| Interacting proteins: P04792 and P99999 |
Pubmed |
SVM Score :0.0 |
| Hsp 27 negatively regulates cell death by interacting with cytochrome c . ^^^ We show that the small HSP known as Hsp 27 inhibits cytochrome c mediated activation of caspases in the cytosol . ^^^ Hsp 27 binds to cytochrome c released from the mitochondria to the cytosol and prevents cytochrome c mediated interaction of Apaf 1 with procaspase 9 . ^^^ |
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| Interacting proteins: P04792 and P99999 |
Pubmed |
SVM Score :0.0 |
| In thermotolerant cells , Hsp 27 was shown to associate with the mitochondrial fraction , and inhibition of Hsp 27 induction during thermotolerance in cells transfected with hsp 27 antisense potentiated mitochondrial cytochrome c release after exposure to various apoptotic stimuli , despite the presence of elevated levels of Hsp 72 . ^^^ In vitro studies revealed that recombinant Hsp 72 more efficiently blocked cytochrome c mediated caspase activation than did recombinant Hsp 27 . ^^^ A model is presented for the inhibition of apoptosis during thermotolerance in which Hsp 27 preferentially blocks mitochondrial cytochrome c release , whereas Hsp 72 interferes with apoptosomal caspase activation . . ^^^ |
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| Interacting proteins: P04792 and P99999 |
Pubmed |
SVM Score :0.0 |
| HSP 27 inhibits cytochrome c dependent activation of procaspase 9 . ^^^ In contrast with Bcl 2 , HSP 27 overexpression does not prevent etoposide induced cytochrome c release from the mitochondria . ^^^ In a cell free system , addition of cytochrome c and dATP to cytosolic extracts from untreated cells induces the proteolytic activation of procaspase 3 in both control and bcl 2 transfected U 937 cells but fails to activate procaspase 3 in HSP 27 overexpressing cells . ^^^ Immunodepletion of HSP 27 from cytosolic extracts increases cytochrome c / dATP mediated activation of procaspase 3 . ^^^ In the cell free system , immunodepletion of HSP 27 increases LEDH AFC peptide cleavage activity triggered by cytochrome c / dATP treatment . ^^^ |
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| Interacting proteins: P04792 and P99999 |
Pubmed |
SVM Score :0.0 |
| Hsp 27 functions as a negative regulator of cytochrome c dependent activation of procaspase 3 . ^^^ Here we demonstrate that heat shock protein 27 ( Hsp 27 ) inhibits cytochrome c ( cyt c ) dependent activation of cas 3 . ^^^ |
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| Interacting proteins: P04792 and P99999 |
Pubmed |
SVM Score :0.0 |
| Hsp 27 as a negative regulator of cytochrome C release . ^^^ We previously showed that Hsp 27 protects against apoptosis through its interaction with cytosolic cytochrome c . ^^^ We report that Hsp 27 also interferes , in a manner dependent on level of expression , with the release of cytochrome c from mitochondria . ^^^ Moreover , a decreased level of endogenous Hsp 27 , which sensitized HeLa cells to apoptosis , reduced the delay required for cytochrome c release and procaspase 3 activation . ^^^ Moreover , we show that only a very small fraction of cytochrome c interacts with Hsp 27 , hence excluding a role of this interaction in the retention of cytochrome c in mitochondria . ^^^ |
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| Interacting proteins: P04792 and P99999 |
Pubmed |
SVM Score :0.0 |
| We tested the hypotheses that HSP 27 may reduce poly ( Q ) mediated cell death either by binding cytochrome c and inhibiting the mitochondrial death pathway or by protecting against reactive oxygen species ( ROS ) . ^^^ While poly ( Q ) induced cell death was reduced by inhibiting cytochrome c ( cyt c ) release from mitochondria , protection by HSP 27 was regulated by its phosphorylation status and was independent of its ability to bind to cyt c . ^^^ |
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| Interacting proteins: P04792 and P99999 |
Pubmed |
SVM Score :0.0 |
| Taken together , these findings suggest that apoptosis in HSG cells induced by concanamycin A is regulated by cytochrome c released from mitochondria into cytoplasm and the subsequent activation of caspases , and that HSP 27 may interfere with caspase dependent apoptotic cell death induced by concanamycin A . . ^^^ |
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| Interacting proteins: P04792 and P99999 |
Pubmed |
SVM Score :0.0 |
| In the present study , we exposed to NAMI A the spontaneously transformed human endothelial cell line ECV 304 and assessed a number of apoptosis related features , including the DNA degradation rate , the activation of caspase 3 protease , the expression of Hsp 27 , and the release of cytochrome c . ^^^ Furthermore , NAMI A was able to down regulate Hsp 27 protein expression and provoke the release of mitochondrial cytochrome c in the cytosol . ^^^ |
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| Interacting proteins: P04792 and P99999 |
Pubmed |
SVM Score :0.0 |
| Hsp 27 ' s neuroprotective action is downstream of cytochrome c release from mitochondria and upstream of caspase 3 activation . ^^^ |
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| Interacting proteins: P04792 and P99999 |
Pubmed |
SVM Score :0.0 |
