Pubmed abstracts for Protein-Protein Interaction search result :


Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
Isolation and characterization of the major gel proteins in human semen , semenogelin 1 and semenogelin 2 . ^^^ Semenogelin 1 and semenogelin 2 constitute the major gel forming proteins in human semen . ^^^ Approximately 10 20 mg semenogelin 1 and 2 5 mg semenogelin 2 were recovered from each sample with a purity exceeding 95 % as judged by SDS / PAGE . ^^^ The molecular mass of semenogelin 1 ( 49 958 Da ) and the major form of semenogelin 2 ( 63 539 Da ) measured by mass spectrometry was consistent with the reported cDNA data . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
Semenogelin 1 and semenogelin 2 , the major gel forming proteins in human semen , are substrates for transglutaminase . ^^^ The major seminal vesicle secreted proteins in human semen , semenogelin 1 and semenogelin 2 , interact non covalently and via disulphide bridges to instantly form a coagulum upon ejaculation . ^^^ However , we here show that semenogelin 1 and semenogelin 2 , both in seminal vesicle fluid and purified from semen , are substrates for factor XIIIa , the fibrin cross linking transglutaminase . ^^^ Purified semenogelin 1 and semenogelin 2 could be cross linked separately into complexes . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
The cotton top tamarin carries an extended semenogelin 1 gene but no semenogelin 2 gene . ^^^ This gene has now been cloned and identified as a semenogelin 1 gene , because of a higher number of conserved nucleotides in the human semenogelin 1 gene ( 89 % ) than in the human and the rhesus monkey semenogelin 2 genes ( 82 % ) . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
It is secreted as biologically active 52 kDa and a mixture of 71 and 76 kDa precursor forms , which are identical to semenogelin 1 and 2 ( Sg 1 and Sg 2 ) , respectively . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
METHODS : The proteolysis of human semenogelin 1 ( SgI ) and 2 ( SgII ) by PSA was characterized by purification of generated SgI and SgII fragments , N terminal sequencing , and mass spectrometry . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
Two proteins recovered from cell surface adhesion complexes in a small cell lung carcinoma ( SCLC ) cell line were identified as fragments of the seminal plasma proteins semenogelin 1 and semenogelin 2 . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
The human seminal vesicles secrete two proteins , semenogelin 1 and semenogelin 2 , at very high concentrations . ^^^ We have now determined the nucleotide sequence of DNA located 5 13 kilobases ( kb ) downstream of the tamarin semenogelin 1 gene a region that in man is occupied by the semenogelin 2 gene . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
Semenogelin 1 ( SgI ) and semenogelin 2 ( SgII ) are the dominating protein components of the coagulum formed by freshly ejaculated human semen . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
Human semen coagulum predominantly consists of high molecular mass complexes of the seminal vesicle secreted semenogelin 1 ( SgI ) and semenogelin 2 ( SgII ) . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
On the serological relationship of fimbrial antigens between salmonella SGI and Yersinia enterocolitica and Salmonella SG 2 and Arizona ( = Salmonella SG 3 ) ( author ' s transl ) ] . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
SgII , which has 78 % overall identity with SgI , contains eight 60 residue regions that display conspicuous internal sequence similarity , whereas SgI only contains six of these regions . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
This new sub subgel ( SGII ) phase may be a precursor to the subgel ( SGI ) phase , and this discovery is discussed in relation to the current knowledge regarding the polymorphic gel phases of both ester and ether linked lipids with identical acyl chains . . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
A single amino acid mutation at positions 172 ( Met to Ala ) or 305 ( Asn to Ala ) was sufficient to change the subgroup specificity of the human rotavirus Wa VP 6 protein from SGII to SGI / II , since either of these mutations allowed the protein to be recognized by the SGI mAb 255 / 60 , while retaining its capacity to interact with the SGII mAb 631 / 9 . ^^^ In the case of the SGII epitope , the mutation of two contiguous amino acids ( Ala 305 Asn306 to Asn 305 Ala306 ) in the porcine rotavirus YM VP 6 protein ( SGI ) enabled the protein to be efficiently recognized by the SGII mAb 631 / 9 , while causing the YM VP 6 protein to lose its capacity to interact with mAb 255 / 60 . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
The SG and VP 7 ( G ) serotype specificities could be determined for 118 ( 84 % ) of the 140 rotavirus positive stool specimens ; 4 rotavirus strains were serotype G 1 and SGII ; 1 strain was serotype G 2 and SGI+II ; 112 strains were serotype G 3 and SGII ; 1 strain was serotype G 3 and SGI ; and none of the strains was serotype G 4 . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
A comparison of the SgI gene and the SgII gene suggests that they evolved by the duplication of an approximately 8 kb DNA segment about 61 million years ago , probably by a mechanism involving recombination between L 1 elements . . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
SGI and SGII specificity were detected in 60 per cent and 20 per cent of the cases studied . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
We examined CgA , SgI and SgII mRNA expression by reverse transcription polymerase chain reaction in rat mammary adenocarcinoma and fibroadenoma which had arisen spontaneously in aging Fischer 344 rats . ^^^ CgA and SgII mRNA were expressed in both mammary tumors , but SgI mRNA was not detected in either . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
Linkage between NSP 4 genotypes and VP 6 subgroups was 100 % , NSP 4 genotype A being linked to VP 6 of subgroup 1 ( SGI ) and NSP 4 of genotype B being linked to VP 6 of SGII . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
Human seminal plasma contains high concentrations of prostatic acid phosphatase ( PAP ) , prostate specific antigen ( PSA ) , beta microseminoprotein ( MSP ) , semenogelin 1 ( SgI ) , and semenogelin 2 ( SgII ) , whereas only PAP and MSP are present in rodents . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
Western blots detected semenogelin 1 and 2 in both RPE and neural retina while the vitreous contained only SgII . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
Among these specimens , we identified both the four globally common strains ( P [ 8 ] , G 1 subgroup 2 ( sgII ) ; P [ 4 ] , G 2 sgI ; P [ 8 ] , G 3 sgII ; and P [ 8 ] , G 4 sgII ) and six uncommon strains ( P [ 6 ] , G 4 sgII ; P [ 9 ] , G 3 sgI ; P [ 9 ] , G 6 sgI ; P [ 14 ] , G 6 sgI ; P [ 8 ] , G 9 sgII ; and P [ 8 ] , G 9 sgI ) . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
