| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.86000191 |
| Furthermore , the repressive effect of RIP 140 could partially be overcome by overexpression of the coactivator TIF 2 , which involved a competition between TIF 2 and RIP 140 for binding to the GR . . 0.86000191^^^ |
|
| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.63382516 |
| Pull down experiments with GST / TIF2 . 4 demonstrate a direct interaction of TIF 2 with GR in a hormone dependent fashion that requires the receptor interaction domains of TIF 2 and is equally robust with agonists and most antiglucocorticoids . 0.63382516^^^ |
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| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| Overexpression of HEXIM 1 decreased ligand dependent association between GR and transcriptional intermediary factor 2 . ^^^ |
|
| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| The activity of the AF 2 transcriptional activation function of nuclear receptors ( NR ) is mediated by the partially homologous transcriptional coactivators , glucocorticoid receptor interacting protein 1 ( GRIP 1 ) / transcriptional intermediary factor 2 ( TIF 2 ) and steroid receptor coactivator 1 ( SRC 1 ) . ^^^ |
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| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| Candidate factors have been identified by the observation that changes in glucocorticoid induction parameters in CV 1 cells could be reproduced by varying the cellular levels of coactivators [ transcriptional intermediary factor 2 ( TIF 2 ) , steroid receptor coactivator 1 ( SRC 1 ) , and amplified in breast cancer 1 ( AIB 1 ) ] , comodulator [ CREB binding protein ( CBP ) ] , or corepressor [ silencing mediator for retinoid and thyroid hormone receptors ( SMRT ) ] without concomitant increases in GR . ^^^ |
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| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| Specificity and relative potency of the motif interactions were evaluated by ligand dissociation rate and the stability of chimeras of transcriptional intermediary factor 2 with full length and truncated androgen or glucocorticoid receptor . ^^^ |
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| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| Here , we report the crystal structure of the human GR ligand binding domain ( LBD ) bound to dexamethasone and a coactivator motif derived from the transcriptional intermediary factor 2 . ^^^ |
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| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| We recently reported that GR antagonist complexes bind to the coactivator TIF 2 , ( transcriptional intermediary factor 2 ) , which is consistent with the whole cell effects of coactivators being mediated by direct interactions with GR complexes . ^^^ |
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| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| CVZ mediated productive recruitment of transcriptional intermediary factor 2 to the C terminally deleted LBD requires the receptor ' s own DNA binding domain and is positively influenced by the N terminal regions of GR or progesterone receptor . ^^^ |
|
| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| The multifunctional oncoprotein beta catenin interacts with the activation function 2 domain of androgen receptor ( AR ) to stimulate androgen receptor transcriptional activity , increase sensitivity , and broaden specificity of ligand interactions . beta Catenin interacts with androgen receptor in close proximity to the binding groove for P 160 coactivators such as transcriptional intermediary factor 2 ( TIF 2 ) / glucocorticoid receptor interacting protein 1 ( GRIP 1 ) . beta Catenin can also bind directly to TIF2 / GRIP1 . ^^^ |
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| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| In contrast , the strong HDACi stimulation of GR dependent gene regulation was not accounted for by increased GR expression , but it was mimicked by overexpression of the histone acetyltransferase complex component transcriptional intermediary factor 2 . ^^^ |
|
| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| In contrast , GILZ induction by GR was largely independent of MED 1 and MED 14 , but required the p 160 cofactor transcriptional intermediary factor 2 . ^^^ |
|
| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| We also report the HXMS results for agonist bound GR LBD with the coactivator transcriptional intermediary factor 2 ( TIF 2 ) and anatagonist bound GR LBD with nuclear receptor corepressor ( NCoR ) . ^^^ |
