Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.66670982
Serum starved NIH 3T3 cells expressing an epidermal growth factor receptor ( EGF R ) / neu construct encoding a hybrid receptor protein were stimulated with EGF and the activation of the neu tyrosine kinase and stimulation of growth factor inducible genes were followed at the mRNA , protein , and activity levels , and compared to the corresponding responses in the neu proto oncogene and oncogene expressing cells . 0.66670982^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.82452671
Despite the extensive similarity between the c erbB 2 protein and EGF receptor , neither EGF nor TGF alpha bound to the c erbB 2 protein . 0.82452671^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.91076826
We show that in various cells ErbB 2 can form heterodimers with both EGF receptor ( ErbB 1 ) and NDF receptors ( ErbB 3 and ErbB 4 ) , suggesting that it may affect the action of heterologous ligands without the involvement of a direct ErbB 2 ligand . 0.91076826^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.54907613
These data suggest that EGF imparts signals that involve activation of ErbB 2 and are associated with ErbB 2 PI 3 kinase activation . 0.54907613^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.63207705
We conclude that in 3T3 F442A cells , 1 ) the GH induced decrease in ErbB 2 tyrosine phosphorylation correlates with MEK1 / mitogen activated protein kinase activity and 2 ) GH antagonizes EGF induced DNA synthesis and cyclin D 1 expression in a pattern consistent with its alteration in ErbB 2 phosphorylation status . . 0.63207705^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.50598272
Cells that expressed ErbB 2 and treated with EGF showed prolonged activation of Raf 1 and MAP kinase compared with cells that expressed low levels of ErbB 2 . 0.50598272^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.55461954
To further dissect the ligand binding selectivity of the ErbB network , we have applied the phage display technique to examine the role of the linear N terminal region in EGF for interaction with ErbB 2 / ErbB 3 heterodimers . 0.55461954^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Neu differentiation factor : a transmembrane glycoprotein containing an EGF domain and an immunoglobulin homology unit . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
In all the specimens examined , HER 2 / neu expression did not seem to be related to EGF binding capacity . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Similar internalization is shown by a chimeric molecule EGFR / erbB 2 in response to EGF . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
In all the specimens examined , HER 2 / neu expression did not seem to be related to EGF binding capacity . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Amplification and overexpression of the EGF receptor and c erbB 2 proto oncogenes in human stomach cancer . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Two growth factors , epidermal growth factor ( EGF ) and insulin , stimulated phosphorylation of the HER 2 protein product . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
EGF receptor and erbB 2 tyrosine kinase domains confer cell specificity for mitogenic signaling . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Direct interaction of a ligand for the erbB 2 oncogene product with the EGF receptor and p185erbB2 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Tyrosine kinase receptor with extensive homology to EGF receptor shares chromosomal location with neu oncogene . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
In homology it is similar to the human EGF receptor and to rat and human neu proteins . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The EGF receptor homolog , ErbB 2 protein , also lacks this motif . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The EGF receptor ( EGFR ) and HER 2 are members of a growth factor receptor family . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Expression of c erbB 2 protein and EGF receptor in hepatitis B , cirrhosis and hepatocellular carcinoma ] . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Northern blotting also revealed the co expression of 5 . 6 kb EGF R mRNA and 4 . 6 kb c erb B 2 mRNA . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
In contrast to EGF receptor and c erbB 2 , we were unable to detect binding of activated c erbB 3 to GRB 2 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
EGF markedly increased ErbB 2 tyrosine phosphorylation in wild type EGFR cells . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The biological responses to HRG were also compared to EGF and to the growth inhibitory anti ErbB 2 antibody , 4D5 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
TPA did not induce the degradation of related receptors ( ErbB 1 , ErbB 2 , and ErbB 3 ) in the EGF receptor family . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Receptor tyrosine kinases ( EGF receptor and c erbB 2 ) have been shown to be important in the pathogenesis of cancer . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
HB EGF binds to NIH 3T3 cells overexpressing either HER 1 or HER 4 alone , but not HER 2 or HER 3 alone . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
In these cancerous tissues , no clear amplification of EGF r and c erb B 2 protein expression was observed . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
These include EGF receptor ( erbB 1 ) and erbB 4 when expressed individually and erbB 2 and erbB 3 when expressed together . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Exogenous TGF alpha activated EGFR , ErbB 2 and MAPK , whereas EGF activated only the EGFR . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The erbB 3 gene encodes a transmembrane protein that is related to the epidermal growth factor ( EGF ) receptor and erbB 2 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Epidermal growth factor ( EGF ) receptor tyrosine kinases include ErbB 1 , ErbB 2 , ErbB 3 and ErbB 4 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Using immunohistochemistry , the expression of the EGF receptor , ERBB 2 , and TP 53 was determined . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
HER 2 activation likely contributes to downstream signaling events and enhances EGF receptor action . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Oestrogen and vitamin D receptor ( VDR ) genotypes and the expression of ErbB 2 and EGF receptor in human rectal cancers . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Immunohistochemical staining for AX 1 , c erbB 2 , p 53 protein , ER , PR , EGF and IGF were performed by LSAB . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Overexpression of p 21 ras , c erbB 2 , epidermal growth factor ( EGF ) and EGF receptor were obtained . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
BTC induced phosphorylation of all three EGF receptors present on HUVECs : ErbB 2 , ErbB 3 , and ErbB 4 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Gab 1 is required for EGF receptor signaling and the transformation by activated ErbB 2 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
By contrast , EGF activates ErbB 1 / ErbB 1 or ErbB 1 / ErbB 2 dimers and induces cell growth and survival . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The presence of the EGF receptors EGFR , ErbB 2 , and ErbB 3 was confirmed through Western blot analysis of cell lysates . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
BTC induced phosphorylation of all four EGF receptors present on HTASMCs : ErbB 1 , ErbB 2 , ErbB 3 , and ErbB 4 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Signaling via the EGF receptor and HER 2 / neu can activate both ER and the important ER coactivator AIB 1 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Unlike other ErbB proteins , ErbB 2 binds no known EGF like ligand . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Two members of the EGF receptor family , HER 2 and HER 3 , act as key oncogenes in breast cancer cells . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
EGF receptor signalling involving the erbB 2 and PI3K pathways plays a role in mediating these events in Capan 1 cells . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Cholesterol dictates the freedom of EGF receptors and HER 2 in the plane of the membrane . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
HER 2 is a member of the EGF receptor family , which participates in cell growth and proliferation . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
HER 2 is a member of the EGF receptor family , which participates in cell growth and proliferation . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Each contains a transmembrane subunit with two epidermal growth factor ( EGF ) like domains that act as ligand for ErbB 2 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
In addition , the novel growth factor stimulated phosphorylation of the EGF receptor and erbB 2 receptor . ^^^ We conclude that this TGF alpha like ( gp 30 ) polypeptide is a novel growth factor with agonistic activity for both EGF and erbB 2 receptors . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Abnormalities of the EGF receptor and / or the related ERBB 2 receptor occur in a significant proportion of cases of human breast cancer and are important influences in the behaviour of this tumour type . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
In contrast , the proliferative activity ( ploidy and S phase , Thymidine Labeling Index , antibody Ki 67 ) , cathepsin D , thymidine kinase , EGF receptors , several genes including oncogene HER 2 / neu , are recently developed prognostic factors whose significance needs to be confirmed by further studies . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Overexpression of many growth factors / receptors , such as EGF , TGF alpha , EGF receptor and ERBB 2 , and reduction of type 1 receptor for TGF beta may be linked with new prognostic factors of gastric carcinomas . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The neu protein ( p 185 ) has a molecular weight of 185 , 000 Daltons and , like the EGF receptor , possesses tyrosine kinase activity . neu is activated in chemically induced rat neuro / glioblastomas by substitution of valine 664 with glutamic acid within the transmembrane domain . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The expressions of epidermal growth factors ( EGF ) , epidermal growth factor receptors ( EGFR ) , and the c erbB 2 oncoprotein were immunohistochemically examined in 25 cases of human pancreatic carcinoma and epineoplastic pancreatitis and in 10 non cancerous / non inflammatory pancreatic tissues . ^^^ The positive rates of EGF , EGFR , and the c erbB 2 oncoprotein in cancer tissues were 72 % , 36 % , and 28 % , respectively . ^^^ In the EGF , EGFR , and c erbB 2 positive cancer tissues , some stromal cells , that is fibroblasts and endothelial cells , were also positive . ^^^ These results suggest that the coexpression of EGF and EGFR and the expression of the c erbB 2 oncoprotein are related to the existence of the invasion of human pancreatic cancer . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The erbB 2 protein content varied dramatically depending upon cell density and upon the presence of epidermal growth factor ( EGF ) in the culture medium . ^^^ The addition of EGF to the culture medium caused an increase in the phosphoamino acid content and an increase in the turnover of the erbB 2 protein . ^^^ Cell fractionation experiments were performed , and a shift in the cellular localization of the erbB 2 protein towards the lysosomal compartment in EGF treated HC 11 cells was found . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Immunohistochemical studies on oncogene products ( EGF R , c erbB 2 ) and growth factors ( EGF , TGF alpha ) in human breast cancer : their relationship to oestrogen receptor status , histological grade , mitotic index and nodal status . ^^^ In this investigation , 83 human mammary carcinomas were examined for the expression of oestrogen receptor ( ER ) , epidermal growth factor receptor ( EGF R ) , epidermal growth factor ( EGF ) , transforming growth factor alpha ( TGF alpha ) , c erbB 2 , histological grade , mitotic index and nodal status , all of which are reportedly prognostically significant factors ( Bloom and Richardson 1957 ; Baak et al . 1985 ; Wright et al . 1989 ) . ^^^ ER expression was biochemically recognized in 43 . 4 % of mammary carcinomas , and EGF R , EGF , TGF alpha and c erbB 2 were histochemically recognized in 25 . 3 , 14 . 5 , 27 . 7 and 18 . 0 % of mammary carcinomas examined respectively , using conventional sections of buffered formalin fixed , paraffin embedded tissue and monoclonal or polyclonal antibodies . ^^^ There were significant relationships between negative ER and positive EGF R or TGF alpha ; positive EGF R and TGF alpha ; positive EGF R and c erbB 2 ; and positive c erbB 2 and TGF alpha . ^^^ Therefore , the present investigation indicates that the negative ER , single expression of c erbB 2 and co expression of EGF R and TGF alpha are important markers which contribute indirectly to prognosis , which reconfirms previous findings on the former two while adding the new finding that immunohistochemical demonstration of expression of EGF R and TGF alpha may provide useful information for selecting the appropriate treatment . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
We show that in response to EGF , wild type EGFR and various EGFR mutants were able to undergo heterodimerization with HER 2 . ^^^ Addition of EGF to transfected cells co expressing HER 2 with a kinase negative point mutant of EGFR ( K721A ) stimulated heterodimer formation , tyrosine phosphorylation of K721A and HER 2 , and tyrosine phosphorylation of one of their known substrates , phospholipase C gamma . ^^^ However , the binding of EGF to transfected cells co expressing HER 2 together with another EGFR mutant CD 533 ( a deletion mutant lacking most of the cytoplasmic domain of EGFR ) caused heterodimerization and inhibition of tyrosine kinase activity . ^^^ It appears therefore that EGF induced heterodimerization of EGFR and HER 2 can promote either stimulatory or inhibitory influences on kinase activity . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
In this work , we have used Xenopus oocyte maturation as a read out for examining the ability of the neu tyrosine kinase ( p185neu ) to participate with the epidermal growth factor ( EGF ) receptor in a common signal transduction pathway . ^^^ We find that unlike the case for the EGF receptor , which elicits EGF dependent maturation of these oocytes as reflected by their germinal vesicle breakdown ( GVBD ) , neither the normal neu tyrosine kinase ( p185val664 ) nor the oncogenic form of neu ( p185glu664 ) are able to effectively trigger this maturation event . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
To address these issues , we utilized a chimeric molecule encompassing the extracellular domain of the epidermal growth factor ( EGF ) receptor ( EGFR ) fused to the transmembrane and intracellular domains of the erbB 2 product . ^^^ In this EGFR / erbB 2 chimera , erbB 2 kinase activity is regulated by EGF binding . ^^^ An EGFR / erbB 2 mutant bearing multiple Tyr Phe substitutions at erbB 2 autophosphorylation sites ( EGFR / erbB 2 5P ) displayed markedly reduced phosphotyrosine content following EGF stimulation in comparison with the non mutated chimera . ^^^ When expressed in NR 6 cells , the EGFR / erbB 2 5P mutant was unable to deliver a sizeable mitogenic signal when activated by EGF at physiological levels . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The rat neu oncogene product is a member of the epidermal growth factor ( EGF ) receptor subgroup of the superfamily of growth factor receptor tyrosine kinases . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Recent data suggest that the mechanism by which HER 2 mediates transformation requires the interaction of the epidermal growth factor ( EGF ) receptor . ^^^ To test whether overexpression of normal human HER 2 can transform cells independently of the EGF receptor , we have introduced multiple copies of HER 2 into the EGF receptor negative cell line , NR 6 , and have performed assays for both transformation and tumorigenicity . ^^^ This study provides clear evidence that HER 2 mediated transformation can be achieved independently of the EGF receptor . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The family currently comprises three closely related members : the epidermal growth factor ( EGF ) receptor , c erbB 2 and c erbB 3 , all of which show abnormalities of expression in various human tumours . ^^^ The family of factors related to EGF has also expanded recently and now includes transforming growth factor alpha , heparin binding EGF , amphiregulin , cripto and heregulin , as well as several other potential ligands for the c erbB 2 2 receptor . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Interactions between the epidermal growth factor ( EGF ) receptor and the HER 2 protein have been described . ^^^ The aim of this study was to elucidate the effects of EGF on HER 2 expression . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The epidermal growth factor ( EGF ) receptor ( EGFR ) and the erbB 2 gene product , gp185erbB 2 , exhibit distinct abilities to stimulate mitogenesis in different target cells . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Proliferating cell nuclear antigen in breast lesions : correlation of c erbB 2 oncoprotein and EGF receptor and its clinicopathological significance in breast cancer . ^^^ A high index was correlated with c erbB 2 and epidermal growth factor ( EGF ) receptor membrane reactivity , worsening histological grade , poor survival and disease free survival . ^^^ The expression of c erbB 2 and EGF receptor was associated with poor survival and disease free survival in primary breast cancer patients . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
This mechanism could function early in the progression of breast cancer . c erbB 2 and EGF receptor overexpression and amplification , when they occur , appear to render tumors even more malignant and of especially poor prognosis . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
There is evidence for a potential autocrine loop involving overexpression of the epidermal growth factor ( EGF ) receptor and its ligands , as well as overexpression of the erbB 2 receptor . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Several oncoproteins ( c myc , c Ha ras , c erbB 2 ) and growth factors / receptors ( EGF , EGF R , TGF alpha ) were immunostained . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NC3H10 and TC 3H10 respectively , but we do not know whether the neu oncogene expression can be induced by EGF . ^^^ The result indicated that EGF had a obvious induction effect on neu oncogene expression in these cells . ^^^ Thus , the expression of both erbB 1 / EGFR gene and erbB 2 / neu oncogene can be induced by EGF . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Expression of the c erbB 3 protein in normal human adult and fetal tissues . c erbB 3 is a member of the type 1 family of growth factor receptors which includes the epidermal growth factor ( EGF ) receptor and c erbB 2 . ^^^ Whereas for EGF receptor and c erbB 2 the expression patterns in normal tissues are well documented , there is currently little information available about the sites of c erbB 3 expression . ^^^ The c erbB 3 protein was identified in cells of the gastrointestinal , reproductive , respiratory and urinary tracts as well as the skin , endocrine and nervous system in a distribution distinctly different from that observed for EGF receptor and c erbB 2 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Detectable levels of c myc , Her 2 , c myb , K ras and EGF receptor mRNA were found in most cell lines , whereas only 1 / 11 and 2 / 11 cell lines were positive for N myc and c sis message , respectively . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Analysis of the mutants suggests that transformation of NRK cells by the 5 fms , 5 erbB , activated erbB 2 , 5 ras , 5 fos , 5 mos , 5 fes , 5 src , SV 40 large T , polyomavirus middle T , and human papillomavirus type 16 E 6 , E 7 oncogenes is mediated by the EGF / PDGF signal cascade . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The expression of the EGF receptor , c erbB 2 and PDGF receptor proteins has been studied in a series of human brain tumour biopsies and cell lines . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The erbB 2 gene product , gp185erbB 2 , unlike the structurally related epidermal growth factor ( EGF ) receptor ( EGFR ) , exhibits constitutive kinase and transforming activity . ^^^ We used a chimeric EGFR / erbB 2 expression vector to compare the mitogenic signaling pathway of the erbB 2 kinase with that of the EGFR , at similar levels of expression , in response to EGF stimulation . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The neu protooncogene ( also called HER 2 and c erbB 2 ) encodes a cell surface tyrosine kinase structurally related to the receptor for the epidermal growth factor ( EGF ) . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