In semen , the gel proteins SgI and SgII ( semenogelins 1 and 2 ) are digested by PSA ( prostate specific antigen ) , resulting in liquefaction and release of motile spermatozoa . ^^^ We characterized the binding of Zn2+ to SgI and SgII and found evidence that these proteins are involved in regulating the activity of PSA . ^^^ Intact SgI and SgII and synthetic semenogelin peptides were used in the experiments . ^^^ SgI and SgII bound Zn2+ with a stoichiometry of at least 10 mol ( mol of protein ) ( 1 ) and with an average dissociation constant of approx . 5 microM per site . ^^^ Moreover , Zn2+ inhibited PSA was activated by exposure to SgI or SgII . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
Here we examine the molecular evolution of the semenogelin genes ( SEMG 1 and SEMG 2 ) , which code for the predominant structural proteins in human semen . ^^^ In contrast , at both SEMG 1 and SEMG 2 we observed several gorilla haplotypes that contain at least one premature stop codon . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
Urinary sialoglucides were fractionated into two fractions ( SG 1 and SG 2 ) by Sephadex G 25 gel filtration and considerable increase in excretion of SG 1 was observed in the patients with lysosomal diseases : two to three fold increase in mucopolysaccharidosis type 2 and seven to eight fold increase in mucolipidosis . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
They were named SG 1 for the new toxin and SG 2 for the ciguatoxin like compound . ^^^ Successive filtrations on Sephadex LH 20 of SG 1 and SG 2 gave respectively a lethality to mice of 0 . 03 microgram / g and 0 . 06 microgram / g . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
The four groups included boars from a synthetic line ( SG 1 ) or the Large White breed ( SG 2 ) and barrows from the Large White breed ( SG 3 ) or crossbred between Large White and Meishan breeds ( SG 4 ) . ^^^ In E1 / 0 pigs , daily BW , lean , and N gain were affected ( P less than . 01 ) by group ; 566 , 471 , 374 , and 315 g / d of lean tissue gain in SG 1 , SG 2 , SG 3 , and SG 4 pigs , respectively . ^^^ At high ME intake ( E2 / pST vs E1 / 0 ) , pST increased daily BW ( +14 % ) , lean ( +27 % ) , or N ( +26 % ) gain and reduced adipose tissue ( 50 % ) gain , but the pST effect was inversely related to LTG : for N , the improvement was 2 . 8 , 7 . 1 , 7 . 0 , and 11 . 1 g / d in SG 1 , SG 2 , SG 3 , and SG 4 groups , respectively . ^^^ Energy restriction ( E3 / pST vs E2 / pST ) reduced ( P less than . 001 ) adipose tissue gain in all groups but did not affect lean tissue or N gains in SG 1 , SG 2 , and SG 3 pigs . ^^^ Energy restriction combined with pST treatment ( E3 / pST ) led to negligible amounts of fat deposited ( 40 g / d for SG 1 + SG 2 + SG 3 pigs ) and a gain : feed ratio higher than 500 g / kg ( 580 in SG 1 pigs ) . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
Long pattern isolates usually belong to subgroup 2 ( SG 2 ) and short pattern to subgroup 1 ( SG 1 ) . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
Monoclonal antibodies specific for rotavirus subgroup 1 ( SG 1 ) and subgroup 2 ( SG 2 ) were used to analyse by enzyme immunoassay ( EIA ) the subgroups of human rotavirus isolates obtained from three different parts of India during the period September 1985 to July 1987 . ^^^ We identified one isolate which failed to react with either SG 1 or SG 2 specific monoclonal antibodies , although it reacted well with a monoclonal antibody specific for group A rotaviruses . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
Two monoacidic gangliosides were isolated and purified in this study and designated as sperm ganglioside 1 and 2 ( sg 1 and sg 2 ) . ^^^ The two gangliosides , sg 1 and sg 2 , contained the same neutral sugars , galactose and glucose in molar ratio of 1 : 1 and no GalNAc except for the presence of N acetyl neuraminic acid ( NeuAc ) in sg 1 and deaminated neuraminic acid ( KDN ; 2 keto 3 deoxy D glycero D galacto nononic acid ) in sg 2 . ^^^ The complete structures of these gangliosides were determined by a combination of methylation analysis , fast atom bombardment mass spectrometry , 400 MHz one and two dimensional 1H nuclear magnetic resonance spectroscopy , fatty acid analysis , and endoglycoceramidase digestion NeuAc alpha 2 3Gal beta 1 4Glc beta 1 Cer sg 1 [ ( NeuAc ) GM 3 ] KDN alpha 2 3Gal beta 1 4Glc beta 1 Cer sg 2 [ ( KDN ) GM 3 ] where , for both sg 1 and sg 2 , the ceramide moieties ( Cer ) were found to be made up of 4 sphingenine and mainly C 16 : 0 fatty acid ( palmitate ; 95 % ) with a minor amount of C 24 : 1 fatty acyl chain ( nervonate , 5 % ) . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
In the present study , we investigated in GH4C1 cell cultures the regulation of the cell content of the Sg by immunoblotting and the relationship between the storage of Sg 1 and Sg 2 and PRL by double immunocytochemistry . ^^^ GH4C1 cells grown in the presence of gelded horse serum , a condition in which these cells contain a low level of secretory granules , contained low levels of PRL , Sg 1 , and Sg 2 . ^^^ Treatment of GH4C1 cells with a combination of 17 beta estradiol , insulin , and epidermal growth factor for 3 days , known to induce a marked increase in the number of secretory granules , increased the cell contents of PRL , Sg 1 , and Sg 2 . ^^^ Forty six percent of PRL positive cells stained coincidently for Sg 1 , while 72 % of the PRL cells were also reactive with anti Sg 2 . ^^^ To determine whether PRL storage was associated with storage of at least one of the Sg , cells were stained with anti PRL and anti Sg 1 and anti Sg 2 together . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
A comparison between the static grades and the diameter of the internal spermatic vein ( ISV ) showed that the mean value of the diameter of the ISV in SG 1 , SG 2 and SG 3 was 4 . 1 + / 0 . 66 mm , 5 . 1 + / 0 . 68 mm , and 6 . 2 + / 0 . 79 mm , respectively . ^^^ Patients with higher static grades had ISVs of significantly larger diameter ( SG 1 vs SG 2 ; p less than 0 . 05 , SG 2 vs SG 3 ; p less than 0 . 01 , SG 1 vs SG 3 ; p less than 0 . 01 , t test ) . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
Values at 60 min showed an adequate anticoagulation in only 6 patients ( 24 % ) in SG 1 vs 21 ( 84 % ) in SG 2 . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
We produced three monoclonal antibodies , SG 1 , SG 2 and SG 3 , specific for human seminal gamma glutamyltransferase when characterized by enzyme linked immunosorbent assay and immunoblotting . ^^^ Seminal gamma glutamyltransferase was localized , by immunostaining , to the epithelial cells of the ductus epididymidis , seminal vesicle and prostate gland with SG 1 , those of the prostate gland with SG 2 , and those of the seminal vesicle with SG 3 . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
