Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.65948217
HSP 28 , found with alpha B crystallin , in extracts of several human and bovine tissues was trapped on and coeluted with alpha B crystallin from an affinity column prepared with antibodies against alpha B crystallin . 0.65948217^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.58120042
In Rosenthal fibers ( RF ) , astrocytic inclusions that accumulate in Alexander ' s Disease , alpha B crystallin is found with hsp 27 and skeins of intermediate filaments ( IF ) of the GFAP and vimentin types . 0.58120042^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.56831686
Here we show that in a yeast two hybrid system , alphaB crystallin can specifically interact with itself as well as with alphaA crystallin and Hsp 27 . 0.56831686^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Our findings suggest that expression of pNFP and synaptophysin by ballooned neurons may reflect axonal impairment and that the presence of alpha B crystallin , srp 27 and ubiquitin may be related to the degenerative processes that neurons undergo in CJD . . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
This report deals with a comparative study on the expression of alpha B crystallin , ubiquitin , stress response protein 27 ( srp 27 ) , srp 72 and phosphorylated neurofilament protein ( pNFP ) by ballooned neurons in Pick ' s disease , Creutzfeldt Jakob disease ( CJD ) , amyotrophic lateral sclerosis ( ALS ) , leptomeningeal carcinomatosis , anterior spinal artery syndrome and pellagra . ^^^ Ubiquitin and srp 27 expression was also restricted to abnormal neurons of Pick ' s disease and CJD , but the proportion of stained cells was less than that expressing alpha B crystallin . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
The complex of hsp 27 and alpha B crystallin is also found in some other ( non transformed ) cells . ^^^ However , in most cells tested only hsp 27 and no alpha B crystallin is synthesized . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Induction of the synthesis of hsp 27 and alpha B crystallin in tissues of heat stressed rats and its suppression by ethanol or an alpha 1 adrenergic antagonist . ^^^ The levels of hsp 27 and alpha B crystallin in various tissues of rats that had been subjected to heat stress were determined by specific immunoassays . ^^^ When rats were immersed in a water bath at 42 degrees C for 20 min , the levels of hsp 27 in most tissues , including central nervous tissue , liver , lung , spleen , adrenal glands , and hypophysis , had increased dramatically 8 to 16 h after the treatment . alpha B crystallin was also induced at high levels in the liver and adrenals , but not much was induced in the central nervous tissue . ^^^ The increases in the levels of both hsp 27 and alpha B crystallin in response to heat stress were markedly inhibited when ethanol or an alpha 1 adrenergic antagonist , prazosin , was administered before , but not after , the stress period . ^^^ These results suggest that the induction of hsp 27 and alpha B crystallin , as well as of hsp 70 , in tissues of heat stressed rats is controlled by a physiological process ( es ) that is sensitive to ethanol and prazosin , and is operative for a short time during the application of heat stress . . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
HSP 27 and alpha B crystallin were coexpressed in astrocytes under unstressed and heat stressed conditions . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Whereas the small heat shock protein Hsp 27 is overexpressed in many human mammary carcinomas , we observed the frequent absence of alpha B crystallin in these tumors but not in the normal breast tissue . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Thus , HSP 27 seems to participate in the formation of certain inclusion bodies in meningioma cells , like alpha B crystallin which participates in the formation of Rosenthal fibers in astrocytes . . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Constitutive expression of human hsp 27 , Drosophila hsp 27 , or human alpha B crystallin confers resistance to TNF and oxidative stress induced cytotoxicity in stably transfected murine L 929 fibroblasts . ^^^ The genes that encode human hsp 27 , Drosophila hsp 27 , and human alpha B crystallin were placed under the control of the constitutive SV 40 early promoter and were stably introduced into L 929 cells . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Mixed polymers can be formed from any combination of alpha A crystallin , alpha B crystallin , and HSP 25 subunits . ^^^ Finally , HSP 25 is found to be a substrate for protein cross linking by tissue type transglutaminase , like alpha B crystallin . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
To test this hypothesis we studied the influence of murine Hsp 25 , human Hsp 27 , and bovine alpha B crystallin ( an eye lens protein homologous to sHsps ) on the unfolding and refolding of citrate synthase and alpha glucosidase in vitro . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Coinduction of two low molecular weight stress proteins , alpha B crystallin and HSP 28 , by heat or arsenite stress in human glioma cells . ^^^ The responses of two low molecular weight stress proteins , alpha B crystallin and HSP 28 , to various types of stress were determined quantitatively by specific immunoassays in a human glioblastoma cell line ( U 118 MG ) . ^^^ Levels of alpha B crystallin ( 2 4 ng / mg protein ) and HSP 28 ( 1 1 . 5 micrograms / mg protein ) in the soluble fraction from cells that had been cultured at 37 degrees C increased about 100 fold and 3 fold , respectively , within 24 h after heat treatment for 15 min at 45 degrees C , with a temporary decrease , due to redistribution to the insoluble fraction , during the heat treatment . ^^^ Expression of alpha B crystallin and HSP 28 mRNAs in cells was enhanced after heat stress and after exposure to arsenite . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
The C terminal domain and tail , which is the most conserved region of the alpha crystallin / small heat shock protein ( HSP ) family , was obtained from rat alpha A crystallin , bovine alpha B crystallin and mouse HSP 25 . ^^^ Whereas the C terminal part of alpha A crystallin forms dimers or tetramers , the corresponding regions of alpha B crystallin and HSP 25 form larger aggregates . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
The purpose of this study is to investigate the role of low molecular weight stress proteins , 27 kDa heat shock protein ( HSP 27 ) and alpha B crystallin , in ischemic tolerance . ^^^ The present study demonstrated that cerebral ischemia induces HSP 27 expression but not alpha B crystallin . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Coordinate and independent regulation of alpha B crystallin and hsp 27 expression in response to physiological stress . alpha Crystallins share structural and functional properties with the stress protein hsp 27 . ^^^ These polypeptides are expressed at low constitutive levels in many tissues including brain , and alpha B crystallin and hsp 27 can accumulate in central nervous system glia in a variety of neurological conditions . ^^^ We report here that heat shock and exposure to transition metals result in an increase in the steady state mRNA level of alpha B crystallin and hsp 27 in primary cultures of rat forebrain astrocytes . ^^^ Both exposure to tumour necrosis factor alpha and hypertonic conditions result in alpha B crystallin mRNA accumulation but no change in the hsp 27 mRNA level . ^^^ Under some of these conditions increased synthesis and accumulation of alpha B crystallin and hsp 27 protein are also evident . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
The primary structures of rat and human p 20 , determined with an NH 2 terminal sequenator , were highly homologous to those of alpha B crystallin and HSP27 / 28 . p 20 was present in all rat tissues examined and at high levels ( > 1 micrograms / mg protein ) in the soleus muscle , heart , and diaphragm , as are alpha B crystallin and HSP 27 . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Like HSP 27 , the presence of additional alpha B crystallin protein confers a thermoresistant phenotype to stable transfectants . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Overexpression and abnormal modification of the stress proteins alpha B crystallin and HSP 27 in Alexander disease . ^^^ In this report , we show that Alexander central nervous system ( CNS ) tissues contain elevated messenger RNA and protein levels of both alpha B crystallin and the related small heat shock protein , hsp 27 , and that Rosenthal fibers contain hsp 27 . ^^^ The alpha B crystallin and hsp 27 polypeptide isoform patterns of Alexander disease CNS are also distinct from those of control samples , suggesting that postranslational modifications may be involved in Rosenthal fiber formation . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
The localization of two small heat shock proteins , alpha B crystallin and 28 kDa heat shock protein ( HSP 28 ) , was studied immunochemically and immunohistochemically in cerebral cortex of patients with Alzheimer ' s disease ( AD ) and control groups . ^^^ Alpha B crystallin and HSP 28 are enhanced in the cerebral cortex of patients with Alzheimer ' s disease . ^^^ By specific immunoassays for alpha B crystallin and HSP 28 , we found that the concentrations of alpha B crystallin are elevated in the temporal and frontal lobes , while those of HSP 28 are elevated in the temporal , frontal , and parietal lobes in the AD brains . ^^^ Immunohistochemically , alpha B crystallin was localized in astrocytes and oligodendrocytes , while HSP 28 was present in degenerating neurons in the cerebral cortex of both AD and control brains . ^^^ However , alpha B crystallin immunoreactive astrocytes and HSP 28 immunoreactive degenerating neurons were more frequently observed in AD brains . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Comparative study on the expression of stress response protein ( srp ) 72 , srp 27 , alpha B crystallin and ubiquitin in brain tumours . ^^^ This immunohistochemical study compares the expression of stress response ( heat shock ) protein ( srp ) 72 , srp 27 , alpha B crystallin and ubiquitin in 86 primary human brain tumours and 21 carcinoma metastases to the central nervous system . ^^^ Sole expression of srp 27 , alpha B crystallin or ubiquitin was seen in several tumours . ^^^ Some meningiomas ( 3 / 23 ) and breast cancer metastases ( 4 / 10 ) co expressed srp 72 and srp 27 , and 1 / 3 of the glioblastomas co expressed srp 27 and alpha B crystallin . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Physiological and pathological changes in levels of the two small stress proteins , HSP 27 and alpha B crystallin , in rat hindlimb muscles . ^^^ The two small stress proteins , HSP 27 and alpha B crystallin , are expressed widely in normal rat tissues and abundantly in skeletal muscle . ^^^ HSP 27 was quantitated by specific immunoassay , similar to that for alpha B crystallin , with antibodies raised in rabbits against purified rat HSP 27 . ^^^ In adult rats , HSP 27 was present at high levels in tissues composed of striated muscle , and it was present at much higher levels in the soleus muscle than in the rectus femoris or extensor digitorum longus muscle , as is alpha B crystallin . ^^^ The transection of the sciatic nerve resulted in decreases in the levels of HSP 27 and alpha B crystallin in the soleus muscle of adult rats , together with increases in the levels of the two proteins in the extensor digitorum longus muscle . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Non lens expression and sequence similarities with both small ( HSP 27 ) and large ( HSP 70 ) heat shock proteins . alpha B crystallin is multifunctional , serving as both a major structural protein in the lens and a small heat shock protein ( shsp ) in other tissues in mammals . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