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| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| The yeast two hybrid system was used to isolate a clone from a 17 day old mouse embryo cDNA library that codes for a novel 812 aa long protein fragment , glucocorticoid receptor interacting protein 1 ( GRIP 1 ) , that can interact with the hormone binding domain ( HBD ) of the glucocorticoid receptor . ^^^ |
|
| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| A search for possible coactivators for steroid hormone receptors resulted in identification of glucocorticoid receptor interacting protein 1 ( GRIP 1 ) . ^^^ In contrast to agonists , glucocorticoid antagonists did not promote interaction between the glucocorticoid receptor and GRIP 1 . ^^^ GRIP 1 also enhanced the hormone dependent transactivation activity of intact glucocorticoid receptor , estrogen receptor , and mineralocorticoid receptor . ^^^ Experiments with glucocorticoid receptor truncation and point mutants indicated that GRIP 1 interacted with and enhanced the activity of the C terminal AF 2 but not the N terminal AF 1 transactivation domain of the glucocorticoid receptor . ^^^ |
|
| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| One of these proteins , glucocorticoid receptor interacting protein ( GRIP 1 ) , has been shown to interact with ER and was originally hypothesized to mediate its transcriptional activity through AF 2 . ^^^ |
|
| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| As anticipated from its location , the L454S mutant interacts weakly with CoAs , such as SRC 1 and glucocorticoid receptor interacting protein 1 ( GRIP 1 ) in gel mobility shift assays and in mammalian two hybrid assays , even in the presence of the maximal dose of T 3 . ^^^ |
|
| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| Here we report that , in addition to NID , another region of coactivator GRIP 1 ( amino acids 1011 1121 ) , called the auxiliary NID ( NIDaux ) , is required in vitro and in vivo for efficient interaction with a subset of NRs , including the glucocorticoid receptor ( GR ) , androgen receptor , and retinoic acid receptor alpha . ^^^ For binding to GR , the NID and NIDaux of GRIP 1 must act in cis , but deletion of up to 144 amino acids between the two regions did not reduce binding efficiency . ^^^ Amino acids 1011 1121 of GRIP 1 also contain a p 300 interaction domain , but mutational analysis indicated that the p 300 interaction function within this region is separable from the ability to contribute to GR hormone binding domain binding . ^^^ |
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| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| Furthermore , the ER LBD and the TR compete in vitro for the related p 160 coactivators , SRC1a and GRIP 1 ( glucocorticoid receptor interacting protein 1 ) , or the putative corepressor , RIP 140 . ^^^ |
|
| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| Coexpression of other AF 2 interacting proteins , including the p 160 factors , steroid receptor coactivator 1a ( SRC 1a ) and glucocorticoid receptor interacting protein 1 ( GRIP 1 ) , had negligible effects on ER alpha / Pit 1 cooperative activation , but partially relieved RIP 140 inhibition . ^^^ |
|
| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| A novel orphan receptor , estrogen receptor related protein 3 ( ERR 3 ) , was identified by yeast two hybrid screening , using the transcriptional coactivator glucocorticoid receptor interacting protein 1 ( GRIP 1 ) as bait . ^^^ |
|
| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| Here we report that the C terminal region of p 160 coactivators glucocorticoid receptor interacting protein 1 ( GRIP 1 ) , steroid receptor coactivator 1 ( SRC 1a ) , and SRC 1e binds the N terminal AF 1 activation function of the androgen receptor ( AR ) , and p 160 coactivators can thereby enhance transcriptional activation by AR . ^^^ |
|
| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| Estrogen responses at AP 1 sites require the integrity of the ERalpha AF 1 and AF 2 activation surfaces and the complementary surfaces on the p 160 coactivator GRIP 1 ( glucocorticoid receptor interacting protein 1 ) , the NID / AF 1 region , and NR boxes . ^^^ |
|
| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| In addition , we show that members of the p 160 family of nuclear receptor coactivators , ACTR ( activator of thyroid and retinoic acid receptors ) , GRIP 1 ( glucocorticoid receptor interacting protein 1 ) , and SRC 1 ( steroid receptor coactivator 1 ) , potentiate the transcriptional activity by hERR 1 and hERR 2 in mammalian cells , and that both orphan receptors bind the coactivators in a ligand independent manner . ^^^ |
|
| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| Furthermore , using cotransfection and antisense technology we have found that , unlike SRC 1 and GRIP 1 , which are not involved in the GR complex that suppresses keratin genes , histone acetyltransferase and CBP are . ^^^ |
