q21 . 32 ; and the ICAM 2 locus has been provisionally mapped to the region 17q23 q 25 . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Purified talin was found to bind to both synthetic peptides corresponding to the cytoplasmic sequences of GPIIb ( P2b ) and GPIIIa ( P3a ) . ^^^ Thus , these results demonstrate direct binding of GPIIb IIIa to talin and suggest a role of the cytoplasmic sequences of both GPIIb and GPIIIa in mediating this interaction . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Three peptides derived from platelet receptor glycoprotein alphaIIbBeta 3 ( GPIIb / IIIa ) have been identified recently as fibrinogen binding sequences : GPIIb 300 314 and 656 667 and GPIIIa 211 223 . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Like its F ( ab ) fragments , the monoclonal antibody CRC 54 , whose epitope is located in the N terminal part of the GPIIIa molecule , binds to no more than 10 % of GPIIb IIIa on the resting platelet surface . ^^^ The data obtained indicate that the GPIIb IIIa transition to the active state and its interaction with ligands induces conformational changes in the N terminal part of GPIIIa and that the CRC 54 binding to the N terminal part of GPIIIa stimulates conformational changes in GPIIb IIIa , complex interaction with ligands and platelet aggregation . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Immunoblotting of platelet lysates demonstrated decreased levels of GPIIb ( approximately 30 % to 35 % of normal ) and GPIIIa ( approximately 10 % of normal ) , and the GPIIb had undergone normal maturational processing into GPIIb heavy and light chains . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Although the mRNA sequences for GPIIb and GPIIIa are identical in platelets and HEL cells , cell specific differences in the conformation states of the GPIIb IIIa complex may exist and may explain in part the contrasting functional properties . ^^^ Two monoclonal antibodies ( mAbs ) , an anti GPIIb mAb C 3 and an anti GPIIIa mAb D 3 , were used to determine whether differences in GPIIb IIIa conformational states could be measured . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Cotransfection of an expression vector containing the mutant GPIIb and wild type GPIIIa showed that the mutant Ser 870 > stop GPIIb was able to associate to GPIIIa . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
To examine how this mutation might alter the structure of GPIIb , G 273 was replaced by other amino acids and the resulting mutants were coexpressed with GPIIIa in COS 1 cells . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
While encountered only rarely , carriers of either GT or Bernard Soulier syndrome that are at the same time heterozygous for human platelet alloantigenic epitopes found on GPIb , GPIIb , or GPIIIa have the possibility to give discrepant results when comparing genotypic versus phenotypic analysis . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Previously we reported that circulating immune complexes ( CIC ) inhibited fibrinogen binding to platelet glycoprotein IIb / IIIa complex ( GPIIb / IIIa ) and that isolated Fc gamma R were recognized by monoclonal antibodies ( mAb ' s ) to GPIIb and GPIIIa ( J . ^^^ The Fc gamma R bound to immobilized immune complex were recognized by mAb ' s to GPIIb , GPIIIa , GPIIb / IIIa . ^^^ The dissociation of GPIIb / IIIa complex by incubating platelet extract at 37 degrees C in the presence of EDTA caused a marked decrease in the binding of GPIIb and GPIIIa to the immobilized immune complex . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
As judged by complex dependent monoclonal antibody ( MoAb ) binding , surface expression of platelet GPIIb / IIIa receptors was less than 5 . 5 % of normal , whereas the binding of an anti GPIIIa specific MoAb ( 7H2 ) was approximately 12 % of normal . ^^^ Immunoblot analysis of the patient ' s platelet lysates showed approximately 35 % of normal levels of GPIIIa , approximately 30 % of normal levels of GPIIb , and an abnormally migrating fragment of GPIIb . ^^^ Biotinylation of the surface proteins on the patient ' s platelets followed by immunoprecipitation and sodium dodecyl sulfate polyacrylamide gel electrophoresis ( SDS PAGE ) analysis showed only GPIIb and GPIIIa subunits of normal size . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