DER encodes a transmembrane cell surface receptor with a cytoplasmic protein tyrosine kinase domain , and resembles the vertebrate genes that encode the EGF receptor and the neu protein . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Three different receptor tyrosine kinases , epidermal growth factor ( EGF ) , c erbB 2 / neu , and platelet derived growth factor ( PDGF ) receptors , have been found to be present in the mouse mammary epithelial cell line HC 11 . ^^^ The results show that activation of the structurally related EGF and c erbB 2 / neu receptors , but not the PDGF receptor , allows the HC 11 cells to subsequently respond optimally to lactogenic hormones . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Among the novel tyrphostins examined we found inhibitors which discriminate between the highly homologous EGF receptor kinase ( HER 1 ) and ErbB2 / neu kinase ( HER 2 ) . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
In this review 1 summarize the experimental data in favor of the notion that control of epidermal growth factor ( EGF ) receptor ( R ) and / or c erbB 2 protooncogene expression by specific autocrine growth factors and certain classical endocrine hormones serves as a transducer of extracellular signals that ultimately lead to growth responses in breast carcinoma cells . 1 summarize some new results on the role of epidermal growth factor ( EGF ) , transforming growth factor ( TGF ) alpha , and TGF beta in the control of EGF R protooncogene expression in human breast carcinoma cells . ^^^ Furthermore , the data embracing the hypothesis that the growth actions of hormone receptors that are homologous to the 5 erbA oncogene ( estrogens , progesterone , thyroid hormones , retinoic acid , and vitamin D ) are mediated , in part , by modulating EGF R and / or c erbB 2 protooncogene transcription are reviewed . ^^^ Finally , 1 develop the theme that cooperation of certain c erb A related , c erbB 2 and / or EGF R gene products contribute to the uncontrolled growth of human mammary carcinoma cells . ^^^ From the evidence reviewed , one can infer that elucidation of the molecular control of EGF R / c erbB 2 gene expression by c erbA related gene products may lead to both a better understanding of breast carcinogenesis and a new therapeutic approach directed at controlling the transcriptional responses of EGF R / c erbB 2 genes . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The HER 2 protooncogene encodes a 185 kDa transmembrane protein ( p185HER2 ) with extensive homology to the epidermal growth factor ( EGF ) receptor . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Neu oncogene encodes a cell surface receptor similar to the EGF receptor and is known to be activated by a point mutation of DNA that encodes the transmembrane domain of the neu protein ( p 185 ) . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Using MDP CM , the presence of a 25 kDa polypeptide distinct from EGF , PDGF , FGF , IGF , TGF alpha and TGF beta and TNF alpha , could be demonstrated by decorating a Western blot with soluble NEU and anti NEU antibodies . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Mutant p 53 , EGF receptor and c erbB 2 expression in human breast cancer . p 53 expression was studied in 111 primary breast cancers with a monoclonal antibody ( PAb 240 ) that reacts with an epitope in mutant p 53 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Down regulation of the PDGF receptors and their mRNAs was also observed after EGF treatment of cells expressing a chimeric EGF receptor / neu receptor , where the neu tyrosine kinase is activated by EGF binding . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Because of its substantial homology with the EGF receptor , erbB 2 has long been assumed to encode a growth factor receptor , although until recently definitive identification of ligand ( s ) has remained elusive . ^^^ TGF alpha and EGF are incapable of directly inducing phosphorylation of erbB 2 . ^^^ The ability to induce phosphorylation of erbB 2 is not inhibited by an anti EGF receptor blocking antibody . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Tyrosine phosphorylated proteins were similar but not identical in epidermal growth factor ( EGF ) stimulated cells expressing the human EGF receptor ( EGFR ) or a chimeric EGFR / neu receptor but differed from phosphotyrosyl proteins constitutively expressed in neu oncogene transformed cells . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
This technically straight forward change in methodology resulted in chromogenic immunocytochemical assays for Her 2 and EGF receptors that were 1 2 orders of magnitude more sensitive while still fully utilizing the diagnostic resolving power of light microscopy . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
In addition to the EGF R , B 11 recognized two unidentified soluble proteins present in the cytoplasm of the SKBR 3 cell line but different from the c erb B 2 oncoprotein expressed by these cells . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The EGF independence of S 2 was accompanied by an overexpression of the mRNAs for epidermal growth factor receptor ( EGF R ) , transforming growth factor alpha , and c erb B 2 as compared to the EGF dependent subline ( S 1 ) . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The expression of mRNA of EGF receptor gene and ERBB 2 by TMK 1 cells was not changed by erbstatin treatment , whereas that of c src was slightly decreased . ^^^ Erbstatin inhibited protein kinase activity of EGF receptor , p185ERBB2 and pp60c src in TMK 1 cells . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
There are abnormalities in the structure and / or function of several oncogenes and growth factors in human pancreatic cancer , notably the EGF receptor and its ligand TGF alpha , c erb B 2 proto oncogene , Ki ras oncogene and the tumour suppressor gene p 53 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Regulation by EGF is maintained in an overexpressed chimeric EGFR / neu receptor tyrosine kinase . ^^^ A chimeric construct encoding the human EGF receptor extracellular domain fused to the tyrosine kinase domain of the rat neu cDNA was expressed under the transcriptional control of the Moloney murine leukemia virus LTR promoter . ^^^ The ligand activated cells became morphologically transformed and grew in agar in the presence of EGF and TGF beta as efficiently as did the ligand independent neu oncogene transformed cells . ^^^ Our results establish similarities between the signal pathways of the EGF receptor and the neu tyrosine kinase . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The neu oncogene product , p185neu , is a tyrosine kinase receptor with structural similarity to the epidermal growth factor ( EGF ) receptor . ^^^ We have recently described that coexpression of EGF receptors and high levels of normal p185c neu lead to transformation of rodent fibroblasts . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The Drosophila epidermal growth factor receptor homolog ( DER ) displays sequence similarity to both the epidermal growth factor ( EGF ) receptor and the neu vertebrate proteins . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
These results suggest that the known structural similarity of the neu gene product to the EGF receptor is also reflected in a functional similarity by which the mutationally activated neu protein can replace the ligand activated EGF receptor . ^^^ These results also suggest that the ability of ras and neu transformed cells to escape the effect of the inhibitory serum activity is a nonautocrine property distinct from the acquisition of EGF autonomy . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The c erbB 2 proto oncogene encodes a 190 k Mr protein representing a putative growth factor receptor with considerable homology to the EGF receptor . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The protein product of the neu protooncogene , p 185 , is a tyrosine kinase with a high degree of sequence homology to the epidermal growth factor ( EGF ) receptor . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The erbB 1 and erbB 2 protooncogenes encode homologous membrane receptors that respectively bind epidermal growth factor ( EGF ) and a still incompletely characterized ligand . ^^^ Binding of EGF to its receptor is known to increase tyrosine phosphorylation of the erbB 2 / neu receptor in tumor cells . ^^^ Chemical cross linking of 125I labeled EGF revealed that the radiolabeled EGF receptor coimmunoprecipitates with the erbB 2 / neu receptor . ^^^ The formation of the latter species is absolutely dependent on the presence of EGF receptor and thus appears to represent a heterodimer of the erbB 1 and erbB 2 receptors . ^^^ Moreover , analysis of living SKBR 3 cells suggested that most of the EGF induced transregulation of the erbB 2 / neu receptor is due to receptor heterodimerization . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
For example , the epidermal growth factor ( EGF ) responsive normal NOG 8 mouse and human MCF 10A mammary epithelial cell lines can be transformed with either a point mutated c Ha ras protooncogene or with a normal or point mutated c neu ( erbB 2 ) protooncogene . ^^^ In ras transformed NOG 8 and MCF 10A cells but not in neu transformed cells there is a loss in or an attenuated response to the mitogenic effects of EGF . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
K 12 cells expressed characteristic double affinity EGF receptors , as well as p 185 ( a functionally and structurally related protein , product of the neu gene ) on their surface . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
This is substantiated by the facts that EGF and TGF alpha act as autocrine growth factors and then induce the expression of mRNAs for multi growth factors and their receptors ( EGF , TGF alpha , EGFR , ERBB 2 , PDGF ) . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Overexpression of the Epidermal Growth Factor ( EGF ) Receptor gene and the related erbB 2 gene has been identified in human cancers derived from a variety of tissues . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Amplification of c erbB 2 and EGF receptor genes has been observed in many metastatic sites of gastric carcinomas regardless of histological type . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Both EGF and TGF alpha stimulated EGFR phosphorylation , EGF and TGF alpha induced FOS , MYC and ERBB 2 oncogene expression . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The products from some of the activated proto oncogenes ( c sis , c erbB 2 ) are sequences homologous to a certain growth factor ( PDGF ) and a receptor ( EGF ) identified in lung cancer . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The c erb B 2 oncogene encodes a 190 kD transmembrane growth factor receptor which is closely related to the EGF receptor and has been found to be amplified and overexpressed in a number of human adenocarcinomas , particularly of the breast . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The hypothesis is supported by the fact that many cancer cells have some dysfunction in gap junctional intercellular communication , many tumor promoting chemicals and several oncogenes ( i . e . , ras , src , mos , neu , but not myc ) reduce gap junctional intercellular communication , and several growth factors ( i . e . , EGF , TGF beta , bovine pituitary extract ) inhibit gap junction function . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
However the changes in S EGF were small and the decrease of U EGF in patients with NEP was remarkable ( U EGF of without complications : 22 . 7 + / 2 . 5 , with RET and / or NEU and without NEP : 24 . 5 + / 4 . 2 , all with NEP : 17 . 6 + / 3 . 2 ng / mg creatinine , mean + / SE ) . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Examples of such genes include the erbB / EGF receptor and erbB 2 , which encode structurally related receptor proteins and are often amplified and / or overexpressed in epithelial malignancies . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Overexpression of two receptors , c erbB 2 and EGF receptor , have also been associated with poor prognosis in the clinical disease . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Using a specific antibody to the c terminal portion of the human c erbB 2 gene product , we have found that gp 190 is the human c erbB 2 gene product which is structurally closely related to the epidermal growth factor ( EGF ) receptor . ^^^ Since monoclonal antibody specific for the EGF receptor abolished both EGF binding to its receptor and tyrosine phosphorylation of the c erbB 2 gene product , we have concluded that activation of EGF receptor tyrosine kinase activity upon EGF binding leads to the phosphorylation of the c erbB 2 gene product on its tyrosine residues . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Although similar structural mutation of the EGF receptor has not yet been found in human tumours , aberrant overexpression of both EGF receptors and c erbB 2 , a closely related putative receptor , have been found to occur in squamous cell carcinomas and glial tumours , and mammary carcinomas respectively . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Class 1 includes the EGF receptor and the neu proto oncogene , Class 2 includes the insulin and insulin like growth factor 1 ( IGF 1 ) receptors , and Class 3 the platelet derived growth factor ( PDGF ) and the colony stimulating factor 1 ( CSF 1 ) receptors . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The neu oncogene , characterized by Weinberg and colleagues , is a transforming gene found in ethylnitrosourea induced rat neuro / glioblastomas ; its human proto oncogene homologue has been termed erbB 2 or HER 2 because of its close homology with the epidermal growth factor receptor ( EGF R ) gene ( c erbB 1 ) . ^^^ A chimeric EGF R neu proto oncogene allows EGF to regulate neu tyrosine kinase and cell transformation . ^^^ We constructed a vector for expression of a chimeric cDNA and hybrid protein consisting of the EGF R extracellular , transmembrane and protein kinase C substrate domains linked to the intracellular tyrosine kinase and carboxyl terminal domain of the rat neu cDNA . ^^^ Immunofluorescence and immunoprecipitation using neu and EGF receptor specific antibodies demonstrated a correctly oriented and positioned chimeric EGF R neu protein of the expected apparent mol . wt on the surface of these cells . ^^^ EGF or TGF alpha induced tyrosine phosphorylation of the chimeric receptor protein , stimulated DNA synthesis of EGF R neu expressing cells and led to a transformed cell morphology and growth in soft agar . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
For this reason , we replaced the HER 2 extracellular domain with the closely related ligand binding domain sequences of the epidermal growth factor ( EGF ) receptor , and examined the ligand induced biological signalling potential of this chimeric HER 1 2 protein . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Synergistic interaction of p185c neu and the EGF receptor leads to transformation of rodent fibroblasts . ^^^ Several groups have recently demonstrated EGF induced , EGFR mediated phosphorylation of p185c neu . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
These effects were specific for ras because neu transformed NOG 8 cells grew in soft agar at a comparable level to NOG 8 SR 2 cells yet did not show any increase in TGF alpha production or change in EGF receptor expression . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Two clones of NIH3T3 fibroblasts , NEN 37 and NEN 7 , overexpressing chimeric EGF / neu receptors ( 3 10 10 ( 5 ) and 1 10 10 ( 6 ) receptors / cell , respectively ) , were treated with EGF in order to identify the array of intracellular signals generated after activation of the neu proto oncogene product . ^^^ In addition to the stimulation of the neu tyrosine kinase , previously reported by Lehvaslaiho et al . ( EMBO J . , 8 , 159 166 , 1989 ) , EGF ( 10 ( 9 ) 10 ( 8 ) M ) was found to induce marked increases of both [ Ca2+ ] 1 and plasma membrane potential ( investigated by the fura 2 and bis oxonol techniques ) which , in their initial phase , were only marginally dependent on the presence of Ca2+ in the incubation medium . ^^^ It is concluded that the intracellular signals identified here may play a limited role in the neu induced cell proliferation , but are possibly involved in the acquisition of the tumoral phenotype typically expressed by the EGF treated NEN 7 cells . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The human c erbB 2 gene encodes a growth factor receptor like protein that is highly homologous to the epidermal growth factor ( EGF ) receptor . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Similarly , the neu product becomes phosphorylated on tyrosine residues following treatment of cells with EGF , although the neu protein does not bind EGF itself ( King et al . , 1988 ; Stern and Kamps , 1988 ) . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Receptor downregulation and DNA synthesis are modulated by EGF and TPA in cells expressing an EGFR / neu chimera . ^^^ EGF was used to stimulate a chimeric receptor consisting of the epidermal growth factor receptor ( EGFR ) extracellular , transmembrane , and protein kinase C substrate domains linked to the intracellular tyrosine kinase and carboxyl terminal domains of the rat neu protein in NIH / 3T3 cells . ^^^ EGF bound to the cells was rapidly internalized in a complex with the EGFR / neu protein , as shown by loss of EGF binding and EGFR antigens from the cell surface . ^^^ These results show that the chimeric EGFR / neu receptor undergoes typical downregulation upon ligand binding and TPA pretreatment and is capable of transducing an EGF induced mitogenic signal . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
There are two genes related to the viral erbB gene in the human genome . c erbB 1 is the same as the gene for the epithelial growth factor ( EGF ) receptor , and c erbB 2 encodes a receptor like protein very similar to , but distinct from , the EGF receptor . ^^^ Hybridisation analysis of DNA from 101 fresh human malignant tumours showed that the c erbB 2 gene was amplified in 5 of 63 adenocarcinomas and none of 38 other types of tumours , whereas the c erbB 1 / EGF receptor gene was amplified only in 1 of 8 squamous cell carcinomas . ^^^ Thus , the protein products of the amplified c erbB 2 gene may have a role in the evolution of adenocarcinomas , as does the EGF receptor in some squamous cell carcinomas . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The neu oncogene was originally identified in cell lines derived from rat neuroectodermal tumors . neu is related to but distinct from the c erbB gene , which encodes the epidermal growth factor ( EGF ) receptor . neu encodes a protein , designated p 185 , that is serologically related to the EGF receptor . ^^^ Identification of the normal homolog of p 185 encoded by the neu proto oncogene enabled us to compare the product of the neu proto oncogene with the mutated version specified by the neu oncogene and with the EGF receptor . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Treatment of human adenocarcinoma MKN 7 cells with epidermal growth factor ( EGF ) or phorbol tetradecanoate acetate ( TPA ) stimulated phosphorylation of the c erbB 2 gene product . ^^^ Tryptic phosphopeptide mapping analysis suggested that treatments with EGF and TPA induced phosphorylation of many common sites in the c erbB 2 gene product . ^^^ However , in contrast to TPA , EGF increased the phosphorylation of the c erbB 2 protein in cells whose protein kinase C had been down regulated by long term pretreatment with TPA , suggesting that EGF and TPA induce phosphorylation by different mechanisms . ^^^ Since the c erbB 2 gene product did not show detectable EGF binding activity , phosphorylation of tyrosine of the c erbB 2 gene product might be catalyzed directly by the EGF receptor kinase that was activated by EGF . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Oncogenic rodent p 185 and the human p 185 homologue ERBB 2 that is overexpressed in human breast tumor cells also can be shown to become phosphorylated on tyrosine residues by the action of EGF . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Egf binding to its receptor triggers a rapid tyrosine phosphorylation of the erbB 2 protein in the mammary tumor cell line SK BR 3 . ^^^ The epidermal growth factor receptor ( EGF R ) and the erbB 2 proto oncogene product protein are closely related by their structural homology and their shared enzymatic activity as autophosphorylating tyrosine kinases . ^^^ We show that in mammary tumor cells ( SK BR 3 ) EGF causes a rapid increase in tyrosine phosphorylation of the erbB 2 protein . ^^^ Phosphorylation of erbB 2 does not occur in cells lacking the EGF R ( MDA MB 453 ) . ^^^ Phosphorylation of erbB 2 in SK BR 3 cells is blocked if EGF is prevented from interacting with its receptor by specific monoclonal antibodies . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The role of the neu oncogene product in cell transformation and normal development . p185neu , the protein product of the neu gene , is a tyrosine kinase receptor with structural similarity to the epidermal growth factor ( EGF ) receptor . ^^^ We have defined : 1 ) stage and tissue specific expression patterns of the neu gene product in developing tissues ; 2 ) p185neu phosphorylation and the regulation of p185neu tyrosine kinase activity by EGF . 3 ) Synergistic interactions of cellular rat p185neu and EGF receptor leading to cell transformation ; 4 ) structural and functional differences of normal and oncogenic p185neu . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
