| Interacting proteins: O14558 and P04792 |
Pubmed |
SVM Score :0.0 |
| Nine proteins have been assigned to date to the superfamily of mammalian small heat shock proteins ( sHsps ) : Hsp 27 ( HspB 1 , Hsp 25 ) , myotonic dystrophy protein kinase binding protein ( MKBP ) ( HspB 2 ) , HspB 3 , alphaA crystallin ( HspB 4 ) , alphaB crystallin ( HspB 5 ) , Hsp 20 ( p 20 , HspB 6 ) , cardiovascular heat shock protein ( cvHsp [ HspB 7 ] ) , Hsp 22 ( HspB 8 ) , and HspB 9 . ^^^ |
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| Interacting proteins: O14558 and P04792 |
Pubmed |
SVM Score :0.0 |
| Precontraction of bovine carotid artery smooth muscle with serotonin followed by relaxation with forskolin was associated with increases in the phosphorylation of HSP 27 and HSP 20 . ^^^ |
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| Interacting proteins: O14558 and P04792 |
Pubmed |
SVM Score :0.0 |
| Cyclic nucleotide dependent relaxation of CSA induced contractions was associated with increases in the phosphorylation of another small heat shock protein , HSP 20 , and decreases in the phosphorylation of the MLC 20 , and some isoforms of HSP 27 . ^^^ |
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| Interacting proteins: O14558 and P04792 |
Pubmed |
SVM Score :0.0 |
| Recent evidence suggests that a group of small HSPs may modulate vascular smooth muscle contraction ( HSP 27 ) and / or relaxation ( HSP 20 ) . ^^^ This impaired vasorelaxation was associated with increases in the phosphorylation of HSP 27 and decreases in forskolin induced phosphorylation of HSP 20 . ^^^ |
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| Interacting proteins: O14558 and P04792 |
Pubmed |
SVM Score :0.0 |
| Two small heat shock proteins , HSP 27 and HSP 20 , have been implicated in the regulation of the contraction and relaxation of smooth muscle . ^^^ |
|
| Interacting proteins: O14558 and P04792 |
Pubmed |
SVM Score :0.0 |
| This was associated with an increase in the aortic expression and phosphorylation of HSP 27 , which was localized to the vascular smooth muscle , but a decrease in the amount of phosphorylated ( P ) HSP 20 . ^^^ To determine if P HSP 27 inhibits phosphorylation of HSP 20 , P HSP 27 was added to a reaction mixture containing recombinant HSP 20 and the catalytic subunit of cAMP dependent protein kinase . ^^^ P HSP 27 inhibited phosphorylation of HSP 20 in a concentration dependent manner . ^^^ These data demonstrate that P HSP 27 can inhibit phosphorylation of HSP 20 . ^^^ The increase in P HSP 27 and decrease in P HSP 20 were associated with reduced cyclic nucleotide dependent vascular smooth muscle relaxation in response to behavioral stress in vivo , an effect similar to that observed previously in response to cellular stress in vitro . . ^^^ |
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| Interacting proteins: O14558 and P04792 |
Pubmed |
SVM Score :0.0 |
| Although other , more distantly related , members of the Hsp 20 family , i . e . the plastidic pea Hsp 21 , the Drosophila Hsp 23 and the mouse Hsp 25 , were well expressed in tobacco protoplasts and formed homo oligomers of 200 700 kDa , none of them could be recruited to HSG complexes . . ^^^ |
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| Interacting proteins: O14558 and P04792 |
Pubmed |
SVM Score :0.0 |
| HSP 20 is associated with elements of the contractile machinery such as another small heat shock protein ( HSP 27 ) , actin , and myosin . ^^^ |
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| Interacting proteins: O14558 and P04792 |
Pubmed |
SVM Score :0.0 |
| Recent data have implicated two small heat shock proteins ( HSPs ) , namely HSP 20 and HSP 27 , in the regulation of vascular tone . ^^^ Increases in the phosphorylation of HSP 20 are associated with vasorelaxation , and increases in the phosphorylation of HSP 27 are associated with impaired vasorelaxation . ^^^ Physiological responses were correlated with the expression and phosphorylation of HSP 20 and HSP 27 by using one and two dimensional immunoblots . ^^^ These changes were associated with decreased expression of both total and phosphorylated HSP 20 and increases in the amount of phosphorylated HSP 27 . ^^^ CONCLUSION : In this model , impaired relaxation of cerebral vessels after SAH was associated with increases in the amount of phosphorylated HSP 27 and decreases in the expression and phosphorylation of HSP 20 . ^^^ |
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| Interacting proteins: O14558 and P04792 |
Pubmed |
SVM Score :0.0 |