The findings were classified into significance groups ( SG ) depending on their clinical relevance ( SG 1 : crucial ; SG 2 : of immediate relevance ; SG 3 : of future relevance ; SG 4 : of no significance ) . ^^^ In Group 2 , abnormalities were noted in 70 . 1 % of the patients , and were assigned to SG 1 in 4 . 3 % , SG 2 in 5 . 4 % , SG 3 in 32 . 5 % and SG 4 in 27 . 9 % . ^^^ After excluding the findings in the pleural cavities ( 14 . 1 % ) , aorta ( 3 . 4 % ) and biliary tract ( 1 . 2 % ) , most of the findings noted in other organs or regions of the body ( leg veins 11 . 1 % , intestine 8 . 8 % , spleen 8 . 3 % , peritoneal cavity 7 . 2 % , pancreas 1 . 7 % ) were assigned to SG 1 or SG 2 . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
In 19 out of 49 patients with legionellosis L . pneumophila SG 1 ( 3x ) , SG 2 ( 1x ) , SG 3 ( 4x ) , SG 4 ( 1x ) , SG 5 ( 5x ) , L . micdadei ( 3x ) and L . bozemanii ( 2x ) were found . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
As they could be diagnosed they belong to L . pneumophila SG 1 306 strains , SG 2 36 strains , SG 3 127 strains , SG 4 45 strain , SG 5 29 strain , SG 6 106 strains , SG 9 13 strains and SG 10 13 strains . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
Patients treated with antifibrinolytics were divided into two groups , according to the day of hospital admission and onset of therapy , respectively between 0 and 3 days ( SG 1 ) and between 4 and 7 days from SAH ( SG 2 ) ; treated patients ( 260 cases ) received i . v . tranexamic acid ( 6 gr / day ) for at least two weeks . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
It was admitted that if the areas among the particles of a size lower than 0 . 2 micron ( 200 nm ) were prevailing the granular matrix disorganization ( GMD ) was of type ( SG 1 ) ; if most of the areas were larger than 0 . 2 micron ( 200 nm ) GMD was of type 2 ( SG 2 ) and preceded the complete fusion of the granules . . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
Peripheral resistance decreased during both infusions in SG 1 but remained unaltered in CG 1 ( p less than 0 . 05 ) . 6 weeks after finishing anabolic steroid treatment ( SG 2 , n = 11 ) , in the CaCl 2 test the ejection fraction ( p less than 0 . 05 ) and stroke index were smaller than in control rats of the same age ( CG 2 , n = 12 ) . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
Dextrin dextranase ( EC 2 . 4 . 1 . 2 ; DDase ) , which is produced by Acetobacter capsulatus ATCC 11894 , acted on a mixture of stevioside and starch hydrolysate with isoamylase , so that the enzyme was found to convert stevioside to predominantly mono glucosyl stevioside ( SG 1 ) and di glucosyl stevioside ( SG 2 ) , and little of the stevioside initially added remained . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
The two sg RNAs are 2155 ( sg 1 ) and 934 ( sg 2 ) nt in length . ^^^ ORF 3 is expressed from sg 1 RNA whereas ORFs 4 and 5 are potentially expressed from sg 2 RNA . ^^^ Time course experiments with Cynara scolymus protoplasts indicate that during AMCV infection both positive and negative strands of genomic and sg RNAs are produced and that sg 2 RNA is produced before and at a higher level than sg 1 RNA . . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
We investigated the subcellular distribution of secretogranins 1 , 2 ( Sg 1 , Sg 2 ) , and prolactin ( PRL ) by double immunogold electron microscopy in GH3B6 rat pituitary tumor cells grown in different culture conditions and in normal PRL cells in adult male rat anterior pituitary . ^^^ Co localization of Sg 1 or Sg 2 with PRL was observed in most secretory granules in GH3B6 cells and normal PRL cells , except for some secretory granules containing only Sgs in GH3B6 cells and containing only PRL in normal PRL cells . ^^^ The present study indicates a heterogeneous subcellular distribution of PRL , Sg 1 , and Sg 2 in individual secretory granules of GH3B6 cells and of normal PRL cells , pointing out the formation of different types of aggregates during the condensation of secretory products in these two PRL cell models . . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
Follow up epidemiology of this event in Manipur during 1989 1992 yielded mainly rotaviruses of more conventional characteristics ( 94 isolates of SG 2 and long electropherotype , and 90 isolates of SG 1 and short electropherotype ) , but also 6 isolates of SG 1 with long electropherotype , indicating that these viruses continue to circulate in the Manipur community . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
Patients from SG 2 had pain for a longer time ( P < 0 . 01 ) compared to patients from SG 1 with normal movement and electromyographic profiles . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
During the study , 28 patients ( SG 0 ) received S 10mg ; in 14 patients ( SG 1 ) dosage was titrated to 20mg and in 18 cases ( SG 2 ) to 40mg . ^^^ RESULTS Mean profile analysis showed different behaviour of BG , SG 0 , SG 1 and SG 2 through time for TC , TG , LDL C and VLDL C . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
Three acidic sulfated proteins , chromogranin A ( Cg A ) , secretogranin 1 ( Sg 1 ; also called chromogranin B ; Cg B ) , and secretogranin 2 ( Sg 2 ) are well known as the major proteins in the family . ^^^ In this review , the possible roles of granin family , especially Cg A , Sg 1 ( Cg B ) and Sg 2 in the regulated secretory pathway are discussed , focusing on the sorting , aggregation of proteins in the TGN , and subsequent secretory granule formation . . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
When the derangement of cerebral perfusion was estimated with four SPECT grades ( SG ) , 70 % of their hemispheres revealed normal ( SG 1 ) or localized decreased perfusion ( SG 2 ) . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
Hypotension and bradycardia were the most frequent adverse effects . 4 . 3 % of the adverse effects were graded as minimal ( SG 1 ) , 2 . 4 % as mild ( SG 2 ) and 0 . 7 % as moderate ( SG 3 ) . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
The sori of Dictyostelium discoideum ( strains SG 1 , SG 2 , NC 4 and V 12 ) contained more than 100 mM ammonium phosphate . ^^^ An exogenous concentration of 69 mM ammonium phosphate could maintain dormancy in spores of strains SG 1 and SG 2 for at least a week in the absence of any other inhibitory component from the sori . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
