| Interacting proteins: Q14451 and P04626 |
Pubmed |
SVM Score :0.94399547 |
| Grb 7 binds to erbB 2 and may be involved in cell signaling pathways that promote the formation of metastases and inflammatory responses . 0.94399547^^^ |
|
| Interacting proteins: Q14451 and P04626 |
Pubmed |
SVM Score :0.0 |
| Consistent with previous reports , we also observed that the steroidogenic acute regulatory protein related gene , MLN 64 , and growth factor receptor bound protein 7 were coexpressed with ERBB 2 . ^^^ |
|
| Interacting proteins: Q14451 and P04626 |
Pubmed |
SVM Score :0.0 |
| Growth factor receptor bound protein 7 ( Grb 7 ) is an adaptor protein that is co overexpressed and forms a tight complex with the ErbB 2 receptor in a number of breast tumours and breast cancer cell lines . ^^^ |
|
| Interacting proteins: Q14451 and P04626 |
Pubmed |
SVM Score :0.0 |
| Grb 10 maps to mouse chromosome 11 very close to the EGF Receptor which is remarkably similar to Grb 7 that maps near the EGF Receptor related HER 2 receptor . ^^^ |
|
| Interacting proteins: Q14451 and P04626 |
Pubmed |
SVM Score :0.0 |
| The SH 2 domain protein GRB 7 is co amplified , overexpressed and in a tight complex with HER 2 in breast cancer . ^^^ We find that GRB 7 is amplified in concert with HER 2 in several breast cancer cell lines and that GRB 7 is overexpressed in both cell lines and breast tumors . ^^^ GRB 7 , through its SH 2 domain , binds tightly to HER 2 such that a large fraction of the tyrosine phosphorylated HER 2 in SKBR 3 cells is bound to GRB 7 . ^^^ The fact that GRB 7 and HER 2 are both overexpressed and bound tightly together suggests that this basic signaling pathway is greatly amplified in certain breast cancers . . ^^^ |
|
| Interacting proteins: Q14451 and P04626 |
Pubmed |
SVM Score :0.0 |
| The Grb 7 protein was recently identified as a substrate of the epidermal growth factor receptor and related Her2 / erbB 2 receptor linked tyrosine kinase activity . ^^^ |
|
| Interacting proteins: Q14451 and P04626 |
Pubmed |
SVM Score :0.0 |
| Structural determinants of the interaction between the erbB 2 receptor and the Src homology 2 domain of Grb 7 . ^^^ The Src homology 2 ( SH 2 ) domain containing protein Grb 7 and the erbB 2 receptor tyrosine kinase are overexpressed in a subset of human breast cancers . ^^^ Whereas the Grb 7 SH2 domain binds strongly to erbB 2 , the SH 2 domain of Grb 14 , a protein closely related to Grb 7 , does not . ^^^ We have investigated the preferred binding site of Grb 7 within the erbB 2 intracellular domain and the SH 2 domain residues that determine the high affinity of Grb 7 compared with Grb 14 for this site . ^^^ Phosphopeptide competition and site directed mutagenesis revealed that Tyr 1139 of erbB 2 is the major binding site for the Grb 7 SH2 domain , indicating an overlap in binding specificity between the Grb 7 and Grb 2 SH2 domains . ^^^ |
|
| Interacting proteins: Q14451 and P04626 |
Pubmed |
SVM Score :0.0 |
| Molecular cloning of human GRB 7 co amplified with CAB 1 and c ERBB 2 in primary gastric cancer . ^^^ We report here the structure of human GRB 7 cDNA , and we report that the amounts of mRNA for c ERBB 2 , CAB 1 , and GRB 7 were elevated in concordance with the amplification . . ^^^ |
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| Interacting proteins: Q14451 and P04626 |
Pubmed |
SVM Score :0.0 |
| The functions of Grb 7 and Grb 10 are unknown , but Grb 7 is overexpressed in certain breast cancers , where it is bound to the growth factor receptor HER 2 , while Grb 10 has been implicated in insulin signaling . ^^^ |
|
