| Interacting proteins: Q08752 and P04150 |
Pubmed |
SVM Score :0.0 |
| Western blotting experiments following immunoprecipitation of glucocorticosteroid receptor ( GR ) complexes resulted in coprecipitation of GR , heat shock protein hsp 90 and two immunophilins : the FK 506 binding protein FKBP 59 and the CsA binding protein cyclophilin 40 ( CYP 40 ) . ^^^ Thus , both FKBP 59 and CYP 40 can be classified as hsp binding immunophilins , and their possible involvement as targets of immunosuppressants potentiating the GR mediated transcriptional activity is discussed . . ^^^ |
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| Interacting proteins: Q08752 and P04150 |
Pubmed |
SVM Score :0.0 |
| The cyclosporin A binding immunophilin CyP 40 and the FK 506 binding immunophilin hsp 56 bind to a common site on hsp 90 and exist in independent cytosolic heterocomplexes with the untransformed glucocorticoid receptor . ^^^ In this work , we show that both untransformed glucocorticoid receptor and hsp 90 heterocomplexes contain CyP 40 , a 40 kDa immunophilin of the cyclosporin A binding class . ^^^ CyP 40 is present in both native glucocorticoid receptor heterocomplexes and receptor heterocomplexes reconstituted with rabbit reticulocyte lysate , and the presence of CyP 40 in the receptor heterocomplex is stabilized by molybdate . ^^^ Our data also suggest that there are at least two types of untransformed glucocorticoid receptor hsp 90 heterocomplexes , one that contains hsp 56 and another that contains CyP 40 . ^^^ |
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| Interacting proteins: Q08752 and P04150 |
Pubmed |
SVM Score :0.0 |
| Glucocorticoid ( GC ) receptor ( GR ) complex contains heat shock proteins such as hsp 56 ( or CsA binding protein : CyP 40 ) , hsp 70 and hsp 90 . ^^^ |
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| Interacting proteins: Q08752 and P04150 |
Pubmed |
SVM Score :0.0 |
| We have shown recently that the immunophilins CyP 40 and FKBP52 / hsp56 bind to a common site on hsp 90 and that they exist in separate heterocomplexes with the glucocorticoid receptor ( GR ) . ^^^ |
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| Interacting proteins: Q08752 and P04150 |
Pubmed |
SVM Score :0.0 |
| Only a small ( but undetermined ) fraction of the native GR . hsp90 heterocomplexes contain the cyclosporin A binding immunophilin CyP 40 . ^^^ |
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| Interacting proteins: Q08752 and P04150 |
Pubmed |
SVM Score :0.0 |
| The peptidyl prolyl isomerase domain of the CyP 40 cyclophilin homolog Cpr 7 is not required to support growth or glucocorticoid receptor activity in Saccharomyces cerevisiae . ^^^ |
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| Interacting proteins: Q08752 and P04150 |
Pubmed |
SVM Score :0.0 |
| Another complex identified in T47D cytosol contained hsp 90 and the cyclosporin A binding cyclophilin of 40 kDa , CYP 40 , but not hsp 70 , PR , or GR . ^^^ |
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| Interacting proteins: Q08752 and P04150 |
Pubmed |
SVM Score :0.0 |
| The glucocorticoid receptor ( GR ) engages transient or stable interactions with chaperones ( hsp 90 , hsp 70 ) , co chaperones ( p60 / hop , hsp 40 ) and several other polypeptides such as immunophilins ( Cyp 40 , FKBP 59 ) and p 23 to achieve a high affinity ligand binding state . ^^^ |
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| Interacting proteins: Q08752 and P04150 |
Pubmed |
SVM Score :0.0 |
| In this work , we have investigated the effects of four TPR proteins ( FKBP 52 , FKBP 51 , Cyp 40 , and PP 5 ) on hormone binding function of glucocorticoid receptor ( GR ) endogenously expressed in mammalian L 929 cells . ^^^ Interestingly , no GR associated Cyp 40 was found in these cells , consistent with the ability of CsA ligand to only affect GR through the hormone export mechanism . ^^^ |
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| Interacting proteins: Q08752 and P04150 |
Pubmed |
SVM Score :0.0 |
| To induce apoptosis , GC bind to the GC receptor ( GR ) , which is regulated by various ( co ) chaperone proteins such as heat shock protein 70 ( HSP 70 ) , HSP 40 , HIP ( HSP 70 interacting protein ) , BAG 1 ( BCL 2 associated gene product 1 ) , HOP ( HSP 70 / HSP 90 Organizing protein ) , HSP 90 , P 23 , FKBP 51 , FKBP 52 and CYP 40 . ^^^ |
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| Interacting proteins: Q08752 and P04150 |
Pubmed |
SVM Score :0.0 |