In addition , Western blotting of Alexander ' s disease brain homogenates revealed that the 27 kd heat shock protein ( HSP 27 ) , which is related to alpha B crystallin , accumulates along with alpha B crystallin . ^^^ Our results suggest that astrocytes in Alexander ' s disease may be involved in an as yet unknown kind of stress reaction that causes the accumulation of alpha B crystallin and HSP 27 and results in Rosenthal fiber formation . . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Expression of the murine small heat shock proteins hsp 25 and alpha B crystallin in the absence of stress . ^^^ Two related small hsp ' s , hsp 25 and alpha B crystallin exist in mice . alpha B crystallin is an abundant protein in several tissues even in the absence of stress . ^^^ In several tissues the amount of hsp 25 exceeds that accumulating in NIH 3T3 fibroblasts in response to heat stress . hsp 25 , like alpha B crystallin , exists in a highly aggregated form in the eye lens . ^^^ The expression of hsp 25 and alpha B crystallin in normal tissues suggests an essential , but distinct function of the two related proteins under standard physiological conditions . . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Responses to heat shock of alpha B crystallin and HSP 28 in U 373 MG human glioma cells . ^^^ Responses to heat shock of alpha B crystallin and small heat shock protein HSP 28 , which are expressed at considerable levels in human astroglioma U 373 MG cells ( 2 4 micrograms of each per mg soluble protein in confluent cultures ) , were analysed quantitatively by specific immunoassays . ^^^ Concentrations of alpha B crystallin and HSP 28 in soluble extracts of U 373 MG cells decreased to about 50 % of original values , with an increase in the insoluble fraction , during heat treatment for 15 min at 45 degrees C . ^^^ The concentrations of alpha B crystallin and HSP 28 increased gradually upon return to 37 degrees C , reaching and then exceeding the control levels within 5 h and 10 h , respectively , after heat shock . ^^^ During centrifugation on sucrose density gradients both alpha B crystallin and HSP 28 in extracts from untreated and heat treated cells sedimented at the same position , which corresponded to a molecular mass of > 540 kDa . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
The levels of two small stress proteins , hsp 27 and alpha B crystallin , were low in C 6 glioma cells confluency . ^^^ When cells were exposed to arsenite or heat in the presence of mastoparan , a peptide toxin from wasp venom , the induction of hsp 27 and alpha B crystallin was markedly stimulated , as detected by means of specific immunoassays , Western blot analysis , and Northern blot analysis . ^^^ Treatment of cells with 40 microM mastoparan alone barely induced the accumulation of hsp 27 and alpha B crystallin . ^^^ These results suggest that mastoparan , which is an activator of phospholipase A 2 , enhances the responses to stress of hsp 27 , alpha B crystallin and hsp 70 by increasing the metabolic activity of the arachidonic acid cascade . . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Enhancement of stress induced synthesis of hsp 27 and alpha B crystallin by modulators of the arachidonic acid cascade . ^^^ The regulation by intrinsic factors of responses to stress of two small stress proteins , hsp 27 and alpha B crystallin , was examined in C 6 rat glioma cells . ^^^ Levels of hsp 27 and alpha B crystallin were low in C 6 glioma cells in confluent cultures . ^^^ When cells were exposed to arsenite or hear in the presence of indomethacin ( 50 microM ) , an inhibitor of cyclooxygenase , or in the presence of nordihydroguaiaretic acid ( NDGA ; 50 microM ) , an inhibitor of lipoxygenase , induction of hsp 27 and alpha B crystallin was markedly stimulated as detected by specific immunoassays , Western blot analysis , and Northern blot analysis . ^^^ Induction of hsp 27 and alpha B crystallin in adrenal glands of heat stressed ( 42 degrees C for 15 min ) rats was also enhanced by prior injection of aspirin , another inhibitor of cyclooxygenase . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Recent studies have described alpha B crystallin as a member of the small heat shock protein ( HSP ) family , and the expressions of alpha crystallin related small heat shock proteins , namely HSP 27 and alpha B crystallin , in the brain appear to be regulated in a similar way by various stress conditions . ^^^ A comparative immunohistochemical analysis was performed on 198 human brain tumors to examine the expressions of HSP 27 and alpha B crystallin . ^^^ The immunohistochemical expression of HSP 27 and alpha B crystallin differed among histologic types of tumors . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
AlphaB crystallin and the small stress protein , heat shock protein of 27 kDa ( HSP 27 ) , share structural similarities and are coordinately induced by classical stress stimuli . ^^^ To examine divergence of the functional properties of these two related proteins , we have constructed stable alphaB crystallin expressing glial cell lines from the U 251 MG glioma cells , which are normally deficient in alphaB crystallin expression but constitutively express HSP 27 . ^^^ Moreover , the parental line acclimates to stepwise increases in hypertonicity and upregulates endogenous alphaB crystallin in the process but not HSP 27 . ^^^ The overexpression of HSP 27 and alphaB crystallin in NIH / 3T3 fibroblasts , a cell line that normally expresses little alphaB crystallin and no HSP 27 , does not result in increased survival . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Human hsp 27 , Drosophila hsp 27 and human alphaB crystallin expression mediated increase in glutathione is essential for the protective activity of these proteins against TNFalpha induced cell death . ^^^ Recently , we have shown that the expression of shsp from different species , such as human hsp 27 , Drosophila hsp 27 or human alphaB crystallin protected murine L 929 cells against cell death induced by tumor necrosis factor ( TNFalpha ) , hydrogen peroxide or menadione . ^^^ Our results therefore suggest that the protective activity shared by human hsp 27 , Drosophila hsp 27 and human alphaB crystallin against TNFalpha mediated cell death and probably other types of oxidative stress results from their conserved ability to raise the intracellular concentration of glutathione . . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
We also report that the expression of small stress proteins from different species , such as human hsp 27 , Drosophila Dhsp 27 , or human alphaB crystallin , confers resistance to apoptotic cell death induced by staurosporine , a protein kinase C inhibitor . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
The expression of alphaB crystallin , hsp 27 , and hsp 70 in C 6 cells increased when they were exposed to arsenite ( 50 microM for 1 h ) or heat ( 42 degrees C for 30 min ) , as detected by specific immunoassays , Western blot analysis , and Northern blot analysis . ^^^ PMA and okadaic acid also stimulated the response to heat stress of the expression of alphaB crystallin , but they barely stimulated the response to heat stress of hsp 27 . ^^^ The arsenite induced release of arachidonic acid from cells was also stimulated in the presence of PMA and / or akadaic acid , and the stimulatory effects of PMA and okadaic acid on the arsenite induced accumulation of alphaB crystallin and hsp 27 were strongly suppressed by quinacrine , an inhibitor of phospholipase A 2 . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Modulation of the stress induced synthesis of hsp 27 and alpha B crystallin by cyclic AMP in C 6 rat glioma cells . ^^^ The possible participation of cyclic AMP in the stress induced synthesis of two small stress proteins , hsp 27 and alpha B crystallin , in C 6 rat glioma cells was examined by specific immunoassays , western blot analysis , and northern blot analysis . ^^^ When C 6 cells were exposed to arsenite ( 50 100 microM for 1 h ) or heat ( 42 degrees C for 30 min ) , expression of hsp 27 and alpha B crystallin was stimulated , with levels of the two proteins reaching a maximum after 10 16 h of culture . ^^^ Northern blot analysis of the expression of mRNAs for hsp 70 , hsp 27 , and alpha B crystallin showed that the modulation of the stress induced accumulation of the three hsps by the various agents was regulated at the level of the corresponding mRNA . ^^^ These results indicate that stress responses of hsp 70 , hsp 27 , and alpha B crystallin in C 6 rat glioma cells are regulated differently and , moreover , that when the level of cyclic AMP increases in cells , the response to stress of hsp 27 is stimulated but that of alpha B crystallin is suppressed . . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
This retrospective immunohistochemical study compares the expression of five stress response ( heat shock ) proteins ( srp ' s ) [ srp 90 , srp 72 , srp 27 , alpha B crystallin and ubiquitin ] , p 53 protein and proliferating cell nuclear antigen ( PCNA ) in 118 primary brain tumors and 21 carcinoma metastases to the central nervous system . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
