Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
MAP kinase activated protein kinase 2 ( MAPKAP kinase 2 ) phosphorylates the serine residues in murine heat shock protein 25 ( hsp 25 ) and human heat shock protein 27 ( hsp 27 ) which are phosphorylated in vivo in response to growth factors and heat shock , namely Ser 15 and Ser 86 ( hsp 25 ) and Ser 15 , Ser 78 and Ser 82 ( hsp 27 ) . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
These findings demonstrate that the activation of p 38 MAP kinase is involved not only in response to stress , but also in physiological signaling by gastrointestinal hormones such as CCK , where activation of Gq coupled receptors stimulates a cascade in which p 38 MAP kinase activates MAP kinase activated protein kinase 2 , resulting in Hsp 27 phosphorylation . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
More recently , we identified MAP kinase activated protein kinase 2 ( MAPKAPK 2 ) and the 27 kDa heat shock protein ( HSP 27 ) as downstream regulators of p 38 MAP kinase . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
Role for p 38 mitogen activated protein kinase in platelet aggregation caused by collagen or a thromboxane analogue . p 38 mitogen activated protein kinase ( MAPK ) was identified in platelets on the basis of ( a ) its reactivity with antibodies to C terminal and N terminal peptides , and ( b ) its ability to activate MAPK activated protein kinase 2 , which phosphorylates the small heat shock protein , hsp 27 . p 38 MAPK was activated in platelets by collagen fibers , a collagen related cross linked peptide , thrombin , or the thromboxane analogue U 46619 . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
Finally , downstream of Rac and p 38 mitogen activated protein kinase ( MAPK ) , Gas6 / Ark signaling promoted activation of MAPK activated protein kinase 2 and induced phosphorylation of HSP 25 , a known regulator of cortical actin remodeling . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
We showed previously that Akt exists in a signaling complex with p 38 MAPK , MAPK activated protein kinase 2 ( MAPKAPK 2 ) , and heat shock protein 27 ( Hsp 27 ) ; and Hsp 27 dissociates from the complex upon neutrophil activation . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
P 38 MAPK interacts with the actin cytoskeleton via the MAPK activated protein kinase 2 ( MAPKAPK 2 ) and heat shock protein 27 ( HSP 27 ) . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
Analysis of properties of small heat shock protein Hsp 25 in MAPK activated protein kinase 2 ( MK 2 ) deficient cells : MK 2 dependent insolubilization of Hsp 25 oligomers correlates with susceptibility to stress . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
Similar to human HSP 27 , the canine protein contains three Ser residues that are potential MAPKAP kinase 2 substrates . . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
These results establish that MAPKAP kinase 2 is a physiological RK substrate , and that HSP 27 is phosphorylated by MAPKAP kinase 2 in vivo . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
MAPKAP kinase 2 activity was determined using the small heat shock proteins ( sHsps ) Hsp 25 and Hsp 27 as substrates . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
In those cells , however , Hsp 27 phosphorylation could be reconstituted when activated immunoprecipitated MAP kinase or purified MAPKAP kinase 2 was added . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
The capacity of cellular extracts to phosphorylate Hsp 27 could be , however , restored when either immunoprecipitated activated MAP kinase or purified MAPKAP kinase 2 was added to cell lysates . ^^^ These findings suggest that IL 6 mediated phosphorylation of Hsp 27 results from activation of MAPKAP kinase 2 , a serine / threonine kinase which is activated by MAP kinase . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
Stress and mitogen induced phosphorylation of the small heat shock protein Hsp 25 by MAPKAP kinase 2 is not essential for chaperone properties and cellular thermoresistance . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
Northern blot analysis demonstrates a 3 . 5 kb MAPKAP kinase 2 transcript which is ubiquitously expressed and , hence , co expressed with the mRNA of the recently identified substrate Hsp 25 in all tissues analysed . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
TNF Treatment of cell activated the p 38 MAP kinase pathway , as revealed by increased phosphorylation of p 38 MAP kinase itself , activation of the substrate protein MAPKAP kinase 2 , and culminating in the phosphorylation of the heat shock protein 27 ( hsp 27 ) . ^^^ Pretreatment of cells with the highly specific p 38 MAP kinase inhibitor SB 203580 completely blocked this TNF induced activation of MAPKAP kinase 2 and hsp 27 phosphorylation . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