GT patients with mutations in the GPIIIa gene that cause diminished synthesis of GPIIIa are deficient in both GPIIb / IIIa and VnR , whereas patients with mutations in the GPIIb gene are deficient in GPIIb / IIIa , yet express normal or increased VnR in their platelets . ^^^ The presence or absence of VnR in platelet membranes of GT patients has therefore been used for distinguishing between mutations in the GPIIb gene and mutations in the GPIIIa gene . ^^^ The transformed lymphocytes transcribed GPIIIa mRNA but not GPIIb mRNA and expressed VnR on their surface . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Among central Europeans polymorphisms of GPIIIa , GPIb , GPIIb , and GPIa named human platelet antigen 1 ( HPA 1 ) , 2 , 3 , and 5 are the clinically most relevant systems in which alloimmunization occurs . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Glanzmann thrombasthenia is an autosomal recessive bleeding disorder caused by mutations in the genes encoding platelet GPIIb or GPIIIa . ^^^ These markers included polymorphisms within the thyroid hormone receptor alpha 1 gene ( THRA 1 ) , the breast cancer gene ( BRCA 1 ) , GPIIb , GPIIIa , and an anonymous marker D17S579 . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
SDS PAGE with Western blotting revealed an absence of GPIIb but small amounts of normally migrating GPIIIa in his platelets . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
This was demonstrated by the increase in MK markers ( GPIIb , GPIIIa , GPIb alpha , GPIX and vWF ) , the appearance of cytoplasmic alpha granules , intracellular membranes resembling demarcation membranes which were immunologically labeled with an GPIIb / IIIa anti antibody , and a small percentage of polyploid cells ( 8N and 16N ) . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
The platelet GPIIb GPIIIa heterodimer ( integrin alphaIIbbeta 3 ) binds fibrinogen with high affinity in response to activation by agonists , leading to platelet aggregation and formation of a hemostatic plug . ^^^ Transfection of cDNA encoding [ Ala 327 ] GPIIb , [ Gln 327 ] GPIIb , or [ Phe 327 ] GPIIb into Chinese hamster ovary cells inherently expressing GPIIIa permitted surface exposure of GPIIb IIIa complexes , whereas [ Glu 327 ] GPIIb did not . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
The candidates that might form disulfide bonds with actin were identified by monoclonal antibody to be GpIIb and / or GpIIIa . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Truncation of glycoprotein ( GP ) IIIa ( 616 762 ) prevents complex formation with GPIIb : novel mutation in exon 11 of GPIIIa associated with thrombasthenia . ^^^ The following observations indicate that this mutation is responsible for the thrombasthenic phenotype of the proband . ( 1 ) We failed to detect mutations other than [ T 1846 ] GPIIIa in the coding region of both GPIIb and GPIIIa genes . ( 2 ) The G 1846 > T mutation was observed in either parent and a brother of the proband , but none of 100 unrelated individuals carried this defect . ( 3 ) Pulse chase and immunoprecipitation analysis of GPIIb IIIa complexes in cells transiently cotransfected with cDNAs encoding normal GPIIb and [ T 1846 ] GPIIIa showed neither maturation of GPIIb nor complex formation and surface exposure of GPIIb triangle upGPIIIa . ^^^ These observations indicate that the sequence from Glu 616 to Thr 762 in GPIIIa is essential for heterodimerization with GPIIb . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Polymerase chain reaction ( PCR ) single stranded conformational polymorphism analysis of genomic DNA showed no mutations in the patient ' s GPIIIa and two novel mutations in the GPIIb gene : one of them was a heterozygous splice junction mutation , a C > A transversion , at position +2 of the exon 5 intron 5 boundary [ IVS 5 ( +2 ) C > A ] inherited from the father . ^^^ The proband showed a platelet accumulation of proGPIIb and minute amounts of GPIIb and GPIIIa . ^^^ Moreover , transfection and immunoprecipitation analysis demonstrated that [ Arg 674 ] GPIIb is capable of forming a heterodimer complex with GPIIIa , but the rate of subunit maturation and the surface exposure of GPIIb IIIa are strongly reduced . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Platelet GPs GPIb , GPIIbIIIa , GPIIb , and GPIIIa were evaluated . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Analysis of platelet proteins by Western blotting revealed trace amounts of normally migrating GPIIb and equally small amounts of GPIIIa of slightly slower than normal migration . ^^^ Flow cytometry confirmed a much decreased binding to platelets of monoclonal antibodies to GPIIb , GPIIIa or GPIIb IIIa , and an antibody to the alphav subunit also showed decreased binding . ^^^ This amino acid substitution presumably changes the capacity for disulphide bond formation within the cysteine rich core region of GPIIIa and its study will provide new information on GPIIb IIIa and alphavbeta 3 structure and biosynthesis . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Some investigators report that the vast majority of platelet antigens are conformation dependent , being destroyed by treatment with EDTA ( separation of GPIIb and GPIIIa ) or denaturation with detergents . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Unlike GPIIIa , there is little information about the clinical significance of the GPIIb polymorphism . ^^^ These data indicate that unlike the HPA 1 polymorphism of GPIIIa , the HPA 3 polymorphism of GPIIb may not serve as a useful genetic marker for the risk assessment of patients treated with intracoronary stenting . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