The central core of SEC 1 ( residues 45 126 ) is approximately 40 % identical with a corresponding segment of mammalian Hsp 27 and alphaB crystallin . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Although alpha B crystallin is thought to be one of the small HSPs , the distribution of HSP 28 in the lenses of human embryos was different from that of alpha B crystallin which we previously reported . . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Prostaglandins stimulate the stress induced synthesis of hsp 27 and alpha B crystallin . ^^^ The effects were examined of various prostaglandins ( prostaglandin A 1 , A 2 , J 2 , E 2 , and D 2 ) on the stress induced accumulation of hsp 27 and alpha B crystallin in C 6 rat glioma cells . ^^^ The levels of hsp 27 and alpha B crystallin , which were determined by specific immunoassays , were low in cells in confluent cultures . ^^^ However , there was no phosphorylation dependent mobility shift of HSF 1 and no subsequent increase in the transcription and translation for hsp 27 , alpha B crystallin , and hsp 70 . ^^^ When cells were exposed to arsenite in the presence of 10 40 microM prostaglandin , the accumulation of hsp 27 and alpha B crystallin in cells was enhanced markedly . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
No expression of ubiquitin , alpha B crystallin , srp 27 , srp 60 , or srp 72 was seen in the epithelium of the iris of the rats in Group CFA . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
The expression of two small stress proteins , alpha B crystallin and the 27 kDa heat shock protein ( HSP 27 ) , was studied quantitatively and immunohistochemically in normal kidney and renal tumors in rats . ^^^ A positive correlation was found between alpha B crystallin and HSP 27 levels limited to the renal cell tumor case ( Pearson ' s correlation coefficient , r = 0 . 68 , P < 0 . 01 ) . ^^^ Immunohistochemistry revealed the loops of Henle to be positive for alpha B crystallin , whereas HSP 27 staining was positive in glomerular and interstitial vascular walls and epithelial cells of proximal and distal tubules . ^^^ Positive immunostaining for alpha B crystallin was demonstrated in six of nine renal cell tumors ( 67 % ) studied and for HSP 27 in all of the nine cases ( 100 % ) . . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Biochemical differences between the three cell types that become detectable after transfection of the alpha A crystallin gene do exist at the level of alpha B crystallin and small heat shock protein ( HSP 27 ) expression . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
We examined the effects of reducing agents on the expression of heat shock protein 27 ( hsp 27 ) , alpha B crystallin , and hsp 70 in C 6 rat glioma cells in response to stress . ^^^ The enhanced expression of mRNAs for hsp 27 , alpha B crystallin and hsp 70 , as well as the prolonged activation of heat shock transcription factor 1 ( HSF 1 ) , were also observed in cells that had been treated with arsenite in the presence of 0 . 05 mM dithiothreitol . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Phosphorylation of alphaB crystallin , a member of the hsp 27 family , in human glioma ( U 373 MG ) cells was stimulated by exposure of the cells to various stimuli , which included heat , arsenite , phorbol 12 myristate 13 acetate ( PMA ) , okadaic acid , H2O2 , anisomycin , and high concentrations of NaCl or sorbitol , but not in response to agents that elevated intracellular levels of cyclic AMP . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Abundance and location of the small heat shock proteins HSP 25 and alphaB crystallin in rat and human heart . ^^^ BACKGROUND : In the heart , there are high constitutive levels of the two related small heat shock proteins , HSP 25 and alphaB crystallin . ^^^ METHODS AND RESULTS : Immunoblotting analysis of rat ventricular tissue at fetal , neonatal , and adult stages reveals the level of HSP 25 to decline strongly during development , whereas the level of alphaB crystallin remains nearly constant . ^^^ HSP 25 is detected in cardiomyocytes and endothelial and vascular smooth muscle cells , whereas alphaB crystallin is detected in cardiomyocytes only by immunofluorescence and immunoelectron microscopy . ^^^ In diseased and transplanted adult human hearts , HSP 25 and alphaB crystallin levels are considerably elevated compared with fetal hearts . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
However , it is unclear whether the small heat shock proteins hsp 27 and alphaB crystallin protect against ischemic injury . ^^^ METHODS AND RESULTS : Our aim was to examine whether the overexpression of hsp 27 and alphaB crystallin in neonatal and adult rat cardiomyocytes would protect against ischemic injury . ^^^ Recombinant adenovirus expressing hsp 27 or alphaB crystallin under the control of the cytomegalovirus promoter was used to infect cardiac myocytes at high efficiency as assessed by immunostaining . ^^^ CONCLUSIONS : The increased expression of hsp 27 and alphaB crystallin through an adenovirus vector system protects against ischemic injury in adult cardiomyocytes . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
The small heat shock proteins ( sHSPs ) such as alphaB crystallin and HSP 27 , which are highly expressed in muscle cells , have been suggested to play roles in maintaining myofibrillar integrity against such stresses . ^^^ This DMPK binding protein , MKBP , shows a unique nature compared with other known sHSPs : ( a ) In muscle cytosol , MKBP exists as an oligomeric complex separate from the complex formed by alphaB crystallin and HSP 27 . ( b ) The expression of MKBP is not induced by heat shock , although it shows the characteristic early response of redistribution to the insoluble fraction like other sHSPs . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Two members of the small heat shock protein family , alpha B crystallin and hsp 25 , occur at high levels in the mammalian heart . ^^^ Both proteins are stress inducible , but the level of hsp 25 is only slightly increased in cultured cardiac myocytes subjected to hyperthermic stress , while alpha B crystallin levels even remain unchanged . ^^^ Phosphorylation of alpha B crystallin and to a lesser extent also of hsp 25 is induced after the heat shock . ^^^ Directly after heat stress , alpha B crystallin and hsp 25 are partly found in detergent insoluble fractions , representing cytoskeletal / nuclear structures . ^^^ Additionally , we show by confocal laser scanning microscopy that alpha B crystallin and hsp 25 become associated with sarcomeric structures directly after the heat shock , indicating a cytoskeletal protective function . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
The effects of diethyl maleate and buthionine sulfoximine , agents that lower cellular levels of glutathione , on expression of hsp 27 and alphaB crystallin in response to stress were studied . ^^^ The latter also increased heat and CdCl 2 induced accumulation of hsp 27 and alphaB crystallin . ^^^ Northern blotting analysis revealed increase in levels of mRNAs for hsp 27 , alphaB crystallin and hsp 70 . ^^^ Although exposure of cells to diethyl maleate alone for 1 h caused neither accumulation of hsp 27 , alphaB crystallin and hsp 70 nor expression of mRNAs for these proteins , HSE binding activity of HSF was stimulated . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Pathological changes in levels of three small stress proteins , alphaB crystallin , HSP 27 and p 20 , in the hindlimb muscles of dy mouse . ^^^ Using three different analyses , we investigated the levels of the three small stress proteins alphaB crystallin , HSP 27 and p 20 in the slow twitch soleus muscle and fast twitch tibialis anterior muscle of normal and dy mice . ^^^ In contrast , the levels of alphaB crystallin , HSP 27 and p 20 of dy mouse were reduced in slow twitch type muscle ( soleus muscle ) . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Heat shock , but not the reactive state per se , induces increased expression of the small stress proteins hsp 25 and alpha B crystallin in glial cells in vitro . ^^^ The stress proteins hsp 25 and alpha B crystallin are found in increased concentrations in reactive astrocytes of brains undergoing neurodegeneration . ^^^ In order to characterize this reaction , we investigated the expression of hsp 25 and alpha B crystallin during growth and after stress ( heat shock ) in glial cells in vitro . ^^^ In primary rat brain cultures , hsp 25 was present in actively dividing astrocytes that were positive for glial fibrillary acidic protein . alpha B crystallin was found predominantly in oligodendrocytes . ^^^ Heat shock resulted in increased concentrations of hsp 25 and alpha B crystallin in astrocytes , without any detectable changes in intracellular localization , as detectable with confocal laser microscopy . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Phosphorylation of alphaB crystallin and HSP 27 is induced by similar stressors in HeLa cells . ^^^ Three members of the small heat shock protein family , alphaA , alphaB crystallin , and HSP 27 , confer thermoresistance upon their overexpression in mammalian cells . ^^^ We here report the influence of heat shock and other kinds of stress on the phosphorylation of alphaA , alphaB crystallin , and HSP 27 in stably transfected HeLa cells . ^^^ It is observed that alphaB crystallin becomes phosphorylated upon exposure to the same inducers as is HSP 27 , although to a lesser extent . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