| Interacting proteins: Q14451 and P04626 |
Pubmed |
SVM Score :0.0 |
| Four new genes ( A 39 , C 51 , CAB 1 , and GRB 7 ) coamplified with c ERBB 2 were isolated from the enriched cDNA library . ^^^ CAB 1 , GRB 7 , and c ERBB 2 were overexpressed in gastric and esophageal cancer cells in correspondence with the amplification . ^^^ |
|
| Interacting proteins: Q14451 and P04626 |
Pubmed |
SVM Score :0.0 |
| Co expression of erbB 2 with erbB 3 point mutants was used to map Grb 7 binding sites . ^^^ |
|
| Interacting proteins: Q14451 and P04626 |
Pubmed |
SVM Score :0.0 |
| During the course of studies on the structural characterization of the amplification units , we isolated four cDNA clones , A 39 , GRB 7 , C 51 and CAB 1 with corresponding genes localized on the c ERBB 2 locus . ^^^ |
|
| Interacting proteins: Q14451 and P04626 |
Pubmed |
SVM Score :0.0 |
| As expected , these included ERBB 2 as well as the GRB 7 and MLN 64 genes previously shown to be coamplified with ERBB 2 . ^^^ Amplicon mapping by fluorescence in situ hybridization revealed a minimal common region of amplification containing four highly expressed genes , ERBB 2 , GRB 7 , MLN 64 , and an uncharacterized EST 48582 . ^^^ |
|
| Interacting proteins: Q14451 and P04626 |
Pubmed |
SVM Score :0.0 |
| Grb 7 is an adapter type signaling protein , which is recruited via its SH 2 domain to a variety of receptor tyrosine kinases ( RTKs ) , including ErbB 2 and ErbB 3 . ^^^ |
|
| Interacting proteins: Q14451 and P04626 |
Pubmed |
SVM Score :0.0 |
| GRB 7 is tightly linked to ERBB 2 and is coamplified and coexpressed with this gene in several cancer types . ^^^ Interestingly , the expression levels of ERBB 2 were not elevated in the TGCTs , suggesting that GRB 7 might be the target for the increased DNA copy number in TGCTs . ^^^ |
|
| Interacting proteins: Q14451 and P04626 |
Pubmed |
SVM Score :0.0 |
| Moreover , the structure suggests the mechanism by which the Grb 7 SH2 domain binds selectively to pTyr 1139 ( pYVNQ ) in Her 2 , which along with Grb 7 is co amplified in human breast cancers . . ^^^ |
|
| Interacting proteins: Q14451 and P04626 |
Pubmed |
SVM Score :0.0 |
| Human MGC 9753 gene , consisting of eight exons , were clustered with PPP1R1B , STARD 3 , TCAP , PNMT , ERBB 2 , MGC 14832 and GRB 7 genes within the 120 kb region . ^^^ PPP1R1B , STARD 3 , MGC 9753 , ERBB 2 and GRB 7 genes are co amplified in several cases of gastric cancer . ^^^ |
|
| Interacting proteins: Q14451 and P04626 |
Pubmed |
SVM Score :0.0 |
| Coimmunoprecipitation experiments also indicated that Grb 7 and NIK could be simultaneously recruited into signaling complexes of all known EGF / heregulin receptors , including EGFR , ErbB 2 , ErbB 3 , and ErbB 4 . ^^^ |
|
| Interacting proteins: Q14451 and P04626 |
Pubmed |
SVM Score :0.0 |
| In addition to these differences , these analyses revealed that in contrast to the mouse mammary tumor virus based Neu model , the endogenous promoter tumors expressed elevated levels of two genes ( Grb 7 and Cab 1 ) that are closely linked to ErbB 2 and often coamplified in noninvasive ductal carcinoma in situ . ^^^ |
|
| Interacting proteins: Q14451 and P04626 |
Pubmed |
SVM Score :0.0 |
| Solution structure of the human Grb 7 SH2 domain / erbB2 peptide complex and structural basis for Grb 7 binding to ErbB 2 . ^^^ |
|
| Interacting proteins: Q14451 and P04626 |
Pubmed |
SVM Score :0.0 |