|
| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| Here we show the effect of a drug metabolite on the interaction of TRbeta ( 1 ) with the co activator GRIP 1 ( glucocorticoid receptor interacting protein 1 ) . ^^^ |
|
| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| Members of the p 160 coactivator family ( steroid receptor coactivator 1 ( SRC 1 ) , glucocorticoid receptor interacting protein 1 ( GRIP 1 ) , and activator of thyroid and retinoic acid receptors ( ACTR ) ) mediate transcriptional activation by nuclear receptors . ^^^ |
|
| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| Src potentiates activation functions in CREB binding protein ( CBP ) and glucocorticoid receptor interacting protein 1 ( GRIP 1 ) , and we discuss the possibility that the Src / JNK pathway enhances the activity of these coactivators , which are known to mediate AF 1 action . . ^^^ |
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| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| The glucocorticoid receptor interacting protein 1 ( GRIP 1 ) localizes in discrete nuclear foci that associate with ND 10 bodies and are enriched in components of the 26S proteasome . ^^^ The glucocorticoid receptor interacting protein 1 ( GRIP 1 ) is a member of the steroid receptor coactivator ( SRC ) family of transcriptional regulators . ^^^ |
|
| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVE : Glucocorticoid receptor interacting protein 1 ( GRIP 1 ) is a coactivator that binds to the nuclear hormone receptors in a ligand dependent manner and mediates transcriptional activation of the target genes . ^^^ |
|
| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| GRIP 1 mutants deficient in GR binding and coactivator functions were also defective for corepression , and a GRIP 1 fragment containing the GR interacting region functioned as a dominant negative for repression . ^^^ |
|
| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| Incremental amounts of CAR elicited a progressive reduction of the ER activity induced by the p 160 coactivator glucocorticoid receptor interacting protein 1 ( GRIP 1 ) . ^^^ |
|
| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| The glucocorticoid receptor ( GR ) interacting protein 1 ( GRIP 1 ) exhibits a half maximal time for fluorescent recovery ( tau ( R ) ) of 5 s , reflecting the same rapid exchange as observed for GR . ^^^ Our results indicate that GR and GRIP 1 as components of the activating complex are in a dynamic equilibrium with the promoter , and must return to the template many times during the course of transcriptional activation . . ^^^ |
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| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| To discover TNF alpha induced factors that regulate GR activity at the coactivator level , we performed yeast two hybrid screening using the NRB domain of the glucocorticoid receptor interacting protein 1 ( GRIP 1 ) as bait . ^^^ FLASH suppressed both GR transactivation and GRIP 1 enhancement of the glucocorticoid signal and inhibited the physical interaction between GR and the GRIP 1 NRB domain . ^^^ |
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| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| Alternate surfaces of transcriptional coregulator GRIP 1 function in different glucocorticoid receptor activation and repression contexts . ^^^ Members of the mammalian p 160 family , such as GRIP 1 , are known as glucocorticoid receptor ( GR ) coactivators ; at certain glucocorticoid response elements ( GREs ) , however , GRIP 1 acts as a GR corepressor . ^^^ We characterized functional interactions of GR and GRIP 1 in a repression complex where GR tethers to DNA bound activator protein 1 ( AP 1 ) , as at the human collagenase 3 gene , and tested whether the identified interactions were similar or different at other response elements . ^^^ At the AP 1 tethering GRE , we mapped the GRIP 1 corepressor activity to a domain distinct from the two known GRIP 1 activation domains ; it exhibited intrinsic GR independent repression potential when recruited to DNA via Gal 4 DNA binding domain . ^^^ The same GRIP 1 corepression domain was required for GR mediated repression at the nuclear factor kappaB ( NF kappaB ) tethering GRE of the human IL 8 gene . ^^^ |
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| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| We have previously shown that induction is mediated by the direct interaction between c Jun and ERalpha , which stabilizes a multiprotein complex containing the coactivator GRIP 1 ( glucocorticoid receptor interacting protein 1 ) . ^^^ |
|
| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| In yeast , expression of the nuclear receptor corepressor ( N CoR ) could down regulate constitutive transcriptional activation of the TR by E1A , whereas expression of the glucocorticoid receptor interacting protein 1 ( GRIP 1 ) coactivator reconstituted the E1A induced pattern of enhanced TH dependent gene activation by TR observed in mammalian cells . ^^^ |
|
| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| The p 160 coactivators , steroid receptor coactivator 1 , glucocorticoid receptor interacting protein 1 ( GRIP 1 ) and the activator of thyroid and retinoic acid receptor , have two activation domains , AD 1 and AD 2 , which transmit the activation signal from the DNA bound nuclear receptor to the chromatin and / or transcription machinery . ^^^ |
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| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| Our experiments also showed that one member of the p 160 family of steroid receptor coactivators , steroid receptor coactivator ( SRC ) 1 , but not glucocorticoid receptor interacting protein 1 ( GRIP 1 ) or amplified in breast cancer 1 ( AIB 1 ) , also functioned in gene transactivation in response to leptin treatment . ^^^ |
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| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| We found that hGRbeta suppressed the transcriptional activity of both activation function ( AF ) 1 and AF 2 of hGRalpha , indicating that hGRbeta may exert its dominant negative effect by affecting the function of coactivators that are attracted to these transactivation domains . hGRbeta bound to one of the p 160 coactivators , the glucocorticoid receptor interacting protein 1 ( GRIP 1 ) via its preserved AF 1 but not via its defective AF 2 in vitro . ^^^ |
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| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| CHO K 1 cells transfected with ERalpha or ERbeta show ERE sequence dependent differences in the functional interaction of ERalpha and ERbeta with coactivators steroid receptor coactivator 1 ( SRC 1 ) , SRC 2 ( glucocorticoid receptor interacting protein 1 ( GRIP 1 ) ) , SRC 3 amplified in breast cancer 1 ( AIB 1 ) and ACTR , cyclic AMP binding protein ( CBP ) , and steroid receptor RNA activator ( SRA ) , corepressors nuclear receptor co repressor ( NCoR ) and silencing mediator for retinoid and thyroid hormone receptors ( SMRT ) , and secondary coactivators coactivator associated arginine methyltransferase 1 ( CARM 1 ) and protein arginine methyltransferase 1 ( PRMT 1 ) . ^^^ |
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| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| Furthermore , in an in vitro reporter gene assay , dexamethasone effectively enhanced CAR / PXR mediated transactivation of the 290 bp distal enhancer module in HepG 2 cells and CV 1 cells in the presence of exogenously expressed GR and glucocorticoid receptor interacting protein 1 ( GRIP 1 ) . ^^^ Together , these results demonstrate a rational mechanistic basis for UGT1A1 induction by glucocorticoids and PXR activators , showing that activated GR enhances CAR / PXR mediated UGT1A1 regulation with the transcriptional cofactor GRIP 1 and that GR may be involved synergistically in the xenobiotic responsive regulation of UGT1A1 by CAR / PXR . . ^^^ |
|
| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| We have shown that exogenous expression of the p 160 coactivator glucocorticoid receptor interacting protein 1 ( GRIP 1 ) in hepatocytes in vivo can mediate PB independent nuclear accumulation of murine CAR ( mCAR ) . ^^^ |
|
| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| Although RA receptor gamma and glucocorticoid receptor bind to the same response element repressing transcription of keratins K6 / K16 , RA receptor interacts with the components of the EGF enhanceosome ( co activators : glucocorticoid receptor interactive protein 1 ( GRIP 1 ) / steroid receptors coactivator 1 ( SRC 1 ) ) without breaking it . ^^^ |
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| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| We previously reported that tumor necrosis factor alpha receptor and Fas associated FLASH interacts with one of the p 160 nuclear receptor coactivators , glucocorticoid receptor interacting protein ( GRIP ) 1 , at its nuclear receptor binding ( NRB ) domain , and that inhibits the transcriptional activity of the glucocorticoid receptor ( GR ) by interfering with association of GR and GRIP 1 . ^^^ Accordingly , FLASH strongly suppressed TRAM 1 and GRIP 1 induced enhancement of GR stimulated transactivation of the MMTV promoter in HCT 116 cells , while it did not affect SRC1a induced potentiation of transcription . ^^^ |