Here we utilized the technique of acid activation to identify cryptic cysteine proteinases throughout auto induced and heat induced spore germination of D . discoideum strain SG 2 and SG 1 . ^^^ The major acid activatable cysteine proteinase identified in SG 2 and SG 1 spore extracts was ddCP 38 ( D . discoideum cysteine proteinase with a molecular mass of 38 kDa ) and ddCP 48 , respectively . ^^^ Moreover , we were able to detect these cryptic cysteine proteinases in the vegetative cells and early aggregates of both strains SG 1 and SG 2 . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
About 94 % of the samples ( 85 / 90 ) could be subgrouped with the following results : 55 . 5 % for SG 2 , 7 . 8 % SG 1 , and 31 . 1 % for SG non 1 non 2 . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
Spodoptera frugiperda larvae have three midgut alpha galactosidases ( SG 1 , SG 2 , SG 3 ) partially resolved by ion exchange chromatography . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
Sg consists of two proteins , one 52 kDa ( Sg 1 ) in size , and the other a mixture of 71 and 76 kDa proteins ( Sg 2 ) . ^^^ Recombinant Sg 1 and Sg 2 proteins were obtained using a baculovirus system and then injected into a rabbit to produce the respective antibodies [ Characterization of recombinant precursor proteins of the human seminal plasma sperm motility inhibitor synthesized in insect cells , Int . ^^^ When liquefied seminal plasma was immunoblotted with the anti Sg 1 and Sg 2 antibodies , the anti Sg 2 antibody identified a wider range of the polypeptides originating from Sg than did the anti Sg 1 antibody . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
The HCV genotype of the patients was determined by an enzyme linked immunosorbent assay based on serological genotype 1 and 2 specific recombinant peptides ( SG 1 and SG 2 , respectively ) of the NS 4 region . ^^^ The prevalence of SG 1 and SG 2 HCV was similar in the four main islands of Japan . ^^^ The percentage of patients with SG 1 HCV increased by 7 % , while that of patients with SG 2 HCV decreased by 7 % , as liver disease progressed in severity from chronic hepatitis to carcinoma ( P < 0 . 001 ) . ^^^ Patients with either SG 1 or SG 2 had a similar mean age and history of blood transfusion . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
Isolates belonging to Suillus granulatus ( 0 11 divergence ) were divided into three groups ( SG 1 , SG 2 and SG 3 ) ; SG 1 and SG 2 had 100 % bootstrap values . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
Two genetic lineages were distinguished within genogroup 1 , consisting of strains serologically characterized as SG 1 , and three genetic lineages were distinguished within genogroup 2 , composed of strains serologically characterized as SG 2 , SG 1 + 2 , and SG non 1 , non 2 . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
Out of 35 positive samples , 25 were classified into a genogroup SG 1 , typified by the Sapporo virus , and 9 were classified as a genogroup SG 2 , typified by the London virus . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
METHODS : The study was conducted among 30 patients with psoriasis of less than 10 mo duration ( study group 1 , sg 1 ) and 30 with psoriasis of more than 3 y duration ( study group 2 , sg 2 ) . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
Respect to the symptoms were classified , 43 ( 30 % ) as slight ; the RV isolates corresponding to these patients were 35 of serotype G1P1A SG 2 ; 4 G1P1A SG 1 and 2 ; 1 G1P1A SG Non 1 Non 1 ; 1 G 3 SG 2 ; 1 G 3 SG Non 1 and Non 2 and 1 G 3 SG 1 and 2 . 89 ( 53 % ) of patients showed moderate clinical symptoms . 58 isolates of RV were G1P1A SG 2 ; 11 G1P1A SG Non 1 Non 2 ; 9 G1P1A SG 1 and 2 ; 1 G1P1B SG 2 ; 1 G4P1A SG 2 ; 1G1 and G 4 SG 1 and 2 ; 6 G 3 SG Non 1 Non 2 ; 2 G 3 SG 2 . ^^^ The severe RV infection occurred in only 10 ( 7 % ) . 8 were serotype G1P1A SG 2 and 2 were G1P1A SG 1 and 2 . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
Although both isolated placental villous cytotrophoblast cells and chorion membrane extravillous cytotrophoblast cells contained mRNAs encoding sG 1 and sG 2 , protein expression was as predicted from the immunostains with m / sG2 present only in the invasive trophoblast subpopulation . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
The median of 45 repeats was used to divide the total into two groups ( SG 1 and SG 2 with smaller and larger [ CAG ] ( n ) fragments , respectively ) and a means analysis of the two subgroups based on [ CAG ] ( n ) demonstrated that SG 1 ( n = 10 samples at 45 repeats per sample , mean [ CAG ] ( n ) = 45 . 00 + / 0 . 00 ) was significantly smaller than SG 2 ( n = 9 , ranging from 48 to 120 repeats , mean = 70 . 22 + / 24 . 83 ; P < 0 . 005 ) . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
AFM based nanoindentations were performed to determine mechanical property profiles across resin bonded interfaces of two sequence groups : SG 1 , where the adhesive was applied and polymerized immediately after application of the primer and specimens were immersed in Hank ' s solution after 5 min , 2 h and 12 h ; or SG 2 , where specimens were placed in an incubator before the application of the adhesive , for the same time periods . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
The SG muscle had a double peaked burst activity in each masticatory cycle with one peak during the Op phase ( the SG 1 burst ) and the other during the Cl phase ( the SG 2 burst ) . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
The morphology of classification ranged from a good swelling ( SG 3 > SG 2 > SG 1 ) , to non swelling but reactive ( SG 0 R ) , and completely non swelling ( SG 0 ) . ^^^ The prevalent patterns of the swollen sperm in sequence after treatment was SG 0 > SG 1 > SG 3 > SG 2 > SG 0 R in fresh semen , and the proportion of SG 0 was almost half of the total % HOS test results . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
DLPs from the human Wa strain of virus , belonging to subgroup 2 ( SG 2 ) , possess a significantly reduced level of transcriptase activity compared to bovine UK DLPs that belong to subgroup 1 ( SG 1 ) . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
Each group was divided into 3 subgroups ( sG ) : sG 1 had no G CSF or bFGF ; sG 2 had daily subcutaneous injections of G CSF ; and sG 3 had continuous infusion of bFGF around the graft in the omentum . ^^^ Mucosal surface expansion score and overall CD 34 positive capillaries for sG 2 and sG 3 were significantly higher than for sG 1 ( both , P < . 05 ) . ^^^
Interacting proteins: P04279 and Q02383 Pubmed SVM Score :0.0
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Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
Semenogelin 1 ( SgI ) and semenogelin 2 ( SgII ) are the dominating protein components of the coagulum formed by freshly ejaculated human semen . ^^^ Semenogelin 1 ( SgI ) and semenogelin 2 ( SgII ) are the dominating protein components of the coagulum formed by freshly ejaculated human semen . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