In contrast , the small heat shock proteins Hsp 25 and alpha B crystallin are not expressed in these cells and can not be induced upon heat shock . ^^^ Silencing of the hsp 25 and alpha B crystallin genes in P 388 cells by DNA methylation could also be excluded since 5 azacytidine treatment does not influence expression of these genes . ^^^ Taken together , the data suggest cell line specific differences in a mechanism of regulation of hsp 25 transcription , which interferes with the activation of the promoter by HSF 1 and which may also affect the alpha B crystallin gene . . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Astrocytes in the Tg73 . 2 cultures appear odd shaped and enlarged , express increased levels of GFAP ( both human and mouse ) , and express alphaB crystallin protein , Hsp 27 protein , and vimentin protein . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Curcumin , a major component of turmeric , a seasoning commonly used in Indian food , and a known antioxidant , anti inflammatory and anti carcinogenic agent , is a potent stimulator of the stress induced expression of Hsp 27 , alphaB crystallin and Hsp 70 . ^^^ Induction of Hsp 27 , alphaB crystallin and Hsp 70 in the liver and adrenal glands of heat stressed ( 42 degrees C for 20 min ) rats was also enhanced by prior injection of curcumin ( 20 mg / kg body weight ) . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Co expression of heat shock transcription factor 1 ( HSF 1 ) and the catalytically active fragment of PKN induced the accumulation of alphaB crystallin but not HSP 27 and HSP 70 in HeLa S 3 cells . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Microtubular integrity after ischemia was significantly better preserved in cells overexpressing constitutive Hsp 70 ( 21 . 7 + / 1 . 6 ) or alphaB crystallin ( 18 . 0 + / 2 . 7 ) but not in cells overexpressing inducible Hsp 70 ( 11 . 5 + / 0 . 8 ) or Hsp 27 ( 14 . 0 + / 2 . 2 ) . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Different concentrations of two small stress proteins , alphaB crystallin and HSP 27 in human urological tumor tissues . ^^^ Concentrations of two small stress proteins , alphaB crystallin and the 27 kDa heat shock protein ( HSP 27 ) were quantitated in tissues of the human normal genitourinary system and their tumors . ^^^ Levels of HSP 27 in renal cell carcinomas ( mean + / SE : 1450+ / 262 ng / mg protein , n = 15 ) were significantly higher than in normal kidney ( the cortex : 540+ / 99 ng / mg protein , n = 13 ; the medulla : 600+ / 106 ng / mg protein , n = 13 ) while those of alphaB crystallin tended to be increased without statistical significance . ^^^ Both alphaB crystallin and HSP 27 could be immunohistochemically localized in the normal kidney and renal cell carcinoma tissues . . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
In the present study , we examined the effect of vasopressin on the induction of the low molecular weight heat shock proteins heat shock protein 27 ( HSP 27 ) and alphaB crystallin in an aortic smooth muscle cell line , A 10 cells . ^^^ Vasopressin induced a time dependent accumulation of HSP 27 and alphaB crystallin . ^^^ The EC 50 values for vasopressin were 2 ( HSP 27 ) and 4 nmol / L ( alphaB crystallin ) . ^^^ Vasopressin induced increases in the levels of the mRNAs for HSP 27 and alphaB crystallin . 12 O Tetradecanoylphorbol 13 acetate ( TPA ) , a protein kinase C ( PKC ) activating phorbol ester , induced an accumulation of HSP 27 ( EC 50 , 20 nmol / L ) and alphaB crystallin ( EC 50 , 2 nmol / L ) . ^^^ Staurosporine and calphostin C , inhibitors of PKC , significantly reduced the vasopressin induced accumulation of HSP 27 and alphaB crystallin as well as that induced by TPA . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Identification of a site of Hsp 27 binding with Hsp 27 and alpha B crystallin as indicated by the yeast two hybrid system . ^^^ The small heat shock proteins ( sHsp ) , including Hsp 27 and alphaB crystallin , usually form large oligomers in cells . ^^^ We used the yeast two hybrid system to investigate binding of full length rat Hsp 27 or fragments of Hsp 27 to full length rat Hsp 27 or alphaB crystallin molecules . ^^^ These cDNAs were each transfected into yeast that had been transfected to express full length rat Hsp 27 or alphaB crystallin fused with the DNA binding domain of Gal4p . ^^^ Results indicated that the N terminal domain of Hsp 27 consisting of amino acids 1 124 did not bind to Hsp 27 or alphaB crystallin . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
We especially explored protective functions of inducible heat shock protein 70 , the mitochondrial heat shock protein 60 and 10 , and the small heat shock proteins HSP 27 and alphaB crystallin against ischemic , reoxygenation mediated injury using transgenic animals and hearts under in vivo conditions and in isolated cardiac myocyte derived cells using adenoviral vectors . ^^^ Inducible hsp 70 , hsp 60 and hsp 10 when coexpressed , and hsp 27 and alphaB crystallin have an overall protective effect against ischemic injury as determined by the release of enzymes like creatine kinase and LDH . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
These human GFAP inclusions did not contain the endogenous vimentin or beta tubulin , and the intermediate filament and microtubular networks of the transfected cells appeared normal . alphaB crystallin and hsp 25 were associated with the GFAP inclusions . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Levels of Hsp 27 , and alphaB crystallin were elevated in these cells . ^^^ The mechanism of this resistance appears to involve the increased expression of Hsp 27 and alphaB crystallin . . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Intermediate filament interactions can be altered by HSP 27 and alphaB crystallin . ^^^ HSP 27 and alphaB crystallin are both members of the small heat shock protein family . alphaB crystalllin has been proposed to modulate intermediate filaments and recently a mutation in alphaB crystallin has been identified as the genetic basis of desmin related myopathy . ^^^ Here we report that HSP 27 like alphaB crystallin is associated with glial fibrillary acidic protein and vimentin intermediate filament networks in unstressed U373MG astrocytoma cells . ^^^ These data show that the presence of HSP 27 or alphaB crystallin could not prevent filament collapse and suggest that the purpose of this association is more than just filament binding . ^^^ In contrast , inclusion of alphaB crystallin or HSP 27 prevented gel formation but not filament assembly . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Responses of heat shock proteins hsp 27 , alphaB crystallin , and hsp 70 in rat brain after kainic acid induced seizure activity . ^^^ We determined the changes in the levels of the mammalian small heat shock protein of 25 28 kDa ( hsp 27 ) and the hsp alphaB crystallin in various regions of rat brain after kainic acid induced seizure activity by means of specific immunoassays . ^^^ The levels of protein sulfhydryl group and glutathione were significantly reduced in the hippocampus of rats at 24 h after the seizure , which might have enhanced the expressions of hsp 27 and alphaB crystallin . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
The small heat shock proteins alpha B crystallin and HSP 27 exert a protective effect in response to simulated ischemia . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Dementia , gliosis and expression of the small heat shock proteins hsp 27 and alpha B crystallin in Parkinson ' s disease . ^^^ In AD , there is a massive reactive gliosis and increased expression of the small heat shock proteins ( hsp ) hsp 27 and alpha B crystallin . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
METHODS : In alphaB crystallin , residues H 101 to R 120 are highly conserved between alphaB crystallin and alphaA crystallin ( 85 % identity ) , and between alphaB crystallin and the small heat shock protein hsp 27 ( 80 % identity ) . ^^^ CONCLUSIONS : Despite the high conservation in this area of sequence between alphaB crystallin , alphaA crystallin , and the small heat shock protein hsp 27 , mutations F118A , K103L / H104I , and E110H / H111E did not significantly alter chaperone like activity . . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
In AD brains , the small heat shock proteins ( sHsps ) alphaB crystallin and Hsp 27 occur at increased levels and colocalize with these plaques . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
MATERIAL AND METHODS : We have tested the serum of patients with clinically definite MS ( n=30 ) , other inflammatory neurological disease ( n=22 ) , non inflammatory neurological disease ( n=42 ) and healthy individuals ( n=23 ) for systemic humoral responses to bovine alphaB crystallin , to the homologous chaperone protein , alphaA crystallin , and to another small Hsp , Hsp 27 . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Here we report the cloning and expression of alphaB crystallin from the zebrafish . 5 ' and 3 ' RACE was used to isolate a 900 bp transcript that contained insertions and deletions that differentiate it from both alphaA crystallin and HSP 27 . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Selective stimulation of Hsp 27 and alphaB crystallin but not Hsp 70 expression by p 38 MAP kinase activation . ^^^ The levels of Hsp 27 and alphaB crystallin in C 6 rat glioma cells , that had been heated at 43 degrees C for 30 min with a subsequent culture for 16 h at 37 degrees C , were markedly increased . ^^^ The exposure of the cells to a low concentration ( 0 . 1 3 microg / ml ) of anisomycin for a few hours after heat stress stimulated the accumulation of the small stress proteins Hsp 27 and alphaB crystallin , but not that of Hsp 70 . ^^^ The levels of mRNAs for Hsp 27 and alphaB crystallin but not that for Hsp 70 increased in cells that had been exposed to heat and subsequently for 2 h to 0 . 1 3 microg / ml anisomycin . ^^^ The heat induced accumulations of Hsp 27 and alphaB crystallin were also stimulated by cycloheximide , another stimulator of p 38 MAP kinase . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
In the present study , we have demonstrated that transient focal ischemia in rats dramatically induced HSP 27 but not alpha B crystallin ( alphaBC ) , both of which are members of sHSPs , in reactive astrocytes . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
We found that Hsp 27 and alphaB crystallin readily exchanged with fluorescence labeled alphaA crystallin , but not with other proteins structurally unrelated to sHsps . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
