| Interacting proteins: Q15208 and P04271 |
Pubmed |
SVM Score :0.85133725 |
| In vitro , S100B binds directly to and activates Ndr in a Ca2+ dependent manner . 0.85133725^^^ The region of Ndr responsible for interaction with Ca2+ / S100B is a basic / hydrophobic motif within the N terminal regulatory domain of Ndr , and activation of Ndr by Ca2+ / S100B is inhibited by a synthetic peptide derived from this region . 0.78435788^^^ |
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| Interacting proteins: Q15208 and P04271 |
Pubmed |
SVM Score :0.0 |
| All of these residues proved to be crucial also for NDR 1 activity in vivo ; however , in contrast to Ser 281 and Thr 444 , Thr 74 seems to be involved only in binding to S100B rather than directly regulating NDR 1 activity per se . ^^^ When we added Ca ( 2+ ) / S100B , we observed an increased autophosphorylation on Ser 281 and Thr 444 , resulting in stimulation of NDR 1 activity in vitro . ^^^ |
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| Interacting proteins: Q15208 and P04271 |
Pubmed |
SVM Score :0.0 |
| Structure of the Ca2+ / S100B / NDR kinase peptide complex : insights into S 100 target specificity and activation of the kinase . ^^^ The activity of NDR kinase is modulated in a Ca ( 2+ ) / S100B dependent manner by phosphorylation of Ser 281 in the catalytic domain and Thr 444 in the C terminal regulatory domain . ^^^ S100B , which is a member of the S 100 subfamily of EF hand proteins , binds to a basic / hydrophobic sequence at the junction of the N terminal regulatory and catalytic domains ( NDR ( 62 87 ) ) . ^^^ Unlike calmodulin dependent kinases , regulation of NDR by S100B is not associated with direct autoinhibition of the active site , but rather involves a conformational change in the catalytic domain triggered by Ca ( 2+ ) / S100B binding to the junction region . ^^^ To gain further insight into the mechanism of activation of the kinase , studies have been carried out on Ca ( 2+ ) / S100B in complex with the intact N terminal regulatory domain , NDR ( 1 87 ) . ^^^ |
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| Interacting proteins: Q15208 and P04271 |
Pubmed |
SVM Score :0.0 |
| Regulation of NDR 2 protein kinase by multi site phosphorylation and the S100B calcium binding protein . ^^^ Similar to NDR 1 , the autophosphorylation of NDR 2 protein kinase was stimulated in vitro by S100B , an EF hand Ca ( 2+ ) binding protein of the S 100 family , suggesting that the two isoforms are regulated by the same mechanisms . ^^^ |
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| Interacting proteins: Q15208 and P04271 |
Pubmed |
SVM Score :0.0 |
| We showed previously that NDR is regulated by phosphorylation and by the Ca ( 2+ ) binding protein , S100B . ^^^ |
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