We were also able to identify the c erbB 2 gene that seems to code for a EGF receptor like protein with a domain for tyrosine kinase . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The neu gene : an erbB homologous gene distinct from and unlinked to the gene encoding the EGF receptor . ^^^ It was concluded that the neu gene is a distinct novel gene , as it is not coamplified with sequences encoding the EGF receptor in the genome of the A 431 tumor line and it maps to human chromosome 17 . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Thus , we conclude that clone lambda 107 represents a 5 erbB related gene ( c erbB 2 ) that is distinct from the EGF receptor gene . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
A novel 5 erb B related gene , c erb B 2 , which has been identified in the human genome , maps to human chromosome 17 at q 21 ( ref . 40 ) , and seems to encode a polypeptide with a kinase domain that is highly homologous with , but distinct from , that of the epidermal growth factor ( EGF ) receptor . ^^^ Its sequence shows that the c erb B 2 gene encodes a possible receptor protein and allows an analysis of the similarity of the protein to the EGF receptor and the neu product . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Although the c erbB 2 protein was predicted to encode a protein very similar to epidermal growth factor ( EGF ) receptor , EGF did not stimulate this kinase activity either in vivo or in vitro . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Overexpression of the EGF receptor related proto oncogene erbB 2 in human mammary tumor cell lines by different molecular mechanisms . ^^^ Amplification of the erbB / EGF receptor and a structurally related gene , designated erbB 2 , have previously been detected in a variety of human tumors . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The amino acid sequences surrounding the three identified phosphorylation sites are highly conserved in the EGF receptor and the protein products of the 5 erb B and neu oncogenes . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Antibodies to one of the peptides , which showed no detectable cross reaction with human , rat or monkey EGF receptor , were used to examine the expression of the c erbB 2 protein on a variety of cultured cell types . ^^^ Eleven transformed , 1 non established and 2 immortalized cell types were examined by immunoprecipitation for their level of expression of the c erbB 2 protein and of the EGF receptor . ^^^ The numbers of EGF receptors varied widely between different cell lines , whereas the level of the c erbB 2 protein , which was found on all of the cell types examined , was more constant . ^^^ The c erbB 2 protein was also present on normal human epithelial cells but in some cases with a different distribution to that of the EGF receptor . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The c erbB 2 gene is a 5 erbB related proto oncogene which encodes a protein similar to but distinct from the epidermal growth factor ( EGF ) receptor . ^^^ In addition , the c erbB 2 protein is suggested to be a substrate for EGF receptor tyrosine kinase , since EGF binding to the EGF receptor rapidly induced phosphorylation of the c erbB 2 protein on its tyrosine residue . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Alignment of the sequences , the identification of conserved residue patterns and secondary structure predictions indicate that the extra cellular regions of the human and Drosophila epidermal growth factor ( EGF ) , c erb B 2 and human insulin receptors each contain two large , homologous domains ( L ) which are probably comprised of at least four short alpha helices followed by turns of conserved length and beta strands . ^^^ In the human and Drosophila EGF and c erb B 2 receptors these homologous domains are each followed by a series of smaller cystine rich domains ( S ) to give a gene duplicated structure of L1S11S12S13L2S21S22S23 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Previous work has shown that the neu gene is related to , but distinct from , the gene encoding the EGF receptor ( c erb B ) . ^^^ We found that 50 % of the predicted amino acids of neu and the EGF receptor are identical ; greater than 80 % of the amino acids in the tyrosine kinase domain are identical . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The neu oncogene bears homology to erb B and the tumour antigen , p 185 , is serologically related to the epidermal growth factor ( EGF ) receptor . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The expression of the neu oncogene product was investigated immunohistochemically in 36 cases of benign prostatic hyperplasia ( BPH ) and seven cases of adenocarcinoma of prostate ( CaP ) . c neu oncogene encodes a transmembrane growth factor receptor that has partial structural homology with EGF receptor , and is overexpressed and amplified in a number of human tumors , specially , breast cancers . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
We now show that mutant Neu proteins resulting from a point mutation at the ATP binding site ( N 757 ) or cytoplasmic domain deletions ( N691stop ) are still able to undergo EGF induced heterodimerization with EGFR . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
We have previously reported that oestrogen inhibits erbB 2 mRNA and protein expression in breast cancer cells , while epidermal growth factor ( EGF ) treatment has been shown to decrease p185erbB 2 levels in normal mouse mammary epithelial cells . ^^^ In the present work , we studied the effect of oestrogen and EGF on erbB 2 expression in oestrogen responsive breast cancer cells . ^^^ Oestrogens , but not EGF , concomitantly down regulated erbB 2 mRNA . ^^^ Furthermore , the transcriptional activity of a 219 bp proximal fragment of the human erbB 2 promoter was repressed by oestrogens , whereas it was enhanced by EGF . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
ErbB 2 becomes rapidly phosphorylated and activated following treatment of many cell lines with epidermal growth factor ( EGF ) or Neu differentiation factor ( NDF ) . ^^^ However , these factors do not directly bind ErbB 2 , and its activation is likely to be mediated via transmodulation by other members of the type I / EGF receptor ( EGFR ) related family of receptor tyrosine kinases . ^^^ The precise role of ErbB 2 in the transduction of the signals elicited by EGF and NDF is unclear . ^^^ We conclude that in T47D cells , ErbB 2 is a major NDF signal transducer and a potentiator of the EGF signal . ^^^ ErbB 2 becomes rapidly phosphorylated and activated following treatment of many cell lines with epidermal growth factor ( EGF ) or Neu differentiation factor ( NDF ) . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The product of the c erbB 2 protooncogene ( p 185 ) is a member of the EGF receptor family of transmembrane tyrosine kinases . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Transforming growth factor alpha ( TGF alpha ) is a potent mitogenic factor which acts by binding to the epidermal growth factor ( EGF ) . c erbB 2 is a member of the EGF receptor family and is known to be associated with cellular growth and differentiation . ^^^ Twenty six out of 36 ( 72 . 22 % ) frozen BPH tissues exhibited moderate to strong staining for immunoreactive EGF receptor in the cell membrane . c erbB 2 oncoprotein was localized in 35 out of 39 cases ( 89 . 74 % ) with the intensity of staining being variable . ^^^ All nine cases positive for TGF alpha were also positive for both EGF receptor and c erbB 2 protein . ^^^ Immunohistochemical studies indicate the expression of TGF alpha , EGF receptor and c erbB 2 protein in BPH tissues . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Moreover , in established cell lines expressing elevated levels of EGFR , EGF stimulation results in transphosphorylation of Neu and formation of complexes between c Src and tyrosine phosphorylated Neu . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
There was no significant association between AR , HRG , and CR 1 expression and nodal status , EGF receptor , or c erbB 2 protooncogene expression in these tumors . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The c erbB 2 proto oncogene codes for a 185 kd putative growth factor receptor that is highly homologous to but distinct from the epidermal growth factor ( EGF ) receptor . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Like heregulin , epidermal growth factor ( EGF ) also stimulate the tyrosine phosphorylation of both Neu and ErbB 3 . ^^^ Thus the formation of a stable ErbB 3 p85 complex in PC 12 cells is a unique outcome of heregulin signaling that correlates with the differences in cell morphology induced by the activated EGF receptor and the Neu tyrosine kinase . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
A single point mutation , Glu 627 > Val , equivalent to the activating mutation in the Neu oncogene , was inserted in the transmembrane domain of the human epidermal growth factor ( EGF ) receptor . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The ability of AR to elicit in vivo phosphorylation of the EGF receptor ( EGFR ) and p185erbB2 was studied in four human epithelial cell lines which expressed either or both of the receptor tyrosine kinases . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Transfection of SFME cells with oncogenes ( ras , neu , SV 40 T antigen ) results in cells that grow in serum supplemented medium and no longer require EGF for survival . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Here we report that this newly discovered MAPK is physically associated with the EGF receptor in A 431 cells and with the related receptor / tyrosine kinase HER 2 ( encoded by c neu ) in enzyme preparations obtained from Wilm ' s tumors . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The results showed that metastatic lesions expressed high levels of ErbB 2 , nidogen and aromatase but unchanged levels of EGF receptors and HGF . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The extent of inhibition correlated with both receptor saturation and affinity of different NDF isoforms , and it was abolished upon overexpression of either EGF receptor or ErbB 2 . ^^^ Neu differentiation factor inhibits EGF binding . ^^^ Neu differentiation factor ( NDF , or heregulin ) and epidermal growth factor ( EGF ) are structurally related proteins that bind to distinct members of the ErbB family of receptor tyrosine kinases . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Grb 10 maps to mouse chromosome 11 very close to the EGF Receptor which is remarkably similar to Grb 7 that maps near the EGF Receptor related HER 2 receptor . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Expression of the ERBB 2 protein was present in 54 % ( 20 / 37 ) but immunoreactivity was much weaker than for EGF receptor and in most cases barely detectable by Western analysis and immunocytochemistry . ^^^ Our data from immunohistochemistry indicate that ERBB 2 expression in GBM is closely correlated with EGF receptor levels and is therefore not useful as an independent prognostic parameter . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
We have analyzed the effects of Neu differentiation factor ( NDF ) / heregulin , a newly described activating ligand for erbB 2 and other members of the epidermal growth factor ( EGF ) receptor family , on cell growth and the expression of milk proteins in HC 11 cells . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Gene expression of growth factors including epidermal growth factor ( EGF ) , transforming growth factor alpha ( TGF alpha ) , epidermal growth factor receptor ( EGFR ) , oncogenes such as c myc , N ras , c erbB 2 and tumor suppressor gene P 53 were studied in 4 human lung cancer cell lines using Northern blot technique . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Evidence is also presented that suggests that an EGF R related protein , ErbB 2 , may be involved in similar Src mediated interactions . ^^^ Overexpression of Src , EGF R , and / or ErbB 2 in breast and colorectal tumor cells suggests the potential that such interactions may contribute to the transformed phenotype of these carcinomas . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Many human tumors over express erbB 2 and EGF receptors . ^^^ We have used recombinant DNA technology to produce single chain antibody exotoxin A ( scFv ETA ) fusion proteins which specifically bind the erbB 2 and EGF receptors . ^^^ Stimulation of SKBR 3 cells and HCII RI # 11 mouse mammary epithelial cells expressing the human erbB 2 with EGF led to an increase in scFv ( FRP 5 ) ETA activity , showing that the EGF induced activation of erbB 2 can potentiate the action of the erbB 2 directed toxin . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Primary and metastatic ovarian cystadenocarcinomas , carcinomas of low malignant potential ( borderline tumors ) , benign ovarian cystadenomas , and normal ovaries were compared for immunoperoxidase detection of the ligands epidermal growth factor ( EGF ) , transforming growth factor alpha ( TGF alpha ) , amphiregulin ( AR ) , cripto , and the receptors , epidermal growth factor receptor ( EGF R ) , and c erbB 2 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Evaluation of biomarkers in breast and prostate cancer . p 53 , p185erbB 2 , and epidermal growth factor ( EGF ) receptor are well characterized biomarkers in invasive adenocarcinoma of the breast and in ductal carcinoma in situ . erbB 2 Protein ( p185erbB 2 ) must be identified clearly on cytoplasmic membranes while cytoplasmic expression is ignored for breast cancers to be classified as overexpressing p185erbB 2 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Since NDF , HER 2 / neu , and HER 3 are present in skin , and other EGF family members can influence wound keratinocytes in vivo , we investigated whether NDF would stimulate epidermal migration and proliferation in a rabbit ear model of excisional wound repair . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Clinical significance of epidermal growth factor ( EGF ) , EGF receptor , and c erbB 2 in human gastric cancer . ^^^ The role of EGF , the EGF receptor , and c erbB 2 in human gastric cancer is reviewed on the basis of several reports , which have been mainly oriented toward their clinical significance . ^^^ Selective blockade of the EGF receptor and c erbB 2 from their ligands with monoclonal antibodies ( MoAbs ) inhibits the growth of human gastric cancer xenografts . ^^^ These MoAbs may therefore be effective antitumor agents against gastric cancer showing overexpression of EGF receptors or c erbB 2 . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The epidermal growth factor ( EGF ) and erbB 2 receptors are structurally related membrane bound tyrosine kinases . ^^^ When cells containing chimeric receptors composed of the EGF R extracellular domain and intracellular domain of erbB 2 were heat stressed , 125I EGF bound to the receptors , but did not stimulate receptor autophosphorylation . ^^^ The decline in EGF stimulated chimeric erbB 2 receptor autophosphorylation is dependent on the length of heat shock , with nearly 100 % of the kinase activity lost after 60 min at 46 degrees C . ^^^ The loss of chimeric receptor erbB 2 kinase activity is not due to degradation of receptor protein , nor is it attributable to a specific transmembrane domain from either the EGF or erbB 2 receptors . ^^^ Insertion of the erbB 2 tyrosine kinase domain into the EGF R confers heat stress sensitivity to the resultant chimeric receptor . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The effect of various growth modulatory agents including estrogen ( E 2 ) , progesterone ( Pg ) , retinoic acid ( RA ) , epidermal growth factor ( EGF ) , insulin ( Ins ) , prolactin ( Prl ) , 12 O tetradecanolyl phorbol 13 acetate ( TPA ) and dibutyryl 3 ' : 5 ' cyclic AMP ( cAMP ) on c erbB 2 promoter activity , RNA and protein expression have been examined . ^^^ The growth stimulators E 2 and EGF both reduced the level of erbB 2 protein . ^^^ However , while E 2 clearly repressed erbB 2 transcription , in the case of EGF , neither mRNA nor transcription were decreased . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
MCF 10A cells transformed by either an activated human c Ha ras protooncogene ( MCF 10A ras ) or by overexpression of a nonactivated rat c neu gene ( MCF 10A neu ) exhibited a 35 % reduction in the response to AR in ADG when compared to MCF 10A cells , but AR was still as potent as EGF in these transformants . ^^^ A 20 mer phosphorothioate antisense deoxyoligonucleotide complementary to the 5 ' sequence of AR mRNA was able to significantly reduce the levels of endogenous AR protein and to inhibit the EGF stimulated ADG and anchorage independent growth of MCF 10A ras and MCF 10A neu cells . ^^^ These data suggest that AR may function as an EGF dependent autocrine growth factor in mammary epithelial cells that have been transformed by either a point mutated c Ha ras or c neu . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
In this study , gene expression of TGF alpha ( a ligand of EGF receptor ) , TGF beta 1 and c erbB 2 oncogene were studied . ^^^ C erbB 2 oncogene ( a homology of EGF receptor ) mRNA was strongly expressed in PC 1 , weakly in PC 2 and was not detectable in PC 3 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
In this study we examined whether epitope tagged HPV E 5 proteins could associate with three different tyrosine kinase containing growth factor receptors : the EGF receptor , the erbB 2 receptor , and the PDGF receptor . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Fortunately , c erbB 2 protein , unlike epidermal growth factor ( EGF ) receptor , survives most fixation procedures used in routine histopathology laboratories . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Expression of EGF , TGF alpha , EGFR and c erbB 2 genes and their gene products in human pancreatic carcinoma cell lines . ^^^ The expression of mRNA for epidermal growth factor ( EGF ) , transforming growth factor alpha ( TGF alpha ) , EGF receptor ( EGFR ) and c erbB 2 genes and the immunoreactivity to these gene products were examined in 3 newly established human pancreatic carcinoma cell lines and their corresponding in vivo tumor lines using the Northern blot technique and the immunohistochemical method . ^^^ All 3 cell lines expressed TGF alpha , EGFR and 2 of the 3 lines expressed EGF and c erbB 2 mRNAs . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Immunocytochemical analyses of EGF , EGFR , and c erbB 2 ( an EGFR related oncoprotein ) were carried out on paraffin embedded sections of 54 pituitary tumors . ^^^ Expression of epidermal growth factor ( EGF ) , its receptor , and related oncoprotein ( erbB 2 ) in human pituitary tumors and response to EGF in vitro . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Expression of the EGF receptor homologue HER 2 was also examined . ^^^ Moreover , HER 2 expression was stimulated by EGF addition in young cells but not in old cells . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
High expression of epidermal growth factor ( EGF ) receptors and erbB 2 has been related to poor prognosis . ^^^ EGF receptors are mainly regulated by transcription , as are some cases of high erbB 2 expression . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
We analyzed by immunohistochemistry 19 typical lung carcinoids for the expression of the epidermal growth factor ( EGF ) , transforming growth factor alpha ( TGF alpha ) , EGF receptor ( EGFr ) , and EGFr related c erbB 2 protein ( p 185 ) . ^^^ Thus , EGFr was coexpressed with its ligands , TGF alpha and EGF , and the receptor encoded by c erbB 2 was detected in carcinoids positive for EGFr and TGF alpha . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The HER 2 ( neu / erb B 2 ) proto oncogene codes for a transmembrane receptor with tyrosine kinase activity and with high homology to the EGF receptor ( HER 1 ) . ^^^ Mitogenic signaling induced by EGF in NIH3T3 cells overexpressing either HER 1 or HER 1 2 ( possessing the HER 2 kinase domain ) was blocked identically by the agents that discriminate between the two in vitro . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Cytosol from EGF treated A 431 human epidermoid carcinoma cells was used as a source of PTPase activity , and tyrosine phosphorylated ErbB 2 , EGF receptor , phospholipase C gamma 1 , and the Ras GTPase activating protein were used as substrates to monitor PTPase activity . ^^^ EGF stimulated PTPase activity that was selective toward these substrates , as it dephosphorylated ErbB 2 and the EGF receptor , but not phospholipase C gamma 1 and the Ras GTPase activating protein . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Immunohistochemical staining for EGF , EGFR , c erbB 2 , p 53 , K ras and PCNA was performed on the formalin fixed , paraffin embedded sections of resected gastric carcinomas . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