In this paper , we develop the former work by performing extensive characterization of five of the six sHSPs so far identified , that is , HSP 27 , alphaB crystallin , p 20 , MKBP / HSPB2 , and HSPB 3 , omitting lens specific alphaA crystallin . ^^^ Moreover , these sHSPs form an oligomeric complex with an apparent molecular mass of 150 kDa that is completely independent of the oligomers formed by HSP 27 , alphaB crystallin , and p 20 . ^^^ The functional divergence among sHSPs in different oligomers is also demonstrated in several ways : 1 ) an interaction with myotonic dystrophy protein kinase , which has been suggested to be important for the maintenance of myofibril integrity , was observed only for MKBP / HSPB2 ; 2 ) a myotube specific association with actin bundles was observed for HSP 27 and alphaB crystallin , but not for MKBP / HSPB2 ; and 3 ) sHSPs whose mRNAs are induced by heat shock are alphaB crystallin and HSP 27 . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Sham operated kidneys had similarly small amounts of hsp 25 and alphaB crystallin in cortex and glomeruli , with substantially greater amounts of alphaB crystallin versus hsp 25 in outer and inner medulla . ^^^ Ischemia resulted in significantly increased hsp 25 ( and hsp70i ) but variable alphaB crystallin levels in cortex and outer medulla , and progressively decreased glomerular hsp 25 phosphorylation . ^^^ In sham operated kidneys , hsp 25 localized to glomeruli , vessels , and collecting ducts , with alphaB crystallin primarily in medullary thin limbs and collecting ducts . ^^^ After ischemia , hsp 25 accumulated in proximal tubules in cortex and outer medulla , while alphaB crystallin labeling became nonhomogeneous in outer medulla , and increased in Bowman ' s capsule . ^^^ It is concluded that : ( 1 ) There is striking differential expression of hsp 25 and alphaB crystallin in various renal compartments ; and ( 2 ) Renal ischemia results in differential accumulation of hsp 25 and alphaB crystallin , with hsp 25 part of a generalized stress response in renal proximal tubular cells , which may play a role in recovery from ischemia induced actin filament disruption . . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
No expression of ubiquitin , alphaB crystallin , srp 27 , srp 60 , or srp 72 was seen in the epithelium of the iris in group CFA rats . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
The stress kit : a new method based on competitive reverse transcriptase polymerase chain reaction to quantify the expression of human alphaB crystallin , Hsp 27 , and Hsp 60 . ^^^ We describe a reverse transcriptase polymerase chain reaction method for the semiquantitative detection of mRNAs encoding the human heat shock proteins alphaB crystallin , Hsp 27 , and Hsp 60 . ^^^ Furthermore , we illustrate the utility of this technique by quantifying increased expression of alphaB crystallin , Hsp 27 , and Hsp 60 mRNA in astroglioma cells on heat shock . . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Here , we present parallel cryo electron microscopy studies of five different sHSP assemblies : Methanococcus jannaschii HSP16 . 5 , human alphaB crystallin , human HSP 27 , bovine native alpha crystallin , and the complex of alphaB crystallin and unfolded alpha lactalbumin . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
In this study cytokine induced expression of hsp 60 , hsp 27 and alphaB crystallin was studied in cultures of primary human adult astrocytes at the mRNA as well as at the protein level . ^^^ Upregulation of hsp 27 , however , was primarily induced by immunoregulatory cytokines like IL 4 , IL 6 and TGF beta whereas alphaB crystallin expression was found to be enhanced by the pro inflammatory cytokine TNF alpha only . ^^^ These results show that in human astrocytes induced expression of hsp 27 and alphaB crystallin is dependent on the presence of a defined set of stimuli , while induced expression of hsp 60 is a much less selective event . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
We have investigated whether translocation of constitutive low molecular weight stress proteins ( alphaB crystallin and HSP 27 ) to the myofilament / cytoskeletal compartment occurs during ischemic preconditioning and assessed if this is causally associated with cardioprotection . ^^^ Triton insoluble preparations from fresh or aerobically perfused rat hearts ( n=4 / group ) contained relatively little alphaB crystallin ( 96 + / 43 and 43 + / 36 units respectively ) or HSP 27 ( 177 + / 32 and 101 + / 26 units respectively ) . ^^^ Two hours of aerobic perfusion following the preconditioning protocol resulted the return of alphaB crystallin and HSP 27 to near control levels ( 189 + / 14 units and 252 + / 24 units , respectively ) . ^^^ Thus , three cycles of 5 min hypercarbia + 5 min normocarbia increased alphaB crystallin to 628 + / 30 units ( P < 0 . 05 ) and HSP 27 to 1353 + / 53 units . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Some reports indicate that small heat shock proteins , Hsp 27 and alphaB crystallin , colocalize in the plaques , but their functions are not known . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
The expression pattern of alphaB crystallin was similar to that of HSP 25 , another small heat shock protein , but differed from the classic heat shock protein HSP 70 . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Compared with srp 60 negative tumours , srp 60 positive tumours coexpressed one or more stress related proteins , among which srp 90 , srp 72 , srp 27 , alphaB crystallin and ubiquitin occurred with higher frequencies ; a high correlation between srp 60 and the other five srps ( 0 . 88 0 . 97 , p < 0 . 01 , Pearson correlation coefficient ) was observed in srp 60 positive tumours . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
We also demonstrated that the delayed induction of alpha B crystallin and HSP 27 immunoreactivities in the hippocampus of epileptic animals was repressed to the levels seen in control animals with preadministration of the selective nNOS inhibitor 7 nitroindazole ( 7 NI ) . ^^^ Together , these data suggest a role for alpha B crystallin and HSP 27 in reactive gliosis and / or in delayed neuronal death proceeded after KA induced seizure . . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Almost all of the rEGCs were immunopositive for glial fibrillary acidic protein ( GFAP ) and heat shock protein 27 ( HSP 27 ) , and some of them showed immunopositivity with alphaB crystallin . ^^^ We considered that the rEGCs originated from overexpression and accumulation of alphaB crystallin and HSP 27 in GFAP positive oligodendroglial cells due to various pathological conditions . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
After heat shock , HSP 25 , alpha B crystallin and HSP 70 were up regulated , while after oxidative stress the specific induction of HSP 32 and alpha B crystallin was observed . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
The small heat shock proteins ( sHSP ) , alpha B crystallin and Hsp 27 are chaperone molecules that maintain the integrity of intermediate filament ( IF ) network and prevent unfolding of cellular proteins induced by stress . ^^^ In this study , we determined the effect of elevated hydrostatic pressure ( HP ) in the synthesis , distribution and co localization of alpha B crystallin and Hsp 27 with IF in cultured ONH astrocytes . ^^^ Cells were analyzed by immunocytochemistry , Western blot and immunoprecipitation using antibodies to Hsp 27 , alpha B crystallin , vimentin or GFAP . ^^^ GFAP and vimentin co localized with Hsp 27 and alpha B crystallin in the perinuclear area . ^^^ Western blot and metabolic labeling detected increased synthesis of Hsp 27 , GFAP and vimentin but no change in alpha B crystallin . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
A distinctive histopathological feature of several neurodegenerative diseases , including corticobasal degeneration , argyrophilic grain disease , progressive supranuclear palsy , and Pick ' s disease , are achromatic nerve cells that express small heat shock proteins , such as alphaB crystallin or hsp 27 , and develop in specific telencephalic cortical areas and subcortical nuclei . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Hsp 27 , a member of the family of the small heat shock proteins , showed a similar interaction property with alphaB crystallin . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Here , we investigated the regulation of expression and localization of other HSPs , including HSP 70 , HSP 25 , and alphaB crystallin , in the kidney of rats undergoing long term administration of Ang 2 ( 0 . 7 mg . kg ( 1 ) . d ( 1 ) ) . ^^^ Immunoblot analysis demonstrated that Ang 2 increased renal expression of HSP 70 and HSP 25 , as well as HO 1 , but that expression of alphaB crystallin was unaffected by this treatment . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Serum antigen titer to hsps ( hsp 27 , alpha B crystallin , human & bacterial hsp 60 ) was higher in both POAG and NTG patients than in normal subjects . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
We here report that upon proteasome inhibition , alpha B crystallin and Hsp 25 translocate from the detergent soluble cytosolic fraction to the detergent insoluble nuclear / cytoskeletal fraction . ^^^ Although phosphorylation of both alpha B crystallin and Hsp 25 is induced , this does not seem to be essential for the translocation . ^^^ Immunocytochemistry revealed that alpha B crystallin and Hsp 25 , which show a diffuse cytoplasmic staining in unstressed H9c2 cells , colocalize with F actin upon proteasomal inhibition . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Alpha B crystallin , ubiquitin and heat shock protein 27 ( hsp 27 ) belong to a class of proteins that are overexpressed in response to pathological conditions associated with increased cellular stress . ^^^ In the present study , brain sections of old rhesus monkeys ( Macaca mulatta ; n=10 ; mean age , 29 . 4 years ) and baboons ( Papio anubis ; n=8 ; mean age , 18 . 3 years ) were examined for ubiquitin , alpha B crystallin and hsp 27 immunopositive structures . ^^^ To further clarify the origin of the pallido nigral spheroids , single and double immunostaining was performed for hsp 27 , alpha B crystallin and the astroglial marker glial fibrillary acidic protein ( GFAP ) as well as for neuronal markers against neurofilament and dendritic microtubule associated protein 2 . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
In the mouse myocardial microvascular cell line , MyEnd , alphaB crystallin as well as the heat shock proteins HSP 70i and HSP 25 display a low constitutive expression but can be significantly upregulated by sodium arsenite stress . ^^^ Osmotic stress also resulted in strong upregulation of alphaB crystallin and HSP 70i but not of HSP 25 . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
