| Interacting proteins: P04629 and P46108 |
Pubmed |
SVM Score :0.0 |
| Nerve growth factor induces a multimeric TrkA receptor complex in neuronal cells that includes Crk , SHC and PLC gamma 1 but excludes P130CAS . ^^^ Using receptor activated immunoprecipitation followed by analysis of the immune complexes by SDS PAGE and immunoblotting , we show that NGF induces the association of TrkA with c Crk 2 in a multimeric complex that also includes SHC and PLC gamma 1 . ^^^ While the tyrosine phosphorylation of TrkA , SHC and PLC gamma 1 increased with time in the presence of NGF and was inhibited by the tyrosine kinase inhibitor K252a , the state of tyrosine phosphorylation of c Crk 2 did not appear to change with NGF treatment . ^^^ Immunodepletion studies demonstrated that the interaction of c Crk 2 with TrkA not only occurs indirectly through the SHC proteins , but may also involve another mode of binding . ^^^ These results clearly implicate c Crk 2 in the NGF signaling pathway and support the concept that more than one signaling molecule known to participate in the activation of Ras associates with TrkA upon NGF treatment . . ^^^ |
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| Interacting proteins: P04629 and P46108 |
Pubmed |
SVM Score :0.0 |
| Consistent with this model , FRS 2 binds Grb 2 , Crk , the SH 2 domain containing tyrosine phosphatase SH PTP 2 , the cyclin dependent kinase substrate p 13 ( suc 1 ) , and the Src homology 3 ( SH 3 ) domain of Src , providing a functional link between TrkA , cell cycle , and multiple NGF signaling effectors . ^^^ |
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