The human platelet antigen ( HPA ) 1 and the HPA 3 are the most extensively studied polymorphisms of GPIIIa and GPIIb , respectively . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Expression of genes specific for the megakaryocytic lineage , GPIIb , PF 4 , mpl and GPIIIa , was detected by reverse transcriptase polymerase chain reaction ( RT PCR ) during differentiation of ES cells , and their relative time course was evaluated . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
As expected , co transfection of Chinese hamster ovary ( CHO ) cells with cDNAs encoding GPIIIa and ( 288delC ) GPIIb failed to enhance the surface exposure of GPIIIa . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
By immunoblot analysis , GPIIb and GPIIIa levels in platelet lysates and membranes were near normal . ^^^ Sequence analysis of GPIIb and GPIIIa cDNA , and platelet mRNA levels for both subunits , were normal . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Platelet membrane analysis showed a reduction in glycoprotein ( GP ) Ib , but normal content of GPIIb and GPIIIa . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
When analyzed by flow cytometry , the binding of certain mAbs specific for GPIIb and / or GPIIIa was reduced in the presence of beta2GPI but not of APC or annexin 5 , whereas the binding of anti GPIb or anti P selectin mAbs , or of soluble fibrinogen remained unchanged . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Coexpression of normal [ 674C ] GPIIb and mutant [ 674R ] GPIIb with normal GPIIIa produced a [ 674R ] GPIIb concentration dependent inhibition of surface exposure of GPIIb IIIa complexes in Chinese hamster ovary ( CHO ) cells , suggesting that [ 674R ] GPIIb interferes with the association and / or intracellular trafficking of normal subunits . ^^^ BiP was also found associated with [ 674R ] GPIIb IIIa heterodimers , but not with normal GPIIIa or normal heterodimers . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
There is evidence that fibrinogen itself binds to regions in the amino terminal portions of both GPIIb and GPIIIa and that the sites interacting with the fibrinogen gamma chain and with RGD containing peptides are spatially distinct . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
To determine the contribution of each of these alterations to the thrombasthenic phenotype , Chinese hamster ovary or baby hamster kidney cells were cotransfected with normal GPIIb complementary DNA ( cDNA ) and the following GPIIIa cDNAs : normal , cDNA bearing IJ 1 mutation , 2011T > A mutated cDNA predicting C655S ( single letter amino acid codes ) substitution , and 2019A > T mutated cDNA predicting Stop 657 . ^^^ Immunohistochemical staining and immunoprecipitation demonstrated that the elimination of amino acids 657 762 in IJ 1 and Stop 657 prevented intracellular GPIIb / IIIa complex formation , and differential immunofluorescence staining of GPIIIa and cellular organelles suggested that the truncated uncomplexed GPIIIa protein was retained in the endoplasmic reticulum . ^^^ Because the use of GPIIIa Stop 693 and normal GPIIb cDNAs yielded GPIIb / IIIa complex formation , though with lower efficiency , it is suggested that amino acids 657 692 of GPIIIa are essential for the intracellular association of GPIIb and GPIIIa . ( Blood . 2001 ; 98 : 1063 1069 ) . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
To localize their epitopes using antigen capture enzyme linked immunosorbent assay ( ELISA ) , the reactivity of 34 PA anti GPIIb IIIa antibodies was examined with recombinant GPIIb IIIa having a defect in ligand binding sites in either GPIIb or GPIIIa , and no major conformational change was induced : KO variant GPIIb IIIa was attributed to a 2 amino acid insertion between residues 160 and 161 in the W 3 4 1 loop in GPIIb , and CAM variant GPIIb IIIa was attributed to D119Y in GPIIIa . ^^^ The present data suggest that certain PA anti GPIIb IIIa autoantibodies recognize epitopes close to the ligand binding site in GPIIb , but not in GPIIIa . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
The sequences obtained were compared with known cDNA sequences for canine GPIIb and GPIIIa . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
