| Interacting proteins: Q02846 and P04271 |
Pubmed |
SVM Score :0.0 |
| In addition to recombinant bovine GCAPs , constitutively active mutants of GCAPs that activate retGC 1 in a [ Ca2+ ] independent manner and bovine brain S100b that activates retGC 1 in the presence of approximately 10 microM [ Ca2+ ] were used to investigate whether these activations take place through a similar mechanism , and whether [ Ca2+ ] is directly involved in the dimerization . ^^^ |
|
| Interacting proteins: Q02846 and P04271 |
Pubmed |
SVM Score :0.0 |
| We had shown earlier that the latter protein , which is usually called S100b ( S100betabeta or dimer of S100beta ) , also activates ROS GC but that the Vmax of activated cyclase was about 50 % lower than when stimulated by CD GCAP . ^^^ |
|
| Interacting proteins: Q02846 and P04271 |
Pubmed |
SVM Score :0.0 |
| The two main components of the system are ROS GC 1 and its Ca ( 2+ ) regulator , S100B . ^^^ Thus , transduction components involved in processing alpha ( 2D / A ) AR mediated signals are Ca ( 2+ ) , S100B , and ROS GC 1 . ^^^ |
|
| Interacting proteins: Q02846 and P04271 |
Pubmed |
SVM Score :0.0 |
| In this model , the catalytic module of ROS GC 1 at its opposite ends is flanked by GCAP 1 and S100beta modules . ^^^ |
|
| Interacting proteins: Q02846 and P04271 |
Pubmed |
SVM Score :0.0 |
| S100B modulated Ca2+ dependent ROS GC 1 transduction machinery in the gustatory epithelium : a new mechanism in gustatory transduction . ^^^ The machinery is a two component system : the Ca2+ sensor protein , S100B ; and the transducer , ROS GC 1 . ^^^ S100B senses increments in free Ca2+ , undergoes conformational change , binds to the domain amino acids ( aa ) Gly 962 Asn981 and via the transduction domain aa Ile 1030 Gln1041 activates ROS GC 1 , generating the second messenger , cyclic GMP . ^^^ |
|
| Interacting proteins: Q02846 and P04271 |
Pubmed |
SVM Score :0.0 |
| However , at micromolar concentrations of Ca ( 2+ ) , ROS GC 1 is stimulated by S100beta [ also named calcium dependent ( CD ) GCAP ] . ^^^ The present study shows that the E786D mutation has no effect on the basal catalytic activity of ROS GC 1 and on its activation by GCAP 1 and S100beta ; however , the mutated cyclase becomes more activated by GCAP 2 . ^^^ |
|
| Interacting proteins: Q02846 and P04271 |
Pubmed |
SVM Score :0.0 |
| At nanomolar Ca2+ concentrations , ROS GC 1 is activated by GCAPs and at micromolar Ca2+ concentrations , by S100beta and neurocalcin . ^^^ |
|
| Interacting proteins: Q02846 and P04271 |
Pubmed |
SVM Score :0.0 |
| The guanylate cyclase is the previously characterized ROS GC 1 from the rod outer segments and its modulator is S100beta . ^^^ A combination of peptide competition , surface plasmon resonance binding and deletion mutation studies has been used to show that these sites are specific for S100beta and not for another regulator of ROS GC 1 , guanylate cyclase activating protein 1 . ^^^ |
|
| Interacting proteins: Q02846 and P04271 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02846 and P04271 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q02846 and P04271 |
Pubmed |
SVM Score :0.0 |
| NA |
|