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| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| The functional synergy of the p 160 coactivators [ steroid receptor coactivator 1 , glucocorticoid receptor interacting protein ( GRIP 1 ) , or the activator for thyroid hormone and retinoid receptors ] , the histone acetyltransferases cAMP response element binding protein binding protein ( CBP ) and p 300 and the histone methyltransferase coactivator associated arginine methyltransferase ( CARM 1 ) depends on the methyltransferase activity of CARM 1 . ^^^ |
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| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| Role of activation function domain 1 , DNA binding , and coactivator GRIP 1 in the expression of partial agonist activity of glucocorticoid receptor antagonist complexes . ^^^ Furthermore , ligand dependent GRIP 1 binding to DNA bound GR complexes decreases in the order of Dex > Dex Mes > Prog > RU 486 . ^^^ Thus , the partial agonist activity of a given GR steroid complex in CV 1 cells correlates with its cell free binding of GRIP 1 . ^^^ |
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| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| Our data indicate that the enhanced potency of the hexafluoro analogs may be caused by increased DRIP 205 and glucocorticoid receptor interacting protein 1 ( GRIP 1 ) binding to VDRs and enhanced association of VDRs with DNA , as suggested by results of ChIP assays . ^^^ |
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| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| The GRIP 1 : IRF 3 interaction as a target for glucocorticoid receptor mediated immunosuppression . ^^^ Interestingly , GR and IRF 3 competed for GRIP 1 binding ; GR activation or GRIP 1 knockdown in macrophages blocked whereas GRIP 1 overexpression rescued IRF 3 dependent gene expression . ^^^ Competition with GR for GRIP 1 antagonizes IRF 3 mediated transcription , identifying the GRIP 1 : IRF 3 interaction as a novel target for glucocorticoid immunosuppression . . ^^^ |
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| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| We report here that overexpression of steroid receptor coactivator 1 ( SRC 1 ) and GR interacting protein 1 ( GRIP 1 ) enhanced repression by liganded GR , and by a GR mutant defective in repression . ^^^ Surprisingly , SRC 1 and GRIP 1 also enhanced TGFbeta induced activation from the TGFbeta responsive sequence of the PAI 1 gene by a GR independent mechanism . ^^^ Thus , the GR coactivators , SRC 1 and GRIP 1 , act as both corepressors of the glucocorticoid repression of PAI 1 gene transcription , and coactivators of TGFbeta induced activation of the PAI 1 promoter . . ^^^ |
|
| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| The effects of increasing concentrations of both GR and the coactivator TIF 2 are found to be saturable . ^^^ Furthermore , saturating levels of either GR or TIF 2 inhibit the ability of each protein , and the GME , to affect further changes in the dose response curve or partial agonist activity of antisteroids . ^^^ Support for this hypothesis comes from the observation that a fragment of the coactivator TIF 2 retaining intrinsic transactivation activity is a dominant negative inhibitor of each component ( GME , GR , and coactivator ) . ^^^ |
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| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| The crystal structure of the glucocorticoid receptor ( GR ) ligand binding domain in a ternary complex with dexamethasone and a TIF 2 coactivator peptide has been determined recently . ^^^ |
|
| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| The ability of saturating levels of GR , and added inhibitors , to prevent the actions of the three modulators ( cis acting element , GR , and TIF 2 ) but not the currently investigated C terminal fragment of E1A 13S ( E1A 133C ) indicates that E1A 133C alters GR properties via a second pathway that is downstream of the common step for the original three modulators . hSur 2 binds to E1A 133C . ^^^ |
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| Interacting proteins: Q15596 and P04150 |
Pubmed |
SVM Score :0.0 |
| Nuclear structure associated TIF 2 recruits glucocorticoid receptor and its target DNA . ^^^ Here , we investigated this process of assembly by determining the distribution of the glucocorticoid receptor ( GR ) and its coactivator , TIF 2 . ^^^ Both endogenously and ectopically expressed TIF 2 were shown to form foci in the nucleus , and GR could be recruited to the TIF 2 foci upon GR agonist but not antagonist treatment . ^^^ The TIF 2 foci could recruit GR carrying a microinjected GR responsive element . ^^^ We propose that TIF 2 provides a nuclear compartment that allows the assembly of multi protein complexes required for GR mediated gene activation . . ^^^ |
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