METHODS : Epitopes on GPIIb / IIIa complex were labeled with McAbs to GPIIb ( SZ 22 ) and GPIIIa ( SZ 21 ) conjugated to either a donor fluorescein ( FITC labeled ) or an acceptor ( TR labeled ) chromophore . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Using computer modelling , we divided the promoters , coding sequences and flanking splicing regions , in 31 segments for the GPIIb gene and 19 domains for the GPIIIa gene . ^^^ In the other patients , 10 novel mutations were characterised , which were located either in the GPIIb gene ( nine cases ) or in the GPIIIa gene ( one case ) . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Blood platelet activity was also evaluated as well as the expression of platelet glycoproteins ( GPIIb , GPIIIa , and GPIX ) in platelets of the patients and the controls . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
All alleles of GPIIb , GPIIIa , VWF , beta actin , hsp 70 , c mpl , Fli 1 , and FOG 1 genes are transcriptionally active in megakaryocytes from 4N to 32N . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Whether or not contacts between calf 1 and calf 2 domains of glycoprotein ( GP ) IIb ( alphaIIb ) and GPIIIa ( beta 3 ) play a role in GPIIb / IIIa complex formation has not been established . ^^^ Mutated GPIIb cDNAs were cotransfected in baby hamster kidney cells with normal GPIIIa ( beta 3 ) cDNA . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
This platelet aggregation activity was dose dependently inhibited by specific GPIbalpha antibodies , but not by those antibodies against platelet GPIa , GPIIa , GPIIb and GPIIIa . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
No other mutation than [ G188A ] GPIIb was found in the proband and her parents after complete analysis of GPIIb and GPIIIa coding sequences , and the promoter , 3 ' UTR , and intronic flanking regions of GPIIb . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Mutant GPIIb IIIa complexes were not detected on the surfaces of cells cotransfected with cDNAs of mutant GPIIb and normal GPIIIa . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Flow cytometric analysis showed that TMVA did not bind to platelet GPIX , GPIIb , GPIIIa , GPIa , GPIIa and GPIV . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
However , loss of GPIIb on BMMC results in an increase in surface expression of aV integrin , the alternative partner of GPIIIa . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
When a discrepancy was detected , genomic DNA from the mother was amplified for coding sequences of either the GPIIb or GPIIIa genes . ^^^ DNA sequence analysis revealed for Mother L a T ( 1447 ) > C a point mutation within exon 10 of the GPIIIa gene and for Mother S a C ( 480 ) > G point mutation within exon 4 of the GPIIb gene . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
A novel homozygous mutation ( 1619delC ) in GPIIb gene associated with Glanzmann thrombasthenia , the decay of GPIIb mRNA and the synthesis of a truncated GPIIb unable to form complex with GPIIIa . ^^^ Neither GPIIb nor its truncated form could be detected in the platelets of the patient by Western blotting , while a small amount of GPIIIa was demonstrated . ^^^ BHK cells transfected with wild type GPIIIa andmutated GPIIb failed to express any mature GPIIb or pro GPIIb . ^^^ Immunoprecipitation with a polyclonal antibody recognizing both GPIIb and GPIIIa recovered a 60 kDa truncated form of GPIIb . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
For example , microparticles in these experiments were found to contain membrane surface proteins including GPIIIa , GPIIb , and P selectin , as well other platelet proteins such as the chemokines CXCL 4 , CXCL 7 , and CCL 5 . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
The seven polymorphisms analyzed were chosen from six candidate genes involved in ( 1 ) Renin Angiotensin system : Angiotensin converting enzyme ( ACE I / D ) , ( 2 ) Lipid metabolism : Cholesterol Ester Transfer Protein gene ( CETP TAQ1B ) , ( 3 ) Platelet aggregation : alpha and beta subunits of the platelet GpIIb / GpIIIa integrin complex ( GpIIb HPA 3 and GpIIIa Pl ( A1 / A2 ) ) , ( 4 ) Coagulation fibrinolysis : Plasminogen Activator Tissue ( PLAT TPA 25 I / D ) and Methylenetetrahydrofolate Reductase ( MTHFR C677T and A1298C ) . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
The expression of glycoprotein ( GP ) Ib , GPIIb and GPIIIa on the surface of the patient ' s platelets was normal , and her platelet aggregation in response to ADP and ristocetin was also normal . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Conservation of the linkage of the murine Itga2b and Itgb 3 loci in mouse chromosome 11 . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
The critical segment on human chromosome 17 spans a large interval containing the genes Itga2b , Gfap , and Itgb 3 . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
The major haplotypes of seven other candidate genes , GP1BA , ITGA2B , ITGB 3 , GP 6 , VWF , FGB , and IL 6 , were not associated with bleeding severity . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Mobilized CD34+ cells contained subsets expressing CD 33 , CD 13 , CD45RA , CD 38 , HLA DR , CD 61 and CD 41 . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