| Cell free 27 kDa heat shock protein ( hsp 27 ) and hsp 27 cytochrome c complexes in the cervix of women with ovarian or endometrial cancer . ^^^ We now report the detection of hsp 27 as well as hsp 27 cytochrome c complexes in cell free endocervical or posterior vaginal specimens from women with endometrial or ovarian cancer . ^^^ After removal of cellular components , aliquots of supernatants were assayed by ELISA for hsp 27 , using cytochrome c bound to microtiter plate wells , and for hsp 27 cytochrome c complexes , using antibodies to cytochrome c and hsp 27 . ^^^ Among 47 women with ovarian cancer , 38 . 3 % were positive for hsp 27 and 27 . 7 % had hsp 27 cytochrome c complexes . ^^^ Similarly , among 52 women with endometrial cancer , 34 . 6 % were hsp 27 positive and 30 . 8 % had hsp 27 cytochrome c complexes . ^^^ |
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| Interacting proteins: P04792 and P99999 |
Pubmed |
SVM Score :0.0 |
| The p 38 MAPK inhibitor SB 203580 abrogated activation of p 38 MAPK , translocation of HSP 27 , and F actin reorganization and prevented cytochrome c release , caspase 3 activation , and DNA fragmentation . ^^^ |
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| Interacting proteins: P04792 and P99999 |
Pubmed |
SVM Score :0.0 |
| Bax and cytosolic cytochrome c increased , and the mitochondrial transmembrane potential and Hsp 25 expression was reduced in vitamin E deficiency . ^^^ |
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| Interacting proteins: P04792 and P99999 |
Pubmed |
SVM Score :0.0 |
| Heat shock protein 25 ( HSP 25 ) interferes negatively with apoptosis through several pathways that involve its direct interaction with cytochrome c or Akt . ^^^ |
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| Interacting proteins: P04792 and P99999 |
Pubmed |
SVM Score :0.0 |
| FK 506 , but not 5 10 , 367 , inhibited the cytochrome c release out of the mitochondria and the caspase 3 activation , while both molecules inhibited the cleavage of Poly ( ADP ribose ) polymerase ( Parp ) and prevented the expression of p 53 . ( 3 ) FK 506 and 5 10 , 367 rapidly induced the expression of Hsp 70 and Hsp 27 , but not Hsp 90 . ^^^ |
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| Interacting proteins: P04792 and P99999 |
Pubmed |
SVM Score :0.0 |
| Heat shock protein 27 ( Hsp 27 ) negatively regulates another mitochondrial protein , cytochrome c , during apoptosis ; however , the role of Hsp 27 in modulating Smac release is unknown . ^^^ |
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| Interacting proteins: P04792 and P99999 |
Pubmed |
SVM Score :0.0 |
| HSP 27 is an ATP independent chaperone that confers protection against apoptosis through various mechanisms , including a direct interaction with cytochrome c . ^^^ |
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| Interacting proteins: P04792 and P99999 |
Pubmed |
SVM Score :0.0 |
| Additional studies showed that MGO induced modification of Hsp 27 decreased its binding to cytochrome c . ^^^ |
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| Interacting proteins: P04792 and P99999 |
Pubmed |
SVM Score :0.0 |
| Hsp 27 inhibits 6 hydroxydopamine induced cytochrome c release and apoptosis in PC 12 cells . ^^^ Prior heat shock or overexpression of Hsp 27 ( human homologue of Hsp 25 ) delayed cytochrome c release , caspase activation , and reduced the level of apoptosis caused by 6 OHDA . ^^^ |
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| Interacting proteins: P04792 and P99999 |
Pubmed |
SVM Score :0.0 |
| The potentiation of As2O3 provoked apoptosis involved the increased disruption of mitochondrial transmembrane potential , increased caspase 3 activation and cytochrome c release from mitochondria , increased Bax and Bid activation , and attenuation of 27 kDa heat shock protein ( HSP 27 ) expression ; the potentiation was prevented by Bcl 2 overexpression . ^^^ |
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| Interacting proteins: P04792 and P99999 |
Pubmed |
SVM Score :0.0 |
| RESULTS : RNA interference for HSP 27 resulted in a twofold increase in VP 16 induced apoptosis , which was preceded by enhanced p 38 and c Jun phosphorylation and a twofold increased cytochrome c release into the cytoplasm . ^^^ CONCLUSION : 1 ) HSP 27 inhibits VP 16 mediated phosphorylation of p 38 and c Jun , cytochrome c release , and subsequent apoptosis ; 2 ) HSP 27 is expressed and activated in monocytic AML blasts ; 3 ) cytoplasmic expression of p 21 compensates for the lack of HSP27 . . ^^^ |
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| Interacting proteins: P04792 and P99999 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04792 and P99999 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04792 and P99999 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04792 and P99999 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04792 and P99999 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04792 and P99999 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04792 and P99999 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04792 and P99999 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04792 and P99999 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04792 and P99999 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04792 and P99999 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04792 and P99999 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04792 and P99999 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04792 and P99999 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04792 and P99999 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04792 and P99999 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04792 and P99999 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04792 and P99999 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04792 and P99999 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04792 and P99999 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04792 and P99999 |
Pubmed |
SVM Score :0.0 |
| NA |
|