H2O2 alone induced the phosphorylation of HSP 27 Ser 86 and Ser 45 / Ser 59 of its homologue alphaB crystallin . ^^^ Despite effective kinase inhibition , H2O2 still disaggregated HSP 27 , but not alphaB crystallin . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Inhibition of proteasomes induces accumulation , phosphorylation , and recruitment of HSP 27 and alphaB crystallin to aggresomes . ^^^ HSP 27 and alphaB crystallin accumulated in both soluble and , more prominently , insoluble fractions after exposure to MG 132 , a proteasome inhibitor . ^^^ Enhanced expression of mRNAs for HSP 27 and alphaB crystallin was observed , suggesting transcriptional activation . ^^^ Phosphorylation of HSP 27 and alphaB crystallin in cells treated with MG 132 was enhanced concomitantly with activation of p 38 and p44 / 42 MAP kinase pathways . ^^^ Immunofluorescence analysis revealed that exposure to proteasome inhibitors induced the formation of aggresomes in U 373 MG cells , to which HSP 27 and alphaB crystallin were recruited . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
We demonstrate that dexamethasone enhances alpha B crystallin and HSP 27 expression in normal and desmin related myopathy derived muscle cells . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
We also demonstrate that ectopic expression of alpha B crystallin , but not the closely related small heat shock protein Hsp 27 , renders C2C12 myoblasts resistant to differentiation induced apoptosis . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
AlphaB crystallin ( alphaBC ) and heat shock protein 27 ( hsp 27 ) are members of the family of small heat shock proteins ( shsps ) , which exert a role as molecular chaperones by binding unfolded or denatured proteins , thereby suppressing irreversible protein aggregation and consecutive cell damage . ^^^ In the present study we analyzed the expression and localization of alphaB crystallin and hsp 27 in various congenital myopathies by means of indirect immunofluorescence , immunogold electron microscopy and Western blotting . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
We assessed whether eccentric exercise modulates the expression of proteinases ( calpains 1 , 2 and 3 , proteasome , cathepsin B+L ) , muscle structural proteins ( alpha sarcoglycan and desmin ) , and the expression of the heat shock proteins Hsp 27 and alphaB crystallin . ^^^ The alpha sarcoglycan protein level was decreased immediately after exercise and at day 1 , whereas the desmin level peaked at day 14 . alphaB crystallin and Hsp 27 protein levels were increased at days 1 and 14 . ^^^ Our results suggest that the differential expression of calpain 2 and 3 mRNA levels may be important in the process of exercise induced muscle damage , whereas expression of alpha sarcoglycan , desmin , alphaB crystallin and Hsp 27 may be essentially involved in the subsequent remodelling of myofibrillar structure . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Innervation dependent phosphorylation and accumulation of alphaB crystallin and Hsp 27 as insoluble complexes in disused muscle . ^^^ Levels and phosphorylation states of the two small molecular chaperones , alphaB crystallin and Hsp 27 , in disused rat soleus muscles were determined by Western blot analysis of extracts with antibodies recognizing each of the two proteins and their phosphorylated serine residues . ^^^ High phosphorylation of alphaB crystallin at Ser 59 ( and to a certain extent , at Ser 45 ) and of Hsp 27 at Ser 15 and Ser 85 , along with phosphorylated , active states of p 38 and p44 / 42 mitogen activated protein kinases were maintained during hind limb suspension but promptly returned to control levels within a 5 day recovery period . ^^^ However , the responses of alphaB crystallin and Hsp 27 in suspended soleus muscles did not appear with ipsilateral transection of the sciatic nerve trunk , indicating mediation by nerve activity . ^^^ The fact that ubiquitinated proteins accumulated in the insoluble fractions of suspended soleus muscle further suggests participation of alphaB crystallin and Hsp 27 in quality control of proteins in disused soleus muscle , with involvement of nerve activity dependent processes . . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Here we report that , in mice , HSF 1 deficiency reduces cardiac expression of Hsp 25 , alphaB crystallin and Hsp 70 , but not Hsp 60 and Hsp 90 . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Both HP and HS improved recovery of left ventricular function with translocation of PKC delta from the cytosol to the nuclear fraction and induction of heat shock proteins , HSP 27 , HSP 70 , and alphaB crystallin . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
The level of expression of three small HSPs , alphaB crystallin , HSP 20 , HSP 27 , as well as the phosphorylation levels of HSP 27 and p 38 , were higher in cultured , asynchronously growing SMCs originating from left interior mammary artery ( LIMA ) than those originating from aorta , saphenous vein , and umbilical vein , validating the choice of SMCs from LIMA as a model system in our study . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
We studied the expression of 3 HSPs ( alphaB crystallin , HSP 27 , and HSP 70 ) in transgenic mice overexpressing human mutant ( G93A and G37R ) SOD 1 , using a combination of immunohistochemistry and immunoblotting . ^^^ Quantitative Western blot analysis demonstrated alphaB crystallin and HSP 27 to be upregulated in the spinal cord of mutant SOD 1 mice compared to mice overexpressing wild type SOD 1 . ^^^ In contrast to HSP 27 , alphaB crystallin was localized exclusively in the cytoplasm of reactive glial cells . . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
BACKGROUND : The small heat shock proteins HSP 20 , HSP 25 , alphaB crystallin , and myotonic dystrophy kinase binding protein ( MKBP ) may regulate dynamic changes in the cytoskeleton . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
The levels of lens metallothioneins ( Ig , If , Ih , Ie , and IIa ) and alpha crystallin / small heat shock ( alphaA crystallin , alphaB crystallin , and HSP 27 ) genes were analyzed by semiquantitative and quantitative competitive RT PCR . ^^^ RESULTS : Lens metallothioneins ( Ig , If , Ih , Ie , and IIa ) and alpha crystallin / small heat shock genes ( alphaA crystallin , alphaB crystallin , and HSP 27 ) were differentially induced by specific metals in SRA01 / 04 human lens epithelial cells . ^^^ Cd ( 2+ ) and Zn ( 2+ ) , but not Cu ( 2+ ) , induced the metallothioneins , whereas Cd ( 2+ ) and Cu ( 2+ ) , but not Zn ( 2+ ) , induced alphaB crystallin and HSP 27 . alphaA crystallin was induced by Cu ( 2+ ) only . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
We reported the physiological roles of HSP 20 , HSP 27 , and alpha B crystallin in platelet function in vitro and ex vivo . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
We have been reported that Hsp 27 and alpha B crystallin were recruited to aggresome when cells were treated with proteasome inhibitors . ^^^ We recently examined the interaction of R120G alpha B crystallin and Hsp 27 in mammalian cells and found that expression of R120G alpha B crystallin caused formation of inclusion bodies and co expression of Hsp 27 inhibited this formation of inclusion bodies . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Enhanced stability of alpha B crystallin in the presence of small heat shock protein Hsp 27 . ^^^ Lens alpha crystallin , alpha A and alpha B crystallin , and Hsp 27 are members of the small heat shock protein family . ^^^ Both alpha A and alpha B crystallin are expressed in the lens and serve as structural proteins and as chaperones , but alpha B crystallin is also expressed in nonlenticular organs where Hsp 27 , rather than alpha A crystallin , is expressed along with alpha B crystallin . ^^^ It is not known what additional function Hsp 27 has besides as a heat shock protein , but it may serve , as alpha A crystallin does in the lens , to stabilize alpha B crystallin . ^^^ In this study , we investigate aspects on conformation and thermal stability for the mixture of Hsp 27 and alpha B crystallin . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Surprisingly , TGF beta 1 treatment for 48 h markedly decreased the LEDGF , Hsp 27 , and alphaB crystallin promoter activities with the decrease of abundance of LEDGF mRNA and protein . ^^^ The TGF beta 1 induced down regulation of LEDGF , Hsp 27 , and alphaB crystallin promoters activity was reversed by cotransfection with a plasmid expressing LEDGF . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Expression of the two closely related heat shock proteins ( HSPs ) , alpha B crystallin and Hsp 27 , was studied in various tissues of the developing pig . ^^^ Expression of alpha B crystallin was maximum in the lens while expression of Hsp 27 was absent . ^^^ Increases in alpha B crystallin expression were observed in brain , liver and kidney while Hsp 27 was increased in kidney at later stages . ^^^ In the different parts of the gastrointestinal tract , increased expression of alpha B crystallin was seen following birth and weaning while increased expression of Hsp 27 was restricted to weaning . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
We treated rats with cyclosporine A alone , or combined with melatonin , and with melatonin alone ( as controls ) for 40 days and analysed the renal abundance and distribution of two sHSPs , HSP 25 and alpha B crystallin . ^^^ In conclusion , HSP 25 and alpha B crystallin are overexpressed in the rat kidney treated with cyclosporine A but are similar to controls after combined melatonin . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Nine proteins have been assigned to date to the superfamily of mammalian small heat shock proteins ( sHsps ) : Hsp 27 ( HspB 1 , Hsp 25 ) , myotonic dystrophy protein kinase binding protein ( MKBP ) ( HspB 2 ) , HspB 3 , alphaA crystallin ( HspB 4 ) , alphaB crystallin ( HspB 5 ) , Hsp 20 ( p 20 , HspB 6 ) , cardiovascular heat shock protein ( cvHsp [ HspB 7 ] ) , Hsp 22 ( HspB 8 ) , and HspB 9 . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
In this study , the small heat shock protein ( sHSP ) chaperones , alpha B crystallin and HSP 27 , are identified as nuclear speckle components in unstressed cells in tissue culture . ^^^ Both alpha B crystallin and HSP 27 were immunolocalised to nuclear speckles ( interchromatin granule clusters ) . ^^^ While alpha B crystallin was preferentially localised to speckles as shown by colocalisation with non snRNP , SC 35 , as well as the snRNP components Sm and U1A , HSP 27 was also seen associated with the nucleolar compartment , indicating a subtle difference between these closely related sHSPs . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Hsp 27 suppresses the formation of inclusion bodies induced by expression of R120G alpha B crystallin , a cause of desmin related myopathy . ^^^ In addition , we examined interactions of this mutant alpha B crystallin with Hsp 27 , another representative sHSP . ^^^ In immunofluorescence studies , we found 15 25 % of R120G alpha B crystallin expressing cells to contain multiple cytosolic inclusion bodies , in which Hsp 27 was also localized . ^^^ When R120G alpha B crystallin and Hsp 27 were transiently co expressed in HeLa cells , the amount of R120G alpha B crystallin in the soluble fraction was greater than with expression of R120G alpha B crystallin alone . ^^^ Moreover , co expression resulted in reduced formation of inclusion bodies , suggesting that Hsp 27 acts as a molecular chaperone for R120G alpha B crystallin . . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Moreover , chaperones Hsp 25 and alphaB crystallin specifically co fractionated with insoluble SOD 1 . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