| The level of expression of three small HSPs , alphaB crystallin , HSP 20 , HSP 27 , as well as the phosphorylation levels of HSP 27 and p 38 , were higher in cultured , asynchronously growing SMCs originating from left interior mammary artery ( LIMA ) than those originating from aorta , saphenous vein , and umbilical vein , validating the choice of SMCs from LIMA as a model system in our study . ^^^ |
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| Interacting proteins: O14558 and P04792 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : The small heat shock proteins HSP 20 , HSP 25 , alphaB crystallin , and myotonic dystrophy kinase binding protein ( MKBP ) may regulate dynamic changes in the cytoskeleton . ^^^ |
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| Interacting proteins: O14558 and P04792 |
Pubmed |
SVM Score :0.0 |
| We reported the physiological roles of HSP 20 , HSP 27 , and alpha B crystallin in platelet function in vitro and ex vivo . ^^^ |
|
| Interacting proteins: O14558 and P04792 |
Pubmed |
SVM Score :0.0 |
| HSP 20 co immunoprecipitated with both actin and alpha actinin , but not with HSP 27 or paxillin . ^^^ |
|
| Interacting proteins: O14558 and P04792 |
Pubmed |
SVM Score :0.0 |
| We studied expression of these proteins in porcine lens , brain , heart , liver , kidney , lung , skeletal muscle , stomach , and colon , and found a ubiquitous expression of Hsp 20 and HspB 8 as earlier reported for Hsp 27 and alphaB crystallin . ^^^ |
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| Interacting proteins: O14558 and P04792 |
Pubmed |
SVM Score :0.0 |
| The mixing of wild type human Hsp 27 and Hsp 20 resulted in a slow , temperature dependent formation of hetero oligomeric complexes , with apparent molecular mass values of 100 and 300 kDa , which contained approximately equal amounts of Hsp 27 and Hsp 20 subunits . ^^^ A 3D mutant of Hsp 27 mixed with Hsp 20 rapidly formed a hetero oligomeric complex with an apparent molecular mass of 100 kDa , containing approximately equal quantities of two small heat shock proteins . . ^^^ |
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| Interacting proteins: O14558 and P04792 |
Pubmed |
SVM Score :0.0 |
| It is known that different HSPs are expressed in skeletal muscle , namely , small HSPs ( including ubiquitin , alpha B crystallin , HSP 20 and HSP 27 ) , HSP 70 , HSP 60 and HSP 90 . ^^^ |
|
| Interacting proteins: O14558 and P04792 |
Pubmed |
SVM Score :0.0 |
| Hsp 27 , Hsp 20 and HspB 8 are both lysine and glutamine donors , alphaB crystallin only is a lysine donor , HspB 2 a glutamine donor , and HspB 3 no substrate at all . ^^^ We also observed that alphaB crystallin , Hsp 27 and Hsp 20 associate with Abeta in vitro , and can be readily crosslinked by tTG . . ^^^ |
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| Interacting proteins: O14558 and P04792 |
Pubmed |
SVM Score :0.0 |
| Comparison of the small heat shock proteins alphaB crystallin , MKBP , HSP 25 , HSP 20 , and cvHSP in heart and skeletal muscle . ^^^ In the present study five sHSPs ( alphaB crystallin , MKBP , HSP 25 , HSP 20 , and cvHSP ) were investigated with respect to similarities and differences of their expression in heart and skeletal muscle under normal and ischemic conditions . ^^^ |
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| Interacting proteins: O14558 and P04792 |
Pubmed |
SVM Score :0.0 |
| Recent data have implicated two small heat shock proteins ( HSP ) , namely HSP 20 and HSP 27 , in the regulation of vascular tone . ^^^ Phosphorylation of HSP 20 is associated with vasorelaxation , whereas phosphorylation of HSP 27 is associated with vasoconstriction . ^^^ Protein levels of HSP 20 and HSP 27 were detected by Western blot analysis . ^^^ |
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| Interacting proteins: O14558 and P04792 |
Pubmed |
SVM Score :0.0 |
| Accordingly , we observed differential regulation of molecular chaperones ( hsp 20 , hsp 27 , reticuloplasmin ER 60 ) and of proteins implicated in reactive aldehyde detoxification ( aldehyde dehydrogenase , glutathione transferase , glyoxalase ) . ^^^ |
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| Interacting proteins: O14558 and P04792 |
Pubmed |
SVM Score :0.0 |
| The relative levels of alphaB crystallin , HspB 8 , Hsp 20 , Hsp 27 , Hsp 60 , and Hsp 70 as well as nitric oxide synthases ( NOS ) ( endothelial NOS , inducible NOS , neuronal NOS ) were examined by Western blot analysis . ^^^ |
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| Interacting proteins: O14558 and P04792 |
Pubmed |
SVM Score :0.0 |
| The small heat shock proteins , HSP 20 and HSP 27 , coordinately regulate vascular smooth muscle tone . ^^^ Phosphorylated HSP 20 is associated with vasorelaxation , and phosphorylated HSP 27 inhibits the phosphorylation of HSP 20 and relaxation . ^^^ Segments were frozen and homogenized , and extracted proteins were separated by one and two dimensional gel electrophoresis , transferred to Immobilon ( Millipore ) , and probed with anti cGMP dependent protein kinase ( anti PKG ) , HSP 20 , HSP 27 , and phosphoHSP 27 antibodies . ^^^ |
