| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
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| NA |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| A new cis element is involved in the HER 2 gene overexpression in human breast cancer cells . ^^^ |
|
| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| In a consecutive and unselected series of 178 cases of carcinoma in situ of the breast ( CIS ) , comprising both ductal ( DCIS ) and lobular type ( LCIS ) , and a series of 48 cases of invasive carcinoma ( IC ) with predominance of DCIS , the association between histopathology , immunohistochemical markers ( ER , PgR , MIB 1 , c erbB 2 , and p 53 ) , and DNA ploidy was investigated , in order to discriminate biologically different groups . ^^^ Small nuclear CIS , whether LCIS or DCIS , on the contrary , were DNA diploid with low proliferation , and no cases showed overexpression of c erbB 2 and p 53 . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| Degradation signals in ErbB 2 dictate proteasomal processing and immunogenicity and resist protection by cis glycine alanine repeat . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| Our previous studies have identified a new cis element in the ERBB 2 promoter which is involved in the gene ' s overexpression . ^^^ This cis element , located 501 bp upstream from the main ERBB 2 transcription initiation site , binds a transcription factor called HTF ( HER 2 transcription factor ) . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| Immunohistochemical c erbB 2 proto oncogene expression and nuclear DNA distribution patterns were assessed in 119 formalin fixed paraffin embedded surgical specimens of human mammary carcinomas in situ ( CIS ) . ^^^ The results of the current study indicate that immunohistochemical expression of the c erbB 2 proto oncogene product is closely related to the histopathologic subtype and the nuclear DNA content of mammary CIS . ^^^ Examples of CIS that are c erbB 2 immunoreactive and DNA aneuploid seem to have a significantly higher risk for the subsequent development of infiltrating mammary carcinoma . . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| FISH analysis showed erbB 2 gene amplification and p 53 deletions in selected CIS , as well as a marked chromosome 17 copy number heterogeneity in all six CIS examined . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| The authors studied 30 consecutive archival cases of pure breast carcinoma in situ ( CIS ) for estrogen receptor ( ER ) , progesterone receptor ( PR ) , and c erbB 2 oncogene product . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| Relative risks ( RRs ) and confidence intervals ( CIs ) for recurrence in relation to HER 2 status were estimated using a multivariate Cox proportional hazards model . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| Small nuclear CIS , whether LCIS or DCIS , on the contrary , were DNA diploid with low proliferation , and no cases showed overexpression of c erbB 2 and p 53 . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| RI HER 2 was significantly more frequent in tumors with two or more recurrences ( 40 . 7 % versus 15 . 8 % , P = 0 . 010 ) and in those with carcinoma in situ ( CIS ) ( 35 . 4 % versus 15 . 9 % , P = 0 . 029 ) . ^^^ In addition , a relative increase in HER 2 was associated with the number of recurrences and the presence of CIS . . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| An overexpression of Her2 / neu is found in 10 20 % of invasive urothelial carcinomas and occasionally in Cis ( 5 % ) . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| We evaluated a new modification of in situ hybridization , the chromogenic in situ hybridization ( CISH ) , which enables detection of HER 2 / neu gene copies with conventional peroxidase reaction . ^^^ We conclude that CISH , using conventional bright field microscopy in evaluation , is a useful alternative for determination of HER 2 / neu amplification in paraffin embedded tumor samples , especially for confirming the immunohistochemical staining results . . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| Chromogenic in situ hybridization ( CISH ) detection of the HER 2 / neu oncogene is a newly developed in situ hybridization method that utilizes a robust and unique sequence DNA probe labeled with digoxygenin , and sequential incubations with antidigoxygenin fluorescein , antifluorescein peroxidase , and diaminobenzidine . ^^^ In this study , we examined 20 archival specimens of human breast carcinoma using CISH , and we correlated findings with immunohistochemical findings for HER 2 / neu . ^^^ Seven out of eight carcinoma cases found to overexpress immunoreactive HER 2 / neu also demonstrated HER 2 / neu gene amplification following CISH analysis . ^^^ We found that CISH was a specific , sensitive and easily applicable method for the detection of HER 2 / neu gene amplification , which may be used together with immunohistochemical examination for the evaluation of patients with breast carcinoma . . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| Of 126 prostate tumors , chromogenic in situ hybridization ( CISH ) revealed only 1 case containing borderline ( six to eight copies ) amplifications of ERBB 2 . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| PATIENTS AND METHODS : Formalin embedded histopathological samples from forty patients with progressive metastatic breast cancer were reanalyzed to determine HER 2 expression by immunohistochemistry ( ICH ) and chromogenic in situ hybridization ( CISH ) . ^^^ HER 2 expression was positive in 35 % of patients by ICH and in 25 % by CISH . ^^^ With CISH determined HER 2 , the corresponding p values were 0 . 07 , 0 . 053 , and 0 . 002 , respectively . ^^^ CONCLUSION : CISH and ICH determination of HER 2 correlate similarly to hormone receptor status and Bcl 2 expression in breast cancer . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| Therefore , our specific objective was to establish a chromogenic in situ hybridization ( CISH ) assay for the detection of HER 2 amplification on a cohort of 173 archival invasive breast carcinomas . ^^^ HER 2 overexpression was found by IHC in 24 . 3 % ; HER 2 amplification was found by CISH in 19 . 1 % and by dPCR in 9 . 2 % of the tumors . ^^^ Four of 13 tumors with weak HER 2 overexpression ( score +2 ) were negative with both FISH and CISH . ^^^ The current study showed that CISH represents a practical and simple assay for evaluating HER 2 gene amplification in archival material , offering a promising alternative to IHC or FISH for the routine diagnostic setting . . ^^^ |
|
| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| Polymerase chain reaction ( PCR ) and chromogenic in situ hybridization ( CISH ) represent upcoming methods for assessing HER 2 gene amplification . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| Amplification of HER 2 and topoII was assayed using a novel chromogenic in situ hybridisation ( CISH ) method . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| If a probe for chromosome 17 ( now available for CISH ) is used in cases of questionable HER 2 / neu amplification , CISH seems to be as accurate and more practical than FISH . . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| Paraffin embedded tissue sections of the same tumors were analyzed by immunohistochemical staining applying the monoclonal HER 2 / neu antibody TAB 250 ( n=124 ) and by chromogenic in situ hybridization ( CISH ) ( n=73 ) . ^^^ In addition , p 185 concentration measured by ELISA was correlated with the degree of HER 2 / neu gene amplification determined by CISH . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| Determination of HER 2 gene amplification by chromogenic in situ hybridization ( CISH ) in archival breast carcinoma . ^^^ PURPOSE : To compare the efficacy of chromogenic in situ hybridization ( CISH ( TM ) ) with fluorescence in situ ( FISH ) hybridization and immunohistochemistry ( IHC ) in determination of the HER 2 status in human breast cancer . ^^^ MATERIALS AND METHODS : HER 2 gene amplification was determined on formalin fixed paraffin embedded ( FFPE ) sections of 62 invasive breast cancers by FISH and followed by CISH using a digoxigenin ( DIG ) labeled HER 2 DNA probe generated by Subtraction Probe Technology ( SPT ( TM ) ) , and a biotin labeled chromosome 17 centromeric ( chr . 17cen ) probe . ^^^ After in situ hybridization , the HER 2 probe was detected with fluorescein ( FITC ) anti DIG for FISH , followed by peroxidase anti FITC and diaminobenzidine ( DAB ) for CISH . ^^^ For CISH application , HER 2 gene copies or chromosome 17 centromeres and morphology of cells were easily visualized simultaneously with a 40x objective under bright field microscope in hematoxylin counterstained sections . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| Agreement between chromogenic in situ hybridisation ( CISH ) and FISH in the determination of HER 2 status in breast cancer . ^^^ CISH was performed in 79 breast carcinomas for which the HER 2 status was previously determined by IHC and FISH . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| Immunohistochemical ( IHC ) HER 2 / neu protein overexpression was found in 17 . 6 % of canine mammary gland carcinomas , a percentage similar to that observed in human breast carcinoma , but there was no gene amplification by chromogenic in situ hybridization ( CISH ) . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| From this series , we selected 106 cases ( 32 G 1 , 36 G 2 , and 38 G 3 ) in which HER 2 / neu gene analysis , using chromogenic in situ hybridization ( CISH ) , was successful . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| PATIENTS AND METHODS : The Her 2 / neu oncogene amplification and protein over expression were compared in 79 screen detected and 39 interval breast carcinomas , using a novel and well documented chromogenic in situ hybridization ( CISH ) method and immuhistochemistry . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| HER 2 chromogenic in situ hybridization ( CISH ) and immunohistochemistry ( IHC ) were compared to the FISH results . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| HER 2 gene amplification assay : is CISH an alternative to FISH ? ] . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| Chromogenic in situ hybridization ( CISH ) : a novel alternative in screening archival breast cancer tissue samples for HER 2 / neu status . ^^^ METHODS : We assessed Her 2 / neu alteration using CISH on formalin fixed paraffin embedded primary invasive ductal carcinoma tumors in which IHC ( CB 11 antibody ) had previously been performed , and we compared the results with IHC . ^^^ We also compared age at diagnosis and tumor histologic grade with IHC and CISH Her 2 / neu . ^^^ The agreement between 3+ IHC and CISH amplified cases as well as between all IHC and CISH Her 2 / neu negative cases was 100 % , and the concordance on all positive cases was 72 . 50 % , with an overall agreement of 86 . 25 % . ^^^ Although we noted Her 2 / neu positivity more in premenopausal women , the age at diagnosis was not significantly associated with IHC or CISH results . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : The purpose of this study was to assess the clinical relevance of HER 2 amplification by a novel chromogenic in situ hybridisation ( CISH ) technique in patients with primary breast cancer and to determine its relationship with other prognostic markers . ^^^ Expression of HER 2 in tumour tissue samples was assessed by immunohistochemistry ( IHC ) and CISH . ^^^ HER 2 expression , as assessed by CISH , is an independent marker for unfavourable prognosis in primary breast cancers . . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| METHODS : Tissue microarray constructed of 401 serous ovarian carcinomas was examined by chromogenic in situ hybridization ( CISH ) using probe for ERBB 2 gene and by immunohistochemistry using CB 11 monoclonal antibody against ERBB 2 protein . ^^^ CONCLUSION : ERBB 2 amplification positive tumors identified by CISH constitute a subgroup of serous ovarian carcinomas associated with aberrant p 53 , negative progesterone receptor status and aggressive behavior , a suitable group for testing the effect of trastuzumab in clinical trials . . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| Paraffin embedded sections from 61 breast carcinomas were tested for Her 2 amplification by immunohistochemistry ( IHC ) and CISH . ^^^ CISH detected low level Her 2 amplification in 4 of 9 of these cases . ^^^ When compared with FISH , CISH was more sensitive than IHC for detecting low levels of Her 2 gene amplification . ^^^ Moreover , excellent concordance was found between FISH and CISH , supporting the conclusion that the CISH assay for Her 2 gene amplification provides an accurate , effective , and practical alternative to FISH . . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| Chromogenic in situ hybridization ( CISH ) , in which the DNA probe is detected with an immunohistochemistry ( IHC ) like peroxidase reaction , has been recently developed for the assessment of HER 2 / neu status in formalin fixed breast cancer specimens . ^^^ We have applied the technique of dual colour CISH using HER 2 / neu and chromosome 17 centromere probes in 27 cytological smears , and these cytological samples were obtained from scrapings of fresh breast tumours . ^^^ We also investigated HER 2 / neu amplification and protein overexpression in the corresponding surgical tissues by CISH and IHC using the monoclonal antibody CB 11 . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| In a retrospective study on a cohort of 173 archival invasive breast carcinomas a chromogenic in situ hybridisation ( CISH ) assay for the detection of HER 2 amplification was established . ^^^ HER 2 overexpression was found by IHC in 24 . 3 % , HER 2 amplification by CISH in 19 . 1 % and by dPCR in 9 . 2 % of the tumours . ^^^ The current study showed that CISH offers an ideal approach that allows detection of HER 2 amplification in the context of morphology , whereas the major drawback of dPCR is the impracticability of tissue differentiation of invasive and non invasive carcinoma . . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| PATIENTS AND METHODS : The frequency of HER 2 / neu and Topoisomerase IIalpha gene amplification was studied in adenocarcinomas of the stomach ( n=131 ) and the gastroesophageal junction ( n=100 ) by chromogenic in situ hybridization ( CISH ) . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| We compared chromogenic in situ hybridization ( CISH ) with fluorescence in situ hybridization ( FISH ) for assessing HER 2 / neu gene amplification using tissue microarrays ( TMAs ) made from formalin fixed , paraffin embedded tissue blocks from 113 cases of invasive breast carcinoma . ^^^ All 22 cases of borderline ( ratio , 2 . 0 2 . 5 ) or low level ( ratio , 2 . 6 3 . 9 ) amplification by FISH also showed HER 2 gene amplification by CISH . ^^^ CISH is as sensitive as FISH in detecting borderline and low level HER 2 amplification . ^^^ CISH performs as well as FISH in the analysis of HER 2 gene amplification in breast cancer and might have advantages in certain situations . . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| The authors conclude that CISH is a practical alternative to FISH as a confirmatory tool for HER 2 gene amplification status . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| The pros and cons of those two tests and the other upcoming methods for assessing HER 2 status ( with a focus on chromogenic in situ hybridization CISH recently approved by European Commission ) are described in this manuscript . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| The purpose of the present study was to compare chromogenic in situ hybridization ( CISH ) with immunohistochemistry ( IHC ) in determination of HER 2 status , in metastatic breast cancer patients screened for the clinical study of chemotherapy + / herceptin . ^^^ HER 2 was amplified by CISH in 32 cases ( 57 % ) while 33 ( 59 % ) were HER 2 positive by IHC . ^^^ A concordance between HER 2 status determined by CISH and IHC was noted in 43 of 56 cases ( 77 % ; P = 0 . 00008 ) . ^^^ It is suggested that CISH could be considered as a useful additional method to IHC in determining HER 2 status in breast cancer patients , with a recommendation for testing not only the 2+ but also the 1+ subgroup of patients . . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| Comparison of liquid based cytology and histology for the evaluation of HER 2 status using immunostaining and CISH in breast carcinoma . ^^^ AIMS : To correlate immunocytochemical ( ICC ) expression of HER 2 and gene amplification determined by chromogenic in situ hybridisation ( CISH ) using liquid based cytology ( LBC ) with immunohistochemistry ( IHC ) and CISH using histological samples of the same breast carcinomas . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| Her 2 gene amplification was evaluated by chromogenic in situ hybridization ( CISH ) . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| Recently , chromogenic in situ hybridization ( CISH ) , in which HER 2 is detected by a peroxidase reaction and the gene copies are determined by regular bright field microscopy , has emerged as a potential alternative to FISH . ^^^ We found that agreement among the three pathologists on the CISH determined HER 2 status was achieved in 73 cases ( 91 % ) , all of which had results matching the corresponding FISH results : 54 nonamplified and 19 amplified . ^^^ We conclude that , in general , HER 2 status can be reliably assessed by CISH . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : The purpose of this study was to evaluate HER 2 gene status in relation to chromosome 17 polysomy with the chromogenic in situ hybridization ( CISH ) technique and to compare the results with those of immunohistochemistry ( IHC ) . ^^^ HER 2 gene status was evaluated on paraffin sections with the CISH technique using a digoxigenin labeled DNA probe . ^^^ CONCLUSIONS : CISH is an accurate and practical technique for the evaluation of both HER 2 gene and chromosome 17 status and its application is considered necessary especially for the clarification of the 2+ results of IHC . . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| In addition to fluorescence in situ hybridization ( FISH ) and immunohistochemical stain ( HercepTest ) , chromogenic in situ hybridization ( CISH ) has been shown to be a sensitive and specific method to determine the Her 2 / neu status of surgical specimens . ^^^ The effectiveness of CISH in detecting the Her 2 / neu oncogene in cytologic specimens has not been well documented . ^^^ Both CISH and FISH were performed on each case using a digoxigenin labeled Her 2 DNA probe for CISH ( Zymed ) and both Her 2 and chromosome 17 probes for FISH ( Vysis ) . ^^^ In conclusion , our preliminary data suggest that CISH is a useful technique to determine Her 2 / neu oncogene status in cytologic specimens . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| Role of the FISH and CISH techniques in the analysis of HER 2 ] . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| Routinely processed paraffin embedded tissue was investigated for HER 2 gene amplification using CISH and FISH . ^^^ HER 2 gene amplification detection results using CISH and FISH showed a concordance of 100 % . ^^^ Our results indicate that CISH could provide an accurate and practical alternative to FISH for the clinical diagnosis of HER 2 oncogene amplification in bladder cancer . . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| HER 2 expression can be determined through IHC , FISH , CISH and ELISA among other tests , with reported positivity frequencies of overexpression varying from 1 . 8 % to 76 % . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| METHODS : EGFR and HER 2 gene amplification was examined in atypical adenomatous hyperplasia ( AAH ) , bronchioloalveolar carcinoma ( BAC ) , and adenocarcinoma with mixed subtypes ( MX ) by chromogenic in situ hybridisation ( CISH ) , and protein expression was examined by immunohistochemistry using paraffin wax embedded tissues . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| Role of chromogenic in situ hybridization ( CISH ) in the evaluation of HER 2 status in breast carcinoma : comparison with immunohistochemistry and FISH . ^^^ We report our experience with Chromogenic in Situ Hybridization ( CISH ) for the evaluation of HER 2 amplification on 55 cases of formalin fixed , paraffin embedded invasive breast carcinomas of different histology . ^^^ CISH is a useful tool to evaluate breast cancer HER 2 status that can be easily implemented in a laboratory of surgical pathology . . ^^^ |
|
| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| HER 2 gene amplification by chromogenic in situ hybridisation ( CISH ) compared with fluorescence in situ hybridisation ( FISH ) in breast cancer A study of two hundred cases . ^^^ The purpose of the study was to compare two methods used to analyse HER 2 gene amplification ( fluorescence in situ hybridisation ( FISH ) and chromogenic in situ hybridisation ( CISH ) ) , and determine the accuracy of the antibodies CB 11 and HercepTest for immunohistochemical detection of HER 2 overexpression from archival breast cancer tissue . ^^^ Our results show that CISH is a highly accurate , reproducible and practical technique to determine HER 2 gene amplification . ^^^ The performance of tissue macroarrays to test HER 2 status by IHC , FISH and CISH has demonstrated to be an available and effective method to study large series of tumours . . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| Immunohistochemistry ( IHC ) and fluorescent in situ hybridization ( FISH ) techniques , used to detect HER 2 expression in the tumor , are improving constantly and other parallel techniques such as chromogenic in situ hybridization ( CISH ) are emerging . sHER 2 concentrations can be measured using ELISA techniques , which can be automated . ^^^ |
|
| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| Chromogenic in situ hybridization ( CISH ) , which uses an enzymatic reaction to detect the hybridized DNA probe , is a new alternative to fluorescence in situ hybridization ( FISH ) for the assessment of HER 2 oncogene amplification status in breast cancer . ^^^ The present paper presents an efficient protocol for dual colour CISH ( dc CISH ) based on the co hybridization of probes to the HER 2 oncogene and chromosome 17 centromere . ^^^ It is concluded that dual colour CISH , which is a new alternative to FISH enables the assessment of copy number ratio ( HER 2 / 17 centromere ) in conjunction with proper histopathological evaluation and the ease of bright field microscopy . . ^^^ |
|
| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| Immunohistochemistry assay for HER 2 protein expression was performed according to the HercepTest protocol , and HER 2 gene amplification was examined with the Spot light CISH ( chromogenic in situ hybridization ) Detection kit . ^^^ |
|
| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| AIMS : The objective of this study was to evaluate the accuracy , ease of use and reproducibility of chromogenic in situ hybridisation ( CISH ) for HER 2 testing by studying its inter laboratory concordance in five Australian pathology laboratories . ^^^ METHODS : The HER 2 status of 49 breast cancers was determined by CISH twice in two different laboratories . ^^^ CONCLUSIONS : These findings demonstrate that CISH is a robust test to assess HER 2 status in breast cancer and therefore is an important addition to the HER 2 testing algorithm . . ^^^ |
|
| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| Immunohistochemistry ( IHC ) and fluorescent in situ hybridization ( FISH ) techniques , which are used to detect HER 2 expression in the tumor , are improving constantly , and other parallel techniques such as chromogenic in situ hybridization ( CISH ) are starting to emerge . sHER 2 concentrations can be measured using ELISA techniques , which can be automated . ^^^ |
|
| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| PATIENTS AND METHODS : Sixteen patients with HER 2 / neu overexpressing tumors ( 15 were 3+ by immunohistochemistry ( IHC ) and one 2+ by IHC and positive by the chromogenic in situ hybridization ( CISH ) test ) were included in the study . ^^^ All samples were assayed for HER 2 by IHC and by the CISH test . ^^^ RESULTS : Six out of the 16 ( 37 % ) ( `` altered HER 2 / neu status ' ' ) newly developed metastatic lesions lost their HER 2 / neu overexpression and scored 0 or +1 by IHC or negative on the CISH test , while in the remaining cases ( 10 / 16 , 62 . 5 % ) ( `` conserved HER 2 / neu status ' ' ) , the HER 2 / neu status was unchanged ( +3 by IHC or a positive CISH test ) . ^^^ CONCLUSION : These data suggest that , for most patients with metastatic breast cancer treated with trastuzumab , the HER 2 / neu expression as measured by IHC and / or CISH in newly developed metastatic lesions was unchanged . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| HER 2 / neu overexpression and gene amplification was examined with semiquantitative standardized immunohistochemical staining , chromogenic in situ hybridization ( CISH ) , and fluorescence in situ hybridization ( FISH ) in 182 gastric cancer patients who underwent curative surgery at the Kangbuk Samsung Hospital . ^^^ HER 2 / neu gene amplification was detected in seven patients by CISH and FISH . ^^^ |
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| Interacting proteins: Q71V34 and P04626 |
Pubmed |
SVM Score :0.0 |
| In view of this , in the present study , we analyzed the usefulness of CISH on formalin fixed , paraffin embedded ( FFPE ) BC specimens and investigated whether CISH can be a valid technique in the determination of HER 2 status for fine needle aspirates ( FNAs ) processed by liquid based cytology . ^^^ |
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