When overexpressing alphaB crystallin or Hsp 27 , cap dependent initiation of translation was protected but no effect was seen on cap independent initiation of translation . ^^^ Phosphorylation of alphaB crystallin and Hsp 27 is known to significantly decrease their chaperone activity ; therefore , we tested phosphorylation mutants of these proteins in this system . ^^^ AlphaB crystallin needs to be in its non phosphorylated state to give protection , whereas phosphorylated Hsp 27 is more potent in protection than the unphosphorylatable form . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Ten consecutively collected surgical specimens meeting the histological criteria for AVM were immunohistochemically stained using primary antibodies for nestin , HSP 27 and alphaB crystallin . ^^^ Nestin , HSP 27 and alphaB crystallin mRNA expressions were examined by reverse transcriptase polymerase chain reaction ( RT PCR ) . ^^^ These cells also expressed sHSP ( HSP 27 , alphaB crystallin ) that maintain the integrity of the IF network and prevent unfolding of cellular proteins induced by various stresses . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Expression of alphaB crystallin was induced efficiently only by proteasome inhibition and not by heat shock while expression of HSP 27 was induced efficiently by both proteasome inhibition and heat shock . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Lengthening contractions also resulted in immediate translocation of constitutively expressed HSP 25 and alphaB crystallin from the soluble to the insoluble fraction of muscle homogenates , and cryosections showed translocation from a diffuse , cytosolic localization to striations that corresponded to z disks . ^^^ Lengthening contraction induced translocation of HSP 25 and alphaB crystallin was associated with phosphorylation of these small HSPs , which may trigger their protective activity . ^^^ In summary , these findings demonstrate loss of z disk and membrane scaffolding proteins immediately after lengthening contractions , and concomitant translocation of HSP 25 and alphaB crystallin to the z disk , which may help to stabilize or repair cytoskeletal elements at this site . . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
We studied expression of these proteins in porcine lens , brain , heart , liver , kidney , lung , skeletal muscle , stomach , and colon , and found a ubiquitous expression of Hsp 20 and HspB 8 as earlier reported for Hsp 27 and alphaB crystallin . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
The proteins up regulated were mostly HSPs ( heat shock proteins ) and antioxidative stress proteins , including HSP 70 , aldose reductase , haem oxygenase 1 , HSP 27 , ferritin light chain and alphaB crystallin . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Prolonged incubation with MG 132 resulted in the increased expression of heat shock protein ( hsp ) 25 , alphaB crystallin and hsp 70 , with a population of hsp 25 associating with the eIF4F complex in a p 38 mitogen activated protein kinase dependent manner . ^^^ Although MG 132 had little effect on the colocalization of eIF4E and eIF4GI , it promoted the SB 203580 sensitive association of eIF4GI and hsp 25 , an effect not observed with alphaB crystallin . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Heat stress induced localization of small heat shock proteins in mouse myoblasts : intranuclear lamin A / C speckles as target for alphaB crystallin and Hsp 25 . ^^^ We examined the effect of heat stress on localization of two sHsps , alphaB crystallin and Hsp 25 , and of Hsc 70 , a member of a different class of heat shock proteins ( Hsps ) , in both undifferentiated and differentiated mouse C2C12 cells . ^^^ Under normal conditions , alphaB crystallin and Hsp 25 are found in the cytoplasm ; only alphaB crystallin is also found in the nucleus , distributed in a speckled pattern . ^^^ Dual staining experiments using C2C12 myoblasts show that alphaB crystallin and Hsp 25 , but not Hsc 70 , colocalize with the intranuclear lamin A / C and the splicing factor SC 35 , suggesting interactions of sHsps and intranuclear lamin A / C . ^^^ This differential entry of alphaB crystallin and Hsp 25 into the nuclei of myoblasts and myotubes upon heat stress may have functional role in the development and / or in the maintenance of muscle cells . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Hsp 27 , Hsp 20 and HspB 8 are both lysine and glutamine donors , alphaB crystallin only is a lysine donor , HspB 2 a glutamine donor , and HspB 3 no substrate at all . ^^^ We also observed that alphaB crystallin , Hsp 27 and Hsp 20 associate with Abeta in vitro , and can be readily crosslinked by tTG . . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Comparison of the small heat shock proteins alphaB crystallin , MKBP , HSP 25 , HSP 20 , and cvHSP in heart and skeletal muscle . ^^^ In the present study five sHSPs ( alphaB crystallin , MKBP , HSP 25 , HSP 20 , and cvHSP ) were investigated with respect to similarities and differences of their expression in heart and skeletal muscle under normal and ischemic conditions . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
After the end of the treatment , the thoracic aortae were removed and treated for morphological ( Sirius Red ) and immunohistochemical evaluation ( HSP 25 , alphaB crystallin and HSP 47 ) . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Single TM treatments induced only modest increases in the amounts of hsp 25 , alphaB crystallin , and inducible hsp 70 . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
In addition to the cytoskeletal elements , several heat shock proteins ( HSP 27 , HSP 60 , HSP 70 , grp 75 , alphaB crystallin ) , resident endoplasmic reticulum ( ER ) proteins ( calreticulin , grp78 / BiP , ERp 29 ) , proteins associated with neurodegenerative diseases ( ubiquitin C terminal hydrolase L 1 , rat ortholog of human DJ 1 / Park7 , gamma synuclein , superoxide dismutase 1 ) , anti oxidant proteins ( peroxiredoxins 1 and 6 ) , and metabolic proteins ( e . g . , phosphoglycerate kinase 1 ( PGK 1 ) , alpha enolase , aldolase C / Zebrin 2 ) were included among the axonally synthesized proteins . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Small heat shock proteins HSP 27 and alphaB crystallin : cytoprotective and oncogenic functions . ^^^ Heat shock protein 27 ( HSP 27 ) and alphaB crystallin are ubiquitous small heat shock proteins whose expression is induced in response to a wide variety of physiological and environmental insults . ^^^ Both HSP 27 and alphaB crystallin were also proposed to participate in the development of neurodegenerative diseases and malignant tumors in which their overexpression could induce drug resistance . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
To investigate whether Hsps might exert a similar protective effect under physiological conditions in the kidney , we first evaluated the HSF 1 dependent expression of several Hsps , including Hsp 25 , alphaB crystallin ( alphaBC ) , Hsp 70 , and Hsp 90 . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
The change in expression of three proteins [ ciliary neurotrophic factor ( CNTF ) , alphaB crystallin , and heat shock protein 27 ( hsp 27 ) ] was confirmed by quantitative Western blotting . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
A CAT assay revealed transcriptional repression of lens epithelium derived growth factor ( LEDGF ) , HSP 27 , and alphaB crystallin promoter activities in these cells . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
The relative levels of alphaB crystallin , HspB 8 , Hsp 20 , Hsp 27 , Hsp 60 , and Hsp 70 as well as nitric oxide synthases ( NOS ) ( endothelial NOS , inducible NOS , neuronal NOS ) were examined by Western blot analysis . ^^^ This observation dissociates the response to low oxygenation of alphaB crystallin and other stress associated proteins including Hsp 27 , and it indicates that heme oxygenase is not alone among HSPs in its oxygen related gene expression . . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Alexander disease is a fatal neurological illness characterized by white matter degeneration and the formation of astrocytic cytoplasmic inclusions called Rosenthal fibers , which contain the intermediate filament glial fibrillary acidic protein ( GFAP ) , the small heat shock proteins HSP 27 and alphaB crystallin , and ubiquitin . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
In this study , we estimated the endoplasmic reticulum ( ER ) stress induced response of Hsp 27 and alphaB crystallin in mammalian cells . ^^^ Treatment targeting the ER with tunicamycin or thapsigargin induced the phosphorylation of Hsp 27 but not of alphaB crystallin in U 373 MG cells , increase being observed after 2 10 h and decline at 24 h . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
HS induced rapid decreases of soleus muscle mass , most Hsps ( alphaB crystallin , Hsp 90 , Hsp 70 , Hsp 27 , and p 20 ) and tubulin contents in soleus muscle , while heat shock cognate 70 kDa protein ( Hsc 70 ) did not decrease . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
RESULTS : At the mRNA level , the proteasome inhibitor , MG 132 , caused a > 10 fold increase in HSP 27 and a small increase ( 1 . 2 to 1 . 6 fold ) in alphaB crystallin but no change in HSP 70 or 90 . ^^^ At the protein level , a more than twofold increase in HSP 27 and 90 , a marked increase in HSP 70 , but no significant change in alphaB crystallin , was observed . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Proteins included those involved in metabolism , i . e . aconitase and pyruvate dehydrogenase , and those related to stress responses and cytoskeletal reorganization , i . e . cofilin , Hsp 27 , and alpha b crystallin . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