In order to evaluate the growth and / or proliferative capabilities of cyst epithelia , epidermal growth factor ( EGF ) , epidermal growth factor receptor ( EGFR ) and c erb B 2 gene product were determined in cyst epithelia , adenoma and adenocarcinoma . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The endocytosis of gp185erbB 2 was studied using chimeric receptors in which the intracellular domain of erbB 2 , or subdomins thereof , was substituted for the corresponding regions of the epidermal growth factor ( EGF ) receptor . ^^^ Chimeric and wild type EGF or erbB 2 receptors were expressed in mouse NIH3T3 or NR 6 fibroblasts and in a human mammary adenocarcinoma cell line , MDAMB 134 . ^^^ The rate of EGF induced internalization for the chimera consisting of the extracellular EGF receptor domain and intracellular erbB 2 domain was reduced three to fourfold compared with the wild type EGF receptor . ^^^ Substitution of the carboxyl terminus of erbB 2 for the corresponding region of the EGF receptor caused a similar decrease of receptor endocytosis , whereas substitution of the erbB 2 tyrosine kinase domain did not affect internalization and down regulation . ^^^ Since the tyrosine kinase of the internalization defective chimeric receptors could be activated by EGF , kinase activity and autophosphorylation of erbB 2 do not appear to be sufficient for a maximum rapid internalization of the chimeric receptors . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Increased or altered expression of growth factor receptors has been described for nerve growth factor ( NGF ) receptor , for PDGF beta receptor and for a truncated form of epidermal growth factor ( EGF ) receptor encoded by the c erb B 2 oncogene . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Gene amplification and overexpression of oncogenes and growth factors / receptors such as c met , K sam , c erbB 2 , EGF and EGF receptor are biological marker of biological malignancy . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The expression of mRNAs for epidermal growth factor ( EGF ) , transforming growth factor alpha ( TGF alpha ) , EGFR , platelet derived growth factor ( PDGF ) A and B chain , PDGF receptor ( PDGFR ) , transforming growth factor beta ( TGF beta ) , erbB 2 and estrogen receptor ( ER ) genes was first examined in 6 human esophageal carcinoma cell lines , 6 xenoplanted and 15 surgically resected esophageal carcinomas . ^^^ The expression of mRNAs for TGF alpha , PDGF A and B chain and the erbB 2 genes was also increased after treatment with EGF . ^^^ TGF alpha increased the accumulation of mRNAs for EGF , TGF alpha , EGFR , PDGF A and B chain and the erbB 2 gene . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
C erbB 2 , EGF receptor , p 53 and PCNA . ^^^ In 216 breast cancer patients , the prognostic value of current biological factors ( c erbB 2 , EGF receptor , p 53 , PCNA proliferative fraction ) was compared with that of conventionally histomorphologic features ( histologic type , histologic grade , tumour size , hormonal receptor status ) . ^^^ Neither c erbB 2 , EGF R , p 53 nor any of the other factors showed any correlation to survival . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Furthermore , neu differentiation factor / heregulin , a ligand that indirectly activates p185erbB 2 by direct binding to erbB 3 receptors , potently stimulated the proliferation of the growth factor dependent H16N 2 cells ( which expressed c erbB 2 and c erbB 3 but not c erbB 4 ) in the absence of both IGF and EGF . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
However , high expression of the EGF receptor protein and p185erbB2 was frequent in RCC and statistically significantly related to higher tumor grades . ^^^ We could demonstrate a close correlation of p185erbB2 overexpression with high EGF receptor levels . ^^^ Our results suggest a synergistic involvement of both EGF receptor and p185erbB2 in the progression of RCC . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
CONCLUSIONS : Determination of the frequency and levels of EGF receptors , HER 2 / neu protein , and cathepsin D in uteri with and without cancer and the availability of reference materials developed in our laboratory , will allow evaluation of their prognostic value in cancers of the uterus . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Four transmembrane tyrosine kinases constitute the ErbB receptor family : the epidermal growth factor ( EGF ) receptor , ErbB 2 , ErbB 3 , and ErbB 4 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
A bivalent single chain antibody toxin specific for ErbB 2 and the EGF receptor . ^^^ ErbB 2 and EGF receptors are often co expressed in human tumors and have been shown to synergize in the transformation of cells in experimental model systems . ^^^ Transactivation of ErbB 2 can occur via ligand induced heterodimerization with EGF receptor or other members of the ErbB family of receptor tyrosine kinases . ^^^ We have previously described the potent anti tumoral activity of the monospecific single chain antibody toxins scFv ( FRP 5 ) ETA and scFv ( 225 ) ETA binding to , respectively , ErbB 2 and the EGF receptor . ^^^ The fusion protein consists of 2 scFv domains specific for ErbB 2 and the EGF receptor linked to a modified Pseudomonas exotoxin A . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
In contrast , all the EGF related factors except amphiregulin were able to induce tyrosine phosphorylation of ErbB 2 . ^^^ While several EGF agonists bind and activate ErbB 1 / EGF receptor , neu differentiation factor ( NDF ) functions as a ligand for ErbB 3 and ErbB 4 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The group of subtype 1 transmembrane tyrosine kinases includes the epidermal growth factor ( EGF ) receptor ( ErbB 1 ) , an orphan receptor ( ErbB 2 ) , and two receptors for the Neu differentiation factor ( NDF / heregulin ) , namely : ErbB 3 and ErbB 4 . ^^^ By using the generated mAbs , we found that the major NDF receptor on mammary epithelial cells is a heterodimer of ErbB 3 with ErbB 2 , whereas an ErbB 1 / ErbB 2 heterodimer , or an ErbB 1 homodimer , is the predominant species that binds EGF . ^^^ Consistent with ErbB 2 being a shared receptor subunit , its tyrosine phosphorylation was increased by both heterologous ligands and it mediated a trans inhibitory effect of NDF on EGF binding . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
These results suggest that with the presence of elevated levels of EGF , TGFalpha , and the oncoprotein receptor c erbB 2 in the membrane of parotid tumors , cell proliferation and activation of the phosphotyrosine signal transduction pathway may involve autocrine stimulation through the expression of high levels of growth factor and receptor in the same tissue . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The ErbB family of transmembrane tyrosine kinases includes the receptor for EGF ( ErbB 1 ) , two receptors for NDF / heregulin ( ErbB 3 and ErbB 4 ) and an orphan receptor ( ErbB 2 ) . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Formalin fixed , paraffinembedded specimens were immunostained for epidermal growth factor ( EGF ) , transforming growth factor alpha ( TGFalpha ) , cripto , EGF receptor ( EGFR ) and c ERBB 2 gene product . ^^^ Of the 68 early colorectal carcinomas , EGF , TGF alpha , cripto , EGFR and c ERBB 2 products were detected at various degrees 24 ( 35 % ) , 50 ( 74 % ) , 31 ( 46 % ) , 11 ( 16 % ) , and 34 ( 50 % ) , respectively . ^^^ A significantly higher incidence ( P < 0 . 05 ) of expression of the following was noted ; TGF alpha in the polypoid carcinomas with an adenoma component , and EGF and c ERBB 2 gene product in the carcinomas without an adenoma component . ^^^ TGF alpha might play a crucial role in carcinomas arising from adenoma , while EGF and c ERBB 2 gene products are strongly indicative of de novo carcinomas . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Epidermal growth factor ( EGF ) and transforming growth factor alpha ( TGF alpha ) stimulate cellular proliferation , whereas epidermal growth factor receptor ( EGFR ) and c erb B 2 oncoprotein are involved in the regulation of cellular growth . ^^^ The present study investigated the prognostic value of these growth factors ( EGF and TGF alpha ) and oncogene products ( EGFR and c erb B 2 ) in TCC UUT . ^^^ Immunohistochemically , EGF was recognized as positive in 50 . 4 % of the samples , TGF alpha in 27 . 8 % , EGFR in 41 . 3 % , and c erb B 2 oncoprotein in 6 . 8 % . ^^^ The immunoreactivity for EGF and c erb B 2 oncoprotein was significantly ( P < 0 . 05 ) correlated with both stage and grade , whereas TGF alpha correlated only with stage and EGFR only with grade . ^^^ Univariate analysis revealed that patients with EGF and c erb B 2 oncoprotein positive tumors had a worse clinical course than patients with EGF and c erb B 2 oncoprotein negative tumors , whereas in the final models of multivariate analysis , stage was found to be the only prognostic factor . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Amplification and overexpression of the c erbB 2 gene in 21MT 2 and 21MT 1 human breast carcinoma cells results in progressively elevated levels of constitutively tyrosine phosphorylated p185erbB 2 and is associated with progressive insulin like growth factor ( IGF ) and combined IGF / epidermal growth factor ( EGF ) independence in culture . ^^^ In addition , the neu differentiation factor / heregulins ( HRGs ) , a family of ligands that activate p185erbB 2 through direct binding to erbB 3 or erbB 4 , are potent mitogens for various nonneoplastic mammary epithelial cells and carcinoma cell lines in the absence of both IGF and EGF in culture . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Whereas ErbB 1 binds to the epidermal growth factor ( EGF ) , both ErbB 3 and ErbB 4 bind to the Neu differentiation factors ( NDFs , or neuregulins ) , and ErbB 2 , the most oncogenic family member , is an orphan receptor whose function is still unknown . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The scFvs were provided with a signal peptide which directs them to the secretory pathway of the cell . scFv 225 , which competes with EGF for binding , functions in an autocrine fashion to inhibit EGF dependent cell growth . scFv FRP 5 was also provided with an endoplasmic reticulum ( ER ) retention signal and inactivates ErbB 2 in an intracrine fashion , by preventing its appearance on the cell surface . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Overexpression of growth factor receptors ( EGF receptor , c erbB 2 , c erbB 3 , TGF beta receptor 1 3 ) , growth factors ( EGF , TGF alpha , TGF beta 1 3 , aFGF , bFGF ) , adhesion molecules ( ICAM 1 , ELAM 1 ) and gene mutations ( p 53 , K ras , DCC , APC ) are present in a significant number of these tumors . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
EGF receptor ( EGF R ) and c erbB 2 are homologous tyrosine kinase transmembrane receptors . ^^^ We studied the location of EGF R and c erbB 2 in 100 samples of normal breast with standard immunohistochemical methods and double labelling techniques . ^^^ EGF R was mainly expressed on the stroma and myoepithelial cells , whereas c erbB 2 expression was exclusively epithelial . ^^^ EGF R and c erbB 2 expression on epithelial cells was stronger during the luteal phase than the follicular phase ( p < 0 . 01 for EGF R ) . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Expression of the type 1 tyrosine kinase growth factor receptors EGF receptor , c erbB 2 and c erbB 3 in bladder cancer . ^^^ Overexpression of the epidermal growth factor ( EGF ) receptor ( 36 per cent , 25 / 70 ) and c erbB 2 proteins ( 9 per cent 6 / 70 ) was determined in the same series of cases . c erbB 3 overexpression was positively correlated with EGF receptor expression ( P < 0 . 025 ) but appeared to be inversely associated with c erbB 2 overexpression . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
At relapse , these cells were shown to overexpress simultaneously at least five oncogenes : HER 2 / neu , ras , EGF receptor , p 53 and c myc . ^^^ Subsequent HER 2 / neu overexpression , EGF receptor overexpression and p 53 protein overexpression were each associated with ras overexpression . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
OBJECTIVE : To test for a significant difference between functional and neoplastic ovarian cyst with respect to epidermal growth factor ( EGF ) receptor and c erbB 2 proto oncogene amplification rates . ^^^ METHODS : We determined amplification of EGF receptor and c erbB 2 genes by differential polymerase chain reaction ( PCR ) on 138 ovarian cyst aspirates . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Induction of EGF receptor and erbB 2 during endotoxin induced alveolar type 2 cell proliferation in the rat lung . ^^^ Specific immunostaining for the proto oncogenes , EGF receptor and erbB 2 , on tissue sections increased during the endotoxin induced hyperplasia . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
These observations are consistent with the proposal that GGF 1 , GGF 2 , and GGF 3 are a set of potent glial cell mitogens and putative ligands of members of the EGF receptor family , namely p 185 neu / erbB2 , p160 / erbB3 , and p180 / erbB4 , which may play important roles in the development , regeneration , and tumor biology of the peripheral nervous system . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
EGF or interleukin 6 stimulation of T47D cells failed to induce CHK SH 2 association with ErbB 2 , the EGF receptor , or the interleukin 6 receptor . ^^^ EGF R , ErbB 3 , and ErbB 4 were not detected in the CHK immunoprecipitates or in the precipitates of the GST SH 2 fusion proteins of CHK , suggesting that the association of CHK with ErbB 2 upon heregulin stimulation is receptor specific ( ErbB 2 ) and ligand specific ( heregulin ) . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The c erbB 2 proto oncogene encodes a 185000 molecular weight protein ( p 185 ( erbB 2 ) ) which shares structural homology with the epidermal growth factor ( EGF ) receptor . ^^^ In contrast , DHT at these levels stimulated EGF receptor protein expression and cellular proliferation . c erbB 2 mRNA levels declined to 30 50 % of control levels following treatment with DHT of 10 ( 10 ) M or greater . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The 180 kDa Shc associated tyrosine phosphoprotein band contains both epidermal growth factor ( EGF ) receptor and p 185 ( neu ) . 3 5 fold increases in EGF receptor but not p 185 ( neu ) tyrosine phosphorylation occur following Gi coupled receptor stimulation . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The EGF family includes EGF , transforming growth factor alpha , heparin binding EGF , amphiregulin , beta cellulin , epiregulin , and heregulins , whereas the receptor family ( the erbB genes ) consists of erbB 1 ( EGF receptor , EGF R ) , erbB 2 , erbB 3 , and erbB 4 . ^^^ The uterine ErbB 2 underwent phosphorylation by several members of the EGF family . ^^^ Collectively , the results place ErbB 2 as a potential candidate receptor subtype for interaction with the EGF related ligands in epithelial cell proliferation / differentiation during the preimplantation period and stromal cell proliferation / decidualization during the postimplantation period . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
In this report we have studied arachidonic acid and linoleic acid metabolism in the human breast carcinoma cell line BT 20 which overexpresses both EGF receptor and the homologous erbB 2 oncogene product . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The epidermal growth factor receptor ( EGF R ) is known to transmodulate the activity and level of the erbB 2 / neu protein in several epithelial cell lines . ^^^ We therefore determined which structural features of the EGF R were important in transmodulating erbB 2 . ^^^ We found that the addition of EGF to nontransformed epithelial cells resulted in down regulation of erbB 2 with the same kinetics and similar extent as the EGF R . ^^^ By using cells expressing a series of EGF R modified by site directed mutagenesis , we found that EGF R tyrosine kinase activity was not necessary for down regulation of erbB 2 , but receptor sequences between 899 and 958 in the EGF R were required . ^^^ To determine whether transmodulation was associated with activation of erbB 2 , tyrosine phosphorylation of erbB 2 was determined following addition of EGF . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Epidermal growth factor receptor ( EGF R ) expression was seen in 86 % of cases and had no prognostic significance . c erbB 2 was expressed in 50 % of cases and was inversely related to a poor prognosis . ^^^ When EGF and TGF alpha were both expressed , there was little or no expression of c erbB 2 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
They also provide an explanation for previously reported dominant negative mutants of the EGF receptor and have interesting implications for the signaling through homo and heterodimers of the family members : EGF receptor , erbB 2 , erbB 3 , and erbB4 . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
In EGFr positive U87MG human glioblastoma cells , expression of the Neu ectodomain inhibits EGF , but not platelet derived growth factor , induced DNA synthesis ; inhibits cell proliferation in the presence of EGF , but not platelet derived growth factor ; inhibits the ability of U87MG to form colonies in soft agar ; and inhibits transforming efficiency in athymic mice . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Three signal transducing transmembrane receptors for neuregulins , which bear significant homology to the EGF receptor , are currently known : HER 2 ( erbB 2 ) , HER 3 ( erbB 3 ) , and HER 4 ( erbB 4 ) . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The following markers were selected on their putative role in the process of metastasis and were studied using immunohistochemical and / or Southern blot techniques : proliferating cell nuclear antigen ( PCNA ) , p 53 , retinoblastoma tumor suppressor gene ( Rb ) , myc , bcl 2 ( inhibitor of apoptosis ) , epidermal growth factor ( EGF ) , EGF receptor ( EGFR ) , neu , nm 23 ( also known as NME 1 , putative metastasis suppressor ) , desmoplakin , neuron cell adhesion molecule ( N CAM ) , epithelial cell adhesion molecule ( Ep CAM ) , E cadherin , cyclin D 1 ( CCND 1 ) , and EMS 1 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Down regulation of cell surface ErbB 1 or ErbB 2 by intracellular expression of specific antibodies has allowed us to delineate the role of these receptors during signaling elicited by : EGF and heparin binding EGF ( HB EGF ) , ligands of ErbB 1 ; betacellulin ( BTC ) , a ligand of ErbB 1 and ErbB 4 ; and neu differentiation factor ( NDF ) , a ligand of ErbB 3 and ErbB 4 . ^^^ Furthermore , EGF and BTC induced activation of ErbB 3 is impaired in the absence of ErbB 2 , suggesting that ErbB 2 has a role in the lateral transmission of signals between other ErbB receptors . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
In cultured mouse keratinocytes , EGF stimulated rapid tyrosine phosphorylation of erbB 2 followed by a time dependent degradation of erbB 2 protein . ^^^ Furthermore , an increase in erbB 2 : EGFr heterodimer formation was also induced by EGF . ^^^ In contrast to the results with erbB 2 , EGF did not induce tyrosine phosphorylation , the degradation of erbB 3 , or erbB 3 : EGFr heterodimer formation in cultured keratinocytes . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
ErbB 2 is an orphan receptor that belongs to a family of tyrosine kinase receptors for either epidermal growth factor ( EGF ) or Neu differentiation factor ( NDF / neuregulin ) . ^^^ One group of tumor inhibitory mAbs ( Class 2 mAbs ) was elicited by the most antigenic site of ErbB 2 , and inhibited in trans binding of NDF and EGF to their direct receptors . ^^^ The inhibitory effect was due to acceleration of ligand dissociation , and it resulted in the reduction of the ability of ErbB 2 to transactivate the mitogenic signals of NDF and EGF . ^^^ ErbB 2 is an orphan receptor that belongs to a family of tyrosine kinase receptors for either epidermal growth factor ( EGF ) or Neu differentiation factor ( NDF / neuregulin ) . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : EGF , transforming growth factor alpha ( TGF alpha ) , EGF receptor ( EGF R ) , and c erb B 2 were determined immunohistochemically in formalin fixed paraffin embedded tumor samples of 30 patients with locally confined RCCs . ^^^ On the contrary , there was a significant correlation between EGF and TGF alpha ( p < 0 . 001 ) , EGF and EGF R ( p = 0 . 028 ) , EGF R and c erb B 2 ( p = 0 . 0009 ) , and inversely related between TGF alpha and tumor stage ( p = 0 . 047 ) and between EGF R and malignancy grade ( p = 0 . 03 ) . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