| Coamplification and coexpression of GRB 7 and ERBB 2 is found in high grade intraepithelial neoplasia and in invasive Barrett ' s carcinoma . ^^^ GRB 7 is localized in close proximity to the ERBB 2 gene within an amplicon previously identified in Barrett ' s adenocarcinoma ( BCA ) . ^^^ We evaluated gene amplification and mRNA expression of GRB 7 and ERBB 2 in Barrett ' s carcinoma and its associated precursor lesions to assess their possible role in Barrett ' s malignant transformation . ^^^ Copy number and expression levels were analyzed by Q PCR ( GRB 7 , ERBB 2 ) and QRT PCR ( GRB 7 , ERBB 2 ) using TaqMan technology and fluorescence in situ hybridization ( ERBB 2 ) in a series of 24 laser microdissected samples of Barrett ' s carcinomas and 32 samples of associated premalignant lesions . ^^^ Parallel analysis of gene copy number changes and expression levels demonstrated that GRB 7 and ERBB 2 displayed concomitant elevated expression levels and increased copy numbers in 32 % of Barrett ' s carcinomas . ^^^ |
|
| Interacting proteins: Q14451 and P04626 |
Pubmed |
SVM Score :0.0 |
| Transcripts from genes that flank ERBB 2 including GRB 7 , a protein linked to metastasis in esophageal cancer , also showed high levels of expression . ^^^ |
|
| Interacting proteins: Q14451 and P04626 |
Pubmed |
SVM Score :0.0 |
| Activation of ErbB 2 by heregulin beta 1 decreases the pool of Grb 7 associated to membranes . ^^^ |
|
| Interacting proteins: Q14451 and P04626 |
Pubmed |
SVM Score :0.0 |
| An evident ERBB 2 cluster was observed due to the marked over expression of the ERBB 2 gene and GRB 7 and PPARBP in this patient material ) . ^^^ |
|
| Interacting proteins: Q14451 and P04626 |
Pubmed |
SVM Score :0.0 |
| Expression of oncogenic Erbb 2 ( NeuNT ) under control of the endogenous Erbb 2 promoter results in frequent ( 85 % ) amplification at the Erbb 2 locus with striking structural similarity to the human amplicon , resulting in overexpression of at least two of the genes , Erbb 2 and Grb 7 . ^^^ |
|
| Interacting proteins: Q14451 and P04626 |
Pubmed |
SVM Score :0.0 |
| Expression of HER 2 and the coamplified genes GRB 7 and MLN 64 in human breast cancer : quantitative real time reverse transcription PCR as a diagnostic alternative to immunohistochemistry and fluorescence in situ hybridization . ^^^ Expression of GRB 7 , MLN 64 , and p 21 , genes coregulated with HER 2 , was also quantified with quantitative RT PCR and correlated with the overall survival ( OS ) and disease free survival ( DFS ) individually and in combination with HER 2 . ^^^ The combination of HER 2 with MLN 64 , but not with GRB 7 or p 21 , enhanced the prognostic power for the DFS ( P < 0 . 00005 ) and OS ( P < 0 . 0008 ) . ^^^ |
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| Interacting proteins: Q14451 and P04626 |
Pubmed |
SVM Score :0.0 |
| These receptors can bind the adaptor molecule GRB 7 encoded by a gene adjacent to ERBB 2 at 17q12 , a region also frequently gained in TGCTs . ^^^ |
|
| Interacting proteins: Q14451 and P04626 |
Pubmed |
SVM Score :0.0 |
| Higher expression correlated with shorter DRFS for the HER 2 adaptor GRB 7 and the macrophage marker CD 68 . ^^^ |
|
| Interacting proteins: Q14451 and P04626 |
Pubmed |
SVM Score :0.0 |
| Notably , targeted knockdown of either GRB 7 or STARD 3 also leads to decreased cell proliferation and cell cycle progression , albeit to a lesser extent compared with ERBB 2 knockdown . ^^^ |
|
| Interacting proteins: Q14451 and P04626 |
Pubmed |
SVM Score :0.0 |
| This 168 kb region contains the following genes : PPP1R1B / DARPP 32 , STARD 3 , TCAP , PNMT , PERLD 1 , ERBB 2 , C17orf37 , and GRB 7 as well as the first two exons of ZNFN1A3 . ^^^ |
|
| Interacting proteins: Q14451 and P04626 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14451 and P04626 |
Pubmed |
SVM Score :0.0 |
| NA |
|