As well as being intensely immunoreactive for ubiquitin , alphaB crystallin , Hsp 27 , and Hsp 70 , spheroids were immunoreactive for Hsp 32 ( heme oxygenase 1 ) , Hsp 40 , Hsp 60 , and Hsp 90 . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
We analyzed the expression of hsp 27 and alphaB crystallin in skeletal muscle specimens of patients with desminopathies , plectinopathies , myotilinopathy , and other myofibrillar myopathies by means of differential centrifugation , 2D gel electrophoresis , Western blotting , and mass spectrometry . ^^^ Hsp 27 P82 and P 15 as well as alphaB crystallin P 59 and P 45 are the major serine phosphorylation isoforms in normal and diseased human skeletal muscle . 2D gel electrophoresis revealed spots of hsp 27 in a range of pH 5 . 3 6 . 4 in samples of all skeletal muscle specimens , except for the seven desminopathies . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
After western blotting , alphaB crystallin , HSP 27 , and HSP 70 expressions were determined in newborn controls and piglets exposed to 1 or 4 h hypoxia ( 5 % O 2 , 95 % N 2 ) allowed to recover from 1 to 68 h . ^^^ Surprisingly , alphaB crystallin was overexpressed in the stomach and colon in animals submitted to hypoxia , whereas HSP 27 and HSP 70 expression remained stable . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Two families showed changes in 2DGE migration or abundance with H2O2 treatment : the peroxiredoxins and two small heat shock protein ( Hsp ) family members : heat shock 27 kDa protein 1 ( Hsp 25 ) and alphaB crystallin . ^^^ Hsp 25 also shifted to lower pI values but this was attributable to phosphorylation . alphaB crystallin migration was unchanged but its abundance decreased . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Mammalian small heat shock proteins including Hsp 27 and alpha B crystallin are constitutively expressed in various adult and embryonic tissues including skeletal and cardiac muscle . ^^^ Here , we assessed the temporospatial dynamics of zebrafish Hsp 27 ( hsp 27 ) and alpha B crystallin ( cryab ) gene expression using reverse transcriptase PCR ( RT PCR ) and hsp 27 expression by in situ hybridization . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Hsp 27 , but not alphaB crystallin , also hastened rephosphorylation of SRp 38 dephosphorylated a potent inhibitor of splicing after a heat shock , although it did not prevent dephosphorylation by a heat shock . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Phosphoprotein staining and Western blotting demonstrated that the phosphorylation of alpha B crystallin at serine ( Ser ) 59 site and of HSP 27 at both Ser 78 and Ser 82 sites increased in CHF . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
The effect of alphaB crystallin and Hsp 27 on the availability of translation initiation factors in heat shocked cells . ^^^ The mechanism of the translational thermotolerance provided by the small heat shock proteins ( sHsps ) alphaB crystallin or Hsp 27 is unknown . ^^^ We show here that Hsp 27 , but not alphaB crystallin , increased the pool of mobile stress granule associated enhanced green fluorescent protein ( EGFP ) eukaryotic translation initiation factor ( eIF ) 4E in heat shocked cells , as determined by fluorescence recovery after photobleaching . ^^^ Expression of the C terminal fragment of GADD 34 , which causes constitutive dephosphorylation of eIF2alpha , fully compensated for the stimulatory effect of alphaB crystallin on protein synthesis in heat shocked cells , but only partially for that of Hsp 27 . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
We reported on a physiological role of HSP 20 , HSP 27 and alphaB crystallin on platelet function in vitro and ex vivo . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
In contrast , exercise did not induce a significant increase in either HSP 27 , alphaB crystallin , SOD 2 ( MnSOD ) protein content , or the activity of SOD and catalase . ^^^ When examining baseline protein levels , HSC 70 , HSP 27 , and alphaB crystallin appeared consistently expressed between subjects , whereas HSP 70 and MnSOD displayed marked individual variation of up to 3 and 1 . 5 fold , respectively . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Muscles leading to darker meat had a more oxidative metabolism , indicated by more abundant mitochondrial enzymes of the respiratory chain , hemoglobin , and chaperone or regulator proteins ( HSP 27 , alphaB crystallin , and glucose regulated protein 58 kDa ) . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
The aim of this study was to investigate the association of the chaperones Hsp 20 , HspB 2 , alphaB crystallin and Hsp 27 with the pathological lesions of AD brains . ^^^ Both Hsp 27 and alphaB crystallin were also observed in astrocytes associated with both SPs and CAA . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Hsp 20 , Hsp 27 and alphaB crystallin , but not HspB2 / B3 , bound to Abeta ( both D Abeta 1 40 and Abeta 1 42 ) and reduced or completely inhibited aggregation of D Abeta 1 40 into mature fibrils but did not affect Abeta 1 42 aggregation . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Heat shock protein 27 ( Hsp 27 ) and alpha B crystallin ( alphaBC ) are small heat shock proteins that stabilize the myofilament during stress . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
In vitro , monocyte derived DCs were stimulated with recombinant alphaA crystallin , alphaB crystallin , HSP 20 , HSPB 8 , and HSP 27 . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
The majority of central chromatolysis like enlarged neurons , which are frequently seen in the early stages of olivary hypertrophy on ipsilateral lesions , showed a marked expression of alpha B crystallin ; however , HSP 27 did not show increased expression in those neurons . ^^^ In the later stages of olivary hypertrophy , increased expressions of alpha B crystallin varied in the remaining neurons and the expression of HSP 27 increased in hypertrophied astrocytes , although the expression of alpha B crystallin in hypertrophic astrocytes was not prominent . ^^^ The accumulation of alpha B crystallin and HSP 27 may represent responses to pathologic conditions . . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
As beta crystallins form heteromers , a process that involves transient exposure of hydrophobic interfaces , we have examined whether in vivobeta crystallin assembly is enhanced by protein chaperones , either small heat shock proteins , Hsp 27 or alphaB crystallin , or Hsp 70 . ^^^ We show here that betaA 4 crystallin is abundantly expressed in HeLa cells , but rapidly degraded , irrespective of the presence of Hsp 27 , alphaB crystallin or Hsp 70 . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
The Alexander disease causing glial fibrillary acidic protein mutant , R416W , accumulates into Rosenthal fibers by a pathway that involves filament aggregation and the association of alpha B crystallin and HSP 27 . ^^^ These aggregates , termed `` Rosenthal fibers , ' ' contain the protein chaperones alpha B crystallin and HSP 27 as well as glial fibrillary acidic protein ( GFAP ) , an intermediate filament ( IF ) protein found almost exclusively in astrocytes . ^^^ The aggregates have several important features in common with Rosenthal fibers , including the association of alpha B crystallin and HSP 27 . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Using the yeast two hybrid method , quantitative fluorescence resonance energy transfer in live cells , and cross linking , we found aberrantly increased interactions of mutant HSP 22 forms with themselves , with wild type HSP 22 , and with the other sHSPs , alphaB crystallin , and HSP 27 . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
Human endometrium expresses a molecular repertoire which includes the heat shock proteins ( Hsps ) Hsp 27 , Hsp 60 , Hsp 70 , Hsp 90 , and alpha crystallin B chain . ^^^
Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
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Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
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Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
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Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
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Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
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Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
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Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
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Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
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Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
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Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
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Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
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Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
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Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
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Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
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Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
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Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
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Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
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Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
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Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
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Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
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Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
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Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
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Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
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Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
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Interacting proteins: P02511 and P04792	Pubmed	SVM Score :0.0
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