Based on the MAPKAP kinase 2 phosphorylation site of Hsp 27 , a competitive inhibitory peptide was synthesized . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
MAPKAP kinase 3 and MAPKAP kinase 2 phosphorylated peptide substrates with similar kinetic constants and phosphorylated the same serine residues in HSP 27 at the same relative rates . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
Hypo osmotic swelling of human Intestine 407 cells leads to a significant increase of intracellular MAPKAP kinase 2 activity and Hsp 27 phosphorylation . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
The hsp 27 specific kinase MAPKAP kinase 2 was activated 2 . 4 fold with 1 nmol / L cholecystokinin treatment . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
As a result , PMA induced Hsp 27 phosphorylation is inhibited in SB 203580 treated HL 60 cells indicating that p38RK and MAPKAP kinase 2 are components of the PMA induced signal transduction pathway leading to Hsp 27 phosphorylation . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
In addition , in vitro protein phosphorylation analysis with recombinant MAPKAP kinase 2 showed that small heat shock protein ( hsp 25 ) served as a major substrate molecule for the kinase in H9c2 cells and the protein phosphorylation of cellular hsp 25 was stimulated by H2O2 induced oxidative stress or PMA treatment in intact H9c2 cells . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
SB 203580 specifically inhibits activation of p38RK as judged by MAPKAP kinase 2 activity against the substrate Hsp 27 and also blocks Hsp 27 phosphorylation in the cells . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
To determine whether muscarinic receptors are coupled to a `` stress response ' ' pathway in smooth muscle culminating in phosphorylation of HSP 27 , we assayed MAPKAP kinase 2 activity and tyrosine phosphorylation of p 38 mitogen activated protein ( MAP ) kinase , the enzyme thought to activate MAPKAP kinase 2 . ^^^ Recombinant canine HSP 27 expressed in Escherichia coli was a substrate for MAPKAP kinase 2 in vitro as well as a substrate for endogenous smooth muscle HSP 27 kinase , which was activated by carbachol . ^^^ We suggest that HSP 27 in airway smooth muscle , in addition to being a stress response protein , is phosphorylated by a receptor initiated signaling cascade involving muscarinic receptors , tyrosine phosphorylation of p 38 MAP kinase , and activation of MAPKAP kinase 2 . . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
Peak ' d ' contained a 43 kDa form . ' In gel ' kinase assays using hsp 27 indicated both forms of MAPKAP kinase 2 were active . ^^^ Both forms of hsp 27 kinase immunoprecipitated with a MAPKAP kinase 2 antibody and have therefore been named MAPKAP kinase 2alpha ( p 49 ) and MAPKAP kinase 2beta ( p 43 ) . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
Pretreatment of cells with the specific p 38 MAP kinase inhibitor SB 203580 completely blocked the ANG 2 dependent activation of MAPKAP kinase 2 and phosphorylation of HSP 27 . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
Interleukin 3 and granulocyte macrophage colony stimulating factor induce activation of the MAPKAP kinase 2 resulting in in vitro serine phosphorylation of the small heat shock protein ( Hsp 27 ) . ^^^ We show here that IL 3 and GM CSF induce MAPKAP kinase 2 activity in the human megakaryoblastic leukemia cell line MO 7 and phosphorylate the human small heat shock protein Hsp 27 on serine residues in vitro . ^^^ GM CSF also induced Hsp 27 phosphorylation in neutrophils in a range similar to that observed in MO 7 cells , suggesting that MAPKAP kinase 2 mediated Hsp 27 activation occurs independently of proliferation . ^^^ Taken together , our findings indicate that tyrosine phosphorylation of MAP kinase is a prerequisite for serine phosphorylation of Hsp 27 , which is mediated by MAPKAP kinase 2 . ^^^ Our data identify Hsp 27 as a target of the IL 3 / GM CSF stimulation pathway that involves MAP kinase and MAPKAP kinase 2 . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
Peak activation of p 38 , MAPKAP kinase 2 , and HSP 25 were sixfold at 5 min , sixfold at 5 min , and threefold at 30 min compared with static control , respectively . ^^^ SB 203580 ( 1 microM ) , a specific inhibitor of p 38 , abolished the activation of MAPKAP kinase 2 and HSP 25 as well as EC elongation and alignment in the direction of flow elicited by shear stress . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
To test the hypothesis that cGK could inhibit platelet aggregation by phosphorylating Hsp 27 and interfering with the MAPKAP kinase phosphorylation of Hsp 27 , the known MAPKAP kinase 2 phosphorylation sites ( Ser 15 , Ser 78 , and Ser 82 ) as well as Thr 143 were replaced by negatively charged amino acids , which are considered to mimic phosphate groups , and tested in actin polymerization experiments . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