CEP 751 has no effect on activity of receptors for EGF , IGF 1 , insulin or on erbB 2 ; inhibition of receptors for PDGF and bFGF was observed but occurred with lesser potency than inhibition of trk . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Neu differentiation factor ( NDF ) , a member of the epidermal growth factor ( EGF ) related peptide family , activates ErbB 2 via heterodimerization with the NDF receptors ErbB 3 and ErbB 4 . ^^^ In a similar fashion , EGF receptor ( EGFR ) agonists induce heterodimers of EGFR and ErbB 2 . ^^^ None of the EGFR agonists tested ( EGF , beta cellulin , or heparin binding EGF ) inhibited growth of ErbB 2 overexpressing cells . ^^^ Neu differentiation factor ( NDF ) , a member of the epidermal growth factor ( EGF ) related peptide family , activates ErbB 2 via heterodimerization with the NDF receptors ErbB 3 and ErbB 4 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The co occurrence of Her 2 / neu overexpression and EGF receptor overexpression in the same aneuploid cells defines an adeno / squamous genetic evolutionary sequence that is common to ductal breast cancers , non small cell lung cancers , and other solid tumors . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Here , we report that EGF specifically binds to and can be crosslinked to 32D transfectants co expressing ErbB 2 and ErbB 3 ( 32D . ^^^ E2 / E3 ) , but not to transfectants expressing either ErbB 2 or ErbB 3 individually . [ 125I ] EGF crosslinked species detected in 32D . ^^^ However , EGF and BTC were as effective as HRG in mediating signaling when ErbB 2 was co expressed with the chimera in the 32D . ^^^ Additional data utilizing MDA MB 134 human breast carcinoma cells , which naturally express ErbB 2 and ErbB 3 in the absence of EGFRs , supported the results obtained employing 32D cells and suggest that EGF and BTC may contribute to the progression of carcinomas that co express ErbB 2 and ErbB3 . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
We sought to identify new agents that target and inhibit the activity of the epidermal growth factor ( EGF ) receptor and of c erbB 2 ( also called HER 2 or neu ) , by correlating EGF receptor , transforming growth factor ( TGF ) alpha ( a ligand for EGF receptor ) , and c erbB 2 messenger RNA ( mRNA ) expression levels with the results of cytotoxicity assays of the 49000 compounds in the National Cancer Institute ( NCI ) drug screen database . ^^^ The computer analysis program , COMPARE , was used to search for cytotoxicity patterns in the NCI drug screen database that were highly correlated with EGF receptor , TGF alpha , or c erbB 2 mRNA expression patterns . ^^^ RESULTS : EGF receptor mRNA and TGF alpha mRNA levels were highest in cell lines derived from renal cancers , and c erbB 2 mRNA levels were highest in cells derived from breast , ovarian , and colon cancers . ^^^ Twenty five compounds with high correlation coefficients ( for cytotoxicity and levels of the measured mRNAs ) were tested as inhibitors of the EGF receptor or c erbB 2 signaling pathways ; 14 compounds were identified as inhibitors of these pathways . ^^^ CONCLUSIONS : Novel EGF receptor or c erbB 2 pathway inhibitors can be identified in the NCI drug screen by correlation of cytotoxicity patterns with EGF receptor or c erbB 2 mRNA expression levels . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
PD 158780 reduced clone formation in soft agar of fibroblasts transformed by EGF , EGF receptor , or the neu oncogene but not ras or raf , further demonstrating its high degree of specificity . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The expression of genes involved in mitotic signal pathway ( EGF , TGF alpha , EGF R , erbB 2 , erbB 3 , c myc and c H ras ) was determined immunocytochemically . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
However , EGF could enhance the mRNA expressions of c myc , c erbB 2 and EGFR to various extents , but inhibit that of TGF beta 1 gene . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Reduced ability of transforming growth factor alpha to induce EGF receptor heterodimerization and downregulation suggests a mechanism of oncogenic synergy with ErbB 2 . ^^^ The epidermal growth factor receptor ( EGFR ) is activated by a variety of ligands including EGF and transforming growth factor alpha ( TGFalpha ) , whereas no ligand for the homologous ErbB 2 oncoprotein has yet been identified . ^^^ Here we use both an ErbB 2 phosphoantibody ( aPY 1222 ) and an activation specific EGFR antibody to show that low concentrations of EGF induce more efficient tyrosine phosphorylation of ErbB 2 in A 431 cells than does equimolar TGFalpha , while EGFR is more potently activated by TGFalpha . ^^^ Co precipitation studies confirm that heterodimerization of activated EGFR and transphosphorylated ErbB 2 is readily induced by EGF but not TGFalpha . ^^^ EGFR downregulation is also more efficiently induced by EGF , suggesting that ligand dependent modification of ErbB 2 may be required to terminate EGFR signalling in cells expressing both receptor types . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Neu differentiation factor ( NDF ) is a member of the epidermal growth factor ( EGF ) family of growth factors and is represented by multiple conserved isoforms . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Similarly , EGF treatment of A 431 cells induces concomitant hetero oligomerization of active ErbB 2 with inactive EGFR , of active EGFR with inactive ErbB 2 , and of inactive ErbB 2 with kinase defective ErbB 3 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
In addition to these , only vascular endothelial growth factor , insulin like growth factor 1 and 2 , had significant mitogenic activity , whereas hepatocyte growth factor , nerve growth factor beta , platelet derived growth factors , basic fibroblast growth factor , EGF , transforming growth factor alpha ( TGF alpha ) , neu differentiation factor , and TGF beta were inactive . ^^^ In RINm5F cells a spectrum of erbB gene expression was detected ( EGF receptor / erbB 1 , erbB 2 / neu , and erbB 3 ) , whereas INS 1 cells showed only expression of EGF receptor . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
When singly expressed , neither ErbB 3 nor ErbB 2 can mediate signaling by EGF . ^^^ In addition , when co expressed , blocking either receptor by using site specific antibodies inhibited EGF and betacellulin activities , indicating strict cooperativity between ErbB 3 and ErbB 2 . ^^^ Through analysis of chimeras between EGF and TGFalpha , we identified the middle portion of EGF ( loop B ) as the site that enables activation of ErbB 2 / ErbB 3 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The receptor ( R ) for epidermal growth factor ( EGF ) is expressed at high levels on human breast cancer cells and associates with ErbB 2 , ErbB 3 , and Src proto oncogene family protein tyrosine kinases ( PTKs ) to form membrane associated PTK complexes with pivotal signaling functions . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Members of the type 1 receptor tyrosine kinase family , including epidermal growth factor ( EGF ) receptor ( EGFR ) and ErbB 2 / neu , are often overexpressed in various human cancer cells , including breast . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Despite high oncogene mRNA expression , there was no significant change in the EF 1 delta mRNA level by 5 src , c erbB ( EGF Receptor ) , c erbB 2 , 5 myc and 5 fos oncogenes . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Concentrating on ErbB 1 and two of its ligands , epidermal growth factor ( EGF ) and transforming growth factor alpha ( TGF alpha ) , and the Neu differentiation factor ( NDF / neuregulin ) and one of its receptors , ErbB 3 , we show that ligand binding variably accelerates endocytosis of the respective ligand receptor complex . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
EGF induced redistribution of erbB 2 on breast tumor cells : flow and image cytometric energy transfer measurements . erbB 2 , a member of the epidermal growth factor ( EGF ) receptor type tyrosine kinase receptor family , is overexpressed in breast carcinomas with poor prognosis . ^^^ We found that erbB 2 molecules homoassociate in unstimulated SK BR 3 , BT 474 and BT 474 M ( a metastatic version of the parent BT 474 line ) breast tumor cells , and that the interaction was enhanced by EGF treatment in suspensions of SK BR 3 and BT 474 M cells . ^^^ In accordance with the EGF induced redistribution of erbB 2 , EGF receptor was found to be in close proximity to erbB 2 in FRET measurements . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Co expression of ErbB 2 , a developmentally important co receptor whose expression is frequently elevated in human cancers , specifically potentiated EGF signaling to the level achieved by TGFalpha , an effect that was partially mimicked by ErbB 3 . ^^^ Analysis of the mechanism underlying this trans potentiation implied that EGF driven homodimers of ErbB 1 are destined for intracellular degradation , whereas the corresponding heterodimers with ErbB 2 or with ErbB 3 , dissociate in the early endosome . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
In addition , treatment with EGF results in an enhanced activation of the ErbB 2 receptor in E 5 expressing cells . ^^^ This superactivation must be a result of heterodimer formation between EGFR and ErbB 2 , since EGF is not a ligand for ErbB 2 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
In order to identify whether EGF like growth factors and corresponding receptors ( erbB 2 , erbB 3 , and erbB 4 ) could be involved in the autonomous growth of these transformed rat thyroid epithelial cells , Northern blot for mRNA analysis and Western blot for protein expression were performed . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Structure activity analysis for these compounds and their relative potency and selectivity to inhibit particular RTKs has determined that ( 1 ) 3 [ ( five membered heteroaryl ring ) methylidenyl ] indolin 2 ones are highly specific against the VEGF ( Flk 1 ) RTK activity , ( 2 ) 3 ( substituted benzylidenyl ) indolin 2 ones containing bulky group ( s ) in the phenyl ring at the C 3 position of indolin 2 ones showed high selectivity toward the EGF and Her 2 RTKs , and ( 3 ) the compound containing an extended side chain at the C 3 position of the indolin 2 one ( 16 ) exhibited high potency and selectivity when tested against the PDGF and VEGF ( Flk 1 ) RTKs . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Thus , exogenous EGF induced stage dependent transphosphorylation of ERBB 2 4 as well as ERBB 1 , whereas endogenous phosphorylation of all four receptors peaked in late pregnancy and lactation . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
ErbB 3 was phosphorylated in vivo in response to either heregulin ( HRG ) in cells expressing both ErbB 3 and ErbB 2 , or epidermal growth factor ( EGF ) in cells expressing both ErbB 3 and EGF receptor . ^^^ Separate kinase inactivating mutations in the EGF receptor and ErbB 2 proteins abolished ErbB 3 phosphorylation and signal transduction activated by EGF and HRG respectively . ^^^ Hence the protein tyrosine kinase activity necessary for growth factor signalling via the ErbB 3 protein seems to be provided by coexpressed EGF and ErbB 2 receptor proteins . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
This model system allowed the comparison of EGF activated ErbB 1 with ErbB 1 activated by Neu differentiation factor ( NDF ) induced heterodimerization with ErbB 4 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
This suggests that the mutant EGF R signals through heterodimerization with endogenous , kinase active members of the EGF R family such as ErbB 2 or ErbB 4 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
In contrast , use of a vector in which the extracellular portion of the epidermal growth factor ( EGF ) receptor is fused to the neu tyrosine kinase generates lines of pluripotential nestin positive progenitor cells , which differentiate upon withdrawal of EGF into neurons and glia . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
We show here that following treatment with the progestin , R 5020 , breast cancer cells undergo a `` biochemical shift ' ' in the regulation of epidermal growth factor ( EGF ) stimulated signaling pathways : R 5020 potentiates the effects of EGF by up regulating EGFR , c ErbB 2 and c ErbB 3 receptors , and by enhancing EGF stimulated tyrosine phosphorylation of signaling molecules known to associate with activated type 1 receptors . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
In TNF sensitive breast and cervical carcinoma cells activation of EGFR or ErbB 2 by the exogenous growth factors EGF and heregulin beta 1 resulted in a significant increase in the number of cells surviving TNF alpha treatment . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The human epidermal growth factor receptor ( HER or ErbB ) family consists of four distinct members , including the epidermal growth factor ( EGF ) receptor ( EGFR , HER 1 , or ErbB 1 ) , ErbB 2 ( HER 2 or neu ) , ErbB 3 ( HER 3 ) , and ErbB 4 ( HER 4 ) . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
We previously described spatiotemporal expression of various epidermal growth factor ( EGF ) like ligands and receptor subtypes , ErbB 1 and ErbB 2 , during the peri implantation period . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Co expression of full length EGFR and oncogenic p 185 ( neu ) receptors resulted in an increased EGF induced phosphotyrosine content of p 185 ( neu ) , increased cell proliferation to limiting concentrations of EGF , and increases in both EGF induced MAPK and phosphatidylinositol 3 kinase ( PI 3 kinase ) activation . ^^^ Intracellular domain deleted p 185 ( neu ) receptors ( T691stop neu ) were able to associate with full length EGFR , but induced antagonistic effects on EGF dependent EGF receptor down regulation , cell proliferation , and activation of MAPK and PI 3 kinase pathways . ^^^ Ectodomain deleted p 185 ( neu ) proteins ( TDelta 5 ) were unable to physically associate with EGFR , and extracellular domain deleted p 185 ( neu ) forms failed to augment activation of MAPK and PI 3 kinase in response to EGF . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
By immunoprecipitation followed by Western blotting we showed that ErbB1 / ErbB3 heterodimers are the major mitogenic signaling entity in 1 , 25 ( OH ) 2D3 stimulated cells . 1 , 25 ( OH ) 2D3 did not affect the levels of the proteoglycan dependent EGFR ligands amphiregulin and heparin binding EGF nor the synthesis of proteoglycans , as assessed by 35S labeling and ion exchange chromatography . 1 , 25 ( OH ) 2D3 caused a marked increase in the cellular contents of ErbB 1 , ErbB 2 , and ErbB 3 proteins . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Augmentation of a humanized anti HER 2 mAb 4D5 induced growth inhibition by a human mouse chimeric anti EGF receptor mAb C 225 . ^^^ Overexpression of epidermal growth factor ( EGF ) receptor and HER 2 ( p185neu ) may both contribute to the growth of human cancers . ^^^ A humanized anti HER 2 monoclonal antibody ( mAb ) 4D5 and a human mouse chimeric anti EGF receptor mAb C 225 are currently being investigated in clinical trials for their anti tumor activities . ^^^ Our data suggest the potential fruitful cooperation of anti EGF receptor mAb and anti HER 2 mAb in the treatment of human cancers stimulated by EGF receptor and HER 2 signals . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
CONCLUSION : It is suggested that the aberration of c erbB 2 , p21ras , EGF and EGFR might be the early events in the initiation and progression of gliomas , whereas p 53 is involved in all stages of these tumors . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Here we use isoform specific EGFR and ErbB 2 antibodies to show that the duration of EGFR signalling induced by a single TGFalpha exposure is less than that induced by equimolar EGF . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
In contrast , EGFR truncation did not impair EGF mitogenic signaling , and in c ' 1000 cells EGF was able to stimulate the association of ErbB 2 with GRB 2 and SHC . ^^^ Conversely , overexpression of ErbB 2 ( 1 813 ) inhibited EGF stimulated Elk 1 transactivation in c ' 1000 but not in WT cells . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
In addition to the cell adhesion function , beta catenin , which is an intracytoplasmic cadherin binding molecule and thought to be a regulator of cadherin mediated cell adhesion function , has been proven to associate with both the growth factor receptors , including c erbB 2 , EGF receptor and k sam and APC tumor suppressor gene product . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
TGF alpha stimulated tyrosine phosphorylation of both the EGF receptor and c erbB 2 receptor in all four cell lines . ^^^ The inhibitor ZM 252868 , at concentrations > or =0 . 3 microM , completely inhibited TGF alpha stimulated tyrosine phosphorylation of the EGF receptor and reduced phosphorylation of the c erbB 2 protein . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Here , we contrasted these two models of ErbB 2 function : First , examination of a large series of epidermal growth factor ( EGF ) like ligands and neuregulins , including virus encoded ligands as well as related motifs derived from the precursor of EGF , failed to detect interactions with ErbB 2 when this protein was singly expressed . ^^^ Second , by using antibodies that block inter ErbB interactions and cells devoid of surface ErbB 2 , we learned that signaling by all ligands examined , except those derived from the precursor of EGF , was enhanced by the oncoprotein . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Tumor markers ( CA 125 , cancer associated serum antigen ( CASA ) , CA 72 4 ) , hormones ( estradiol ( E 2 ) , follicle stimulating hormone ( FSH ) , luteinizing hormone ( LH ) ) , epidermal growth factor ( EGF ) receptor and c erb B 2 amplification rate were detected in cyst fluid . ^^^ There were no significant correlations between EGF receptor ( P = 0 . 14 ) and c erb B 2 ( P = 0 . 06 ) gene amplification rates and malignant histology . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Endocytosis deficiency of epidermal growth factor ( EGF ) receptor ErbB 2 heterodimers in response to EGF stimulation . ^^^ Epidermal growth factor ( EGF ) stimulates the homodimerization of EGF receptor ( EGFR ) and the heterodimerization of EGFR and ErbB 2 . ^^^ ErbB 2 was not endocytosed in response to EGF stimulation . ^^^ Moreover , in MDA 453 , SKBR 3 , and BT 474 cells , which have very high levels of EGFR ErbB 2 heterodimerization , EGF induced EGFR endocytosis was greatly inhibited compared with that in BT 20 cells , which have a very low level of EGFR ErbB 2 heterodimerization . ^^^ Microinjection of an ErbB 2 expression plasmid into BT 20 cells significantly inhibited EGF stimulated EGFR endocytosis . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
ErbB 2 ( HER 2 , Neu ) , a member of the epidermal growth factor ( EGF ) receptor tyrosine kinase family , is often overexpressed in breast cancer and other malignancies . ^^^ ErbB 2 homodimerizes but also presents as a common auxiliary subunit of the EGF and heregulin receptors ( erbB 1 or EGFR ; and erbB 3 4 , respectively ) , with which it heteroassociates . ^^^ Activation of SKBR 3 cells with EGF , heregulin as well as a partially agonistic anti erbB 2 monoclonal antibody led to an increase in the mean cluster diameter to 0 . 6 0 . 9 microm , irrespective of the ligand . ^^^ The EGF induced increase in the erbB 2 cluster size was inhibited by the EGFR specific tyrosine kinase inhibitor PD 153035 . ^^^ ErbB 2 ( HER 2 , Neu ) , a member of the epidermal growth factor ( EGF ) receptor tyrosine kinase family , is often overexpressed in breast cancer and other malignancies . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The panel of potential SEBs of malignancy include p 53 , cytomorphometric indices , ploidy , PNCA , erbB 2 , erbB 3 , EGF receptor , TGF alpha tumor associated glycoprotein 72 , fatty acid synthetase and Lewis Y antigen . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
While Western blot analysis initially indicated that GI 101A was ER , analysis of ER mRNA by RT PCR and RPA have demonstrated the expression of ER ( as well as EGF receptor and neu oncogene ) transcripts . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