| Because neither the PPIase fragment nor the TPR fragment affects the binding of FKBP 52 to the glucocorticoid receptor under conditions in which they block FKBP 52 binding to dynein or hsp 90 , respectively , different regions of FKBP 52 must determine its association with these three proteins . . ^^^ |
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| Interacting proteins: Q08752 and P04150 |
Pubmed |
SVM Score :0.0 |
| Here we show that cotransfection of 3T3 cells with the FKBP 52 PPIase domain and a green fluorescent protein ( GFP ) glucocorticoid receptor ( GR ) chimera inhibits dexamethasone dependent movement of the GFP GR from the cytoplasm to the nucleus . ^^^ After withdrawal of colcemid , microtubules reform , and PPIase inhibition of GFP GR movement is restored . ^^^ Here , we also show that native GR . hsp90 heterocomplexes immunoadsorbed from L cell cytosol contain dynein and that GR . hsp90 heterocomplexes assembled in reticulocyte lysate contain cytoplasmic dynein in a manner that is competed by the PPIase domain of FKBP52 . . ^^^ |
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| Interacting proteins: Q08752 and P04150 |
Pubmed |
SVM Score :0.0 |
| The role of FKBP 52 is to target the GR . hsp90 complex to the nucleus by binding via its PPIase domain to cytoplasmic dynein , the motor protein responsible for retrograde movement along microtubules . ^^^ |
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| Interacting proteins: Q08752 and P04150 |
Pubmed |
SVM Score :0.0 |
| The relevance of dynein for receptor function was substantiated by several experiments : 1 ) coexpression of dynamitin , which disrupts the transport complex and reduces receptor activity ; 2 ) coexpression of the PPIase domain fragment of FKBP 52 , which is known to disrupt interaction of the receptor to dynein and reduce glucocorticoid receptor function , in contrast to the corresponding fragment of FKBP 51 ; and 3 ) swapping of the PPIase domains FKBP 51 and FKBP 52 , which reverses the respective activity . ^^^ |
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| Interacting proteins: Q08752 and P04150 |
Pubmed |
SVM Score :0.0 |
| These proteins possess peptidyl prolyl isomerase ( PPIase ) and tetratricopeptide repeats ( TPR ) domains that are important for modulation of GR activity . ^^^ The goals of these studies were to determine the roles of the TPR and PPIase domains in the inhibitory activity of squirrel monkey FKBP 51 and to gain insight into structural features of squirrel monkey FKBP 51 responsible for potent inhibition of dexamethasone stimulated GR activity . ^^^ Mutations that abrogate the PPIase activity of squirrel monkey FKBP 51 had no effect on GR inhibitory activity . ^^^ |
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| Interacting proteins: Q08752 and P04150 |
Pubmed |
SVM Score :0.0 |
| The clinical parameters investigated were plaque index ( PI ) , gingival index ( GI ) , pocket probing depth ( PPD ) , loss of attachment ( LOA ) , gingival recession ( GR ) and gingival fluid flow ( GFF ) . ^^^ The results showed that there were no significant differences between the groups for PI , GI , PPD , LOA , GR or alveolar bone resorption . ^^^ |
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| Interacting proteins: Q08752 and P04150 |
Pubmed |
SVM Score :0.0 |
| Pulmonary granulomas ( GR ) with type 1 or type 2 cytokine involvement were induced in presensitized CBA mice by embolization of beads coupled to purified protein derivative ( PPD ) of Mycobacterium tuberculosis or soluble Ags derived from Schistosoma mansoni eggs ( SEA ) . ^^^ Anti IFN decreased PPD GR size by 20 % , but augmented SEA GR by nearly 50 % . ^^^ Anti TNF abrogated PPD GR area by 40 % and SEA GR by 15 % suggesting that TNF contributed more to the former . ^^^ Analysis of TNF indicated that IFN was required for maximum production by both PPD GR and SEA GR macrophages . ^^^ In LN , PPD GR and SEA GR formation was associated with T cell dependent type 1 ( IFN and IL 2 ) and type 2 ( IL 10 and IL 4 ) cytokine profiles , respectively . ^^^ |
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| Interacting proteins: Q08752 and P04150 |
Pubmed |
SVM Score :0.0 |
| Unlike PPD GR , SEA bead lesions contained eosinophils , whereas CON beads elicited only a limited mononuclear infiltrate . ^^^ PPD GR produced IFN gamma on day 2 and the draining LN produced predominantly Th 1 cytokines on days 2 and 4 . ^^^ Compared with CON GR , MPs from PPD GR were weak sources of IL 6 , whereas those of SEA GR showed enhanced and accelerated production . ^^^ In contrast , MP of PPD GR had augmented TNF producing capacity , whereas those of SEA GR showed delayed TNF production . ^^^ In vivo depletion of TNF , respectively , caused 40 and 10 % decreases in PPD GR and SEA GR but had no effect on CON GR area , indicating that TNF contributed to a greater degree to the PPD response . ^^^ |