Activation of P 38 SAPK leads to activation of MAPKAP kinase 2 and a major substrate protein of MAPKAP kinase 2 is the small heat shock protein , HSP 27 . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
Of relevance , 1alpha , 25 ( OH ) 2D3 induced in the C2C12 line the stimulation of mitogen activated protein kinase activating protein kinase 2 ( MAPKAP kinase 2 ) and subsequent phosphorylation of heat shock protein 27 ( HSP 27 ) in a p 38 kinase activation dependent manner . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
MonoQ fractionation of cell extracts resolved phosphotransferase activity peaks toward MBP , GST c Jun ( 1 169 ) , and hsp 27 , which contained MAPK , SAPK / JNK , and MAPKAPK 2 , respectively , as indicated by immunoblotting data . ^^^ PMA , which activated the MAPK as potently as LPS , did not strongly activate MAPKAPK 2 , as assessed by hsp 27 phosphorylation . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
Stress factors stimulate c Jun activation through a related kinase pathway involving Mekk > Sek > SAPK c Jun , and hsp 27 phosphorylation via the MKK 3 > Hog > MAPKAPK 2 hsp 27 route . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
Resource Q chromatography resolved two distinct peaks of hsp 27 phosphotransferase activities ; the first peak ( at 0 . 36 M NaCl ) appeared to correspond to the 55 kDa MAPKAPK 2 . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
The MAPKAPK 2 substrate hsp 27 may be involved in stabilization , as beta globin Cox 2 transcripts were partially stabilized by phosphomimetic mutant forms of hsp 27 . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
SB 202190 suppressed the kinase activity of p 38 , leading to inhibition of activation of MAPKAPK 2 and then the subsequent phosphorylation of hsp 27 in LPS treated macrophages both in vitro and in vivo , but an inactive analog of SB 202190 , SB 202474 , did not . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
Heat shock protein ( HSP 27 ) is a cytoskeleton interacting substrate of MAPKAPK 2 . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
Similarly , the small heat shock protein , Hsp 27 , which is phosphorylated by MAPKAPK 2 , a downstream substrate of p 38 , was phosphorylated in response to the addition of laminin to the cocultures . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
Since Hsp 27 is a downstream target of p 38 mitogen activated protein kinase ( MAPK ) / MAPK mitogen activated protein kinase 2 ( MAPKAPK 2 ) , we hypothesized that inhibition of p 38 MAPK activity could augment PS 341 cytotoxicity by downregulating Hsp 27 . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
Immunocytochemical localization of MAPKAPK 2 and Hsp 25 in the rat temporomandibular joint . ^^^ Furthermore , confocal microscopy with double staining was employed to demonstrate the colocalization of MAPKAPK 2 and Hsp 25 . ^^^ Double immunostaining under a confocal microscope succeeded in demonstrating the colocalization of MAPKAPK 2 and Hsp 25 immunoreactions in the cytoplasm of fibroblastic type B synoviocytes in the TMJ . ^^^ On the other hand , the macrophage like type A cells expressed MAPKAPK 2 immunoreactions but lacked Hsp 25 immunoreactivity . ^^^ The cells in the articular disk and the chondrocytes in the maturative and hypertrophic layer of the mandibular cartilage also showed intense immunoreactions for MAPKAPK 2 and Hsp 25 . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
Phosphorylation of heat shock protein 27 ( HSP 27 ) , a substrate for MAPKAPK 2 , also was detected post irradiation . ^^^ Oral administration of the p 38 inhibitor , SB 242235 , prior to UVB irradiation , blocked activation of the p 38 MAPK cascade , and abolished MAPKAPK 2 kinase activity and phosphorylation of HSP 27 . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
MAPKAPK 2 and HSP 27 are downstream effectors of p 38 MAP kinase mediated matrix metalloproteinase type 2 activation and cell invasion in human prostate cancer . ^^^ This led us to investigate mitogen activated protein kinase activated protein kinase 2 ( MAPKAPK 2 ) , a known substrate of p 38 MAP kinase , as well as heat shock protein 27 ( HSP 27 ) , a known substrate of MAPKAPK 2 , in both PC 3 and PC 3 M human prostate cells . ^^^ After transient transfection , wild type MAPKAPK 2 and HSP 27 both increased TGFbeta mediated matrix metalloproteinase type 2 ( MMP 2 ) activity , as well as cell invasion , which in turn was inhibited by SB 203580 , an inhibitor of p 38 MAP kinase . ^^^ Conversely , dominant negative MAPKAPK 2 blocked phosphorylation of HSP 27 , whereas dominant negative MAPKAPK 2 or mutant , non phosphorylateable , HSP 27 each blocked TGFbeta mediated increases in MMP 2 , as well as cell invasion . ^^^ Similarly , knock down of MAPKAPK 2 , HSP 27 or both together , by siRNA , also blocked TGFbeta mediated cell invasion . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
Butyrate mediated changes in Hsp 25 phosphorylation coincide with the activation of the p 38 MAP kinase and MAPKAPK 2 . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