Human semen coagulum predominantly consists of high molecular mass complexes of the seminal vesicle secreted semenogelin 1 ( SgI ) and semenogelin 2 ( SgII ) . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
Human seminal plasma contains high concentrations of prostatic acid phosphatase ( PAP ) , prostate specific antigen ( PSA ) , beta microseminoprotein ( MSP ) , semenogelin 1 ( SgI ) , and semenogelin 2 ( SgII ) , whereas only PAP and MSP are present in rodents . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
Isolation and characterization of the major gel proteins in human semen , semenogelin 1 and semenogelin 2 . ^^^ Semenogelin 1 and semenogelin 2 constitute the major gel forming proteins in human semen . ^^^ Approximately 10 20 mg semenogelin 1 and 2 5 mg semenogelin 2 were recovered from each sample with a purity exceeding 95 % as judged by SDS / PAGE . ^^^ The molecular mass of semenogelin 1 ( 49 958 Da ) and the major form of semenogelin 2 ( 63 539 Da ) measured by mass spectrometry was consistent with the reported cDNA data . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
Semenogelin 1 and semenogelin 2 , the major gel forming proteins in human semen , are substrates for transglutaminase . ^^^ The major seminal vesicle secreted proteins in human semen , semenogelin 1 and semenogelin 2 , interact non covalently and via disulphide bridges to instantly form a coagulum upon ejaculation . ^^^ However , we here show that semenogelin 1 and semenogelin 2 , both in seminal vesicle fluid and purified from semen , are substrates for factor XIIIa , the fibrin cross linking transglutaminase . ^^^ Purified semenogelin 1 and semenogelin 2 could be cross linked separately into complexes . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
The cotton top tamarin carries an extended semenogelin 1 gene but no semenogelin 2 gene . ^^^ This gene has now been cloned and identified as a semenogelin 1 gene , because of a higher number of conserved nucleotides in the human semenogelin 1 gene ( 89 % ) than in the human and the rhesus monkey semenogelin 2 genes ( 82 % ) . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
Two proteins recovered from cell surface adhesion complexes in a small cell lung carcinoma ( SCLC ) cell line were identified as fragments of the seminal plasma proteins semenogelin 1 and semenogelin 2 . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
The human seminal vesicles secrete two proteins , semenogelin 1 and semenogelin 2 , at very high concentrations . ^^^ We have now determined the nucleotide sequence of DNA located 5 13 kilobases ( kb ) downstream of the tamarin semenogelin 1 gene a region that in man is occupied by the semenogelin 2 gene . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
Two monoacidic gangliosides were isolated and purified in this study and designated as sperm ganglioside 1 and 2 ( sg 1 and sg 2 ) . ^^^ The two gangliosides , sg 1 and sg 2 , contained the same neutral sugars , galactose and glucose in molar ratio of 1 : 1 and no GalNAc except for the presence of N acetyl neuraminic acid ( NeuAc ) in sg 1 and deaminated neuraminic acid ( KDN ; 2 keto 3 deoxy D glycero D galacto nononic acid ) in sg 2 . ^^^ The complete structures of these gangliosides were determined by a combination of methylation analysis , fast atom bombardment mass spectrometry , 400 MHz one and two dimensional 1H nuclear magnetic resonance spectroscopy , fatty acid analysis , and endoglycoceramidase digestion NeuAc alpha 2 3Gal beta 1 4Glc beta 1 Cer sg 1 [ ( NeuAc ) GM 3 ] KDN alpha 2 3Gal beta 1 4Glc beta 1 Cer sg 2 [ ( KDN ) GM 3 ] where , for both sg 1 and sg 2 , the ceramide moieties ( Cer ) were found to be made up of 4 sphingenine and mainly C 16 : 0 fatty acid ( palmitate ; 95 % ) with a minor amount of C 24 : 1 fatty acyl chain ( nervonate , 5 % ) . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
Urinary sialoglucides were fractionated into two fractions ( SG 1 and SG 2 ) by Sephadex G 25 gel filtration and considerable increase in excretion of SG 1 was observed in the patients with lysosomal diseases : two to three fold increase in mucopolysaccharidosis type 2 and seven to eight fold increase in mucolipidosis . ^^^
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They were named SG 1 for the new toxin and SG 2 for the ciguatoxin like compound . ^^^ Successive filtrations on Sephadex LH 20 of SG 1 and SG 2 gave respectively a lethality to mice of 0 . 03 microgram / g and 0 . 06 microgram / g . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
On the serological relationship of fimbrial antigens between salmonella SGI and Yersinia enterocolitica and Salmonella SG 2 and Arizona ( = Salmonella SG 3 ) ( author ' s transl ) ] . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
The four groups included boars from a synthetic line ( SG 1 ) or the Large White breed ( SG 2 ) and barrows from the Large White breed ( SG 3 ) or crossbred between Large White and Meishan breeds ( SG 4 ) . ^^^ In E1 / 0 pigs , daily BW , lean , and N gain were affected ( P less than . 01 ) by group ; 566 , 471 , 374 , and 315 g / d of lean tissue gain in SG 1 , SG 2 , SG 3 , and SG 4 pigs , respectively . ^^^ At high ME intake ( E2 / pST vs E1 / 0 ) , pST increased daily BW ( +14 % ) , lean ( +27 % ) , or N ( +26 % ) gain and reduced adipose tissue ( 50 % ) gain , but the pST effect was inversely related to LTG : for N , the improvement was 2 . 8 , 7 . 1 , 7 . 0 , and 11 . 1 g / d in SG 1 , SG 2 , SG 3 , and SG 4 groups , respectively . ^^^ Energy restriction ( E3 / pST vs E2 / pST ) reduced ( P less than . 001 ) adipose tissue gain in all groups but did not affect lean tissue or N gains in SG 1 , SG 2 , and SG 3 pigs . ^^^ Energy restriction combined with pST treatment ( E3 / pST ) led to negligible amounts of fat deposited ( 40 g / d for SG 1 + SG 2 + SG 3 pigs ) and a gain : feed ratio higher than 500 g / kg ( 580 in SG 1 pigs ) . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
SgII , which has 78 % overall identity with SgI , contains eight 60 residue regions that display conspicuous internal sequence similarity , whereas SgI only contains six of these regions . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
Monoclonal antibodies specific for rotavirus subgroup 1 ( SG 1 ) and subgroup 2 ( SG 2 ) were used to analyse by enzyme immunoassay ( EIA ) the subgroups of human rotavirus isolates obtained from three different parts of India during the period September 1985 to July 1987 . ^^^ We identified one isolate which failed to react with either SG 1 or SG 2 specific monoclonal antibodies , although it reacted well with a monoclonal antibody specific for group A rotaviruses . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