MKPL 1 cells were positive for platelet GPIIb / IIIa ( CD 41 ) and GPIIIa ( CD 61 ) , and expressed OKM 5 ( CD 36 ) , MY 7 ( CD 13 ) , and MY 9 ( CD 33 ) antigens in the absence of erythroid and lymphoid markers . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
The immunophenotype ( a ) , ultrastructural features ( b ) and cell kinetics ( c ) of circulating megakaryoblasts have been studied in two cases of pure megakaryoblastic and one case of mixed ( myeloblastic , megakaryoblastic ) cell proliferation in chronic myeloid leukaemia ( CML ) . ( a ) The blast cells showed early megakaryocyte differentiation antigen ( HLA DR ) , platelet specific GpIIIa ( CD 61 ) and GpIIb IIIa ( CD 41 ) antigens in different percentages . ( b ) The megakaryoblasts were recognized by the presence of platelet GpIIIa ( CD 61 ) demonstrated by an immunoelectron microscopic method . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
In three cases , platelet antibodies Edu 3 ( CD 41 ) and GPIIIa ( CD 61 ) reacted with about 30 % of blast cells . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
The most intense and consistent degree of reactivity was seen using MAB to CD 9 , CD 13 , CD 29 , CD 31 , CD 44 , CD 46 , CD 51 , CD 54 and CD 55 , although reactivity was also observed using MAB belonging to CD 41 , CD 47 , CD 49 , CD 61 and CD 71 , in addition to a number of non clustered MAB . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Only a small proportion of CD34+ cells coexpressed CD 4 ( 15 . 5 + / 11 . 7 % , CD 36 ( 31 . 7 + / 6 . 2 % ) , CD 61 ( 16 . 3 + / 12 . 9 % ) , CD 41 ( 6 . 5 + / 5 . 5 % ) or the lymphoid associated markers CD 10 ( 18 . 6 + / 11 . 8 % ) and CD 19 ( 12 . 3 + / 13 . 2 % ) . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
The megakaryocytic nature of the leukemia has to be proven by ultrastructural demonstration of platelet peroxidase or by immunological demonstration of CD 61 , CD 42 , CD 41 on the surface of the leukemic blasts . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
A majority of cells had a blast morphology with the phenotype of a myeloid hematopoietic progenitor , ie , CD34+ , CD33+ , CD13+ , HLA DR+ , but CD 38 , and the remaining cells ( 5 % to 10 % ) expressed platelet restricted proteins such as CD 41 , CD 42 , CD 36 , CD 61 , and von Willebrand factor ; some of them were polyploid ( up to 32N ) and exhibited demarcation membranes and alpha granules . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Immunohistochemical staining after exposure to PMA showed that the expression of the platelet associated markers CD 41 and CD 61 was enhanced in all cell lines indicating possible differentiation along the megakaryocyte lineage . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
We describe a Down ' s neonate presenting with hyperleucocytosis and circulating blasts which were positive for CD 34 , myeloid ( CD 33 ) , megakaryocytic ( CD 41 , CD42b , CD 61 ) , and T lineage ( CD 3 , CD 7 ) , but not B lineage , associated antigens . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
The blasts expressed myeloid associated antigens CD 34 , CD 33 , and CD 13 , whereas the micromegakaryocytes were positive for CD 41 , CD42b , and CD 61 . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
They also compare the density of integrins ( CD 29 , CD49e , CD 41 , CD 51 and CD 61 ) and adhesive proteins from the group of `` homing ' ' receptors ( CD 44 ) and IgG `` superfamily ' ' ( LFA 1 , LFA 3 , ICAM 1 , N CAM ) and their changes after a single oral dose of a mixture of proteolytic enzymes ( Wobe Mugos , Wobenzym , MUCOS Pharma , FRG ) . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
TPO induced differentiation of UT 7 Wtmpl cells , not parental UT 7 cells , along the megakaryocytic lineage , as evidenced by decreased proliferation , changes in cell morphology , and increased surface expression and mRNA levels of megakaryocytic markers CD 41 , CD 61 , and CD42b . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Due to the close relationship of erythroid and megakaryoblastic progenitor cells and to the existence of a probably common precursor cell giving rise to these two different cell lineages , many megakaryoblastic cell lines express erythroid markers ( e . g . , expression of hemoglobin or glycophorin A ) and conversely cell lines with a predominant erythroid profile might display megakaryoblastic features ( e . g . , platelets peroxidase or glycoproteins CD 41 , CD42b or CD 61 ) . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
MPL upregulation triggered by PMA was accompanied by signs of induced differentiation such as increase in CD 41 , CD 42 and CD 61 expression , increase in cell size and cessation of proliferation . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Flow cytometric analysis of the GM CSF and TPO cultured lines displayed an up regulation of the megakaryocytic surface markers CD 41 , CD 42 and CD 61 , and a down regulation of the erythroid marker glycophorin A in the latter cell lines , suggesting some differentiation along the megakaryocytic lineage . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