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| Interacting proteins: O14558 and P04792 |
Pubmed |
SVM Score :0.0 |
| Inactivity induced modulation of Hsp 20 and Hsp 25 content in rat hindlimb muscles . ^^^ After 7 days of SI , Hsp 20 and Hsp 25 levels in the soleus , plantaris , and adductor longus muscles were lower than in control rats , whereas Hsp 20 was unchanged and Hsp 25 increased in the tibialis anterior . ^^^ |
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| Interacting proteins: O14558 and P04792 |
Pubmed |
SVM Score :0.0 |
| Using peptide mass fingerprint and tandem mass spectrometry analysis these proteins were identified as myoglobin , two slow twitch isoforms of myosin light chain and two small heat shock proteins ( HSP 20 and HSP 27 ) . ^^^ The protein levels of myoglobin , myosin light chain and HSP 20 were higher in red muscle , whereas HSP 27 was higher in white muscle . ^^^ |
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| Interacting proteins: O14558 and P04792 |
Pubmed |
SVM Score :0.0 |
| We show that pseudophosphorylation ( mimicry of phosphorylation ) of the homologous phosphorylation sites Ser 15 and Ser 16 in Hsp 27 and Hsp 20 , respectively , modulates characteristics of these sHsps that can be detected by their ability to activate reporter genes in suitable 2 hybrid assays . ^^^ We conclude that pseudophosphorylation of Hsp 27 and Hsp 20 at their N termini results in conformational changes that can be detected by their interaction with other sHsps . ^^^ |
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| Interacting proteins: O14558 and P04792 |
Pubmed |
SVM Score :0.0 |
| The aim of this study was to investigate the association of the chaperones Hsp 20 , HspB 2 , alphaB crystallin and Hsp 27 with the pathological lesions of AD brains . ^^^ We observed extracellular expression of Hsp 20 , Hsp 27 and HspB 2 in classic SPs , and Hsp 20 expression in diffuse SPs . ^^^ |
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| Interacting proteins: O14558 and P04792 |
Pubmed |
SVM Score :0.0 |
| Hsp 20 , Hsp 27 and alphaB crystallin , but not HspB2 / B3 , bound to Abeta ( both D Abeta 1 40 and Abeta 1 42 ) and reduced or completely inhibited aggregation of D Abeta 1 40 into mature fibrils but did not affect Abeta 1 42 aggregation . ^^^ |
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| Interacting proteins: O14558 and P04792 |
Pubmed |
SVM Score :0.0 |
| In vitro , monocyte derived DCs were stimulated with recombinant alphaA crystallin , alphaB crystallin , HSP 20 , HSPB 8 , and HSP 27 . ^^^ |
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| Interacting proteins: O14558 and P04792 |
Pubmed |
SVM Score :0.0 |
| In this work , we examined the mechanism of Hsp 20 interaction with Abeta 1 40 and compared its activity to that of other small heat shock proteins , carrot Hsp17 . 7 and human Hsp 27 . ^^^ |
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| Interacting proteins: O14558 and P04792 |
Pubmed |
SVM Score :0.0 |
| By database ( www . toxodb . org ) search , five parasite sHsps ( Hsp 20 , Hsp 21 , Hsp 28 , Hsp 29 , and the previously characterized Hsp30 / Bag1 ) were identified . ^^^ Hsp 20 is located at the apical end of the cell , Hsp 28 is located inside the mitochondrion , Hsp 29 showed a membrane associated labeling , and Hsp 21 appeared throughout the cytosol of the parasites . ^^^ |
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| Interacting proteins: O14558 and P04792 |
Pubmed |
SVM Score :0.0 |
| Five proteins , namely cofilin , lactoylglutathione lyase , substrate protein of mitochondrial ATP dependent proteinase SP 22 , HSP 27 and HSP 20 , were changed in both LD and ST muscles during post mortem storage . ^^^ |
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| Interacting proteins: O14558 and P04792 |
Pubmed |
SVM Score :0.0 |
| We reported on a physiological role of HSP 20 , HSP 27 and alphaB crystallin on platelet function in vitro and ex vivo . ^^^ |
|
| Interacting proteins: O14558 and P04792 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14558 and P04792 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14558 and P04792 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14558 and P04792 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14558 and P04792 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14558 and P04792 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14558 and P04792 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14558 and P04792 |
Pubmed |
SVM Score :0.0 |
| NA |
|