| Interacting proteins: P04629 and P62993 |
Pubmed |
SVM Score :1.0604721 |
| Complex formation between Trk and SHC , SHC tyrosine phosphorylation , and association of SHC with Grb 2 were mediated by autophosphorylation at Y 490 in Trk [ sequence : see text ] . 1.0604721^^^ |
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| Interacting proteins: P04629 and P62993 |
Pubmed |
SVM Score :0.0 |
| Consistent with this model , FRS 2 binds Grb 2 , Crk , the SH 2 domain containing tyrosine phosphatase SH PTP 2 , the cyclin dependent kinase substrate p 13 ( suc 1 ) , and the Src homology 3 ( SH 3 ) domain of Src , providing a functional link between TrkA , cell cycle , and multiple NGF signaling effectors . ^^^ |
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| Interacting proteins: P04629 and P62993 |
Pubmed |
SVM Score :0.0 |
| Direct binding of the signaling adapter protein Grb 2 to the activation loop tyrosines on the nerve growth factor receptor tyrosine kinase , TrkA . ^^^ We demonstrate that the signaling adapter , Grb 2 , binds directly to the neurotrophin receptor tyrosine kinase , TrkA . ^^^ Grb 2 binding to TrkA is independent of Shc , FRS 2 , phospholipase Cgamma 1 , rAPS , and SH2B and is observed in in vitro binding assays , yeast two hybrid assays , and in co immunoprecipitation assays . ^^^ Grb 2 binding to TrkA is mediated by the central SH 2 domain , requires a kinase active TrkA , and is phosphotyrosine dependent . ^^^ By analyzing a series of rat TrkA mutants , we demonstrate that Grb 2 binds to the carboxyl terminal residue , Tyr ( 794 ) , as well as to the activation loop tyrosines , Tyr ( 683 ) and Tyr ( 684 ) . ^^^ |
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| Interacting proteins: P04629 and P62993 |
Pubmed |
SVM Score :0.0 |
| In addition , we showed that active Src phosphorylates TrkA directly in vitro , and that Src associates with TrkA through Grb 2 after NGF stimulation . ^^^ These results suggest that constitutively active Src that associates with TrkA through Grb 2 after NGF stimulation participates in TrkA phosphorylation and in turn enhances the mitogen activated protein kinase signaling in SK N MC cells . . ^^^ |
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| Interacting proteins: P04629 and P62993 |
Pubmed |
SVM Score :0.0 |
| Consistent with the suppression , NAC inhibited NGF induced activation of TrkA , formation of receptor complexes comprising TrkA , Shc , Grb 2 , and Sos , and activation of phospholipase Cgamma and phosphatidylinositol 3 kinase . ^^^ |
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| Interacting proteins: P04629 and P62993 |
Pubmed |
SVM Score :0.0 |
| However , Grb 2 does not bind to NGF activated Trk receptors . ^^^ Mitogenic signaling of NGF in NIH 3T3 cells ectopically expressing Trk receptors also takes place without detectable association between Grb 2 and Trk . ^^^ |
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| Interacting proteins: P04629 and P62993 |
Pubmed |
SVM Score :0.0 |
| While Shc is also found bound to the activated NGF receptor , Trk , no complexes were detectable that contained both Trk and Grb 2 or Sos . ^^^ Higher concentrations of TRK Y490P inhibit tyrosine phosphorylation of Shc and the formation of Shc complexes with Grb 2 : this results in strong inhibition of Ras activation by NGF and partial inhibition of Ras activation by EGF . ^^^ |
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| Interacting proteins: P04629 and P62993 |
Pubmed |
SVM Score :0.0 |
| The oncogenic versions of the Ret and Trk tyrosine kinases bind Shc and Grb 2 adaptor proteins . ^^^ In addition , in cells containing either Ret or Trk oncoproteins , Shc proteins are constitutively phosphorylated on tyrosine and bound to Grb 2 . ^^^ Only in in vitro experiments were Ret and Trk oncoproteins shown to bind the SH 2 region of Grb 2 . ^^^ Finally , when proto Trk product is stimulated by NGF , Shc phosphorylation and association with Grb 2 are induced . ^^^ |
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| Interacting proteins: P04629 and P62993 |
Pubmed |
SVM Score :0.0 |
| We show that phosphorylation of tyrosine 490 , but not 785 , of Trk is essential for activation of both Ras and PI 3 kinase in vivo , correlating with tyrosine phosphorylation of Shc and binding of Shc to the adaptor Grb 2 and the Ras exchange factor Sos . ^^^ |
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| Interacting proteins: P04629 and P62993 |
Pubmed |
SVM Score :0.0 |
| We analyzed the elements of the pathway that lead to this activation , and we observed that calmodulin antagonists completely block the initial Raf 1 activation without affecting the function of upstream elements , such as Ras , Grb 2 , Shc , and Trk . ^^^ |
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| Interacting proteins: P04629 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04629 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04629 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04629 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04629 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04629 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04629 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04629 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04629 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04629 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04629 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04629 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04629 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04629 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04629 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
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