In the present study , we investigated in GH4C1 cell cultures the regulation of the cell content of the Sg by immunoblotting and the relationship between the storage of Sg 1 and Sg 2 and PRL by double immunocytochemistry . ^^^ GH4C1 cells grown in the presence of gelded horse serum , a condition in which these cells contain a low level of secretory granules , contained low levels of PRL , Sg 1 , and Sg 2 . ^^^ Treatment of GH4C1 cells with a combination of 17 beta estradiol , insulin , and epidermal growth factor for 3 days , known to induce a marked increase in the number of secretory granules , increased the cell contents of PRL , Sg 1 , and Sg 2 . ^^^ Forty six percent of PRL positive cells stained coincidently for Sg 1 , while 72 % of the PRL cells were also reactive with anti Sg 2 . ^^^ To determine whether PRL storage was associated with storage of at least one of the Sg , cells were stained with anti PRL and anti Sg 1 and anti Sg 2 together . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
A comparison between the static grades and the diameter of the internal spermatic vein ( ISV ) showed that the mean value of the diameter of the ISV in SG 1 , SG 2 and SG 3 was 4 . 1 + / 0 . 66 mm , 5 . 1 + / 0 . 68 mm , and 6 . 2 + / 0 . 79 mm , respectively . ^^^ Patients with higher static grades had ISVs of significantly larger diameter ( SG 1 vs SG 2 ; p less than 0 . 05 , SG 2 vs SG 3 ; p less than 0 . 01 , SG 1 vs SG 3 ; p less than 0 . 01 , t test ) . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
Long pattern isolates usually belong to subgroup 2 ( SG 2 ) and short pattern to subgroup 1 ( SG 1 ) . ^^^
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The findings were classified into significance groups ( SG ) depending on their clinical relevance ( SG 1 : crucial ; SG 2 : of immediate relevance ; SG 3 : of future relevance ; SG 4 : of no significance ) . ^^^ In Group 2 , abnormalities were noted in 70 . 1 % of the patients , and were assigned to SG 1 in 4 . 3 % , SG 2 in 5 . 4 % , SG 3 in 32 . 5 % and SG 4 in 27 . 9 % . ^^^ After excluding the findings in the pleural cavities ( 14 . 1 % ) , aorta ( 3 . 4 % ) and biliary tract ( 1 . 2 % ) , most of the findings noted in other organs or regions of the body ( leg veins 11 . 1 % , intestine 8 . 8 % , spleen 8 . 3 % , peritoneal cavity 7 . 2 % , pancreas 1 . 7 % ) were assigned to SG 1 or SG 2 . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
In 19 out of 49 patients with legionellosis L . pneumophila SG 1 ( 3x ) , SG 2 ( 1x ) , SG 3 ( 4x ) , SG 4 ( 1x ) , SG 5 ( 5x ) , L . micdadei ( 3x ) and L . bozemanii ( 2x ) were found . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
As they could be diagnosed they belong to L . pneumophila SG 1 306 strains , SG 2 36 strains , SG 3 127 strains , SG 4 45 strain , SG 5 29 strain , SG 6 106 strains , SG 9 13 strains and SG 10 13 strains . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
It was admitted that if the areas among the particles of a size lower than 0 . 2 micron ( 200 nm ) were prevailing the granular matrix disorganization ( GMD ) was of type ( SG 1 ) ; if most of the areas were larger than 0 . 2 micron ( 200 nm ) GMD was of type 2 ( SG 2 ) and preceded the complete fusion of the granules . . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
Peripheral resistance decreased during both infusions in SG 1 but remained unaltered in CG 1 ( p less than 0 . 05 ) . 6 weeks after finishing anabolic steroid treatment ( SG 2 , n = 11 ) , in the CaCl 2 test the ejection fraction ( p less than 0 . 05 ) and stroke index were smaller than in control rats of the same age ( CG 2 , n = 12 ) . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
This new sub subgel ( SGII ) phase may be a precursor to the subgel ( SGI ) phase , and this discovery is discussed in relation to the current knowledge regarding the polymorphic gel phases of both ester and ether linked lipids with identical acyl chains . . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
A single amino acid mutation at positions 172 ( Met to Ala ) or 305 ( Asn to Ala ) was sufficient to change the subgroup specificity of the human rotavirus Wa VP 6 protein from SGII to SGI / II , since either of these mutations allowed the protein to be recognized by the SGI mAb 255 / 60 , while retaining its capacity to interact with the SGII mAb 631 / 9 . ^^^ In the case of the SGII epitope , the mutation of two contiguous amino acids ( Ala 305 Asn306 to Asn 305 Ala306 ) in the porcine rotavirus YM VP 6 protein ( SGI ) enabled the protein to be efficiently recognized by the SGII mAb 631 / 9 , while causing the YM VP 6 protein to lose its capacity to interact with mAb 255 / 60 . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
Dextrin dextranase ( EC 2 . 4 . 1 . 2 ; DDase ) , which is produced by Acetobacter capsulatus ATCC 11894 , acted on a mixture of stevioside and starch hydrolysate with isoamylase , so that the enzyme was found to convert stevioside to predominantly mono glucosyl stevioside ( SG 1 ) and di glucosyl stevioside ( SG 2 ) , and little of the stevioside initially added remained . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
The SG and VP 7 ( G ) serotype specificities could be determined for 118 ( 84 % ) of the 140 rotavirus positive stool specimens ; 4 rotavirus strains were serotype G 1 and SGII ; 1 strain was serotype G 2 and SGI+II ; 112 strains were serotype G 3 and SGII ; 1 strain was serotype G 3 and SGI ; and none of the strains was serotype G 4 . ^^^
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Values at 60 min showed an adequate anticoagulation in only 6 patients ( 24 % ) in SG 1 vs 21 ( 84 % ) in SG 2 . ^^^
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Patients treated with antifibrinolytics were divided into two groups , according to the day of hospital admission and onset of therapy , respectively between 0 and 3 days ( SG 1 ) and between 4 and 7 days from SAH ( SG 2 ) ; treated patients ( 260 cases ) received i . v . tranexamic acid ( 6 gr / day ) for at least two weeks . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
We produced three monoclonal antibodies , SG 1 , SG 2 and SG 3 , specific for human seminal gamma glutamyltransferase when characterized by enzyme linked immunosorbent assay and immunoblotting . ^^^ Seminal gamma glutamyltransferase was localized , by immunostaining , to the epithelial cells of the ductus epididymidis , seminal vesicle and prostate gland with SG 1 , those of the prostate gland with SG 2 , and those of the seminal vesicle with SG 3 . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
The two sg RNAs are 2155 ( sg 1 ) and 934 ( sg 2 ) nt in length . ^^^ ORF 3 is expressed from sg 1 RNA whereas ORFs 4 and 5 are potentially expressed from sg 2 RNA . ^^^ Time course experiments with Cynara scolymus protoplasts indicate that during AMCV infection both positive and negative strands of genomic and sg RNAs are produced and that sg 2 RNA is produced before and at a higher level than sg 1 RNA . . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