In this report we show that ErbB 2 , ErbB 3 , and ErbB 4 do not potentiate Stat 5 phosphorylation by EGF . ^^^ However , we provide evidence that EGF and neu differentiation factor induced Stat activation are dependent on Src but not Jak kinases . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Here we show that PKC dependent transmodulation of EGFR and ErbB 2 signalling is schedule specific : prolonged pre treatment of A 431 cells with the PKC agonist phorbol dibutyrate potently inhibits subsequent ligand induced EGFR signalling as expected , but EGF pre treatment reverses the inhibitory effect of phorbol . ^^^ Because these findings suggested a synergistic interaction between tyrosine and PKC dependent phosphorylation events , we sought to define the interactions of tyrosine phosphorylated and PKC modified ErbB 2 subsets within EGF inducible hetero oligomers . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
EGF treatment of EGFR transfectants results in more rapid EGFR downregulation than occurs in ErbB 2 transfectants , but Northern blot analysis demonstrates reduced basal EGFR gene expression in ErbB 2 transfectants . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Epidermal growth factor ( EGF ) increased the HMG CoA reductase activity , protein , and mRNA levels only in ErbB 2 expressing cells ( SKBR 3 and MCF 7 ) but not in MDA MB 468 cells that do not express ErbB 2 even though their EGF receptor was efficiently phosphorylated . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The heart of the minipigs was also enlarged , an interesting finding regarding interactions between the different parts of the EGF system , as knock out mice of the receptors erbB 2 and erbB 4 die due to maldevelopments in the heart . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
They ( a ) increase astrocytic gene expression of at least one of the EGF related ligands ( TGFalpha ) , ( b ) increase expression of at least two of the receptors ( erbB 4 and erbB 2 ) , and ( c ) enhance the LHRH response to PGE ( 2 ) by up regulating in LHRH neurons the expression of specific PGE ( 2 ) receptor isoforms . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Inhibition of pp60c Src reduces Bcl XL expression and reverses the transformed phenotype of cells overexpressing EGF and HER 2 receptors . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
In addition , EGF receptor , c erbB 2 and c erbB 3 levels were reduced , and the basal and EGF stimulated mitogen activated protein kinase activities were reduced . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Involvement of the c erbB 2 oncogene product in the EGF induced cell motility of SK OV 3 ovarian cancer cells . ^^^ These data indicate that the c erbB 2 oncogene product is essential to mediate the motility signal of EGF to SK OV 3 ovarian cancer cells . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
We have reported that the transformation of human lung epithelial cells by c erbB 2 also requires an active ErbB 1 ( EGF receptor ) and the autocrine production of its ligand , TGF alpha . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The ErbB 2 tyrosine kinase functions as coreceptor for the neuregulin receptors ErbB 3 and ErbB 4 and can participate in signaling of EGF receptor ( ErbB 1 ) , interleukin receptor gp 130 , and G protein coupled receptors . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
We analyzed the formation of homo and heterodimers between EGFR , ErbB 2 , and ErbB 3 ( members of the EGF receptor family ) in the human skin keratinocyte cell line HaCaT , in dependence of the added ligand . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Consistent with the notion that transforming erbB complexes induce sustained and unregulated MAPK activities , coexpression of p 185 ( neu ) and EGFR proteins to levels sufficient to transform murine fibroblasts also resulted in prolonged EGF induced ERK in vitro kinase activation . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Further studies revealed that , while both EGF ( 100 ng / ml ) and CCh ( 100 microM ) stimulated phosphorylation of the EGFR , only EGF stimulated phosphorylation of ErbB 2 , and neither stimulated ErbB 3 phosphorylation . ^^^ EGF , but not CCh , stimulated the formation of EGFR / ErbB2 receptor dimers and the recruitment of p 85 to ErbB 2 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The primary cell surface targets of heregulins are heterodimers of the EGF receptor homolog HER 2 with either HER 3 or HER 4 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Ligand dependent activation of HER 1 , HER 3 , and HER 4 by EGF or heregulin results in heterodimerization and , thereby , HER 2 activation . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Its IC 50 values were 0 . 1 microgram / ml ( 0 . 38 microM ) for EGF receptor kinase , 3 micrograms / ml ( 11 microM ) for ErbB 2 , and 100 micrograms / ml ( 380 microM ) or more than that for other kinases including PDGF receptor , 5 src and protein kinase C . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Cells stimulated with EGF related peptides show increased invasion of the extracellular matrix , whereas cells devoid of functional ErbB 2 receptors do not . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Expression of the EGF receptor family members ErbB 2 , ErbB 3 , and ErbB 4 in germinal zones of the developing brain and in neurosphere cultures containing CNS stem cells . ^^^ The epidermal growth factor receptor family consists of four related tyrosine kinases : the epidermal growth factor receptor ( EGF R or ErbB ) , ErbB 2 , ErbB 3 , and ErbB 4 . ^^^ Since EGF R is expressed by proliferating cells in the central nervous system ( CNS ) , including multipotent CNS stem cells , we examined the expression of ErbB 2 , ErbB 3 and ErbB 4 in the germinal epithelia of the developing rat brain using in situ hybridization . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Immobilized proteolytic enzyme cartridges were used to rapidly digest neu differentiation factor EGF domain in order to obtain improved peptide maps useful for assignment of disulfide linkages . ^^^ Using these immobilized columns , we demonstrated that disulfide structure assignment of the EGF domains of recombinant human neu differentiation factor can be performed by isolation of individual disulfide containing peptides followed by assignment of disulfide linkages with prompt fragmentation of peptides using matrix assisted laser desorption / ionization time of flight mass spectrometry . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
On the phenotypic level , increased expression and changes in pattern of expression of proliferation marker ( Mib 1 ) Ki 67 antigen , overexpression of p 53 protein , overexpression of growth factors such as epidermal growth factor ( EGF ) , c erbB 2 , and transforming growth factor ( TGF ) a , decreased and abnormal expression of the cell adhesion molecule E cadherin , and , in carcinomas , increased expression of serine proteases have all been described . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
A series of monoclonal antibodies ( MAbs ) 3 directed against the EGF ( ErbB 1 ) receptor and the closely related HER2 / Neu ( ErbB 2 ) receptor are currently under evaluation . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The oncogene erbB 2 ( c neu ) and the growth factor EGF expression were more prominent in the small tumors of the mice treated with arotinoid than in the larger tumors of the control group . ^^^ PCNA staining was observed in areas where there was high erbB 2 and EGF staining . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Consistently the predominant interaction after EGF treatment was between EGFR and HER 2 , whereas activation of HER 3 and HER 4 depended on the relative abundance of the four receptors in the cells . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Recombinant antibody toxins specific for ErbB 2 and EGF receptor inhibit the in vitro growth of human head and neck cancer cells and cause rapid tumor regression in vivo . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Cell lines stably expressing HER 2 and the receptors for hepatocyte growth factor ( HGF ) , vascular endothelial growth factor ( VEGF ) , insulin like growth factor 1 ( IGF 1 ) and epidermal growth factor ( EGF ) have been established . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Expression of mRNA for the ligands EGF and betacellulin and of the receptors HER 1 and HER 2 were similar in all lines investigated , except LNCaP cells which exhibit low expression of HER 1 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Basal growth of MCF 7 cells was unaffected by co administration of the growth factors EGF , TGF alpha , IGF 1 , and IGF 2 , and the new agents did not inhibit EGFR and c erbB 2 autophosphorylation in cell lysates from MDA 468 or SkBr 3 cells , respectively , suggesting that receptor tyrosine kinases are not targets for these compounds . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The ErbB receptor tyrosine kinase family consists of the epidermal growth factor ( EGF ) receptor ( ErbB 1 ) and three related receptors ( ErbB 2 , ErbB 3 , ErbB 4 ) . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
To assess whether ErbB 2 is a critical component in epidermal growth factor ( EGF ) mediated stimulation of tumor cell proliferation , we used as a model SK OV 3 ovarian cancer cells , which over express EGF receptor ( EGFR ) and ErbB 2 receptors . ^^^ The induction of colony formation by EGF was completely abrogated in ErbB 2 depleted cells , despite unchanged expression levels and tyrosine phosphorylation of the EGFR . ^^^ The duration of EGF mediated c Fos mRNA up regulation was decreased in parallel with loss of ErbB 2 expression . ^^^ Our results demonstrate that in human ovarian cancer cells the EGFR ErbB 2 heterodimer , and not the EGFR homodimer , can be rate limiting for EGF mediated proliferation , thus suggesting that the oncogenic activity of ErbB 2 in human tumors is due in part to its ability to increase the growth response to stroma derived EGF like growth factors . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The nontransformed H16N 2 cells isolated from normal tissue express EGFR , p 185 ( erbB 2 ) , and erbB 3 , and are highly responsive to the mitogenic effects of HRG beta as well as to the combination of EGF and insulin in serum free culture . ^^^ Furthermore , erbB 3 principally mediated the direct recruitment of p 85 in cells stimulated by HRG or EGF , indicating that , in addition to the high level activation of PI3K by p 185 ( erbB 2 ) / erbB 3 , EGFR / erbB 3 heterodimer interaction is essential for the weak but significant level of PI3K activated by EGF in cells that express normal EGFR levels . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Geldanamycin ( GA ) and herbimycin ( HA ) , specific inhibitors of the cytosolic chaperone HSP 90 and its endoplasmic reticulum homologue GRP 94 , were shown to accelerate degradation of the EGF R and of its homologue p 185 ( c ) ( erbB 2 ) . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Evidence is provided for trophoblast cell expression of epidermal growth factor receptor ( EGFR ) , ErbB 2 , fibroblast growth factor receptor 1 ( FGFR 1 ) , transforming growth factor alpha , and heparin binding EGF . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The HER 2 / erbB 2 / c neu proto oncogene encodes for an EGF receptor like protein which has been implicated in the pathogenesis of several human malignancies . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
We have developed a method for the quantitation of mRNA coding for all six growth factors activating the human EGF receptor ( HER 1 ) and for the quantitation of mRNA for the receptors HER 1 and its preferred dimerization partner , HER 2 . ^^^ The developed method has enabled us to demonstrate that prostate stromal cells in primary culture express EGF , heparin binding EGF ( HB EGF ) , amphiregulin , betacellulin , and epiregulin as well as the HER 1 and HER 2 receptors , whereas no transforming growth factor alpha mRNA is found . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Ras was highly activated compared to benign tissue in 11 of the 20 cancers ; 7 of these 11 cancers expressed both the epidermal growth factor ( EGF ) and ErbB 2 / neu / HER 2 receptors with the remaining four cancers with high Ras activation expressing one of these two receptors . ^^^ In the other nine cancers , Ras activation was similar to that observed in benign breast tissue with none of these cancers expressing the EGF receptor while one expressed the ErbB 2 receptor . ^^^ Thus , Ras was abnormally activated in breast cancers overexpressing the EGF and / or ErbB 2 receptors indicating there are sufficient ligands in vivo to activate these receptors , and this work provides a basis for new target based treatments of this disease . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Estrogen and progesterone facilitate erbB mediated glia to LHRH neuron communication by enhancing astrocytic gene expression of at least one of the EGF related ligands ( TGFalpha ) and two of the receptors ( erbB 2 and erbB 4 ) . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
This pathway involves at least two distinct receptor kinases : EGF receptor ( ErbB 1 ) and ErbB 2 . ^^^ For this purpose , we used PP 1 , an inhibitor specific for Src family kinases , which does not inhibit either EGF receptor or ErbB 2 . ^^^ CONCLUSION : Like EGF receptor and ErbB 2 , a member of Src family kinases ( most likely a new Src related kinase called `` Ray ' ' ) is essential for the Ras induced activation of PAK and the malignant transformation both in vitro and in vivo . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Enhanced drug resistance in cells coexpressing ErbB 2 with EGF receptor or ErbB 3 . ^^^ To further determine the role of the ErbB receptors in drug resistance , we selected various NIH 3T3 cell lines that specifically expressed EGF receptor ( EGFR ) , ErbB 2 , ErbB 3 , EGFR / ErbB2 , EGFR / ErbB3 , or ErbB2 / ErbB3 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Growth factor mediated EF 1alpha expression was effectively blocked by pretreatment with humanized anti EGF receptor antibody C 225 or anti human epidermal growth factor receptor 2 ( HER 2 ) antibody herceptin . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
RESULTS : Messenger RNA encoding the EGF receptors ( EGFR ) c erbB 2 and c erbB 3 , but not c erbB 4 , was detected in primary cultures of human nasal epithelial cells . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
This growth factor mediated hnRNP K expression was effectively blocked by pretreatment of cultures with humanized anti EGF receptor ( EGFR ) antibody C 225 , or anti human epidermal growth factor receptor 2 ( HER 2 ) antibody . ^^^ These results suggested that the activity of human EGF receptor family members regulates hnRNP K expression by extracellular growth promoting signals and that therapeutic humanized antibodies against EGFR and HER 2 can effectively block this function . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
CONCLUSION : Aberrations of c erbB 2 , p21ras , EGF and EGF receptor might be early events in the initiation and progression of astrocytomas , whereas p 53 overexpression is involved in all the stages . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Down regulation of ErbB receptor activity , through the use of specific pharmacological inhibitors or dominant negative receptor constructs , revealed that IL 6 induced MAPK activation was largely dependent on epidermal growth factor ( EGF ) receptor activity , but not on ErbB 2 activity . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The ErbB family of receptors , which includes the epidermal growth factor receptor ( EGFR ) , ErbB 2 , ErbB 3 , and ErbB 4 , mediate signaling by EGF like polypeptides . ^^^ K721M alone exhibited no detectable signaling capacity , whereas coexpression of K721M with ErbB 2 , but not ErbB 3 or ErbB 4 , resulted in EGF dependent mitogen activated protein kinase ( MAPK ) activation . ^^^ The kinase activity , but not tyrosine phosphorylation , of ErbB 2 was required for EGF induced MAPK activation . ^^^ EGF was found to activate the pro survival protein kinase Akt in stable cell lines expressing K721M and ErbB 2 but , unlike cells expressing wild type EGFR , was incapable of activating signal transducers and activators of transcription ( STAT ) or driving cell proliferation . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Treatment of cells with EGF ( ErbB 1 specific ) or heregulin ( ErbB 4 specific ) resulted in a hierarchic transactivations of ErbB 2 and ErbB 3 dependent on GF binding specificity . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Heterodimerization of the epidermal growth factor ( EGF ) receptor and ErbB 2 and the affinity of EGF binding are regulated by different mechanisms . ^^^ Furthermore , the EGF induced tyrosine phosphorylation of ErbB 2 was not affected by overexpression of [ K44A ] dynamin , implying that EGFR ErbB 2 dimers were fully functional . ^^^ Our results strongly suggest that high affinity binding of EGF and EGFR ErbB 2 heterodimerization are regulated by different mechanisms . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
At the site of implantation , uterine luminal epithelial cells apposing the preimplantation blastocyst displayed a distinct membrane immunolocalization of ErbB 2 , identifying the uterine epithelium as target for EGF , TGFalpha , and HB EGF derived from both the embryonic trophectoderm and the uterine epithelium . ^^^ The detection of tyrosine phosphorylated ErbB 2 in the rabbit placenta indicated the presence of a functional ErbB / EGF like system in the pregnant rabbit uterus . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The HER2 / neu gene product , a transmembrane protein kinase member of the EGF receptor family , has significant potential as a tumor antigen for vaccination . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Human carcinomas frequently express high levels of receptors in the EGF receptor family , and overexpression of at least two of these receptors , the EGF receptor ( EGFr ) and closely related ErbB 2 , has been associated with a more aggressive clinical behavior . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Expression of individual EGF receptor ( EGFR ) and ErbB 2 , ErbB 3 , or ErbB 4 receptors was detected in 72 . 2 , 44 . 5 , 56 . 3 , and 29 . 8 % of bladder cancer cases , respectively . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The expression of oncogenes ( ras , erbB 2 and EGF receptor ) , tumor suppressor genes ( Rb , p 53 ) , cell cycle genes ( p 15 , p 16 ) and DNA repair genes is altered mostly by mutation or chromosomal aberration . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Our results indicate that while there are measurable differences in the abilities of the two mutant receptors to stimulate [ ( 3 ) H ] thymidine incorporation between 20 and 24 h after addition of EGF , these differences can not be correlated with significant differences in EGF stimulated tyrosine phosphorylation of mutant EGF receptor and endogenous ErbB 2 , the extent of receptor internalization , EGF stimulated ion uptake , stimulation of SHC activity , or receptor association with Grb 2 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
RESULTS : After stimulation with EGF BT 474 , but not SK BR 3 , cells formed EGFR / c erbB 2 receptor complexes . ^^^ In contrast , the concurrent activation of MAPK and PKB / Akt by EGF treatment led to an inhibition of proliferation in SK BR 3 and can be attributed to missing EGFR / c erbB 2 heterodimers . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The mRNA content for six ligands [ EGF , transforming growth factor alpha ( TGF alpha ) , amphiregulin ( AR ) , betacellulin ( betaCL ) , heparin binding EGF like growth factor ( HB EGF ) , epiregulin ( EPI ) ] and two receptors [ EGF receptor 1 Human EGF Receptor ( HER 1 ) and 2 ( HER 2 ) ] was examined by a newly developed quantitative reverse transcription PCR method . ^^^ Five ligands and two receptors ( HER 1 and HER 2 ) were present in median concentrations of ( 10 ( 21 ) mol / microg RNA ) 0 . 39 ( AR ) , 11 ( betaCL ) , 2 . 4 ( EPI ) , 40 ( HB EGF ) , 1 . 4 ( TGF alpha ) , 75 ( HER 1 ) , and 39 , 000 ( HER 2 ) . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The expression of epidermal growth factor ( EGF ) receptor ( P = 0 . 037 ) , transforming growth factor alpha ( TGF alpha ; P < 0 . 001 ) , and HER 2 ( P = 0 . 066 ; positive trend ) correlated with activation of ERK1 / 2 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Expression of herstatin , an autoinhibitor of HER 2 / neu , inhibits transactivation of HER 3 by HER 2 and blocks EGF activation of the EGF receptor . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Six out of 11 human pancreatic cancer cell lines expressed all four epidermal growth factor ( EGF ) receptor family members ( HER 1 ( EGF R ) , HER 2 , HER 3 , and HER 4 ) , including Panc 89 cells . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Tumor bearing mice were used to compare the in vivo efficacy of two anti cancer agents : PKI 166 , a kinase inhibitor selective for EGF receptor and ErbB 2 , and Taxol , a microtubule assembly blocker . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