Our studies also showed that extracellular alkalosis ( pH 8 . 5 ) induced MAPKAPK 2 phosphorylation ( 2 . 59 fold , 2 min ) and HSP 27 phosphorylation ( 5 . 33 fold , 2 min ) in a p 38 MAPK dependent manner , as it was inhibited with 1 micromol l ( 1 ) SB 203580 . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
The JNK pathway , Elk , MAPKAPK 2 and HSP 27 were not activated . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
MK 2 phosphorylates SRF in vitro at Ser 103 with similar efficiency as the small heat shock protein Hsp 25 and significantly better than CREB . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
Immunoprecipitation and glutathione S transferase ( GST ) pull down studies showed that Akt was associated with p 38 kinase , MK 2 , and Hsp 27 in neutrophils , and Hsp 27 dissociated from the complex upon activation . ^^^ Active recombinant MK 2 phosphorylated recombinant Akt and Akt in anti Akt , anti MK 2 , anti p 38 , and anti Hsp 27 immunoprecipitates , and this was inhibited by an MK 2 inhibitory peptide . ^^^ We conclude that Akt exists in a signaling complex containing p 38 kinase , MK 2 , and Hsp 27 and that p 38 dependent MK 2 activation functions as PDK 2 in human neutrophils . . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
Nuclear targeting of adenovirus was rescued in MK 2 ( / ) cells by overexpression of hsp 27 , an MK 2 target that enhances actin metabolism . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
Compared with heat shock protein 27 ( Hsp 27 ) , 5 LO was a weak substrate for MK 2 . ^^^ However , the addition of unsaturated fatty acids ( i . e . arachidonate 1 50 microm ) up regulated phosphorylation of 5 LO , but not of Hsp 27 , by active MK 2 in vitro , resulting in a similar phosphorylation as for Hsp 27 . 5 LO was phosphorylated also by other serine / threonine kinases recognizing the motif Arg Xaa Xaa Ser ( protein kinase A , Ca ( 2+ ) / calmodulin dependent kinase 2 ) , but these activities were not increased by fatty acids . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
Our results indicate that kinase MK 2 , which is a substrate of p 38 , becomes activated by hypoxia , leading to the phosphorylation of one of its substrates , HSP 27 . ^^^ This notion is supported by the observations that similar actin redistribution occurs in cells overexpressing constitutively active MK 2 or phosphomimicking HSP 27 mutant . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
The role of p 38 MAP kinase signaling during murine preimplantation development was investigated in the present study . p 38 MAPK , p 38 regulated or activated kinase ( PRAK ; MK 5 ) , map kinase activated protein kinase 2 ( MK 2 ) , and heat shock protein 25 ( hsp 25 ) mRNAs and proteins were detected throughout preimplantation development . ^^^ In addition , embryos treated with p 38 MAPK inhibitors displayed a complete loss of MK 2 and hsp 25 phosphorylation and also a complete loss of filamentous actin as indicated by the absence of rhodamine phalloidin staining . ^^^
Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
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Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
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Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
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Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
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Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
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Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
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Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
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Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
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Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
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Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
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Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
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Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
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Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
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Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
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Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
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Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
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Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
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Interacting proteins: P49137 and P04792	Pubmed	SVM Score :0.0
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