We investigated the subcellular distribution of secretogranins 1 , 2 ( Sg 1 , Sg 2 ) , and prolactin ( PRL ) by double immunogold electron microscopy in GH3B6 rat pituitary tumor cells grown in different culture conditions and in normal PRL cells in adult male rat anterior pituitary . ^^^ Co localization of Sg 1 or Sg 2 with PRL was observed in most secretory granules in GH3B6 cells and normal PRL cells , except for some secretory granules containing only Sgs in GH3B6 cells and containing only PRL in normal PRL cells . ^^^ The present study indicates a heterogeneous subcellular distribution of PRL , Sg 1 , and Sg 2 in individual secretory granules of GH3B6 cells and of normal PRL cells , pointing out the formation of different types of aggregates during the condensation of secretory products in these two PRL cell models . . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
Follow up epidemiology of this event in Manipur during 1989 1992 yielded mainly rotaviruses of more conventional characteristics ( 94 isolates of SG 2 and long electropherotype , and 90 isolates of SG 1 and short electropherotype ) , but also 6 isolates of SG 1 with long electropherotype , indicating that these viruses continue to circulate in the Manipur community . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
Patients from SG 2 had pain for a longer time ( P < 0 . 01 ) compared to patients from SG 1 with normal movement and electromyographic profiles . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
During the study , 28 patients ( SG 0 ) received S 10mg ; in 14 patients ( SG 1 ) dosage was titrated to 20mg and in 18 cases ( SG 2 ) to 40mg . ^^^ RESULTS Mean profile analysis showed different behaviour of BG , SG 0 , SG 1 and SG 2 through time for TC , TG , LDL C and VLDL C . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
A comparison of the SgI gene and the SgII gene suggests that they evolved by the duplication of an approximately 8 kb DNA segment about 61 million years ago , probably by a mechanism involving recombination between L 1 elements . . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
The HCV genotype of the patients was determined by an enzyme linked immunosorbent assay based on serological genotype 1 and 2 specific recombinant peptides ( SG 1 and SG 2 , respectively ) of the NS 4 region . ^^^ The prevalence of SG 1 and SG 2 HCV was similar in the four main islands of Japan . ^^^ The percentage of patients with SG 1 HCV increased by 7 % , while that of patients with SG 2 HCV decreased by 7 % , as liver disease progressed in severity from chronic hepatitis to carcinoma ( P < 0 . 001 ) . ^^^ Patients with either SG 1 or SG 2 had a similar mean age and history of blood transfusion . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
When the derangement of cerebral perfusion was estimated with four SPECT grades ( SG ) , 70 % of their hemispheres revealed normal ( SG 1 ) or localized decreased perfusion ( SG 2 ) . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
Hypotension and bradycardia were the most frequent adverse effects . 4 . 3 % of the adverse effects were graded as minimal ( SG 1 ) , 2 . 4 % as mild ( SG 2 ) and 0 . 7 % as moderate ( SG 3 ) . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
It is secreted as biologically active 52 kDa and a mixture of 71 and 76 kDa precursor forms , which are identical to semenogelin 1 and 2 ( Sg 1 and Sg 2 ) , respectively . ^^^ To understand the molecular mechanism underlying the inhibition of sperm motility by SPMI proteins , we expressed human Sg 1 and Sg 2 genes in insect cells using a baculovirus system . ^^^ The baculoviruses expressing full size Sg 1 and Sg 2 proteins that were N terminally tagged with a hexahistidine were selected , and were infected with Sf 21 cells . ^^^ The Sg 1 and Sg 2 proteins were purified from infected cells by column chromatography using Ni NTA resin 48 h after infection . ^^^ The full size Sg 1 and Sg 2 proteins were obtained in soluble forms . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
The sori of Dictyostelium discoideum ( strains SG 1 , SG 2 , NC 4 and V 12 ) contained more than 100 mM ammonium phosphate . ^^^ An exogenous concentration of 69 mM ammonium phosphate could maintain dormancy in spores of strains SG 1 and SG 2 for at least a week in the absence of any other inhibitory component from the sori . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
Three acidic sulfated proteins , chromogranin A ( Cg A ) , secretogranin 1 ( Sg 1 ; also called chromogranin B ; Cg B ) , and secretogranin 2 ( Sg 2 ) are well known as the major proteins in the family . ^^^ In this review , the possible roles of granin family , especially Cg A , Sg 1 ( Cg B ) and Sg 2 in the regulated secretory pathway are discussed , focusing on the sorting , aggregation of proteins in the TGN , and subsequent secretory granule formation . . ^^^
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SGI and SGII specificity were detected in 60 per cent and 20 per cent of the cases studied . ^^^
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About 94 % of the samples ( 85 / 90 ) could be subgrouped with the following results : 55 . 5 % for SG 2 , 7 . 8 % SG 1 , and 31 . 1 % for SG non 1 non 2 . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
METHODS : The proteolysis of human semenogelin 1 ( SgI ) and 2 ( SgII ) by PSA was characterized by purification of generated SgI and SgII fragments , N terminal sequencing , and mass spectrometry . ^^^ RESULTS : Eighteen cleavage sites in SgI and 16 in SgII were identified . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
Spodoptera frugiperda larvae have three midgut alpha galactosidases ( SG 1 , SG 2 , SG 3 ) partially resolved by ion exchange chromatography . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
Sg consists of two proteins , one 52 kDa ( Sg 1 ) in size , and the other a mixture of 71 and 76 kDa proteins ( Sg 2 ) . ^^^ Recombinant Sg 1 and Sg 2 proteins were obtained using a baculovirus system and then injected into a rabbit to produce the respective antibodies [ Characterization of recombinant precursor proteins of the human seminal plasma sperm motility inhibitor synthesized in insect cells , Int . ^^^ When liquefied seminal plasma was immunoblotted with the anti Sg 1 and Sg 2 antibodies , the anti Sg 2 antibody identified a wider range of the polypeptides originating from Sg than did the anti Sg 1 antibody . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
Here we utilized the technique of acid activation to identify cryptic cysteine proteinases throughout auto induced and heat induced spore germination of D . discoideum strain SG 2 and SG 1 . ^^^ The major acid activatable cysteine proteinase identified in SG 2 and SG 1 spore extracts was ddCP 38 ( D . discoideum cysteine proteinase with a molecular mass of 38 kDa ) and ddCP 48 , respectively . ^^^ Moreover , we were able to detect these cryptic cysteine proteinases in the vegetative cells and early aggregates of both strains SG 1 and SG 2 . ^^^