ErbB 2 is a receptor tyrosine kinase belonging to the family of epidermal growth factor ( EGF ) receptors which is generally involved in cell differentiation , proliferation , and tumor growth , and activated by heterodimerization with the other members of the family . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The expression of epidermal growth factor ( EGF ) , epidermal growth factor receptor ( EGFR ) , transforming growth factor alpha ( TGF alpha ) , and of c erbB 2 was immunohistochemically investigated in resected gastric carcinoma ( in 39 cases of superspreading type and in 11 cases of penetrating type ) , to understand the differential biological features of these two types of gastric carcinoma . ^^^ EGF , EGFR and c erbB 2 positive cases were preferentially found in penetrating type rather than in superspreading type ( p < 0 . 05 , p < 0 . 01 , and p < 0 . 05 , respectively ) . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Activation of ErbB1 / EGFR is mainly triggered by epidermal growth factor ( EGF ) and other related ligands , while activation of ErbB 2 , ErbB 3 , and ErbB 4 receptors occurs by binding to another set of EGF like ligands termed neuregulins ( NRGs ) . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
It also inhibited proliferation in EGFR ( + ) cancer cell lines overexpressing erbB 2 ( SKBr 3 , SKOV 3 , BT 474 ) by between 20 % and 80 % , effects which correlated with inhibition of EGF dependent erbB 2 phosphorylation and activation of ERK MAP kinase and PKB / Akt in SKOV 3 cells . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The authors ' results may partially explain why OVCA patients with positive macrophage colony stimulating factor ( a chemoattractant of IL 1alpha secreting monocytes ) or EGF receptors ( c erb B 2 ) have a poor prognosis . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
We have extended the use of stopped flow mixing and fluorescence anisotropy detection to investigate in real time the effects of ErbB 2 coexpression on the kinetic interactions of epidermal growth factor ( EGF ) with the EGF receptor . ^^^ Using stable 32D derived cell lines expressing both the EGF receptor and ErbB 2 , and fluorescein labeled H22Y murine EGF ( F EGF ) , a series of association and dissociation experiments were performed in which the kinetic interaction of F EGF with cells was monitored by observing time dependent changes in fluorescence anisotropy following rapid mixing . ^^^ Data were collected at various concentrations of F EGF and multiple cell densities , using cells that express similar levels of the EGF receptor but different levels of ErbB 2 , and then analyzed by fitting to a two independent receptor class model using global analysis techniques . ^^^ Further , the increase in the fraction of high affinity receptors due to the presence of ErbB 2 occurred without any change in the total number of EGF binding sites per cell . ^^^ Thus , we have identified modulation of the relative populations of high and low affinity classes of EGF receptors as a consequence of coexpression of ErbB 2 with the EGF receptor . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Pro oncogenic factors that also release beta catenin from the adherens complex and / or encourage translocation to the nucleus include ras , epidermal growth factor ( EGF ) , c erbB 2 , PKC betaII , MUC 1 , and PPAR gamma , whereas anti oncogenic factors that also inhibit nuclear beta catenin signaling include transforming growth factor ( TGF ) beta , retinoic acid , and vitamin D . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
PURPOSE : This study was conducted to evaluate the clinical significance of the localization of epidermal growth factor receptor ( EGF r ) , transforming growth factor ( TGF ) alpha , and erbB 2 in the development , progression and prognosis of squamous cell cancers ( SCCs ) of the lung . ^^^ EXPERIMENTAL DESIGN : The localization of EGF r , TGF alpha , and erbB 2 was evaluated immunohistochemically in 60 archival specimens of SCC of the lung and in 60 lung specimens without cancer . ^^^ RESULTS AND CONCLUSIONS : The expression of EGF r , erbB 2 , and TGF alpha were significantly higher in SCC and associated precancerous lesions than in the normal bronchial epithelium and hyperplastic lesions of noncancer specimens . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
ErbB 2 , a member of the epidermal growth factor ( EGF ) receptor tyrosine kinase family , is often overexpressed and / or amplified in breast , ovarian and gastric cancers , and other malignancies . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
RESULTS : Prostate tissue expressed the EGF receptor ( HER 1 ) and HER 2 , and the ligands EGF , transforming growth factor alpha ( TGFalpha ) , heparin binding ( HB ) EGF , betacellulin and amphiregulin . ^^^ Treatment with finasteride produced greater concentrations of amphiregulin ( P < 0 . 05 ) than did placebo , did not change the level of TGFalpha , HER 1 and HER 2 , and tended to decrease the concentration of EGF , betacellulin and HB EGF in prostate tissue . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Activation mutations in the K ras oncogene occur in around 90 % of pancreatic cancers , and the overexpression of growth factors epidermal growth factor ( EGF ) , transforming growth factor ( TGF ) alpha , TGFbetas 1 3 , acidic fibroblast growth factor ( aFGF ) , basic FGF ( bFGF ) , and growth factor receptors c erbB 2 and 3 and TGFRbetas 1 3 is common . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
AR induced a slight induction of ErbB 2 on duct cells , whereas TGF alpha resulted in overexpression of the EGF receptor in cells within tubular complexes . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
In A 549 and H 322 cells , which express a detectable amount of EGF R ( ErbB 1 ) , ErbB 2 , ErbB 3 , and ErbB 4 receptors , the LA 1 mAb induces up regulation of the E cadherin / catenin complex ( alpha , beta , and gamma catenins ) . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
A cluster corresponding to normal , differentiated kidney cells included ERBB 2 ( HER 2 ) for receptor protein tyrosine kinase , several phosphatase genes ( PTPRE , PTPRB , DUSP 9 ) , and EGF . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
A2 tumor cells with HER 2 receptor agonists ( EGF and NDF ) enhanced tumor ability to activate CTL from tumor associated lymphocytes ( TAL ) and from T cells from peripheral blood in vitro . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Here we report that the epidermal growth factor ( EGF ) induced cell migration of breast cancer cells is attributed to a transient , rather than a sustained , activation of phospholipase C ( PLC ) gamma 1 due to c erbB 2 signaling . ^^^ In contrast , c erbB 2 / EGFR expressing cells displayed baseline transient calcium oscillations after EGF treatment due to short term PLC gamma 1 tyrosine phosphorylation and short term IP 3 and DAG turnover . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Crosslinking also decreased ErbB 2 ErbB3 heteroassociation and the EGF and heregulin induced tyrosine phosphorylation of Shc . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The effects of the treatment on mRNA and protein levels of human epidermal growth factor ( EGF ) receptors ( EGFR and HER 2 ) in ovarian tumors were determined by RT PCR and immunoblotting . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The neuregulin ( NRG ) / epidermal growth factor ( EGF ) family of growth factors consists of several ligands that specifically activate four erbB receptor tyrosine kinases , namely erbB 1 ( EGF R ) , erbB 2 ( neu ) , erbB 3 , and erbB 4 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The Her 2 / neu oncogene , the second member of the epidermal growth factor ( EGF ) receptor family , encodes a transmembrane tyrosine kinase receptor . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Transfection of MCF 10A with individual erbB 2 containing episomes induced significant foci formation in low serum ( 0 . 1 % ) without EGF . ^^^ These results show that if wild type erbB 2 is sufficiently overexpressed , erbB 2 alone can cause EGF independent transformation of these nonmalignant breast cells . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The use of various pharmacological inhibitors indicated that gastrin stimulated migration via activation of the EGF receptor ( EGR R ) , the erbB 2 receptor tyrosine kinase , and the MAP kinase pathway . ^^^ Flow cytometry indicated that in both cell types , gastrin increased MAP kinase via activation of EGF R but not FGF R 1 or erbB 2 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
We have established a series of stable , monoclonal 32D derived cell lines that express the epidermal growth factor ( EGF ) receptor , ErbB 2 , or a combination of both at different ratios . ^^^ Increasing the relative expression of ErbB 2 leads to an increase in the fraction of high affinity class receptors observed , without altering the total number of EGF binding sites . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
DN erbB 4 expression is most abundant in hypothalamic astrocytes , where it blocks the ligand dependent activation of glial erbB 4 and erbB 2 receptors , without affecting erbB 1 ( EGF ) receptor signaling . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The cardiac valve and the ventricular chamber phenotypes resembled those displayed by mice lacking EGFR , a receptor for HB EGF , and by mice conditionally lacking ErbB 2 , respectively . ^^^ HB EGF induced tyrosine phosphorylation of ErbB 2 and ErbB 4 , and to a lesser degree , of EGFR in cardiac myocytes . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
For ErbB 2 , GH induces serine / threonine phosphorylation that dampens basal and EGF induced ErbB 2 kinase activation . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Therapeutic antibodies directed against tumor necrosis factor alpha ( TNF alpha ) for the treatment of rheumatoid arthritis , and against the human EGF receptor 2 ( HER 2 ) receptor for the treatment of breast cancer have provided significant clinical benefit for the patients . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
While HER 1 ( EGF receptor ) and HER 2 have been extensively studied , little is known about the clinical significance of HER 3 and especially HER 4 expression . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
RT PCR analysis revealed that the EGF receptors erbB 1 , erbB 2 , erbB 3 and erbB 4 were expressed at all stages of the spermatogenic wave , whereas differential expression was found in isolated Leydig , Sertoli and peritubular cells . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The EGF Receptor ( EGFR ) , the first transmembrane receptor tyrosine kinase cloned and sequenced , and its closely related family members HER 2 , HER 3 , and HER 4 , play myriad roles in mammalian growth and development . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Among the best studied growth factor receptors are the two members of EGF receptor familiy EGFr ( ErbB 1 ) , and Her2 / neu ( ErbB 2 ) . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Coimmunoprecipitation experiments also indicated that Grb 7 and NIK could be simultaneously recruited into signaling complexes of all known EGF / heregulin receptors , including EGFR , ErbB 2 , ErbB 3 , and ErbB 4 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Furthermore , inhibiting EGF receptor signaling using antibodies against ErbB 2 ( Herceptin ) resulted in increased hypoxia induced cell death in MCF 7 cells . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Inhibition of ErbB 2 and ErbB 3 expression by quercetin prevents transforming growth factor alpha ( TGF alpha ) and epidermal growth factor ( EGF ) induced human PC 3 prostate cancer cell proliferation . ^^^ Co treating PC 3 cells with quercetin significantly attenuated EGF and TGF alpha induced growth and phosphorylation of ErbB 2 , ErbB 3 , c Raf , MAPK kinase 1 / 2 ( MEK1 / 2 ) , MAPK , Elk 1 and Akt 1 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Extravillous trophoblast differentiation in anchoring villi from uterine and ectopic pregnancies exhibited a comparable phenotypical switch : alpha 6 integrin subunit , E cadherin , EGF receptor , Ki 67 and connexin 40 were localized in the proximal part of the TCC , while alpha5beta1 and alpha 1 integrins , c erb B 2 , hPL and HLA G were expressed by invasive cytotrophoblasts . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Furthermore , expression of ErbB 1 alone or in combination with ErbB 2 in NIH3T3 cells up regulates Mcl 1 following EGF treatment . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Signal transduction by growth factor receptors , including HER 2 / neu and epidermal growth factor ( EGF ) receptors , can alter the phosphorylation of estrogen receptor ( ER ) and the biologic activity of ER dependent signaling networks both in the presence and in the absence of estrogenic ligands . ^^^ Sensitive and reliable assays of estrogen , HER 2 / neu , and EGF receptors and tumor associated angiogenesis will be important biologic factors to consider in the choice of optimal antitumor therapies for patients with breast cancer . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
We have investigated the protein expression pattern of the four ErbB receptors EGFR , Her 2 / neu , Her 3 and Her 4 , and correlated it with their putative ligands EGF , TGF alpha and HRG in 74 women with invasive breast cancer . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
One avenue by which HER 2 overexpression may dysregulate EGFR mediated cell responses , such as proliferation and migration , downstream of EGF family ligand binding , is by its modulation on EGFR endocytic trafficking dynamics . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
On the other hand , trials with EGF receptor inhibitors in unselected populations have shown anywhere from modest to no clinical activity . 1 will contrast below aspects in the development of inhibitors of Abl , c Kit , HER2 / neu ( erbB 2 ) , and EGFR , highlight successes and pitfalls in this field , and propose some approaches for the future development of tyrosine kinase inhibitors in human cancer . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
EGF stimulation of serum starved cells increased erbB 2 tyrosine phosphorylation and retarded erbB 2 migration in SDS PAGE , suggesting receptor association and activation . ^^^ Heregulin alpha 1 and beta 1 , two EGF related factors , had no effect on erbB 2 or EGFR phosphorylation . ^^^ This is the first time in which erbB 2 is reported to be expressed in an adipocytic cell line which does not depend on non EGF family growth factors ( thyroid hormone , growth hormone , etc . ) to accomplish adipose differentiation . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Overexpression of human EGF receptor HER 2 ( erbB 2 ) leads to amplified heregulin ( HRG ) signaling , promoting more aggressive breast cancer that is nonresponsive to estrogen and the antiestrogenic drug tamoxifen . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
HER 2 , a member of the human epidermal growth factor ( EGF ) receptor family , not only plays important roles in the progression of breast cancer tumorigenesis and metastasis , but may protect cancer cells from conventional cytotoxic therapies as well . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Thus , signal transduction via EGFR and erbB 2 could be related to the estrogen induced vaginal changes and persistent erbBs phosphorylation and sustained expression of EGF like growth factors , leading to ERalpha activation that may result in cancerous lesions in vaginae from neonatally DES exposed mice later in life . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
HER 2 / neu ( also known as HER 2 or c erb B 2 ) is a 185 kDa protein receptor with tyrosine kinase activity and extensive homology to the epidermal growth factor ( EGF ) receptor . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
NTAK ( neural and thymus derived activator for ErbB kinases ) , also known as neuregulin 2 , is a member of the epidermal growth factor ( EGF ) family , which binds directly to ErbB 3 and ErbB 4 and transactivates ErbB 2 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
GM 3 content modulates the EGF activated p185c neu levels , but not those of the constitutively activated oncoprotein p185neu . ^^^ In the former , p 185 ( c neu ) expression and activation are controlled by EGF addition to the culture medium and by epidermal growth factor receptor ( EGFR ) activity , whereas the latter express unchangingly high levels of constitutively activated p 185 ( neu ) . ^^^ In HC 11 cells treated with only [ D ] PDMP , p 185 ( c neu ) levels remain similar to control cells , whereas levels of tyrosine phosphorylated p 185 ( c neu ) increase after treatment with [ D ] PDMP in combination with EGF . ^^^ When exogenous GM ( 3 ) is added in combination with [ D ] PDMP and EGF , the enhanced phosphorylated p 185 ( c neu ) returns to control levels . ^^^ Interestingly , EGFR levels also vary and , analogously to phosphorylated p 185 ( c neu ) , the increase of EGFR content consequent to the [ D ] PDMP and EGF addition is reversed by exogenous GM ( 3 ) . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
In contrast to the epidermal growth factor ( EGF ) receptor , ErbB 2 is known to remain at the plasma membrane after ligand binding and dimerization . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
When erbB 2 monomeric yellow fluorescent protein ( mYFP ) or erbB 3 monomeric Citrine ( mCitrine ) were coexpressed with erbB 1 , the rates and extent of endocytosis of EGF QD and the RTK VFP demonstrated that erbB 2 but not erbB 3 heterodimerizes with erbB 1 after EGF stimulation , thereby modulating EGF induced signaling . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
We used the epidermal growth factor ( EGF ) receptor family members ErbB 1 and ErbB 2 as model systems to investigate whether the sugar moiety can be exploited to block signaling by growth factor receptors in human tumor cells ( i . e . , SKBR 3 and A 431 , derived from a breast cancer and a vulval carcinoma , respectively ) . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Each contains a mucin subunit with antiadhesive activity linked to the plasma membrane by means of a transmembrane subunit with two epidermal growth factor ( EGF ) like domains that act as ligand for ErbB 2 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
We compared the utilization of ErbB 2 in response to epidermal growth factor ( EGF ) and heregulin stimulation in colon carcinoma cells . ^^^ Anti ErbB 2 monoclonal antibody 2C4 blocked heregulin stimulated phosphorylation of ErbB 2 and ErbB 3 ; activation of mitogen activated protein kinase ( MAPK ) , phosphatidylinositol 3 ' kinase ( PI3K ) , and Akt ; proliferation ; and anchorage independent growth . 2C4 blocked EGF mediated phosphorylation of ErbB 2 and inhibited PI3K / Akt and anchorage independent growth but did not affect ErbB 1 or MAPK . ^^^ Furthermore , we identify ErbB 2 as a critical component of EGF signaling to the Gab1 / Gab2 PI3K Akt pathway and anchorage independent growth , but EGF stimulation of MAPK and monolayer growth can occur efficiently without the contribution of ErbB2 . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Our study suggests that residual ErbB 2 activation by EGF , despite EGFR blockade , is responsible for persistent downstream activation of Stat 3 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
We have also shown that conserved residues previously shown to be necessary for EGF receptor homodimerization may be dispensible for ErbB 2 ErbB3 heterodimerization . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
However , mutations within some of these receptors , and recent studies with the epidermal growth factor ( EGF ) and ErbB 2 receptors have indicated that interactions between TM domains do contribute to stabilization of ligand independent and / or ligand induced receptor dimerization and activation . ^^^ To test this hypothesis , we constructed expression vectors encoding short fusion peptides encompassing native or mutated TM domains of the EGF , ErbB 2 , and insulin receptors . ^^^ In human cell lines overexpressing the wild type EGF receptor or ErbB 2 , we observed that the peptides are expressed at the cell surface and that they inhibit specifically the autophosphorylation and signaling pathway of their cognate receptor . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Cell proliferation , AR , ER alpha , EGFR , ErbB 2 , EGF , IGF 1R , IGF 1 , VEGFR 2 , ERKs 1 and 2 proteins and TGF alpha mRNA , but not ER beta and VEGF , were significantly increased in prostates of TRAMP compared to C57BL / 6 mice . ^^^ Genistein in the diet significantly down regulated cell proliferation , EGFR , IGF 1R , ERK 1 and ERK 2 , but not AR , ER alpha , ER beta , ErbB 2 , EGF , TGF alpha , IGF 1 , VEGF and VEGFR in prostates of TRAMP mice . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