MATERIAL AND METHODS : Mononuclear cells of 17 patients with CML in BC were immunophenotyped employing a panel of 18 monoclonal antibodies : CD 5 , CD 10 , CD 14 , CD 22 , and anti HLA DR used in all patients ; CD 2 , CD 15 , CD 19 , CD 34 , and CD 41 in 13 to 16 patients ; and CD 3 , CD 7 , CD 13 , CD 20 , CD 21 , CD 33 , CD42b , and CD 61 in less than 10 patients . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Contact cocultures generated cells expressing the CD 61 or CD 41 antigens . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Modifications in the presence of platelet glycoproteins GPIb ( CD42b ) , GPIIIa ( CD 41 ) and GPIV ( CD 36 ) , expression of specific platelet markers ( P selectin ( CD62P ) and lysosomal protein ( CD 63 ) ) and leukocyte integrin ( CD11b ) were assessed during hemodialysis . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Megakaryocytic cells were monitored by detecting the expression of GPIIIa ( CD 61 ) , GPIIb / IIIa ( CD 41 ) and GPIb ( CD42b ) , and the distribution of megakaryocyte ( MK ) ploidy was analyzed by two color flow cytometry . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
RESULTS : The addition of TPO and SCF alone generated a population positive for the antigens CD 41 ( 5 . 5+ / 2 . 9 % ) and CD 61 ( 6 . 1+ / 2 . 2 % ) but induced a low amplification of cell number ( 8 . 1+ / 0 . 9 fold expansion ) . ^^^ The presence of IL 6 or IL 11 was associated with MK progenitor cell expansion , and up to 7 10 % of cultured cells were found to be CD 41 and CD 61 positive by flow cytometry . ^^^ Conversely , the addition of IL 3 to this cytokine combination was associated with a prominent expansion of the myeloid lineage ( 70+ / 10 % of CD33+ cells ) but only 0 . 9 % and 2 % of cultured cells were positive for CD 61 and CD 41 respectively . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
The majority of CD 61 ( + ) cells generated in TPO or PMP based medium were low ploidy 4N and 8N cells , and ex vivo generated CD 61 ( + ) , CD 41 ( + ) , and CD42b ( + ) cells were mainly double positive for FACS measured intracellular von Willebrand Factor ( vWF ) ( 76 . 7 + / 3 . 3 % , 58 . 8 + / 4 . 4 % , and 82 . 7 + / 2 . 5 % , respectively ) . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
A three color flow cytometric analysis was performed and revealed two different CD 45 dim , CD 34 ( + ) , CD 117 ( + ) , CD 56 ( + ) immature subpopulations : the normal immature myeloid precursor and an immature blast cell population that expressed CD 41 , CD 42 , CD 61 , CD 36 , CD 13 , CD1a , and CD 2 . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
These assay conditions were also well suited for assessment of platelet expression of CD 41 , CD42a , CD 61 and CD 63 . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
A clear and unambiguous criteria for the identification of megakaryocytic cells is described based on the high expression of platelet glycoprotein ( e . g . , CD 61 ( hi ) or CD 41 ( hi ) ) but not the monocyte marker ( CD 14 ( neg ) ) . . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Most ( > 75 % ) agreed that at least one more B ( CD 20 , CD 22 , CD79a , IgM ) , T ( CD1a , CD 2 , CD 4 , CD 5 , CD 8 ) , myeloid ( CD11b , CD 15 , CD 64 , CD 117 , myeloperoxidase ) , erythroid ( CD 36 , CD 71 , glycophorin A ) , and megakaryocytic ( CD 41 , CD 61 ) reagents should be included in the essential panel . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Two antibodies ( CD 61 and CD 41 ) were used for identifying platelets and individually gave acceptable results , but in a minority of samples , staining differences were observed . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Similar studies were performed using liquid culture medium , and after 14 days the number of MNCs , CD 34 ( + ) , AC 133 ( + ) , CD 41 ( + ) , and CD 61 ( + ) cells were higher in the UCB cells cultured in IL 3 , TPO , SCF , and Flt 3 compared to those cultured with those four cytokines plus GM CSF . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
MLSC was able to measure antigens CD 9 , CD 29 , CD 36 , CD 41 , CD42a , CD42b , and CD 61 on platelets and determine an average of 2 . 3 10 10 ( 5 ) + / 7 10 10 ( 4 ) platelets per microliter . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Blasts in all expressed CD 45 , CD 38 , and CD 33 ; most AMLs and all TMDs were CD36+ ; and the majority expressed CD 41 and CD 61 , suggesting megakaryocytic differentiation . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Megakaryocytic cells isolated from these cultures ( 1 ) express several markers characteristic of this lineage ( CD 41 , CD 61 , CD 62 P , CXCR 4 , PAR 1 , etc . ) , ( 2 ) respond by calcium flux and phosphorylation of various intracellular proteins to stimulation by thrombin and ( 3 ) adhere to fibrinogen and vitronectin . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