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| Interacting proteins: Q08752 and P04150 |
Pubmed |
SVM Score :0.0 |
| Sixteen out of a total of 147 ( 10 . 9 % ) lepromatous patients developed the clinical features of GR upon the intradermal administration of PPD ; most ( 14 of 16 ) GRs occurred in bacteriologically positive cases . ^^^ In addition , patients with GR showed enhanced in vitro response to PPD , by the lymphoproliferation test and interferon gamma assay , as compared to either PPD negative individuals or PPD positive patients without GR . ^^^ |
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| Interacting proteins: Q08752 and P04150 |
Pubmed |
SVM Score :0.0 |
| Performing a strict control of the oral hygiene level and of the marginal gingival health during the whole period of study , clinical ( pocket probing depth , PPD ; probing attachment level , PAL ; gingival recession , GR ) and intrasurgical ( depth of the defect ' s intraosseous component , IOC ; level of the alveolar crest , ACL ) parameters were recorded at baseline and at the 1 year re entry procedure in each experimental site . ^^^ |
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| Interacting proteins: Q08752 and P04150 |
Pubmed |
SVM Score :0.0 |
| The therapeutic results were evaluated by assessing probing pocket depth ( PPD ) , recession of the gingival margin ( GR ) , and clinical attachment level ( CAL ) at baseline , at 1 and at 3 years after therapy . ^^^ No significant differences existed between the mean changes in PPD , GR , and CAL in the three different test groups . ^^^ |
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| Interacting proteins: Q08752 and P04150 |
Pubmed |
SVM Score :0.0 |
| The contribution of IL 4 and IL 13 to inflammation and cytokine responses was compared in mice with types 1 or 2 pulmonary granulomas ( GR ) elicited by beads bound to antigens of Mycobacteria bovis ( PPD ) or Schistosoma mansoni eggs ( SEA ) . ^^^ Type 1 PPD GR produced detectable IL 13 , but not IL 4 . ^^^ |
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| Interacting proteins: Q08752 and P04150 |
Pubmed |
SVM Score :0.0 |
| Prior to surgery and at one year after , the following parameters were evaluated by a blinded examiner : Plaque index ( PlI ) , gingival index ( GI ) , bleeding on probing ( BOP ) , probing pocket depth ( PPD ) , gingival recession ( GR ) and clinical attachment level ( CAL ) . ^^^ |
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| Interacting proteins: Q08752 and P04150 |
Pubmed |
SVM Score :0.0 |
| The following clinical parameters were evaluated : probing pocket depth ( PPD ) , clinical attachment level ( CAL ) and gingival recession ( GR ) . ^^^ |
|
| Interacting proteins: Q08752 and P04150 |
Pubmed |
SVM Score :0.0 |
| At baseline and at 6 and 12 months after surgery , the following clinical parameters were assessed by a blinded examiner : oral hygiene status ( API ) , gingival inflammation ( BOP ) , probing pocket depth ( PPD ) , clinical attachment level ( CAL ) and gingival recession ( GR ) . ^^^ |
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| Interacting proteins: Q08752 and P04150 |
Pubmed |
SVM Score :0.0 |
| Data collection : Plaque index ( PI ) , gingival index ( GI ) , probing pocket depth ( PPD ) , clinical attachment levels ( CALs ) , gingival recession ( GR ) and bleeding on probing ( BOP ) were recorded at baseline at 1st and 3rd months . ^^^ |
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| Interacting proteins: Q08752 and P04150 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q08752 and P04150 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q08752 and P04150 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q08752 and P04150 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q08752 and P04150 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q08752 and P04150 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q08752 and P04150 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q08752 and P04150 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q08752 and P04150 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q08752 and P04150 |
Pubmed |
SVM Score :0.0 |
| NA |
|