METHODS : MCF 7 breast cancer cells , which express high levels of AIB 1 , and a tamoxifen resistant derivative cell line engineered to overexpress HER 2 ( MCF 7 / HER2 18 ) were treated with estrogen , tamoxifen , epidermal growth factor ( EGF ) , or heregulin in the absence or presence of the EGF receptor ( EGFR ) tyrosine kinase inhibitor gefitinib . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
In SK OV 3 human ovarian cancer cells and SK BR 3 human breast carcinoma cells , ZH 4B blocks epidermal growth factor ( EGF ) induced phosphorylation of ErbB 2 in a dose dependent manner . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Here we tested whether the related human LRIG 1 ( also called Lig 1 ) protein can act as a negative regulator of EGF receptor and its relatives , ErbB 2 , ErbB 3 , and ErbB 4 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
However , expression levels for EGF receptor , erbB 2 and erbB 3 in specialized columnar epithelium ( SCE ) of Barrett ' s esophagus have yet to be determined . ^^^ To investigate the relationship between EGF family receptors and Barrett ' s esophagus , we examined expression levels for EGF receptor , erbB 2 and erbB 3 in SCE of Barrett ' s esophagus . ^^^ Biopsy samples of Barrett ' s mucosa stained or not stained with methylene blue applied endoscopically were used for histological evaluation and Western blot analysis of EGF receptor , erbB 2 and erbB 3 proteins . ^^^ In the Barrett ' s group , Western blot analysis showed that EGF receptor and erbB 2 were equally and strongly expressed in SCE and squamous epithelium ; in contrast , erbB 3 expression in SCE was considerably weaker . ^^^ Immunohistochemical analysis of SCE in Barrett ' s esophagus showed positive EGF receptor and erbB 2 expression , and no erbB 3 expression . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
ErbB 2 is a member of the EGF receptor tyrosine kinase family and has been rigorously studied as a signaling molecule on the cell membrane . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
In an effort to elucidate the role of ErbB receptors in human head and neck squamous cell carcinoma ( HNSCC ) , expression abnormalities and subcellular localization of epidermal growth factor receptor ( EGFR ) , ErbB 2 , ErbB 3 , and ErbB 4 were investigated along with EGF and tenascin by immunohistochemistry in 38 carcinomas as compared to adjacent normal mucosa of 24 cases . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Next , we analyzed expression levels of phosphorylated ERK1 / 2 and compared these results with EGFR ( HER 1 / ErbB1 ) and HER2 / neu ( ErbB 2 ) expression levels , as EGFR conducts signals through Ras Raf MAPK pathway ; gefitinib inhibited phosphorylation of ERK1 / 2 by EGF addition in cell lines with detectable and undetectable EGFR expression . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Over the past year , the field has progressed from having a complete absence of 10 ray crystal structures to having eight such structures ; ErbB 2 alone or complexed with the two monoclonal antibodies pertuzumab ( Genentech Inc / Roche Holdings AG / Chugai Pharmaceutical Co Ltd ) and trastuzamab , ErbB 3 without a ligand , EGFR with a ligand bound in an unactivated monomeric conformation , and EGFR with either epidermal growth factor ( EGF ) or transforming growth factor alpha ( TGFalpha ) in a 2 : 2 dimeric complex . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Stimulation of the duct cells with epidermal growth factor ( EGF ) and betacellulin results in Tyr phosphorylation of ErbB 1 and ErbB 2 , followed by activation of Shc , MEK1 / 2 and ERK1 / 2 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
PURPOSE : Human corneal epithelial cells ( HCECs ) were functionally depleted of erbB 2 to elucidate its role in epidermal growth factor ( EGF ) receptor ( EGFR ) activation dependent cell migration . ^^^ Effects of erbB 2 depletion on heparin binding EGF like growth factor ( HB EGF ) induced cell migration were determined by Boyden chamber migration assay and by scratch wound assay . ^^^ Depletion of functional erbB 2 attenuated the healing of scratch wounds in the presence of HB EGF and impaired both chemotactic migration stimulated by HB EGF and haptotactic migration toward a fibronectin collagen 1 ( 3 : 1 ; FNC ) coating mix . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The epidermal growth factor system has been associated to prognosis in patients with bladder cancer based mainly on the expression of the epidermal growth factor ( EGF ) receptor 1 ( EGFR ) and HER 2 and their activating ligands . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
This regulation involves the EGF receptor , possibly ErbB 2 that is highly expressed in SK BR 3 cells , and a PI3K / JNK pathway . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Thereafter , high levels of FAS are maintained in coordination with increased demand for fatty acid metabolism and / or membrane synthesis in response to cancer related overexpression of growth factors ( e . g . , EGF , heregulin ) and / or growth factor receptors ( e . g . , EGFR , Her 2 / neu ) . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
INTRODUCTION : Insulin like growth factor binding protein 3 ( IGFBP 3 ) differentially modulates breast epithelial cell growth through insulin like growth factor ( IGF ) dependent and IGF independent pathways and is a direct ( IGF independent ) growth inhibitor as well as a mitogen that potentiates EGF ( epidermal growth factor ) and interacts with HER 2 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The ErbB family , ErbB 1 ( also known as the epidermal growth factor receptor EGFR ) , ErbB 2 , ErbB 3 , and ErbB 4 comprise a group of receptor tyrosine kinases that interact with ligands from the epidermal growth factor ( EGF ) superfamily , subsequently dimerize , catalytically activate each other by cross phosphorylation , and then stimulate various signaling pathways . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Other studies also correlate EGF receptor ( EGFR ) expression with poor prognosis in breast cancer , but follow up studies suggest that this association is much less robust than with ErbB 2 , and requires more careful analysis . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
EGF R and erbB 2 in murine tooth development after ethanol exposure . ^^^ Epidermal growth factor receptor type 1 ( EGF R ) and epidermal growth factor receptor type 2 ( erbB 2 ) immunoexpression during the lower first molar morphogenesis was investigated in mouse fetuses exposed to ethanol during gestation . ^^^ Immunohistochemistry was applied to EGF R and erbB 2 . ^^^ RESULTS : At days 16 . 5 and 18 . 5 , fetuses from 15 % , 20 % , and 25 % ethanol groups showed delayed differentiation , degenerative changes in dental epithelial tissues and reduced dental size ; additionally , they displayed an enhanced immunoreactivity to EGF R and erbB 2 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
After 4 hours of exposure , cetuximab reduced epidermal growth factor ( EGF ) induced phosphorylation of EGFR ( pEGFR ) and HER 2 ( pHER 2 ) in cetuximab sensitive cell lines but not in cetuximab resistant cell lines . ^^^ In the absence of EGF , cetuximab alone increased the level of pEGFR and pHER 2 above that seen in untreated control cells in both sensitive and resistant cell lines that were EGFR and HER 2 positive , but not in EGFR or HER 2 negative lines . ^^^ Despite the cetuximab induced increase in phosphorylation of EGFR and HER 2 , peak EGF induced levels of pEGFR and pHER 2 were reduced by cetuximab in the cetuximab sensitive lines but not in the resistant lines . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Phosphorylation of both EGFR and ErbB 2 is a reliable predictor of prostate cancer cell proliferation in response to EGF . ^^^ ErbB 2 recruitment potentiated EGF induced signals and was associated with the most pronounced effects of EGF despite low EGFR expression . ^^^ In this way , the sum of EGFR and ErbB 2 receptor phosphorylation proved to be a more sensitive indicator of EGF induced proliferation than quantification of the expression of either receptor alone . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
We also found that arsenite stimulated EGF ( ErbB 1 ) and ErbB 2 receptor transactivation , manifest as receptor tyrosine phosphorylation , appeared to be a proximal signaling event leading to p21Cip1 / Waf1 induction , because both pharmacological inhibitors and knockdown of receptors by RNA interference blocked arsenite induced p21Cip1 / Waf1 upregulation . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
RESULTS : EGF induced EGFR / EGFR and EGFR / c erbB 2 interactions correlate with stimulation of cell proliferation in BT 474 cells . ^^^ In SK BR 3 Herceptin treatment causes c erbB 2 phosphorylation of Y 877 and Y 1248 , EGF induces Y 877 and Y 1112 phosphorylation . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
GHRH and BN / GRP antagonists reduced bFGF concentrations and the maximal binding capacity of EGF receptors , and their mRNA levels in PC 3 and DU 145 tumors . mRNA levels for HER 2 and 3 were also diminished in PC 3 tumors by GHRH and BN / GRP antagonists . ^^^ CONCLUSIONS : BN / GRP and GHRH antagonists inhibit growth of PC 3 and DU 145 prostate cancers by suppressing the expression of tumoral growth factors such as VEGF and bFGF as well as the receptors for EGF and related HER 2 and 3 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The expression of EGF receptors ( ErbB 1 and ErbB 2 , but not ErbB 3 or ErbB 4 ) was down regulated significantly in the same forebrain regions . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Growth factors and their receptors , cell cycle regulators , cell adhesion molecules and matrix degrading enzymes are those to be used as prognostic factors , including epidermal growth factor ( EGF ) , EGF receptor , K sam , HER 2 , interleukin ( IL ) 8 , vascular endothelial growth factor ( VEGF ) , cyclin E , p 27 , E cadherin , CD44v6 , matrix metalloproteinase 1 ( MMP 1 ) , and tissue inhibitor of matrix metalloproteinase 1 ( TIMP 1 ) . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Endogenous RPTPkappa associated with EGF receptor and HER 2 , resulting in suppression of basal and ErbB ligand induced proliferation and receptor phosphorylation . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Treatment of astrocytes , however , with heregulin , which signals through other members of the EGF receptor family ( ErbB 2 , ErbB 3 , ErbB 4 ) , did not induce CSPG upregulation . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
This review focuses on a discussion of tyrosine kinases thought to be important in disease , including platelet derived growth factor ( PDGF ) , fibroblast growth factor ( FGF ) , vascular endothelial cell growth factor ( VEGF ) , epidermal growth factor ( EGF ) receptors , HER 2 and Src . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Neuregulins constitute a family of EGF like ligands that bind to HER 3 or HER 4 , preferably forming heterodimers with the orphan receptor HER 2 . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Human EGF Receptor 1 ( HER 1 ) showed highest expression during the proliferative phase , HER 2 and HER 4 during the early and HER 3 during the late secretory phase . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Our results demonstrated that ( 1 ) EGFR kinase can efficiently phosphorylate a broad range of diverse peptide sequences representing ErbB sites ; ( 2 ) certain ErbB 2 , ErbB 3 and ErbB 4 sites had higher specificity constants than any EGFR sequence and ( 3 ) EGF stimulation consistently increases the k ( cat ) approx . 5 fold , but does not significantly alter the K ( m ) for any ErbB peptides . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
We have recently shown that peptides corresponding to the transmembrane domains of the epidermal growth factor ( EGF ) and ErbB 2 receptors inhibit their corresponding receptor activation in cancer cell lines . ^^^ Peptides corresponding to the transmembrane domains of the EGF receptor and ErbB 2 are able to inhibit specifically the autophosphorylation of insulin receptors with the corresponding domain . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
They expressed epidermal growth factor ( EGF ) receptor and HER 2 on their surface and accordingly , their growth was significantly stimulated by EGF , transforming growth factor ( TGF ) alpha and heregulin . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
A literature search was undertaken using PubMed and MEDLINE search engines , using the keywords p 53 , p 21 , p 16 , p 27 , SMAD 4 , K ras , cyclin D 1 , Bax , Bcl 2 , EGFR , EGF , c erbB 2 , HB EGF , TGFbeta , FGF , MMP , uPA , cathepsin , heparanase , E cadherin , laminins , integrins , TMSF , CD 44 , cytokines , angiogenesis , VEGF , IL 8 , beta catenin , DNA microarray , and gene profiling . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
BACKGROUND : ErbB 2 ( HER 2 ) , a member of the epidermal growth factor ( EGF ) receptor family , is a class 1 transmembrane receptor tyrosine kinase . ^^^ We used EGF and trastuzumab covered paramagnetic microspheres , quantitative confocal laser scanning microscopy , and digital image processing to investigate the ( trans ) activation of and local signal propagation from erbB 1 and erbB 2 on trastuzumab sensitive and resistant carcinoma cell lines expressing these receptors at high levels . ^^^ RESULTS : On A 431 cells expressing high levels of endogenous erbB 1 and transfected erbB 2 mYFP ( A 4 erbB2 mYFP F 4 cell line ) , EGF coupled microspheres activated erbB 1 and transactivated erbB 2 mYFP . ^^^ In two other cell lines with comparable erbB 2 expression but lower levels of erbB 1 , EGF microspheres transactivated erbB 2 less efficiently . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
However , coexpression of HER 2 with the HPV 16 oncoproteins E 6 and E 7 resulted in EGF independent cells that expressed very high levels of constitutively activated HER 2 . ^^^ Interestingly , coexpression of E 7 with HER 2 resulted in cells that were EGF independent for growth but did not express HER 2 to high levels , and coexpression of E 6 with HER 2 resulted in cells expressing higher levels of HER 2 , which were still dependent on EGF for growth and survival . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
By constructing stably transfected cells harboring the same amount of epidermal growth factor ( EGF ) receptor ( EGFR ) , but with increasing overexpression of ErbB 2 , we have demonstrated that ErbB 2 efficiently inhibits internalization of ligand bound EGFR . ^^^ Apparently , ErbB 2 inhibits internalization of EGF bound EGFR by constitutively driving EGFR ErbB 2 hetero / oligomerization . ^^^ Instead , we demonstrate that in contrast to EGFR homodimers , which are capable of inducing new clathrin coated pits in serum starved cells upon incubation with EGF , clathrin coated pits are not induced upon activation of EGFR ErbB 2 heterodimers / oligomers . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Overexpression of ADAMTS 1 in these cells promoted tumor angiogenesis and invasion , shedding of the transmembrane precursors of heparin binding epidermal growth factor ( EGF ) and amphiregulin ( AR ) , and activation of the EGF receptor and ErbB 2 , while overexpression of ADAMTS 1E / Q inhibited these events . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Among ErbB tyrosine kinase receptors , EGF receptor ( EGFR ) and ErbB 2 were selectively activated by tRA treatment in skin from wild type mice , while the activation of these ErbB receptors was significantly reduced in the skin of HB EGF null mice . ^^^ These results indicate that expression of HB EGF and generation of its soluble form , followed by activation of EGFR and ErbB 2 , are pivotal processes in tRA induced epidermal hyperplasia . . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
ErbB 2 , a member of the EGF receptor family of tyrosine kinases is overexpressed on many tumor cells of epithelial origin and is the molecular target of trastuzumab ( Herceptin ) , the first humanized antibody used in the therapy of solid tumors . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
We studied epithelial expression of EGF , transforming growth factor a , amphiregulin , heregulin ( HRG ) , betacellulin ( BTC ) , and their receptors , EGFR , HER 2 , and HER 3 , by immunohistochemical analysis in resected bronchial tissue from 20 subjects with ( forced expiratory volume in 1 second [ FEV ( 1 ) ] < 75 % of predicted value ) and 18 without ( FEV ( 1 ) > 85 % predicted value ) COPD . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Six genes were over expressed ( ERBB 2 , TGFA , HB EGF , AREG , EREG and EPGN ) , 3 were under expressed ( ERBB 1 , ERBB 4 and NRG 4 ) and 2 were over or under expressed ( ERBB 3 and BTC ) . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
As a consequence of their expressing EGFR , tumor endothelial cells responded to EGF and other EGF family members by activating both EGFR and ErbB 2 , by activating the downstream mitogen activated protein kinase pathway , and by enhanced proliferation . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Benefit from adjuvant tamoxifen therapy in primary breast cancer patients according oestrogen receptor , progesterone receptor , EGF receptor and HER 2 status . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
HB EGF signaling through ErbB 2 is essential for the maintenance of homeostasis in the adult heart , whereas HB EGF signaling through EGFR is required during cardiac valve development . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The early peak of relapse in patients with breast carcinomas that overexpress HER 2 oncoprotein and dissemination to the axillary lymph nodes might be related to proliferation of micrometastatic lesions induced by EGF family growth factors released at the time of surgery . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Increased expression of the epidermal growth factor ( EGF ) receptors , HER 1 and HER 2 are related to poor prognosis in most cancers studied . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
We further demonstrate that a combination of DNA vaccines encoding Amot and the extracellular and transmembrane domains of the human EGF receptor 2 ( Her 2 ) / neu oncogene inhibited breast cancer progression and impaired tumor vascularization in Her 2 / neu transgenic mice . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The emergence of ErbB 2 expression in cultured rat hepatocytes correlates with enhanced and diversified EGF mediated signaling . ^^^ Although ErbB 2 was not present in freshly isolated hepatocytes , EGF and insulin independently induced ErbB 2 while suppressing ErbB 3 expression . ^^^ Low concentrations of EGF and insulin synergistically stimulated ErbB 2 expression and DNA synthesis . ^^^ EGF promoted EGFr and ErbB 2 coassociation , stimulating tyrosine phosphorylation of both proteins . ^^^ A selective tyrphostin inhibitor of ErbB 2 suppressed EGF stimulated DNA synthesis , but maximum suppression required the blockade of the EGFr kinase as well . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Her2 / neu ( Her 2 ) is a tyrosine kinase belonging to the EGF receptor ( EGFR ) / ErbB family and is overexpressed in 20 30 % of human breast cancers . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
Hsp 90 client proteins include telomerase , mutated p 53 , Bcr Abl , Raf 1 , Akt , HER2 / Neu ( ErbB 2 ) , mutated B Raf , mutated EGF receptor , and HIF 1alpha . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
The immunohistochemistry method was used to observe the number of G cells , the expression of protein of EGFR ( EGF receptor ) , C erbB 2 , p 53 , p 16 and bcl 2 in gastric tissue . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
To go further in the characterization of the EGF pathway , we screened EGFR , ERBB 2 , ERBB 3 , KRAS , BRAF , and PIK3CA for mutations in 2 groups of White patients with nonsmall cell lung cancer ( 45 cancers from women and 46 cancers from men ) . ^^^
Interacting proteins: P01133 and P04626	Pubmed	SVM Score :0.0
To address this problem , we use quantitative mass spectrometry to analyze dynamic effects of HER 2 overexpression on phosphotyrosine signaling in human mammary epithelial cells stimulated by epidermal growth factor ( EGF ) or heregulin ( HRG ) . ^^^ Data generated from this analysis reveal that EGF stimulation of HER 2 overexpressing cells activates multiple signaling pathways to stimulate migration , whereas HRG stimulation of these cells results in amplification of a specific subset of the migration signaling network . ^^^ Self organizing map analysis of the phosphoproteomic data set permitted elucidation of network modules differentially regulated in HER 2 overexpressing cells in comparison with parental cells for EGF and HRG treatment . ^^^