As a result of this interaction , microparticles derived from platelets ( PMPs ) may transfer many platelet antigens ( CD 41 , CD 61 , CD 62 , CXCR 4 , PAR 1 ) to the surfaces of HSPCs . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Immunoprecipitation and western blot analysis confirmed that CD 41 was present in cells from embryoid bodies associated with CD 61 ( beta 3 integrin , platelet GPIIIa ) in a complex . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
CD 61 is a membrane glycoprotein that associates with CD 41 ( alphaIIb ) to form the heterodimeric complex gpIIb / IIIa ( CD41 / CD61 ) , predominantly expressed in platelets and megakariocytes . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
RESULTS : We found that , in addition to platelet endothelium attachment receptors ( CD 41 , CD 61 and CD 62 ) , PMPs express G protein coupled seven transmembrane span receptors such as CXCR 4 and PAR 1 ; cytokine receptors including TNF RI , TNF RII , and CD 95 ; and ligands such as CD40L and PF 4 . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Platelet glycoproteins CD 41 , CD 42a , C 42b , CD 61 , and PAC 1 were also assayed and found to be normally expressed . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Cytochemical stains were all negative , immunophenotyping studies showed CD 41 and CD 61 positivity in blast cells . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
In addition , megakaryocyte associated antigens CD 41 and CD 61 were found to be positive . ^^^ MOLM 16 revealed characteristics of the megakaryocytic lineage in terms of immunophenotyping being positive for CD 9 , CD 31 , CD 36 , CD 41 , CD 61 , CD62P , CD 63 , CD 110 , CD 151 , thrombospondin , von Willebrand factor ( vWf ) , and fibrinogen . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
MK lineage cell expansion was assessed using mononuclear cell count and flow cytometry ( CD34 / 41 , CD 41 , CD34 / 61 , and CD 61 expression ) on days 7 , 11 , and 14 . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
CD 41 ( bright ) yolk sac definitive progenitor cells co express CD 61 and bind fibrinogen , demonstrating receptor function . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Flow cytometry revealed variable GPIIb / IIIa expression by CD 61 and CD 41 in patients with Glanzmann thrombasthenia on the basis of CD 61 levels , six patients were subtyped as type 1 because they had absent GPIIb / IIIa , three patients were subtyped as type 2 because their GPIIb / IIIa levels varied from 7 . 72 % to 20 . 40 % , and one patient was diagnosed as type 3 , because his clot retraction was 60 % and GPIIb / IIIa was 46 . 0 % of normal . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Down regulation of NDR correlated well with up regulation of megakaryocytic markers , CD 41 and CD 61 . ^^^ Overexpression of the nuclear NDR GFP in K 562 cells inhibited the expression of CD 41 and CD 61 in megakaryocytic differentiation . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
JTZ 132 increased the number of megakaryocyte specific marker , CD 61 ( + ) and CD 41 ( + ) , cells in cultures of mouse and human bone marrow cells , respectively , and the colony forming unit megakaryocytes in mouse bone marrow cells . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Cells were immunolocalised for CD 61 , CD 41 , ER alpha and beta . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
The platelet associated immunoglobulin ( PAIg ) and platelet membrane glycoprotein ( CD 41 , CD 61 , GPIIb / IIIa ) in 76 cases of immune thrombocytopenia and 30 healthy subjects were determined by FCM . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Megakaryocytic lineage was established by the presence of one or more megakaryocyte / platelet associated antigens ( CD 41 , CD42b , and CD 61 ) in > 20 % blast cells . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
During this interval , CD 41 and CD 61 expression on the cell surface increased . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Flow cytometric quantitation of CD 41 , CD42a , CD42b and CD 61 was carried out using a Becton Dickinson FACScan . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Immunophenotyping of the first leukemia sample revealed a mixed lineage leukemia immunophenotype with positivity for terminal deoxynucleotidyl transferase ( TdT ) , CD 13 and CD 19 ; the second sample revealed solely myeloid characteristics with positivity for CD 13 , CD 41 and CD 61 , whereas TdT was negative . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Cell sorter isolation and single cell cloning experiments indicated that the small cells were those capable to proliferate in TPO and to generate the larger ones over time . 32D TPO cells expressed Mk specific markers by FACS ( CD 41 , CD 61 and 2D5 ) and RT PCR ( acetyl cholinesterase E and platelet factor 4 ) and their unique profile , by gene array analysis , included expression of urokinase plasminogen activator surface receptor ( CD 87 or uPAR ) , plasminogen activator inhibitor and coagulation factor 2 ( thrombin ) receptor ( Cf2r ) . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