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Isolates belonging to Suillus granulatus ( 0 11 divergence ) were divided into three groups ( SG 1 , SG 2 and SG 3 ) ; SG 1 and SG 2 had 100 % bootstrap values . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
We examined CgA , SgI and SgII mRNA expression by reverse transcription polymerase chain reaction in rat mammary adenocarcinoma and fibroadenoma which had arisen spontaneously in aging Fischer 344 rats . ^^^ CgA and SgII mRNA were expressed in both mammary tumors , but SgI mRNA was not detected in either . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
Two genetic lineages were distinguished within genogroup 1 , consisting of strains serologically characterized as SG 1 , and three genetic lineages were distinguished within genogroup 2 , composed of strains serologically characterized as SG 2 , SG 1 + 2 , and SG non 1 , non 2 . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
Out of 35 positive samples , 25 were classified into a genogroup SG 1 , typified by the Sapporo virus , and 9 were classified as a genogroup SG 2 , typified by the London virus . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
Respect to the symptoms were classified , 43 ( 30 % ) as slight ; the RV isolates corresponding to these patients were 35 of serotype G1P1A SG 2 ; 4 G1P1A SG 1 and 2 ; 1 G1P1A SG Non 1 Non 1 ; 1 G 3 SG 2 ; 1 G 3 SG Non 1 and Non 2 and 1 G 3 SG 1 and 2 . 89 ( 53 % ) of patients showed moderate clinical symptoms . 58 isolates of RV were G1P1A SG 2 ; 11 G1P1A SG Non 1 Non 2 ; 9 G1P1A SG 1 and 2 ; 1 G1P1B SG 2 ; 1 G4P1A SG 2 ; 1G1 and G 4 SG 1 and 2 ; 6 G 3 SG Non 1 Non 2 ; 2 G 3 SG 2 . ^^^ The severe RV infection occurred in only 10 ( 7 % ) . 8 were serotype G1P1A SG 2 and 2 were G1P1A SG 1 and 2 . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
Linkage between NSP 4 genotypes and VP 6 subgroups was 100 % , NSP 4 genotype A being linked to VP 6 of subgroup 1 ( SGI ) and NSP 4 of genotype B being linked to VP 6 of SGII . ^^^
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Although both isolated placental villous cytotrophoblast cells and chorion membrane extravillous cytotrophoblast cells contained mRNAs encoding sG 1 and sG 2 , protein expression was as predicted from the immunostains with m / sG2 present only in the invasive trophoblast subpopulation . ^^^
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Among these specimens , we identified both the four globally common strains ( P [ 8 ] , G 1 subgroup 2 ( sgII ) ; P [ 4 ] , G 2 sgI ; P [ 8 ] , G 3 sgII ; and P [ 8 ] , G 4 sgII ) and six uncommon strains ( P [ 6 ] , G 4 sgII ; P [ 9 ] , G 3 sgI ; P [ 9 ] , G 6 sgI ; P [ 14 ] , G 6 sgI ; P [ 8 ] , G 9 sgII ; and P [ 8 ] , G 9 sgI ) . ^^^
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The median of 45 repeats was used to divide the total into two groups ( SG 1 and SG 2 with smaller and larger [ CAG ] ( n ) fragments , respectively ) and a means analysis of the two subgroups based on [ CAG ] ( n ) demonstrated that SG 1 ( n = 10 samples at 45 repeats per sample , mean [ CAG ] ( n ) = 45 . 00 + / 0 . 00 ) was significantly smaller than SG 2 ( n = 9 , ranging from 48 to 120 repeats , mean = 70 . 22 + / 24 . 83 ; P < 0 . 005 ) . ^^^
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AFM based nanoindentations were performed to determine mechanical property profiles across resin bonded interfaces of two sequence groups : SG 1 , where the adhesive was applied and polymerized immediately after application of the primer and specimens were immersed in Hank ' s solution after 5 min , 2 h and 12 h ; or SG 2 , where specimens were placed in an incubator before the application of the adhesive , for the same time periods . ^^^
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The SG muscle had a double peaked burst activity in each masticatory cycle with one peak during the Op phase ( the SG 1 burst ) and the other during the Cl phase ( the SG 2 burst ) . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
METHODS : The study was conducted among 30 patients with psoriasis of less than 10 mo duration ( study group 1 , sg 1 ) and 30 with psoriasis of more than 3 y duration ( study group 2 , sg 2 ) . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
In semen , the gel proteins SgI and SgII ( semenogelins 1 and 2 ) are digested by PSA ( prostate specific antigen ) , resulting in liquefaction and release of motile spermatozoa . ^^^ We characterized the binding of Zn2+ to SgI and SgII and found evidence that these proteins are involved in regulating the activity of PSA . ^^^ Intact SgI and SgII and synthetic semenogelin peptides were used in the experiments . ^^^ SgI and SgII bound Zn2+ with a stoichiometry of at least 10 mol ( mol of protein ) ( 1 ) and with an average dissociation constant of approx . 5 microM per site . ^^^ Moreover , Zn2+ inhibited PSA was activated by exposure to SgI or SgII . ^^^
Interacting proteins: Q02383 and P04279 Pubmed SVM Score :0.0
The morphology of classification ranged from a good swelling ( SG 3 > SG 2 > SG 1 ) , to non swelling but reactive ( SG 0 R ) , and completely non swelling ( SG 0 ) . ^^^ The prevalent patterns of the swollen sperm in sequence after treatment was SG 0 > SG 1 > SG 3 > SG 2 > SG 0 R in fresh semen , and the proportion of SG 0 was almost half of the total % HOS test results . ^^^
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DLPs from the human Wa strain of virus , belonging to subgroup 2 ( SG 2 ) , possess a significantly reduced level of transcriptase activity compared to bovine UK DLPs that belong to subgroup 1 ( SG 1 ) . ^^^
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Western blots detected semenogelin 1 and 2 in both RPE and neural retina while the vitreous contained only SgII . ^^^
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Each group was divided into 3 subgroups ( sG ) : sG 1 had no G CSF or bFGF ; sG 2 had daily subcutaneous injections of G CSF ; and sG 3 had continuous infusion of bFGF around the graft in the omentum . ^^^ Mucosal surface expansion score and overall CD 34 positive capillaries for sG 2 and sG 3 were significantly higher than for sG 1 ( both , P < . 05 ) . ^^^
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Here we examine the molecular evolution of the semenogelin genes ( SEMG 1 and SEMG 2 ) , which code for the predominant structural proteins in human semen . ^^^ In contrast , at both SEMG 1 and SEMG 2 we observed several gorilla haplotypes that contain at least one premature stop codon . ^^^
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