To determine the cellular mechanism by which oestrogen influences megakaryocytopoesis we also examined its effect on markers of megakaryocytic differentiation ( CD 41 , CD 61 , CD 34 ) . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
When highly purified human CD34+ cells were cultured for 7 days with TPO alone , the generated cells expressed megakaryocytic markers , such as CD 41 , CD42b , and CD 61 . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
This study was undertaken to examine the tissue distribution of a broad spectrum of monoclonal antibodies ( mAbs ) containing tetraspan antigens ( CD 9 , CD 63 , CD 151 ) , endothelial cell antigens ( CD 31 , CD 36 , CD 105 , CD 106 , CD 146 ) , integrins ( CD49a f , CD 29 , CD 41 , CD 51 , CD 61 ) , CD 39 , CD 98 , CD 99 , CD 143 and , CD 147 supplied from fifth and sixth international workshops and conferences on human leukocyte differentiation antigens in a comparative manner in human OA and normal synovium . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
RESULTS : Immunophenotyping showed that the JAS R cells were positive for CD 33 , CD 41 and CD 61 , as well as moderately to weakly positive for CD 4 , CD 7 , CD 13 and glycophorin A . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
An extensive series of Mab ( CD 2 , CD 3 , CD 4 , CD 8 , CD11b , CD 13 , CD 14 , CD 16 , CD 19 , CD 22 , CD 33 , CD 34 , CD 41 , CD42b , CD 45 , CD 56 , CD 61 , CD 64 , CD235a and HLA DR ) were tested singly or in FITC / PE combinations . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Flow cytometry , immunoblot analysis , and RT PCR demonstrated that VPA induced the expression of the early MK markers GpIIIa ( CD 61 ) and GpIIb / IIIa ( CD 41 ) in a dose dependent manner . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
MATERIAL AND METHODS : In age and sex matched 19 healthy subjects , 20 hemodialysis ( HD ) patients , and 18 continuous ambulatory peritoneal dialysis ( CAPD ) patients , the expression of platelet surface receptors CD 41 , CD 61 , CD42a , and CD62P were investigated . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
The demonstration of antiplatelet antibodies ( PAIgG , PAIgM ) and decreased detection of platelet surface antigens ( CD 41 , CD 61 , CD42b ) in children with immune thrombocytopenic purpura ( ITP ) have a diagnostic role . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Using the cDNA PCR it was seen that the mRNA phenotype of a small number of sorted CD34+ cells ( purity > 98 % ) was negative for the markers CD 2 , CD 14 , CD 16 , CD 20 , CD 21 , CD 22 , CD41b , and glycophorin A that are expressed on differentiated cells but positive for CD 34 , CD 7 , CD 19 , CD 36 , and CD 61 . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Some reactivity with mAbs against the platelet antigens CD41b ( IIb ) and CD 61 ( beta 3 ) was detected . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Receptor expression was measured by flow cytometry with monoclonal murine anti human antibodies CD 9 ( p 24 ) , CD42B ( Ib ) , CD41b ( IIb ) , CD 61 ( IIIa ) , CD41a ( IIb / IIIa ) , CD49b ( VLA 2 ) , CD62p ( P selectin ) , CD 31 ( PECAM 1 ) , and CD51 / CD61 ( vitronectin ) . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Receptor expression was measured by flow cytometry with monoclonal antibodies to p 24 ( CD 9 ) , Ib ( CD42b ) , IIb ( CD41b ) , IIIa ( CD 61 ) , IIb / IIIa ( CD41b / CD61 ) , very late antigen 2 ( CD49b ) , P selectin ( CD62p ) , platelet / endothelial cell adhesion molecule 1 ( CD 31 ) ; and vitronectin ( CD51 / CD61 ) . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Adsorption experiments demonstrated that pGlcNAc fibers tightly bind most major plasma proteins and a specific sub set of platelet surface proteins , including the integrin beta ( 3 ) subunit ( CD 61 ) and the von Willebrand receptor GP1b ( CD42b ) . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
Genetic analysis showed that GTa was a compound heterozygote for the GPIIIa gene . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
In addition , significant interactions of HPA 3 with the Pl ( A ) polymorphism of GPIIIa ( P=0 . 002 ) and with fibrinogen ( P=0 . 01 ) were identified in relation to mortality . ^^^
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA
Interacting proteins: P08514 and P05106	Pubmed	SVM Score :0.0
NA